Affinage

PRDX1

Peroxiredoxin-1 · UniProt Q06830

Length
199 aa
Mass
22.1 kDa
Annotated
2026-04-28
100 papers in source corpus 31 papers cited in narrative 31 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PRDX1 is a 2-Cys peroxiredoxin that functions as a central redox sensor and signal relay hub, coupling intracellular H₂O₂ levels to diverse signaling, transcriptional, and genome-protective outputs. Its peroxidatic cysteine Cys52 reduces H₂O₂ with rapid kinetics and undergoes dose-dependent oxidation states—including hyperoxidation to sulfonic acid—that switch PRDX1 between peroxidase, redox relay, and molecular chaperone modes, controlling partner interactions with MKP-1/MKP-5, ASK1, JNK1, TRAF6, CUL3, UBE2F-CUL5, p38α, Cofilin, and GDE2 to regulate MAP kinase signaling, NF-κB activation, autophagy, NRF2 stability, apoptosis, cytoskeletal dynamics, and motor neuron differentiation (PMID:30284335, PMID:23334324, PMID:29929436, PMID:39430237, PMID:19766572, PMID:33712558, PMID:29582423, PMID:38738971). Post-translational modifications—including Thr90 phosphorylation regulated by Pin1/PP2A, K197 acetylation by MOF, and K67 lactylation—tune its enzymatic activity, oligomeric state, and subcellular distribution, with nuclear translocation upon DNA damage enabling PRDX1 to scavenge nuclear ROS, support de novo nucleotide synthesis, localize to telomeric chromatin to prevent oxidative telomere damage, and sustain telomerase activity (PMID:23421996, PMID:39207899, PMID:40680452, PMID:37259925, PMID:33147465, PMID:29773556). Prdx1 knockout mice develop severe hemolytic anemia from erythrocyte oxidative damage, increased cancer susceptibility, and NK cell dysfunction, while Cys52Ser knock-in mice are protected from diet-induced steatohepatitis, demonstrating that PRDX1 peroxidase activity has both cytoprotective and context-dependent pro-inflammatory roles in vivo (PMID:12891360, PMID:37562742).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2003 High

    Establishing the in vivo requirement for PRDX1: knockout mice revealed that PRDX1 is not redundant with other peroxiredoxins but is essential for erythrocyte redox homeostasis, proliferative capacity, and immune cell function, defining it as a non-redundant antioxidant defense.

    Evidence Targeted Prdx1 knockout mice with hematological, ROS, and NK cell phenotyping

    PMID:12891360

    Open questions at the time
    • Mechanism of selective erythrocyte vulnerability versus other cell types not resolved
    • Contribution of chaperone versus peroxidase activity to the KO phenotype not distinguished
  2. 2009 High

    Demonstrating that PRDX1 acts as a thiol-based redox switch for a specific biological effector: PRDX1 activates GDE2 by reducing an intramolecular disulfide bond, establishing that PRDX1's catalytic activity directly couples to a developmental signaling event (motor neuron differentiation).

    Evidence Co-IP, active-site mutagenesis, Prdx1 knockdown/KO in chick spinal cord with epistasis analysis

    PMID:19766572

    Open questions at the time
    • Whether PRDX1-GDE2 interaction is constitutive or regulated by ROS levels in vivo not tested
    • Other developmental signals requiring PRDX1 redox relay not mapped
  3. 2013 High

    Revealing how PRDX1's oxidation state enables dose-dependent H₂O₂ signaling: PRDX1 differentially associates with MKP-1 and MKP-5 depending on Cys52 oxidation status, coordinating p38 MAPK-driven senescence and establishing PRDX1 as a redox relay with partner-selective outputs.

    Evidence Cys52 mutagenesis, H₂O₂ dose-response binding assays, p38 MAPK phosphorylation readout in breast epithelial cells

    PMID:23334324

    Open questions at the time
    • Whether intermediate oxidation states of Cys52 generate additional signaling specificity not tested
    • In vivo senescence phenotype in PRDX1-deficient tissues not examined
  4. 2013 High

    Identifying post-translational control of PRDX1 peroxidase activity: Pin1 recognizes phospho-Thr90 and recruits PP2A for dephosphorylation, restoring enzymatic activity, thus defining a phospho-switch that gates PRDX1 function.

    Evidence Proteomic pulldown, Thr90 mutagenesis, peroxidase activity rescue in Pin1-KO MEFs

    PMID:23421996

    Open questions at the time
    • Kinase responsible for Thr90 phosphorylation not definitively identified in this study
    • Whether Thr90 phosphorylation also regulates chaperone or relay functions not tested
  5. 2018 High

    Establishing PRDX1 as a negative regulator of innate immune signaling and autophagy through direct E3 ligase inhibition: PRDX1 binds the RING domain of TRAF6 and blocks ubiquitination of ECSIT and BECN1, suppressing both NF-κB activation and autophagy induction.

    Evidence Reciprocal Co-IP with domain mapping, ubiquitination assays, PRDX1 knockdown in THP-1 and other cell lines with TLR4 stimulation

    PMID:29929436

    Open questions at the time
    • Whether PRDX1 oxidation state modulates TRAF6 binding affinity not determined
    • Structural basis of RING domain recognition not resolved
  6. 2018 High

    Defining PRDX1 as a guardian of telomere integrity: combined PRDX1/MTH1 disruption causes ROS-dependent telomere shortening by inhibiting telomerase extension, revealing cooperative oxidative damage prevention at telomeres.

    Evidence CRISPR double knockout, telomere length assays, telomerase extension assays

    PMID:29773556

    Open questions at the time
    • Whether PRDX1 is physically present at telomeres was not shown in this study (addressed in 2020)
  7. 2018 High

    Quantitative kinetic analysis established the mechanistic basis for PRDX1's redox-relay versus peroxide-scavenging roles: PRDX1's ~55-fold faster disulfide formation rate compared to PRDX2, plus the additional Cys83 residue, explains its preferential function as a redox relay rather than a peroxide sensor.

    Evidence Recombinant protein kinetic assays with intrinsic fluorescence monitoring

    PMID:30284335

    Open questions at the time
    • In vivo relevance of the kinetic difference between PRDX1 and PRDX2 not directly tested
    • Role of Cys83 in partner-specific relay not investigated
  8. 2020 High

    Direct demonstration of PRDX1 at telomeric chromatin: PRDX1 enrichment at telomeres by ChIP, and epistasis with PARP inhibition, showed that PRDX1 prevents oxidative single-strand breaks that convert to replication-coupled double-strand breaks, mechanistically explaining how PRDX1 loss drives telomere shortening.

    Evidence Telomere ChIP, PRDX1 depletion, PARP inhibitor epistasis, SSB/DSB quantification

    PMID:33147465

    Open questions at the time
    • How PRDX1 is recruited to telomeric chromatin is unknown
    • Whether PRDX1's telomeric role requires its peroxidase or chaperone activity not distinguished
  9. 2020 Medium

    Expanding PRDX1's repertoire beyond protein interactions: RIP-seq identified PRDX1 as an RNA-binding protein that stabilizes inflammation- and apoptosis-related mRNAs, adding post-transcriptional gene regulation to its functional portfolio.

    Evidence RIP-seq in rat brain, AAV-mediated overexpression in ICH model

    PMID:32210752

    Open questions at the time
    • RNA-binding domain/motif not identified
    • Whether RNA binding is redox-dependent not tested
    • Replication in non-CNS systems lacking
  10. 2021 High

    Connecting PRDX1's TRAF6/BECN1 interaction to lipid metabolism: PRDX1 deficiency impairs autophagic cholesterol hydrolysis in macrophages, increasing atherosclerotic plaque formation, establishing a physiological consequence of the PRDX1-TRAF6-BECN1 axis.

    Evidence Prdx1 KO macrophages, lipophagic flux and cholesterol efflux assays, bone marrow transplant in Apoe-KO mice, pharmacological rescue with 2-Cys PRDX mimics

    PMID:28605287

    Open questions at the time
    • Whether PRDX1 promotes or inhibits BECN1 function remains context-dependent and requires clarification across cell types
  11. 2021 Medium

    Revealing a non-enzymatic scaffolding role: PRDX1 oligomers bridge UBE2F and CUL5 to promote CUL5 neddylation and NOXA degradation, linking ROS-driven PRDX1 oligomerization to apoptosis resistance.

    Evidence Co-IP, oligomerization assays, NOXA half-life measurement in colorectal cancer cells

    PMID:33712558

    Open questions at the time
    • Whether oligomeric versus dimeric PRDX1 has distinct partner selectivity beyond UBE2F-CUL5 not tested
    • Independent replication needed
  12. 2023 High

    Demonstrating nuclear translocation as a DNA damage response: PRDX1 moves to the nucleus upon genotoxic stress to scavenge nuclear ROS and support aspartate-dependent de novo nucleotide synthesis, linking its antioxidant activity to replication stress resistance.

    Evidence CRISPR screen, chromatin proteomics, metabolomics, nuclear fractionation after etoposide treatment

    PMID:37259925

    Open questions at the time
    • Signal or modification triggering nuclear import not identified
    • Whether nuclear PRDX1 engages distinct relay partners not explored
  13. 2023 High

    Separating peroxidase-dependent functions in vivo: Cys52Ser knock-in mice showed that the peroxidatic cysteine is specifically required for pro-inflammatory NF-κB and STAT1 signaling in steatohepatitis, demonstrating that PRDX1's disease-promoting roles depend on catalytic redox cycling rather than chaperone activity.

    Evidence Cys52Ser knock-in mouse, Western/MCD diet, RNA-seq, NF-κB/STAT1 pathway analysis

    PMID:37562742

    Open questions at the time
    • Whether chaperone-only functions (e.g., CUL3 binding via Cys83) are preserved in Cys52Ser mice not assessed
  14. 2024 High

    Identifying acetylation as a rapid inflammatory switch: MOF-mediated K197 acetylation protects PRDX1 from hyperoxidation; LPS triggers deacetylation, elevating H₂O₂ to activate ERK1/2 and glycolysis for pro-inflammatory cytokine production, revealing how innate immune activation is coupled to PRDX1 inactivation.

    Evidence MOF KO/knockdown, K197 site identification, LPS stimulation with H₂O₂, ERK1/2, and metabolic flux readouts

    PMID:39207899

    Open questions at the time
    • Deacetylase responsible for LPS-induced K197 deacetylation not identified
    • Interplay between K197 acetylation and Thr90 phosphorylation not explored
  15. 2024 High

    Defining a chaperone-mode interaction with CUL3 that stabilizes NRF2: PRDX1 binds CUL3 via Cys83 (not Cys52), blocking NRF2 ubiquitination and driving GPX4 transcription, demonstrating that PRDX1 has separable catalytic-cysteine and non-catalytic-cysteine dependent partner interactions.

    Evidence IP-MS, Cys83Ser mutagenesis, ubiquitination assays, NRF2 translocation, AOM/DSS mouse model

    PMID:39430237

    Open questions at the time
    • Whether Cys83 oxidation state regulates CUL3 binding not determined
    • Structural basis of the PRDX1-CUL3 interface not resolved
  16. 2025 High

    Obtaining the first co-crystal structure of PRDX1 with a covalent inhibitor provided atomic-resolution insight into the active-site architecture and confirmed covalent modification of catalytic cysteines as the inhibitory mechanism.

    Evidence X-ray co-crystallography with celastrol derivative LC-PDin20, enzymatic IC₅₀ determination

    PMID:40546088

    Open questions at the time
    • No co-crystal structure with a physiological partner (relay target) yet available
    • Whether inhibitor binding disrupts oligomerization not addressed

Open questions

Synthesis pass · forward-looking unresolved questions
  • Major open questions include: how PRDX1 is recruited to specific chromatin loci (telomeres, damage sites); the identity of kinases and deacetylases regulating its PTM switches; structural models of PRDX1 in complex with relay partners; and how the competing peroxidase, chaperone, RNA-binding, and relay activities are partitioned in a single cell.
  • No structural model of PRDX1 bound to any relay partner
  • Signal routing rules between simultaneous partners unknown
  • RNA-binding mechanism and domain uncharacterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016209 antioxidant activity 7 GO:0098772 molecular function regulator activity 6 GO:0003723 RNA binding 1 GO:0044183 protein folding chaperone 1 GO:0140096 catalytic activity, acting on a protein 1
Localization
GO:0005829 cytosol 4 GO:0005634 nucleus 2 GO:0005694 chromosome 2
Pathway
R-HSA-8953897 Cellular responses to stimuli 5 R-HSA-162582 Signal Transduction 4 R-HSA-168256 Immune System 4 R-HSA-73894 DNA Repair 3 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-5357801 Programmed Cell Death 2 R-HSA-9612973 Autophagy 2
Partners
BECN1CFL1CUL3GDE2JNK1MAPK14PIN1TRAF6

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 Prdx1 knockout mice develop severe haemolytic anaemia characterized by increased erythrocyte ROS, protein oxidation, haemoglobin instability, Heinz body formation, and decreased erythrocyte lifespan, establishing PRDX1 as an essential antioxidant defence in erythrocytes. Prdx1-deficient fibroblasts show decreased proliferation and increased sensitivity to oxidative DNA damage, and Prdx1-null mice have abnormalities in NK cell numbers, phenotype, and function. Targeted gene knockout in mice with phenotypic characterization (ROS measurement, haematological assays, tumour histopathology, NK cell functional assays) Nature High 12891360
2009 PRDX1 physically interacts with the transmembrane protein GDE2 and activates it by reducing an intramolecular disulfide bond bridging GDE2's intracellular N- and C-terminal domains, thereby driving spinal motor neuron differentiation. This activation requires PRDX1's thiol-dependent catalytic activity, and GDE2 variants incapable of disulfide bond formation become independent of PRDX1. Co-immunoprecipitation, in vivo loss-of-function (Prdx1 knockdown/knockout in chick spinal cord), active-site mutagenesis of PRDX1 and GDE2, epistasis analysis Cell High 19766572
2013 PRDX1 binds to both MKP-1 and MKP-5 phosphatases in an H2O2 dose-dependent manner via its peroxidatic cysteine Cys52. At high H2O2, over-oxidation of Cys52 to sulfonic acid causes PRDX1 to dissociate from MKP-1, leading to MKP-1 oxidation-induced oligomerization and inactivation toward p38MAPKα, while the PRDX1:MKP-5 complex is stabilized, protecting MKP-5 from inactivation. This mechanism coordinates p38MAPK-dependent cellular senescence in breast epithelial cells. Co-immunoprecipitation, H2O2 dose-response assays, Cys52 mutagenesis, p38MAPK phosphorylation assays, ROS-induced senescence model Oncogene High 23334324
2018 PRDX1 interacts with the RING finger domain of TRAF6 and inhibits its ubiquitin-ligase activity, thereby suppressing ubiquitination of ECSIT (required for NF-κB activation) and BECN1 (required for autophagy). PRDX1 knockdown increases NF-κB activation, pro-inflammatory cytokine production, and bactericidal autophagy. Co-immunoprecipitation, ubiquitination assays in PRDX1-knockdown cell lines (THP-1, MDA-MB-231, SK-HEP-1), TLR4 stimulation experiments Autophagy High 29929436
2018 PRDX1 and MTH1 cooperate to prevent accumulation of oxidized guanine in the genome. Concomitant disruption of PRDX1 and MTH1 in cancer cells causes ROS-dependent continuous telomere shortening due to efficient inhibition of telomere extension by telomerase, identifying PRDX1 as a guardian of telomere integrity. CRISPR gene disruption, telomere length measurement, ROS quantification, telomerase extension assays Genes & development High 29773556
2018 Kinetic analysis established that the peroxidatic cysteine of PRDX1 reacts ~10-fold faster with H2O2 than peroxynitrite (both orders of magnitude faster than typical protein thiols), and that PRDX1 has a substantially faster disulfide formation rate (11 s⁻¹) than PRDX2 (0.2 s⁻¹), which determines its distinct redox-relay function versus PRDX2's mixed-disulfide signaling mode. The additional C83 residue in PRDX1 further differentiates its response to peroxynitrite. In vitro kinetic assays using recombinant human PRDX1 and PRDX2, intrinsic fluorescence monitoring of oxidation and hyperoxidation Protein science High 30284335
2013 Pin1 binds PRDX1 through the phospho-Thr90-Pro91 motif and facilitates PP2A-mediated dephosphorylation of PRDX1, restoring its peroxidase activity. In Pin1-knockout MEFs, phosphorylated (Thr90) PRDX1 accumulates in its inactive form, leading to increased H2O2 buildup. Proteomic pulldown, co-immunoprecipitation, Thr90 mutagenesis, peroxidase activity assay in Pin1−/− MEFs with Pin1 re-introduction, H2O2 measurement Cell cycle High 23421996
2020 PRDX1 is enriched in telomeric chromatin and counteracts ROS-induced telomere damage. In PRDX1-depleted cells, PARP-dependent telomeric repair is often incomplete, generating persistent single-strand breaks that are converted to double-strand breaks during replication. PARP1 inhibition in PRDX1-depleted cells dampens telomere shortening by stabilising BRCA1 and enabling RAD51-mediated HR repair. PRDX1 depletion, telomere ChIP showing PRDX1 enrichment, PARP inhibitor epistasis, telomere length assays, SSB/DSB quantification Cell reports High 33147465
2021 PRDX1 binds TRAF6 and BECN1 and deficiency in PRDX1 suppresses autophagic flux in macrophages, leading to impaired lipophagic cholesterol hydrolysis, higher intracellular cholesterol, and reduced cholesterol efflux. 2-Cys PRDX mimics (ebselen, gliotoxin) rescue these defects, and Prdx1-deficient bone marrow transplantation into apoe-/- mice increases atherosclerotic plaque formation. Prdx1 knockout macrophages, lipophagic flux assays, cholesterol efflux assays, in vivo bone marrow transplant model, pharmacological rescue Autophagy High 28605287
2013 AMPK activation stabilises the interaction between c-Abl and PRDX1, preventing oxidative stress-induced dissociation of the complex. Knockdown of PRDX1 increases phosphorylation of both caveolin-1 and c-Abl and abolishes the inhibitory effect of AMPK on caveolin-1 phosphorylation and albumin endocytosis in endothelial cells. Co-immunoprecipitation, siRNA knockdown, pharmacological AMPK activation (AICAR), caveolin-1 phosphorylation and endocytosis assays The Journal of biological chemistry Medium 23723070
2021 PRDX1 oligomers bind both the Nedd8-conjugating enzyme UBE2F and CUL5, forming a tricomplex essential for CUL5 neddylation. This promotes NOXA ubiquitination and degradation, inhibiting apoptosis in colorectal cancer cells. ROS elevation drives PRDX1 oligomerization, linking oxidative stress to NOXA protein half-life. Co-immunoprecipitation, PRDX1 oligomerization assays, ubiquitination assays, NOXA protein half-life measurement, PRDX1 knockdown and oligomerization inhibition Cell death & disease Medium 33712558
2018 PRDX1 forms a heterodimer with p38α (MAPK14), stabilizing phospho-p38α in glioma cells, which amplifies HGF-mediated MET signaling and stimulates actin cytoskeleton dynamics to promote glioma cell migration and brain invasion in vivo. Biochemical interaction studies (Co-IP), in vitro and ex vivo migration assays, whole-brain high-resolution ultramicroscopy in mouse glioma models, survival analysis of PRDX1-KD mice International journal of cancer Medium 29582423
2021 PRDX1 interacts with ASK1 at elevated H2O2 concentrations in a manner that does not require a scaffolding protein, in contrast to the Prdx2:STAT3 relay. This interaction was demonstrated both in cellulo and by in vitro protein-protein interaction methods. Co-immunoprecipitation, in vitro pulldown, H2O2 dose-response experiments Antioxidants (Basel, Switzerland) Medium 34209102
2024 The lysine acetyltransferase MOF acetylates PRDX1 at lysine 197 (K197), preventing its hyperoxidation and maintaining peroxidase activity under inflammatory stress. LPS stimulation rapidly decreases PRDX1 K197ac in macrophages, elevating H2O2, augmenting ERK1/2 phosphorylation, stimulating glycolysis, and enhancing pro-inflammatory IL-6 production. Identification of K197 acetylation site, MOF KO/knockdown experiments, LPS stimulation, H2O2 measurement, ERK1/2 phosphorylation assays, metabolic flux analysis Cell reports High 39207899
2024 HJURP forms disulfide-linked intermediates with PRDX1 through Cys327 and Cys457 residues of HJURP, promoting PRDX1 redox cycling and inhibiting its hyperoxidation, thereby enhancing PRDX1 peroxidase activity and suppressing ROS and lipid peroxidation to confer ferroptosis resistance in prostate cancer cells. Co-immunoprecipitation, disulfide bond identification by mutagenesis, peroxidase activity assays, ROS and lipid peroxidation measurement, in vivo xenograft Redox biology Medium 39405980
2023 During the DNA damage response, PRDX1 translocates to the nucleus where it reduces DNA damage-induced nuclear ROS. Loss of PRDX1 lowers aspartate availability, impairing DNA damage-induced upregulation of de novo nucleotide synthesis, and causes accumulation of replication stress and DNA damage. Functional genomics (CRISPR screen), chromatin proteomics, metabolomics, nuclear fractionation/localization, etoposide treatment experiments Molecular systems biology High 37259925
2024 PRDX1 acts as a molecular chaperone by binding to CUL3, inhibiting CUL3-mediated NRF2 ubiquitination and stabilising NRF2, which drives GPX4 transcription. The Cys83 residue of PRDX1 is required for CUL3 binding and this activity is enhanced by conoidin A. IP-MS, Co-immunoprecipitation, Cys83Ser mutagenesis, ubiquitination assays, NRF2 nuclear translocation assays, PRDX1 KO mouse model (AOM/DSS) International journal of biological sciences High 39430237
2019 PRDX1 binds JNK1 and inhibits JNK kinase signaling, thereby suppressing the cancer-associated fibroblast (CAF)-like activated phenotype in mammary fibroblasts. Loss of PRDX1 results in development of a CAF-like phenotype that is reversed by JNK inhibition. Co-immunoprecipitation of PRDX1 with JNK1, PRDX1 knockout mouse fibroblasts, JNK inhibitor (SP600125) rescue, migration/invasion assays BMC cancer Medium 31419957
2023 The lncRNA LncFASA directly binds the Ahpc-TSA domain of PRDX1 and drives liquid-liquid phase separation of PRDX1 into droplets, inhibiting its peroxidase activity. This disrupts ROS homeostasis and promotes ferroptosis through the SLC7A11-GPX4 axis in triple-negative breast cancer cells. RNA-protein binding assays, phase separation assays, peroxidase activity assays, SLC7A11/GPX4 pathway analysis, xenograft tumor model Science China. Life sciences Medium 37955780
2020 Prdx1 regulates the HEF1-Aurora A-HDAC6 signaling axis to promote primary cilia disassembly in esophageal squamous cell carcinoma cells; silencing Prdx1 restores primary cilia formation and reduces tumor formation in vivo. Prdx1 siRNA knockdown, gene chip analysis, cilia formation assays, HEF1/Aurora A/HDAC6 pathway measurement, in vivo xenograft BMC cancer Medium 32357862
2020 Prdx1 functions as an RNA-binding protein that stabilises inflammation- and apoptosis-related mRNAs after intracerebral haemorrhage, as identified by RIP-seq. Prdx1 overexpression reduces inflammation and apoptosis in ICH models. RNA immunoprecipitation combined with high-throughput sequencing (RIP-seq), AAV-mediated overexpression in rat ICH model Frontiers in neuroscience Medium 32210752
2024 Hspb1 directly interacts with Anxa2 to reduce its aggregation and phosphorylation, which in turn allows Anxa2 to interact with Prdx1 and maintain its antioxidative activity by decreasing Thr-90 phosphorylation of Prdx1; overexpression of Hspb1 had no protective effect in acinar-specific Prdx1 knockout mice, establishing the Hspb1/Anxa2/Prdx1 axis. Co-immunoprecipitation, Prdx1 acinar cell-specific KO mice, AAV8-Hspb1 overexpression, Thr-90 phosphorylation assays, genetic epistasis International journal of biological sciences Medium 38481805
2023 PRDX1 Cys52Ser (peroxidase-dead) variant mice show impaired global PRDX peroxidase activity and are protected from diet-induced NASH and liver fibrosis, with suppressed NF-κB and STAT1 pro-inflammatory signalling, demonstrating that peroxidatic Cys52 is required for PRDX1's pro-inflammatory activity in vivo. Knock-in mouse model (Cys52Ser), Trx-TrxR-NADPH coupled activity assay, western diet/MCD diet feeding, RNA-seq, NF-κB and STAT1 pathway analysis Molecular metabolism High 37562742
2023 Kinetic modelling established that the PRDX1 dimer-to-decamer transition (association rate 0.050 µM⁻⁴·s⁻¹, dissociation rate 0.055 s⁻¹) has an inhibition-like effect on peroxidase activity, as the decameric form has ~100-fold lower activity than the dimer. Incorporating this transition into an in vivo PRDX model reconciled experimental and simulated oxidation-state responses. Isothermal titration calorimetry data fitting, kinetic modelling, HRP competition and NADPH-oxidation assays, in vivo PRDX2 erythrocyte model simulation Antioxidants (Basel, Switzerland) Medium 37760010
2024 ZNF207 promotes lactylation of PRDX1 at lysine 67, which enhances PRDX1 nuclear translocation and activation of NRF2, creating a ferroptosis-resistant environment in HCC cells and driving regorafenib resistance. CRISPR/Cas9 screening, lactylation site identification (K67), nuclear translocation assays, NRF2 activity assays, RGF sensitivity rescue experiments Drug resistance updates Medium 40680452
2021 IRAK1 binds PRDX1 and prevents its ubiquitination and degradation mediated by the E3 ubiquitin ligase HECTD3 (which interacts with PRDX1 via its DOC and HECT domains). Overexpression of PRDX1 reverses the radiosensitisation effect of IRAK1 depletion by diminishing autophagic cell death in glioma. Co-IP, LC-MS/MS, GST pulldown, ubiquitination assays, domain-mapping mutagenesis of HECTD3, PRDX1 overexpression rescue Cell death & disease Medium 37031183
2024 PRDX1 directly interacts with the actin-binding protein Cofilin, inhibiting phosphorylation of Cofilin at Ser3, thereby accelerating actin depolymerisation and turnover and promoting OSCC cell migration and invasion. Co-immunoprecipitation, Cofilin Ser3 phosphorylation assays, actin dynamics assays, in vitro migration/invasion, nude mouse tongue cancer model International journal of cancer Medium 38738971
2025 A co-crystal structure of PRDX1 with the celastrol derivative LC-PDin20 revealed the binding mode of covalent inhibitors with PRDX1, and active cysteine residues were confirmed as the covalent binding sites, enabling structure-based design of selective PRDX1 inhibitors. Co-crystal structure determination (X-ray crystallography), molecular docking, in vitro PRDX1 enzyme inhibition assay (IC50 determination) Journal of medicinal chemistry High 40546088
2024 Recombinant PRDX1 protein dose-dependently inhibits ROS production and osteoclast differentiation from bone marrow macrophages, and mechanistically attenuates NFATc1 activation as well as expression of C-Fos, V-ATPase-d2, Cathepsin K, and Integrin αV downstream of RANKL signalling. Recombinant protein treatment, ROS measurement, osteoclast differentiation assay, NFATc1 and downstream marker expression analysis Journal of cellular physiology Medium 39263840
2023 BACH1 directly binds the promoter region of PRDX1 and inhibits its transcription; remifentanil treatment suppresses BACH1 and upregulates PRDX1, thereby reducing oxidative stress injury in hepatic ischaemia-reperfusion. Chromatin immunoprecipitation (ChIP), dual luciferase reporter assay, BACH1 overexpression and PRDX1 silencing epistasis Clinics and research in hepatology and gastroenterology Medium 39025461
2025 NFAT5 directly binds the PRDX1 promoter to drive its transcription, which enhances mitochondrial function in cervical cancer cells. METTL3 promotes NFAT5 mRNA stability via IGF2BP3-mediated m6A modification, placing PRDX1 downstream of an m6A-NFAT5 transcriptional regulatory axis. ChIP assay, dual-luciferase reporter assay, MeRIP assay, NFAT5 knockdown/overexpression with PRDX1 rescue Journal of biochemical and molecular toxicology Medium 39925026

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells. Genome biology 1141 30890159
2003 Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression. Nature 646 12891360
2018 Inhibition of TRAF6 ubiquitin-ligase activity by PRDX1 leads to inhibition of NFKB activation and autophagy activation. Autophagy 183 29929436
2021 PRDX1 activates autophagy via the PTEN-AKT signaling pathway to protect against cisplatin-induced spiral ganglion neuron damage. Autophagy 182 33749526
2009 The antioxidant enzyme Prdx1 controls neuronal differentiation by thiol-redox-dependent activation of GDE2. Cell 93 19766572
2008 Proteomic expression analysis of surgical human colorectal cancer tissues: up-regulation of PSB7, PRDX1, and SRP9 and hypoxic adaptation in cancer. Journal of proteome research 89 18549262
2002 HIV-1 antiviral activity of recombinant natural killer cell enhancing factors, NKEF-A and NKEF-B, members of the peroxiredoxin family. The Journal of biological chemistry 81 12421812
2017 Prdx1 (peroxiredoxin 1) deficiency reduces cholesterol efflux via impaired macrophage lipophagic flux. Autophagy 76 28605287
2013 Role for Prdx1 as a specific sensor in redox-regulated senescence in breast cancer. Oncogene 74 23334324
2018 Prdx1 alleviates cardiomyocyte apoptosis through ROS-activated MAPK pathway during myocardial ischemia/reperfusion injury. International journal of biological macromolecules 73 29410271
2018 PRDX1 and MTH1 cooperate to prevent ROS-mediated inhibition of telomerase. Genes & development 64 29773556
2021 PRDX-1 Supports the Survival and Antitumor Activity of Primary and CAR-Modified NK Cells under Oxidative Stress. Cancer immunology research 60 34853030
2018 FGF1 improves functional recovery through inducing PRDX1 to regulate autophagy and anti-ROS after spinal cord injury. Journal of cellular and molecular medicine 56 29512938
2005 Histone deacetylase inhibitor FK228 activates tumor suppressor Prdx1 with apoptosis induction in esophageal cancer cells. Clinical cancer research : an official journal of the American Association for Cancer Research 54 16278420
2023 LncFASA promotes cancer ferroptosis via modulating PRDX1 phase separation. Science China. Life sciences 45 37955780
2006 Cloning and characterisation of two natural killer enhancing factor genes (NKEF-A and NKEF-B) in pufferfish, Tetraodon nigroviridis. Fish & shellfish immunology 43 16690325
2021 Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1. Acta pharmacologica Sinica 42 33833406
2022 18beta-glycyrrhetinic acid induces ROS-mediated apoptosis to ameliorate hepatic fibrosis by targeting PRDX1/2 in activated HSCs. Journal of pharmaceutical analysis 41 36105163
2018 Differential parameters between cytosolic 2-Cys peroxiredoxins, PRDX1 and PRDX2. Protein science : a publication of the Protein Society 41 30284335
2023 Dual role of PRDX1 in redox-regulation and tumorigenesis: Past and future. Free radical biology & medicine 40 37977211
2021 Cullin-5 neddylation-mediated NOXA degradation is enhanced by PRDX1 oligomers in colorectal cancer. Cell death & disease 38 33712558
2013 AMP-dependent kinase inhibits oxidative stress-induced caveolin-1 phosphorylation and endocytosis by suppressing the dissociation between c-Abl and Prdx1 proteins in endothelial cells. The Journal of biological chemistry 38 23723070
2013 PAG--a multipurpose transmembrane adaptor protein. Oncogene 37 24213579
2011 Downregulation of PRDX1 by promoter hypermethylation is frequent in 1p/19q-deleted oligodendroglial tumours and increases radio- and chemosensitivity of Hs683 glioma cells in vitro. Oncogene 37 22158042
1994 Organization and chromosomal assignment of two human PAG gene loci: PAGA encoding a functional gene and PAGB a processed pseudogene. Genomics 37 8188254
2019 PRDX1 enhances cerebral ischemia-reperfusion injury through activation of TLR4-regulated inflammation and apoptosis. Biochemical and biophysical research communications 35 31526567
2016 Identification and verification of PRDX1 as an inflammation marker for colorectal cancer progression. American journal of translational research 35 27158373
2022 HBXIP induces anoikis resistance by forming a reciprocal feedback loop with Nrf2 to maintain redox homeostasis and stabilize Prdx1 in breast cancer. NPJ breast cancer 33 35027562
2020 PRDX1 Counteracts Catastrophic Telomeric Cleavage Events That Are Triggered by DNA Repair Activities Post Oxidative Damage. Cell reports 33 33147465
2024 Hspb1 protects against severe acute pancreatitis by attenuating apoptosis and ferroptosis via interacting with Anxa2 to restore the antioxidative activity of Prdx1. International journal of biological sciences 32 38481805
2023 Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells. Cell death & disease 30 37031183
2023 A metabolic map of the DNA damage response identifies PRDX1 in the control of nuclear ROS scavenging and aspartate availability. Molecular systems biology 30 37259925
2014 Proteomic analysis of lung tissue in a rat acute lung injury model: identification of PRDX1 as a promoter of inflammation. Mediators of inflammation 30 25024510
2023 PRDX1 negatively regulates bleomycin-induced pulmonary fibrosis via inhibiting the epithelial-mesenchymal transition and lung fibroblast proliferation in vitro and in vivo. Cellular & molecular biology letters 29 37268886
2013 PRDX1 and PRDX6 are repressed in papillary thyroid carcinomas via BRAF V600E-dependent and -independent mechanisms. International journal of oncology 28 24316730
2023 The super-enhancer-driven lncRNA LINC00880 acts as a scaffold between CDK1 and PRDX1 to sustain the malignance of lung adenocarcinoma. Cell death & disease 26 37620336
2015 PRDX1 is involved in palmitate induced insulin resistance via regulating the activity of p38MAPK in HepG2 cells. Biochemical and biophysical research communications 26 26301632
2012 Proteomics in HepG2 hepatocarcinoma cells with stably silenced expression of PRDX1. Journal of proteomics 26 23277276
2012 Prdx1 deficiency in mice promotes tissue specific loss of heterozygosity mediated by deficiency in DNA repair and increased oxidative stress. Mutation research 25 22583657
2017 Prdx1-encoded peroxiredoxin is important for vascular development in zebrafish. FEBS letters 24 28231391
2020 Prdx1 Reduces Intracerebral Hemorrhage-Induced Brain Injury via Targeting Inflammation- and Apoptosis-Related mRNA Stability. Frontiers in neuroscience 23 32210752
2005 Transformation of an edible crop with the pagA gene of Bacillus anthracis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23 16030177
2024 PRDX1 inhibits ferroptosis by binding to Cullin-3 as a molecular chaperone in colorectal cancer. International journal of biological sciences 22 39430237
2013 Regulation of PRDX1 peroxidase activity by Pin1. Cell cycle (Georgetown, Tex.) 22 23421996
2020 PRDX1 stimulates non-small-cell lung carcinoma to proliferate via the Wnt/β-Catenin signaling. Panminerva medica 20 32881473
2019 Bacillus anthracis Virulence Regulator AtxA Binds Specifically to the pagA Promoter Region. Journal of bacteriology 20 31570528
2018 Global analysis of erythroid cells redox status reveals the involvement of Prdx1 and Prdx2 in the severity of beta thalassemia. PloS one 20 30521599
2025 Discovery of Natural Resorcylic Acid Lactones as Novel Potent Copper Ionophores Covalently Targeting PRDX1 to Induce Cuproptosis for Triple-Negative Breast Cancer Therapy. ACS central science 19 40028362
2019 The peroxidase PRDX1 inhibits the activated phenotype in mammary fibroblasts through regulating c-Jun N-terminal kinases. BMC cancer 19 31419957
2024 HJURP inhibits sensitivity to ferroptosis inducers in prostate cancer cells by enhancing the peroxidase activity of PRDX1. Redox biology 18 39405980
2015 Identification of RAB2A and PRDX1 as the potential biomarkers for oral squamous cell carcinoma using mass spectrometry-based comparative proteomic approach. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 18 26159854
2022 PRDX1 Influences The Occurrence and Progression of Liver Cancer by Inhibiting Mitochondrial Apoptosis Pathway. Cell journal 17 36377215
2020 Prdx1 promotes the loss of primary cilia in esophageal squamous cell carcinoma. BMC cancer 17 32357862
2020 PRDX1 is a Tumor Suppressor for Nasopharyngeal Carcinoma by Inhibiting PI3K/AKT/TRAF1 Signaling. OncoTargets and therapy 17 32982301
2024 Matairesinol blunts adverse cardiac remodeling and heart failure induced by pressure overload by regulating Prdx1 and PI3K/AKT/FOXO1 signaling. Phytomedicine : international journal of phytotherapy and phytopharmacology 16 39306883
2019 Decreased expression of microRNA-510 in intestinal tissue contributes to post-infectious irritable bowel syndrome via targeting PRDX1. American journal of translational research 15 31934286
2018 BMP signaling-driven osteogenesis is critically dependent on Prdx-1 expression-mediated maintenance of chondrocyte prehypetrophy. Free radical biology & medicine 15 29452246
2025 ZNF207-driven PRDX1 lactylation and NRF2 activation in regorafenib resistance and ferroptosis evasion. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 14 40680452
2023 Chronic exposure to low-dose deltamethrin can lead to colon tissue injury through PRDX1 inactivation-induced mitochondrial oxidative stress injury and gut microbial dysbiosis. Ecotoxicology and environmental safety 14 37714033
2021 PRDX1 gene-related epi-cblC disease is a common type of inborn error of cobalamin metabolism with mono- or bi-allelic MMACHC epimutations. Clinical epigenetics 14 34215320
2017 l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. Theriogenology 14 28965027
2024 Furanodienone induces apoptosis via regulating the PRDX1/MAPKs/p53/caspases signaling axis through NOX4-derived mitochondrial ROS in colorectal cancer cells. Biochemical pharmacology 13 39079582
2023 PRDX1 Cys52Ser variant alleviates nonalcoholic steatohepatitis by reducing inflammation in mice. Molecular metabolism 12 37562742
2022 miR-375 suppresses the growth and metastasis of esophageal squamous cell carcinoma by targeting PRDX1. Journal of gastrointestinal oncology 12 36388649
2021 Prdx1 Interacts with ASK1 upon Exposure to H2O2 and Independently of a Scaffolding Protein. Antioxidants (Basel, Switzerland) 12 34209102
2020 Inhibition of the antioxidant enzyme PRDX1 activity promotes MPP+-induced death in differentiated SH-SY5Y cells and may impair its colocalization with eEF1A2. Life sciences 12 32781074
2018 A PRDX1-p38α heterodimer amplifies MET-driven invasion of IDH-wildtype and IDH-mutant gliomas. International journal of cancer 12 29582423
2018 Peroxiredoxin 1 (PRDX1) Suppresses Progressions and Metastasis of Osteosarcoma and Fibrosarcoma of Bone. Medical science monitor : international medical journal of experimental and clinical research 10 29908016
2012 Identification of proteins containing redox-sensitive thiols after PRDX1, PRDX3 and GCLC silencing and/or glucose oxidase treatment in Hepa 1-6 cells. Journal of proteomics 10 22975676
2024 MOF-mediated PRDX1 acetylation regulates inflammatory macrophage activation. Cell reports 9 39207899
2023 TDP43/HDAC6/Prdx1 signaling pathway participated in the cognitive impairment of obstructive sleep apnea via regulating inflammation and oxidative stress. International immunopharmacology 9 38104368
2021 PRDX1 is essential for the viability and maintenance of reactive oxygen species in chicken DT40. Genes and environment : the official journal of the Japanese Environmental Mutagen Society 9 34353368
2025 Artesunate induces ferroptosis in diffuse large B-cell lymphoma cells by targeting PRDX1 and PRDX2. Cell death & disease 8 40645965
2011 [Effects of yiqi chutan recipe on tumor growth, survival time and expressions of PRDX-1 and PRDX-6 in Lewis lung carcinoma model mice with pi-deficiency syndrome]. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine 8 21434354
2024 The interaction of PRDX1 with Cofilin promotes oral squamous cell carcinoma metastasis. International journal of cancer 7 38738971
2023 Hepatocytes-derived Prdx1 regulates macrophage phenotypes via TLR4 activation in acute liver injury. International immunopharmacology 6 38159556
2005 Rare allelic imbalances, but no mutations of the PRDX1 gene in human hepatocellular carcinomas. Journal of clinical pathology 6 16254121
2025 PRDX1 knockdown promotes erastin-induced ferroptosis and impedes diffuse large B-cell lymphoma development by inhibiting the MAPK/ERK pathway. BMC cancer 5 40307771
2024 PRDX1 Interfering Peptide Disrupts Amino Acids 70-90 of PRDX1 to Inhibit the TLR4/NF-κB Signaling Pathway and Attenuate Neuroinflammation and Ischemic Brain Injury. Molecular neurobiology 5 38780721
2024 PRDX1 exerts a photoprotection effect by inhibiting oxidative stress and regulating MAPK signaling on retinal pigment epithelium. BMC ophthalmology 5 38844903
2024 Remifentanil represses oxidative stress to relieve hepatic ischemia/reperfusion injury via regulating BACH1/PRDX1 axis. Clinics and research in hepatology and gastroenterology 5 39025461
2023 Altered Regulation of the Glucose Transporter GLUT3 in PRDX1 Null Cells Caused Hypersensitivity to Arsenite. Cells 5 38067110
2021 Fish natural killer enhancing factor-A (NKEF-A) enhance cytotoxicity of nonspecific cytotoxic cells against bacterial infection. Molecular immunology 5 33744653
2025 Rapid Discovery of Celastrol Derivatives as Potent and Selective PRDX1 Inhibitors via Microplate-Based Parallel Compound Library and In Situ Screening. Journal of medicinal chemistry 4 40546088
2024 Antioxidant enzyme Prdx1 inhibits osteoclastogenesis via suppressing ROS and NFATc1 signaling pathways. Journal of cellular physiology 4 39263840
2023 MicroRNA-146a Improved Acute Lung Injury Induced by hepatic Ischemia-reperfusion Injury by Inhibiting PRDX1. Dose-response : a publication of International Hormesis Society 4 37063344
2023 Effects of Date Palm Pollen Supplementations on The Expression of PRDX1 and PRDX6 Genes in Infertile Men: A Controlled Clinical Trial. International journal of fertility & sterility 4 37183847
2025 Panoptosis Targets the Mechanism of PRDX1 Involvement in Acute Myocardial Infarction via ZBP1. Journal of cellular biochemistry 3 40114419
2025 Tumor-associated bacteria activate PRDX1-driven glycolysis to promote immune evasion and PD-1 antibody resistance in hepatocellular carcinoma. Frontiers in microbiology 3 40693141
2025 Glaucocalyxin A induces autophagy-mediated ferroptosis by targeting PRDX1 and TXNRD1 proteins in non-small cell lung cancer. Phytomedicine : international journal of phytotherapy and phytopharmacology 3 41016297
2024 Molecular characterization, cytoprotective, DNA protective, and immunological assessment of peroxiredoxin-1 (Prdx1) from yellowtail clownfish (Amphiprion clarkii). Developmental and comparative immunology 3 38574831
2023 Modelling the Decamerisation Cycle of PRDX1 and the Inhibition-like Effect on Its Peroxidase Activity. Antioxidants (Basel, Switzerland) 3 37760010
2022 A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system. STAR protocols 3 35284843
2020 [Effect and mechanism of PRDX1 in epithelial mesenchymal transformationin of gastric cancer cells]. Zhonghua zhong liu za zhi [Chinese journal of oncology] 3 33256302
2025 l-Glutamic Acid Alleviates Mastitis in Dairy Cows by Targeting the Sirt5/Nrf2/Prdx1 Signaling Axis and Mitochondrial Function. Journal of agricultural and food chemistry 2 39895052
2025 METTL3-mediated NFAT5 Upregulation Promotes Cervical Cancer Progression Through Enhancing the Mitochondrial Function by Transcriptional Regulation of PRDX1. Journal of biochemical and molecular toxicology 2 39925026
2025 PRDX1 affects acrylamide-induced neural damage through the PTEN/AKT signaling pathway. Neurotoxicology 2 40189060
2025 Targeting PRDX1 impairs acute myeloid leukemic blasts and stem cells by disrupting redox homeostasis. Cell death & disease 2 40825764
2023 6-Shogaol prevents benzo (A) pyrene-exposed lung carcinogenesis via modulating PRDX1-associated oxidative stress, inflammation, and proliferation in mouse models. Environmental toxicology 2 37638803
2013 Genomic organization, single nucleotide polymorphism and functional characterization of natural killer enhancing factor (NKEF-A) in Miichthys miiuy. Molecular biology reports 2 24352703