Affinage

RILP

Rab-interacting lysosomal protein · UniProt Q96NA2

Length
401 aa
Mass
44.2 kDa
Annotated
2026-04-28
61 papers in source corpus 31 papers cited in narrative 30 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RILP is a coiled-coil effector of Rab7 and related GTPases (Rab34, Rab36, Rab12) that serves as a central adaptor coupling late endosomal/lysosomal identity to dynein-dynactin-dependent minus-end microtubule transport, organelle tethering, multivesicular body biogenesis, and autophagy (PMID:11179213, PMID:11696325, PMID:23729732, PMID:32275887). Recruited to endolysosomal membranes by GTP-bound Rab7, RILP directly binds the p150Glued subunit of dynactin and simultaneously engages the HOPS tethering complex, linking retrograde transport to homotypic fusion, while its interaction with ESCRT-II subunits Vps22/Vps36 regulates intralumenal vesicle formation and EGFR degradation (PMID:17283181, PMID:17959629, PMID:23729732). RILP activity is tuned by cholesterol-sensing through ORP1L, which under low-cholesterol conditions promotes ER–late endosome contacts via VAP that strip dynein from the RILP complex, by LRRK1-mediated Rab7 S72 phosphorylation that enhances RILP recruitment, and by proteolytic cleavage at D75 by caspase-1 or viral proteases that redirect vesicular trafficking peripherally (PMID:19564404, PMID:31085713, PMID:27791088, PMID:30100068). Beyond canonical endolysosomal trafficking, RILP controls phagosome maturation, autophagosome biogenesis through interaction with ATG5 and LC3, melanosome and mast cell granule positioning, and V-ATPase assembly via regulation of V1G1 subunit stability (PMID:12944476, PMID:32275887, PMID:22740695, PMID:26740112, PMID:24762812).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2001 High

    Identification of RILP as a Rab7-GTP-specific effector that recruits dynein-dynactin to late endosomes/lysosomes established the first molecular link between Rab7 signaling and minus-end-directed endosomal transport.

    Evidence Yeast two-hybrid, co-IP, GST pulldown, overexpression/dominant-negative constructs, degradation assays in mammalian cells

    PMID:11179213 PMID:11696325

    Open questions at the time
    • Identity of the dynactin subunit directly contacted by RILP was not yet mapped
    • Whether RILP had functions beyond transport (e.g., fusion, MVB biogenesis) was unknown
  2. 2003 High

    Demonstrating that RILP is essential for phagosome maturation via dynein-dynactin-dependent tubule extension extended its function beyond canonical endosome transport to innate immunity, while domain mapping identified a 62-residue Rab-binding region also recognizing Rab34.

    Evidence RILP truncation mutants in phagosome maturation assays, electron microscopy, domain-swapping chimeras with RLP1

    PMID:12944476 PMID:14668488

    Open questions at the time
    • Whether RILP-Rab34 interaction had independent physiological relevance was unclear
    • Structural basis for dual Rab recognition unknown
  3. 2004 High

    Discovery that Salmonella effector SifA disrupts the Rab7-RILP interaction to redirect vacuolar trafficking demonstrated that pathogens exploit the RILP-dynein axis as a vulnerability point in host defense.

    Evidence Immobilized RILP pulldown, immunofluorescence, SifA epistasis in infected cells

    PMID:15121880

    Open questions at the time
    • Molecular mechanism by which SifA dissociates Rab7-RILP not resolved
    • Generalizability to other intracellular pathogens not yet established
  4. 2007 High

    Mapping RILP's direct contact to the p150Glued C-terminal domain and discovering the tripartite Rab7-RILP-ORP1L complex, together with RILP's interaction with ESCRT-II subunits Vps22/Vps36 for MVB biogenesis, revealed RILP as a multifunctional scaffold integrating transport, tethering, and cargo sorting.

    Evidence Co-IP, GST pulldown, siRNA, EM for intralumenal vesicles, EGFR degradation assays

    PMID:16857164 PMID:17283181 PMID:17959629

    Open questions at the time
    • How RILP coordinates simultaneous binding to dynactin, ESCRT-II, and HOPS was structurally unresolved
    • Stoichiometry of the tripartite complex unknown
  5. 2009 High

    The finding that ORP1L senses late endosomal cholesterol levels and, under low cholesterol, triggers ER-LE contacts via VAP that strip dynein from RILP established a lipid-sensing regulatory switch controlling RILP-dependent transport direction.

    Evidence Cholesterol manipulation, co-IP, dominant-negative constructs, fluorescence microscopy

    PMID:19564404

    Open questions at the time
    • Structural basis for VAP-mediated p150Glued displacement not determined
    • Whether cholesterol regulation of RILP occurs in all cell types unknown
  6. 2012 High

    Identification of Rab36 and melanoregulin as RILP partners for retrograde melanosome transport demonstrated that RILP functions as a shared dynein adaptor across multiple Rab GTPase pathways and organelle types beyond classical late endosomes.

    Evidence Yeast two-hybrid, site-directed mutagenesis of RHD, melanosome distribution assays in melanocytes, co-IP with p150Glued and melanoregulin

    PMID:22275436 PMID:22740695

    Open questions at the time
    • Whether Rab36 and Rab7 compete for the same RILP dimer interface was not resolved
    • Physiological relevance of melanoregulin-RILP outside melanocytes unclear
  7. 2013 High

    Showing that RILP simultaneously binds HOPS tethering complex and p150Glued linked transport and fusion into a single module, explaining how late endosomes couple motility with homotypic tethering competence.

    Evidence Co-IP, siRNA, haploid cell genetic screen, Ebola infection assay, in vitro binding

    PMID:23729732

    Open questions at the time
    • Which HOPS subunit(s) directly contact RILP was not mapped
    • Whether RILP-HOPS interaction is cholesterol-regulated like RILP-dynein was not fully dissected
  8. 2014 High

    The discovery that RILP binds and promotes proteasomal degradation of V-ATPase subunit V1G1 revealed an unexpected role in regulating endolysosomal acidification, extending RILP function beyond transport to organelle homeostasis.

    Evidence Co-IP, V-ATPase activity assay, ubiquitylation assay, siRNA knockdown

    PMID:24762812

    Open questions at the time
    • E3 ligase mediating V1G1 ubiquitylation downstream of RILP was not identified
    • How V1G1-RILP and Rab7-RILP interactions are coordinated structurally unknown
  9. 2016 High

    Three discoveries — FLCN loading Rab34 onto RILP, Rab12-RILP controlling mast cell granule transport, and HCV NS3/4A protease cleaving RILP to redirect trafficking — established RILP as a convergence point for diverse upstream Rab signals and a target for pathogen-mediated subversion via proteolysis.

    Evidence In vitro reconstitution with purified FLCN-DENN/RILP, co-IP of Rab12, viral cleavage western blots, degranulation and virion secretion assays

    PMID:26740112 PMID:27113757 PMID:27791088

    Open questions at the time
    • Whether FLCN-Rab34-RILP and Rab7-RILP complexes coexist or are mutually exclusive unknown
    • Precise cleavage site for NS3/4A on RILP not mapped at residue level
  10. 2018 High

    Crystal structure of Rab7-ORP1L revealed that ORP1L binds a non-canonical Rab7 surface, leaving switch regions free for RILP, structurally explaining simultaneous tripartite complex formation; caspase-1 cleavage at D75 and associated phosphorylation were shown to cooperatively release RILP from dynein.

    Evidence X-ray crystallography of Rab7-ORP1L, mutagenesis, in vitro caspase-1 cleavage assay with D75A mutant

    PMID:30012887 PMID:30100068

    Open questions at the time
    • Full atomic structure of the Rab7-RILP-ORP1L ternary complex not yet solved
    • Kinase responsible for phosphorylation near D75 not identified
  11. 2019 High

    LRRK1 phosphorylation of Rab7 at S72 was shown to promote RILP binding and consequent dynein-mediated transport, establishing a kinase-dependent regulatory input upstream of the Rab7-RILP axis relevant to EGFR downregulation.

    Evidence In vitro kinase assay, phospho-specific antibody, co-IP, endosome transport assays with LRRK1 knockdown

    PMID:31085713

    Open questions at the time
    • Phosphatase counteracting S72 phosphorylation not identified
    • Whether LRRK2 (Parkinson's-linked paralog) similarly regulates RILP binding unknown
  12. 2020 High

    Discovery that RILP interacts with ATG5 on isolation membranes and with LC3 via LIR motifs, controlling autophagosome biogenesis and subsequent dynein-mediated autophagosome transport, unified RILP's roles in degradative trafficking and autophagy.

    Evidence siRNA knockdown, LIR motif mutagenesis, co-IP with ATG5/LC3, autophagy flux assays in neurons

    PMID:32275887

    Open questions at the time
    • Whether RILP-ATG5 interaction inhibits or scaffolds phagophore closure not distinguished
    • Regulation of RILP expression by mTOR pathway not mechanistically resolved
  13. 2024 Medium

    Multiple 2024 studies integrated RILP into broader regulatory circuits: DENND6A as an Arl8b effector activates Rab34 to recruit RILP/dynein for lysosome repositioning; a V-ATPase-RILP-Rab7 feedback loop controls endosomal pH; RILP-ORP1L competition with VAP regulates cholesterol transfer and autophagy; and Rab7 Y183 phosphorylation recruits RILP to lipid droplets for microlipophagy.

    Evidence GEF screen, epistasis, co-IP, lysosome/endosome positioning assays, pH manipulation, cholesterol assays, conditional knockout mice with pharmacological rescue

    PMID:38296963 PMID:38578235 PMID:38837607 PMID:39195203

    Open questions at the time
    • Whether DENND6A-Rab34-RILP and Rab7-RILP represent parallel or sequential pathways on the same organelle unclear
    • Kinase mediating Rab7 Y183 phosphorylation not identified
    • In vivo validation of RILP's role in lipid droplet degradation limited to one disease model

Open questions

Synthesis pass · forward-looking unresolved questions
  • No high-resolution structure of the full-length RILP homodimer in complex with Rab7, dynactin p150Glued, and HOPS exists; how RILP coordinates its multiple binding partners simultaneously on the same membrane domain, and how cell-type-specific Rab inputs (Rab7, Rab34, Rab36, Rab12, Rab26) are prioritized, remain open questions.
  • No full-length RILP structure available
  • Stoichiometry and mutual exclusivity of RILP's simultaneous binding partners unresolved
  • Tissue-specific phenotypes of RILP loss in vivo not characterized in knockout animal models

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 5 GO:0008092 cytoskeletal protein binding 3
Localization
GO:0005768 endosome 5 GO:0005764 lysosome 4 GO:0031410 cytoplasmic vesicle 3 GO:0005829 cytosol 1
Pathway
R-HSA-5653656 Vesicle-mediated transport 6 R-HSA-168256 Immune System 3 R-HSA-9612973 Autophagy 3
Partners
ATP6V1G1DCTN1FLCNORP1LRAB34RAB7AVPS22VPS36
Complex memberships
RILP-HOPS tethering complexRILP-dynein-dynactin motor complexRab7-RILP-ORP1L tripartite complex

Evidence

Reading pass · 30 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 RILP (Rab7-interacting lysosomal protein) specifically binds GTP-bound Rab7 and recruits functional dynein-dynactin motor complexes to late endosomes and lysosomes, causing their transport toward microtubule minus ends and inhibiting peripheral trafficking. Co-immunoprecipitation, overexpression in mammalian cells, fluorescence microscopy, dominant-negative constructs Current Biology High 11696325
2001 RILP is a 45 kDa protein containing two coiled-coil regions, found mainly in cytosol, that is recruited to late endosomal/lysosomal membranes by Rab7-GTP via its C-terminus; RILP-C33 (truncated C-terminal form lacking N-terminal half) acts as dominant negative, inhibiting EGF and LDL degradation and dispersing lysosomes similarly to dominant-negative Rab7. Yeast two-hybrid, GST pulldown, overexpression of truncation mutants, degradation assays The EMBO Journal High 11179213
2003 RILP promotes extension of phagosomal tubules toward late endocytic compartments by recruiting dynein-dynactin; a truncated RILP lacking the dynein-dynactin-recruiting domain prevents tubule extension and phagosome-lysosome fusion, establishing RILP as essential for phagosome maturation. Fluorescence microscopy, electron microscopy, overexpression of RILP truncation mutants, functional phagosome maturation assays Molecular and Cellular Biology High 12944476
2003 A unique 62-residue region (amino acids 272–333) in RILP is necessary and sufficient for regulating lysosomal morphology and for interaction with GTP-bound Rab7 and Rab34; transferring this region into the related protein RLP1 confers lysosome-regulatory activity. Domain swapping/chimeric protein expression, GTPase interaction assays, lysosome morphology readout in mammalian cells Molecular Biology of the Cell High 14668488
2004 Salmonella effector SifA disrupts the Rab7–RILP interaction on Salmonella-containing vacuoles, preventing dynein recruitment; this uncoupling allows kinesin-dependent centrifugal extension of Salmonella-induced filaments, promoting bacterial replication in a protected compartment. Co-transfection, immunofluorescence, immobilized RILP pulldown of active Rab7, cell-free system with BCG supernatant Molecular Biology of the Cell High 15121880
2007 GTP-bound Rab7 simultaneously binds RILP and ORP1L to form a tripartite RILP-Rab7-ORP1L complex; RILP directly contacts the C-terminal 25-kDa region of dynactin p150Glued; ORP1L and betaIII spectrin are additionally required for dynein motor activity, establishing a stepwise assembly cascade for minus-end endosomal transport. Co-immunoprecipitation, GST pulldown, siRNA knockdown, live-cell imaging, dominant-negative constructs The Journal of Cell Biology High 17283181
2007 RILP is required for biogenesis of multivesicular endosomes (MVEs): RILP depletion by siRNA reduces intralumenal vesicle content, impairs EGFR degradation (but not transferrin recycling), and causes elevated levels of late-endosomal markers. RILP interacts with ESCRT-II subunits Vps22 and Vps36. siRNA knockdown, electron microscopy, degradation assays for EGFR vs. transferrin, co-immunoprecipitation with ESCRT-II subunits Journal of Cell Science High 17959629
2006 RILP interacts with VPS22 (EAP30/SNF8) of ESCRT-II via its N-terminal half, and with VPS36 (EAP45) via its C-terminal half; RILP overexpression causes enlarged, clustered MVBs and retards EGF sorting at sorting endosomes, suggesting a regulatory loop between early and late endocytic machinery. Yeast two-hybrid, co-immunoprecipitation, confocal immunofluorescence, RILP domain truncation analysis Biochemical and Biophysical Research Communications Medium 16857164 17010938
2007 Mycobacterium bovis BCG inhibits phagosome maturation by preventing RILP recruitment to Rab7 on phagosomal membranes; BCG culture supernatant contains a factor that catalyzes GTP→GDP conversion on Rab7, maintaining Rab7 in inactive GDP-bound form and blocking RILP-mediated lysosomal fusion. Co-transfection, immobilized RILP pulldown of GTP-bound Rab7, cell-free GTPase activity assay Journal of Leukocyte Biology Medium 18040083
2008 Huntingtin regulates REST/NRSF nuclear trafficking indirectly through a complex containing REST/NRSF, RILP, dynactin p150Glued, huntingtin, and HAP1; RILP directly binds p150Glued and REST/NRSF; mutant huntingtin weakens the dynactin p150Glued–RILP interaction; HAP1 prevents the complex from translocating REST/NRSF to the nucleus. Yeast two-hybrid, co-immunoprecipitation of in vitro translated proteins, complex characterization The Journal of Biological Chemistry Medium 18922795
2009 Cholesterol levels in late endosomes are sensed by ORP1L; under low cholesterol, ORP1L conformation induces formation of ER-late endosome membrane contact sites where VAP (ER protein) interacts in trans with the Rab7-RILP complex to remove p150Glued and associated motors, causing plus-end directed movement. High cholesterol (as in Niemann-Pick C) prevents this, locking LEs at minus-end via dynein-RILP. Co-immunoprecipitation, dominant-negative constructs, fluorescence microscopy, fractionation, cholesterol manipulation The Journal of Cell Biology High 19564404
2012 RILP functions as an effector of Rab36 (in addition to Rab7) via its RILP homology domain (RHD, a coiled-coil domain); site-directed mutagenesis of RHD revealed differential amino acid contributions to Rab7 vs. Rab36 binding; Rab36-RILP interaction mediates retrograde melanosome transport in melanocytes, independent of Rab7. Yeast two-hybrid screen, GST pulldown, site-directed mutagenesis, knockdown in melanocytes, melanosome distribution assays The Journal of Biological Chemistry High 22740695
2012 Melanoregulin (Mreg) interacts with the C-terminal domain of RILP and forms a trimeric complex with RILP and p150Glued, mediating dynein-dynactin-dependent retrograde melanosome transport; Mreg knockdown or dynein-dynactin disruption restores peripheral melanosome distribution in Rab27A-deficient melanocytes. Co-immunoprecipitation, siRNA knockdown, overexpression, melanosome distribution assays Journal of Cell Science Medium 22275436
2013 RILP simultaneously and directly binds both the HOPS tethering complex and p150Glued subunit of dynein-dynactin, coupling late endosomal transport and tethering into a single RAB7-RILP-ORP1L multiprotein complex; ORP1L acts as a cholesterol-sensing switch controlling RILP-HOPS-p150Glued interactions. Co-immunoprecipitation, siRNA knockdown, genetic epistasis (haploid cell screen), Ebola infection assay, in vitro binding Journal of Cell Science High 23729732
2014 RILP interacts with the V1G1 subunit (ATP6V1G1) of vacuolar ATPase, controls V1G1 recruitment to late endosomal/lysosomal membranes, promotes proteasomal degradation of V1G1 (via ubiquitylation), and thereby regulates V-ATPase assembly and activity. Co-immunoprecipitation, siRNA knockdown, V-ATPase activity assay, ubiquitylation assay, fluorescence microscopy Journal of Cell Science High 24762812
2015 RILP suppresses breast cancer cell invasion by interacting with RalGDS (Ral guanine nucleotide dissociation stimulator) via its N-terminal region; this interaction recruits RalGDS to late endosomes, inhibiting its GEF activity toward RalA and thereby suppressing invasion. Co-immunoprecipitation, truncation analysis, immunofluorescence, RalA activity assay, siRNA knockdown, migration/invasion assays Cell Death & Disease Medium 26469971
2016 Folliculin (FLCN) promotes perinuclear lysosome clustering by interacting directly via its C-terminal DENN domain with RILP; purified FLCN-DENN domain loads active Rab34 onto RILP (but does not act as a GEF for Rab34); this drives formation of Rab34-positive membrane contacts with lysosomes reducing their motility. Purified recombinant protein in vitro binding assay, siRNA knockdown, live-cell imaging, co-immunoprecipitation EMBO Reports High 27113757
2016 HCV (and Sendai virus) infection causes NS3/4A protease-dependent cleavage of RILP, generating a C-terminal fragment (cRILP) that lacks the N-terminus; cRILP redistributes to the cell periphery, releases from dynein p150Glued, and redirects Rab7 vesicles to kinesin-dependent trafficking, promoting virion secretion. Viral infection, western blot for RILP cleavage, siRNA knockdown, cRILP expression, kinesin inhibitor, trafficking assays Proceedings of the National Academy of Sciences High 27791088
2016 Rab12 is a novel binding partner and cargo of RILP; activated Rab12 interacts with RILP to mediate microtubule-dependent retrograde transport of mast cell secretory granules via the RILP-dynein complex; Rab12 negatively regulates mast cell degranulation through this mechanism. Co-immunoprecipitation, siRNA knockdown, live-cell imaging, dominant-negative constructs, degranulation assays Journal of Immunology Medium 26740112
2018 Caspase-1 directly cleaves RILP at aspartic acid 75; alanine substitution at D75 blocks caspase-1-mediated cleavage; redistribution of cleaved RILP to the cytoplasm requires both cleavage and specific phosphorylation events near the caspase-1 site; combined cleavage + phosphorylation are required for release from dynein p150Glued and redistribution of CD63-positive vesicles. In vitro caspase-1 cleavage assay, site-directed mutagenesis (D75A), phosphorylation analysis, localization by fluorescence microscopy Biochemical and Biophysical Research Communications Medium 30100068
2018 Rab7 interacts with ORP1L via a non-canonical site (helix3 and 310-helix2 of Rab7), independently of GTP/GDP state; this leaves the canonical effector-interacting switch regions free for RILP binding, enabling simultaneous ORP1L-Rab7-RILP tripartite complex formation; mutational disruption of the ORP1L-Rab7 interface impairs late endosome positioning. Crystal structure of Rab7-ORP1L ARDN, biochemical binding assays, mutagenesis, late endosome positioning assay The Journal of Biological Chemistry High 30012887
2019 LRRK1 phosphorylates GTP-bound Rab7 at serine 72 at endosomal membranes; this phosphorylation promotes RILP interaction with Rab7, leading to dynein-dynactin recruitment and dynein-driven transport of EGFR-containing endosomes to the perinuclear region. In vitro kinase assay, phospho-specific antibody, co-immunoprecipitation, LRRK1 knockdown/overexpression, endosome transport assay Journal of Cell Science High 31085713
2019 RILP interacts with insulin granule-associated Rab26 and mediates lysosomal degradation of proinsulin; RILP overexpression induces insulin granule clustering and promotes Rab7-dependent, lysosomal inhibitor-sensitive proinsulin degradation, thereby restricting insulin secretion. Co-immunoprecipitation, overexpression, siRNA knockdown, lysosomal inhibitor treatment, insulin secretion assays in beta-cell lines and islets Diabetes Medium 31624142
2020 RILP functions as a dynein adaptor for neuronal autophagosomes and controls autophagosome biogenesis: mTOR inhibition upregulates RILP expression and its localization to autophagosomes; RILP interacts with ATG5 on isolation membranes (preventing premature dynein recruitment) and with LC3 via LIR motifs; RILP depletion or LIR motif mutation strongly reduces autophagosome numbers and impairs autophagic turnover. siRNA knockdown, LIR motif mutagenesis, co-immunoprecipitation with ATG5 and LC3, live-cell imaging, autophagy flux assays Developmental Cell High 32275887
2021 Biochemical and in silico analysis reveals that Rab12 interacts with the RILP homology domain (RHD) of one RILP monomer and a C-terminal threonine of the other RILP monomer in a homodimeric RILP complex; lysine-71 of Rab12 is critical for binding RILP-L1 and RILP-L2 but dispensable for RILP binding; mutational analyses of RILP RHD demonstrate its involvement in mast cell secretory granule transport regulation. Molecular dynamics simulations, functional mutagenesis, peptide inhibition assays, biochemical binding assays Scientific Reports Medium 33986343
2024 DENND6A acts as a GEF for Rab34 and as an effector of Arl8b; Arl8b recruits DENND6A to peripheral lysosomes, where it activates Rab34, which in turn recruits a RILP/dynein complex to drive lysosome retrograde transport and juxtanuclear repositioning; loss of DENND6A impairs autophagic flux. Cell-based GEF screen, co-immunoprecipitation, siRNA knockdown, lysosome positioning assay, autophagic flux assay Nature Communications High 38296963
2024 Rab7 phosphorylation at tyrosine 183 in diabetic cardiomyopathy promotes RILP recruitment to lipid droplets, enabling lysosomal degradation of lipid droplets via microlipophagy; Rab7 activator ML-098 enhances RILP levels and rescues cardiac dysfunction. RNA-seq, conditional knockout mice, phospho-specific analysis, in vivo pharmacological intervention, cardiac functional assays Advanced Science Medium 38837607
2024 RILP induces late endosome/lysosome clustering that reduces ER-endolysosome contact sites; RILP interacts with ORP1L to competitively inhibit VAP-ORP1L contact site formation, blocking cholesterol flow from endolysosomes to ER and triggering RILP-dependent autophagy. Co-immunoprecipitation, immunofluorescence microscopy, cholesterol assays, autophagy assays, overexpression Cells Medium 39195203
2024 pH neutralization of late endosomes increases V1G1 (ATP6V1G1) subunit assembly on endosomal membranes; V1G1 stabilizes GTP-bound Rab7 via RILP interaction, leading to Rab7 hyperactivation, disrupted tubulation, and impaired CI-M6PR recycling, defining a V-ATPase–RILP–Rab7 feedback axis for endosomal pH control. LLOMe and NH4Cl treatments, dominant-active Rab7 mutants, co-immunoprecipitation, live-cell imaging, mannose-6-phosphate receptor trafficking assay Journal of Cell Science Medium 38578235
2025 RILP functions as a RAB7A-dependent dynein adaptor for late endosome motility in neuronal dendrites, promotes endosome carrier formation, and is required for retrograde transport and clearance of degradative cargos from dendrites; importantly, RAB7A-RILP interaction is not required for lysosomal fusion or somatic degradation, but RAB7A/RILP-dependent late endosome transport is required for dendrite arborization. Separation-of-function RAB7A-L8A mutant (RILP-binding deficient) expressed in hippocampal neurons, live-cell imaging, cargo degradation assays, dendrite morphology analysis bioRxivpreprint Medium bio_10.1101_2025.09.03.673267

Source papers

Stage 0 corpus · 61 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 The Rab7 effector protein RILP controls lysosomal transport by inducing the recruitment of dynein-dynactin motors. Current biology : CB 644 11696325
2009 Cholesterol sensor ORP1L contacts the ER protein VAP to control Rab7-RILP-p150 Glued and late endosome positioning. The Journal of cell biology 560 19564404
2001 Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. The EMBO journal 436 11179213
2007 Activation of endosomal dynein motors by stepwise assembly of Rab7-RILP-p150Glued, ORP1L, and the receptor betalll spectrin. The Journal of cell biology 383 17283181
2003 Phagosomes fuse with late endosomes and/or lysosomes by extension of membrane protrusions along microtubules: role of Rab7 and RILP. Molecular and cellular biology 350 12944476
2013 Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. Journal of cell science 163 23729732
2004 Salmonella impairs RILP recruitment to Rab7 during maturation of invasion vacuoles. Molecular biology of the cell 130 15121880
2007 RILP is required for the proper morphology and function of late endosomes. Journal of cell science 97 17959629
2008 Huntingtin regulates RE1-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF) nuclear trafficking indirectly through a complex with REST/NRSF-interacting LIM domain protein (RILP) and dynactin p150 Glued. The Journal of biological chemistry 87 18922795
2016 Folliculin directs the formation of a Rab34-RILP complex to control the nutrient-dependent dynamic distribution of lysosomes. EMBO reports 79 27113757
2012 The Rab interacting lysosomal protein (RILP) homology domain functions as a novel effector domain for small GTPase Rab36: Rab36 regulates retrograde melanosome transport in melanocytes. The Journal of biological chemistry 75 22740695
2017 Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A. The Journal of biological chemistry 67 28465352
2007 Mycobacterium bovis BCG disrupts the interaction of Rab7 with RILP contributing to inhibition of phagosome maturation. Journal of leukocyte biology 67 18040083
2014 RILP regulates vacuolar ATPase through interaction with the V1G1 subunit. Journal of cell science 65 24762812
2020 The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance. Developmental cell 57 32275887
2012 Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes. Journal of cell science 53 22275436
2012 The Rilp-like proteins Rilpl1 and Rilpl2 regulate ciliary membrane content. Molecular biology of the cell 51 23264467
2006 RILP interacts with VPS22 and VPS36 of ESCRT-II and regulates their membrane recruitment. Biochemical and biophysical research communications 49 17010938
2003 A unique region of RILP distinguishes it from its related proteins in its regulation of lysosomal morphology and interaction with Rab7 and Rab34. Molecular biology of the cell 47 14668488
2006 Characterization of the REST/NRSF-interacting LIM domain protein (RILP): localization and interaction with REST/NRSF. Journal of neurochemistry 45 16417580
2006 RILP interacts with the VPS22 component of the ESCRT-II complex. Biochemical and biophysical research communications 43 16857164
2019 LRRK1 phosphorylation of Rab7 at S72 links trafficking of EGFR-containing endosomes to its effector RILP. Journal of cell science 41 31085713
2019 RILP Restricts Insulin Secretion Through Mediating Lysosomal Degradation of Proinsulin. Diabetes 38 31624142
2010 IGF-I stimulates Rab7-RILP interaction during neuronal autophagy. Neuroscience letters 37 20849920
2016 Hepatitis C virus promotes virion secretion through cleavage of the Rab7 adaptor protein RILP. Proceedings of the National Academy of Sciences of the United States of America 35 27791088
2016 Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex. Journal of immunology (Baltimore, Md. : 1950) 33 26740112
2018 A non-canonical GTPase interaction enables ORP1L-Rab7-RILP complex formation and late endosome positioning. The Journal of biological chemistry 28 30012887
2009 Thiazolidinediones induce Rab7-RILP-MAPK-dependent juxtanuclear lysosome aggregation and reduce tumor cell invasion. Traffic (Copenhagen, Denmark) 28 20015112
2014 A new V-ATPase regulatory mechanism mediated by the Rab interacting lysosomal protein (RILP). Communicative & integrative biology 27 26843904
2024 Targeting Rab7-Rilp Mediated Microlipophagy Alleviates Lipid Toxicity in Diabetic Cardiomyopathy. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 25 38837607
2023 RILP inhibits tumor progression in osteosarcoma via Grb10-mediated inhibition of the PI3K/AKT/mTOR pathway. Molecular medicine (Cambridge, Mass.) 21 37789274
2020 Cln1-mutations suppress Rab7-RILP interaction and impair autophagy contributing to neuropathology in a mouse model of infantile neuronal ceroid lipofuscinosis. Journal of inherited metabolic disease 18 32279353
2015 RILP suppresses invasion of breast cancer cells by modulating the activity of RalA through interaction with RalGDS. Cell death & disease 18 26469971
2009 Detection of activated Rab7 GTPase with an immobilized RILP probe. Methods in molecular biology (Clifton, N.J.) 18 19347311
2024 DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy. Nature communications 17 38296963
2021 Vps34 Inhibits Hepatocellular Carcinoma Invasion by Regulating Endosome-Lysosome Trafficking via Rab7-RILP and Rab11. Cancer research and treatment 15 33781048
2020 Methylation of RILP in lung cancer promotes tumor cell proliferation and invasion. Molecular and cellular biochemistry 14 33128214
2001 Expression analysis and chromosomal assignment of PRA1 and RILP genes. Biochemical and biophysical research communications 14 11520070
2020 A RILP-regulated pathway coordinating autophagosome biogenesis with transport. Autophagy 12 32597306
2018 Caspase-1 regulates cellular trafficking via cleavage of the Rab7 adaptor protein RILP. Biochemical and biophysical research communications 12 30100068
2005 Assay and functional properties of Rab34 interaction with RILP in lysosome morphogenesis. Methods in enzymology 12 16473629
2024 Collapse of late endosomal pH elicits a rapid Rab7 response via the V-ATPase and RILP. Journal of cell science 10 38578235
2017 Characterization of the role of RILP in cell migration. European journal of histochemistry : EJH 10 28735522
2007 Phagosome maturation in unicellular eukaryote Paramecium: the presence of RILP, Rab7 and LAMP-2 homologues. European journal of histochemistry : EJH 10 17921111
2005 Expression, assay, and functional properties of RILP. Methods in enzymology 9 16473628
2015 Quantitative bead-based flow cytometry for assaying Rab7 GTPase interaction with the Rab-interacting lysosomal protein (RILP) effector protein. Methods in molecular biology (Clifton, N.J.) 8 25800855
2023 Disruption of Golgi markers by two RILP-directed shRNAs in neurons: A new role for RILP or a neuron-specific off-target phenotype? The Journal of biological chemistry 5 37315786
2021 Roles of the multivalent dynein adaptors BicD2 and RILP in neurons. Neuroscience letters 5 33667600
2025 RILP cleavage links an inflammatory state to enhanced tau propagation in a cell culture model of Alzheimer's disease. Molecular biology of the cell 4 40137558
2024 USP5-dependent HDAC1 promotes cisplatin resistance and the malignant progression of non-small cell lung cancer by regulating RILP acetylation levels. Thoracic cancer 4 39582290
2023 A Novel Role for RILP in Regulating Osteoclastogenesis and Bone Resorption. Laboratory investigation; a journal of technical methods and pathology 4 36801641
2021 Biochemical and structural insights into Rab12 interactions with RILP and its family members. Scientific reports 4 33986343
2019 Molecular cloning of the Rab7 effector RILP (Rab-interacting lysosomal protein) in Litopenaeus vannamei and preliminary analysis of its role in white spot syndrome virus infection. Fish & shellfish immunology 4 31059814
2025 Mechanism of mTOR/RILP-regulated autophagic flux in increased susceptibility to myocardial ischemia-reperfusion in diabetic mice. Frontiers in pharmacology 3 39958873
2023 Evolutional insights into the interaction between Rab7 and RILP in lysosome motility. iScience 3 37534141
2022 RILP inhibits proliferation, migration, and invasion of PC3 prostate cancer cells. Acta histochemica 3 35981451
2024 RILP Induces Cholesterol Accumulation in Lysosomes by Inhibiting Endoplasmic Reticulum-Endolysosome Interactions. Cells 2 39195203
2024 Collapse of late endosomal pH elicits a rapid Rab7 response via V-ATPase and RILP. bioRxiv : the preprint server for biology 1 37961579
2011 RE1-silencing transcription factor (REST) and REST-interacting LIM domain protein (RILP) affect P19CL6 differentiation. Genes to cells : devoted to molecular & cellular mechanisms 1 21199191
2023 "Disruption of Golgi markers by two RILP-directed shRNAs in neurons: a new role for RILP or a neuron-specific off-target phenotype?". bioRxiv : the preprint server for biology 0 36945482
2023 Functional assessment of lysosomal Rab7 and RILP with RNA interference and overexpression in Spodoptera frugiperda Sf9 cell lines. STAR protocols 0 37851568