Affinage

RAB34

Ras-related protein Rab-34, isoform NARR · UniProt P0DI83

Length
198 aa
Mass
21.1 kDa
Annotated
2026-06-10
39 papers in source corpus 27 papers cited in narrative 27 extracted findings
Cross-family judge faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAB34 is a Golgi-associated small GTPase that coordinates several distinct membrane-trafficking programs through nucleotide-state-dependent engagement of effectors (PMID:12475955, PMID:16138900). In its lysosomal-positioning role, GTP-loaded RAB34 binds the shared effector RILP—an interaction requiring membrane prenylation and the switch I residue Lys82—to redistribute lysosomes to the peri-Golgi region and restrict their motility (PMID:12475955, PMID:16473629); this axis is set in motion by the GEF DENND6A, which is recruited to peripheral lysosomes by Arl8b to activate RAB34 and assemble a RILP/dynein complex driving retrograde transport that feeds into autophagic flux (PMID:38296963), while folliculin loads active RAB34 onto RILP at Golgi–lysosome contact sites to cluster lysosomes upon nutrient withdrawal (PMID:27113757). At the Golgi, RAB34 acts downstream of the ER to mediate intra-Golgi and Golgi-to-plasma-membrane secretory transport through its GTP-dependent effector Munc13-2/hmunc13 (via the MHD2 domain), a pathway coupling glucose stimulation to constitutive secretion (PMID:16138900, PMID:17881736, PMID:19641095). RAB34 also promotes macropinosome formation from membrane ruffles in a Rac1/WAVE2-dependent manner (PMID:12446704) and is exploited by intracellular pathogens for entry and vacuole maturation (PMID:18005733, PMID:28185347). A central, selective function is in ciliogenesis: RAB34 is an essential mediator of the intracellular (but not extracellular) ciliary pathway, localizing near the mother centriole and the ciliary sheath and driving ciliary vesicle formation, with both GTP binding/turnover and an atypical N-terminal LPQ motif (aa 16–18) rather than the canonical switch II region required for effector engagement (PMID:30301781, PMID:32669361, PMID:33989527, PMID:33989524, PMID:33860735). Loss of this activity impairs Hedgehog signaling and Gli3 repressor processing, and bi-allelic loss-of-function RAB34 variants cause an oral-facial-digital syndrome through defective intracellular ciliogenesis (PMID:30301781, PMID:37384395). RAB34 is additionally tyrosine-phosphorylated by Src at Y247 to regulate integrin β3 endocytosis/recycling and cell migration (PMID:29622794), and its abundance is controlled by proteasome-dependent degradation promoted by PSMB1, which dampens MEK/ERK signaling (PMID:38159835).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2002 High

    Established RAB34's first cellular activities—defining it simultaneously as a regulator of actin-driven macropinocytosis at the cell surface and of lysosome positioning at the Golgi—revealing an unusually broad trafficking remit for a single Rab.

    Evidence Localization, gain/loss-of-function, in vitro GTPase assay and Rac1/WAVE2 epistasis in fibroblasts; GFP-mutant imaging plus yeast two-hybrid/GST pulldown for RILP

    PMID:12446704 PMID:12475955

    Open questions at the time
    • The GAP acting on RAB34 in cells was not identified
    • How one Rab partitions between ruffle and Golgi pools was not resolved
  2. 2003 High

    Defined RILP as a shared effector of both Rab7 and RAB34 through a discrete 62-residue region, explaining how RAB34 engages the lysosomal-positioning machinery despite acting from the Golgi.

    Evidence Co-IP, GST pulldown and domain-swap chimeras with lysosome morphology readout

    PMID:14668488

    Open questions at the time
    • Structural basis of dual Rab recognition by RILP not determined
    • Whether Rab7 and RAB34 compete for RILP in vivo unaddressed
  3. 2005 High

    Confirmed RILP as a direct GTP-dependent RAB34 effector and identified hmunc13 as a separate Golgi effector, distinguishing RAB34's lysosomal-positioning and secretory effector arms.

    Evidence In vitro direct binding/GTP overlay with K82 mutagenesis (RILP); bacterial two-hybrid, co-IP and MHD2 deletion (hmunc13); RILP self-association by two-hybrid/co-IP

    PMID:15996637 PMID:16138900 PMID:16473629

    Open questions at the time
    • The downstream output of the RAB34–hmunc13 complex at the Golgi was not defined at this stage
    • DAG-dependent translocation kinetics not quantified
  4. 2007 High

    Placed RAB34 function downstream of the ER specifically at the Golgi, showing it is required for intra-Golgi and Golgi-to-plasma-membrane transport but not ER-to-medial-Golgi traffic, and that pathogens hijack its macropinocytic activity.

    Evidence RNAi/DN with VSVG-GFP and EndoH assays plus rescue; siRNA/DN with viral entry and occludin internalization readouts

    PMID:17881736 PMID:18005733

    Open questions at the time
    • The molecular step within the Golgi controlled by RAB34 not pinpointed
    • Link between secretory and macropinocytic roles unexplained
  5. 2009 Medium

    Connected the RAB34–munc13-2 secretory axis to physiological signaling, showing hyperglycemia upregulates munc13-2 to drive RAB34-dependent constitutive secretion.

    Evidence siRNA of both components, VSVG-GFP and fibronectin secretion assays, MHD2 deletion in mesangial cells

    PMID:19641095

    Open questions at the time
    • Whether RAB34 activation state changes with glucose not measured
    • Single-lab, two-cell-type scope
  6. 2012 High

    Extended RAB34's Munc13-2-dependent activity to phagolysosome biogenesis, showing it drives phagosome-lysosome fusion and microbial killing independently of Rab7.

    Evidence siRNA, active-RAB34 overexpression, EM, cargo-delivery and mycobacterial killing assays with Rab7 epistasis

    PMID:23197834

    Open questions at the time
    • How RAB34 selects cargo for size-selective transfer unknown
    • Relationship to RAB34's Golgi pool unclear
  7. 2013 Medium

    Dissociated RAB34's two lysosome-directed activities, showing lysosomal redistribution and phagolysosome fusion are functionally separable.

    Evidence Quantitative confocal organelle-positioning analysis in macrophages

    PMID:23871933

    Open questions at the time
    • Mechanistic basis for separating the two activities not defined
    • Single imaging method
  8. 2016 High

    Revealed that folliculin acts not as a GEF but as a factor that loads active RAB34 onto RILP at Golgi–lysosome contact sites, mechanistically explaining nutrient-regulated lysosome clustering.

    Evidence FLCN/RAB34 knockdown, live lysosome-motility imaging, in vitro reconstitution with purified FLCN-DENN, RAB34 and RILP, GEF assay

    PMID:27113757

    Open questions at the time
    • The bona fide GEF for RAB34 remained unknown
    • Structural detail of the loading reaction absent
  9. 2017 Medium

    Showed bacterial effectors target RAB34, with Salmonella SopD2 binding RAB34 to modulate vacuole maturation and intracellular growth.

    Evidence Binding assay, siRNA, SCV maturation and intracellular growth assays

    PMID:28185347

    Open questions at the time
    • Whether SopD2 alters RAB34 nucleotide state not shown
    • Direct vs indirect binding not resolved
  10. 2018 High

    Identified RAB34's defining in vivo role in ciliogenesis and a parallel role in integrin trafficking, linking it to Hedgehog signaling and to Src-driven cell migration.

    Evidence Rab34 knockout mice with ciliary vesicle/Gli3 analysis; co-IP, shRNA, Src kinase assay, Y247 mutants and migration/recycling assays

    PMID:29622794 PMID:30301781

    Open questions at the time
    • Effector linking RAB34 to ciliary vesicle fusion not identified
    • How Y247 phosphorylation alters RAB34 activity mechanistically unclear
  11. 2020 High

    Pinpointed an atypical effector-engagement mechanism for ciliogenesis, showing the unique N-terminal region (aa 1–49) rather than switch II is essential, distinguishing RAB34 from canonical Rabs.

    Evidence siRNA screen of 62 Rabs, CRISPR KO, domain deletion/point mutagenesis with cilia readout

    PMID:32669361

    Open questions at the time
    • The N-terminal-binding ciliogenesis effector not identified
  12. 2021 High

    Defined RAB34 as a selective mediator of the intracellular ciliogenesis pathway acting at ciliary vesicle formation near the mother centriole, requiring both GTP binding and turnover and a discrete N-terminal LPQ motif.

    Evidence KO in multiple cell lines, GTPase mutants, ciliary-sheath imaging, Ift27 BioID, and LPQ-to-AAA rescue assays

    PMID:33860735 PMID:33989524 PMID:33989527

    Open questions at the time
    • The downstream vesicle-fusion machinery engaged by RAB34 remains unidentified
    • Why extracellular ciliogenesis bypasses RAB34 not explained
  13. 2022 Medium

    Extended RAB34's lysosome/degradative functions to cell differentiation, showing it bidirectionally controls osteoclastogenesis via lysosomal receptor degradation and protease secretion.

    Evidence Gain/loss-of-function in osteoclast precursors with receptor degradation, transcription factor and protease secretion assays

    PMID:35285960

    Open questions at the time
    • Which effector mediates osteoclast receptor degradation not defined
    • Single-lab scope
  14. 2023 High

    Established disease causation and a degradation control point, linking bi-allelic RAB34 loss-of-function to oral-facial-digital syndrome and showing PSMB1 drives RAB34 proteasomal degradation to restrain MEK/ERK signaling.

    Evidence Exome sequencing with patient-variant rescue and cilia/centriole assays; co-IP, proteasome-inhibitor rescue, ERK assay and PDX model

    PMID:37384395 PMID:38159835

    Open questions at the time
    • How specific variants impair ciliogenesis while retaining centriole recruitment unresolved
    • Mechanism linking RAB34 levels to MEK/ERK not fully defined
  15. 2024 High

    Broadened RAB34's organellar repertoire to lipid droplets and reconstructed the activating GEF cascade, defining Arl8b→DENND6A→RAB34→RILP/dynein as a linear axis upstream of retrograde lysosomal transport and autophagy.

    Evidence Cell-based GEF screen, DENND6A KO, RAB34 activation/co-IP, lysosome positioning and autophagic flux assays; adipocyte imaging with RAB34–UBA1–FABP5 co-IP and ubiquitination/adiponectin assays; craniofacial conditional KO with cilia and collagen trafficking assays

    PMID:38183057 PMID:38296963 PMID:38852507

    Open questions at the time
    • How DENND6A is selectively delivered to the RAB34 pool versus other targets unclear
    • Coordination of RAB34's Golgi, lipid-droplet and ciliary pools not integrated
  16. 2026 Medium

    Linked RAB34 to regulated secretion in endocrine cells, showing it directs insulin secretory vesicles toward autophagic degradation and is repressed by miR-9.

    Evidence Overexpression/siRNA with EM, vesicle imaging and dual-luciferase miR-9 target validation in β cells

    PMID:41891636

    Open questions at the time
    • Whether this reflects RAB34's known autophagy or secretory effectors not tested
    • Single-lab finding

Open questions

Synthesis pass · forward-looking unresolved questions
  • The ciliogenesis effector engaged by RAB34's atypical N-terminal LPQ motif, and how RAB34's many parallel functions (Golgi secretion, macropinocytosis, lysosome positioning, ciliary vesicle formation, lipid droplets) are partitioned within a single cell, remain unresolved.
  • No effector identified for the N-terminal ciliogenesis mechanism
  • No unifying model for how RAB34 is allocated across organelles
  • The endogenous GAP inferred in 2002 remains unidentified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Localization
GO:0005794 Golgi apparatus 4 GO:0005764 lysosome 2 GO:0005783 endoplasmic reticulum 2 GO:0005815 microtubule organizing center 2 GO:0005886 plasma membrane 2 GO:0005929 cilium 2 GO:0005811 lipid droplet 1
Pathway
R-HSA-1852241 Organelle biogenesis and maintenance 4 R-HSA-162582 Signal Transduction 3 R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-9609507 Protein localization 3 R-HSA-9612973 Autophagy 2 R-HSA-1643685 Disease 1
Partners
DENND6AFLCNITGB3MUNC13-2PSMB1RILPSRCUBA1

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 Rab34 (Rah) is associated with membrane ruffles and nascent macropinosomes in fibroblasts. Overexpression of Rab34 increases macropinosome number ~2-fold, and dominant-negative Rab34 suppresses macropinosome formation induced by PDGF or phorbol ester. Rab34-promoted macropinosome formation requires Rac1 and WAVE2. Wild-type Rab34 has extremely low GTPase activity in vitro but appreciable activity in vivo, suggesting a specific GAP acts on it in cells. Fluorescence microscopy (colocalization with actin/macropinosome markers), overexpression and dominant-negative transfection, in vitro GTPase assay, dominant-negative Rac1/WAVE2 epistasis The Journal of biological chemistry High 12446704
2002 Rab34 is associated primarily with the Golgi apparatus and, when expressed in GTP-restricted (active) form, causes redistribution of lysosomes from the cell periphery to the peri-Golgi region. This activity requires membrane association via prenylation and direct interaction with RILP (Rab-interacting lysosomal protein). Lys82 in the switch I region of Rab34 is essential for RILP interaction and lysosomal repositioning. GFP-tagged Rab34 overexpression (WT, GTP-restricted, GDP-restricted mutants), lysosome marker imaging, prenylation-deficient mutant analysis, yeast two-hybrid and GST pulldown for RILP interaction, site-directed mutagenesis (K82) Molecular biology of the cell High 12475955
2003 RILP interacts selectively with GTP-bound forms of both Rab7 and Rab34. A unique 62-residue region (aa 272–333) within RILP is necessary for interaction with both GTPases and for regulating lysosomal morphology; transferring this region into the related but inactive RLP1 confers lysosomal regulation. Co-immunoprecipitation, GST pulldown, chimeric protein analysis, overexpression with lysosome morphology readout Molecular biology of the cell High 14668488
2005 RILP is a direct effector of Rab34; GST pulldown and direct binding assays in vitro confirm RILP binds preferentially to GTP-loaded Rab34, with K82 being a key contact residue. Expression of active Rab34 redistributes lysosomes to peri-Golgi in a K82-dependent manner, confirming the RILP interaction underlies lysosomal repositioning. GTP overlay assay, GST pulldown, in vitro direct binding assay, site-directed mutagenesis (K82), fluorescence microscopy Methods in enzymology High 16473629
2005 Hmunc13 (a DAG-binding cytosolic protein) is an effector of Rab34 at the Golgi. Hmunc13 binds specifically to GTP-bound Rab34 via its MHD2 domain. DAG activation translocates hmunc13 to the Golgi where it engages active Rab34; deletion of MHD2 abolishes the interaction. Bacterial two-hybrid screen, co-immunoprecipitation, GST-fusion pulldown with GTP/GDP-loaded Rab34 mutants, radioactive GTP overlay assay, deletion mutagenesis of MHD2 Traffic (Copenhagen, Denmark) High 16138900
2005 RILP is capable of self-interaction (homo-dimerization), demonstrated first by yeast two-hybrid and confirmed by co-immunoprecipitation in HeLa cells. This property is shared with other Rab effectors and is relevant to its function as a shared effector of Rab7 and Rab34. Yeast two-hybrid, co-immunoprecipitation Biochemical and biophysical research communications Medium 15996637
2007 Rab34 is required for intra-Golgi transport but not for ER-to-medial Golgi traffic. Depletion of Rab34 by dominant-negative expression or RNAi blocks transport of VSVG-GFP from Golgi to plasma membrane; rescue with mouse Rab34 confirms specificity. EndoH resistance assays show ER-to-medial Golgi transit is intact, placing Rab34's function downstream of the ER at the Golgi. Dominant-negative transfection, siRNA knockdown, VSVG-GFP secretory transport assay, endoglycosidase H resistance assay, immunoelectron microscopy, immunocytochemistry Molecular biology of the cell High 17881736
2007 CVB (coxsackievirus B) entry into polarized epithelial cells via macropinocytosis requires Rab34, Ras, and Rab5 activity. Rab34 activity is necessary for occludin internalization within macropinosomes and for viral entry into the cytoplasm; Rab34-dependent macropinocytosis also requires caveolin but not dynamin. siRNA knockdown of Rab34, dominant-negative constructs, pharmacological inhibitors of macropinocytosis, viral infection assay, fluorescence microscopy Cell host & microbe High 18005733
2009 Rab34 and munc13-2 constitute a pathway coupling hyperglycemia to stimulated constitutive protein secretion. High glucose upregulates munc13-2, which increases VSVG-GFP secretion; siRNA knockdown of either munc13-2 or rab34 abolishes this. The interaction requires the MHD2 domain of munc13-2, and high glucose-stimulated fibronectin secretion in mesangial cells is similarly abolished by munc13-2 knockdown. siRNA knockdown of rab34 and munc13-2, VSVG-GFP secretion assay, MHD2 deletion mutant, fibronectin secretion assay in rat mesangial cells American journal of physiology. Cell physiology Medium 19641095
2010 Rab36, a paralog of Rab34 with 56% identity, also localizes to the Golgi and regulates late endosome/lysosome positioning through interaction with RILP (C-terminal aa199–401), mirroring the Rab34 mechanism. This finding demonstrates the Rab34-RILP lysosome-positioning mechanism is shared with Rab36. EGFP-Rab36 localization, GTP/GDP mutant analysis, lysosome marker imaging, GST pulldown for RILP binding Molecular membrane biology Medium 19961360
2012 Rab34 controls phagolysosome biogenesis via recruitment of Munc13-2. Rab34 knockdown impairs phagosome-lysosome fusion; active Rab34 promotes it in a Rab7-independent manner, mediating size-selective transfer of late endosomal/lysosomal cargo into phagosomes. Loss of Rab34 results in mycobacterial survival, while Rab34 expression promotes mycobacterial killing. siRNA knockdown, overexpression of active Rab34, electron microscopy for phagolysosome biogenesis, cargo delivery assay, mycobacterial killing assay, epistasis with Rab7 Proceedings of the National Academy of Sciences of the United States of America High 23197834
2013 While Rab34 regulates both lysosomal positioning and lysosome-to-phagosome fusion, Rab34-dependent redistribution of lysosomes does not significantly alter the spatial distribution of phagolysosomes in macrophages, indicating these two Rab34-dependent processes are functionally separable. Confocal microscopy with 2D organelle positioning analysis, active Rab34 overexpression and pH manipulation in macrophages The international journal of biochemistry & cell biology Medium 23871933
2016 Folliculin (FLCN), the BHD syndrome tumor suppressor, promotes peri-nuclear lysosome clustering in response to serum/amino acid withdrawal and works through Rab34. Using purified recombinant proteins, the FLCN DENN domain does not act as a GEF for Rab34 but instead loads active Rab34 onto RILP, facilitating formation of a Rab34/RILP complex at contact sites between Golgi-proximal membranes and lysosomes that restricts lysosome motility. FLCN knockdown, Rab34 knockdown, live imaging of lysosome motility, in vitro reconstitution with purified recombinant proteins (FLCN-DENN, Rab34, RILP), GEF activity assay EMBO reports High 27113757
2017 The Salmonella effector SopD2 binds Rab34 and modulates its function. Depletion of Rab34 delays maturation of the Salmonella-containing vacuole (SCV) and inhibits intracellular S. typhimurium growth. SopD2-deficient bacteria are severely impaired in Rab34-depleted cells, indicating a compounding virulence effect. Co-immunoprecipitation/binding assay, siRNA knockdown of Rab34, intracellular bacterial growth assay, SCV maturation assay Cell biology international Medium 28185347
2018 Rab34 is required for early ciliary vesicle formation and ciliogenesis in vivo. Rab34 localizes to cilia, and Rab34 mutant mice exhibit ciliopathy phenotypes (polydactyly, cleft lip and palate). Mechanistically, Rab34 is required for the successive fusion of preciliary vesicles to generate ciliary vesicles and for migration of the mother centriole to the plasma membrane. Nonciliated Rab34-mutant cells fail to respond to Hedgehog signaling, and Gli3 processing to its truncated repressor form is reduced. Rab34 knockout mice, ciliogenesis assay in cultured cells and in vivo, immunofluorescence for ciliary vesicle intermediates, Hedgehog signaling assay (Gli3 processing by western blot) Journal of cell science High 30301781
2018 Rab34 binds to the cytoplasmic tail of integrin β3, and Rab34 depletion promotes integrin β3 degradation. EGF induces Rab34 translocation to membrane ruffles; Src kinase enhances this. Rab34 is tyrosine phosphorylated by Src at Y247, and a phosphomimetic mutant (Y247D) promotes cell migration, invasion, adhesion, and integrin β3 endocytosis and recycling. Co-immunoprecipitation (Rab34–integrin β3), shRNA knockdown, Src kinase phosphorylation assay, phosphomimetic/phosphonull mutants, cell migration/invasion/adhesion assays, integrin recycling assay Oncogene High 29622794
2020 Comprehensive siRNA screen identifies Rab34 as essential for serum starvation-induced ciliogenesis in hTERT-RPE1 cells. Rab34 KO drastically impairs ciliogenesis. Deletion/mutation analysis reveals that a unique long N-terminal region (aa 1–49) rather than the switch II region is essential, indicating Rab34 uses an atypical mechanism for ciliogenesis effector engagement. siRNA screen of 62 Rab GTPases, CRISPR/Cas9 knockout, deletion and point mutagenesis of Rab34 domains, cilia formation assay The Journal of biological chemistry High 32669361
2021 Rab34 is a selective mediator of intracellular (but not extracellular) ciliogenesis. Rab34 marks the ciliary sheath, a unique sub-domain of assembling intracellular cilia, and is required for ciliary vesicle formation at the mother centriole. Ciliogenesis requires both GTP binding and turnover by Rab34 (modulated by divergent residues in the GTPase domain). MDCK cells (which use the extracellular pathway) ciliate independently of Rab34 and its paralog Rab36. Rab34 KO in multiple cell lines (RPE1, NIH3T3, MDCK), GTPase-dead and constitutively active mutants, live imaging, immunofluorescence for pathway-specific intermediates Current biology : CB High 33989527
2021 Rab34 localizes to centrosomes near the mother centriole and is required for cilia formation in fibroblasts, blocking ciliogenesis at the ciliary vesicle formation step. In IMCD3 epithelial cells, Rab34 is required specifically for the internal (intracellular) ciliogenesis pathway but not for the external pathway. Rab34 was identified via proximity biotinylation using Ift27 as bait. Proximity biotinylation (BioID with Ift27 bait), Rab34 KO in fibroblasts and IMCD3 cells, immunofluorescence for ciliogenesis intermediates Current biology : CB High 33989524
2021 The N-terminal LPQ sequence (aa 16–18) of Rab34 is required for serum starvation-induced ciliogenesis in hTERT-RPE1 cells. Rab34 mutants lacking the N-terminal 18 aa or carrying LPQ-to-AAA mutations fail to rescue a Rab34-KO ciliogenesis defect. Deletion and alanine substitution mutagenesis, Rab34-KO rescue assay, cilia formation readout Small GTPases Medium 33860735
2022 Rab34 bidirectionally regulates osteoclastogenesis. As a negative regulator, Rab34 promotes lysosomal proteolysis of the osteoclastogenic surface receptors c-fms and RANK via the early endosome–late endosome–lysosome axis, reducing c-fos and NFATc1 transcriptional activity and attenuating osteoclast differentiation. Rab34 also modulates secretion of lysosomal proteases (MMP9, Cathepsin K) across osteoclast ruffled borders. Rab34 overexpression and knockdown in osteoclast precursor cells (RAW-D and bone marrow-derived macrophages), receptor degradation assay, transcription factor activity assay, protease secretion assay Cell biochemistry and function Medium 35285960
2023 PSMB1 (proteasome subunit beta type-1) directly binds RAB34 and promotes its proteasome-dependent degradation. This leads to inactivation of MEK/ERK signaling. Kinetin enhances the PSMB1–RAB34 interaction, facilitating RAB34 degradation and suppressing CRC progression. Co-immunoprecipitation (PSMB1–RAB34), proteasome inhibitor rescue assay, MEK/ERK phosphorylation assay, patient-derived xenograft model, drug screening by CADD Cancer letters Medium 38159835
2023 Pathogenic bi-allelic missense variants in RAB34 (clustered near the C-terminus) cause a novel oral-facial-digital syndrome (OFDS-RAB34). Protein products of these variants show strong loss of function: although some retain mother centriole recruitment, cells expressing mutant RAB34 have a significant defect in cilium assembly, establishing RAB34 as the first small GTPase linked to OFDS via impairment of intracellular ciliogenesis. Exome sequencing, expression of patient-derived variants in Rab34-KO cells, cilia assembly assay, immunofluorescence for centriole recruitment Human molecular genetics High 37384395
2024 DENND6A is a GEF that activates Rab34. Arl8b, a major GTPase on lysosomes, recruits DENND6A to peripheral lysosomes where it activates Rab34. Active Rab34 then recruits a RILP/dynein complex to lysosomes, promoting retrograde lysosomal transport. Loss of DENND6A impairs autophagic flux, placing the Arl8b→DENND6A→Rab34→RILP/dynein axis upstream of autophagy. Cell-based GEF screen, DENND6A knockdown/knockout, Rab34 activation assay, co-immunoprecipitation, lysosome positioning assay, autophagic flux assay Nature communications High 38296963
2024 In adipocytes, Rab34 translocates from the Golgi to ER-related compartments and then to the surface of lipid droplets during lipid droplet biogenesis. At the Golgi, Rab34 regulates cisternae integrity and adiponectin trafficking and oligomerization. At lipid droplets, Rab34 controls lipid accumulation and lipolysis through interaction with E1-ubiquitin ligase UBA1, which induces ubiquitination and proteasomal degradation of FABP5. Fluorescence imaging with organelle markers, Rab34 siRNA, co-immunoprecipitation (Rab34–UBA1–FABP5), ubiquitination assay, adiponectin secretion and oligomerization assay Journal of biomedical science Medium 38183057
2024 Rab34 is required for cilia-mediated Hedgehog signaling and osteogenic proliferation/differentiation in craniofacial development, and also regulates type I collagen trafficking from the ER to the Golgi independently of cilia. These demonstrate both ciliary and non-ciliary functions of Rab34 in osteogenesis. Rab34 conditional knockout in mouse craniofacial tissue, immunofluorescence for cilia, Hedgehog signaling assay, collagen trafficking assay Biochemical and biophysical research communications Medium 38852507
2026 Rab34 overexpression in pancreatic β cells reduces the number of proinsulin-containing particles, promotes proinsulin degradation, and directs insulin secretory vesicles toward the autophagic degradation pathway, resulting in decreased insulin secretion. Rab34 depletion maintains proinsulin and increases insulin secretion. miR-9 targets Rab34 to inhibit insulin secretion. Rab34 overexpression and depletion (siRNA), transmission electron microscopy for insulin granules, immunofluorescence for vesicle trafficking, dual-luciferase reporter for miR-9 target validation The Kaohsiung journal of medical sciences Medium 41891636

Source papers

Stage 0 corpus · 39 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5. Cell host & microbe 193 18005733
2002 Interorganellar regulation of lysosome positioning by the Golgi apparatus through Rab34 interaction with Rab-interacting lysosomal protein. Molecular biology of the cell 115 12475955
2002 Small GTPase Rah/Rab34 is associated with membrane ruffles and macropinosomes and promotes macropinosome formation. The Journal of biological chemistry 92 12446704
2016 Folliculin directs the formation of a Rab34-RILP complex to control the nutrient-dependent dynamic distribution of lysosomes. EMBO reports 79 27113757
2012 Size-dependent mechanism of cargo sorting during lysosome-phagosome fusion is controlled by Rab34. Proceedings of the National Academy of Sciences of the United States of America 57 23197834
2001 Maximal expression of membrane-bound nitrate reductase in Paracoccus is induced by nitrate via a third FNR-like regulator named NarR. Journal of bacteriology 53 11371524
2021 Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway. Current biology : CB 50 33989527
2007 Golgi-bound Rab34 is a novel member of the secretory pathway. Molecular biology of the cell 48 17881736
2003 A unique region of RILP distinguishes it from its related proteins in its regulation of lysosomal morphology and interaction with Rab7 and Rab34. Molecular biology of the cell 47 14668488
2010 Rab36 regulates the spatial distribution of late endosomes and lysosomes through a similar mechanism to Rab34. Molecular membrane biology 46 19961360
2018 Rab34 regulates adhesion, migration, and invasion of breast cancer cells. Oncogene 37 29622794
2004 Granzyme B encoded by the commonly occurring human RAH allele retains pro-apoptotic activity. The Journal of biological chemistry 35 14752093
2021 Rab34 is necessary for early stages of intracellular ciliogenesis. Current biology : CB 31 33989524
2005 The Rab-interacting lysosomal protein, a Rab7 and Rab34 effector, is capable of self-interaction. Biochemical and biophysical research communications 31 15996637
2018 Rab34 small GTPase is required for Hedgehog signaling and an early step of ciliary vesicle formation in mouse. Journal of cell science 27 30301781
2020 A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis. The Journal of biological chemistry 24 32669361
2005 Diacylglycerol-activated Hmunc13 serves as an effector of the GTPase Rab34. Traffic (Copenhagen, Denmark) 21 16138900
2024 DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy. Nature communications 17 38296963
2020 PVT1 Promotes the Proliferation and Migration of Non-Small Cell Lung Cancer via Regulating miR-148/RAB34 Signal Axis. OncoTargets and therapy 16 32184617
2023 Pathogenic RAB34 variants impair primary cilium assembly and cause a novel oral-facial-digital syndrome. Human molecular genetics 13 37384395
2017 Salmonella effector SopD2 interferes with Rab34 function. Cell biology international 13 28185347
2017 QPY/RAH haplotypes of the GZMB gene are associated with natural killer cell cytotoxicity. Immunogenetics 12 28653095
2005 Assay and functional properties of Rab34 interaction with RILP in lysosome morphogenesis. Methods in enzymology 12 16473629
2009 Rab34 and its effector munc13-2 constitute a new pathway modulating protein secretion in the cellular response to hyperglycemia. American journal of physiology. Cell physiology 11 19641095
2013 Spatial distribution of phagolysosomes is independent of the regulation of lysosome position by Rab34. The international journal of biochemistry & cell biology 8 23871933
2023 Discovery of Kinetin in inhibiting colorectal cancer progression via enhancing PSMB1-mediated RAB34 degradation. Cancer letters 7 38159835
2022 Rab34 plays a critical role as a bidirectional regulator of osteoclastogenesis. Cell biochemistry and function 6 35285960
2018 CSN6 and Rab34 Are Involved in Androgen Receptor Trafficking in Mouse Testicular Sertoli Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 6 29991022
2011 Identification and characterization of a novel ubiquitous nucleolar protein 'NARR' encoded by a gene overlapping the rab34 oncogene. Nucleic acids research 5 21586586
2005 Assays for functional properties of Rab34 in macropinosome formation. Methods in enzymology 5 16473590
2023 Compound heterozygous variants in RAB34 in a rare skeletal ciliopathy syndrome. Clinical genetics 4 37619988
2024 Localization, traffic and function of Rab34 in adipocyte lipid and endocrine functions. Journal of biomedical science 3 38183057
2024 Ciliary and non-ciliary functions of Rab34 during craniofacial bone development. Biochemical and biophysical research communications 3 38852507
2023 E2F1‑mediated RAB34 upregulation accelerates the proliferation and inhibits the cell cycle arrest and apoptosis of acute myeloid leukemia cells. Experimental and therapeutic medicine 3 37456160
2021 The N-terminal Leu-Pro-Gln sequence of Rab34 is required for ciliogenesis in hTERT-RPE1 cells. Small GTPases 3 33860735
2009 Rah, rah, ROS: metabolic changes caused by loss of adhesion induce cell death. Breast cancer research : BCR 3 19930622
2026 miR-9 Restricts Insulin Secretion by Targeting Rab34, Which Mediates Lysosomal Degradation of Proinsulin. The Kaohsiung journal of medical sciences 0 41891636
2023 PVT1 Promotes the Proliferation and Migration of Non-Small Cell Lung Cancer via Regulating miR-148/RAB34 Signal Axis [Retraction]. OncoTargets and therapy 0 37551310
2012 Distribution of QPY and RAH haplotypes of granzyme B gene in distinct Brazilian populations. Revista da Sociedade Brasileira de Medicina Tropical 0 22767096

Missed literature

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