Affinage

STRAP

Serine-threonine kinase receptor-associated protein · UniProt Q9Y3F4

Length
350 aa
Mass
38.4 kDa
Annotated
2026-06-10
86 papers in source corpus 38 papers cited in narrative 37 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

STRAP is a WD40/TPR-OB-fold scaffold protein that integrates signaling, transcriptional, and post-transcriptional control across multiple pathways, broadly favoring proliferation and tumorigenicity (PMID:9856985, PMID:16778189). It was first defined as a negative regulator of TGF-β signaling: STRAP binds the type I and type II TGF-β receptors and, by recruiting and stabilizing Smad7 at the activated receptor, blocks Smad2/3 access and dampens TGF-β transcriptional responses (PMID:9856985, PMID:10757800); loss of STRAP correspondingly increases Smad2/3 phosphorylation and TGF-β-mediated growth inhibition while activating MEK/ERK signaling and downregulating p21(Cip1) (PMID:16778189). STRAP modulates the p53 tumor-suppressor axis by binding the p53 DNA-binding domain to displace Mdm2 and potentiate p53-dependent transcription and apoptosis (PMID:17916563), an activity tuned by ATM/Chk2 phosphorylation that drives its nuclear accumulation and stability (PMID:18833288) and by CBP/SIRT7-controlled acetylation (PMID:32527012); a high-resolution crystal structure established its atypical six-TPR architecture with an OB-fold domain that engages chromatin at p53 target genes (PMID:22362889). Beyond these axes, STRAP scaffolds the GSK3β/Axin destruction complex to protect β-catenin from ubiquitylation and enhance Wnt signaling and metastasis (PMID:21502811, PMID:26910283), disrupts EZH2–SUZ12 assembly to derepress NOTCH target genes and sustain cancer stem-like cells (PMID:28827371), and acts as a scaffold in innate immune signaling by bridging TAK1/IKKα/p65 and TBK1/IRF3 to promote NF-κB- and IFN-dependent cytokine production (PMID:27934954, PMID:28651742). STRAP also has a conserved post-transcriptional role as a cytoplasmic component of the SMN complex that mediates spliceosomal snRNP assembly and regulates alternative splicing of nervous-system transcripts (PMID:15848170, PMID:16159890, PMID:33230114), restrains eIF4A-dependent translation of collagen α2(I) mRNA via LARP6 (PMID:23918805), and couples Bach2 mRNA decay to translation with Csde1 during B-cell differentiation (PMID:40133358). It further controls apoptosis through redox-sensitive cysteines: iNOS-mediated S-nitrosylation at Cys152/270 releases ASK1 to activate p38 and promote apoptosis (PMID:41519199).

Mechanistic history

Synthesis pass · year-by-year structured walk · 26 steps
  1. 1998 High

    Established STRAP's founding identity by showing a WD40 protein physically associates with TGF-β receptors and inhibits their transcriptional output, defining a new receptor-proximal regulator.

    Evidence Yeast two-hybrid, co-IP and reporter assays with TβR-I/TβR-II

    PMID:9856985

    Open questions at the time
    • Mechanism of inhibition not resolved at this stage
    • No structural basis for receptor binding
  2. 2000 High

    Resolved how STRAP inhibits TGF-β signaling, showing it synergizes with Smad7 to stabilize the Smad7-receptor complex and exclude Smad2/3.

    Evidence Co-IP, mutant analysis, in vivo phosphorylation, reporter assays

    PMID:10757800

    Open questions at the time
    • Identity of the receptor-dependent kinase phosphorylating STRAP's C terminus
    • Smad6 specificity basis unexplained
  3. 2000 Medium

    Linked STRAP overexpression to oncogenic phenotypes, connecting it to MAPK activation and breast tumor biology.

    Evidence Overexpression, MAPK activity and anchorage-independent growth assays

    PMID:10646843

    Open questions at the time
    • MAPK activation mechanism not defined
    • Correlative tumor overexpression without causal test
  4. 2005 High

    Established a distinct post-transcriptional role by placing STRAP (unrip) in the cytoplasmic SMN complex required for snRNP assembly.

    Evidence Fractionation, co-IP, mass spec, snRNP assembly assay, RNAi, immunofluorescence across two labs

    PMID:15848170 PMID:16159890

    Open questions at the time
    • How STRAP partitions SMN between cytoplasm and gems mechanistically
    • Direct RNA contacts not yet mapped
  5. 2006 High

    Provided genetic loss-of-function evidence that STRAP restrains TGF-β growth inhibition and drives tumorigenicity via MEK/ERK and p21 downregulation.

    Evidence STRAP knockout MEFs, siRNA, pathway Western blots, soft agar, xenograft

    PMID:16778189

    Open questions at the time
    • Direct link between STRAP and MEK/ERK activation not biochemically defined
    • Nuclear function not addressed
  6. 2007 High

    Defined a p53-activating function, showing STRAP binds the p53 DNA-binding domain via Cys152/270 and displaces Mdm2 to potentiate p53-dependent apoptosis.

    Evidence Co-IP, cysteine mutagenesis, reporter, apoptosis and growth assays, RNAi

    PMID:17916563

    Open questions at the time
    • How a TGF-β inhibitor reconciles with p53 tumor-suppressor activation
    • Stoichiometry of the p53/NM23-H1/STRAP complex
  7. 2008 High

    Identified DNA-damage control of STRAP, showing ATM phosphorylation drives nuclear accumulation and Chk2 phosphorylation stabilizes the nuclear pool.

    Evidence Kinase assay, nuclear fractionation, export inhibition, mutant analysis

    PMID:18833288

    Open questions at the time
    • Phosphosite identity for ATM/Chk2 not fully mapped
    • Export machinery involved not identified
  8. 2008 Medium

    Reported a metabolic link, with p49/STRAP binding NDUFAB1 and altering NAD/NADH ratio and SRF deacetylation.

    Evidence Yeast two-hybrid, co-IP, co-localization, NAD/NADH and deacetylation assays

    PMID:18230186

    Open questions at the time
    • Mechanism connecting NDUFAB1 binding to NAD changes unclear
    • Relationship to scaffold functions undefined
  9. 2009 High

    Demonstrated a TGF-β-independent role of STRAP in maintaining mesenchymal morphology via WT1/E-cadherin and β-catenin localization.

    Evidence Knockout MEFs with rescue, expression profiling, immunofluorescence

    PMID:19781628

    Open questions at the time
    • How STRAP represses E-cadherin mechanistically not yet shown here
    • Link to Wnt signaling not yet established
  10. 2009 Medium

    Defined an in vitro co-chaperone activity, with p49/STRAP binding Hsp70 and inhibiting Hsp70/Hsp40 refolding.

    Evidence Co-IP, ATPase assay, refolding assay, domain mapping

    PMID:19751705

    Open questions at the time
    • Cellular relevance of chaperone inhibition untested
    • Substrate clients not identified
  11. 2011 Medium

    Connected STRAP to Wnt and Notch stability control, showing it forms a GSK3β/Axin ternary complex and stabilizes ICN3 by reducing its ubiquitination.

    Evidence Co-IP, GSK3β inhibitor studies, in vivo ubiquitination, mutant analysis

    PMID:21502811

    Open questions at the time
    • Whether STRAP acts catalytically or sterically on ubiquitination unclear
    • Single-lab finding
  12. 2012 High

    Resolved STRAP's architecture, revealing an atypical six-TPR fold with an OB-fold domain that engages chromatin at p53 targets.

    Evidence 2.05 Å crystal structure, ChIP, transcriptional assay

    PMID:22362889

    Open questions at the time
    • Structural basis of partner binding (Smad7, GSK3β) not co-crystallized
    • OB-fold nucleic-acid binding not directly demonstrated
  13. 2013 High

    Established a translational control function, showing STRAP is tethered to collagen mRNAs via LARP6 and restrains eIF4A-dependent translation of collagen α2(I).

    Evidence RNA-IP, polysome profiling, co-IP, depletion/reconstitution

    PMID:23918805

    Open questions at the time
    • Whether STRAP directly contacts eIF4A or acts via LARP6 not fully separated
    • Generality beyond collagen mRNAs
  14. 2014 High

    Defined an epigenetic gene-repression mechanism, with STRAP recruiting HDAC1 and blocking Sp1 to downregulate p21 and E-cadherin.

    Evidence Knockout/knockdown models, ChIP, ubiquitination assay, cell cycle analysis

    PMID:25483064

    Open questions at the time
    • Direct STRAP-HDAC1 contact vs indirect recruitment unresolved
    • How STRAP destabilizes Sp1 mechanistically
  15. 2014 High

    Identified MPK38-mediated Ser188 phosphorylation as a redox-dependent switch promoting STRAP's pro-apoptotic functions.

    Evidence In vitro kinase assay, cysteine mutants, co-IP, inducible shRNA, in vivo delivery

    PMID:25485581

    Open questions at the time
    • How Ser188 phosphorylation reroutes STRAP between pathways
    • Physiological trigger for MPK38 engagement
  16. 2014 High

    Revealed a mitochondrial role, with STRAP binding ATP synthase to suppress ATP production and sensitize cancer cells to apoptosis.

    Evidence Mitochondrial fractionation, co-IP, ATP and apoptosis assays, rescue

    PMID:25168243

    Open questions at the time
    • How STRAP traffics to mitochondria unknown
    • Subunit of ATP synthase bound not specified
  17. 2016 High

    Consolidated the Wnt-enhancing mechanism, showing STRAP binds GSK-3β to block β-catenin destruction-complex engagement and promote CRC metastasis.

    Evidence Co-IP, ubiquitination assay, knockdown/overexpression, in vivo metastasis

    PMID:26910283

    Open questions at the time
    • Reconciliation with STRAP's epithelial-promoting roles in other contexts
    • Direct vs scaffold-mediated GSK3β inhibition
  18. 2016 High

    Defined STRAP as a scaffold in TLR2/4-NF-κB signaling, bridging TAK1, IKKα and p65 to drive cytokine production.

    Evidence Co-IP, siRNA, overexpression, cytokine ELISA, nuclear fractionation, mutant analysis

    PMID:27934954

    Open questions at the time
    • Structural basis of multi-kinase scaffolding
    • Regulation of STRAP recruitment to the pathway
  19. 2017 High

    Extended STRAP's epigenetic role, showing it competitively disrupts EZH2-SUZ12 to derepress NOTCH genes and sustain cancer stem-like cells.

    Evidence Co-IP, H3K27me3 ChIP, silencing, NOTCH rescue, in vivo tumorigenesis

    PMID:28827371

    Open questions at the time
    • Which STRAP domain mediates PRC2 disruption
    • Whether this competes with its TPR/OB chromatin functions
  20. 2017 Medium

    Generalized the immune scaffold role to TLR3, showing STRAP bridges TBK1 and IRF3 to enhance IFN-β production.

    Evidence Co-IP, siRNA, overexpression, cytokine ELISA, mutant analysis

    PMID:28651742

    Open questions at the time
    • Direct vs indirect TBK1/IRF3 binding
    • Single-lab finding
  21. 2018 Medium

    Coupled STRAP to mRNA translational regulation in erythroblasts via the Csde1 (Unr) complex.

    Evidence Proteomics/co-IP, RNA-IP, siRNA, Western blot, qRT-PCR

    PMID:30138317

    Open questions at the time
    • Mechanism by which STRAP alters Csde1 target output without changing binding
    • Direct RNA contacts of STRAP unmapped
  22. 2019 High

    Defined STRAP/TTC5 as a negative autophagy regulator that antagonizes JMY-mediated actin nucleation at the phagophore.

    Evidence Co-IP, in vitro actin reconstitution with membrane-bound LC3, autophagy assays

    PMID:30593260

    Open questions at the time
    • How STRAP binding inhibits JMY mechanistically
    • Regulation of STRAP-JMY engagement during autophagy
  23. 2020 High

    Defined STRAP as a spliceosome-associated U2 snRNP assembly factor regulating alternative splicing of nervous-system transcripts during development.

    Evidence eCLIP-seq, knockout in mouse embryoid bodies and Xenopus, RNA-seq, snRNP assembly assay

    PMID:33230114

    Open questions at the time
    • How RNA-binding integrates with SMN-complex role
    • Splice-site selection rules from eCLIP positions
  24. 2020 Medium

    Identified acetylation control of STRAP, with CBP writing and SIRT7 erasing K147/148/156 marks that tune p53 activation and nuclear mobilization.

    Evidence In vitro deacetylation, co-IP, 3KR/3KQ mutants, p53 assays, fractionation after 5-FU

    PMID:32527012

    Open questions at the time
    • Interplay with ATM/Chk2 phosphorylation control
    • Single-lab finding
  25. 2025 High

    Demonstrated that the Csde1-STRAP complex directly couples Bach2 mRNA decay with translation to enable plasma cell differentiation.

    Evidence RNA interactome-coupled CRISPR screen, co-IP, RNA-binding, polysome analysis, genetic KO

    PMID:40133358

    Open questions at the time
    • Biochemical basis for decay-translation coupling
    • Generality beyond Bach2 mRNA
  26. 2025 High

    Defined redox regulation of STRAP-controlled apoptosis, with iNOS S-nitrosylating Cys152/270 to release ASK1 and activate p38.

    Evidence S-nitrosylation assay, co-IP, cysteine mutants, ASK1/MKK3/p38 readouts, apoptosis assay

    PMID:41519199

    Open questions at the time
    • How the same cysteines coordinate p53, MPK38 and ASK1 functions
    • Physiological iNOS-driven contexts in vivo

Open questions

Synthesis pass · forward-looking unresolved questions
  • How STRAP's many context-specific roles (TGF-β inhibition, p53 activation, Wnt/Notch enhancement, RNA processing, immune scaffolding) are selected within a single cell remains unresolved.
  • No unified model linking post-translational state (phospho/acetyl/S-nitrosyl) to pathway selection
  • Limited structural data on partner-bound complexes
  • Reconciliation of tumor-suppressive vs oncogenic activities

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 3 GO:0060090 molecular adaptor activity 3 GO:0098772 molecular function regulator activity 3 GO:0045182 translation regulator activity 2 GO:0003677 DNA binding 1
Localization
GO:0000228 nuclear chromosome 3 GO:0005634 nucleus 3 GO:0005829 cytosol 2 GO:0005739 mitochondrion 1
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-5357801 Programmed Cell Death 3 R-HSA-8953854 Metabolism of RNA 3 R-HSA-168256 Immune System 2 R-HSA-4839726 Chromatin organization 2 R-HSA-9612973 Autophagy 1
Partners
CSDE1EZH2GEMIN7GSK3BLARP6SMAD7TBK1TP53
Complex memberships
17S U2 snRNP assembly machineryCsde1-STRAP mRNPGSK3β/Axin destruction complexSMN complex

Evidence

Reading pass · 37 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 STRAP (serine-threonine kinase receptor-associated protein) is a novel WD40 domain-containing protein that interacts with TGF-β type I receptor (TβR-I) in a yeast two-hybrid system and associates with both functional TβR-I and TβR-II in vivo; overexpression of STRAP inhibits TGF-β-mediated transcriptional activation. Yeast two-hybrid, co-immunoprecipitation, transcriptional reporter assays The Journal of biological chemistry High 9856985
2000 STRAP synergizes specifically with Smad7 (but not Smad6) to inhibit TGF-β-induced transcriptional responses. STRAP associates stably with Smad7 and recruits it to the activated type I receptor, stabilizing the Smad7-receptor complex and preventing Smad2/Smad3 access. The C terminus of STRAP is required for its phosphorylation in vivo, which is dependent on TGF-β receptor kinases. Co-immunoprecipitation, transcriptional reporter assays, mutant analysis, in vivo phosphorylation assay Molecular and cellular biology High 10757800
2000 STRAP (also known as MAWD) overexpression activates mitogen-activated protein kinase signaling and promotes anchorage-independent growth of cells; the gene is frequently overexpressed in human breast tumors. Overexpression in cell lines, MAP kinase activity assay, anchorage-independent growth assay Cancer research Medium 10646843
2005 STRAP (unrip) is a component of the SMN complex, interacting directly with Gemin6 and Gemin7. Unrip also binds a subset of Sm proteins. Unrip-containing SMN complexes are necessary and sufficient to mediate assembly of spliceosomal snRNPs. Unrip localizes predominantly to the cytoplasm and is absent from nuclear gems/Cajal bodies; RNAi-mediated reduction of unrip leads to enhanced nuclear accumulation of SMN. Biochemical fractionation, co-immunoprecipitation, mass spectrometry, snRNP assembly assay, RNAi, immunofluorescence FEBS letters / Human molecular genetics High 15848170 16159890
2006 STRAP is localized in both cytoplasm and nucleus, is upregulated in colon and lung carcinomas, and stable STRAP expression activates the MEK/ERK pathway, downregulates p21(Cip1), and causes retinoblastoma protein hyperphosphorylation. STRAP knockout MEFs show increased Smad2/3 phosphorylation and TGF-β-mediated growth inhibition. STRAP enhances anchorage-independent growth and tumorigenicity in nude mice. Stable expression, siRNA knockdown, STRAP knockout MEFs, Western blot for pathway markers, soft agar assay, xenograft model Cancer research High 16778189
2007 NM23-H1 and STRAP interact directly with the DNA-binding domain of p53 (residues 113-290). Cys145 of NM23-H1 and Cys152/Cys270 of STRAP mediate p53 binding. NM23-H1 and STRAP potentiate p53-mediated transcription, apoptosis, and growth inhibition; this activation is mediated by displacement of Mdm2 from the p53-Mdm2 complex. Co-immunoprecipitation, mutant analysis, transcriptional reporter assay, apoptosis assay, growth inhibition assay, RNAi knockdown The Journal of biological chemistry High 17916563
2008 STRAP (Strap, stress-responsive activator of p300) is phosphorylated by ATM kinase upon DNA damage, which promotes its nuclear accumulation by impeding nuclear export; subsequent Chk2 phosphorylation augments STRAP protein stability once it has attained a nuclear location. Kinase assay, nuclear fractionation, mutant analysis, nuclear export inhibition EMBO reports High 18833288
2008 p49/STRAP (SRFBP1) interacts with NDUFAB1, a nuclear-encoded subunit of NADH dehydrogenase, and the two proteins co-localize in cells. Overexpression of p49/STRAP alters intracellular NAD levels and reduces the NAD/NADH ratio, and also induces deacetylation of serum response factor. Yeast two-hybrid, co-immunoprecipitation, co-localization, NAD/NADH ratio measurement, deacetylation assay BMC cell biology Medium 18230186
2009 Deletion of STRAP from mouse embryonic fibroblasts results in loss of mesenchymal morphology, upregulation of E-cadherin via WT1 induction, formation of adherens junctions, membrane localization of β-catenin, and downregulation of mesenchymal marker LEF1. Stable re-expression of STRAP reverses this epithelial phenotype, demonstrating a TGF-β-independent role of STRAP in maintenance of mesenchymal morphology. STRAP knockout MEFs, stable reconstitution, gene expression profiling, Western blot, immunofluorescence Cellular signalling High 19781628
2010 B-MYB directly interacts with STRAP via its DNA-binding domain and a region between its acidic and conserved regions. B-MYB enhances STRAP-mediated inhibition of TGF-β signaling (including modulation of receptor-Smad3 and receptor-Smad7 complexes) and dose-dependently increases STRAP-mediated stimulation of p53-induced apoptosis and cell cycle arrest. Co-immunoprecipitation, confocal microscopy, transcriptional reporter assay, apoptosis assay, mutant analysis The Journal of biological chemistry Medium 21148321
2011 STRAP binds GSK3β through its WD40 domains and forms a ternary complex with GSK3β and Axin. STRAP also binds the intracellular fragment of Notch3 (ICN3) through the ankyrin repeat domain. STRAP reduces ubiquitination of ICN3, thereby stabilizing it; this is enhanced under proteasomal inhibition conditions. Co-immunoprecipitation, GSK3β inhibitor studies, in vivo ubiquitination assay, mutant analysis Cell cycle Medium 21502811
2011 STRAP regulates c-Jun stability by decreasing its ubiquitylation and proteasomal degradation. Loss of STRAP in MEFs accelerates c-Jun turnover and reduces cyclin D1 expression; STRAP overexpression decreases c-Jun ubiquitylation in 293T cells. STRAP knockout MEFs, proteasomal inhibition, ubiquitination assay, cycloheximide chase Biochemical and biophysical research communications Medium 21397588
2012 Crystal structure of full-length STRAP (Strap/stress-responsive activator of p300) at 2.05 Å resolution reveals an atypical six tetratricopeptide repeat (TPR) protein containing an unexpected oligonucleotide/oligosaccharide-binding (OB)-fold domain. Both TPR and OB-fold domains localize to chromatin of p53 target genes and exhibit intrinsic regulatory activity necessary for the Strap-dependent p53 response. X-ray crystallography, chromatin immunoprecipitation, functional transcriptional assay Proceedings of the National Academy of Sciences of the United States of America High 22362889
2013 STRAP is tethered to collagen α1(I) and α2(I) mRNAs via interaction with LARP6 (which binds the 5' stem-loop of collagen mRNAs). STRAP prevents unrestricted translation primarily of collagen α2(I) mRNA by interacting with eIF4A. In the absence of STRAP, more collagen α2(I) mRNA co-precipitates with eIF4A and is loaded onto polysomes, causing imbalanced synthesis of α1(I)/α2(I) polypeptides, hypermodification of α1(I), and inefficient collagen trimer assembly. RNA immunoprecipitation, polysome profiling, Co-IP, Western blot, STRAP-depletion and reconstitution Molecular and cellular biology High 23918805
2013 STRAP (MAWD) inhibits proliferation and invasion in gastric cancer by inhibiting TGF-β signaling; co-expression of MAWD and MAWBP reduces Smad3 phosphorylation and nuclear translocation, and promotes expression of differentiation markers E-cadherin and pepsinogen C. Stable overexpression/knockdown, Western blot, immunofluorescence (Smad3 translocation), MTT and transwell assays, xenograft World journal of gastroenterology Medium 23687415
2014 STRAP downregulates E-cadherin and p21(Cip1) by abrogating binding of transcription factor Sp1 to its consensus binding sites. STRAP recruits HDAC1 to Sp1-binding sites in the p21(Cip1) promoter (by ChIP assay). Loss of STRAP stabilizes Sp1 by repressing its ubiquitination in G1, resulting in enhanced p21(Cip1) expression and cell cycle arrest. STRAP knockout/knockdown cell models, ChIP assay, ubiquitination assay, cell cycle analysis, microarray Cell cycle High 25483064
2014 MPK38 phosphorylates STRAP at Ser188 via direct interaction; complex formation between STRAP and MPK38 is mediated by Cys152 and Cys270 of STRAP and Cys339 and Cys377 of MPK38, indicating redox dependency. MPK38-mediated STRAP Ser188 phosphorylation promotes pro-apoptotic function of STRAP by modulating ASK1, TGF-β, p53, and PI3K/PDK1 signaling pathways. In vitro kinase assay, mutant analysis, Co-IP, inducible shRNA knockdown, adenoviral delivery in mice Cell cycle High 25485581
2014 STRAP (Strap) localizes to mitochondria where it interacts with ATP synthase, and this interaction downregulates mitochondrial ATP production. Under glucose-limiting conditions, mitochondrial STRAP sensitizes cancer cells to apoptosis in a manner rescued by extracellular ATP. STRAP also augments apoptotic effects of mitochondrial p53. Mitochondrial fractionation, co-immunoprecipitation, ATP production assay, apoptosis assay, cell rescue experiments Cell death and differentiation High 25168243
2014 p49/STRAP overexpression reduces actin content in cultured cells, results in smaller cell size, and alters expression of muscle-specific genes including SRF. Transgenic overexpression in mice causes malformations including asymmetric body cavities and cardiac morphology changes. Endogenous p49/STRAP localizes mainly to the nucleus but also distributes in the cytoplasm in close proximity to actin; transfected GFP-p49/STRAP co-localizes with nucleolin in the nucleolus. Transgenic mice, GFP transfection with co-localization, actin staining, gene expression analysis BMC cell biology Medium 25183317
2016 STRAP binds GSK-3β and reduces phosphorylation, ubiquitylation, and degradation of β-catenin by preventing its binding to the destruction complex. This leads to enhanced Wnt/β-catenin signaling, increased expression of cyclin D1, MMP2/9, and β-TrCP, and promotes CRC metastasis in vivo. Co-immunoprecipitation, ubiquitination assay, Western blot for β-catenin phosphorylation, knockdown/overexpression, in vivo metastasis model Oncotarget High 26910283
2016 STRAP acts as a scaffold protein in TLR2/4 signaling by binding TAK1, IKKα, and NF-κB subunit p65, enhancing association among these kinases and facilitating p65 phosphorylation and nuclear translocation. STRAP depletion impairs IL-6, TNF-α, and IL-1β production; STRAP overexpression increases cytokine secretion. STRAP also translocates to the nucleus after LPS stimulation to bind NF-κB, prolonging IL-6 mRNA production. The C-terminal region of STRAP is essential for this function. Co-immunoprecipitation, siRNA knockdown, overexpression, cytokine ELISA, Western blot, nuclear fractionation, mutant analysis Scientific reports High 27934954
2017 STRAP competitively disrupts association of PRC2 subunits EZH2 and SUZ12, thereby inhibiting PRC2 assembly. This antagonizes H3K27me3 formation at NOTCH pathway genes, leading to epigenetic activation of NOTCH signaling and maintenance of cancer stem-like cell subpopulations in colorectal cancer. Co-immunoprecipitation, ChIP for H3K27me3, STRAP silencing, NOTCH pathway rescue (lentiviral NICD1/HES1 expression), in vivo tumorigenesis model Cancer research High 28827371
2017 STRAP positively regulates TLR3-triggered signaling by acting as a scaffold protein that interacts with TBK1 and IRF3, enhancing IFN-β production. STRAP knockdown reduces pro-inflammatory cytokine and IFN levels in TLR3-agonist-stimulated macrophages; its C-terminus is essential for this activity. Co-immunoprecipitation, siRNA knockdown, overexpression, cytokine ELISA, mutant analysis Cellular immunology Medium 28651742
2018 STRAP interacts with MELK (maternal embryonic leucine zipper kinase) in Bax-positive colorectal cancer cells. Sanguinarine dephosphorylates STRAP and MELK and disrupts their association, triggering intrinsic (Bax-dependent) apoptosis with increased mitochondrial ROS and MOMP. Co-immunoprecipitation, immunofluorescence, in vitro kinase activity assay, Western blot, flow cytometry, orthotopic in vivo model BMC cancer Medium 29783958
2018 STRAP associates with Csde1 (Unr) in erythroblasts; Strap knockdown alters mRNA and/or protein expression of several Csde1-bound transcripts involved in translational regulation (including Hmbs, eIF4g3, Pabpc4, Vim, Elavl1) but does not alter the pool of transcripts bound by Csde1. Proteomics/Co-IP, RNA immunoprecipitation, siRNA knockdown, Western blot, qRT-PCR PloS one Medium 30138317
2019 During autophagy, TTC5/STRAP binds JMY (junction mediating and regulatory protein) and antagonizes JMY's actin nucleation activity, acting as a negative autophagy regulator. LC3 recruits JMY to the phagophore membrane and stimulates JMY-mediated actin filament assembly; an in vitro reconstitution system demonstrated that membrane-bound LC3 is sufficient for this recruitment and activation. Co-immunoprecipitation, in vitro actin reconstitution assay with membrane-bound LC3, autophagy functional assays Autophagy High 30593260
2020 STRAP is a spliceosome-associated factor involved in assembly of 17S U2 snRNP proteins. Upon Strap deletion in mouse embryoid bodies, numerous alternative splicing events occur particularly for nervous system development transcripts. STRAP preferably targets transcripts for nervous system development by eCLIP-seq and regulates alternative splicing through preferred binding positions on neuronal-specific gene transcripts (Nnat, Mark3). In Xenopus, loss of Strap impedes lineage differentiation, delays neural tube closure, and alters exon skipping. eCLIP-seq, Strap knockout in mouse embryoid bodies, RNA-seq for alternative splicing, Xenopus loss-of-function, U2 snRNP assembly assay Nature communications High 33230114
2020 STRAP (p49/STRAP) overexpression suppresses PGC-1α, mitofusin-1, and mitofusin-2 expression at mRNA and protein levels, reduces mitochondrial size, mitochondrial membrane potential, and oxygen consumption rate, and causes deacetylation of histone H4K16. Transgenic overexpression, Western blot, qRT-PCR, mitochondrial size measurement, oxygen consumption rate measurement, histone acetylation assay Experimental gerontology Medium 27337995
2020 SIRT7 deacetylates STRAP at lysines 147, 148, and 156; acetylation of these residues is written by CBP acetyltransferase. Hypo- or hyperacetylation mutations at these sites (3KR or 3KQ) influence STRAP-mediated activation and stabilization of p53. Following 5-FU treatment, STRAP is mobilized from cytoplasm to nucleus with enhanced acetylation. In vitro deacetylation assay, Co-IP, mutant analysis (3KR/3KQ), p53 activity assays, nuclear fractionation after drug treatment International journal of molecular sciences Medium 32527012
2020 GDF5 increases expression of STRAP and NME1 in SH-SY5Y neuronal cells. Expression of both STRAP and NME1 is necessary and sufficient for promotion of neurite growth by GDF5 in SH-SY5Y cells. Proteomics, siRNA knockdown, overexpression, neurite growth assay, in vivo GDF5 overexpression in rat brain iScience Medium 32853992
2024 circPCNXL2 directly binds STRAP and induces interaction between STRAP and MEK1/2, resulting in activation of ERK/MAPK pathways and tumor promotion in intrahepatic cholangiocarcinoma. RNA pulldown, mass spectrometry, RNA immunoprecipitation, luciferase reporter assay, Co-IP, xenograft model Molecular cancer Medium 38365721
2025 The Csde1-Strap complex directly couples Bach2 mRNA decay with translation during B cell differentiation. Strap interacts with Csde1 and both bind Bach2 mRNA; in the absence of either protein, Bach2 translation is decoupled from mRNA decay, leading to elevated and prolonged Bach2 protein expression and impaired plasma cell differentiation. RNA interactome capture-coupled CRISPR/Cas9 functional screen, Co-IP, RNA-binding assay, polysome analysis, genetic KO of Csde1 and Strap Nature communications High 40133358
2025 USP38 stabilizes STRAP via deubiquitination, thereby enhancing TGF-β/SMAD signaling. STRAP knockdown reverses the pro-fibrotic effects induced by USP38 overexpression in atrial cells. Co-immunoprecipitation, ubiquitination assay, immunofluorescence, cardiomyocyte-specific KO/TG mice, siRNA knockdown Molecular medicine Medium 40514673
2025 iNOS mediates S-nitrosylation of STRAP specifically at Cys152 and Cys270 (the same residues required for STRAP-ASK1 interaction). S-nitrosylation of STRAP disrupts the STRAP-ASK1 interaction, increases ASK1 activity, activates the MKK3-p38 pathway, and enhances hydrogen peroxide-induced apoptosis. STRAP specifically interacts with iNOS but not with eNOS or nNOS. S-nitrosylation assay, Co-IP, mutant analysis (STRAP-C152/270S), ASK1 activity assay, Western blot for MKK3/p38 phosphorylation, apoptosis assay The Journal of biological chemistry High 41519199
2009 p49/STRAP interacts with the beta-sandwich domain of Hsp70, reduces the ATP-hydrolytic activity of Hsp70 stimulated by Hsp40, and inhibits the refolding activity of the Hsp70/Hsp40 system, functioning as a bona fide co-chaperone. Co-immunoprecipitation, ATPase assay, protein refolding assay, domain mapping Biochemical and biophysical research communications Medium 19751705
2006 p49/STRAP specifically interacts with an acidic motif (Q7IGSEDG) in the N-terminus of GLUT4 and co-localizes with GLUT4 and ER-resident protein calnexin in rat adipose cells. Overexpression of the GLUT4-binding domain of p49 reduces protein synthesis and cell-surface expression of GLUT4 but not GLUT8. Yeast two-hybrid, confocal immunofluorescence, mutant GLUT4 (ED/QN) analysis, cell-surface GLUT4 quantification Biochemical and biophysical research communications Medium 16647043
2024 STRAP interacts with TBK1 to activate type I IFN signaling during pseudorabies virus (PRV) infection. Both the C-terminal and WD40 7-6 domains of STRAP contribute to this function. TBK1 competes with PRV-UL50 for binding to STRAP, and STRAP impedes the degradation of TBK1 mediated by PRV-UL50. Co-immunoprecipitation, domain mutant analysis, IFN reporter assay, competition binding assay Virology journal Medium 39182136

Source papers

Stage 0 corpus · 86 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 Suspension trapping (STrap) sample preparation method for bottom-up proteomics analysis. Proteomics 352 24678027
2018 S-Trap, an Ultrafast Sample-Preparation Approach for Shotgun Proteomics. Journal of proteome research 270 30114372
2001 STRAP: editor for STRuctural Alignments of Proteins. Bioinformatics (Oxford, England) 191 11301311
2000 STRAP and Smad7 synergize in the inhibition of transforming growth factor beta signaling. Molecular and cellular biology 148 10757800
2018 Comparison of In-Solution, FASP, and S-Trap Based Digestion Methods for Bottom-Up Proteomic Studies. Journal of proteome research 142 29754492
1998 Identification of STRAP, a novel WD domain protein in transforming growth factor-beta signaling. The Journal of biological chemistry 104 9856985
2019 Suspension Trapping (S-Trap) Is Compatible with Typical Protein Extraction Buffers and Detergents for Bottom-Up Proteomics. Journal of proteome research 88 30761899
2007 NM23-H1 tumor suppressor and its interacting partner STRAP activate p53 function. The Journal of biological chemistry 82 17916563
2017 STRAP Promotes Stemness of Human Colorectal Cancer via Epigenetic Regulation of the NOTCH Pathway. Cancer research 75 28827371
2005 Unrip, a factor implicated in cap-independent translation, associates with the cytosolic SMN complex and influences its intracellular localization. Human molecular genetics 70 16159890
2006 Oncogenic function of a novel WD-domain protein, STRAP, in human carcinogenesis. Cancer research 67 16778189
2005 Unrip is a component of SMN complexes active in snRNP assembly. FEBS letters 66 15848170
2000 Molecular cloning and characterization of human MAWD, a novel protein containing WD-40 repeats frequently overexpressed in breast cancer. Cancer research 61 10646843
2023 Stratification of biological therapies by pathobiology in biologic-naive patients with rheumatoid arthritis (STRAP and STRAP-EU): two parallel, open-label, biopsy-driven, randomised trials. The Lancet. Rheumatology 48 38251532
2018 Sanguinarine triggers intrinsic apoptosis to suppress colorectal cancer growth through disassociation between STRAP and MELK. BMC cancer 42 29783958
2013 Serine-threonine kinase receptor-associated protein (STRAP) regulates translation of type I collagen mRNAs. Molecular and cellular biology 41 23918805
2023 Squalene epoxidase promotes hepatocellular carcinoma development by activating STRAP transcription and TGF-β/SMAD signalling. British journal of pharmacology 36 36581319
2019 Self-Assembled STrap for Global Proteomics and Salivary Biomarker Discovery. Journal of proteome research 36 30848925
2004 DNA binding provides a molecular strap activating the adenovirus proteinase. Molecular & cellular proteomics : MCP 30 15220401
2016 Novel role of STRAP in progression and metastasis of colorectal cancer through Wnt/β-catenin signaling. Oncotarget 27 26910283
2011 TGF-beta-dependent and -independent roles of STRAP in cancer. Frontiers in bioscience (Landmark edition) 27 21196161
2011 Role of STRAP in regulating GSK3β function and Notch3 stabilization. Cell cycle (Georgetown, Tex.) 27 21502811
2004 STRAP is a strong predictive marker of adjuvant chemotherapy benefit in colorectal cancer. Neoplasia (New York, N.Y.) 27 15720808
2024 CircPCNXL2 promotes tumor growth and metastasis by interacting with STRAP to regulate ERK signaling in intrahepatic cholangiocarcinoma. Molecular cancer 26 38365721
2014 Investigation of in vitro anticancer and DNA strap interactions in live cells using carboplatin type Cu(II) and Zn(II) metalloinsertors. European journal of medicinal chemistry 26 25128669
2014 Oncogenic STRAP functions as a novel negative regulator of E-cadherin and p21(Cip1) by modulating the transcription factor Sp1. Cell cycle (Georgetown, Tex.) 24 25483064
2014 A crucial role for the phosphorylation of STRAP at Ser(188) by MPK38 in STRAP-dependent cell death through ASK1, TGF-β, p53, and PI3K/PDK1 signaling pathways. Cell cycle (Georgetown, Tex.) 23 25485581
2021 Carbaporphyrin Dimers That Bear a Rigid Naphthalene Motif as an Internal Strap. Organic letters 18 33577339
2015 Calix[4]pyrroles with Shortest Possible Strap: Exclusively Selective toward Fluoride Ion. Organic letters 18 26313641
2014 Overexpression of p49/STRAP alters cellular cytoskeletal structure and gross anatomy in mice. BMC cell biology 18 25183317
2013 MAWBP and MAWD inhibit proliferation and invasion in gastric cancer. World journal of gastroenterology 18 23687415
2008 Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein. BMC cell biology 18 18230186
2008 ATM and Chk2 kinase target the p53 cofactor Strap. EMBO reports 18 18833288
2003 KISS for STRAP: user extensions for a protein alignment editor. Bioinformatics (Oxford, England) 18 14668241
2020 STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells. Nature communications 17 33230114
2018 Dual Roles of Serine-Threonine Kinase Receptor-Associated Protein (STRAP) in Redox-Sensitive Signaling Pathways Related to Cancer Development. Oxidative medicine and cellular longevity 17 29849900
2012 The p53 cofactor Strap exhibits an unexpected TPR motif and oligonucleotide-binding (OB)-fold structure. Proceedings of the National Academy of Sciences of the United States of America 17 22362889
2015 C-STrap Sample Preparation Method--In-Situ Cysteinyl Peptide Capture for Bottom-Up Proteomics Analysis in the STrap Format. PloS one 16 26407052
2025 Deep molecular profiling of synovial biopsies in the STRAP trial identifies signatures predictive of treatment response to biologic therapies in rheumatoid arthritis. Nature communications 15 40603860
2009 Developmental morphology of strap-shaped gametophytes of Colysis decurrens: a new look at meristem development and function in fern gametophytes. Annals of botany 15 19812067
2020 SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability. International journal of molecular sciences 14 32527012
2020 STRAP and NME1 Mediate the Neurite Growth-Promoting Effects of the Neurotrophic Factor GDF5. iScience 14 32853992
2010 B-MYB positively regulates serine-threonine kinase receptor-associated protein (STRAP) activity through direct interaction. The Journal of biological chemistry 14 21148321
2009 Serine threonine receptor-associated protein (STRAP) plays a role in the maintenance of mesenchymal morphology. Cellular signalling 14 19781628
2023 Suspension TRAPping Filter (sTRAP) Sample Preparation for Quantitative Proteomics in the Low µg Input Range Using a Plasmid DNA Micro-Spin Column: Analysis of the Hippocampus from the 5xFAD Alzheimer's Disease Mouse Model. Cells 13 37174641
2021 Comparison of protein characterization using In solution and S-Trap digestion methods for proteomics. Biochemical and biophysical research communications 13 34922203
2016 Does p49/STRAP, a SRF-binding protein (SRFBP1), modulate cardiac mitochondrial function in aging? Experimental gerontology 13 27337995
2014 Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase. Cell death and differentiation 13 25168243
2005 Structural interpretation of mutations and SNPs using STRAP-NT. Protein science : a publication of the Protein Society 13 16322575
2021 Folding in Place: Design of β-Strap Motifs to Stabilize the Folding of Hairpins with Long Loops. The Journal of organic chemistry 11 34499510
2019 Regulation of JMY's actin nucleation activity by TTC5/STRAP and LC3 during autophagy. Autophagy 11 30593260
2018 Strap associates with Csde1 and affects expression of select Csde1-bound transcripts. PloS one 11 30138317
2018 Oncogenic roles of serine-threonine kinase receptor-associated protein (STRAP) in osteosarcoma. Cancer chemotherapy and pharmacology 11 30276452
2021 Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Knockout Decreases the Malignant Phenotype in Neuroblastoma Cell Lines. Cancers 10 34206917
2018 Oncogenic STRAP Supports Hepatocellular Carcinoma Growth by Enhancing Wnt/β-Catenin Signaling. Molecular cancer research : MCR 10 30257989
2016 STRAP Acts as a Scaffolding Protein in Controlling the TLR2/4 Signaling Pathway. Scientific reports 10 27934954
2020 S-Trap Eliminates Cell Culture Media Polymeric Surfactants for Effective Proteomic Analysis of Mammalian Cell Bioreactor Supernatants. Journal of proteome research 9 32207952
2020 Developing a mass spectrometry-based assay for the ovarian cancer biomarker CA125 (MUC16) using suspension trapping (STrap). Analytical and bioanalytical chemistry 9 32215689
2017 STRAP positively regulates TLR3-triggered signaling pathway. Cellular immunology 9 28651742
2015 Mitogen-activated protein kinase activator with WD40 repeats (MAWD) and MAWD-binding protein induce cell differentiation in gastric cancer. BMC cancer 9 26373288
2011 STRAP regulates c-Jun ubiquitin-mediated proteolysis and cellular proliferation. Biochemical and biophysical research communications 9 21397588
2013 STRAP PTM: Software Tool for Rapid Annotation and Differential Comparison of Protein Post-Translational Modifications. Current protocols in bioinformatics 8 25422678
1999 Influence of girth strap tensions on athletic performance of racehorses. Equine veterinary journal. Supplement 8 10659222
2025 Ubiquitin-specific protease 38 modulates atrial fibrillation susceptibility in chronic kidney disease via STRAP stabilization and activation of TGF-β/SMAD signaling. Molecular medicine (Cambridge, Mass.) 7 40514673
2023 Comparison of SPEED, S-Trap, and In-Solution-Based Sample Preparation Methods for Mass Spectrometry in Kidney Tissue and Plasma. International journal of molecular sciences 7 37047281
2017 Downregulation of STRAP promotes tumor growth and metastasis in hepatocellular carcinoma via reducing PTEN level. IUBMB life 7 29283497
2006 HotSwap for bioinformatics: a STRAP tutorial. BMC bioinformatics 7 16469097
2008 Strap: a versatile transcription co-factor. Cell cycle (Georgetown, Tex.) 6 18719389
2006 Identification and characterization of p49/STRAP as a novel GLUT4-binding protein. Biochemical and biophysical research communications 6 16647043
2022 Serine-Threonine Kinase Receptor Associate Protein (STRAP) confers an aggressive phenotype in neuroblastoma via regulation of Focal Adhesion Kinase (FAK). Journal of pediatric surgery 5 35272839
2016 X-radiation enhances the collagen type I strap formation and migration potentials of colon cancer cells. Oncotarget 5 27655687
2015 Keratinized strap cells: a rare cytological atypia resembles Anitschkow cells, in human oral neoplasm. International journal of clinical oncology 5 26160758
2024 Protocol for feeding strategy and proteomics analysis of zebrafish Danio rerio using S-trap and iTRAQ techniques. STAR protocols 4 39661508
2021 Characterizing the Host Coral Proteome of Platygyra carnosa Using Suspension Trapping (S-Trap). Journal of proteome research 4 33630606
2017 p49/STRAP, a Serum Response Factor Binding Protein (SRFBP1), Is Involved in the Redistribution of Cytoskeletal F-Actin Proteins during Glucose Deprivation. The journal of nutrition, health & aging 4 29188873
2009 Interaction of Hsp70 with p49/STRAP, a serum response factor binding protein. Biochemical and biophysical research communications 4 19751705
2023 "Out of the blue": A qualitative study exploring the experiences of women and next of kin receiving unexpected results from BRA-STRAP research gene panel testing. Journal of genetic counseling 3 37864663
2020 STRAP reduces endoplasmic reticulum stress and apoptosis in cardiomyocytes and attenuates myocardial ischemia-reperfusion injury by activating PI3K/PDK1/Akt signaling pathway. European review for medical and pharmacological sciences 3 32373981
2013 Padded self-adhesive strap immobilization following newborn bladder exstrophy closure: the Utah straps. The Journal of urology 3 23810641
2025 A Csde1-Strap complex regulates plasma cell differentiation by coupling mRNA translation and decay. Nature communications 2 40133358
2024 STRAP upregulates antiviral innate immunity against PRV by targeting TBK1. Virology journal 1 39182136
2024 STRAP Knockdown Inhibits Migration and Growth of Non-Small Cell Lung Cancer. Bulletin of experimental biology and medicine 1 39455498
2026 S-nitrosylation of the scaffold protein STRAP enhances oxidative stress-induced apoptosis. The Journal of biological chemistry 0 41519199
2026 Structural Basis for Mechanical Coupling in Hsp90: Hinge Flexibility Coordinates ATP Gate Closure and β-Strap Release. Biochemistry 0 42234987
2025 The Role of Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Signaling in Cancer. Cells 0 40558481
2012 StRAP: an integrated resource for profiling high-throughput cancer genomic data from stress response studies. PloS one 0 23284744

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