Affinage

PRDX1

Peroxiredoxin-1 · UniProt Q06830

Length
199 aa
Mass
22.1 kDa
Annotated
2026-06-10
100 papers in source corpus 31 papers cited in narrative 31 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PRDX1 is a 2-Cys peroxiredoxin that provides essential antioxidant defence, with its catalytic peroxidatic Cys52 reducing H2O2 through a fast disulfide-relay cycle that makes it relatively resistant to hyperoxidation (PMID:12891360, PMID:30284335, PMID:37562742). Genetic loss causes erythrocyte oxidative damage and haemolytic anaemia, impaired fibroblast proliferation, and increased sensitivity to oxidative DNA damage, while the Cys52Ser peroxidase-dead knock-in reduces NASH and fibrosis by suppressing NF-κB and STAT1 signalling, demonstrating that catalytic activity drives both protective and pro-inflammatory phenotypes (PMID:12891360, PMID:37562742). Beyond bulk ROS scavenging, PRDX1 acts as a redox relay and signalling adaptor: it reductively activates GDE2 to permit motor neuron differentiation (PMID:19766572), engages MAPK pathway components by differentially protecting MKP-1 versus MKP-5 in an H2O2-dose-dependent manner (PMID:23334324), binds ASK1 at elevated H2O2 (PMID:34209102), and inhibits TRAF6 ubiquitin-ligase activity to restrain NF-κB activation and autophagy (PMID:29929436). PRDX1 also functions as a molecular chaperone for the NRF2 axis, binding CUL3 via Cys83 to block NRF2 ubiquitination and induce GPX4 transcription, thereby suppressing ferroptosis (PMID:39430237). It translocates to the nucleus during the DNA damage response to scavenge nuclear ROS and sustain aspartate-dependent nucleotide synthesis (PMID:37259925), is enriched in telomeric chromatin where it cooperates with MTH1 to limit oxidative guanine damage and telomere shortening (PMID:29773556, PMID:33147465), and promotes CUL5 neddylation through a PRDX1-oligomer/UBE2F/CUL5 tricomplex to degrade pro-apoptotic NOXA (PMID:33712558). PRDX1 activity is tuned by multiple post-translational modifications, including MOF-mediated K197 acetylation that prevents hyperoxidation, Pin1/PP2A-mediated dephosphorylation of Thr90 that restores peroxidase activity, and ZNF207-driven K67 lactylation that promotes nuclear translocation and NRF2 activation (PMID:39207899, PMID:23421996, PMID:40680452). Its active-site cysteine is targeted by several covalent small-molecule inhibitors whose binding mode has been resolved by co-crystallography, providing a structural basis for selective inhibition (PMID:40645965, PMID:40546088).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 2003 High

    Established that PRDX1 is physiologically essential for antioxidant defence rather than redundant, by showing knockout animals suffer systemic oxidative pathology.

    Evidence Targeted knockout mouse with erythrocyte ROS, haemoglobin stability, Heinz body, and NK/fibroblast assays

    PMID:12891360

    Open questions at the time
    • Did not resolve which catalytic residue or relay mechanism underlies protection
    • Did not distinguish cell-autonomous from systemic effects
  2. 2009 High

    Moved PRDX1 beyond bulk scavenging by demonstrating it acts as a specific redox enzyme that reductively activates a defined substrate to control a developmental program.

    Evidence Co-IP, thiol-dead PRDX1 and disulfide-null GDE2 mutants, motor neuron differentiation in chick spinal cord

    PMID:19766572

    Open questions at the time
    • Whether other transmembrane substrates are activated this way unknown
    • Cytosolic-to-membrane targeting mechanism not defined
  3. 2013 Medium

    Showed PRDX1's oxidation state itself encodes signalling information, partitioning MAPK phosphatase protection in an H2O2-dose-dependent manner.

    Evidence Reciprocal Co-IP, Cys52 mutants, H2O2 dose-response, p38 activity in breast epithelial cells

    PMID:23334324

    Open questions at the time
    • Direct redox transfer between PRDX1 and MKPs not reconstituted
    • Single cell-type context
  4. 2013 Medium

    Identified phosphorylation as a reversible off-switch for PRDX1, with Pin1/PP2A restoring peroxidase activity, linking proline isomerisation to redox capacity.

    Evidence Proteomics, Co-IP, Thr90 mutagenesis, Pin1 KO/rescue MEFs, peroxidase assay

    PMID:23421996

    Open questions at the time
    • Kinase phosphorylating Thr90 not identified here
    • In vivo relevance not tested
  5. 2018 Medium

    Defined PRDX1 as a non-catalytic inhibitor of TRAF6 ubiquitin-ligase activity, coupling it to NF-κB inflammation and autophagy control.

    Evidence Co-IP to TRAF6 RING domain, ECSIT/BECN1 ubiquitination, knockdown across three cancer lines

    PMID:29929436

    Open questions at the time
    • Whether this requires PRDX1 redox cycling unclear
    • Stoichiometry of inhibition not quantified
  6. 2018 High

    Provided the kinetic basis for PRDX1's distinct biological behaviour, measuring fast resolution that disfavours hyperoxidation relative to PRDX2.

    Evidence In vitro kinetics with recombinant PRDX1/PRDX2, intrinsic fluorescence

    PMID:30284335

    Open questions at the time
    • In-cell relevance of measured rates not directly validated
  7. 2018 High

    Placed PRDX1 at telomeric chromatin protecting genome integrity by cooperating with MTH1 against oxidised guanine.

    Evidence CRISPR double knockout, telomere length, telomerase activity, ROS quantification

    PMID:29773556

    Open questions at the time
    • How PRDX1 is recruited to telomeres not established
  8. 2018 Medium

    Showed PRDX1 can act as a positive signalling cofactor, heterodimerising with p38α to amplify MET/HGF-driven invasion.

    Evidence Co-IP, migration assays, glioma mouse survival and brain imaging

    PMID:29582423

    Open questions at the time
    • Redox dependence of the heterodimer not dissected
    • Direct vs indirect p38 stabilisation unresolved
  9. 2020 Medium

    Refined the telomere role by showing PRDX1 supports PARP-dependent telomeric repair, preventing single- to double-strand break conversion.

    Evidence PRDX1 depletion, telomeric ChIP, PARP inhibition, DNA damage markers

    PMID:33147465

    Open questions at the time
    • Direct enzymatic role at break sites versus general ROS control not separated
  10. 2021 Medium

    Demonstrated a scaffold-independent PRDX1:ASK1 redox relay at high H2O2, distinguishing PRDX1 relay logic from PRDX2.

    Evidence Cellular and in vitro interaction assays, H2O2 dose-response, PRDX2 comparison

    PMID:34209102

    Open questions at the time
    • Downstream ASK1 signalling output not quantified
    • Disulfide intermediate not mapped
  11. 2021 Medium

    Revealed an oligomer-specific scaffolding function whereby PRDX1 enables CUL5 neddylation to degrade pro-apoptotic NOXA.

    Evidence Co-IP tricomplex, knockdown, oligomerisation inhibition, NOXA stability and neddylation assays

    PMID:33712558

    Open questions at the time
    • Structural basis of UBE2F/CUL5 docking on PRDX1 oligomer unknown
  12. 2023 Medium

    Established a nuclear DNA-damage-response role for PRDX1 linking nuclear ROS scavenging to aspartate availability and nucleotide synthesis.

    Evidence Functional genomics, chromatin proteomics, metabolomics, PRDX1 KO, fractionation

    PMID:37259925

    Open questions at the time
    • Mechanism of nuclear import signal not defined
    • Direct vs indirect aspartate link unresolved
  13. 2023 High

    Genetically proved Cys52 peroxidase activity is required for PRDX1's pro-inflammatory function in vivo via NF-κB/STAT1.

    Evidence Cys52Ser knock-in mice, coupled peroxidase assay, NASH diet models, RNA-seq

    PMID:37562742

    Open questions at the time
    • Catalytic-independent signalling functions not addressed by this allele
  14. 2024 High

    Identified MOF-mediated K197 acetylation as a writer-controlled switch protecting PRDX1 from hyperoxidation, coupling acetylation loss to inflammatory glycolytic reprogramming.

    Evidence MOF as acetyltransferase, K197 MS, MOF KO macrophages, LPS, ERK/glycolysis readouts

    PMID:39207899

    Open questions at the time
    • Deacetylase removing K197 not identified
    • Structural effect of K197 acetylation on active site unresolved
  15. 2024 High

    Showed PRDX1 functions as a chaperone for the NRF2-GPX4 anti-ferroptosis axis through Cys83-dependent CUL3 binding.

    Evidence IP-MS, Co-IP, Cys83Ser mutant, NRF2 ubiquitination, PRDX1 KO CRC mice, RNA-seq

    PMID:39430237

    Open questions at the time
    • Whether chaperone activity is redox-state dependent unclear
  16. 2024 Medium

    Defined PRDX1 protein stability and activity as controlled by interacting partners that tune disulfide cycling and degradation (HJURP redox cycling, IRAK1/HECTD3 turnover, Hspb1/Anxa2 Thr90 phosphorylation, Cofilin actin turnover, DOK3 degradation).

    Evidence Co-IP, cysteine and Thr90 mutagenesis, ubiquitination and autophagy-lysosome assays, in vivo disease models

    PMID:37031183 PMID:38481805 PMID:38738971 PMID:39405980 PMID:40893682

    Open questions at the time
    • Most interactions rest on single-lab Co-IP without reciprocal structural validation
    • Interplay among these regulators not integrated
  17. 2025 Medium

    Showed lactylation at K67 by ZNF207 and transcriptional repression by BACH1 add further layers controlling PRDX1 nuclear localisation and abundance in disease contexts.

    Evidence CRISPR screen, K67 lactylation mapping, nuclear fractionation, ChIP/luciferase, drug-resistance and ischemia models

    PMID:39025461 PMID:40680452

    Open questions at the time
    • Eraser of K67 lactylation unknown
    • Crosstalk between transcriptional and PTM control not addressed
  18. 2025 High

    Validated PRDX1's active-site cysteine as a druggable covalent target and provided a co-crystal structure enabling structure-based inhibitor design.

    Evidence ABPP/pull-down, CETSA, SPR/fluorescence titration, co-crystal structure with celastrol derivative, IC50, ferroptosis xenografts (18β-GA, artesunate, LC-PDin20)

    PMID:36105163 PMID:40546088 PMID:40645965

    Open questions at the time
    • Selectivity over PRDX2 for several compounds incomplete
    • In vivo therapeutic window not established

Open questions

Synthesis pass · forward-looking unresolved questions
  • How PRDX1's many context-specific roles (cytosolic scavenging, nuclear DNA-damage response, signalling relays, scaffolding/chaperone functions) are coordinated by oligomeric state and the PTM code remains unresolved.
  • No unified model linking redox state, oligomerisation, and partner selection
  • Quantitative contribution of each function in normal physiology unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016209 antioxidant activity 3 GO:0016491 oxidoreductase activity 3 GO:0098772 molecular function regulator activity 3 GO:0044183 protein folding chaperone 1 GO:0140096 catalytic activity, acting on a protein 1
Localization
GO:0005829 cytosol 3 GO:0000228 nuclear chromosome 2 GO:0005634 nucleus 2
Pathway
R-HSA-162582 Signal Transduction 4 R-HSA-5357801 Programmed Cell Death 3 R-HSA-73894 DNA Repair 3 R-HSA-8953897 Cellular responses to stimuli 3 R-HSA-9612973 Autophagy 3
Partners
ASK1CFL1CUL3CUL5GDE2MAPK14MTH1TRAF6
Complex memberships
PRDX1-oligomer/UBE2F/CUL5 neddylation tricomplex

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 Prdx1 knockout mice develop severe haemolytic anaemia with increased erythrocyte ROS, protein oxidation, haemoglobin instability, and Heinz body formation, demonstrating Prdx1's essential role as an antioxidant defence in erythrocytes. Prdx1-deficient fibroblasts show decreased proliferation and increased sensitivity to oxidative DNA damage, and Prdx1-null mice have abnormalities in NK cell numbers and function. Targeted gene inactivation (knockout mouse), phenotypic analysis including erythrocyte ROS measurement, haemoglobin stability assay, Heinz body staining, NK cell functional assays Nature High 12891360
2009 Prdx1 interacts with the transmembrane protein GDE2 and activates it through reduction of an intramolecular disulfide bond bridging GDE2's intracellular N- and C-terminal domains; this thiol-redox-dependent activation is required for spinal motor neuron differentiation. GDE2 variants incapable of disulfide bond formation are constitutively active and independent of Prdx1, establishing catalytic mechanism. Co-immunoprecipitation, genetic loss-of-function (Prdx1 and GDE2 ablation), active-site mutagenesis of Prdx1 (thiol-dead variants), GDE2 disulfide bond-null mutants, motor neuron differentiation assays in chick spinal cord Cell High 19766572
2013 Prdx1 binds to both MKP-1 and MKP-5 (MAPK phosphatases). When its peroxidatic cysteine Cys52 is over-oxidised to sulfonic acid, Prdx1 dissociates from MKP-1, leading to MKP-1 oxidation-induced oligomerisation and inactivation toward p38MAPKα. Conversely, over-oxidised Prdx1 enhances the Prdx1:MKP-5 complex, protecting MKP-5 from inactivation, thereby maintaining MKP-5 activity toward p38MAPK and controlling ROS-induced senescence in breast epithelial cells. Co-immunoprecipitation, active-site mutant analysis (Cys52), H2O2 dose-response experiments, p38MAPKα activity assays in human breast epithelial cells Oncogene Medium 23334324
2013 Pin1 binds to the phospho-Thr90-Pro91 motif of PRDX1 and facilitates PP2A-mediated dephosphorylation of PRDX1, thereby restoring its peroxidase activity. In Pin1-/- MEFs, PRDX1 accumulates in an inactive phosphorylated form, leading to increased H2O2 and decreased peroxidase activity, which is rescued by re-introduction of Pin1. Proteomic identification of Pin1 binding partners, co-immunoprecipitation, Thr90 mutagenesis, Pin1 knockout MEFs, Pin1 rescue experiments, peroxidase activity assay Cell cycle (Georgetown, Tex.) Medium 23421996
2013 PRDX1 associated with c-Abl kinase under basal conditions; oxidative stress promotes dissociation of the Prdx1:c-Abl complex, leading to c-Abl activation and subsequent caveolin-1 phosphorylation and albumin endocytosis. AMPK activation suppresses this pathway by stabilising the Prdx1:c-Abl interaction. Prdx1 knockdown increased phosphorylation of both c-Abl and caveolin-1 and abolished AMPK's inhibitory effect. Co-immunoprecipitation, siRNA knockdown of Prdx1 and c-Abl, pharmacological AMPK activation/inhibition, caveolin-1 phosphorylation assay, albumin endocytosis assay in HUVEC cells The Journal of biological chemistry Medium 23723070
2018 PRDX1 interacts with the RING finger domain of TRAF6 and inhibits its ubiquitin-ligase activity, thereby suppressing ubiquitination of ECSIT (required for NF-κB activation) and BECN1 (required for autophagy). PRDX1 knockdown in THP-1, MDA-MB-231, and SK-HEP-1 cells increased NF-κB activation, pro-inflammatory cytokines, and enhanced cancer cell migration/invasion. Co-immunoprecipitation (PRDX1 with TRAF6 RING domain), ubiquitination assay for ECSIT and BECN1, PRDX1 knockdown (siRNA), NF-κB reporter assay, cytokine ELISA, migration/invasion assay Autophagy Medium 29929436
2018 PRDX1 and MTH1 cooperate to prevent accumulation of oxidised guanine in the genome. Concomitant disruption of PRDX1 and MTH1 genes in cancer cells leads to ROS-dependent continuous telomere shortening due to inhibition of telomerase-mediated telomere extension by oxidised guanine. CRISPR gene disruption of PRDX1 and MTH1, telomere length measurement, telomerase activity assay, ROS quantification Genes & development High 29773556
2018 PRDX1 forms a heterodimer with p38α (MAPK14), stabilising phospho-p38α in glioma cells. This complex amplifies HGF/MET-driven signalling and promotes actin cytoskeleton dynamics and glioma cell migration in vitro and invasion in vivo. Biochemical interaction studies (co-immunoprecipitation), in vitro and ex vivo migration assays, whole-brain ultramicroscopy, mouse glioma survival models International journal of cancer Medium 29582423
2018 Differential kinetics between PRDX1 and PRDX2: the rate of disulfide formation (resolution step) is 11 s⁻¹ for PRDX1 vs 0.2 s⁻¹ for PRDX2, making PRDX1 less susceptible to hyperoxidation and directing it toward a classical disulfide relay rather than a sulfenic-acid-mediated redox relay used by PRDX2. In vitro kinetic assays with recombinant human PRDX1 and PRDX2, intrinsic fluorescence monitoring of oxidation and hyperoxidation by H2O2 and peroxynitrite Protein science : a publication of the Protein Society High 30284335
2020 PRDX1 is enriched in telomeric chromatin. In PRDX1-depleted cells, PARP-dependent telomeric repair is often incomplete, generating persistent single-strand breaks that are converted into double-strand breaks during replication, causing rapid telomere shortening. PARP1 inhibition dampens this telomere shortening by promoting BRCA1/RAD51-mediated homologous recombination repair. PRDX1 depletion (siRNA/CRISPR), telomeric chromatin ChIP, PARP inhibition, telomere length assays, DNA damage marker assays Cell reports Medium 33147465
2021 PRDX1 oligomers bind with both the Nedd8-conjugating enzyme UBE2F and CUL5, forming a tricomplex critical for CUL5 neddylation. This promotes ubiquitination and degradation of the pro-apoptotic protein NOXA. Silencing PRDX1 or inhibiting PRDX1 oligomerisation greatly dampened CUL5 neddylation and extended the NOXA protein half-life. Co-immunoprecipitation, PRDX1 knockdown, PRDX1 oligomerisation inhibition, NOXA ubiquitination and protein stability assays, CUL5 neddylation assay Cell death & disease Medium 33712558
2021 Prdx1 interacts with ASK1 at elevated H2O2 concentrations independently of a scaffolding protein, constituting a redox-relay. This differs from the Prdx2:STAT3 relay and the Prdx2:ASK1 interaction (which requires a facilitator that is not annexin A2). Co-immunoprecipitation in cells, in vitro protein-protein interaction assays, H2O2 dose-response, comparison with Prdx2 Antioxidants (Basel, Switzerland) Medium 34209102
2022 18β-glycyrrhetinic acid covalently binds to active cysteine residues of PRDX1 (and PRDX2), inhibiting their peroxidase activities, leading to ROS elevation and apoptosis in activated hepatic stellate cells. PRDX1 knockdown alone also produced ROS-mediated apoptosis. Activity-based protein profiling (chemoproteomic), cellular thermal shift assay, surface plasmon resonance, PRDX1 knockdown, enzymatic activity assay Journal of pharmaceutical analysis Medium 36105163
2023 During the DNA damage response, PRDX1 translocates to the nucleus where it reduces DNA damage-induced nuclear ROS. Loss of PRDX1 lowers aspartate availability, impairing DNA damage-induced upregulation of de novo nucleotide synthesis, and causes replication stress accumulation. Functional genomics screen integrated with chromatin proteomics and metabolomics, PRDX1 KO, nuclear fractionation/localisation experiments, aspartate and nucleotide metabolite measurements Molecular systems biology Medium 37259925
2023 LncFASA directly binds to the Ahpc-TSA domain of PRDX1 and drives liquid-liquid phase separation of PRDX1, forming droplets that inhibit its peroxidase activity and disrupt ROS homeostasis, thereby promoting ferroptosis via the SLC7A11-GPX4 axis. RNA-protein binding assay (Ahpc-TSA domain mapping), phase separation droplet assay, PRDX1 peroxidase activity assay, SLC7A11-GPX4 pathway analysis, xenograft tumor model Science China. Life sciences Medium 37955780
2024 MOF acetyltransferase acetylates PRDX1 at lysine 197 (K197), preventing its hyperoxidation and maintaining peroxidase activity under stress. LPS-induced inflammatory signalling rapidly decreases PRDX1 K197 acetylation in macrophages, elevating H2O2 accumulation, augmenting ERK1/2 phosphorylation, stimulating glycolysis, and enhancing pro-inflammatory mediator production (IL-6). Identification of MOF as PRDX1 acetyltransferase, K197 acetylation mass spectrometry, MOF KO macrophages, LPS stimulation, hyperoxidation assays, ERK1/2 phosphorylation, metabolic (glycolysis) assays Cell reports High 39207899
2024 HJURP forms disulfide-linked intermediates with PRDX1 through its Cys327 and Cys457 residues, promoting PRDX1 redox cycling and inhibiting its hyperoxidation, thereby enhancing PRDX1 peroxidase activity and reducing ROS/lipid peroxidation to suppress ferroptosis in prostate cancer cells. Co-immunoprecipitation, disulfide bond formation assay, mutagenesis of HJURP cysteines, PRDX1 peroxidase activity assay, ROS and lipid peroxidation measurements, in vitro and in vivo ferroptosis assays Redox biology Medium 39405980
2024 PRDX1 acts as a molecular chaperone by binding to CUL3, inhibiting CUL3-mediated NRF2 ubiquitination, thereby promoting NRF2 nuclear translocation and transcription of GPX4. This chaperone binding requires PRDX1 Cys83; the Cys83Ser mutant abolishes CUL3 binding. Conoidin A enhances CUL3 binding. IP-mass spectrometry, Co-immunoprecipitation (PRDX1 with CUL3), Cys83Ser mutagenesis, NRF2 ubiquitination assay, PRDX1 KO mice (AOM/DSS CRC model), RNA sequencing, GPX4 transcription assay International journal of biological sciences High 39430237
2024 Hspb1 directly interacts with Anxa2 to decrease its aggregation and phosphorylation, enabling Anxa2 to interact with Prdx1 and maintain its antioxidative activity by decreasing Prdx1 Thr90 phosphorylation. Overexpression of Hspb1 did not protect against pancreatitis in acinar-specific Prdx1 knockout mice, establishing Prdx1 as the downstream effector. Co-immunoprecipitation (Hspb1 with Anxa2 and Prdx1), Anxa2 KO mice, acinar-specific Prdx1 KO mice, Thr90 phosphorylation assay, AAV8-Hspb1 rescue experiments International journal of biological sciences Medium 38481805
2024 PRDX1 directly interacts with the actin-binding protein Cofilin, inhibiting phosphorylation of Cofilin at Ser3, thereby accelerating actin depolymerisation and turnover, promoting oral squamous cell carcinoma cell movement, invasion, and metastasis. Co-immunoprecipitation (PRDX1 with Cofilin), Cofilin Ser3 phosphorylation assay, actin dynamics assay, in vitro migration/invasion assays, nude mouse tongue cancer model International journal of cancer Medium 38738971
2024 IRAK1 binds to PRDX1 and prevents ubiquitination and proteasomal degradation of PRDX1 mediated by the E3 ubiquitin ligase HECTD3. Both the DOC and HECT domains of HECTD3 directly interact with PRDX1. IP/Co-IP, LC-MS/MS, GST pull-down, ubiquitination assay, HECTD3 domain mapping Cell death & disease Medium 37031183
2025 ZNF207 promotes lactylation of PRDX1 at lysine 67, which enhances PRDX1 nuclear translocation and activation of NRF2, creating a ferroptosis-resistant environment and conferring regorafenib resistance in HCC cells. Disrupting PRDX1 K67 lactylation or NRF2 activity reverses resistance. CRISPR/Cas9 screening, lactylation modification assay (K67 site), nuclear fractionation/localisation, NRF2 activity assay, ferroptosis functional assays, drug resistance assays Drug resistance updates Medium 40680452
2025 Artesunate directly binds to PRDX1 (interacting at Gly4) and PRDX2, inhibiting their peroxidase activities and inducing ferroptosis in diffuse large B-cell lymphoma cells. PRDX1 knockdown reproduced ferroptosis and reduced sensitivity to artesunate; PRDX2 overexpression attenuated artesunate-induced ROS and cytotoxicity. Small-molecule pull-down/LC-MS/MS, CETSA, fluorescence titration, circular dichroism, molecular docking, PRDX1/2 knockdown and overexpression, peroxidase activity assay, xenograft model Cell death & disease High 40645965
2025 A co-crystal structure of PRDX1 with the celastrol derivative LC-PDin20, combined with molecular docking, revealed the binding mode of covalent inhibitors to PRDX1 active site, enabling structure-based design of selective PRDX1 inhibitors. Co-crystal structure determination, molecular docking, in vitro PRDX1 enzymatic inhibition assay (IC50 determination), cell antiproliferation assay Journal of medicinal chemistry High 40546088
2017 Prdx1 deficiency impairs lipophagic flux in macrophages by causing excessive oxidative stress, leading to reduced free cholesterol formation, decreased NR1H3 (LXRα) activity, and lower cholesterol efflux. 2-Cys PRDX mimics ebselen and gliotoxin restored both lipophagic flux and cholesterol efflux in Prdx1-deficient macrophages. Prdx1 KO macrophages, autophagic flux assay, cholesterol efflux assay, NR1H3 activity assay, ebselen/gliotoxin rescue, bone marrow transplant into apoe-/- mice with plaque measurement Autophagy Medium 28605287
2019 PRDX1 inhibits the activated (cancer-associated) fibroblast phenotype by binding to JNK1 and regulating JNK kinase signalling; loss of PRDX1 results in development of a CAF-like phenotype in mammary fibroblasts. JNK inhibition with SP600125 reduced CAF-like behaviors in Prdx1 KO fibroblasts. Co-immunoprecipitation (PRDX1 with JNK1), PRDX1 KO mouse fibroblasts, JNK inhibitor (SP600125), transwell migration/invasion assay, immunofluorescence BMC cancer Medium 31419957
2021 PRDX1 activates autophagy in spiral ganglion neurons at least partially through activation of the PTEN-AKT signalling pathway; PRDX1 deficiency suppresses autophagy and increases neuronal loss after cisplatin exposure, while PRDX1 upregulation (pharmacologically or by AAV) activates autophagy and reduces ROS accumulation. PRDX1 KO and AAV-mediated overexpression, autophagy flux assay (LC3B, p62), PTEN/AKT pathway western blot, ROS measurement, SGN survival and hearing function assays Autophagy Medium 33749526
2023 PRDX1 Cys52Ser (peroxidase-dead) variant mice show impaired global PRDX peroxidase activity and reduced susceptibility to diet-induced NASH and liver fibrosis. Mechanistically, the Cys52Ser variant suppresses NF-κB and STAT1 signalling, indicating that PRDX1 peroxidatic Cys52 is required for its pro-inflammatory activity in vivo. Knock-in mouse (PRDX1 Cys52Ser), Trx-TrxR-NADPH coupled peroxidase activity assay, western diet/MCD diet NASH model, RNA sequencing, NF-κB and STAT1 pathway analysis Molecular metabolism High 37562742
2024 PRDX1 interacts with DOK3 and modulates DOK3 degradation via the autophagy-lysosome pathway, thereby inhibiting plasma cell differentiation. The small molecule Salvianolic acid B acts as a molecular glue enhancing the PRDX1-DOK3 interaction, further impairing plasma cell differentiation and collagen-induced arthritis progression. Co-immunoprecipitation (PRDX1-DOK3), autophagy-lysosome pathway inhibition assay, plasma cell differentiation assay, collagen-induced arthritis mouse model, small molecule (SAB) functional validation Acta pharmaceutica Sinica. B Medium 40893682
2023 Modelling shows that the PRDX1 dimer-to-decamer transition has an inhibition-like effect on peroxidase activity; association and dissociation rate constants of 0.050 µM⁻⁴·s⁻¹ and 0.055 s⁻¹ respectively were obtained from isothermal titration calorimetry data and incorporated into kinetic models. Kinetic modelling using isothermal titration calorimetry data, HRP competition assay simulation, NADPH-oxidation linked assay simulation Antioxidants (Basel, Switzerland) Low 37760010
2025 BACH1 directly binds to the PRDX1 promoter region and inhibits PRDX1 transcription. Remifentanil treatment inactivates BACH1, relieving PRDX1 repression and thereby reducing oxidative stress in hepatic ischemia-reperfusion injury. Chromatin immunoprecipitation (ChIP), dual luciferase reporter assay, PRDX1 silencing rescue, HIRI mouse model, mRNA microarray Clinics and research in hepatology and gastroenterology Medium 39025461

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells. Genome biology 1178 30890159
2003 Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression. Nature 649 12891360
2021 PRDX1 activates autophagy via the PTEN-AKT signaling pathway to protect against cisplatin-induced spiral ganglion neuron damage. Autophagy 189 33749526
2018 Inhibition of TRAF6 ubiquitin-ligase activity by PRDX1 leads to inhibition of NFKB activation and autophagy activation. Autophagy 186 29929436
2009 The antioxidant enzyme Prdx1 controls neuronal differentiation by thiol-redox-dependent activation of GDE2. Cell 94 19766572
2008 Proteomic expression analysis of surgical human colorectal cancer tissues: up-regulation of PSB7, PRDX1, and SRP9 and hypoxic adaptation in cancer. Journal of proteome research 89 18549262
2002 HIV-1 antiviral activity of recombinant natural killer cell enhancing factors, NKEF-A and NKEF-B, members of the peroxiredoxin family. The Journal of biological chemistry 81 12421812
2018 Prdx1 alleviates cardiomyocyte apoptosis through ROS-activated MAPK pathway during myocardial ischemia/reperfusion injury. International journal of biological macromolecules 77 29410271
2017 Prdx1 (peroxiredoxin 1) deficiency reduces cholesterol efflux via impaired macrophage lipophagic flux. Autophagy 77 28605287
2013 Role for Prdx1 as a specific sensor in redox-regulated senescence in breast cancer. Oncogene 74 23334324
2021 PRDX-1 Supports the Survival and Antitumor Activity of Primary and CAR-Modified NK Cells under Oxidative Stress. Cancer immunology research 66 34853030
2018 PRDX1 and MTH1 cooperate to prevent ROS-mediated inhibition of telomerase. Genes & development 64 29773556
2018 FGF1 improves functional recovery through inducing PRDX1 to regulate autophagy and anti-ROS after spinal cord injury. Journal of cellular and molecular medicine 57 29512938
2005 Histone deacetylase inhibitor FK228 activates tumor suppressor Prdx1 with apoptosis induction in esophageal cancer cells. Clinical cancer research : an official journal of the American Association for Cancer Research 54 16278420
2023 LncFASA promotes cancer ferroptosis via modulating PRDX1 phase separation. Science China. Life sciences 45 37955780
2022 18beta-glycyrrhetinic acid induces ROS-mediated apoptosis to ameliorate hepatic fibrosis by targeting PRDX1/2 in activated HSCs. Journal of pharmaceutical analysis 44 36105163
2021 Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1. Acta pharmacologica Sinica 44 33833406
2006 Cloning and characterisation of two natural killer enhancing factor genes (NKEF-A and NKEF-B) in pufferfish, Tetraodon nigroviridis. Fish & shellfish immunology 43 16690325
2023 Dual role of PRDX1 in redox-regulation and tumorigenesis: Past and future. Free radical biology & medicine 42 37977211
2018 Differential parameters between cytosolic 2-Cys peroxiredoxins, PRDX1 and PRDX2. Protein science : a publication of the Protein Society 42 30284335
2021 Cullin-5 neddylation-mediated NOXA degradation is enhanced by PRDX1 oligomers in colorectal cancer. Cell death & disease 39 33712558
2013 AMP-dependent kinase inhibits oxidative stress-induced caveolin-1 phosphorylation and endocytosis by suppressing the dissociation between c-Abl and Prdx1 proteins in endothelial cells. The Journal of biological chemistry 38 23723070
2013 PAG--a multipurpose transmembrane adaptor protein. Oncogene 37 24213579
2011 Downregulation of PRDX1 by promoter hypermethylation is frequent in 1p/19q-deleted oligodendroglial tumours and increases radio- and chemosensitivity of Hs683 glioma cells in vitro. Oncogene 37 22158042
1994 Organization and chromosomal assignment of two human PAG gene loci: PAGA encoding a functional gene and PAGB a processed pseudogene. Genomics 37 8188254
2019 PRDX1 enhances cerebral ischemia-reperfusion injury through activation of TLR4-regulated inflammation and apoptosis. Biochemical and biophysical research communications 36 31526567
2024 Hspb1 protects against severe acute pancreatitis by attenuating apoptosis and ferroptosis via interacting with Anxa2 to restore the antioxidative activity of Prdx1. International journal of biological sciences 35 38481805
2016 Identification and verification of PRDX1 as an inflammation marker for colorectal cancer progression. American journal of translational research 35 27158373
2020 PRDX1 Counteracts Catastrophic Telomeric Cleavage Events That Are Triggered by DNA Repair Activities Post Oxidative Damage. Cell reports 34 33147465
2022 HBXIP induces anoikis resistance by forming a reciprocal feedback loop with Nrf2 to maintain redox homeostasis and stabilize Prdx1 in breast cancer. NPJ breast cancer 33 35027562
2023 Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells. Cell death & disease 32 37031183
2023 A metabolic map of the DNA damage response identifies PRDX1 in the control of nuclear ROS scavenging and aspartate availability. Molecular systems biology 31 37259925
2023 PRDX1 negatively regulates bleomycin-induced pulmonary fibrosis via inhibiting the epithelial-mesenchymal transition and lung fibroblast proliferation in vitro and in vivo. Cellular & molecular biology letters 30 37268886
2014 Proteomic analysis of lung tissue in a rat acute lung injury model: identification of PRDX1 as a promoter of inflammation. Mediators of inflammation 30 25024510
2013 PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms. International journal of oncology 28 24316730
2023 The super-enhancer-driven lncRNA LINC00880 acts as a scaffold between CDK1 and PRDX1 to sustain the malignance of lung adenocarcinoma. Cell death & disease 27 37620336
2015 PRDX1 is involved in palmitate induced insulin resistance via regulating the activity of p38MAPK in HepG2 cells. Biochemical and biophysical research communications 26 26301632
2012 Prdx1 deficiency in mice promotes tissue specific loss of heterozygosity mediated by deficiency in DNA repair and increased oxidative stress. Mutation research 26 22583657
2012 Proteomics in HepG2 hepatocarcinoma cells with stably silenced expression of PRDX1. Journal of proteomics 26 23277276
2020 Prdx1 Reduces Intracerebral Hemorrhage-Induced Brain Injury via Targeting Inflammation- and Apoptosis-Related mRNA Stability. Frontiers in neuroscience 24 32210752
2017 Prdx1-encoded peroxiredoxin is important for vascular development in zebrafish. FEBS letters 24 28231391
2024 PRDX1 inhibits ferroptosis by binding to Cullin-3 as a molecular chaperone in colorectal cancer. International journal of biological sciences 23 39430237
2005 Transformation of an edible crop with the pagA gene of Bacillus anthracis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23 16030177
2013 Regulation of PRDX1 peroxidase activity by Pin1. Cell cycle (Georgetown, Tex.) 22 23421996
2025 Discovery of Natural Resorcylic Acid Lactones as Novel Potent Copper Ionophores Covalently Targeting PRDX1 to Induce Cuproptosis for Triple-Negative Breast Cancer Therapy. ACS central science 20 40028362
2024 Matairesinol blunts adverse cardiac remodeling and heart failure induced by pressure overload by regulating Prdx1 and PI3K/AKT/FOXO1 signaling. Phytomedicine : international journal of phytotherapy and phytopharmacology 20 39306883
2020 PRDX1 stimulates non-small-cell lung carcinoma to proliferate via the Wnt/β-Catenin signaling. Panminerva medica 20 32881473
2019 The peroxidase PRDX1 inhibits the activated phenotype in mammary fibroblasts through regulating c-Jun N-terminal kinases. BMC cancer 20 31419957
2019 Bacillus anthracis Virulence Regulator AtxA Binds Specifically to the pagA Promoter Region. Journal of bacteriology 20 31570528
2018 Global analysis of erythroid cells redox status reveals the involvement of Prdx1 and Prdx2 in the severity of beta thalassemia. PloS one 20 30521599
2025 ZNF207-driven PRDX1 lactylation and NRF2 activation in regorafenib resistance and ferroptosis evasion. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 19 40680452
2024 HJURP inhibits sensitivity to ferroptosis inducers in prostate cancer cells by enhancing the peroxidase activity of PRDX1. Redox biology 19 39405980
2020 Prdx1 promotes the loss of primary cilia in esophageal squamous cell carcinoma. BMC cancer 18 32357862
2015 Identification of RAB2A and PRDX1 as the potential biomarkers for oral squamous cell carcinoma using mass spectrometry-based comparative proteomic approach. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 18 26159854
2022 PRDX1 Influences The Occurrence and Progression of Liver Cancer by Inhibiting Mitochondrial Apoptosis Pathway. Cell journal 17 36377215
2020 PRDX1 is a Tumor Suppressor for Nasopharyngeal Carcinoma by Inhibiting PI3K/AKT/TRAF1 Signaling. OncoTargets and therapy 17 32982301
2024 MOF-mediated PRDX1 acetylation regulates inflammatory macrophage activation. Cell reports 16 39207899
2018 BMP signaling-driven osteogenesis is critically dependent on Prdx-1 expression-mediated maintenance of chondrocyte prehypetrophy. Free radical biology & medicine 16 29452246
2021 PRDX1 gene-related epi-cblC disease is a common type of inborn error of cobalamin metabolism with mono- or bi-allelic MMACHC epimutations. Clinical epigenetics 15 34215320
2019 Decreased expression of microRNA-510 in intestinal tissue contributes to post-infectious irritable bowel syndrome via targeting PRDX1. American journal of translational research 15 31934286
2023 Chronic exposure to low-dose deltamethrin can lead to colon tissue injury through PRDX1 inactivation-induced mitochondrial oxidative stress injury and gut microbial dysbiosis. Ecotoxicology and environmental safety 14 37714033
2020 Inhibition of the antioxidant enzyme PRDX1 activity promotes MPP+-induced death in differentiated SH-SY5Y cells and may impair its colocalization with eEF1A2. Life sciences 14 32781074
2017 l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. Theriogenology 14 28965027
2024 Furanodienone induces apoptosis via regulating the PRDX1/MAPKs/p53/caspases signaling axis through NOX4-derived mitochondrial ROS in colorectal cancer cells. Biochemical pharmacology 13 39079582
2023 PRDX1 Cys52Ser variant alleviates nonalcoholic steatohepatitis by reducing inflammation in mice. Molecular metabolism 13 37562742
2022 miR-375 suppresses the growth and metastasis of esophageal squamous cell carcinoma by targeting PRDX1. Journal of gastrointestinal oncology 13 36388649
2021 Prdx1 Interacts with ASK1 upon Exposure to H2O2 and Independently of a Scaffolding Protein. Antioxidants (Basel, Switzerland) 12 34209102
2018 A PRDX1-p38α heterodimer amplifies MET-driven invasion of IDH-wildtype and IDH-mutant gliomas. International journal of cancer 12 29582423
2021 PRDX1 is essential for the viability and maintenance of reactive oxygen species in chicken DT40. Genes and environment : the official journal of the Japanese Environmental Mutagen Society 10 34353368
2018 Peroxiredoxin 1 (PRDX1) Suppresses Progressions and Metastasis of Osteosarcoma and Fibrosarcoma of Bone. Medical science monitor : international medical journal of experimental and clinical research 10 29908016
2012 Identification of proteins containing redox-sensitive thiols after PRDX1, PRDX3 and GCLC silencing and/or glucose oxidase treatment in Hepa 1-6 cells. Journal of proteomics 10 22975676
2025 Artesunate induces ferroptosis in diffuse large B-cell lymphoma cells by targeting PRDX1 and PRDX2. Cell death & disease 9 40645965
2023 TDP43/HDAC6/Prdx1 signaling pathway participated in the cognitive impairment of obstructive sleep apnea via regulating inflammation and oxidative stress. International immunopharmacology 9 38104368
2011 [Effects of yiqi chutan recipe on tumor growth, survival time and expressions of PRDX-1 and PRDX-6 in Lewis lung carcinoma model mice with pi-deficiency syndrome]. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine 8 21434354
2024 The interaction of PRDX1 with Cofilin promotes oral squamous cell carcinoma metastasis. International journal of cancer 7 38738971
2024 PRDX1 Interfering Peptide Disrupts Amino Acids 70-90 of PRDX1 to Inhibit the TLR4/NF-κB Signaling Pathway and Attenuate Neuroinflammation and Ischemic Brain Injury. Molecular neurobiology 7 38780721
2023 Hepatocytes-derived Prdx1 regulates macrophage phenotypes via TLR4 activation in acute liver injury. International immunopharmacology 7 38159556
2025 Rapid Discovery of Celastrol Derivatives as Potent and Selective PRDX1 Inhibitors via Microplate-Based Parallel Compound Library and In Situ Screening. Journal of medicinal chemistry 6 40546088
2025 Tumor-associated bacteria activate PRDX1-driven glycolysis to promote immune evasion and PD-1 antibody resistance in hepatocellular carcinoma. Frontiers in microbiology 6 40693141
2005 Rare allelic imbalances, but no mutations of the PRDX1 gene in human hepatocellular carcinomas. Journal of clinical pathology 6 16254121
2025 PRDX1 knockdown promotes erastin-induced ferroptosis and impedes diffuse large B-cell lymphoma development by inhibiting the MAPK/ERK pathway. BMC cancer 5 40307771
2024 PRDX1 exerts a photoprotection effect by inhibiting oxidative stress and regulating MAPK signaling on retinal pigment epithelium. BMC ophthalmology 5 38844903
2024 Remifentanil represses oxidative stress to relieve hepatic ischemia/reperfusion injury via regulating BACH1/PRDX1 axis. Clinics and research in hepatology and gastroenterology 5 39025461
2024 Antioxidant enzyme Prdx1 inhibits osteoclastogenesis via suppressing ROS and NFATc1 signaling pathways. Journal of cellular physiology 5 39263840
2023 Altered Regulation of the Glucose Transporter GLUT3 in PRDX1 Null Cells Caused Hypersensitivity to Arsenite. Cells 5 38067110
2021 Fish natural killer enhancing factor-A (NKEF-A) enhance cytotoxicity of nonspecific cytotoxic cells against bacterial infection. Molecular immunology 5 33744653
2025 Glaucocalyxin A induces autophagy-mediated ferroptosis by targeting PRDX1 and TXNRD1 proteins in non-small cell lung cancer. Phytomedicine : international journal of phytotherapy and phytopharmacology 4 41016297
2024 Molecular characterization, cytoprotective, DNA protective, and immunological assessment of peroxiredoxin-1 (Prdx1) from yellowtail clownfish (Amphiprion clarkii). Developmental and comparative immunology 4 38574831
2023 MicroRNA-146a Improved Acute Lung Injury Induced by hepatic Ischemia-reperfusion Injury by Inhibiting PRDX1. Dose-response : a publication of International Hormesis Society 4 37063344
2023 Effects of Date Palm Pollen Supplementations on The Expression of PRDX1 and PRDX6 Genes in Infertile Men: A Controlled Clinical Trial. International journal of fertility & sterility 4 37183847
2023 Modelling the Decamerisation Cycle of PRDX1 and the Inhibition-like Effect on Its Peroxidase Activity. Antioxidants (Basel, Switzerland) 4 37760010
2025 METTL3-mediated NFAT5 Upregulation Promotes Cervical Cancer Progression Through Enhancing the Mitochondrial Function by Transcriptional Regulation of PRDX1. Journal of biochemical and molecular toxicology 3 39925026
2025 Panoptosis Targets the Mechanism of PRDX1 Involvement in Acute Myocardial Infarction via ZBP1. Journal of cellular biochemistry 3 40114419
2025 Augmentation of PRDX1-DOK3 interaction alleviates rheumatoid arthritis progression by suppressing plasma cell differentiation. Acta pharmaceutica Sinica. B 3 40893682
2025 Investigating the potential causal link between BPA and ovarian carcinogenesis: a network toxicology and mendelian randomization study on the CTRC/PRDX1/SKP1 pathway. Journal of ovarian research 3 41108004
2022 A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system. STAR protocols 3 35284843
2020 [Effect and mechanism of PRDX1 in epithelial mesenchymal transformationin of gastric cancer cells]. Zhonghua zhong liu za zhi [Chinese journal of oncology] 3 33256302
2025 l-Glutamic Acid Alleviates Mastitis in Dairy Cows by Targeting the Sirt5/Nrf2/Prdx1 Signaling Axis and Mitochondrial Function. Journal of agricultural and food chemistry 2 39895052
2025 PRDX1 affects acrylamide-induced neural damage through the PTEN/AKT signaling pathway. Neurotoxicology 2 40189060
2025 Targeting PRDX1 impairs acute myeloid leukemic blasts and stem cells by disrupting redox homeostasis. Cell death & disease 2 40825764

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