| 2019 |
Mechanical forces from cardiac contractility regulate cardiomyocyte myofilament maturation through the VCL-SSH1-CFL axis: vinculin (VCL) recruits the phosphatase SSH1 and its effector cofilin (CFL) to regulate F-actin rearrangement and promote myofilament maturation in zebrafish cardiomyocytes. |
Interactome profiling (contracting vs. non-contracting cardiomyocytes), Co-IP, genetic loss-of-function, live imaging, epistasis analysis |
Developmental cell |
High |
31495694
|
| 2017 |
LIMK1 directly phosphorylates and inactivates CFL1; inhibition of LIMK1 reduces CFL1 expression and phosphorylation, thereby impairing CFL1-dependent F-actin/G-actin remodeling and suppressing breast cancer cell migration and invasion. |
Western blot for pCFL1/CFL1 following LIMK1 knockdown, migration/invasion assays, miRNA mimic transfection in TNBC cells |
Oncotarget |
Medium |
29156719
|
| 2020 |
SSH1, the canonical CFL (cofilin) phosphatase, dephosphorylates phospho-Ser403-SQSTM1/p62, impairing autophagic cargo clearance; this action is separable from SSH1-mediated CFL activation, demonstrating that SSH1 has a CFL-independent substrate. |
RNAi knockdown, overexpression with defined mutant constructs, genetically encoded fluorescent reporters, cell lines and primary neurons |
Autophagy |
High |
33044112
|
| 2021 |
Hhex enhances the interaction of RHOGDIA with RHOA/CDC42, maintaining them in inactive forms, which blocks the RHOA/CDC42–LIMK1–pCFL1 signaling cascade, preserving CFL1 F-actin-severing activity and suppressing filopodium/lamellipodium formation and cell migration in lung cancer cells. |
Western blot, co-immunoprecipitation, wound-healing scratch assay, laser confocal imaging, overexpression/knockdown in NSCLC cells |
Cell communication and signaling : CCS |
Medium |
34321041
|
| 2023 |
HUNK kinase directly phosphorylates GEF-H1 at serine 645, which activates RhoA and triggers a cascade of LIMK-1/CFL-1 phosphorylation that stabilizes F-actin and inhibits EMT and metastasis in colorectal cancer cells. |
In vitro kinase assay (direct phosphorylation), Western blot for phospho-CFL-1, overexpression/knockdown, metastasis assays |
Cell death & disease |
Medium |
37193711
|
| 2023 |
CFL1 promotes phosphoglycerate dehydrogenase (PHGDH) transcription to enhance serine synthesis and metabolism, increasing antioxidant production that scavenges sorafenib-induced ROS, thereby impairing sorafenib sensitivity in hepatocellular carcinoma. |
Transcriptome sequencing of sorafenib-sensitive vs. insensitive HCC patients, siRNA knockdown, metabolic assays, nanoparticle co-delivery experiments in vivo |
Advanced science (Weinheim, Baden-Wurttemberg, Germany) |
Medium |
37203277
|
| 2022 |
Estradiol (E2) dose-dependently increases total CFL1 and phosphorylated CFL1 (pCFL1) expression in PBMCs and endocervical mucosa, and this induction is abrogated by LIMK1/2 inhibitor LIMKi3; CFL1 knockdown partially restores HIV-1 infection suppressed by E2, linking E2-mediated CFL1 phosphorylation to inhibition of HIV-1BaL infection. |
LIMK inhibitor treatment, CFL1 siRNA knockdown, protein expression by Western blot, HIV-1 infectivity assay in PBMCs and endocervical explants |
Scientific reports |
Medium |
35418661
|
| 2024 |
CFL1 overexpression in pSS bone marrow MSCs restores their migratory capacity by upregulating CCR1 expression; pharmacological inhibition of CCR1 suppresses the CFL1-induced migration and proliferation, placing CFL1 upstream of the CCL5/CCR1 axis. |
Lentivirus-mediated CFL1 overexpression, RNA-seq, Transwell migration assay, wound healing assay, CCR1 inhibitor rescue experiment, NOD mouse model |
International immunopharmacology |
Medium |
38183912
|
| 2024 |
Expression of a non-functional form of Cfl1 (nf-Cfl1) in connective tissue mast cells (CTMCs) via Mcpt5-Cre results in complete absence of CTMCs, demonstrating that Cfl1-dependent actin depolymerization is essential for CTMC formation; CTMCs lacking Cfl1 activity show impaired systemic anaphylaxis. |
Conditional knock-in of non-functional Cfl1 in CTMCs (Mcpt5-Cre-nf-Cfl1fl/fl), flow cytometry, passive systemic anaphylaxis model |
Frontiers in immunology |
High |
41684538
|
| 2025 |
Overexpression of Cfl1 in the polymorphic layer of the hippocampal dentate gyrus (PoDG) inhibits ML-DG synapses, increases motivation to seek alcohol, impairs extinction of alcohol-seeking, and decreases reward consumption during relapse; reducing Cfl1 levels has opposite effects, establishing hippocampal Cfl1 as a regulator of synaptic transmission and AUD-relevant behavior. |
Local viral overexpression/knockdown of Cfl in hippocampal DG subregions, in vivo two-photon imaging of synaptic activity, automated IntelliCage behavioral testing, RNA sequencing |
Molecular psychiatry |
Medium |
40931167
|
| 2026 |
c-Myc transcriptionally activates CFL1 by binding E-boxes in the CFL1 promoter (confirmed by ChIP-qPCR); cofilin-1 upregulation mediates c-Myc-induced oncogene-induced senescence (OIS) and F-actin/nuclear G-actin accumulation in lung cancer cells; knockdown of cofilin-1 suppresses cMIS, and a physical interaction between c-Myc and cofilin-1 was detected by co-immunoprecipitation. |
ChIP-qPCR, cofilin-1 knockdown, c-Myc overexpression/truncation mutants, Co-IP, F-actin/G-actin assays, conditioned medium bystander assays |
Cell death discovery |
Medium |
41888102
|
| 2024 |
Conditional knockout of ADF and Cfl1 in microglia (ADF/Cfl1-KO) causes increased accumulation of stabilized F-actin and altered microtubule dynamics, reduces microglial fine process motility, and impairs migration toward laser-induced lesions in vivo; microglial ADF/Cfl1-deficiency also decreases learning and memory in mice. |
Conditional KO mouse model, in vivo two-photon imaging, F-actin staining, microtubule dynamics assays, behavioral memory tests |
bioRxivpreprint |
Medium |
bio_10.1101_2024.09.27.615114
|
| 1996 |
Human non-muscle cofilin (CFL1) was mapped to chromosome 11q13 by PCR in somatic cell hybrid panels and confirmed by FISH, while muscle-type cofilin (CFL2) was mapped to chromosome 14; CFL1 was identified as an actin-binding protein involved in actin-cofilin complex translocation from cytoplasm to nucleus. |
PCR in rodent-human somatic cell hybrids, FISH of genomic cosmid clones, radiation hybrid mapping |
Annals of human genetics |
High |
8800436
|
| 2024 |
CFL1 expression in nerves (rather than in tumor cells) within the head and neck squamous cell carcinoma (HNSCC) tumor microenvironment is associated with perineural invasion; HNSCC cells induce neuronal CFL1 expression, and conditional knockout of neuronal CFL1 impedes tumor-nerve interactions. |
Multiplex fluorescent immunohistochemistry, conditional neuronal CFL1 knockout, tumor-nerve co-culture models |
Molecular carcinogenesis |
Medium |
38353363
|
| 2024 |
Imatinib therapy in CML upregulates CFL1 expression and activity (cofilin, P-cofilin) and increases F-actin (including branched F-actin), indicating that imatinib affects F-actin remodeling in CML cells by regulating CFL1. |
Western blot for CFL1 and P-cofilin in CML patient samples and cell lines pre/post imatinib, F-actin staining |
Journal of Cancer |
Low |
38495482
|