Affinage

LAMP1

Lysosome-associated membrane glycoprotein 1 · UniProt P11279

Audit flag: model fault
Length
417 aa
Mass
44.9 kDa
Annotated
2026-06-13
100 papers in source corpus 31 papers cited in narrative 31 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

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Mechanism profile

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Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 The 11-amino acid cytoplasmic tail of LAMP1 contains a tyrosine-based YXXI sorting motif that is sufficient for lysosomal targeting. Mutants retaining only the RKR membrane anchor and YXXI motif still localize to dense lysosomes. However, deleting one amino acid or adding five amino acids to alter the spacing of the YXXI motif relative to the membrane almost completely abolishes lysosomal targeting, trapping LAMP1 in a recycling pathway between the plasma membrane and early endocytic compartments. Site-directed mutagenesis of cytoplasmic tail, pulse-chase kinetics, subcellular fractionation, immunofluorescence The Journal of cell biology High 8647888
1996 The tyrosine-based lysosomal targeting signal in the LAMP1 cytoplasmic tail mediates sorting into AP-1-positive clathrin-coated vesicles at the trans-Golgi network. The cytosolic domain of LAMP1 binds both AP-1 and AP-2 adaptors, and LAMP1 is present in AP-1-positive vesicles/tubules in the trans-Golgi region. AP-1 binding and localization to AP-1 CCVs require the functional tyrosine-based signal. Co-immunoprecipitation (cytosolic domain binding to AP-1/AP-2), immunogold electron microscopy, adaptor binding assays The EMBO journal High 8895568
1992 The majority of newly synthesized LAMP1 is directly transported from the trans-Golgi network to lysosomes (half-time ~60 min), bypassing the plasma membrane. A minor fraction (~minority) is first transported to the cell surface and then internalized to reach lysosomes via the endocytic pathway (half-time >2 h). After granulocytic differentiation, direct intracellular sorting becomes more efficient, leaving only a minute fraction at the cell surface. Pulse-chase metabolic labeling, cell surface biotinylation, Percoll density gradient fractionation Archives of biochemistry and biophysics High 1632650
1999 Asparagine-linked oligosaccharides protect LAMP1 and LAMP2 from intracellular proteolysis within lysosomes. Removal of Asn-linked glycans from fully folded LAMP1 and LAMP2 in living cells using endoglycosidase H results in their rapid degradation, whereas the related LIMP-2 remains relatively stable. Depletion of both LAMPs had no measurable effect on endosomal/lysosomal pH, osmotic stability, or density, but delayed transport of endocytosed material to dense lysosomes. Endoglycosidase H treatment in live cells, pulse-chase degradation assays, pH and osmotic stability measurements The Journal of biological chemistry High 10521503
1998 Newly synthesized LAMP1 and LAMP2 are sorted at the trans-Golgi network into transport vesicles that are distinct from mannose-6-phosphate receptor/gamma-adaptin (AP-1 clathrin-coated) vesicles. LAMP vesicle generation required ATP, cytosol, was temperature-dependent and brefeldin A-sensitive. Wortmannin inhibited MPR/gamma-adaptin vesicle production but had no effect on LAMP vesicle generation, demonstrating separate TGN sorting pathways for LAMPs versus MPRs despite both using tyrosine-based motifs. In vitro TGN vesicle generation assay with tritiated CMP-sialic acid labeling, Nycodenz gradient sedimentation, wortmannin inhibition The Journal of biological chemistry High 9668075
2004 Newly synthesized LAMP1 traffics from the trans-Golgi network directly to early endosomes prior to delivery to late endosomes and lysosomes. Using a LAMP1 chimera (YAL) with tyrosine sulfation motifs fused to avidin, labeled chimera was captured by biotinylated probes endocytosed for only 5 min (early endosomes). In vitro fusion assays showed TGN-derived vesicles can fuse with early endosomes but not late endosomes or lysosomes. Novel LAMP1 chimera trafficking assay, in vitro TGN-to-endosome fusion reconstitution, biotinylated endocytic probe capture Traffic (Copenhagen, Denmark) High 15296493
2013 LAMP1/CD107a is required for efficient perforin delivery to lytic granules in NK cells. LAMP1 RNAi causes inhibition of NK-cell cytotoxicity, failure to deliver granzyme B to target cells, decreased perforin (but not granzyme B) levels in granules, and retention of perforin in trans-Golgi network-derived transport vesicles. Disruption of LAMP1's binding partner AP-1 sorting complex also causes perforin retention in transport vesicles, indicating that AP-1/LAMP1 interaction on transport vesicle surfaces is required for perforin trafficking to lytic granules. RNA interference (LAMP1 RNAi), immunofluorescence, cytotoxicity assays, granzyme B delivery assay, AP-1 disruption Blood High 23632890
2013 Surface CD107a/LAMP1 protects NK cells from degranulation-associated self-destruction. Engineered surface expression of CD107a/LAMP1 (but not CD107b/LAMP2) reduces granule-mediated killing of transfected target cells and reduces perforin binding to cells; this protection depends on glycosylation of the CD107a/LAMP1 hinge. Knockdown of CD107a/LAMP1 in primary human NK cells and deficiency in mice results in increased NK cell apoptosis upon target cell-induced degranulation. Engineered surface expression constructs, glycosylation-deficient mutants, LAMP1 knockdown in primary NK cells, LAMP1-deficient mouse NK cells, perforin binding assay, apoptosis measurement Blood High 23847195
2005 BLOC-3 (the HPS1/HPS4 complex) is required for optimal microtubule-dependent movement of LAMP1-containing late endocytic organelles. BLOC-3-deficient fibroblasts show reduced perinuclear clustering of LAMP1-positive organelles and a lower frequency of microtubule-dependent movement events (toward and away from the perinuclear region), without affecting duration or speed of individual movement events. LAMP1-GFP overexpression causes aberrant aggregation of late endocytic organelles dependent on LAMP1 dimerization via its cytoplasmic tail-GFP. Quantitative image analysis of organelle distribution, time-lapse fluorescence microscopy of LAMP1-GFP in live cells, comparison of WT vs. BLOC-3-deficient fibroblasts, dimerization mutant (LAMP1-mGFP) Journal of cell science Medium 16249233
2007 LAMP1 and LAMP2 together are required for phagosome maturation and bacterial killing. In LAMP1/LAMP2 double-deficient fibroblasts, phagosomes containing Neisseria gonorrhoeae fail to acquire Rab7 and RILP, do not undergo dynein/dynactin-mediated centripetal movement, and remain peripheral, preventing bacterial killing. Single LAMP1- or LAMP2-deficient cells form phagosomes that gradually acquire microbicidal activity, indicating redundant functions. LAMP1/LAMP2 knockout mouse fibroblasts, siRNA knockdown, bacterial survival assay, Rab7/RILP recruitment immunofluorescence, microtubule transport analysis Cellular microbiology High 17506821
2000 Increased cell-surface expression of LAMP1 (via mutation of the lysosomal targeting motif) renders CHO cells more susceptible to Trypanosoma cruzi invasion in a microtubule-dependent fashion, and enhances Ca2+-triggered lysosome exocytosis. Mutation of critical residues in the LAMP1 cytoplasmic tail lysosome-targeting motif abolishes both the invasion enhancement and the enhanced lysosome exocytosis, indicating that LAMP1 cytoplasmic tail interactions (not the luminal domain) modulate T. cruzi entry by promoting lysosome-plasma membrane fusion. Transfection of WT and cytoplasmic tail mutant LAMP1 into CHO cells, T. cruzi invasion assay, beta-hexosaminidase exocytosis assay, microtubule inhibitor treatment Cellular microbiology High 11207602
2012 LAMP1 and LAMP2B are the most abundant interaction partners of the lysosomal polypeptide transporter TAPL (ABCB9), identified by proteomics. The interaction interface maps to the four-transmembrane N-terminal domain (TMD0) of TAPL; LAMP proteins bind TAPL independently. This interaction does not affect TAPL subcellular localization or peptide transport activity, but in LAMP-deficient cells TAPL half-life is reduced 5-fold due to increased lysosomal degradation, indicating LAMP proteins retain TAPL on the limiting membrane and prevent its sorting to intraluminal vesicles. Proteomic interactome, co-immunoprecipitation, domain mapping, LAMP-deficient cells, half-life measurements Journal of cell science High 22641697
2016 LAMP1 and LAMP2 subdomains adopt a unique β-prism fold (confirmed by structural analysis, consistent with DC-LAMP/LAMP3). The N-domain of LAMP1 is necessary for multimeric assembly of LAMPs, whereas the N-domain of LAMP2 is repressive for such assembly, revealing distinct assembly modes for LAMP1 versus LAMP2 that may underlie their different functions. Structural analysis (β-prism fold determination), immunoprecipitation-based N-domain truncation analysis of LAMP multimerization Biochemical and biophysical research communications Medium 27663661
1995 LAMP1 biosynthetic transport in rat hepatocytes proceeds via multiple convergence points with the endocytic pathway: a major direct intracellular route to late endosomes (t1/2 = 45 min) then lysosomes (t1/2 = 85 min); a minor peripheral route via early endosomes (t1/2 = 33 min) and cell surface (t1/2 = 32 min); and a retrograde delivery from late endosomes back to early endosomes before final lysosomal delivery. Pulse-chase metabolic labeling with kinetic analysis, subcellular fractionation into endosomal compartments Experimental cell research Medium 7556456
1990 LAMP1 is present on the surface of activated but not resting human platelets, co-localizing with the lysosomal enzyme beta-galactosidase (but not with alpha- or dense granule markers) by sucrose density gradient fractionation. Half-maximal surface expression is induced by thrombin concentrations that trigger lysosomal enzyme release, indicating LAMP1 surface exposure specifically marks lysosomal secretion upon platelet activation. Sucrose density gradient granule fractionation, co-localization with lysosomal enzyme beta-galactosidase, flow cytometry surface expression after agonist stimulation The Journal of biological chemistry Medium 2211717
2001 Neisseria pili induce a transient cytosolic Ca2+ flux in human epithelial cells that triggers lysosome exocytosis, rapidly redistributing LAMP1 from intracellular lysosomes to the cell surface, where it is cleaved by Neisseria IgA1 protease. Surface LAMP1 accessibility is thus controlled by Ca2+-regulated lysosomal exocytosis. Ca2+ flux measurement, lysosome exocytosis assay, LAMP1 surface redistribution immunofluorescence, IgA1 protease cleavage assay Cellular microbiology Medium 11298650
2002 Neisseria porin P1.B induces a Ca2+ flux in epithelial cells that stimulates exocytosis of early and late endosomes (not lysosomes), increasing LAMP1 on the cell surface by a mechanism distinct from pilus-induced lysosome exocytosis. This represents a separate Ca2+-dependent exocytic route that delivers LAMP1 to the plasma membrane for IgA1 protease cleavage. Ca2+ flux measurement, differential exocytosis assays (early/late endosome vs lysosome markers), LAMP1 surface measurement by flow cytometry Infection and immunity Medium 12379671
2004 Soluble LAMP1 carrying the cytoplasmic tail [LAMP1(+Tail)] in circulation aggregates and interacts with plasma proteins. Transthyretin, isolated by affinity chromatography with either recombinant LAMP1(-Tail) or a synthesized 14-amino acid LAMP1 cytoplasmic tail peptide, interacts specifically with the LAMP1 cytoplasmic tail. Only the tail-containing form aggregates, suggesting transthyretin (a homotetramer) may crosslink soluble LAMP1. Affinity chromatography, immunoassay quantification of LAMP1 forms, recombinant protein production The Biochemical journal Medium 15200388
2016 LAMP1 serves as a critical endosomal receptor for Lassa virus (LASV) at acidic pH. A crystal structure of LASV GP1 identified a unique histidine triad forming the LAMP1 binding site; mutation of this triad impairs LAMP1 recognition and reduces infectivity. LAMP1 binding promotes membrane fusion, and His230 of LAMP1 is required to engage the spike complex. The histidines also sense acidic pH, preventing premature spike triggering. X-ray crystallography of LASV GP1, site-directed mutagenesis of histidine triad, LAMP1 binding assays, infectivity assays Journal of virology High 25972533
2018 LAMP1 increases the efficiency of Lassa virus (LASV) infection by elevating the pH threshold for GPC-mediated fusion, enabling LASV to fuse in less acidic endosomal compartments. In wild-type (LAMP1+) cells, LASV entry occurs through less acidic endosomes than in LAMP1 KO cells. LAMP1 is not absolutely required for LASV fusion but substantially increases its efficiency by allowing viral exit from endosomes before encountering more acidic/proteolytic environments. LAMP1 knockout cells, cell-cell and pseudovirus-cell surface fusion assays, pH threshold measurement, endosomal pH monitoring mBio High 29295909
2022 Human LAMP1 accelerates the kinetics of Lassa virus small fusion pore formation and potently promotes fusion pore dilation. The soluble LAMP1 ectodomain accelerates nascent pore formation but fails to promote efficient pore dilation, whereas ectopic full-length hLAMP1 dramatically promotes both initial and full dilation of fusion pores in forced plasma membrane fusion assays, implicating the LAMP1 transmembrane domain in this late stage of LASV fusion. Single virus imaging, population-based fusion assays, pseudovirus and VLP systems, ectopic hLAMP1 expression, forced plasma membrane fusion at low pH, soluble ectodomain vs. full-length comparison PLoS pathogens High 35969633
2016 A small molecule inhibitor of Lassa fever virus entry targets LAMP1 directly (identified by photo-reactive probe cross-linking). LAMP1 binding to LASV glycoprotein is cholesterol-dependent; the inhibitor blocks infection by competing with cholesterol in LAMP1. Mutational analysis of a docking model identified a putative inhibitor binding site within the cholesterol-binding pocket of the LAMP1 domain that engages GP. Photo-reactive probe cross-linking to identify LAMP1 as drug target, cholesterol dependence biochemical assays, mutational analysis of LAMP1 cholesterol-binding pocket, docking model PLoS pathogens Medium 30265711
2011 Salmonella acquires LAMP1 on phagosomes through a mechanism involving the bacterial effector SipC binding specifically to host Syntaxin6 via its C terminus, recruiting Syntaxin6 and accessory molecules (VAMP2, Rab6, Rab8) to Salmonella-containing phagosomes (SCP) to enable fusion with LAMP1-containing Golgi-derived vesicles. sipC knockout or sipC(M398K) mutant SCPs fail to recruit Syntaxin6 or acquire LAMP1. shRNA depletion of Syntaxin6 in macrophages significantly inhibits LAMP1 recruitment on SCP. Co-immunoprecipitation (SipC-Syntaxin6 interaction), bacterial mutants (sipC KO and point mutant), shRNA knockdown of Syntaxin6, immunofluorescence of LAMP1/Syntaxin6 recruitment, mouse infection model The Journal of biological chemistry High 22190682
2019 UBL4A causes lysosomal dysfunction by directly interacting with LAMP1, impairing autophagic degradation in pancreatic cancer cells. Co-immunoprecipitation confirmed physical interaction between UBL4A and LAMP1. LAMP1 overexpression reversed the antitumor (autophagy-inhibiting) effects of UBL4A, placing LAMP1 downstream of UBL4A in regulating lysosomal function and autophagic flux. Co-immunoprecipitation (UBL4A-LAMP1 interaction), LAMP1 overexpression rescue, Western blotting for autophagic flux markers Journal of experimental & clinical cancer research Medium 31288830
2019 SIRT1-mediated deacetylation of a lysine residue on the cytoplasmic domain of LAMP1 drives lipophagy and senescence in prostate cancer cells. AGG treatment induces cytoplasmic SIRT1, which deacetylates LAMP1's cytoplasmic domain, resulting in lipophagy-mediated free fatty acid accumulation and ROS generation that promotes senescence. SIRT1 inhibitor (sirtinol) treatment, mechanistic pathway experiments linking SIRT1 to LAMP1 deacetylation, lipophagy assays, ROS measurement Journal of cellular physiology Low 31544977
2016 Fucosylation of LAMP1 and LAMP2 by FUT1 (but not FUT2) regulates lysosomal positioning and autophagic flux in breast cancer cells. FUT1 knockdown causes LAMP1/LAMP2 to shift from peripheral to perinuclear distribution; this perinuclear positioning is correlated with decreased mTORC1 activity, increased autophagosome-lysosome fusion, and enhanced autophagic flux. LAMP1 and LAMP2 are confirmed substrates for FUT1 by targeted nanoLC-MS3 and MALDI-TOF analysis. FUT1 knockdown, targeted nanoLC-MS3 glycan analysis, MALDI-TOF, subcellular localization by imaging, mTORC1 activity measurement, autophagic flux assays Cell death & disease Medium 27560716
1999 In Pompe disease (lysosomal glycogen storage disorder) fibroblasts, LAMP1 glycoprocessing is retarded (t1/2 = 25 min vs. 17 min in controls) and trafficking to lysosomes is markedly delayed (t1/2 = 200 min vs. 100 min). A proportion of newly synthesized LAMP1 (5-8%) is trafficked out of cells; the extracellular soluble form lacks the cytoplasmic tail, whereas a soluble lysosomal pool also lacks the tail, suggesting clipping from the membrane. LAMP1 turnover is slower in Pompe cells (t1/2 = 4.9 days vs. 1.6 days in controls). Pulse-chase metabolic labeling, subcellular fractionation, turnover assays, tail-domain characterization of extracellular LAMP1 forms Molecular genetics and metabolism Medium 10066386
1994 Galectin-1 binds to LAMP1 (lysosome-associated membrane glycoprotein-1) as one of its major endogenous ligands in colon carcinoma cells, demonstrated by affinity chromatography of radiolabeled cell extracts on immobilized galectin-1 followed by immunoprecipitation from lactose-eluted material. Affinity chromatography on immobilized galectin-1, immunoprecipitation from lactose eluate, [3H]glucosamine radiolabeling Cancer research Medium 7954433
2015 Cell surface LAMP1 on high-metastatic B16F10 melanoma cells facilitates lung metastasis primarily through its polyLacNAc (poly-N-acetyllactosamine) carbohydrates that bind galectin-3. LAMP1 is the major carrier of polyLacNAc on these cells. shRNA-mediated LAMP1 knockdown decreases surface LAMP1, reduces galectin-3 binding to cell surface, impairs spreading and motility on galectin-3, and significantly reduces experimental lung metastasis. Overexpression of a mutant LAMP1 (Y386A) with low polyLacNAc fails to augment galectin-3 binding or lung metastasis. shRNA inducible knockdown, lentiviral mutant overexpression, flow cytometry, experimental lung metastasis assay, galectin-3 binding assay, spreading/motility on ECM components Journal of cancer research and clinical oncology Medium 25614122
2015 Extracellular galectin-3 induces MMP9 expression via p38 MAPK pathway signaling through cell-surface LAMP1. LAMP1 is identified as the key mediator because shRNA knockdown of LAMP1 (which is the major carrier of polyLacNAc, the galectin-3 ligand) abolishes galectin-3-induced MMP9 upregulation via p38 MAPK. shRNA knockdown of LAMP1, signaling pathway inhibitors, RT-PCR/Western blot for MMP9 Molecular and cellular biochemistry Medium 25739356
2022 Drosophila Lamp1 is a bona fide ortholog of vertebrate LAMP1/LAMP2 but, unlike lamp1/lamp2 double-mutant mice, Lamp1-deficient flies are viable and do not show autophagy defects. However, Lamp1 deficiency increases the number of acidic organelles and causes defects in lipid metabolism, with elevated sterols and diacylglycerols, indicating a role for LAMP1 in lipid transport rather than autophagy in Drosophila. Drosophila Lamp1 mutant characterization, autophagy assays (LC3/Atg8a puncta, autophagic vacuoles), acidic organelle quantification (LysoTracker), lipid profiling Autophagy Medium 35266854

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2004 CD107a as a functional marker for the identification of natural killer cell activity. Journal of immunological methods 1178 15604012
2006 Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine 762 16973206
2008 Relationship between CD107a expression and cytotoxic activity. Cellular immunology 379 18835598
2021 Gut-licensed IFNγ+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes. Nature 271 33408417
1996 The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. The Journal of cell biology 234 8647888
1999 Asparagine-linked oligosaccharides protect Lamp-1 and Lamp-2 from intracellular proteolysis. The Journal of biological chemistry 165 10521503
2005 Distribution and dynamics of Lamp1-containing endocytic organelles in fibroblasts deficient in BLOC-3. Journal of cell science 163 16249233
1988 Isolation and sequencing of a cDNA clone encoding lysosomal membrane glycoprotein mouse LAMP-1. Sequence similarity to proteins bearing onco-differentiation antigens. The Journal of biological chemistry 162 3379044
1996 The tyrosine-based lysosomal targeting signal in lamp-1 mediates sorting into Golgi-derived clathrin-coated vesicles. The EMBO journal 157 8895568
2018 Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy 141 29940787
2006 Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease. Blood 137 16778144
2013 LAMP1/CD107a is required for efficient perforin delivery to lytic granules and NK-cell cytotoxicity. Blood 132 23632890
2013 Surface CD107a/LAMP-1 protects natural killer cells from degranulation-associated damage. Blood 123 23847195
2005 Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell research 123 15916720
2017 Perforin and CD107a testing is superior to NK cell function testing for screening patients for genetic HLH. Blood 114 28270454
2016 Acidic pH-Induced Conformations and LAMP1 Binding of the Lassa Virus Glycoprotein Spike. PLoS pathogens 114 26849049
1985 Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells. Archives of biochemistry and biophysics 106 3923938
1996 Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium. Cellular immunology 99 8660832
1998 Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells. International journal of cancer 98 9426697
2011 Endo-lysosomal vesicles positive for Rab7 and LAMP1 are terminal vesicles for the transport of dextran. PloS one 91 22039519
1992 The lysosomal membrane glycoprotein lamp-1 is transported to lysosomes by two alternative pathways. Archives of biochemistry and biophysics 85 1632650
1997 Diagnosis of lysosomal storage disorders: evaluation of lysosome-associated membrane protein LAMP-1 as a diagnostic marker. Clinical chemistry 83 9267309
2006 Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathology and applied neurobiology 82 16972884
1994 Concomitant increases in galectin-1 and its glycoconjugate ligands (carcinoembryonic antigen, lamp-1, and lamp-2) in cultured human colon carcinoma cells by sodium butyrate. Cancer research 80 7954433
2015 Molecular Mechanism for LAMP1 Recognition by Lassa Virus. Journal of virology 77 25972533
2017 Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry. Journal of visualized experiments : JoVE 76 28784946
2004 Lysosome associated membrane protein 1 (Lamp1) traffics directly from the TGN to early endosomes. Traffic (Copenhagen, Denmark) 76 15296493
1995 The lysosomal membrane glycoproteins Lamp-1 and Lamp-2 are present in mobilizable organelles, but are absent from the azurophil granules of human neutrophils. The Biochemical journal 73 7487911
1998 Sorting of lysosomal membrane glycoproteins lamp-1 and lamp-2 into vesicles distinct from mannose 6-phosphate receptor/gamma-adaptin vesicles at the trans-Golgi network. The Journal of biological chemistry 72 9668075
1990 Identification and characterization of LAMP-1 as an activation-dependent platelet surface glycoprotein. The Journal of biological chemistry 72 2211717
2019 CD107a Degranulation Assay to Evaluate Immune Cell Antitumor Activity. Methods in molecular biology (Clifton, N.J.) 70 30465198
2019 Tanshinone IIA Restores Dynamic Balance of Autophagosome/Autolysosome in Doxorubicin-Induced Cardiotoxicity via Targeting Beclin1/LAMP1. Cancers 68 31261758
2018 Lamp1 Increases the Efficiency of Lassa Virus Infection by Promoting Fusion in Less Acidic Endosomal Compartments. mBio 66 29295909
2014 miR-184 regulates ezrin, LAMP-1 expression, affects phagocytosis in human retinal pigment epithelium and is downregulated in age-related macular degeneration. The FEBS journal 63 25251993
2016 Role of LAMP1 Binding and pH Sensing by the Spike Complex of Lassa Virus. Journal of virology 62 27605678
1993 Lysosome-associated membrane protein-1 (LAMP-1) is the melanocyte vesicular membrane glycoprotein band II. The Journal of investigative dermatology 62 8429232
2016 Lysosome-associated membrane proteins-1 and -2 (LAMP-1 and LAMP-2) assemble via distinct modes. Biochemical and biophysical research communications 60 27663661
2007 Cellular uptake of amelogenin, and its localization to CD63, and Lamp1-positive vesicles. Cellular and molecular life sciences : CMLS 60 17187173
2004 LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry. Part A : the journal of the International Society for Analytical Cytology 60 15351990
2014 Epigenetic silencing of microRNA-373 to epithelial-mesenchymal transition in non-small cell lung cancer through IRAK2 and LAMP1 axes. Cancer letters 59 25063738
2007 Arrested maturation of Neisseria-containing phagosomes in the absence of the lysosome-associated membrane proteins, LAMP-1 and LAMP-2. Cellular microbiology 57 17506821
2011 Measurement of NK activity in whole blood by the CD69 up-regulation after co-incubation with K562, comparison with NK cytotoxicity assays and CD107a degranulation assay. Journal of immunological methods 56 21839083
2019 UBL4A inhibits autophagy-mediated proliferation and metastasis of pancreatic ductal adenocarcinoma via targeting LAMP1. Journal of experimental & clinical cancer research : CR 54 31288830
2017 Mapping of the Lassa virus LAMP1 binding site reveals unique determinants not shared by other old world arenaviruses. PLoS pathogens 54 28448640
2014 Effects of cryopreservation on effector cells for antibody dependent cell-mediated cytotoxicity (ADCC) and natural killer (NK) cell activity in (51)Cr-release and CD107a assays. Journal of immunological methods 52 24561308
2013 Dystrophin deficiency leads to disturbance of LAMP1-vesicle-associated protein secretion. Cellular and molecular life sciences : CMLS 51 23344255
1995 Biosynthetic transport of a major lysosomal membrane glycoprotein, lamp-1: convergence of biosynthetic and endocytic pathways occurs at three distinctive points. Experimental cell research 51 7556456
2016 Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells. Cell death & disease 50 27560716
2016 Direct regulation of LAMP1 by tumor-suppressive microRNA-320a in prostate cancer. International journal of oncology 48 27212625
2014 Tartrate-resistant acid phosphatase (TRAP) co-localizes with receptor activator of NF-KB ligand (RANKL) and osteoprotegerin (OPG) in lysosomal-associated membrane protein 1 (LAMP1)-positive vesicles in rat osteoblasts and osteocytes. Histochemistry and cell biology 46 25201349
2013 Expression of the lysosomal-associated membrane protein-1 (LAMP-1) in astrocytomas. International journal of clinical and experimental pathology 45 23826410
2015 Role of tumor cell surface lysosome-associated membrane protein-1 (LAMP1) and its associated carbohydrates in lung metastasis. Journal of cancer research and clinical oncology 43 25614122
2019 Circ-LAMP1 promotes T-cell lymphoblastic lymphoma progression via acting as a ceRNA for miR-615-5p to regulate DDR2 expression. Gene 42 30922709
2012 The lysosomal polypeptide transporter TAPL is stabilized by interaction with LAMP-1 and LAMP-2. Journal of cell science 42 22641697
2000 Surface-targeted lysosomal membrane glycoprotein-1 (Lamp-1) enhances lysosome exocytosis and cell invasion by Trypanosoma cruzi. Cellular microbiology 42 11207602
2014 Increase of IFN-γ and TNF-α production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor. Cancer cell international 41 25302050
2009 A Francisella novicida pdpA mutant exhibits limited intracellular replication and remains associated with the lysosomal marker LAMP-1. Microbiology (Reading, England) 40 19372155
2003 Aspirin inhibits surface glycoprotein IIb/IIIa, P-selectin, CD63, and CD107a receptor expression on human platelets. Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis 40 12695747
1986 Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochemical Society symposium 40 3101702
2019 Deacetylation of LAMP1 drives lipophagy-dependent generation of free fatty acids by Abrus agglutinin to promote senescence in prostate cancer. Journal of cellular physiology 39 31544977
2022 Lamp1 mediates lipid transport, but is dispensable for autophagy in Drosophila. Autophagy 38 35266854
1993 The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes. The Journal of biological chemistry 38 8517882
2001 The pilus-induced Ca2+ flux triggers lysosome exocytosis and increases the amount of Lamp1 accessible to Neisseria IgA1 protease. Cellular microbiology 37 11298650
2019 Human papillomavirus 16E6/E7 activates autophagy via Atg9B and LAMP1 in cervical cancer cells. Cancer medicine 35 31215164
2018 Lysosomal LAMP1 immunoreactivity exists in both diffuse and neuritic amyloid plaques in the human hippocampus. The European journal of neuroscience 34 29570886
2015 Extracellular galectin-3 induces MMP9 expression by activating p38 MAPK pathway via lysosome-associated membrane protein-1 (LAMP1). Molecular and cellular biochemistry 33 25739356
2011 Salmonella acquires lysosome-associated membrane protein 1 (LAMP1) on phagosomes from Golgi via SipC protein-mediated recruitment of host Syntaxin6. The Journal of biological chemistry 33 22190682
2007 Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1. The Biochemical journal 33 17708745
2014 Monomethyl fumarate augments NK cell lysis of tumor cells through degranulation and the upregulation of NKp46 and CD107a. Cellular & molecular immunology 32 25435072
2019 Subclinical Cytomegalovirus DNA Is Associated with CD4 T Cell Activation and Impaired CD8 T Cell CD107a Expression in People Living with HIV despite Early Antiretroviral Therapy. Journal of virology 30 31019052
2017 CD3+CD4negCD8neg (double negative) T lymphocytes and NKT cells as the main cytotoxic-related-CD107a+ cells in lesions of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis. Parasites & vectors 30 28468680
2014 Regulation of melanoma metastasis to lungs by cell surface Lysosome Associated Membrane Protein-1 (LAMP1) via galectin-3. Biochemical and biophysical research communications 30 24845565
2017 Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes. Methods in molecular biology (Clifton, N.J.) 29 28456985
2015 CD107a as a marker of activation in chicken cytotoxic T cells. Journal of immunological methods 29 25743852
2020 Activity of human serum antibodies in an influenza virus hemagglutinin stalk-based ADCC reporter assay correlates with activity in a CD107a degranulation assay. Vaccine 28 31959425
2020 Abnormal LAMP1 glycosylation may play a role in Niemann-Pick disease, type C pathology. PloS one 28 31999726
2010 Full length amelogenin binds to cell surface LAMP-1 on tooth root/periodontium associated cells. Archives of oral biology 28 20382373
2021 Combination Immune Checkpoint Blockade Enhances IL-2 and CD107a Production from HIV-Specific T Cells Ex Vivo in People Living with HIV on Antiretroviral Therapy. Journal of immunology (Baltimore, Md. : 1950) 26 34853078
2021 Circ-LAMP1 contributes to the growth and metastasis of cholangiocarcinoma via miR-556-5p and miR-567 mediated YY1 activation. Journal of cellular and molecular medicine 25 33675150
2016 Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions. The Journal of neuroscience : the official journal of the Society for Neuroscience 24 27466344
2014 Vaccine-induced CD107a+ CD4+ T cells are resistant to depletion following AIDS virus infection. Journal of virology 24 25275131
1999 Altered trafficking and turnover of LAMP-1 in Pompe disease-affected cells. Molecular genetics and metabolism 24 10066386
2016 Recombinant DNA vaccine of Hantavirus Gn and LAMP1 induced long-term immune protection in mice. Antiviral research 23 27923570
1988 Pre-lysosomal divergence of alpha 2-macroglobulin and transferrin: a kinetic study using a monoclonal antibody against a lysosomal membrane glycoprotein (LAMP-1). The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 2450119
2009 Retention of viability, cytotoxicity, and response to IL-2, IL-15, or IFN-alpha by human NK cells after CD107a degranulation. Journal of leukocyte biology 22 19237639
2006 Expression of lysosome-associated membrane protein 1 (Lamp-1) and galectins in human keratinocytes is regulated by differentiation. Archives of dermatological research 21 16710742
2005 Immunochemical analysis of CD107a (LAMP-1). Cellular immunology 20 16168398
2002 Neisseria gonorrhoeae porin P1.B induces endosome exocytosis and a redistribution of Lamp1 to the plasma membrane. Infection and immunity 19 12379671
1993 Uvomorulin, LAMP-1, and laminin are substrates for cell surface beta-1,4-galactosyltransferase on F9 embryonal carcinoma cells: comparisons between wild-type and mutant 5.51 att- cells. Experimental cell research 19 8359222
2021 Activated γδ T Cells With Higher CD107a Expression and Inflammatory Potential During Early Pregnancy in Patients With Recurrent Spontaneous Abortion. Frontiers in immunology 18 34484234
2003 Differential expression of lysosomal associated membrane protein (LAMP-1) during mammalian spermiogenesis. Molecular reproduction and development 18 12950108
2015 Responsiveness of platelets during storage studied with flow cytometry--formation of platelet subpopulations and LAMP-1 as new markers for the platelet storage lesion. Vox sanguinis 17 26389538
2008 The effects of LAMP1 and LAMP3 on M180 amelogenin uptake, localization and amelogenin mRNA induction by amelogenin protein. Journal of biochemistry 17 18676354
1996 The lysosomal-associated membrane protein LAMP-1 is a novel differentiation marker for HC11 mouse mammary epithelial cells. Differentiation; research in biological diversity 17 8983177
2022 Human LAMP1 accelerates Lassa virus fusion and potently promotes fusion pore dilation upon forcing viral fusion with non-endosomal membrane. PLoS pathogens 16 35969633
2018 Critical role for cholesterol in Lassa fever virus entry identified by a novel small molecule inhibitor targeting the viral receptor LAMP1. PLoS pathogens 16 30265711
2009 Nef-mediated MHC class I down-regulation unmasks clonal differences in virus suppression by SIV-specific CD8(+) T cells independent of IFN-gamma and CD107a responses. Virology 16 19555986
2004 Transthyretin interacts with the lysosome-associated membrane protein (LAMP-1) in circulation. The Biochemical journal 16 15200388
2022 HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury. Autophagy 15 36048753
2022 Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo. eLife 15 36459486

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