Affinage

CTTN

Src substrate cortactin · UniProt Q14247

Length
550 aa
Mass
61.6 kDa
Annotated
2026-06-09
63 papers in source corpus 22 papers cited in narrative 22 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CTTN encodes cortactin (originally identified as the EMS1 gene product), an actin-binding scaffold that couples extracellular signaling to cytoskeletal remodeling and thereby drives cell migration, invasion, and anoikis resistance (PMID:8474448, PMID:9681820, PMID:17178864). The protein is organized into an F-actin-binding tandem-repeat domain, a proline-rich SH3-binding region, and a C-terminal SH3 domain, and is expressed as p80/p85 isoforms (PMID:9823470). It concentrates at cell-substratum contact sites and podosome-like structures and, together with N-WASP, supports cortactin-dependent actin assembly and clathrin-mediated endocytosis, with cortactin knockdown impairing transferrin uptake (PMID:8474448, PMID:18799755, PMID:21418910). Cortactin activity is set by phosphorylation: it is tyrosine-phosphorylated in proportion to src-family kinase activity, and undergoes a serine/threonine phosphorylation-dependent mobility shift downstream of MEK-ERK signaling that requires its helical-proline-rich region (PMID:10537323, PMID:8945620). Functionally, cortactin promotes tumor migration, invasion, and metastasis in part through PI3K/Akt and Wnt/β-catenin (via DKK-1 downregulation) signaling, and supports anoikis resistance through FAK and cytoskeletal integrity (PMID:17178864, PMID:36831511, PMID:41168318). Its transcription is induced by inflammatory and mechanical stimuli through NF-κB elements in the promoter and through a calreticulin-STAT3 axis (PMID:39162263, PMID:19684620), while its protein stability is controlled by E3 ubiquitin ligases RNF128, UCHL1, and CBLC that target it for K48-linked proteasomal degradation (PMID:30832692, PMID:32120844, PMID:36043996). A coding variant (Ser484Asn) that reduces Tyr486 phosphorylation and disrupts cortactin binding to nmMLCK impairs endothelial barrier recovery and fails to rescue lung vascular permeability in Cttn+/- mice, establishing cortactin's role in endothelial barrier function (PMID:35181549). Alternative splicing of CTTN, controlled by PTBP2 and RBMS1, generates isoforms that modulate invadopodia formation under matrix stiffness and PI3K/AKT-driven cardiac hypertrophy (PMID:38273817, PMID:41214391).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1993 Medium

    Establishing that the breast-cancer amplicon gene EMS1 encodes a src-substrate homolog localizing to cell-substratum contacts placed cortactin at the interface of oncogenic signaling and adhesion structures.

    Evidence Sequence comparison to a chicken v-src substrate plus immunocytochemistry in epithelial and carcinoma cells

    PMID:8474448

    Open questions at the time
    • Did not define the protein's domain architecture or biochemical activity
    • Localization correlative, no functional manipulation
  2. 1996 Medium

    Linking cortactin tyrosine phosphorylation to src-family kinase activity in breast cancer connected its modification state to upstream oncogenic kinases.

    Evidence Western blot and tyrosine phosphorylation analysis across breast cancer cell lines with src kinase activity assays

    PMID:8945620

    Open questions at the time
    • Correlative, no direct kinase-substrate demonstration
    • Phosphosites not mapped
    • Functional consequence of phosphorylation untested
  3. 1998 Medium

    Domain mapping and gain-of-function assays defined cortactin as an actin-binding scaffold whose overexpression directly increases motility and invasion independent of proliferation.

    Evidence Sequence/domain analysis with dual-isoform gene transfer, and stable overexpression in NIH3T3 with Boyden chamber assays

    PMID:9681820 PMID:9823470

    Open questions at the time
    • Mechanism linking domains to motility not resolved
    • No loss-of-function in this system
  4. 1999 High

    Identifying MEK as a necessary and sufficient intermediate for cortactin serine/threonine phosphorylation placed it downstream of a defined signaling cascade and localized the modification to the helical-proline-rich region.

    Evidence PD98059 inhibition, constitutively active MEK, in vitro kinase assay, deletion mutagenesis, and phosphopeptide mapping

    PMID:10537323

    Open questions at the time
    • Exact kinase beyond candidate ERKs not pinned down
    • Functional output of this phosphorylation not assayed here
  5. 2006 High

    Loss-of-function in esophageal carcinoma established that endogenous cortactin is required for migration, invasion, anoikis resistance, and metastasis, working through PI3K/Akt.

    Evidence siRNA knockdown with migration/anoikis assays, PI3K/Akt readouts, and in vivo xenograft metastasis

    PMID:17178864

    Open questions at the time
    • Direct mechanism of PI3K/Akt activation by cortactin not defined
    • Did not address transcriptional control
  6. 2009 High

    Defining a calreticulin-STAT3 transcriptional axis showed cortactin expression is directly driven by p53-independent STAT3 binding to its promoter, integrating it into a signaling-to-transcription circuit.

    Evidence siRNA, ChIP showing p-STAT3 binding to the CTTN promoter, JAK inhibition, and CTTN rescue of motility/anoikis

    PMID:19684620

    Open questions at the time
    • Other transcriptional inputs not assessed in this study
  7. 2011 Medium

    Demonstrating that cortactin knockdown reduces transferrin uptake established a non-migratory function in clathrin-mediated endocytosis requiring intact protein.

    Evidence siRNA and domain-deletion mutants with transferrin uptake assay in colon cancer cells

    PMID:21418910

    Open questions at the time
    • Specific endocytic step requiring cortactin not resolved
    • Partner machinery at coated vesicles not defined here
  8. 2019 Medium

    Identifying RNF128-mediated ubiquitination and a CTTN-DKK-1-Wnt/β-catenin link revealed both degradative control of cortactin and a downstream signaling output to stemness/EMT.

    Evidence Co-IP, ubiquitination assays, RNA-seq, and β-catenin/TCF inhibitor rescue in melanoma and HER2+ breast cancer

    PMID:30832692 PMID:36831511

    Open questions at the time
    • RNF128 ubiquitination sites on CTTN not mapped
    • Mechanism of DKK-1 downregulation by cortactin unclear
  9. 2019 Medium

    Showing HBx physically binds cortactin to upregulate CREB1 targets connected cortactin to a viral oncoprotein-driven proliferation program.

    Evidence Proteomics, Co-IP, confocal microscopy, and proliferation/migration assays in HCC cells

    PMID:31138777

    Open questions at the time
    • Direct vs scaffold-mediated interaction not distinguished
    • Mechanism of CREB1 activation downstream of the complex unclear
  10. 2020 Medium

    UCHL1 was shown to drive K48-linked cortactin degradation, with cortactin rescue restoring invasion, reinforcing the ubiquitin-proteasome control of cortactin levels.

    Evidence Co-IP, K48-linkage-specific ubiquitination assay, and CTTN rescue of migration/invasion in NPC cells

    PMID:32120844

    Open questions at the time
    • Single lab, sites of ubiquitination not mapped
    • Relationship to other ligases (RNF128, CBLC) not addressed
  11. 2022 High

    A coding SNP (Ser484Asn) that disrupts Tyr486 phosphorylation and nmMLCK binding causally linked cortactin to endothelial barrier function in vivo.

    Evidence Mutant transgene expression, phosphorylation and Co-IP assays, barrier biophysics, and WT-vs-mutant rescue in Cttn+/- ARDS mice

    PMID:35181549

    Open questions at the time
    • How Ser484 modification controls Tyr486 phosphorylation mechanistically not fully resolved
    • Generalizability beyond lung endothelium untested
  12. 2022 Medium

    CBLC was identified as a third E3 ligase degrading cortactin in the cytoplasm without affecting mRNA, consolidating proteasomal turnover as a major regulatory node.

    Evidence Co-IP, co-localization, proteasome pathway assay, and CTTN overexpression rescue in breast cancer cells

    PMID:36043996

    Open questions at the time
    • Functional redundancy/hierarchy among RNF128, UCHL1, CBLC unknown
    • Ubiquitination sites unmapped
  13. 2024 Medium

    Defining NF-κB-dependent promoter activation by inflammatory and mechanical stimuli, modulated by a promoter SNP and DNA methylation, established cortactin as a stress/inflammation-inducible gene.

    Evidence Luciferase reporter with site-directed mutagenesis of NF-κB sites/SNP, cyclic stretch, pharmacological modulators, and lung IHC

    PMID:39162263

    Open questions at the time
    • Relative contribution of HIF/NRF2 inputs not quantified
    • Link between transcriptional induction and barrier phenotypes not directly tested
  14. 2024 Medium

    Identifying PTBP2-controlled splicing of WT-CTTN under matrix stiffness introduced mechanosensitive isoform selection as a regulator of invadopodia formation.

    Evidence siRNA, overexpression, splicing analysis, and invadopodia/invasion assays across stiffness conditions in NPC cells

    PMID:38273817

    Open questions at the time
    • Functional differences among SV1/SV2 isoforms not fully defined
    • Single lab
  15. 2025 Medium

    RBMS1-driven generation of a CTTN-Δe11 isoform was shown to activate PI3K/AKT and cause sarcomere damage, extending splicing control of cortactin into cardiac hypertrophy.

    Evidence Splicing analysis, PI3K/AKT assays, and cardiac hypertrophy mouse model with pharmacological RBMS1 inhibition

    PMID:41214391

    Open questions at the time
    • How Δe11 isoform mechanistically engages PI3K/AKT unclear
    • Single lab
  16. 2025 Medium

    Linking cortactin to FAK-dependent cytoskeletal integrity in HNSCC and to Rab7+ late endosomal homeostasis whose loss triggers mTOR-p53 senescence broadened its role from migration into endosomal tethering and cell-fate control.

    Evidence siRNA with FAK Western blot and PDO assays; and Rab7/mTOR localization, p53 phosphorylation, senescence markers and CDX models (preprint)

    PMID:41168318 PMID:bio_10.1101_2025.03.26.645381

    Open questions at the time
    • Senescence work is a preprint, not peer-reviewed
    • Mechanism of cortactin-mediated late endosomal tethering not molecularly defined

Open questions

Synthesis pass · forward-looking unresolved questions
  • How cortactin integrates its diverse phosphorylation states, ubiquitin-driven turnover, isoform repertoire, and partner network into context-specific outputs (migration vs endocytosis vs barrier function vs senescence) remains unresolved.
  • No unified structural model integrating actin binding, phosphorylation, and partner recruitment
  • Hierarchy/redundancy among the three E3 ligases not established
  • Functional distinction among splice isoforms incompletely mapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005856 cytoskeleton 3 GO:0005829 cytosol 2 GO:0005768 endosome 1 GO:0005886 plasma membrane 1
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-1643685 Disease 3 R-HSA-5653656 Vesicle-mediated transport 2
Partners
CBLCHBXLAD1MYLKRNF128UCHL1WASLYWHAG

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 The EMS1 gene product (human cortactin, 80/85 kDa) is homologous (85% identity) to a chicken v-src substrate. In normal epithelial cells, the protein localizes mainly to the cytoplasm with minor presence in leading lamellae; in carcinoma cells overexpressing EMS1, it accumulates at podosome-like adherens junctions associated with cell-substratum contact sites, but not intercellular adherens junctions. Amino acid sequence comparison, immunocytochemistry Molecular and cellular biology Medium 8474448
1998 The EMS1/cortactin protein contains: (i) a filamentous actin-binding tandem repeat domain, (ii) a proline-rich SH3-binding region, and (iii) a SH3 domain. Both human p80 and p85 isoforms are encoded by the EMS1 cDNA. Expression is restricted to non-lymphoid tumor cell lines. Gene transfer experiments, western blot, sequence analysis Cell adhesion and communication Medium 9823470
1998 Stable overexpression of EMS1/cortactin in NIH3T3 fibroblasts increases cell motility and invasiveness in modified Boyden chamber assays without altering proliferation or anchorage independence, indicating cortactin promotes cell migration and invasion. Stable transfection, modified Boyden chamber motility/invasion assay, proliferation assay Oncogene Medium 9681820
1999 EGF treatment or cell detachment induces an 80–85 kDa mobility shift of EMS1 (cortactin) correlated with increased serine/threonine phosphorylation. This shift is blocked by the MEK inhibitor PD98059 and is induced by constitutively active MEK, establishing MEK as a necessary and sufficient intermediate in EMS1 phosphorylation. The helical-proline-rich region is required for this phosphorylation. ERKs are candidate kinases for this region in vitro, but other MEK-regulated enzymes also participate. Pharmacological inhibition (PD98059), constitutively active MEK expression, in vitro kinase assay, deletion mutagenesis, tryptic phosphopeptide mapping Cancer research High 10537323
1996 EMS1 protein is tyrosine phosphorylated in breast cancer cell lines; degree of tyrosine phosphorylation correlates with src-family kinase activity (c-fyn and c-yes expression). Gene amplification is the predominant mechanism of EMS1 overexpression in breast cancer cell lines. Western blot, tyrosine phosphorylation analysis, src kinase activity assay International journal of cancer Medium 8945620
2006 siRNA-mediated silencing of CTTN in esophageal squamous cell carcinoma cells reduces cell migration, invasiveness, and anoikis resistance. The protective role of CTTN in anoikis resistance correlates with activation of the PI3K/Akt pathway. In vivo, CTTN knockdown decreased tumor growth and lung metastasis. siRNA knockdown, cell migration assay, anoikis assay, PI3K/Akt signaling analysis, in vivo xenograft Cancer research High 17178864
2009 Calreticulin (CRT) regulates CTTN expression via STAT3: CRT knockdown reduces p-STAT3, and chromatin immunoprecipitation shows direct binding of p-STAT3 to STAT3-binding sequences in the CTTN promoter. CRT-enhanced anoikis resistance and motility operate through a CRT–STAT3–CTTN–PI3K–Akt pathway; CTTN restoration in CRT-depleted cells rescues motility and anoikis resistance. siRNA knockdown, ChIP assay, JAK inhibitor (AG490), Western blot, cell migration/invasion/anoikis assays, CTTN rescue experiment Oncogene High 19684620
2019 RNF128 (an E3 ubiquitin ligase) ubiquitinates and promotes degradation of CTTN (cortactin), activating Wnt/β-catenin signaling and CD44/c-Myc transcription. RNF128 downregulation leads to CTTN accumulation, inducing EMT and stemness in melanoma. Co-IP, ubiquitination assay, siRNA interference, functional EMT/stemness assays Journal of hematology & oncology Medium 30832692
2020 UCHL1 interacts with CTTN and promotes K48-linked ubiquitination of CTTN, leading to its proteasomal degradation. CTTN rescue in UCHL1-overexpressing NPC cells restores migration and invasion, placing CTTN as a functional downstream target of UCHL1. Co-IP, ubiquitination assay (K48-linkage specific), in vitro/in vivo migration and invasion assays, CTTN rescue experiment Cells Medium 32120844
2022 The CTTN coding SNP Ser484Asn (rs56162978) reduces Tyr486 cortactin phosphorylation, inhibits binding of cortactin to nmMLCK, delays endothelial cell barrier recovery after thrombin-induced permeability, and attenuates lamellipodia dynamics. In Cttn+/- heterozygous mice, increased lung vascular permeability was rescued by WT CTTN transgene but not by S484N transgene. Transgene expression in human lung ECs, phosphorylation assay, Co-IP (cortactin–nmMLCK binding), biophysical barrier assay, liposome-mediated transgene delivery in vivo in ARDS mouse model Translational research High 35181549
2022 CBLC (an E3 ubiquitin ligase) interacts with CTTN in the cytoplasm and promotes CTTN degradation through the ubiquitin-proteasome pathway without affecting CTTN mRNA levels. CBLC-mediated inhibition of breast cancer cell proliferation, migration, and invasion is partially reversed by CTTN overexpression. Co-IP, immunofluorescence co-localization, ubiquitin-proteasome pathway assay, rescue experiment with CTTN overexpression Journal of receptor and signal transduction research Medium 36043996
2008 Cortactin (CTTN) and N-WASP, two regulators of actin network assembly, are concentrated at tubulobulbar complexes in the rat testis seminiferous epithelium alongside clathrin, supporting that these podosome-like structures involve cortactin-dependent actin assembly and clathrin-mediated endocytosis. Immunofluorescence microscopy, immunoelectron microscopy on rat testis Biology of reproduction Low 18799755
2011 siRNA silencing of cortactin (CTTN) in colon cancer cells reduces transferrin uptake (endocytosis). Intact cortactin protein and sufficient expression level are required for optimal clathrin-coated vesicle-mediated endocytosis in cancer cells. siRNA knockdown, domain-deletion mutant transfection, transferrin uptake assay, immunohistochemistry Zhonghua yi xue za zhi Medium 21418910
2019 CTTN overexpression downregulates DKK-1, a Wnt antagonist, thereby activating Wnt/β-catenin signaling. A β-catenin/TCF inhibitor reverses CTTN-induced cancer stem cell-like properties in HER2+ breast cancer cells in vitro. RNA-seq, Western blot, tumorsphere formation, ALDEFLUOR assay, in vivo xenograft, β-catenin/TCF inhibitor treatment Cancers Medium 36831511
2024 Matrix stiffness promotes cell migration, invasion, and invadopodia formation in nasopharyngeal carcinoma through upregulation of wild-type CTTN (WT-CTTN). The splicing factor PTBP2 (activated by high stiffness) controls the production of WT-CTTN over splice variants (SV1-CTTN, SV2-CTTN), establishing a PTBP2–WT-CTTN axis in mechanosensing. siRNA knockdown, overexpression studies, alternative splicing analysis, migration/invasion/invadopodia assays in varying stiffness conditions Cancer science Medium 38273817
2019 HBx (hepatitis B virus X protein) physically interacts with CTTN in HCC cells (validated by Co-IP and confocal microscopy). The HBx–CTTN interaction upregulates CREB1 and its downstream targets (cyclin D1, MMP-9), promoting cell proliferation and migration. Proteomics, Co-IP, confocal microscopy, cell proliferation/migration assays, cell cycle analysis Cell death & disease Medium 31138777
2024 YWHAG (14-3-3γ) interacts with CTTN and mediates CRC cell proliferation, migration, and invasion by activating Wnt/β-catenin signaling downstream of CTTN. RNA-seq, Co-IP (YWHAG–CTTN interaction), functional cell assays, Wnt signaling readouts Medical oncology Low 38538804
2025 RBMS1 promotes alternative splicing of CTTN to generate a CTTN-Δe11 isoform in cardiomyocytes. This splicing switch activates the PI3K/AKT signaling pathway, causing cytoskeleton and sarcomere damage leading to cardiac hypertrophy. RNA splicing analysis, PI3K/AKT pathway assay, cardiac hypertrophy mouse model, pharmacological inhibition of RBMS1 (nortriptyline) EMBO molecular medicine Medium 41214391
2025 Cortactin localizes to Rab7-positive late endosomes and is required for late endosomal tethering and homeostasis. Cortactin depletion in circulating tumor cells causes accumulation of aberrantly enlarged Rab7+ late endosomal aggregates, accumulation and activation of mTOR within these structures, p53 phosphorylation (Ser15, Ser33), G0/G1 cell cycle arrest, and cellular senescence (characterized by SASP, β-galactosidase activity, Ki-67/Lamin B1 depletion, elevated mitochondrial ROS). A positive p53–mtROS feedback loop maintains stable senescence. Live imaging and fractionation (Rab7/mTOR localization), CTTN depletion, p53 phosphorylation assay, cell cycle analysis, senescence markers (β-gal, SASP, Ki-67, Lamin B1, mtROS), CDX mouse models bioRxivpreprint Medium bio_10.1101_2025.03.26.645381
2025 Knockdown of CTTN in HNSCC cells reduces FAK expression levels and impairs cytoskeletal formation, thereby reducing anoikis resistance. CTTN silencing also suppresses growth in patient-derived organoids (PDOs). siRNA knockdown, PI staining (apoptosis), immunofluorescence, Western blot (FAK), PDO culture assays Scientific reports Medium 41168318
2025 LAD1 (ladinin-1) binds LINC01305 and together they co-regulate phosphorylation of CTTN and N-WASP, mediating cytoskeletal reorganization and EMT via activation of the PI3K/AKT signaling pathway in ESCC cells. Co-IP (LAD1–LINC01305), phosphorylation assays (CTTN, N-WASP), PI3K/AKT pathway analysis, EMT marker analysis Molecular carcinogenesis Low 39835575
2024 LPS, TNF-α, and high-magnitude cyclic stretch (18% CS) significantly increase CTTN promoter activity; these responses require NF-κB response elements in the promoter. The promoter SNP rs34612166 (−212T/C) markedly enhances LPS- and 18%CS-induced CTTN promoter activation. HIF pathway activators and NRF2 also modulate CTTN promoter activity. Demethylation (5'-Aza) increases CTTN promoter activity ~2.9-fold, indicating epigenetic regulation. Luciferase reporter assay with full-length CTTN promoter, site-directed mutagenesis (NF-κB sites and SNP), pharmacological modulators (HIF, NRF2 inhibitors/activators, 5'-Aza), cyclic stretch, immunohistochemistry in LPS-exposed mouse lungs Bioscience reports Medium 39162263

Source papers

Stage 0 corpus · 63 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1992 Identification and cloning of two overexpressed genes, U21B31/PRAD1 and EMS1, within the amplified chromosome 11q13 region in human carcinomas. Oncogene 362 1532244
1995 The involvement of the chromosome 11q13 region in human malignancies: cyclin D1 and EMS1 are two new candidate oncogenes--a review. Gene 242 7607576
2003 Cyclin D1, EMS1 and 11q13 amplification in breast cancer. Breast cancer research and treatment 201 12755491
1993 The product of the EMS1 gene, amplified and overexpressed in human carcinomas, is homologous to a v-src substrate and is located in cell-substratum contact sites. Molecular and cellular biology 152 8474448
2003 FLJ10261 gene, located within the CCND1-EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products. International journal of oncology 146 12739008
2019 Downregulation of RNF128 activates Wnt/β-catenin signaling to induce cellular EMT and stemness via CD44 and CTTN ubiquitination in melanoma. Journal of hematology & oncology 144 30832692
2008 Signaling of cell fate determination by the TPD1 small protein and EMS1 receptor kinase. Proceedings of the National Academy of Sciences of the United States of America 140 18250314
2006 Amplification and overexpression of CTTN (EMS1) contribute to the metastasis of esophageal squamous cell carcinoma by promoting cell migration and anoikis resistance. Cancer research 132 17178864
1998 Overexpression of EMS1/cortactin in NIH3T3 fibroblasts causes increased cell motility and invasion in vitro. Oncogene 126 9681820
1996 Expression of cyclin D1 and EMS1 in bladder tumours; relationship with chromosome 11q13 amplification. Oncogene 125 8622895
1999 Signaling pathways and structural domains required for phosphorylation of EMS1/cortactin. Cancer research 110 10537323
1997 EMS1 amplification can occur independently of CCND1 or INT-2 amplification at 11q13 and may identify different phenotypes in primary breast cancer. Oncogene 84 9380415
2009 Calreticulin promotes cell motility and enhances resistance to anoikis through STAT3-CTTN-Akt pathway in esophageal squamous cell carcinoma. Oncogene 70 19684620
2016 Two SERK Receptor-Like Kinases Interact with EMS1 to Control Anther Cell Fate Determination. Plant physiology 68 27920157
2008 Cortactin (CTTN), N-WASP (WASL), and clathrin (CLTC) are present at podosome-like tubulobulbar complexes in the rat testis. Biology of reproduction 68 18799755
2007 Heterologous production of Pseudomonas aeruginosa EMS1 biosurfactant in Pseudomonas putida. Bioresource technology 61 17611103
2006 Recurrent coamplification of cytoskeleton-associated genes EMS1 and SHANK2 with CCND1 in oral squamous cell carcinoma. Genes, chromosomes & cancer 56 16235239
1996 Amplification and expression of EMS-1 (cortactin) in head and neck squamous cell carcinoma cell lines. Oncogene 54 8552396
2019 EMS1 and BRI1 control separate biological processes via extracellular domain diversity and intracellular domain conservation. Nature communications 50 31519884
2017 Carbonic Anhydrases Function in Anther Cell Differentiation Downstream of the Receptor-Like Kinase EMS1. The Plant cell 45 28522549
1993 Amplified region of chromosome band 11q13 in breast and squamous cell carcinomas encompasses three CpG islands telomeric of FGF3, including the expressed gene EMS1. Genes, chromosomes & cancer 44 7685625
2006 Amplifications of TAOS1 and EMS1 genes in oral carcinogenesis: association with clinicopathological features. Oral oncology 39 17005439
2019 The HBx-CTTN interaction promotes cell proliferation and migration of hepatocellular carcinoma via CREB1. Cell death & disease 36 31138777
1996 Expression and tyrosine phosphorylation of EMS1 in human breast cancer cell lines. International journal of cancer 34 8945620
1998 Characterization of the EMS1 gene and its product, human Cortactin. Cell adhesion and communication 33 9823470
2009 Detection of copy number amplification of cyclin D1 (CCND1) and cortactin (CTTN) in oral carcinoma and oral brushed samples from areca chewers. Oral oncology 27 19666237
2014 MTSS1 suppresses cell migration and invasion by targeting CTTN in glioblastoma. Journal of neuro-oncology 26 25385572
2020 Hypermethylation of UCHL1 Promotes Metastasis of Nasopharyngeal Carcinoma by Suppressing Degradation of Cortactin (CTTN). Cells 25 32120844
2020 Long Noncoding RNA (lncRNA) CTTN-IT1 Elevates Skeletal Muscle Satellite Cell Proliferation and Differentiation by Acting as ceRNA for YAP1 Through Absorbing miR-29a in Hu Sheep. Frontiers in genetics 24 32849826
2023 LncRNA INPP5F ameliorates stress-induced hypertension via the miR-335/Cttn axis in rostral ventrolateral medulla. CNS neuroscience & therapeutics 20 36852438
2011 Cortactin (CTTN) overexpression in osteosarcoma correlates with advanced stage and reduced survival. Cancer biomarkers : section A of Disease markers 19 22297550
2021 EMS1/DLL4-Notch Signaling Axis Augments Cell Cycle-Mediated Tumorigenesis and Progress in Human Adrenocortical Carcinoma. Frontiers in oncology 18 34858850
2020 piR-19166 inhibits migration and metastasis through CTTN/MMPs pathway in prostate carcinoma. Aging 18 32881713
2020 Long Noncoding RNASBF2-AS1 Promotes Gastric Cancer Progression via Regulating miR-545/EMS1 Axis. BioMed research international 14 32626753
2018 MiRNA-545 negatively regulates the oncogenic activity of EMS1 in gastric cancer. Cancer medicine 14 29733519
2018 MiR-448 downregulates CTTN to inhibit cell proliferation and promote apoptosis in glioma. European review for medical and pharmacological sciences 14 29949161
2011 An eQTL-based method identifies CTTN and ZMAT3 as pemetrexed susceptibility markers. Human molecular genetics 14 22171072
2016 Amplification and overexpression of CTTN and CCND1 at chromosome 11q13 in Esophagus squamous cell carcinoma (ESCC) of North Eastern Chinese Population. International journal of medical sciences 13 27877079
2023 CTTN Overexpression Confers Cancer Stem Cell-like Properties and Trastuzumab Resistance via DKK-1/WNT Signaling in HER2 Positive Breast Cancer. Cancers 10 36831511
2024 Stiffness promotes cell migration, invasion, and invadopodia in nasopharyngeal carcinoma by regulating the WT-CTTN level. Cancer science 8 38273817
2022 Engineering Chimeras by Fusing Plant Receptor-like Kinase EMS1 and BRI1 Reveals the Two Receptors' Structural Specificity and Molecular Mechanisms. International journal of molecular sciences 8 35216268
2022 A cortactin CTTN coding SNP contributes to lung vascular permeability and inflammatory disease severity in African descent subjects. Translational research : the journal of laboratory and clinical medicine 7 35181549
2024 YWHAG promotes colorectal cancer progression by regulating the CTTN-Wnt/β-catenin signaling axis. Medical oncology (Northwood, London, England) 6 38538804
2022 CBLC inhibits the proliferation and metastasis of breast cancer cells via ubiquitination and degradation of CTTN. Journal of receptor and signal transduction research 5 36043996
2013 Synergistic effect of EMS1-shRNA and sorafenib on proliferation, migration, invasion and endocytosis of SMMC-7721. Journal of molecular histology 5 24127012
2007 G6PD (AC)n and (CTT)n microsatellites in Mexican Mestizos with common G6PD African variants. Blood cells, molecules & diseases 5 17223593
2012 Association of CTTN polymorphisms with the risk of colorectal cancer. Journal of the Korean Surgical Society 4 22403749
2025 Nuclear MicroRNA-124-3p Promotes Neurite Outgrowth After Spinal Cord Injury by Enhancing Cttn Transcription. Molecular neurobiology 3 40044957
2024 Genetic and epigenetic regulation of cortactin (CTTN) by inflammatory factors and mechanical stress in human lung endothelial cells. Bioscience reports 3 39162263
2013 Combining cortactin and CTTN detection with clinicopathologic features increases effectiveness of survival predictions for patients with resectable hepatocellular carcinoma. Clinical laboratory 2 24409670
2011 [Interference of invasion and metastasis of colorectal cancer cells by EMS1-siRNA encoded by a recombinant lentiviral vector]. Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery 2 22116727
1997 [HS1 and EMS1]. Gan to kagaku ryoho. Cancer & chemotherapy 2 9309140
2025 LINC01305 and LAD1 Co-Regulate CTTN and N-WASP Phosphorylation, Mediating Cytoskeletal Reorganization to Promote ESCC Metastasis. Molecular carcinogenesis 1 39835575
2025 CTTN overexpression in HNSCC inhibits Anoikis-apoptosis. Scientific reports 1 41168318
2025 RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics. EMBO molecular medicine 1 41214391
2024 Combined RNAi of CTTN and FGF2 Modulates Cell Migration, Invasion and G1/S Transition of Hepatocellular Carcinoma through Ras/ERK Signaling Pathway. Current cancer drug targets 1 38031266
2008 [Expression and clinical significance of EMS1 gene in gastric carcinoma]. Ai zheng = Aizheng = Chinese journal of cancer 1 18334127
2026 Transcriptional factor ZMYM3 promotes hepatocellular carcinoma metastasis by upregulating CTTN and inducing invadopodia formation. Cell death & disease 0 41775697
2025 RETRACTION: MiRNA-545 Negatively Regulates the Oncogenic Activity of EMS1 in Gastric Cancer. Cancer medicine 0 40583825
2011 [A pivotal role of cortactin, a CTTN encoding protein, in endocytosis of human colon cancer]. Zhonghua yi xue za zhi 0 21418910
2010 [The expression of EMS1 and DcR3 protein in laryngeal carcinoma and the relation between EMS1 and DcR3]. Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery 0 21395182
2009 [Expression of EMS1 protein and its clinical significance in laryngeal carcinoma]. Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery 0 19947248
2005 [Amplification of EMS1 gene in oral carcinogenesis]. Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology 0 15842855

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