Affinage

RAB7A

Ras-related protein Rab-7a · UniProt P51149

Length
207 aa
Mass
23.5 kDa
Annotated
2026-06-10
100 papers in source corpus 49 papers cited in narrative 49 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAB7A is a small GTPase that acts as the master organizer of the endolysosomal system, cycling between GTP-bound (active) and GDP-bound (inactive) states to control late endosome maturation, motility, and fusion with lysosomes (PMID:10679007). Active GTP-RAB7A recruits a diverse set of effectors from the cytosol to late endosomal membranes — including the RING-finger protein Rabring7 (PMID:12972561), the RH-domain proteins Rubicon and PLEKHM1 (PMID:20943950), WDR91 (PMID:28860274), LKB1 (PMID:25180605), and retromer (PMID:29777037) — to govern cargo degradation, endosome-to-Golgi retrieval, and autophagic flux, while motor-adaptor coupling through RILP and dynein directs microtubule-based retrograde transport (PMID:16965270, PMID:35474277). Its activation cycle is set by upstream regulators: the Mon1-Ccz1-C18orf8/RMC1 GEF complex loads GTP to license RAB7A function including NPC1-dependent lysosomal cholesterol export (PMID:33144569, PMID:34432599), whereas GAPs TBC1D5, Armus/TBC1D2, and TBC1D15 drive GTP hydrolysis to inactivate RAB7A and restrict its membrane domain (PMID:27793976, PMID:29364868, PMID:29158324). RAB7A also coordinates membrane contact sites, directly binding the ER tether PDZD8 and promoting mitochondria-lysosome contacts whose untethering requires FIS1-recruited TBC1D15-driven GTP hydrolysis, with these contacts marking sites of mitochondrial fission (PMID:29364868, PMID:34552186). A layer of post-translational control tunes effector choice and activity: TBK1 (and IKKε) phosphorylate RAB7A at Ser72 to switch effector preference from the inhibitory Rubicon to the activating Pacer, enabling Parkin-dependent mitophagy and ATG9A recruitment (PMID:30627666, PMID:38728007), while Src-mediated Tyr183 phosphorylation impairs RILP binding and slows EGFR degradation (PMID:28336235); succinylation at Lys31 (reversed by SIRT5) (PMID:38837686) and ubiquitination (reversed by USP4) (PMID:39663592) further regulate RAB7A activity and autophagic clearance. In neurons RAB7A marks retrograde carriers transporting neurotrophins and their receptors and maintains axonal and dendritic cargo degradation (PMID:17046692, PMID:29907658), and CMT2B-causing missense mutations hyperactivate RAB7A — elevating GTP loading and slowing hydrolysis — to perturb NGF/TrkA and EGFR trafficking and signaling and to drive neurodegeneration (PMID:18272684, PMID:23616551).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 2000 High

    Established RAB7A as the GTPase controlling late endosome/lysosome fusion and maintenance of the perinuclear lysosomal compartment, defining its core cellular role.

    Evidence Dominant-negative and constitutively active EGFP-Rab7 mutants in HeLa cells scored against lysosomal morphology, acidification, and cargo access

    PMID:10679007

    Open questions at the time
    • Did not identify the effectors mediating fusion
    • Upstream activation machinery unknown
  2. 2003 Medium

    Connected RAB7A to phosphoinositide signaling and placed it downstream of Rab5 in the maturation cascade, showing nucleotide cycling drives effector engagement on late endosomes.

    Evidence Co-IP and PI3K activity assays linking hVPS34/p150 to Rab7; genetic epistasis with dominant-negative Rab5/Rab7 on phagosomes; identification of the GTP-specific effector Rabring7

    PMID:12640132 PMID:12972561 PMID:14617358

    Open questions at the time
    • Direct GEF/GAP identities not yet defined
    • Causal direction between PI(3)P and Rab7 activity unresolved at this stage
  3. 2003 Medium

    Extended RAB7A function beyond canonical degradation to virus entry and to cell-autonomous nutrient transporter clearance/apoptosis, broadening its physiological reach.

    Evidence Dominant-negative Rab7 blocking influenza late-endosomal entry; loss-of-function effects on surface nutrient transporters and survival in growth-factor-deprived cells

    PMID:12713661 PMID:14536059

    Open questions at the time
    • Effectors mediating these specialized roles not defined
    • Mechanism of transporter retention versus degradation unresolved
  4. 2006 High

    Defined RAB7A as the marker of retrograde axonal carriers and stage-specific organelle transport, showing it couples cargo identity to dynein/RILP-based motility.

    Evidence Biochemical purification of retrograde neurotrophin vesicles identifying Rab7 by MS, plus dominant-negative epistasis; colocalization across melanosome maturation stages

    PMID:16965270 PMID:17046692

    Open questions at the time
    • Quantitative contribution of RAB7A versus other GTPases to transport unclear
    • How Rab7 selects specific cargo pools unknown
  5. 2008 High

    Established the molecular basis of CMT2B disease as RAB7A hyperactivation — elevated nucleotide exchange and slowed hydrolysis producing a predominantly GTP-bound mutant that retains effector binding.

    Evidence In vitro GTP hydrolysis and exchange assays, cellular GTP-bound fraction measurement, RILP binding, and siRNA rescue across L129F/K157N/V162M mutants

    PMID:18272684

    Open questions at the time
    • How hyperactivation translates to neuron-specific degeneration not addressed here
    • Effector rewiring downstream of hyperactivation not mapped
  6. 2010 Medium

    Linked CMT2B RAB7A hyperactivation to neuronal phenotypes and identified the RH-domain effectors Rubicon/PLEKHM1, expanding the effector repertoire and tying gain-of-function to neurite defects.

    Evidence Neurite outgrowth assays in PC12/Neuro2A with CMT2B and Q67L mutants; GST pull-down and co-IP defining the RH-domain Rab7 interaction

    PMID:20464402 PMID:20943950

    Open questions at the time
    • Causal chain from effector binding to outgrowth not fully resolved
    • Whether RH-domain effectors mediate the CMT2B phenotype untested
  7. 2012 Medium

    Showed CMT2B mutants distort growth-factor receptor trafficking and signaling, slowing TrkA/EGFR degradation while paradoxically reducing nuclear signal delivery, providing a signaling mechanism for disease.

    Evidence Co-IP, phospho-signaling Westerns, nuclear fractionation, and transcriptional reporters in PC12 cells and multiple lines tracking TrkA, EGFR, Erk1/2, p38, and Elk-1

    PMID:21151572 PMID:23188822

    Open questions at the time
    • Single-lab signaling readouts
    • Relative contribution of TrkA versus EGFR defects to neuropathy unresolved
  8. 2012 High

    Connected RAB7A to the intermediate filament cytoskeleton, identifying direct binding to vimentin and peripherin and a role in filament solubility, hinting at the neuronal specificity of CMT2B.

    Evidence Yeast two-hybrid, co-IP, and pull-downs with recombinant proteins; solubility fractionation with wild-type and CMT2B mutants

    PMID:23179371 PMID:23458836

    Open questions at the time
    • Mechanism linking filament solubility to endosomal function unclear
    • Whether filament binding is GTP-dependent not established
  9. 2013 Medium

    Tested CMT2B disease mechanism in vivo and in primary neurons, with one model supporting gain-of-function signaling/transport defects and another arguing for partial loss of function, exposing a mechanistic divergence.

    Evidence Live axonal transport imaging and TrkA/NGF assays in rat DRG neurons; quantitative imaging, electrophysiology, and rescue in Drosophila neurons

    PMID:23616551 PMID:24327558

    Open questions at the time
    • Gain- versus loss-of-function interpretation unreconciled across systems
    • Species and assay differences may confound the comparison
  10. 2014 Medium

    Identified additional GTP-specific effectors and cargo-degradation roles, including LKB1-mediated NRP-1 degradation, embedding RAB7A in angiogenesis control.

    Evidence GTP- versus GDP-specific co-IP of LKB1 and siRNA rescue of NRP-1 trafficking

    PMID:25180605

    Open questions at the time
    • Single-lab effector assignment
    • Generalizability beyond NRP-1 untested
  11. 2016 High

    Resolved the lipid logic of RAB7A inactivation and effector scaffolding, showing Vps34-derived PI(3)P recruits the GAP Armus to switch off Rab7, and that adaptor complexes link Rab7-lysosomes to microtubules for cell-type functions like bone resorption.

    Evidence Vps34-KO MEFs with Rab7-GTP pull-downs and lipid-binding assays; co-IP and conditional Plekhm1-KO mice with lysosome positioning and bone resorption readouts; siRNA effects on migration and integrin/Rac1 signaling

    PMID:27777970 PMID:27793976 PMID:27888097

    Open questions at the time
    • How PI(3)P spatially restricts Armus to specific endosomes incompletely defined
    • Coordination of GEF and GAP lipid cues unresolved
  12. 2017 High

    Defined a multi-layered control of the RAB7A cycle: tyrosine phosphorylation by Src tuning effector binding, retromer/TBC1D5-dependent restriction of the active Rab7 domain, and WDR91 coupling Rab switching to PI(3)P down-regulation.

    Evidence In vitro Src kinase assay with Y183F mutagenesis; TBC1D5/retromer-KO cells with FRAP and organelle markers; WDR91 reciprocal co-IP, knockout mice, and binding-mutant rescue

    PMID:28222213 PMID:28336235 PMID:28415797 PMID:28716909 PMID:28860274 PMID:29158324 PMID:29777037

    Open questions at the time
    • Integration of multiple PTMs and GAPs onto a single Rab7 pool unresolved
    • Crosstalk between Src, retromer, and lipid cues not jointly tested
  13. 2018 High

    Established the Ser72 phosphoswitch in mitophagy and revealed RAB7A as a regulator of membrane contact sites, controlling mitochondria-lysosome tethering and mitochondrial fission.

    Evidence Phosphoproteomics and in vitro TBK1 kinase assay with S72E/S72A mutants and mitophagy/ATG9A readouts; multi-modal imaging of mitochondria-lysosome contacts with TBC1D15/FIS1 manipulation

    PMID:29364868 PMID:29907658 PMID:30627666

    Open questions at the time
    • Which GEF acts on phospho-Rab7 at damaged mitochondria not fully defined at this stage
    • How contact-site Rab7 is spatially distinguished from degradative Rab7 unclear
  14. 2019 Medium

    Dissected phosphoinositide-driven Rab7 cycling on/off membranes and showed the Ser72 phosphoswitch also governs innate immune (STING) and ciliary outcomes, broadening its regulatory reach.

    Evidence Acute PI4P-to-PI(4,5)P2 conversion and PI4K2A KO with live Rab7/PLEKHM1 imaging; PIPKIγi5 co-IP and KD; phosphomimetic Rab7 effects on STING/IRF3 targets; siRNA effects on ciliogenesis and ectocytosis

    PMID:31368593 PMID:31619485 PMID:31662325 PMID:31908013

    Open questions at the time
    • How distinct phosphoinositides are coordinated across endosome maturation incompletely resolved
    • Single-lab readouts for STING and cilia roles
  15. 2020 High

    Identified the trimeric Mon1-Ccz1-C18orf8 GEF as the activator licensing Rab7-NPC1-dependent lysosomal cholesterol export, and showed CLN3 organizes Rab7-effector interactions, anchoring Rab7 in lysosomal storage disease biology.

    Evidence Genome-wide CRISPR screen, GEF subunit KOs with active-Rab7 rescue, and Rab7-NPC1 co-IP; CLN3-KO and disease-mutant co-IP with retromer/PLEKHM1 trafficking assays

    PMID:32034082 PMID:33144569

    Open questions at the time
    • Stoichiometry and assembly of the GEF complex on membranes not fully resolved
    • How CLN3 mechanistically promotes effector binding unclear
  16. 2021 Medium

    Defined RMC1/C5orf51 as a MON1-CCZ1 component stabilizing GDP-Rab7, solved the structural basis of GTP-dependent PDZD8 tethering, and showed Parkin ubiquitinates and degrades Rab7 and its GEF to limit mitophagy.

    Evidence miniTurbo proximity proteomics and co-IP with GDP-locked Rab7 plus C5orf51 KO; crystal structure and ITC of PDZD8-CC:GTP-Rab7; oocyte Rab7Q67L/ML098 rescue with ubiquitin-proteasome analysis

    PMID:34229552 PMID:34432599 PMID:34552186

    Open questions at the time
    • How GEF complex composition is regulated dynamically unresolved
    • Physiological balance between Parkin activation and degradation of Rab7 unclear
  17. 2024 High

    Resolved the structural and functional logic of the Ser72 phosphoswitch (Rubicon-to-Pacer exchange), added succinylation/ubiquitination as activity-tuning PTMs, linked Rab7 to mTORC1 and ALS/FTD, and showed viral hijacking of Rab7 GAPs to drive hyperactivation.

    Evidence Rubicon:Rab7 crystal structure with in vitro TBK1 phosphorylation, Pacer KO and phosphate-binding mutant rescue; SIRT5 desuccinylation (K31) and USP4 deubiquitination assays with in vivo models; lysosomal TBK1-Rab7-mTORC1 axis and TBK1 E696K mutant; SARS-CoV-2 ORF3a-Vps39 sequestration of TBC1D5

    PMID:38448435 PMID:38728007 PMID:38837686 PMID:39103493 PMID:39663592

    Open questions at the time
    • How multiple PTMs are integrated on the same Rab7 molecule in time unresolved
    • In vivo relevance of the mTORC1 and STING branches to human disease incompletely established

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how the many parallel inputs — distinct GEF/GAP modules, phosphoinositide cues, and the Ser72/Tyr183/Lys31/ubiquitin post-translational marks — are spatially and temporally integrated on individual Rab7 pools to select specific effectors and membrane outcomes, and how this integration is corrupted in CMT2B and other diseases.
  • No unified model coordinating PTMs, GEFs, GAPs, and lipids on a single Rab7 domain
  • Gain- versus loss-of-function in CMT2B unreconciled across model systems
  • Effector-selection rules across the many specialized Rab7 functions undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 5 GO:0003924 GTPase activity 4
Localization
GO:0005764 lysosome 4 GO:0005739 mitochondrion 2 GO:0005783 endoplasmic reticulum 2 GO:0005829 cytosol 2
Pathway
R-HSA-9609507 Protein localization 4 R-HSA-9612973 Autophagy 4 R-HSA-1430728 Metabolism 3 R-HSA-1643685 Disease 3 R-HSA-5653656 Vesicle-mediated transport 3
Partners
NPC1PDZD8PLEKHM1RILPRUBICONTBC1D15TBC1D5WDR91

Evidence

Reading pass · 49 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 RAB7A (Rab7) controls aggregation and fusion of late endocytic structures/lysosomes and is essential for maintenance of the perinuclear lysosome compartment. Dominant-negative Rab7 mutants (T22N, N125I) caused lysosome dispersal, loss of acidification, and inaccessibility to endocytosed LDL, while constitutively active Rab7Q67L enhanced perinuclear lysosome aggregation and fusion. Transient expression of EGFP-tagged wild-type and mutant Rab7 in HeLa cells; colocalization with cathepsin D, LAMP1, LysoTracker Red, transferrin receptor, CI-M6PR, TGN-38 Molecular biology of the cell High 10679007
2003 hVPS34 (class III PI3K) and its adaptor p150 are direct Rab7 interacting partners that colocalize on late endosomes; hVPS34 kinase activity is dependent on nucleotide cycling of Rab7, and Rab7 expression modulates total cellular PI(3)P levels. Co-immunoprecipitation, colocalization by fluorescence microscopy, PI3K activity assays with dominant-negative and constitutively active Rab7 Traffic (Copenhagen, Denmark) Medium 14617358
2003 Rab5 is required upstream of Rab7 recruitment to phagosomes; however, PI3K is required for Rab5 dissociation from phagosomes, and Rab7 recruitment and activation are insufficient alone to drive phagosome-lysosome fusion (PI3K-dependent effectors beyond Rab7 are required). Genetic epistasis using dominant-negative Rab5 and Rab7 in professional and engineered phagocytes; PI3K inhibition (wortmannin); FRAP for Rab5 activity; measurement of RILP binding as Rab7 activity readout Molecular and cellular biology High 12640132
2003 Rab7 is required for the late endosomal step of influenza virus entry; dominant-negative Rab7 blocks infection by influenza (H1N1 and H3N2) but not by Semliki Forest virus or vesicular stomatitis virus, demonstrating a virus-specific requirement for late endosome function. Dominant-negative Rab5 and Rab7 expression in HeLa cells; viral ribonucleoprotein colocalization time-course; conformation-specific hemagglutinin antibody assays Traffic (Copenhagen, Denmark) Medium 12713661
2003 Rabring7, a RING-finger protein, is a Rab7 effector that specifically binds the GTP-bound form of Rab7 at its N-terminal portion. Rabring7 is recruited from cytosol to late endosomes/lysosomes by active Rab7, and its overexpression causes perinuclear lysosome aggregation and affects EGF degradation. CytoTrap two-hybrid screen; GST pull-down; co-immunoprecipitation; subcellular localization by fluorescence microscopy; EGF degradation assay Molecular biology of the cell Medium 12972561
2003 Rab7 functions as a proapoptotic regulator by limiting cell-autonomous nutrient (glucose and amino acid) transporter expression. Blocking Rab7 in growth-factor-deprived cells prevents clearance of nutrient transporters from the cell surface, sustaining mitochondrial membrane potential and growth-factor-independent survival; dominant-negative Rab7 cooperated with E1A for transformation of p53-/- MEFs. Dominant-negative Rab7 expression; nutrient transporter surface levels; mitochondrial membrane potential measurement; transformation assay in MEFs Developmental cell Medium 14536059
2006 Rab7 controls retrograde axonal transport by marking a specific pool of carriers that transport neurotrophins (BDNF), their receptors (TrkB, p75NTR), and tetanus neurotoxin fragment. Rab5 is essential for an early sorting step but is absent from axonally transported vesicles. Purification of retrogradely transported vesicles using paramagnetic bead-conjugated tetanus neurotoxin fragment; identification of Rab7 by MS; functional validation with Rab5/Rab7 dominant-negatives Neuron High 17046692
2006 Rab7 associates with early and intermediate stage melanosomes and controls microtubule-mediated (dynein/RILP-dependent) transport of these early melanosomes, while mature melanosomes lose Rab7 and instead use Rab27a-myosin Va for actin-dependent transport. Immunofluorescence colocalization of Rab7 and Rab27a with melanosome stages; functional analysis of motor protein recruitment Pigment cell research Medium 16965270
2008 CMT2B-associated Rab7 mutant proteins (L129F, K157N, V162M) exhibit higher nucleotide exchange rates and slower GTP hydrolysis than wild-type Rab7, resulting in 82-89% GTP-bound form in cells vs. 23% for wild type. These mutants bind the Rab7 effector RILP and can rescue Rab7 function after silencing, demonstrating gain-of-function through hyperactivation. In vitro GTP hydrolysis assays; nucleotide exchange rate measurements; GTP-bound fraction determination in HeLa cells; RILP binding assay; siRNA rescue experiments The Journal of neuroscience High 18272684
2010 Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel C-terminal RH domain that directly and specifically interacts with Rab7. This interaction is critical for their regulatory functions. Rubicon, but not PLEKHM1, also simultaneously binds PI3-kinase to regulate trafficking. Database-based homology identification; GST pull-down; co-immunoprecipitation; dominant-negative Rab7 epistasis; pathway readouts for endocytic and autophagic flux Molecular biology of the cell Medium 20943950
2010 CMT2B-associated Rab7 mutants inhibit neurite outgrowth in PC12 and Neuro2A cells, as demonstrated by reduced GAP43 upregulation and NeuN expression. The inhibition phenocopies constitutively active Rab7Q67L, consistent with gain-of-function hyperactivation. Expression of CMT2B Rab7 mutants and constitutively active Rab7 in PC12 and Neuro2A cells; neurite outgrowth measurement; GAP43 and NeuN biochemical quantification Acta neuropathologica Medium 20464402
2010 CMT2B Rab7 mutants enhance NGF-stimulated TrkA phosphorylation, increase Erk1/2 signaling from endosomes, and reduce nuclear shuttling of phospho-Erk1/2, thereby decreasing downstream nuclear transcription (MAPK phosphatase expression) and explaining inhibition of neurite outgrowth. Rab7 CMT2B mutant expression in PC12 cells; TrkA co-immunoprecipitation; phosphorylation assays for TrkA, Akt, Erk1/2; nuclear fractionation PloS one Medium 21151572
2012 CMT2B Rab7 mutants delay EGF trafficking to LAMP1-positive late endosomes/lysosomes, slow EGFR degradation, enhance and prolong EGFR signaling and p38/ERK1/2 phosphorylation, but reduce nuclear translocation of p38/ERK1/2, thereby decreasing downstream nuclear activation of Elk-1 and immediate early genes. EGF trafficking assays in three cell lines; EGFR degradation assays; phospho-signaling Western blots; nuclear fractionation; Elk-1 transcriptional reporter assays The Journal of biological chemistry Medium 23188822
2012 RAB7A directly interacts with vimentin (identified by yeast two-hybrid and confirmed by co-immunoprecipitation and pull-down with recombinant proteins). Overexpression of Rab7a increases vimentin phosphorylation and shifts vimentin to the soluble fraction; Rab7a silencing increases vimentin in the insoluble (assembled) fraction. CMT2B Rab7a mutants also increase soluble vimentin. Yeast two-hybrid; co-immunoprecipitation; GST pull-down with bacterially expressed recombinant proteins; immunofluorescence; subcellular fractionation Biochimica et biophysica acta High 23458836
2013 CMT2B Rab7 mutants dysregulate axonal transport (increased anterograde velocity and fraction), cause premature degradation of TrkA (reducing surface TrkA and NGF binding), and markedly impair NGF-induced Erk1/2 activation and PC12 cell differentiation, leading to axonal degeneration in DRG neurons. Live axonal transport imaging in E15.5 rat DRG neurons; surface TrkA measurement by flow cytometry; NGF binding assay; Erk1/2 phosphorylation; siRNA knockdown of Rab7 The Journal of neuroscience Medium 23616551
2013 Drosophila CMT2B rab7 mutants retain 10-50% function and cause neurodegeneration through partial loss of function (not gain of function). Loss of rab7 causes adult-onset neurodegeneration; overexpression of CMT2B mutants does not impair endosomal maturation but reduces efficient endosome recruitment. In vivo quantitative imaging, electrophysiology, and rescue experiments in Drosophila sensory and motor neurons; live endosome imaging eLife Medium 24327558
2012 RAB7A directly interacts with peripherin, a neuronal intermediate filament protein expressed primarily in peripheral neurons, via direct binding confirmed with recombinant proteins. CMT2B RAB7A mutants bind peripherin more strongly and alter its solubility, suggesting this interaction contributes to the neuronal specificity of CMT2B. Yeast two-hybrid screen with DRG cDNA library; co-immunoprecipitation; pull-down with recombinant proteins; siRNA knockdown and overexpression of RAB7A with peripherin solubility assay Acta neuropathologica High 23179371
2014 LKB1 kinase functions as a RAB7 effector, specifically binding GTP-bound RAB7 (not GDP-bound dominant-negative RAB7), and promotes formation of a complex between NRP-1 and RAB7 in late endosomes to facilitate lysosomal degradation of neuropilin-1 (NRP-1) and suppress angiogenesis. Co-immunoprecipitation of LKB1 with GTP- vs. GDP-bound RAB7 forms; RAB7 siRNA depletion and NRP-1 trafficking rescue; LKB1-NRP-1-RAB7 complex formation assays The Journal of clinical investigation Medium 25180605
2016 DEF8 interacts with PLEKHM1 and promotes its binding to RAB7; FAM98A and NDEL1 interact with PLEKHM1 to connect lysosomes to microtubules. Loss of PLEKHM1, DEF8, FAM98A, or NDEL1 impairs peripheral lysosome distribution and bone resorption in osteoclasts via RAB7. Co-immunoprecipitation; germline and conditional Plekhm1 knockout mice; lysosome positioning assays in osteoclasts; bone resorption assay JCI insight Medium 27777970
2016 Rab7a depletion causes slower cell migration, impaired cell adhesion and spreading on fibronectin, reduced β1-integrin activation and trafficking, decreased active Rac1 levels, and loss of properly oriented vimentin filaments during migration. siRNA knockdown of Rab7a in NCI H1299 cells; migration velocity and directness quantification; fibronectin adhesion/spreading assay; Rac1 activity (pull-down assay); β1-integrin localization and activation Biochimica et biophysica acta. Molecular cell research Medium 27888097
2016 Vps34 PI3K produces PI(3)P required to recruit the Rab7 GAP Armus (TBC1D2) to late endosomes. In Vps34-/- MEFs, failure to recruit Armus leads to hyperactivated Rab7 (elevated Rab7-GTP), enlarged late endosomes, failed ILV formation, and decreased EGFR degradation. Rab7 silencing or Armus overexpression rescues vacuolization. Vps34 knockout MEFs; Rab7-GTP pull-down assay; protein-lipid overlay and liposome binding assays for Armus-PI(3)P interaction; Rab7 siRNA rescue Journal of cell science High 27793976
2017 Rab7 is tyrosine-phosphorylated by Src kinase at Y183. This phosphorylation depends on Rab7's guanine nucleotide binding activity and Src kinase activity, is induced by EGF stimulation, and impairs Rab7 interaction with RILP, thereby inhibiting EGFR degradation and sustaining Akt signaling. In vitro Src kinase assay with Rab7; site-directed mutagenesis (Y183F); co-immunoprecipitation; EGF stimulation; EGFR degradation assay; Akt signaling readout Cellular signalling High 28336235
2017 WDR91 is a Rab7 effector that couples Rab switching with PI(3)P down-regulation on endosomes. WDR91 is recruited to endosomes by active GTP-Rab7 and inhibits Rab7-associated PI3K activity. Loss of WDR91 increases endosomal PI(3)P, arrests endosome maturation, and impairs neurite development in mouse neurons. Co-immunoprecipitation and pull-down of WDR91 with GTP-Rab7; Wdr91 knockout mice (global and brain-specific); PI(3)P measurement; endosome maturation assays; neurite complexity quantification; rescue with WDR91 vs. Rab7-binding mutants The Journal of cell biology High 28860274
2017 CD44s (CD44 short isoform) is internalized into endosomes and interacts with Rab7A to inhibit Rab7A-mediated EGFR trafficking to lysosomes and subsequent EGFR degradation. Co-immunoprecipitation; EGFR trafficking and degradation assays; siRNA knockdown of Rab7A Proceedings of the National Academy of Sciences of the United States of America Medium 28716909
2017 Rab7A regulates tau secretion: deletion of Rab7A decreases tau secretion, dominant-negative Rab7A decreases it, and constitutively active Rab7A increases it. Partial colocalization of tau with Rab7-positive structures indicates late endosomal involvement in tau secretion. Rab7A knockout and dominant-negative/constitutively active mutant expression in neurons and HeLa cells; tau secretion measurement; immunofluorescence colocalization Journal of neurochemistry Medium 28222213
2017 Retromer recruitment to endosomes requires binding to Rab7a. Inhibition of the Rab7a GAP TBC1D5 enhances Rab7a activation and leads to gain of retromer function (improved endosome-to-Golgi retrieval). TBC1D5 inhibition/knockdown; Rab7a activation state assay; retromer cargo retrieval assays Journal of cell science Medium 29777037
2018 Mitochondria-lysosome membrane contact sites form dynamically in healthy cells and are regulated by RAB7 GTP hydrolysis: active GTP-bound lysosomal RAB7 promotes contact formation, while contact untethering requires recruitment of the RAB7 GAP TBC1D15 to mitochondria via FIS1 to drive RAB7 GTP hydrolysis. Lysosomal contacts mark sites of mitochondrial fission. Electron microscopy, structured illumination microscopy, high-resolution confocal live-cell imaging; TBC1D15 and FIS1 recruitment assays; Rab7 GTP/GDP state manipulation; mitochondrial fission readouts Nature High 29364868
2018 TBK1 directly phosphorylates RAB7A at Ser72 upon mitochondrial depolarization in a PARKIN-dependent manner. Phospho-RAB7A(S72) loses association with RAB GDI, gains association with FLCN-FNIP1 (a DENN domain-containing GEF heterodimer), and is required for ATG9A recruitment to damaged mitochondria and efficient mitophagy. Global quantitative phosphoproteomics; in vitro TBK1 kinase assay; interaction proteomics with phosphomimetic (S72E) and non-phosphorylatable (S72A) RAB7A mutants; mitophagy flux assay; ATG9A recruitment imaging Science advances High 30627666
2017 Retromer-associated RAB7-specific GAP TBC1D5 controls RAB7 activity and localization; in absence of TBC1D5 or retromer, RAB7 hyperactivates and expands over the entire lysosomal domain. RAB7 also localizes to ER, trans-Golgi network, and mitochondrial membranes in a retromer/TBC1D5-dependent manner. Hyperactivated RAB7 disrupts ATG9a sorting and autophagosome formation around damaged mitochondria during Parkin-mediated mitophagy. TBC1D5 and retromer subunit KO cells; RAB7 localization by fluorescence microscopy; RAB7 mobility by FRAP; ATG9a sorting assay; mitophagy induction with CCCP The EMBO journal High 29158324
2019 Endosomal PI4P (produced primarily by PI4K2A) is required for Rab7 cycling on late endosomes; acute conversion of endosomal PI4P to PI(4,5)P2 causes Rab7 dissociation from late endosomes and releases PLEKHM1. PI4K2A deletion impairs PI(4,5)P2 production, causing defective Rab7 inactivation and increased autophagic structures with impaired autophagosome-lysosome fusion. Rab7 effectors Vps35 and RILP are not affected by acute PI(4,5)P2 production. Acute PI4P-to-PI(4,5)P2 conversion system; PI4K2A deletion; live-cell imaging of Rab7 and PLEKHM1 dynamics; LC3 structure quantification; autophagosome-lysosome fusion assay The EMBO journal High 31368593
2019 TBK1/IKKε phosphorylate RAB7 at S72 (same site as TBK1 in mitophagy context). Phosphomimetic RAB7-S72E shows diffuse cytosolic localization and enhanced innate immune signaling (STING pathway), while kinase-resistant RAB7-S72A localizes to active puncta promoting lysosomal STING degradation. Thus, RAB7-S72 phosphorylation limits STING turnover, increasing IRF3 target production. SILAC phosphoproteomics for substrate identification; phosphomimetic and phosphodead RAB7 mutants in TNBC cells; RAB7 localization imaging; STING levels and IRF3 targets (CXCL10, CCL5, IFNβ) measurement Cancer research Medium 31662325
2019 Rab7 depletion potently induces spontaneous ciliogenesis and cilia elongation and blocks serum-induced ciliary resorption. Active Rab7 is required for cilia disassembly via cilia tip excision (ectocytosis), which depends on F-actin polymerization at the excision site; Rab7 depletion prevents this F-actin polymerization. Rab7 siRNA knockdown; cilia length and number quantification; serum-induced cilia resorption assay; live imaging of cilia ectocytosis; F-actin staining at cilia tips; constitutively active Rab7 expression The Journal of cell biology Medium 31619485
2019 PIPKIγi5 (type Igamma PI4,5P2-kinase isoform 5) directly interacts with Rab7a and is required for Rab7a recruitment to early endosomes. Loss of PIPKIγi5 blocks early-to-late endosome maturation, disrupts retromer-Rab7a connection (preventing CI-M6PR retrograde sorting and hydrolase delivery to lysosomes), and blocks recruitment of Rab7a GAP TBC1D5 to late endosomes. Co-immunoprecipitation; PIPKIγi5 knockdown; Rab7a localization assay; retromer recruitment; CI-M6PR trafficking; autophagic degradation assay FASEB journal Medium 31908013
2020 Active Rab7 directly interacts with the NPC1 cholesterol transporter to license lysosomal cholesterol export. C18orf8 is a core subunit of the trimeric Mon1-Ccz1-C18orf8 (MCC) GEF complex required for Rab7 activation; C18orf8 deficiency abolishes Rab7 activation, disrupts late endosome morphology, and causes free cholesterol accumulation in swollen lysosomes (NPC1-like phenotype). A constitutively active Rab7 restores cholesterol export in MCC-deficient cells. Genome-wide CRISPR screen; co-immunoprecipitation of active Rab7 with NPC1; C18orf8, Ccz1, Mon1 deficiency by CRISPR; constitutively active Rab7 rescue; cholesterol trafficking assays Nature communications High 33144569
2020 CLN3 regulates endosomal function by modulating Rab7A-effector interactions: CLN3 is required for efficient Rab7A interaction with retromer (for endosome-to-TGN trafficking of lysosomal sorting receptors) and with PLEKHM1 (required for autophagosome-lysosome fusion). Loss of CLN3 or disease-causing CLN3 mutations cause degradation of lysosomal sorting receptors. Co-immunoprecipitation in live cells; CLN3 knockout and disease-mutation expression; lysosomal sorting receptor trafficking assay; autophagosome-lysosome fusion assay Journal of cell science Medium 32034082
2021 C5orf51 (RMC1) is a specific interactor of GDP-locked RAB7A and a component of the MON1-CCZ1 GEF complex. In absence of C5orf51, RAB7A localization on depolarized mitochondria is compromised and RAB7A is degraded by the proteasome. C5orf51 depletion also inhibits ATG9A recruitment to depolarized mitochondria. Proximity-dependent biotinylation (miniTurbo) with GDP-locked RAB7A; C5orf51 co-immunoprecipitation with MON1/CCZ1; C5orf51 KO cells; RAB7A stability and localization assay on depolarized mitochondria; ATG9A recruitment assay Autophagy Medium 34432599
2021 Crystal structure of human PDZD8 coiled-coil domain in complex with GTP-bound Rab7 reveals that two Rab7 molecules bind the opposite sides of the PDZD8 CC in a 2:1 ratio, with the switch I/II and interswitch regions forming the binding interface, consistent with GTP-dependent ER-late endosome tethering. Crystal structure determination; isothermal titration calorimetry (ITC); mutational analysis of binding interface; GTP-dependence validation Scientific reports High 34552186
2021 RAB7 activity is required for mitophagy in oocyte meiosis. PRKN (Parkin) ubiquitinates and degrades RAB7A and its GEF complex (CCZ1-MON1) through the ubiquitin-proteasome system, thereby limiting mitophagy. Active RAB7A (Q67L) or RAB7 activator ML098 rescues mitophagy defects and meiotic blockade caused by CCCP. mRNA microinjection of RAB7Q67L in oocytes; ML098 treatment; CCCP-induced mitophagy; meiosis stage imaging; PINK1/PRKN/RAB7 protein level measurement; ubiquitin-proteasome inhibition rescue Autophagy Medium 34229552
2021 RAB7A regulates vimentin phosphorylation through AKT and PAK1 kinases. RAB7A overexpression increases vimentin phosphorylation at Ser38 via AKT activity. RAB7A modulation also affects downstream effectors: β-catenin, caspase 9 expression, cofilin-1, and MMP2 activity. RAB7A overexpression and siRNA knockdown; AKT and PAK1 activity assays; vimentin phospho-site specific antibodies; beta-catenin, caspase 9, cofilin-1 Western blots; MMP2 zymography Cancers Medium 34066419
2024 TBK1 phosphorylation of RAB7A at S72 functions as a phosphoswitch during Parkin-dependent mitophagy: phospho-RAB7A abrogates binding to Rubicon (a negative autophagy regulator with RH domain), while Pacer (a positive regulator with RH domain) specifically binds phospho-RAB7A. In vitro TBK1 phosphorylation of RAB7A blocks Rubicon:RAB7A binding; Pacer knockout reduces Parkin mitophagy but not bulk autophagy; rescue requires an intact phosphate-binding basic triad in Pacer. Crystal structure of Rubicon:RAB7A complex; in vitro TBK1 phosphorylation; co-immunoprecipitation of Rubicon/Pacer with pRAB7A; Pacer KO cells; Parkin mitophagy assay; rescue with Pacer phosphate-binding mutant The Journal of cell biology High 38728007
2024 SIRT5-catalyzed desuccinylation of RAB7A at lysine 31 is required for RAB7A activity. Cadmium exposure decreases SIRT5 expression, increasing RAB7A succinylation at K31 and inhibiting RAB7A activity, thereby blocking autophagosome-lysosome fusion and autophagic clearance. SIRT5 overexpression restores RAB7A activity and autophagic flux. Proteomic identification of succinylation site; SIRT5 overexpression and knockdown; RAB7A activity assays; autophagosome-lysosome fusion assay; in vivo FAD4T mouse model Advanced science (Weinheim, Baden-Wurttemberg, Germany) Medium 38837686
2024 SARS-CoV-2 ORF3a, in complex with Vps39, sequesters the Rab7 GAP TBC1D5, preventing GTP hydrolysis and causing Rab7 hyperactivation. Hyperactive Rab7 impairs CI-M6PR retrieval from late endosomes to TGN, disrupts lysosomal hydrolase delivery, reduces Rab7-Arl8b compartment tethering, and promotes viral egress. The Rab7 GDP-locked mutant strongly reduces viral replication. SARS-CoV-2 infection and ORF3a expression; co-immunoprecipitation of ORF3a-Vps39-TBC1D5; Rab7 activation state assay; CI-M6PR trafficking; lysosomal hydrolase delivery assay; Rab7 GDP mutant viral replication assay Nature communications High 38448435
2024 TBK1 is recruited to lysosomes in response to elevated amino acid levels, where it phosphorylates Rab7 at S72 to alleviate Rab7-mediated inhibition of amino acid-dependent mTORC1 activation. An ALS/FTD-associated TBK1 E696K mutant constitutively accumulates at lysosomes, causing elevated Rab7 S72 phosphorylation and increased mTORC1 activation. Lysosomal TBK1 fractionation; Rab7 S72 phosphorylation assay under amino acid conditions; mTORC1 activation readout; TBK1 E696K mutant analysis The EMBO journal Medium 39103493
2024 USP4 deubiquitinase maintains RAB7A in an active deubiquitinated state; reduced USP4 in periodontitis leads to RAB7A ubiquitination, impairing lysosomal trafficking and autophagosome-lysosome fusion. RAB7A inactivation directly reduces autophagy efficiency and drives periodontitis progression. Proteomics identification of USP4-RAB7A interaction; USP4 and RAB7A knockdown/overexpression; ubiquitination assay; autophagosome-lysosome fusion assay; experimental periodontitis mouse model Autophagy Medium 39663592
2011 Rab7A is required for efficient HIV-1 production. Rab7A depletion by siRNA impairs Env glycoprotein processing, reduces incorporation of mature Env into viral particles, decreases HIV-1 infectivity, and induces a BST2/Tetherin-like phenotype on HIV-1 release, suggesting Rab7A contributes to the mechanism by which Vpu counteracts BST2/Tetherin. RNA interference targeting Rab7A; virological assays (Env processing, viral particle infectivity); BST2/Tetherin release assay PLoS pathogens Medium 22072966
2015 Rab7a and Rab27b control microRNA (miR-143) secretion through extracellular vesicles in shear-stressed endothelial cells, in a KLF2-dependent manner. siRNA knockdown of Rab7a and Rab27b; miR-143 secretion measurement in extracellular vesicle fractions; KLF2 pathway context FEBS letters Low 26348397
2018 Rab7a is required for degradation of dendritic cargo proteins (Nsg1, Nsg2): Rab7 activity mobilizes distal pre-degradative late endosomes for retrograde transport to the soma for terminal lysosomal degradation. Most dendritic LAMP1 endosomes are not degradative lysosomes. Rab7 dominant-negative expression in neurons; live imaging of late endosomes; degradation assay for Nsg1/Nsg2; colocalization of LAMP1 with CatB/D The Journal of cell biology Medium 29907658
2022 Dynein motor activity is required for Rab7-dependent retrograde transport of late endosomes in dendrites and for endosome maturation. Inhibiting dynein (directly or via RILP C-terminus expression) delays somatic endosome maturation (evidenced by excess Rab7 accumulation) and inhibits degradation of dendritic cargos. Rab7 GDP-GTP cycling appears necessary for late endosome fusion with lysosomes subsequent to somatic arrival. Multiple dynein/dynactin inhibition approaches (drugs, RILP C-terminus dominant negative); quantitative live imaging of late endosomes in dendrites; cargo degradation assays in hippocampal neurons The Journal of neuroscience Medium 35474277
2017 Rab7 GTPase directly interacts with mTOR at its N-terminal heat repeat domain. Rab7 GTPase knockdown in LAL-deficient myeloid cells reverses altered lysosome/mTOR distribution, reduces mTOR hyperactivation, and decreases downstream S6 phosphorylation, glucose consumption, and ROS production. Co-immunoprecipitation; GST pull-down with recombinant GST-Rab7 fusion protein; siRNA knockdown; lysosome distribution and mTOR co-localization; metabolic assays Oncotarget Medium 28415797

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Rab7: a key to lysosome biogenesis. Molecular biology of the cell 896 10679007
2018 Mitochondria-lysosome contacts regulate mitochondrial fission via RAB7 GTP hydrolysis. Nature 695 29364868
2006 Rab5 and Rab7 control endocytic sorting along the axonal retrograde transport pathway. Neuron 392 17046692
2012 Maturation of autophagosomes and endosomes: a key role for Rab7. Biochimica et biophysica acta 343 23220125
2016 Multiple Roles of the Small GTPase Rab7. Cells 336 27548222
2003 Modulation of Rab5 and Rab7 recruitment to phagosomes by phosphatidylinositol 3-kinase. Molecular and cellular biology 272 12640132
2003 Differential requirements of Rab5 and Rab7 for endocytosis of influenza and other enveloped viruses. Traffic (Copenhagen, Denmark) 186 12713661
2010 Rab7: role of its protein interaction cascades in endo-lysosomal traffic. Cellular signalling 181 20851765
2018 RAB7A phosphorylation by TBK1 promotes mitophagy via the PINK-PARKIN pathway. Science advances 180 30627666
2017 Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy. The EMBO journal 163 29158324
2008 Functional characterization of Rab7 mutant proteins associated with Charcot-Marie-Tooth type 2B disease. The Journal of neuroscience : the official journal of the Society for Neuroscience 147 18272684
2003 Human VPS34 and p150 are Rab7 interacting partners. Traffic (Copenhagen, Denmark) 140 14617358
2010 Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain. Molecular biology of the cell 131 20943950
2009 Rab7: roles in membrane trafficking and disease. Bioscience reports 128 19392663
2018 Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes. The Journal of cell biology 110 29907658
2003 Rab7 prevents growth factor-independent survival by inhibiting cell-autonomous nutrient transporter expression. Developmental cell 109 14536059
2010 Regional selectivity of rab5 and rab7 protein upregulation in mild cognitive impairment and Alzheimer's disease. Journal of Alzheimer's disease : JAD 106 20847427
2021 RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging. Autophagy 89 34229552
2019 Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion. The EMBO journal 87 31368593
2013 Defective axonal transport of Rab7 GTPase results in dysregulated trophic signaling. The Journal of neuroscience : the official journal of the Society for Neuroscience 87 23616551
2018 Revisiting Rab7 Functions in Mammalian Autophagy: Rab7 Knockout Studies. Cells 83 30463228
2020 A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export. Nature communications 78 33144569
2003 Rabring7, a novel Rab7 target protein with a RING finger motif. Molecular biology of the cell 77 12972561
2016 PLEKHM1/DEF8/RAB7 complex regulates lysosome positioning and bone homeostasis. JCI insight 76 27777970
2006 Rab7 and Rab27a control two motor protein activities involved in melanosomal transport. Pigment cell research 74 16965270
2001 Rab7 regulates phagosome maturation in Dictyostelium. Journal of cell science 71 11559753
2015 Rab7a and Rab27b control secretion of endothelial microRNA through extracellular vesicles. FEBS letters 69 26348397
2016 Vps34 regulates Rab7 and late endocytic trafficking through recruitment of the GTPase-activating protein Armus. Journal of cell science 68 27793976
2013 Charcot-Marie-Tooth 2B mutations in rab7 cause dosage-dependent neurodegeneration due to partial loss of function. eLife 67 24327558
2018 Inhibition of TBC1D5 activates Rab7a and can enhance the function of the retromer cargo-selective complex. Journal of cell science 64 29777037
2016 Rab7 induces clearance of α-synuclein aggregates. Journal of neurochemistry 64 27333324
2024 SIRT1-Rab7 axis attenuates NLRP3 and STING activation through late endosomal-dependent mitophagy during sepsis-induced acute lung injury. International journal of surgery (London, England) 63 38445453
2018 This Is the End: Regulation of Rab7 Nucleotide Binding in Endolysosomal Trafficking and Autophagy. Frontiers in cell and developmental biology 58 30333976
2016 Rab7a regulates cell migration through Rac1 and vimentin. Biochimica et biophysica acta. Molecular cell research 58 27888097
2011 Rab7A is required for efficient production of infectious HIV-1. PLoS pathogens 56 22072966
2013 Vimentin phosphorylation and assembly are regulated by the small GTPase Rab7a. Biochimica et biophysica acta 54 23458836
2010 CMT2B-associated Rab7 mutants inhibit neurite outgrowth. Acta neuropathologica 54 20464402
2017 Rab7A regulates tau secretion. Journal of neurochemistry 52 28222213
2019 Phosphorylation of RAB7 by TBK1/IKKε Regulates Innate Immune Signaling in Triple-Negative Breast Cancer. Cancer research 51 31662325
2019 Role of the RAB7 Protein in Tumor Progression and Cisplatin Chemoresistance. Cancers 50 31374919
2009 Rab7 and the CMT2B disease. Biochemical Society transactions 50 19754445
2014 Protein kinase LKB1 promotes RAB7-mediated neuropilin-1 degradation to inhibit angiogenesis. The Journal of clinical investigation 47 25180605
2019 LRP6 regulates Rab7-mediated autophagy through the Wnt/β-catenin pathway to modulate trophoblast cell migration and invasion. Journal of cellular biochemistry 45 31544984
2012 Charcot-Marie-Tooth type 2B disease-causing RAB7A mutant proteins show altered interaction with the neuronal intermediate filament peripherin. Acta neuropathologica 45 23179371
2024 SIRT5-Mediated Desuccinylation of RAB7A Protects Against Cadmium-Induced Alzheimer's Disease-Like Pathology by Restoring Autophagic Flux. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 44 38837686
2016 Charcot-Marie-Tooth 2b associated Rab7 mutations cause axon growth and guidance defects during vertebrate sensory neuron development. Neural development 44 26791407
2012 Rab7 mutants associated with Charcot-Marie-Tooth disease cause delayed growth factor receptor transport and altered endosomal and nuclear signaling. The Journal of biological chemistry 43 23188822
2017 Internalized CD44s splice isoform attenuates EGFR degradation by targeting Rab7A. Proceedings of the National Academy of Sciences of the United States of America 42 28716909
2010 Rab7 mutants associated with Charcot-Marie-Tooth disease exhibit enhanced NGF-stimulated signaling. PloS one 42 21151572
2017 WDR91 is a Rab7 effector required for neuronal development. The Journal of cell biology 41 28860274
2016 Iron overload causes endolysosomal deficits modulated by NAADP-regulated 2-pore channels and RAB7A. Autophagy 40 27383256
2000 Phenotype-genotype correlations in a CMT2B family with refined 3q13-q22 locus. Neurology 39 11094113
2024 SARS-CoV-2 virulence factor ORF3a blocks lysosome function by modulating TBC1D5-dependent Rab7 GTPase cycle. Nature communications 38 38448435
2011 Proteomics approach to identify the interacting partners of cellular prion protein and characterization of Rab7a interaction in neuronal cells. Journal of proteome research 38 21604690
2019 Rab7 regulates primary cilia disassembly through cilia excision. The Journal of cell biology 36 31619485
2015 CREG1 ameliorates myocardial fibrosis associated with autophagy activation and Rab7 expression. Biochimica et biophysica acta 36 25774384
2021 C5orf51 is a component of the MON1-CCZ1 complex and controls RAB7A localization and stability during mitophagy. Autophagy 35 34432599
2020 CLN3 regulates endosomal function by modulating Rab7A-effector interactions. Journal of cell science 35 32034082
2002 Tyrosinase and tyrosinase-related protein 1 require Rab7 for their intracellular transport. The Journal of investigative dermatology 35 12190873
2021 ATF3 induces RAB7 to govern autodegradation in paligenosis, a conserved cell plasticity program. EMBO reports 34 34309175
2019 Rab7a and Mitophagosome Formation. Cells 34 30857122
2023 Timosaponin AIII induces lipid peroxidation and ferroptosis by enhancing Rab7-mediated lipophagy in colorectal cancer cells. Phytomedicine : international journal of phytotherapy and phytopharmacology 33 37863004
2020 Rab7 controls lipid droplet-phagosome association during mycobacterial infection. Biochimica et biophysica acta. Molecular and cell biology of lipids 32 32229179
2021 The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7. Cell reports 31 34731620
2014 Human Rab7 mutation mimics features of Charcot-Marie-Tooth neuropathy type 2B in Drosophila. Neurobiology of disease 31 24521780
2024 Targeting Rab7-Rilp Mediated Microlipophagy Alleviates Lipid Toxicity in Diabetic Cardiomyopathy. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 29 38837607
2018 Rab7a modulates ER stress and ER morphology. Biochimica et biophysica acta. Molecular cell research 29 29505800
2017 Rab7 GTPase controls lipid metabolic signaling in myeloid-derived suppressor cells. Oncotarget 29 28415797
2024 RAB7 deficiency impairs pulmonary artery endothelial function and promotes pulmonary hypertension. The Journal of clinical investigation 28 38015641
2017 Tyrosine phosphorylation of Rab7 by Src kinase. Cellular signalling 28 28336235
2009 Thiazolidinediones induce Rab7-RILP-MAPK-dependent juxtanuclear lysosome aggregation and reduce tumor cell invasion. Traffic (Copenhagen, Denmark) 28 20015112
2009 Phylogeny and evolution of Rab7 and Rab9 proteins. BMC evolutionary biology 27 19442299
2020 Charcot-Marie-Tooth Type 2B: A New Phenotype Associated with a Novel RAB7A Mutation and Inhibited EGFR Degradation. Cells 26 32326241
2014 A novel RAB7 mutation in a Chinese family with Charcot-Marie-Tooth type 2B disease. Gene 26 24498653
2024 A RAB7A phosphoswitch coordinates Rubicon Homology protein regulation of Parkin-dependent mitophagy. The Journal of cell biology 24 38728007
2022 Dynein Is Required for Rab7-Dependent Endosome Maturation, Retrograde Dendritic Transport, and Degradation. The Journal of neuroscience : the official journal of the Society for Neuroscience 24 35474277
2022 Polarized α-synuclein trafficking and transcytosis across brain endothelial cells via Rab7-decorated carriers. Fluids and barriers of the CNS 24 35637478
2023 Rab7a-mTORC1 signaling-mediated cholesterol trafficking from the lysosome to mitochondria ameliorates hepatic lipotoxicity induced by aflatoxin B1 exposure. Chemosphere 23 36754296
2023 Rab7/Retromer-based endolysosomal trafficking is essential for proper host invasion in rice blast. The New phytologist 23 37291895
2023 Regulation of Endosomal Trafficking by Rab7 and Its Effectors in Neurons: Clues from Charcot-Marie-Tooth 2B Disease. Biomolecules 23 37759799
2021 Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1. Signal transduction and targeted therapy 23 33859171
2019 Knockdown of Rab7a suppresses the proliferation, migration, and xenograft tumor growth of breast cancer cells. Bioscience reports 22 29769411
2023 Lipophagy-related gene RAB7A is involved in immune regulation and malignant progression in hepatocellular carcinoma. Computers in biology and medicine 21 37044053
2021 RAB7A Regulates Vimentin Phosphorylation through AKT and PAK. Cancers 21 34066419
2005 SPTLC1 and RAB7 mutation analysis in dominantly inherited and idiopathic sensory neuropathies. Journal of neurology, neurosurgery, and psychiatry 21 15965219
2016 Strain-Specific Altered Regulatory Response of Rab7a and Tau in Creutzfeldt-Jakob Disease and Alzheimer's Disease. Molecular neurobiology 20 26768426
2012 Autonomic nervous system involvement in a new CMT2B family. Journal of the peripheral nervous system : JPNS 20 22971099
2022 Gamma-glutamyltransferase 7 suppresses gastric cancer by cooperating with RAB7 to induce mitophagy. Oncogene 18 35662282
2021 Rab7 reduces α-synuclein toxicity in rats and primary neurons. Experimental neurology 18 34695425
2022 Autophagy and Lysosomal Functionality in CMT2B Fibroblasts Carrying the RAB7K126R Mutation. Cells 17 35159308
2022 Protein phosphatase 2A-B56γ-Drp1-Rab7 signaling axis regulates mitochondria-lysosome crosstalk to sensitize the anti-cancer therapy of hepatocellular carcinoma. Biochemical pharmacology 17 35697120
2021 Structural basis of human PDZD8-Rab7 interaction for the ER-late endosome tethering. Scientific reports 17 34552186
2019 Control of Rab7a activity and localization through endosomal type Igamma PIP 5-kinase is required for endosome maturation and lysosome function. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 17 31908013
2023 SIRT1 mediated gastric cancer progression under glucose deprivation through the FoxO1-Rab7-autophagy axis. Frontiers in oncology 16 37293593
2021 Vps34 Inhibits Hepatocellular Carcinoma Invasion by Regulating Endosome-Lysosome Trafficking via Rab7-RILP and Rab11. Cancer research and treatment 16 33781048
2020 IFNB/interferon-β regulates autophagy via a MIR1-TBC1D15-RAB7 pathway. Autophagy 16 31958036
2000 Trypanosoma cruzi: cloning and characterization of a RAB7 gene. Experimental parasitology 16 11038317
2024 Lysosomal TBK1 responds to amino acid availability to relieve Rab7-dependent mTORC1 inhibition. The EMBO journal 15 39103493
2024 USP4 depletion-driven RAB7A ubiquitylation impairs autophagosome-lysosome fusion and aggravates periodontitis. Autophagy 15 39663592
2022 HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury. Autophagy 15 36048753

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