Affinage

DDOST

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit · UniProt P39656

Round 2 corrected
Length
456 aa
Mass
50.8 kDa
Annotated
2026-04-28
90 papers in source corpus 23 papers cited in narrative 23 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

DDOST encodes OST48, a non-catalytic 48 kDa subunit of the oligosaccharyltransferase (OST) complex that is essential for N-linked glycosylation of nascent and newly translocated polypeptides in the endoplasmic reticulum. OST48 is required for the assembly and stability of both the cotranslational STT3A-containing and posttranslational STT3B-containing OST isoforms; its depletion causes pronounced hypoglycosylation across substrates including EGFR, PD-L1, and MITA/STING, the latter requiring DDOST-dependent glycosylation for oligomerization and antiviral signaling (PMID:22467853, PMID:36449507, PMID:41413687). ER retention of OST48 is mediated by a C-terminal cytosolic dilysine retrieval motif and by luminal-domain interaction with ribophorin I, and cryo-EM structures show that it is embedded in both OST-A and OST-B complexes whose architectures enable cotranslational versus posttranslational glycosylation (PMID:11530934, PMID:31831667). Loss-of-function mutations in DDOST cause a congenital disorder of glycosylation (DDOST-CDG), confirmed by genetic identification and complementation rescue in patient fibroblasts (PMID:22305527).

Mechanistic history

Synthesis pass · year-by-year structured walk · 9 steps
  1. 1992 High

    Identification of OST48 as a conserved integral-membrane subunit of the mammalian OST complex, and demonstration via yeast WBP1 depletion that this subunit is essential for N-glycosylation activity, established the foundational role of DDOST in the glycosylation machinery.

    Evidence Protein purification from canine microsomes with cDNA cloning; conditional WBP1 depletion in S. cerevisiae with in vivo and in vitro glycosylation assays

    PMID:1429707 PMID:1600939

    Open questions at the time
    • Precise structural role of OST48 within the OST complex was unknown
    • Whether OST48 has catalytic or purely structural function was unresolved
    • No human disease connection established
  2. 1996 High

    The surprising discovery that OST48 is identical to the AGE-binding protein p60 and is present on the cell surface expanded its functional repertoire beyond ER-resident N-glycosylation to extracellular AGE recognition.

    Evidence N-terminal sequencing, AGE-ligand binding assays, immunoprecipitation, flow cytometry, and antibody inhibition assays on rat liver membranes and multiple cell types

    PMID:8855306

    Open questions at the time
    • Mechanism of OST48 trafficking to the plasma membrane despite ER retention signals was unexplained
    • Whether AGE binding has physiological signaling consequences in vivo was unresolved
  3. 2001 High

    Defining two independent ER retention mechanisms — a C-terminal dilysine retrieval motif and luminal-domain interaction with ribophorin I — explained how OST48 is maintained in the ER and how its surface appearance could be regulated.

    Evidence Site-directed mutagenesis of C-terminal lysines and co-expression with ribophorin I in COS-1 cells with immunofluorescence

    PMID:11530934

    Open questions at the time
    • Whether regulated escape from ER retention contributes to AGE-receptor function was untested
    • Structural basis of ribophorin I–OST48 luminal interaction was unknown
  4. 2009 High

    Isoform-specific knockdown studies revealed that OST48 is a shared subunit of two functionally distinct OST complexes — STT3A (cotranslational) and STT3B (posttranslational) — that cooperate sequentially to maximize glycosylation efficiency.

    Evidence Isoform-specific siRNA knockdown with biosynthetic pulse labeling and glycosylation site-specific analysis in human cells

    PMID:19167329

    Open questions at the time
    • How OST48 contributes differentially to each isoform's assembly was unclear
    • Structural architecture of each complex was unresolved
  5. 2012 High

    Three advances established OST48's global structural role and disease relevance: knockdown showed it is required for stability of both OST isoforms; pathogenic DDOST mutations were identified as causative for CDG with rescue by wild-type cDNA; and ERAD was shown to degrade excess unassembled OST subunits to maintain stoichiometry.

    Evidence Subunit-specific siRNA with complex integrity and glycosylation readouts; whole-exome sequencing and cDNA complementation in patient fibroblasts; SILAC-SRM mass spectrometry in yeast

    PMID:22305527 PMID:22467853 PMID:25995378

    Open questions at the time
    • Genotype-phenotype relationships for different DDOST mutations were uncharacterized
    • Structural basis for OST48's role in complex assembly was not visualized
  6. 2017 Medium

    In vivo overexpression studies showed that excess OST48 drives hepatic and renal ER stress through AGE accumulation, connecting the AGE-receptor function to organ-level pathology.

    Evidence DDOST knock-in transgenic mice with high-AGE diet and podocyte-specific transgenic mice; metabolic phenotyping, electron microscopy, and glomerular proteomics

    PMID:28947796 PMID:34277994

    Open questions at the time
    • Whether ER stress results from AGE-binding activity or glycosylation imbalance was not distinguished
    • Relevance to human diabetic nephropathy or liver disease not confirmed genetically
  7. 2019 High

    High-resolution cryo-EM structures of both human OST-A and OST-B complexes revealed the precise position of OST48 within each and showed that ribophorin I forms a ribosome-contacting four-helix bundle in OST-A that is disordered in OST-B, providing a structural basis for cotranslational versus posttranslational glycosylation.

    Evidence Cryo-electron microscopy of purified human OST-A and OST-B complexes

    PMID:31831667

    Open questions at the time
    • Conformational dynamics of OST48 during catalytic cycle were not captured
    • No structure of OST48 in its AGE-binding conformation at the plasma membrane
  8. 2022 High

    Identification of MITA/STING as a specific DDOST-dependent glycosylation substrate demonstrated that OST48 directs substrate-selective glycosylation with functional consequences — MITA glycosylation was required for its oligomerization and antiviral signaling, and Ddost overexpression in vivo enhanced innate immunity against HSV-1.

    Evidence Site-directed mutagenesis of MITA N-glycosylation sites, DDOST knockdown/overexpression, HSV-1 infection assays, mouse HSV encephalitis model

    PMID:36449507

    Open questions at the time
    • How DDOST confers substrate selectivity for MITA versus other glycoproteins is mechanistically unclear
    • Whether this reflects STT3A- or STT3B-isoform-specific activity was not resolved
  9. 2025 Medium

    Extension to cancer biology showed that DDOST-dependent glycosylation of EGFR and PD-L1 sustains oncogenic signaling and immune evasion in hepatocellular carcinoma, while phosphorylation-dependent regulation of DDOST by PNKP was discovered in an atherosclerosis model, adding a post-translational regulatory layer.

    Evidence DDOST knockdown with EGFR/PD-L1 glycosylation and signaling analysis plus OST inhibitor treatment in xenograft models; Co-IP of PNKP-DDOST interaction in neutrophils with functional NET formation readout in ApoE-/- mice

    PMID:41109654 PMID:41413687

    Open questions at the time
    • Which DDOST phosphorylation sites are functionally relevant is not mapped
    • Whether PNKP-DDOST regulation operates in contexts beyond neutrophil NET formation is unknown
    • Clinical relevance of OST inhibition in cancer requires validation

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: the structural basis for OST48's reported AGE-binding activity at the plasma membrane and its relationship to its ER glycosylation function; the mechanism by which OST48 confers substrate selectivity for specific glycoproteins; and whether phosphorylation of DDOST constitutes a general regulatory mechanism for OST activity.
  • No structure of OST48 in its AGE-receptor conformation
  • Substrate selectivity mechanism for DDOST-dependent glycosylation is undefined
  • Phosphorylation-dependent regulation of DDOST activity lacks biochemical reconstitution

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 5 GO:0005198 structural molecule activity 2
Localization
GO:0005783 endoplasmic reticulum 3 GO:0005886 plasma membrane 1
Pathway
R-HSA-392499 Metabolism of proteins 6 R-HSA-1643685 Disease 3 R-HSA-168256 Immune System 1
Partners
DAD1LOXPNKPRPN1RPN2STT3ASTT3B
Complex memberships
OST-A (STT3A-containing OST complex)OST-B (STT3B-containing OST complex)

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1992 OST48 (DDOST) was identified as the 48-kDa subunit of the mammalian oligosaccharyltransferase complex, purified from canine microsomes alongside ribophorins I and II; it is an integral membrane protein with the majority of the polypeptide in the ER lumen and was found to be homologous to the essential yeast protein WBP1 (25% sequence identity), establishing it as a conserved OST component across eukaryotes. Protein purification, cDNA cloning, sequence analysis, membrane fractionation The Journal of biological chemistry High 1429707
1992 The yeast OST48 homolog WBP1 is an essential component of the oligosaccharyltransferase complex; depletion of WBP1 in S. cerevisiae reduced OST activity both in vivo (impaired glycosylation of carboxypeptidase Y and proteinase A) and in vitro (reduced transfer of core oligosaccharides to acceptor peptide in microsomal membranes). Conditional depletion of WBP1 in yeast, in vivo glycosylation assays, in vitro OST activity assay with microsomal membranes The EMBO journal High 1600939
1994 Genetic epistasis in yeast showed that combining kar2 (BiP) and wbp1 (OST48 ortholog) mutations produces a synergistic growth defect, and BiP was found to form a specific complex with non-glycosylated carboxypeptidase Y in kar2 mutants, demonstrating that BiP and N-linked glycosylation (mediated by the WBP1-containing OST complex) function in distinct but cooperating steps of secretory protein folding/processing. Yeast genetic epistasis (double mutants), co-immunoprecipitation of BiP with non-glycosylated substrate, in vivo secretory pathway assays European journal of biochemistry High 8020500
1995 The complete amino acid sequence of the human OST48 (DDOST) 50-kDa subunit was determined by chemical sequencing; it is 98% identical to the canine homolog, 93% to the avian homolog, and 25% to the yeast WBP1 beta subunit, confirming structural conservation of this OST subunit across all eukaryotes examined. Protein purification from human liver microsomes, chemical and enzymatic peptide sequencing Archives of biochemistry and biophysics High 7625827
1995 A Drosophila melanogaster homolog of OST48, designated DmOST50, was cloned by PCR; the deduced amino acid sequence shows 62.7% identity to canine Ost48p, 62.4% to avian AvOst50p, and 27.1% to yeast Wbp1p, demonstrating conservation of this OST subunit across metazoans and fungi. PCR cloning, cDNA library screening, sequence alignment Gene Medium 7607543
1996 OST48 (DDOST product) was identified as identical to the AGE-binding protein p60 isolated from rat liver membranes; immunoprecipitated OST48 from rough ER fractions exhibited both AGE-specific binding and immunoreactivity to anti-p60 antibody. Immune IgG raised to recombinant OST48 inhibited binding of AGE-BSA to cell membranes in a dose-dependent manner, and OST48 was detected on the surface of multiple cell types by immunostaining and flow cytometry. N-terminal protein sequencing, AGE-ligand binding assay, immunoprecipitation, Western blot, inhibition assay with recombinant protein antibodies, immunostaining, flow cytometry Proceedings of the National Academy of Sciences of the United States of America High 8855306
1997 The human DDOST gene encoding the 48-kDa OST subunit was characterized: organized into 11 exons spanning ~9 kb, localized to chromosome 1p36.1 by FISH, and expressed most intensely in heart and pancreas with lower levels in brain. Mouse and human DDOST cDNAs were isolated from retinoic acid-treated cells. cDNA cloning, genomic organization analysis, FISH chromosomal mapping, Northern blot expression analysis Genomics Medium 9367678
1998 OST48 (DDOST product) was identified as the 50-kDa subunit copurifying with ribophorins I and II in N-glycosylation activity purified from human lymphocyte microsomes; N-glycosylation activity increased 10-fold after mitogen activation of peripheral blood lymphocytes and remained elevated with IL-2 support, demonstrating that OST48-containing complex mediates ER N-glycosylation in lymphocytes and that this activity is induced during T-cell activation. Subcellular fractionation, enzyme purification (85-fold), N-glycosylation activity assay with synthetic acceptor peptide, N-terminal protein sequence analysis Biochemical and biophysical research communications Medium 9642163
2001 ER localization of OST48 (pig) is mediated by two mechanisms: (1) a C-terminal cytosolic dilysine motif (lysines at positions -3 and -5 from the C-terminus) that acts as a retrieval signal — replacing these with leucine allowed OST48 to reach the cell surface; and (2) complex formation with ribophorin I via luminal domain interactions, which retains OST48 in the ER independently of the dilysine motif. Site-directed mutagenesis of C-terminal lysines, immunofluorescence microscopy of OST48 mutants and hybrid proteins in COS-1 cells, co-expression with myc-tagged ribophorin I Biological chemistry High 11530934
2009 Using isoform-specific siRNA knockdowns, the STT3A-containing OST isoform (which includes OST48/DDOST as a shared subunit) was shown to be primarily responsible for cotranslational glycosylation of nascent polypeptides, while the STT3B isoform efficiently mediates posttranslational glycosylation of C-terminal acceptor sites in unfolded proteins; the two OST isoforms cooperate sequentially to maximize N-glycosylation efficiency. Isoform-specific siRNA knockdown, biosynthetic pulse labeling, glycosylation site-specific analysis Cell High 19167329
2012 siRNA-mediated knockdown of OST48 (DDOST product) or DAD1 destabilized both STT3A- and STT3B-containing OST complexes and caused pronounced hypoglycosylation, demonstrating that OST48 is required for the assembly and stability of both mammalian OST isoforms. In contrast, KCP2 knockdown produced a substrate-specific glycosylation defect and accumulated a novel STT3A-containing subcomplex, indicating OST48 plays a global structural role while KCP2 has a selective modulatory role. Subunit-specific siRNA knockdown, Western blot analysis of OST complex integrity, glycosylation phenotype analysis Journal of cell science High 22467853
2012 Whole-exome sequencing combined with glyco-complementation identified pathogenic mutations in DDOST (a 22-bp deletion and a missense mutation) in a congenital disorder of glycosylation (CDG) patient; N-glycosylation was decreased in patient fibroblasts as measured by three biomarkers, and complementation with wild-type DDOST cDNA restored glycosylation, establishing loss-of-function DDOST mutations as causative for CDG. Whole-exome sequencing, biochemical glycosylation assay (three biomarkers), glyco-complementation with wild-type DDOST cDNA in patient fibroblasts American journal of human genetics High 22305527
2012 Tandem affinity purification from mouse embryonic stem cells identified DDOST as a novel interacting protein of the testis-specific protein phosphatase isoform PPP1CC2; reciprocal in vitro sedimentation assays confirmed the DDOST-PPP1CC2 interaction, and immunolocalization showed DDOST localizes to the nuclear envelope in dissociated spermatogenic cells throughout spermatogenesis. Tandem affinity purification (TAP) from knock-in ESC lines, reciprocal in vitro sedimentation assay, immunolocalization in spermatogenic cells Biochemistry Medium 23140390
2013 Biosynthetic pulse labeling of five human glycoproteins demonstrated that extreme C-terminal glycosylation sites (within 50-55 residues of the C-terminus) are modified by a posttranslocational STT3B-dependent mechanism rather than by the STT3A-associated OST complex (which includes OST48/DDOST); NXT sites were glycosylated more rapidly and efficiently than NXS sites by this STT3B mechanism. Biosynthetic pulse labeling, isoform-specific siRNA knockdown, glycosylation site analysis, bioinformatics of glycopeptide databases The Journal of cell biology High 23530066
2015 Using SILAC-SRM mass spectrometry in yeast, ER-associated degradation (ERAD) was shown to compensate for imbalanced stoichiometry of OST subunits (including WBP1/OST48 ortholog) by degrading excess subunits; disrupted homeostasis in heterozygous diploid yeast cells was corrected by ERAD, indicating that protein degradation is a key mechanism maintaining OST complex assembly. SILAC coupled to selected reaction monitoring (SRM) mass spectrometry, yeast genetic tools, heterozygous diploid analysis Molecular biology of the cell Medium 25995378
2017 OST48 (DDOST product) overexpression in mice (DDOST+/- knock-in) combined with a high-AGE diet significantly exacerbated liver injury; DDOST overexpression increased portal delivery and hepatic accumulation of AGEs, leading to hepatic ER stress, oxidative stress, insulin secretory defects, altered fuel utilization, and hepatomegaly with glycogen accumulation, revealing a novel role of the OST48/AGE axis in hepatic injury through ER stress. Transgenic mouse model (DDOST+/- knock-in), dietary AGE manipulation, liver function tests, histology, metabolic phenotyping Scientific reports Medium 28947796
2019 Cryo-EM structures of human OST-A (STT3A-containing, includes OST48/DDOST) and OST-B (STT3B-containing, includes OST48/DDOST) were solved at high resolution; both complexes share similar overall architecture but differ in catalytic subunit contacts — DC2 associates with OST-A and MAGT1 with OST-B. In OST-A, ribophorin-I (shared with OST48) forms a four-helix bundle that contacts translating ribosomes, enabling cotranslational glycosylation, while this region is disordered in OST-B. Cryo-electron microscopy structural determination Science (New York, N.Y.) High 31831667
2021 Podocyte-specific overexpression of OST48 (DDOST product) in mice caused glomerular ER stress, foot process effacement, glomerular basement membrane thickening, collagen IV deposition, and tubulointerstitial fibrosis, with co-localization of OST48 and AGEs at podocyte foot processes; glomerular proteome was enriched for collagen deposition and oxidative stress pathways, indicating that OST48 overexpression in podocytes drives kidney injury via ER stress. Podocyte-specific transgenic mouse model (podocin promoter-driven DDOST), electron microscopy, histology, proteomics of isolated glomeruli Endocrinology, diabetes & metabolism Medium 34277994
2022 DDOST mediates N-glycosylation of MITA/STING in the ER upon DNA viral infection; selective mutation of DDOST-dependent N-glycosylation sites on MITA abolished MITA oligomerization and thereby its antiviral immune functions. Increasing Ddost expression in mouse brain enhanced local immune response to HSV-1 and prolonged survival in HSV encephalitis. Site-directed mutagenesis of MITA N-glycosylation sites, DDOST knockdown/overexpression, viral infection assays, mouse in vivo model of HSV encephalitis PLoS pathogens High 36449507
2023 Using proximity labeling, OST48 (DDOST product) was identified as a protein that transiently interacts with lysyl oxidase (LOX), a secreted enzyme; an assay based on LOX N-glycosylation was developed to functionally test DDOST variants of uncertain significance, and two variants (p.S243F and p.E286del) were demonstrated to be functionally impaired in OST glycosylation activity. Proximity labeling (BioID), LOX N-glycosylation functional assay, variant testing of 41 DDOST VUS Scientific reports Medium 37848450
2024 DDOST knockdown in pancreatic ductal adenocarcinoma (PDAC) cell lines decreased cell proliferation and viability while increasing ER stress, ROS formation, and apoptosis; quantitative mass spectrometry identified 30 differentially expressed proteins/phosphopeptides after DDOST knockdown, with protein-protein interaction network analysis supporting an oncogenic role of DDOST in PDAC by intercepting cell stress pathways to reduce apoptosis. siRNA knockdown, quantitative mass spectrometry, cell viability assays, apoptosis assays, ROS measurement, ER stress markers Scientific reports Medium 39223141
2025 DDOST depletion in hepatocellular carcinoma cells impaired EGFR N-glycosylation, suppressing downstream AKT, ERK5, and ERK1/2 signaling and sensitizing cells to lenvatinib; DDOST loss also reduced PD-L1 glycosylation. The OST inhibitor NGI-1 and NGI-1-loaded nanoparticles showed antitumor effects and augmented efficacy of lenvatinib and immunotherapy in vivo. DDOST knockdown, EGFR/PD-L1 glycosylation analysis, Western blot for signaling pathway components, in vitro and in vivo xenograft assays, OST inhibitor treatment Experimental & molecular medicine Medium 41413687
2025 Macrophage-derived exosomal miR-146a-5p targets and suppresses PNKP expression, which reduces PNKP-DDOST interaction and enhances DDOST phosphorylation; this activated JAGN1-dependent NET formation in neutrophils in an atherosclerosis model, identifying DDOST as subject to phosphorylation-dependent regulation that controls its interaction with PNKP. Co-immunoprecipitation, RIP, dual-luciferase reporter assay, Western blot, qRT-PCR, in vivo ApoE-/- mouse atherosclerosis model Cellular signalling Medium 41109654

Source papers

Stage 0 corpus · 90 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2009 Cotranslational and posttranslational N-glycosylation of polypeptides by distinct mammalian OST isoforms. Cell 330 19167329
1996 Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proceedings of the National Academy of Sciences of the United States of America 299 8855306
1994 A novel oncogene, ost, encodes a guanine nucleotide exchange factor that potentially links Rho and Rac signaling pathways. The EMBO journal 202 7957046
2019 Cryo-electron microscopy structures of human oligosaccharyltransferase complexes OST-A and OST-B. Science (New York, N.Y.) 118 31831667
1992 The yeast WBP1 is essential for oligosaccharyl transferase activity in vivo and in vitro. The EMBO journal 116 1600939
2003 Methylation-associated silencing of heparan sulfate D-glucosaminyl 3-O-sulfotransferase-2 (3-OST-2) in human breast, colon, lung and pancreatic cancers. Oncogene 115 12527896
1995 Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1. DNA research : an international journal for rapid publication of reports on genes and genomes 92 7788527
2013 Extreme C-terminal sites are posttranslocationally glycosylated by the STT3B isoform of the OST. The Journal of cell biology 83 23530066
2012 The oligosaccharyltransferase subunits OST48, DAD1 and KCP2 function as ubiquitous and selective modulators of mammalian N-glycosylation. Journal of cell science 77 22467853
1992 The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1. The Journal of biological chemistry 69 1429707
2010 Getting the mOST from OST: Role of organic solute transporter, OSTalpha-OSTbeta, in bile acid and steroid metabolism. Biochimica et biophysica acta 68 20538072
2010 OST-HTH: a novel predicted RNA-binding domain. Biology direct 66 20302647
2012 DDOST mutations identified by whole-exome sequencing are implicated in congenital disorders of glycosylation. American journal of human genetics 57 22305527
2017 Selenoprotein T is a novel OST subunit that regulates UPR signaling and hormone secretion. EMBO reports 50 28928140
2015 Protein degradation corrects for imbalanced subunit stoichiometry in OST complex assembly. Molecular biology of the cell 46 25995378
2007 Heterodimerization, trafficking and membrane topology of the two proteins, Ost alpha and Ost beta, that constitute the organic solute and steroid transporter. The Biochemical journal 45 17650074
2012 Biophysical basis of the binding of WWOX tumor suppressor to WBP1 and WBP2 adaptors. Journal of molecular biology 35 22634283
2011 Biophysical analysis of binding of WW domains of the YAP2 transcriptional regulator to PPXY motifs within WBP1 and WBP2 adaptors. Biochemistry 34 21981024
1999 De novo deletions of p53 gene and wild-type p53 correlate with acquired cisplatin-resistance in human osteosarcoma OST cell line. Anticancer research 30 10697522
1997 Genome organization of human 48-kDa oligosaccharyltransferase (DDOST). Genomics 27 9367678
2006 An orally active cathepsin K inhibitor, furan-2-carboxylic acid, 1-{1-[4-fluoro-2-(2-oxo-pyrrolidin-1-yl)-phenyl]-3-oxo-piperidin-4-ylcarbamoyl}-cyclohexyl)-amide (OST-4077), inhibits osteoclast activity in vitro and bone loss in ovariectomized rats. The Journal of pharmacology and experimental therapeutics 25 16699068
2017 Increased liver AGEs induce hepatic injury mediated through an OST48 pathway. Scientific reports 24 28947796
2006 A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene. Protein expression and purification 24 16769222
1995 Human oligosaccharyltransferase: isolation, characterization, and the complete amino acid sequence of 50-kDa subunit. Archives of biochemistry and biophysics 23 7625827
1994 The genetic interaction of kar2 and wbp1 mutations. Distinct functions of binding protein BiP and N-linked glycosylation in the processing pathway of secreted proteins in Saccharomyces cerevisiae. European journal of biochemistry 23 8020500
1999 Distinct expression patterns and transforming properties of multiple isoforms of Ost, an exchange factor for RhoA and Cdc42. Oncogene 22 10467422
1996 Parathyroid hormone regulates the expression of the receptor protein tyrosine phosphatase, OST-PTP, in rat osteoblast-like cells. Endocrinology 22 8603605
2001 Tumour necrosis factor-alpha provokes upregulation of alpha2beta1 and alpha5beta1 integrins, and cell migration in OST osteosarcoma cells. Cell biology international 20 11319838
2022 DDOST Correlated with Malignancies and Immune Microenvironment in Gliomas. Frontiers in immunology 18 35812432
2020 Osthole-loaded N-octyl-O-sulfonyl chitosan micelles (NSC-OST) inhibits RANKL-induced osteoclastogenesis and prevents ovariectomy-induced bone loss in rats. Journal of cellular and molecular medicine 18 32126148
2013 3-OST-7 regulates BMP-dependent cardiac contraction. PLoS biology 18 24311987
2024 A novel antimicrobial peptide WBp-1 from wheat bran: Purification, characterization and antibacterial potential against Listeria monocytogenes. Food chemistry 16 39321596
2017 Characteristics of candidate genes associated with embryonic development in the cow: Evidence for a role for WBP1 in development to the blastocyst stage. PloS one 16 28542629
2011 Ultrastructural and mineral phase characterization of the bone-like matrix assembled in F-OST osteoblast cultures. Calcified tissue international 16 21901516
1996 Human osteosarcoma (OST) induces mouse reactive bone formation in xenograft system. Bone 16 8922642
2017 Dietary advanced glycated end-products and medicines influence the expression of SIRT1 and DDOST in peripheral mononuclear cells from long-term type 1 diabetes patients. Diabetes & vascular disease research 15 29027826
2009 Screening for OST deficiencies in unsolved CDG-I patients. Biochemical and biophysical research communications 14 19835842
2012 Tandem affinity purification in transgenic mouse embryonic stem cells identifies DDOST as a novel PPP1CC2 interacting protein. Biochemistry 13 23140390
2002 Transcriptional activation of the tyrosine phosphatase gene, OST-PTP, during osteoblast differentiation. Journal of cellular biochemistry 12 12397596
2008 Arachidonic acid-induced expression of the organic solute and steroid transporter-beta (Ost-beta) in a cartilaginous fish cell line. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 11 18407792
2007 Role of the guanine nucleotide exchange factor Ost in negative regulation of receptor endocytosis by the small GTPase Rac1. The Journal of biological chemistry 11 17562712
1998 Interleukin-2 induces N-glycosylation in T-cells: characterization of human lymphocyte oligosaccharyltransferase. Biochemical and biophysical research communications 11 9642163
2022 MITA oligomerization upon viral infection is dependent on its N-glycosylation mediated by DDOST. PLoS pathogens 10 36449507
2025 The solute carrier superfamily interactome. Molecular systems biology 9 40355756
2013 Members of 3-O-Sulfotransferases (3-OST) Family: A Valuable Tool from Zebrafish to Humans for Understanding Herpes Simplex Virus Entry. The open virology journal 9 23358893
2004 The tyrosine phosphatase, OST-PTP, is expressed in mesenchymal progenitor cells early during skeletogenesis in the mouse. Journal of cellular biochemistry 9 15660420
2022 ost in promiscuity? An evolutionary and biochemical evaluation of HSD10 function in cardiolipin metabolism. Cellular and molecular life sciences : CMLS 8 36271951
2021 Thioxothiazolidin derivative, 4-OST, inhibits melanogenesis by enhancing the specific recruitment of tyrosinase-containing vesicles to lysosome. Journal of cellular biochemistry 8 33480093
2023 LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR-532-3p/DDOST axis. Journal of biochemical and molecular toxicology 7 37155292
2022 DDOST-CDG: Clinical and molecular characterization of a third patient with a milder and a predominantly movement disorder phenotype. Journal of inherited metabolic disease 7 36214423
2021 The AGE receptor, OST48 drives podocyte foot process effacement and basement membrane expansion (alters structural composition). Endocrinology, diabetes & metabolism 7 34277994
2017 Transmembrane motions of PglB induced by LLO are coupled with EL5 loop conformational changes necessary for OST activity. Glycobiology 7 28575441
2013 Homologue gene of bile acid transporters ntcp, asbt, and ost-alpha in rainbow trout Oncorhynchus mykiss: tissue expression, effect of fasting, and response to bile acid administration. Fish physiology and biochemistry 7 24026769
2025 EndoMAP.v1 charts the structural landscape of human early endosome complexes. Nature 6 40437099
2019 The OST-complex as target for RNAi-based pest control in Nilaparvata lugens. Archives of insect biochemistry and physiology 6 31038785
2001 Analysis of structural signals conferring localisation of pig OST48 to the endoplasmic reticulum. Biological chemistry 6 11530934
1995 PCR-mediated cloning and sequencing of the DmOST50 gene, a WBP1/AvOST50/OST48 homologue, from Drosophila melanogaster. Gene 6 7607543
2024 Associations between the VDR Gene rs731236 (TaqI) Polymorphism and Bone Mineral Density in Postmenopausal Women from the RAC-OST-POL. Biomedicines 5 38672272
2021 The second DDOST-CDG patient with lactose intolerance, developmental delay, and situs inversus totalis. Journal of human genetics 5 34462534
2017 Rebuttal of Atkins et al. (2017) critique of the Öst (2014) meta-analysis of ACT. Behaviour research and therapy 5 28911853
2025 WBP1 regulates mitochondrial function and ferroptosis to modulate chemoresistance in colorectal cancer. Molecular medicine (Cambridge, Mass.) 4 40075333
2025 Redundancy of the OST catalytic subunit facilitates therapeutic targeting of N-glycosylation. Cell chemical biology 4 40494352
2024 Induction of oxidative- and endoplasmic-reticulum-stress dependent apoptosis in pancreatic cancer cell lines by DDOST knockdown. Scientific reports 4 39223141
2023 Functional classification of DDOST variants of uncertain clinical significance in congenital disorders of glycosylation. Scientific reports 4 37848450
2019 Globally elevating the AGE clearance receptor, OST48, does not protect against the development of diabetic kidney disease, despite improving insulin secretion. Scientific reports 4 31541173
2025 Pharmacological activity of OST-01, a natural product from baccharis coridifolia, on breast cancer cells. Journal of hematology & oncology 3 39920848
2025 Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids. Nature communications 2 40593736
2025 Engineered nanovesicle platform simultaneously triggers YAP-dependent ferroptosis and reprograms T-cell immunity through miR-150-3p codelivery in melanoma microenvironment. Theranostics 2 40860143
2022 Site-to-site cross-talk in OST-B glycosylation of hCEACAM1-IgV. Proceedings of the National Academy of Sciences of the United States of America 2 36251991
2025 Interaction Proteomics of Polycystins 1 and 2 Reveal a Novel Role for the BLOC-1/BORC Lysosomal Positioning Complex. Molecular & cellular proteomics : MCP 1 41086943
2025 Macrophage-derived exosome miR-146a-5p modulates PNKP/DDOST/JAGN1 complex to regulate NETs formation in atherosclerosis. Cellular signalling 1 41109654
2025 Knockdown of SUCLG2 inhibits glioblastoma proliferation and promotes apoptosis through LMNA acetylation and the mediation of H4K16la lactylation. Cell death discovery 1 41249152
2024 DDOST is associated with tumor immunosuppressive microenvironment in cervical cancer. Discover oncology 1 38460058
2024 Bone Fracture Incidence in Postmenopausal Women: Results of a 10 Year Follow Up in a RAC-OST-POL Study of rs1544410, rs7975232 and rs731236 Polymorphisms. Nutrients 1 39683539
2001 Ostip2, a novel oncoprotein that associates with the Rho exchange factor Ost. DNA and cell biology 1 11506702
1992 Genetic and physical mapping of the WBP1 locus close to CENV. Yeast (Chichester, England) 1 1293887
2026 Differentiation-dependent proximity proteomics identifies novel host factors linked to HPV16 E2 function. mBio 0 41524403
2026 Oligosaccharyltransferase (OST) complex inhibition effectively treats rodent and human prions. PLoS pathogens 0 41525337
2026 Differential contributions of human oligosaccharyltransferase complexes OST-A and OST-B to HIV-1 envelope glycoprotein glycosylation. Journal of virology 0 41778789
2026 Effects and mechanisms of dietary Osthole (OST) and Tetramethylpyrazine (TMP) on growth performance, antioxidant capability and immune response of Pacific white shrimp (Litopenaeus vannamei). The British journal of nutrition 0 41879046
2026 Temporal Dynamics and Uptake Mechanisms of Carbonated Hydroxyapatite Nanoparticles in Murine F‑OST Cells. ACS omega 0 41939395
2025 Progress in research on DDOST dysregulation in related diseases. Glycoconjugate journal 0 40601285
2025 E3 ligase AREL1 controls perinuclear localization of lysosomes and supports Purkinje cell survival. The EMBO journal 0 41331534
2025 DDOST-Congenital Disorder of Glycosylation: Defining the Clinical Spectrum and First Report of a Structural Variant. American journal of medical genetics. Part A 0 41392699
2025 Targeting DDOST improves the efficacy of lenvatinib and immunotherapy in hepatocellular carcinoma. Experimental & molecular medicine 0 41413687
2025 Integrated transcriptomic and proteomic analysis reveals the mechanistic role of OST in cutaneous squamous cell carcinoma. Neoplasma 0 41567021
2024 Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR. Journal of biomolecular NMR 0 38421550
2024 OST Catalytic Subunit Redundancy Enables Therapeutic Targeting of N-Glycosylation. bioRxiv : the preprint server for biology 0 39677793
2020 Inflammatory conditions promote a switch of oligosaccharyltransferase (OST) catalytic subunit isoform expression. Archives of biochemistry and biophysics 0 32810478
1999 Influence of antiresorptive agent OST-766 on signal transduction pathways involved in parathyroid hormone action. Calcified tissue international 0 9914323