Affinage

STRAP

Serine-threonine kinase receptor-associated protein · UniProt Q9Y3F4

Length
350 aa
Mass
38.4 kDa
Annotated
2026-04-28
85 papers in source corpus 34 papers cited in narrative 34 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

STRAP (serine-threonine kinase receptor-associated protein/TTC5/Unrip) is a multifunctional scaffold protein that integrates TGF-β, Wnt/β-catenin, p53, innate immune, and spliceosomal pathways. Structurally, STRAP adopts a six-TPR plus OB-fold architecture that provides an extended superhelical surface for diverse protein–protein and protein–nucleic acid interactions (PMID:22362889). In TGF-β signaling, STRAP recruits Smad7 to activated type I receptors, stabilizing the inhibitory Smad7–receptor complex and blocking Smad2/3 access (PMID:10757800); it activates Wnt/β-catenin signaling by binding GSK-3β and preventing β-catenin phosphorylation and degradation (PMID:26910283); it potentiates p53 transcriptional activity by displacing Mdm2 from p53, a function modulated by ATM/Chk2-dependent nuclear accumulation and CBP/SIRT7-regulated acetylation (PMID:17916563, PMID:18833288, PMID:32527012). Additionally, STRAP is an integral subunit of the SMN complex—interacting with Gemin6/7 to mediate spliceosomal snRNP assembly (PMID:15848170)—regulates collagen mRNA translation through LARP6 and eIF4A (PMID:23918805), couples Bach2 mRNA decay with translation via the Csde1–Strap complex to control B cell differentiation (PMID:40133358), inhibits JMY-mediated actin nucleation during autophagy (PMID:30593260), scaffolds TLR2/4 and TLR3 innate immune signaling through TAK1–IKKα–p65 and TBK1–IRF3 complexes (PMID:27934954, PMID:28651742), and localizes to mitochondria where it suppresses ATP synthase-dependent oxidative phosphorylation (PMID:25168243).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1998 High

    The discovery that STRAP is a WD40-domain protein interacting with TGF-β receptors and inhibiting TGF-β transcription established STRAP as a negative regulator of TGF-β signaling, opening the question of its mechanism of inhibition.

    Evidence Yeast two-hybrid screen and co-IP with TβR-I/TβR-II plus transcriptional reporter assays in mammalian cells

    PMID:9856985

    Open questions at the time
    • Mechanism of inhibition not yet defined
    • No structural information
    • Physiological relevance in vivo unknown
  2. 2000 High

    Demonstrating that STRAP recruits Smad7 to the activated type I receptor and stabilizes the inhibitory complex—blocking Smad2/3 access—defined the molecular mechanism of STRAP-mediated TGF-β pathway inhibition.

    Evidence Co-IP, deletion mutagenesis, and reporter assays showing STRAP–Smad7 cooperativity and receptor complex formation

    PMID:10757800

    Open questions at the time
    • In vivo requirement not yet demonstrated
    • Post-translational regulation of STRAP unknown
    • Structural basis of Smad7 interaction unresolved
  3. 2005 High

    Identification of STRAP (Unrip) as a stable subunit of the SMN complex—binding Gemin6/7 and mediating spliceosomal snRNP assembly in vitro—revealed a second major function distinct from TGF-β signaling and raised the question of how one scaffold serves both pathways.

    Evidence Biochemical reconstitution of snRNP assembly, co-IP defining Gemin6/7 interaction, and RNAi-mediated knockdown showing altered SMN nuclear accumulation

    PMID:15848170 PMID:16159890

    Open questions at the time
    • No structural detail of STRAP within the SMN complex
    • How STRAP partitions between SMN and receptor signaling complexes unknown
    • Whether snRNP assembly role is relevant to disease phenotypes not tested
  4. 2006 High

    Using STRAP-knockout MEFs and stable overexpression, STRAP was shown to activate the MEK/ERK pathway and suppress p21 and TGF-β-mediated growth arrest, establishing its role as a proliferative signal beyond TGF-β receptor inhibition.

    Evidence STRAP-knockout MEFs showing enhanced Smad2/3 phosphorylation; overexpression activating ERK and downregulating p21

    PMID:16778189

    Open questions at the time
    • Direct ERK pathway target of STRAP unidentified
    • Whether STRAP is an oncogene in vivo not addressed
  5. 2007 High

    Identifying that STRAP and NM23-H1 potentiate p53 activity by displacing Mdm2 from the p53 complex—mapping interaction to specific cysteine residues—established STRAP as a p53 co-activator and connected TGF-β and p53 pathways through a shared scaffold.

    Evidence Co-IP with cysteine mutagenesis, transcriptional reporters, and apoptosis assays

    PMID:17916563

    Open questions at the time
    • No in vivo p53 tumor suppression phenotype for STRAP
    • Whether STRAP-p53 interaction is constitutive or damage-dependent unknown
  6. 2008 High

    ATM-dependent phosphorylation driving STRAP nuclear accumulation, followed by Chk2-dependent stabilization, defined how the DNA damage response regulates STRAP subcellular distribution to enhance its nuclear p53 co-activator function.

    Evidence In vitro kinase assays, mutational analysis, and nuclear/cytoplasmic fractionation with live-cell imaging

    PMID:18833288

    Open questions at the time
    • Phosphorylation sites on STRAP not all mapped
    • Whether nuclear STRAP engages chromatin directly was unknown at this point
  7. 2009 High

    STRAP deletion caused mesenchymal-to-epithelial transition in MEFs with E-cadherin upregulation and β-catenin membrane relocalization, revealing STRAP as a master regulator of epithelial–mesenchymal identity beyond individual signaling pathways.

    Evidence STRAP-knockout MEFs with gene expression profiling, immunofluorescence, and phenotypic rescue by STRAP re-expression

    PMID:19781628

    Open questions at the time
    • Whether STRAP controls EMT in vivo during development or cancer progression not shown
    • Upstream signals controlling STRAP in this context unknown
  8. 2011 High

    Demonstrating that STRAP binds GSK-3β/Axin and stabilizes Notch3 ICN and c-Jun by reducing their ubiquitylation established STRAP as a general protein stabilizer acting through the GSK-3β–destruction complex axis and ubiquitin-proteasome modulation.

    Evidence Co-IP defining STRAP–GSK-3β–Axin ternary complex, ubiquitination assays for ICN3 and c-Jun, and STRAP-knockout/rescue MEFs

    PMID:21397588 PMID:21502811

    Open questions at the time
    • Whether STRAP directly inhibits an E3 ligase or acts indirectly through GSK-3β unclear
    • Structural basis of STRAP–GSK-3β interaction unresolved
  9. 2012 High

    The crystal structure at 2.05 Å revealed an unexpected TPR–OB-fold architecture rather than a canonical WD40 domain, redefining STRAP's structural classification and explaining its capacity for diverse protein and chromatin interactions.

    Evidence X-ray crystallography of full-length Strap, ChIP on p53 target genes, and functional reporter assays

    PMID:22362889

    Open questions at the time
    • Structures of STRAP in complex with any partner remain unsolved
    • How the OB-fold domain contacts DNA specifically unknown
  10. 2013 High

    STRAP was shown to control collagen mRNA translation by being tethered via LARP6 to collagen 5′ stem-loops and restraining eIF4A-dependent ribosome loading, revealing a post-transcriptional regulatory function critical for balanced collagen trimer assembly.

    Evidence RNA pulldown, polysome profiling, co-IP, siRNA knockdown, and collagen secretion rescue

    PMID:23918805

    Open questions at the time
    • How STRAP inhibits eIF4A helicase activity mechanistically unknown
    • Whether this function operates in fibrotic disease contexts not tested
  11. 2014 High

    Multiple studies converged to define STRAP's post-translational regulation: MPK38 phosphorylation at Ser188 switches STRAP from anti-apoptotic to pro-apoptotic, STRAP recruits HDAC1 to repress Sp1-dependent transcription, and mitochondrial STRAP suppresses ATP synthase to modulate energy metabolism and apoptosis.

    Evidence In vitro kinase assays with Ser188 mutagenesis, ChIP for HDAC1/Sp1 at p21 promoter, mitochondrial fractionation with ATP production assays

    PMID:25168243 PMID:25483064 PMID:25485581

    Open questions at the time
    • Whether MPK38–STRAP axis operates in vivo under physiological stress unknown
    • How STRAP is targeted to mitochondria undefined
    • Relationship between mitochondrial and nuclear STRAP pools unexplored
  12. 2016 High

    STRAP was established as a scaffold for innate immune signaling: it bridges TAK1–IKKα–p65 downstream of TLR2/4 and promotes cytokine production, while also activating Wnt/β-catenin signaling by binding GSK-3β to prevent β-catenin destruction complex formation in colorectal cancer.

    Evidence Co-IP defining TAK1–IKKα–p65 scaffolding, cytokine ELISAs with STRAP knockdown in macrophages, and GSK-3β/β-catenin interaction studies with in vivo metastasis model

    PMID:26910283 PMID:27934954

    Open questions at the time
    • Whether STRAP scaffold function in immunity requires the same domains as in TGF-β inhibition not fully mapped
    • No structural model of STRAP immune signaling complexes
  13. 2017 High

    Extension of STRAP's innate immune scaffold function to TLR3 signaling via TBK1–IRF3 interaction and STRAP's disruption of PRC2 assembly to epigenetically activate NOTCH signaling broadened the scope of STRAP from a receptor-proximal scaffold to a chromatin-level regulatory platform.

    Evidence Co-IP and cytokine assays for TLR3/TBK1/IRF3; ChIP-seq showing reduced H3K27me3 at NOTCH gene promoters upon STRAP expression with rescue experiments

    PMID:28651742 PMID:28827371

    Open questions at the time
    • Whether STRAP directly contacts EZH2 or acts through an intermediary not fully resolved
    • In vivo immune phenotype of STRAP loss not established
  14. 2019 High

    Reconstitution experiments showed that STRAP/TTC5 inhibits JMY-mediated actin nucleation at phagophore membranes, identifying STRAP as a negative regulator of autophagy that acts by competing with LC3-dependent JMY activation.

    Evidence In vitro reconstitution with membrane-bound LC3, actin nucleation assay, and co-IP

    PMID:30593260

    Open questions at the time
    • Physiological relevance of STRAP autophagy inhibition in vivo unknown
    • Whether this connects to STRAP's other cytoplasmic functions unaddressed
  15. 2020 High

    Genome-wide eCLIP-seq in mouse embryos and snRNP assembly assays revealed that STRAP directly binds RNA, preferentially targets neurodevelopmental transcripts, regulates U2 snRNP assembly and alternative splicing, and is required for neural tube closure in Xenopus—unifying its SMN complex role with a direct RNA-binding and splicing regulatory function.

    Evidence eCLIP-seq, STRAP-knockout embryoid bodies, U2 snRNP assembly assay, Xenopus morpholino knockdown, RNA-seq splicing analysis

    PMID:33230114

    Open questions at the time
    • Whether STRAP binds RNA through the OB-fold domain not determined
    • Human neurodevelopmental disease association not established
  16. 2020 High

    Identification of CBP as the acetyltransferase and SIRT7 as the deacetylase for STRAP at Lys147/148/156 provided a regulatory PTM code that tunes STRAP's p53 co-activator function, complementing the earlier ATM/Chk2 phosphorylation axis.

    Evidence Mass spectrometry acetylation site mapping, mutagenesis (3KR/3KQ), co-IP for CBP and SIRT7, p53 functional assays

    PMID:32527012

    Open questions at the time
    • How acetylation and phosphorylation are coordinated on STRAP unclear
    • Whether acetylation affects non-p53 functions of STRAP unknown
  17. 2025 High

    The Csde1–Strap complex was shown to couple Bach2 mRNA decay with translation to control plasma cell differentiation, and USP38 was identified as the deubiquitinase stabilizing STRAP to enhance TGF-β/SMAD-dependent atrial fibrosis, revealing tissue-specific post-translational and RNA-level regulatory circuits centered on STRAP.

    Evidence CRISPR screen, RIP, mRNA decay/polysome assays for Csde1–Strap–Bach2; deubiquitination assay and cardiomyocyte-specific mouse genetics for USP38–STRAP

    PMID:40133358 PMID:40514673

    Open questions at the time
    • Whether USP38 regulation of STRAP is relevant outside cardiac fibrosis not tested
    • Structural basis of Csde1–Strap complex unknown
  18. 2026 High

    iNOS-mediated S-nitrosylation of STRAP at Cys152/270 disrupts its interaction with ASK1, activating the ASK1–MKK3–p38 apoptotic axis—demonstrating that the same cysteine residues used for multiple protein interactions are also redox-regulated switches.

    Evidence Biotin switch S-nitrosylation assay, cysteine-to-serine mutagenesis, kinase activity assays, apoptosis assays

    PMID:41519199

    Open questions at the time
    • Whether S-nitrosylation also disrupts STRAP–p53 or STRAP–MPK38 interactions not tested
    • In vivo relevance in inflammatory or ischemic contexts not established

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: how STRAP partitions among its many complexes (SMN, TGF-β receptor, TLR, mitochondria, chromatin) in a cell-type-specific manner; whether high-resolution structures of STRAP bound to its partners will reveal allosteric mechanisms; and whether STRAP mutations cause human developmental or immune disease.
  • No structural model of any STRAP–partner complex
  • No Mendelian disease linked to STRAP mutations
  • Cell-type-specific partitioning mechanism unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0098772 molecular function regulator activity 4 GO:0003723 RNA binding 2 GO:0140110 transcription regulator activity 2
Localization
GO:0005634 nucleus 3 GO:0005829 cytosol 2 GO:0005739 mitochondrion 1
Pathway
R-HSA-162582 Signal Transduction 5 R-HSA-5357801 Programmed Cell Death 4 R-HSA-8953854 Metabolism of RNA 3 R-HSA-168256 Immune System 2 R-HSA-392499 Metabolism of proteins 2 R-HSA-74160 Gene expression (Transcription) 2 R-HSA-9612973 Autophagy 1
Partners
CSDE1GEMIN7GSK3BJMYLARP6SMAD7TBK1TP53
Complex memberships
Csde1-Strap complexSMN complexTAK1-IKKα-p65 signaling complex

Evidence

Reading pass · 34 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 STRAP (serine-threonine kinase receptor-associated protein) was identified as a novel WD40-domain protein that interacts with TGF-β type I receptor (TβR-I) in a yeast two-hybrid system and associates with both functional TβR-I and TβR-II in vivo. Overexpression of STRAP inhibits TGF-β-mediated transcriptional activation and shows synergistic inhibition with Smad7 but not Smad6. Yeast two-hybrid, co-immunoprecipitation, transcriptional reporter assays The Journal of biological chemistry High 9856985
2000 STRAP synergizes specifically with Smad7 (not Smad6) to inhibit TGF-β-induced transcriptional responses by recruiting Smad7 to the activated type I receptor and stabilizing the Smad7-receptor complex, thereby preventing Smad2 and Smad3 access to the receptor. STRAP associates stably with Smad7 but not with a C-terminal deletion mutant of Smad7 that lacks receptor-binding activity. The C terminus of STRAP is required for its phosphorylation in vivo, which depends on TGF-β receptor kinases. Co-immunoprecipitation, transcriptional reporter assays, deletion mutagenesis, in vivo phosphorylation assay Molecular and cellular biology High 10757800
2006 STRAP is localized in both cytoplasm and nucleus, and its stable expression activates the MEK/ERK pathway and downregulates the CDK inhibitor p21(Cip1), resulting in retinoblastoma protein hyperphosphorylation. STRAP-knockout mouse embryonic fibroblasts show enhanced Smad2/3 phosphorylation, TGF-β-mediated transcription, and growth inhibition compared to wild-type cells. Stable overexpression, knockdown by siRNA, western blotting, STRAP-knockout MEFs, subcellular fractionation/localization Cancer research High 16778189
2007 NM23-H1 and STRAP directly interact with the central DNA-binding domain of p53 (residues 113–290). Specific cysteine residues mediate binding: Cys145 of NM23-H1 and Cys152/Cys270 of STRAP bind p53 at Cys176 and Cys135, respectively. NM23-H1 and STRAP potentiate p53 transcriptional activity and p53-induced apoptosis by removing Mdm2 from the p53-Mdm2 complex. Co-immunoprecipitation, mutagenesis, transcriptional reporter assays, apoptosis assays, siRNA knockdown The Journal of biological chemistry High 17916563
2008 ATM kinase directly phosphorylates Strap, prompting its nuclear accumulation by impeding nuclear export. Subsequently, Chk2 kinase phosphorylates Strap to augment protein stability once Strap has attained a nuclear location. These two kinase-dependent events cooperate to regulate Strap during the DNA damage response. In vitro kinase assays, mutational analysis, nuclear/cytoplasmic fractionation, live-cell imaging EMBO reports High 18833288
2008 p49/STRAP (SRFBP1) was identified as a cofactor of serum response factor (SRF) and interacts with NDUFAB1, a subunit of NADH dehydrogenase, via yeast two-hybrid. The two proteins co-localize in the cell. Overexpression of p49/STRAP alters intracellular NAD levels, reduces the NAD/NADH ratio, and induces deacetylation of SRF. Yeast two-hybrid, co-immunoprecipitation, co-localization, NAD/NADH measurement, SRF deacetylation assay BMC cell biology Medium 18230186
2009 Deletion of STRAP from mouse embryonic fibroblasts (MEFs) results in loss of mesenchymal morphology and acquisition of epithelial features, upregulation of E-cadherin and formation of adherens junctions, β-catenin relocalization to the cell membrane, and downregulation of the mesenchymal marker LEF1. Upregulation of WT1 in STRAP-null MEFs drives E-cadherin induction. Stable re-expression of STRAP reverses this phenotype. STRAP-knockout MEFs, gene expression profiling, western blotting, immunofluorescence, stable re-expression Cellular signalling High 19781628
2010 B-MYB is a STRAP-interacting protein; the N-terminal DNA-binding domain and a region (aa 373–468) of B-MYB mediate the interaction. B-MYB enhances STRAP-mediated inhibition of TGF-β signaling by modulating complex formation between the TGF-β receptor and SMAD3 or SMAD7, and stimulates STRAP-mediated p53-induced apoptosis and cell cycle arrest. B-MYB prevents Smad3 nuclear translocation and promotes p53 nuclear translocation. Co-immunoprecipitation, deletion mapping, transcriptional reporter assays, confocal microscopy, apoptosis assays The Journal of biological chemistry High 21148321
2011 STRAP binds GSK3β through its WD40 domains, and STRAP, GSK3β, and Axin form a ternary complex. The intracellular fragment of Notch3 (ICN3) binds GSK3β through its ankyrin repeat domain and also binds STRAP through the ankyrin repeat region. STRAP reduces ubiquitination of ICN3, stabilizing it. Small-molecule GSK3β inhibitors reduce the STRAP-GSK3β interaction. Co-immunoprecipitation, deletion mapping, in vivo ubiquitination assay, small-molecule inhibitor treatment Cell cycle High 21502811
2011 STRAP regulates c-Jun stability by decreasing its ubiquitylation and proteasomal degradation. Loss of STRAP in MEFs reduces phospho-c-Jun and total c-Jun, decreases cyclin D1 expression, and reduces cell growth. Overexpression of STRAP in STRAP-null fibroblasts restores c-Jun expression, and STRAP overexpression decreases ubiquitylation of c-Jun in 293T cells. STRAP-knockout MEFs, proteasome inhibition assay, ubiquitylation assay, stable re-expression, western blotting Biochemical and biophysical research communications High 21397588
2012 Crystal structure of full-length Strap (stress-responsive activator of p300) was solved at 2.05 Å resolution, revealing an atypical six-tetratricopeptide repeat (TPR) protein that also contains an unexpected oligonucleotide/oligosaccharide-binding (OB)-fold domain. This domain organization provides an extended superhelical scaffold for protein-protein and protein-DNA interactions. Both TPR and OB-fold domains localize to chromatin of p53 target genes and exhibit intrinsic regulatory activity necessary for the Strap-dependent p53 response. X-ray crystallography, chromatin immunoprecipitation (ChIP), functional reporter assays Proceedings of the National Academy of Sciences of the United States of America High 22362889
2013 STRAP is tethered to collagen α1(I) and α2(I) mRNAs via interaction with LARP6 (which directly binds the 5' stem-loop of collagen mRNAs) through LARP6's C-terminal domain. Tethered STRAP restrains translation of collagen α2(I) mRNA by interacting with eIF4A. In the absence of STRAP, collagen α2(I) mRNA is unrestrictedly loaded onto polysomes, causing imbalanced synthesis of α1(I) and α2(I) polypeptides, hypermodification of α1(I), and inefficient assembly of the collagen trimer. Supplementing STRAP partially restores these defects. RNA pulldown, polysome profiling, co-immunoprecipitation, siRNA knockdown, rescue experiment, collagen secretion assay Molecular and cellular biology High 23918805
2014 MPK38 (murine protein serine/threonine kinase 38) directly phosphorylates STRAP at Ser188 via direct interaction. The STRAP-MPK38 complex is formed through Cys152 and Cys270 of STRAP and Cys339 and Cys377 of MPK38, suggesting redox-dependent interaction. MPK38-mediated Ser188 phosphorylation converts STRAP from an anti-apoptotic to a pro-apoptotic regulator, modulating ASK1, TGF-β, p53, and PI3K/PDK1 signaling pathways. In vitro kinase assay, mutagenesis, inducible shRNA knockdown, adenoviral delivery in mice, cell death assays Cell cycle High 25485581
2014 Strap localizes to mitochondria where it interacts with ATP synthase. This interaction downregulates mitochondrial ATP production. Under glucose-limiting conditions, mitochondrial Strap sensitizes cancer cells to apoptosis. Strap also augments the apoptotic effects of mitochondrial p53. Subcellular fractionation, co-immunoprecipitation, ATP production assay, apoptosis assays, extracellular ATP rescue Cell death and differentiation High 25168243
2014 STRAP downregulates E-cadherin and p21(Cip1) by abrogating the binding of transcription factor Sp1 to its consensus binding sites on these promoters. STRAP recruits HDAC1 to Sp1 binding sites in the p21(Cip1) promoter. Loss of STRAP stabilizes Sp1 by repressing its ubiquitination in G1 phase, leading to enhanced p21(Cip1) expression and cell cycle arrest. ChIP assay, STRAP-knockout MEFs, siRNA knockdown, luciferase reporter assay, ubiquitination assay, cell cycle analysis Cell cycle High 25483064
2014 p49/STRAP overexpression reduces actin content in cultured cells, results in smaller cell size, and causes malformations in transgenic mice including asymmetric abdominal/thoracic cavities and cardiac morphology changes. p49/STRAP co-localizes with nucleolin in the nucleolus and has a BUD22 domain at its C-terminus. p49/STRAP alters expression of muscle-specific genes including the SRF gene. Transgenic mouse model, GFP co-localization, actin content measurement, gene expression analysis BMC cell biology Medium 25183317
2016 STRAP binds GSK-3β and reduces phosphorylation, ubiquitylation, and degradation of β-catenin by preventing its binding to the destruction complex, thereby activating Wnt/β-catenin signaling and upregulating downstream targets including Cyclin D1, MMP2, MMP9, and β-TrCP. This promotes colorectal cancer invasion and metastasis. Co-immunoprecipitation, siRNA knockdown, western blotting, in vitro invasion assay, in vivo metastasis model, human CRC specimens Oncotarget High 26910283
2016 STRAP acts as a scaffold protein in TLR2/4-mediated innate immune signaling by binding TAK1 and IKKα along with NF-κB subunit p65, enhancing TAK1-IKKα-p65 association, and facilitating p65 phosphorylation and nuclear translocation. STRAP depletion severely impairs IL-6, TNF-α, and IL-1β production in macrophages stimulated with TLR2 or TLR4 agonists. The C-terminal region of STRAP is essential for this activity. STRAP also translocates to the nucleus at later times after LPS stimulation to prolong IL-6 mRNA production. Co-immunoprecipitation, siRNA knockdown, overexpression, cytokine ELISA, nuclear translocation assay, C-terminus deletion Scientific reports High 27934954
2017 STRAP acts as a scaffold protein in TLR3-triggered signaling by interacting with TBK1 and IRF3, enhancing IFN-β production. STRAP knockdown reduces both pro-inflammatory cytokine and IFN production in TLR3 agonist-stimulated macrophages. The C-terminus of STRAP is essential for its function in TLR3-mediated IL-6 and IFN-β production. Co-immunoprecipitation, siRNA knockdown, overexpression, cytokine ELISA, C-terminus deletion Cellular immunology High 28651742
2017 STRAP antagonizes formation of the PRC2 chromatin modifier complex by competitively disrupting the association of PRC2 subunits EZH2 and SUZ12, thereby inhibiting PRC2 assembly and reducing H3K27me3 marks on NOTCH pathway gene promoters. This epigenetically activates NOTCH signaling and maintains cancer stem cell subpopulations in colorectal cancer. Restoring the NOTCH pathway by expressing NICD1 or HES1 in STRAP-depleted cells reverses the CSC phenotype. Co-immunoprecipitation, ChIP-seq, siRNA knockdown, lentiviral rescue, in vitro and in vivo tumor assays Cancer research High 28827371
2018 STRAP interacts with MELK (maternal embryonic leucine zipper kinase) and their association is phosphorylation-dependent. Sanguinarine dephosphorylates STRAP and MELK and disrupts their interaction, triggering Bax-dependent intrinsic apoptosis in colorectal cancer cells. Co-immunoprecipitation, immunofluorescence, in vitro kinase activity assay, western blotting, in vivo orthotopic model BMC cancer Medium 29783958
2018 Strap associates with Csde1 (Cold shock domain protein e1/Unr) in erythroblasts and is the most strongly associated protein with Csde1. Reduced Strap expression alters mRNA and/or protein expression of several Csde1-bound transcripts involved in translational regulation during hypoxia (Hmbs, eIF4g3, Pabpc4), as well as Vim and Elavl1, without altering the overall pool of Csde1-bound transcripts. Co-immunoprecipitation, mass spectrometry, RIP (RNA immunoprecipitation), siRNA knockdown, proteomics PloS one Medium 30138317
2019 During autophagy, TTC5/STRAP binds JMY and antagonizes JMY's actin nucleation activity. LC3 recruits JMY to the phagophore and promotes its actin nucleation activity. An in vitro reconstitution system demonstrated that membrane-bound LC3 is sufficient to recruit JMY and stimulate JMY-mediated actin filament assembly, while STRAP acts as a negative autophagy regulator by competing with this activation. In vitro reconstitution, co-immunoprecipitation, actin nucleation assay Autophagy High 30593260
2020 STRAP was identified as a spliceosome-associated factor. Upon Strap deletion, numerous alternative splicing events occur in mouse embryoid bodies undergoing neuroectoderm-like differentiation. Global mapping by eCLIP-seq in mouse embryos reveals STRAP preferentially targets transcripts for nervous system development and regulates alternative splicing through preferred binding positions. STRAP is involved in the assembly of 17S U2 snRNP proteins. In Xenopus, loss of Strap impedes lineage differentiation, delays neural tube closure, and alters exon skipping. eCLIP-seq, STRAP-knockout mouse embryoid bodies, Xenopus loss-of-function, U2 snRNP assembly assay, RNA-seq splicing analysis Nature communications High 33230114
2020 GDF5 increases expression of STRAP and NME1 in SH-SY5Y neuronal cells. Expression of both STRAP and NME1 is necessary and sufficient for the promotion of neurite growth by GDF5 in SH-SY5Y cells and in cultured midbrain dopaminergic neurons. Proteomics, siRNA knockdown, overexpression, neurite length quantification iScience Medium 32853992
2020 STRAP is acetylated at lysines 147, 148, and 156 by the acetyltransferases CBP. The deacetylase SIRT7 reverses this acetylation. Hypo- or hyperacetylation mutations (3KR or 3KQ) of STRAP influence its activation and stabilization of p53. Following 5-FU treatment, STRAP is mobilized from the cytoplasm to the nucleus and promotes STRAP acetylation. Mass spectrometry identification of acetylation sites, mutagenesis, co-immunoprecipitation, subcellular fractionation, p53 functional assays International journal of molecular sciences High 32527012
2024 circPCNXL2 directly binds STRAP and induces interaction between STRAP and MEK1/2, resulting in activation of ERK/MAPK pathways and tumor promotion in intrahepatic cholangiocarcinoma. RNA pulldown, mass spectrometry, RIP (RNA immunoprecipitation), co-immunoprecipitation, luciferase reporter, xenograft model Molecular cancer Medium 38365721
2025 The deubiquitinase USP38 stabilizes STRAP via deubiquitination, thereby enhancing TGF-β/SMAD signaling and promoting atrial fibrosis in chronic kidney disease-associated atrial fibrillation. STRAP knockdown reverses the pro-fibrotic effects induced by USP38 overexpression. Co-immunoprecipitation, ubiquitination assay, cardiomyocyte-specific knockout/overexpression mice, immunofluorescence, STRAP knockdown rescue Molecular medicine High 40514673
2025 The Csde1-Strap complex binds Bach2 mRNA and couples its decay with translation to control the magnitude and duration of Bach2 protein expression during B cell differentiation. In the absence of Csde1 or Strap, Bach2 translation is decoupled from mRNA decay, leading to elevated and prolonged Bach2 protein expression and impaired plasma cell differentiation. RNA interactome capture, CRISPR/Cas9 screening, RIP, co-immunoprecipitation, mRNA decay assays, polysome profiling Nature communications High 40133358
2026 iNOS mediates S-nitrosylation of STRAP specifically at Cys152 and Cys270. S-nitrosylation disrupts the STRAP-ASK1 interaction, increases ASK1 activity, activates the MKK3-p38 pathway, and enhances hydrogen peroxide-induced apoptosis. Mutation of Cys152/270 to serine abolishes the STRAP-ASK1 interaction and constitutively activates the ASK1-MKK3-p38 pathway. STRAP specifically interacts with iNOS but not eNOS or nNOS. S-nitrosylation assay (biotin switch), co-immunoprecipitation, mutagenesis, kinase activity assay, apoptosis assays, iNOS overexpression The Journal of biological chemistry High 41519199
2005 Unrip (STRAP) is integrated into the SMN complex via a stable interaction with Gemin7. It is also found in a mutually exclusive complex with Unr. Unrip is absent from nuclear gems/Cajal bodies and localizes predominantly to the cytoplasm. RNAi-induced reduction of Unrip leads to enhanced accumulation of SMN in the nucleus and increased formation of nuclear gems/Cajal bodies. Co-immunoprecipitation, RNAi knockdown, immunofluorescence localization, biochemical fractionation Human molecular genetics High 16159890
2005 Unrip (STRAP) directly interacts with Gemin6 and Gemin7 within the SMN complex, also binds a subset of Sm proteins, and unrip-containing SMN complexes are necessary and sufficient to mediate the assembly of spliceosomal snRNPs in vitro. Biochemical reconstitution of snRNP assembly, co-immunoprecipitation, pulldown assays FEBS letters High 15848170
2009 p49/STRAP interacts with the β-sandwich domain of Hsp70. It reduces ATP-hydrolytic activity of Hsp70 stimulated by Hsp40 and inhibits the refolding activity of the Hsp70/Hsp40 chaperone system, functioning as a co-chaperone. Co-immunoprecipitation, ATPase assay, protein refolding assay, domain mapping Biochemical and biophysical research communications Medium 19751705
2022 STRAP overexpression in neuroblastoma cell lines activates focal adhesion kinase (FAK) downstream targets as measured by kinomic peptide microarray. FAK inhibitor treatment selectively decreased growth of STRAP-overexpressing neuroblastoma cells compared to controls, placing STRAP upstream of FAK in a signaling pathway. Kinomic peptide microarray (PamChip), stable overexpression, FAK inhibitor (PF-573,228) treatment, proliferation assay Journal of pediatric surgery Medium 35272839

Source papers

Stage 0 corpus · 85 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 Suspension trapping (STrap) sample preparation method for bottom-up proteomics analysis. Proteomics 342 24678027
2018 S-Trap, an Ultrafast Sample-Preparation Approach for Shotgun Proteomics. Journal of proteome research 266 30114372
2001 STRAP: editor for STRuctural Alignments of Proteins. Bioinformatics (Oxford, England) 191 11301311
2000 STRAP and Smad7 synergize in the inhibition of transforming growth factor beta signaling. Molecular and cellular biology 148 10757800
2018 Comparison of In-Solution, FASP, and S-Trap Based Digestion Methods for Bottom-Up Proteomic Studies. Journal of proteome research 141 29754492
1998 Identification of STRAP, a novel WD domain protein in transforming growth factor-beta signaling. The Journal of biological chemistry 104 9856985
2019 Suspension Trapping (S-Trap) Is Compatible with Typical Protein Extraction Buffers and Detergents for Bottom-Up Proteomics. Journal of proteome research 86 30761899
2007 NM23-H1 tumor suppressor and its interacting partner STRAP activate p53 function. The Journal of biological chemistry 82 17916563
2017 STRAP Promotes Stemness of Human Colorectal Cancer via Epigenetic Regulation of the NOTCH Pathway. Cancer research 75 28827371
2005 Unrip, a factor implicated in cap-independent translation, associates with the cytosolic SMN complex and influences its intracellular localization. Human molecular genetics 70 16159890
2006 Oncogenic function of a novel WD-domain protein, STRAP, in human carcinogenesis. Cancer research 67 16778189
2005 Unrip is a component of SMN complexes active in snRNP assembly. FEBS letters 66 15848170
2000 Molecular cloning and characterization of human MAWD, a novel protein containing WD-40 repeats frequently overexpressed in breast cancer. Cancer research 61 10646843
2023 Stratification of biological therapies by pathobiology in biologic-naive patients with rheumatoid arthritis (STRAP and STRAP-EU): two parallel, open-label, biopsy-driven, randomised trials. The Lancet. Rheumatology 42 38251532
2018 Sanguinarine triggers intrinsic apoptosis to suppress colorectal cancer growth through disassociation between STRAP and MELK. BMC cancer 41 29783958
2013 Serine-threonine kinase receptor-associated protein (STRAP) regulates translation of type I collagen mRNAs. Molecular and cellular biology 40 23918805
2023 Squalene epoxidase promotes hepatocellular carcinoma development by activating STRAP transcription and TGF-β/SMAD signalling. British journal of pharmacology 36 36581319
2019 Self-Assembled STrap for Global Proteomics and Salivary Biomarker Discovery. Journal of proteome research 36 30848925
2004 DNA binding provides a molecular strap activating the adenovirus proteinase. Molecular & cellular proteomics : MCP 30 15220401
2016 Novel role of STRAP in progression and metastasis of colorectal cancer through Wnt/β-catenin signaling. Oncotarget 27 26910283
2011 TGF-beta-dependent and -independent roles of STRAP in cancer. Frontiers in bioscience (Landmark edition) 27 21196161
2011 Role of STRAP in regulating GSK3β function and Notch3 stabilization. Cell cycle (Georgetown, Tex.) 27 21502811
2004 STRAP is a strong predictive marker of adjuvant chemotherapy benefit in colorectal cancer. Neoplasia (New York, N.Y.) 27 15720808
2014 Investigation of in vitro anticancer and DNA strap interactions in live cells using carboplatin type Cu(II) and Zn(II) metalloinsertors. European journal of medicinal chemistry 26 25128669
2024 CircPCNXL2 promotes tumor growth and metastasis by interacting with STRAP to regulate ERK signaling in intrahepatic cholangiocarcinoma. Molecular cancer 24 38365721
2014 Oncogenic STRAP functions as a novel negative regulator of E-cadherin and p21(Cip1) by modulating the transcription factor Sp1. Cell cycle (Georgetown, Tex.) 23 25483064
2014 A crucial role for the phosphorylation of STRAP at Ser(188) by MPK38 in STRAP-dependent cell death through ASK1, TGF-β, p53, and PI3K/PDK1 signaling pathways. Cell cycle (Georgetown, Tex.) 23 25485581
2015 Calix[4]pyrroles with Shortest Possible Strap: Exclusively Selective toward Fluoride Ion. Organic letters 18 26313641
2014 Overexpression of p49/STRAP alters cellular cytoskeletal structure and gross anatomy in mice. BMC cell biology 18 25183317
2013 MAWBP and MAWD inhibit proliferation and invasion in gastric cancer. World journal of gastroenterology 18 23687415
2008 Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein. BMC cell biology 18 18230186
2008 ATM and Chk2 kinase target the p53 cofactor Strap. EMBO reports 18 18833288
2003 KISS for STRAP: user extensions for a protein alignment editor. Bioinformatics (Oxford, England) 18 14668241
2021 Carbaporphyrin Dimers That Bear a Rigid Naphthalene Motif as an Internal Strap. Organic letters 17 33577339
2020 STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells. Nature communications 17 33230114
2018 Dual Roles of Serine-Threonine Kinase Receptor-Associated Protein (STRAP) in Redox-Sensitive Signaling Pathways Related to Cancer Development. Oxidative medicine and cellular longevity 17 29849900
2012 The p53 cofactor Strap exhibits an unexpected TPR motif and oligonucleotide-binding (OB)-fold structure. Proceedings of the National Academy of Sciences of the United States of America 17 22362889
2015 C-STrap Sample Preparation Method--In-Situ Cysteinyl Peptide Capture for Bottom-Up Proteomics Analysis in the STrap Format. PloS one 16 26407052
2009 Developmental morphology of strap-shaped gametophytes of Colysis decurrens: a new look at meristem development and function in fern gametophytes. Annals of botany 15 19812067
2020 STRAP and NME1 Mediate the Neurite Growth-Promoting Effects of the Neurotrophic Factor GDF5. iScience 14 32853992
2010 B-MYB positively regulates serine-threonine kinase receptor-associated protein (STRAP) activity through direct interaction. The Journal of biological chemistry 14 21148321
2009 Serine threonine receptor-associated protein (STRAP) plays a role in the maintenance of mesenchymal morphology. Cellular signalling 14 19781628
2021 Comparison of protein characterization using In solution and S-Trap digestion methods for proteomics. Biochemical and biophysical research communications 13 34922203
2020 SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability. International journal of molecular sciences 13 32527012
2016 Does p49/STRAP, a SRF-binding protein (SRFBP1), modulate cardiac mitochondrial function in aging? Experimental gerontology 13 27337995
2014 Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase. Cell death and differentiation 13 25168243
2005 Structural interpretation of mutations and SNPs using STRAP-NT. Protein science : a publication of the Protein Society 13 16322575
2023 Suspension TRAPping Filter (sTRAP) Sample Preparation for Quantitative Proteomics in the Low µg Input Range Using a Plasmid DNA Micro-Spin Column: Analysis of the Hippocampus from the 5xFAD Alzheimer's Disease Mouse Model. Cells 11 37174641
2018 Strap associates with Csde1 and affects expression of select Csde1-bound transcripts. PloS one 11 30138317
2018 Oncogenic roles of serine-threonine kinase receptor-associated protein (STRAP) in osteosarcoma. Cancer chemotherapy and pharmacology 11 30276452
2021 Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Knockout Decreases the Malignant Phenotype in Neuroblastoma Cell Lines. Cancers 10 34206917
2021 Folding in Place: Design of β-Strap Motifs to Stabilize the Folding of Hairpins with Long Loops. The Journal of organic chemistry 10 34499510
2019 Regulation of JMY's actin nucleation activity by TTC5/STRAP and LC3 during autophagy. Autophagy 10 30593260
2018 Oncogenic STRAP Supports Hepatocellular Carcinoma Growth by Enhancing Wnt/β-Catenin Signaling. Molecular cancer research : MCR 10 30257989
2016 STRAP Acts as a Scaffolding Protein in Controlling the TLR2/4 Signaling Pathway. Scientific reports 10 27934954
2020 S-Trap Eliminates Cell Culture Media Polymeric Surfactants for Effective Proteomic Analysis of Mammalian Cell Bioreactor Supernatants. Journal of proteome research 9 32207952
2020 Developing a mass spectrometry-based assay for the ovarian cancer biomarker CA125 (MUC16) using suspension trapping (STrap). Analytical and bioanalytical chemistry 9 32215689
2017 STRAP positively regulates TLR3-triggered signaling pathway. Cellular immunology 9 28651742
2015 Mitogen-activated protein kinase activator with WD40 repeats (MAWD) and MAWD-binding protein induce cell differentiation in gastric cancer. BMC cancer 9 26373288
2011 STRAP regulates c-Jun ubiquitin-mediated proteolysis and cellular proliferation. Biochemical and biophysical research communications 9 21397588
2025 Deep molecular profiling of synovial biopsies in the STRAP trial identifies signatures predictive of treatment response to biologic therapies in rheumatoid arthritis. Nature communications 8 40603860
2013 STRAP PTM: Software Tool for Rapid Annotation and Differential Comparison of Protein Post-Translational Modifications. Current protocols in bioinformatics 8 25422678
1999 Influence of girth strap tensions on athletic performance of racehorses. Equine veterinary journal. Supplement 8 10659222
2023 Comparison of SPEED, S-Trap, and In-Solution-Based Sample Preparation Methods for Mass Spectrometry in Kidney Tissue and Plasma. International journal of molecular sciences 7 37047281
2017 Downregulation of STRAP promotes tumor growth and metastasis in hepatocellular carcinoma via reducing PTEN level. IUBMB life 7 29283497
2006 HotSwap for bioinformatics: a STRAP tutorial. BMC bioinformatics 7 16469097
2008 Strap: a versatile transcription co-factor. Cell cycle (Georgetown, Tex.) 6 18719389
2006 Identification and characterization of p49/STRAP as a novel GLUT4-binding protein. Biochemical and biophysical research communications 6 16647043
2025 Ubiquitin-specific protease 38 modulates atrial fibrillation susceptibility in chronic kidney disease via STRAP stabilization and activation of TGF-β/SMAD signaling. Molecular medicine (Cambridge, Mass.) 5 40514673
2022 Serine-Threonine Kinase Receptor Associate Protein (STRAP) confers an aggressive phenotype in neuroblastoma via regulation of Focal Adhesion Kinase (FAK). Journal of pediatric surgery 5 35272839
2016 X-radiation enhances the collagen type I strap formation and migration potentials of colon cancer cells. Oncotarget 5 27655687
2015 Keratinized strap cells: a rare cytological atypia resembles Anitschkow cells, in human oral neoplasm. International journal of clinical oncology 5 26160758
2021 Characterizing the Host Coral Proteome of Platygyra carnosa Using Suspension Trapping (S-Trap). Journal of proteome research 4 33630606
2017 p49/STRAP, a Serum Response Factor Binding Protein (SRFBP1), Is Involved in the Redistribution of Cytoskeletal F-Actin Proteins during Glucose Deprivation. The journal of nutrition, health & aging 4 29188873
2009 Interaction of Hsp70 with p49/STRAP, a serum response factor binding protein. Biochemical and biophysical research communications 4 19751705
2024 Protocol for feeding strategy and proteomics analysis of zebrafish Danio rerio using S-trap and iTRAQ techniques. STAR protocols 3 39661508
2023 "Out of the blue": A qualitative study exploring the experiences of women and next of kin receiving unexpected results from BRA-STRAP research gene panel testing. Journal of genetic counseling 3 37864663
2020 STRAP reduces endoplasmic reticulum stress and apoptosis in cardiomyocytes and attenuates myocardial ischemia-reperfusion injury by activating PI3K/PDK1/Akt signaling pathway. European review for medical and pharmacological sciences 3 32373981
2013 Padded self-adhesive strap immobilization following newborn bladder exstrophy closure: the Utah straps. The Journal of urology 3 23810641
2025 A Csde1-Strap complex regulates plasma cell differentiation by coupling mRNA translation and decay. Nature communications 2 40133358
2024 STRAP upregulates antiviral innate immunity against PRV by targeting TBK1. Virology journal 1 39182136
2024 STRAP Knockdown Inhibits Migration and Growth of Non-Small Cell Lung Cancer. Bulletin of experimental biology and medicine 1 39455498
2026 S-nitrosylation of the scaffold protein STRAP enhances oxidative stress-induced apoptosis. The Journal of biological chemistry 0 41519199
2025 The Role of Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Signaling in Cancer. Cells 0 40558481
2012 StRAP: an integrated resource for profiling high-throughput cancer genomic data from stress response studies. PloS one 0 23284744