Affinage

ARPC3

Actin-related protein 2/3 complex subunit 3 · UniProt O15145

Round 2 corrected
Length
178 aa
Mass
20.5 kDa
Annotated
2026-04-28
114 papers in source corpus 31 papers cited in narrative 31 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ARPC3 (p21-Arc) is a core subunit of the heptameric Arp2/3 complex that nucleates branched actin filament networks, functioning as a critical interface for WASP/Scar-family nucleation-promoting factors and as a structural stabilizer of the active branch junction conformation. ARPC3 directly binds the CA regions of N-WASP and WAVE2, and independently interacts with the Rac GEF Tiam1, coupling upstream Rho-family GTPase signaling to actin remodeling at lamellipodia, dendritic spines, immune synapses, and sites of vesicle trafficking (PMID:9889097, PMID:16285728, PMID:16599904, PMID:33353942). Genetic ablation of ARPC3 causes embryonic lethality at the blastocyst stage in mice, eliminates lamellipodia in favor of formin-dependent filopodia, disrupts persistent directional migration, and in tissue-specific knockouts leads to loss of dendritic spine synapses with behavioral deficits, impaired epidermal barrier formation via dysregulated YAP, defective endolysosomal trafficking in enterocytes, and failed AT2 stem-cell-mediated lung regeneration (PMID:16880528, PMID:22492726, PMID:23554489, PMID:24043783, PMID:25833710, PMID:38377991). ARPC3 expression is regulated post-transcriptionally by multiple miRNAs (miR-29a/b, miR-124-5p, miR-212-5p, miR-26a-5p), and its downregulation impairs actin-dependent processes including phagocytosis, angiogenesis, myelination, and extravillous trophoblast invasion (PMID:21930776, PMID:31636629, PMID:37027305, PMID:40626921).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1997 High

    Identification of ARPC3 as a constituent of the Arp2/3 complex established that actin nucleation at dynamic assembly sites (lamellipodia, pathogen tails) is carried out by a defined multi-subunit machine rather than by individual actin-binding proteins.

    Evidence Protein purification and immunofluorescence in fibroblasts and Listeria-infected cells; reconstitution of ActA-dependent actin polymerization with purified complex

    PMID:9000076 PMID:9230079

    Open questions at the time
    • Role of ARPC3 specifically versus other subunits was not resolved
    • No structural information on subunit contacts
  2. 1998 High

    The discovery that ARPC3 directly binds the C-terminal domains of WASP and Scar1 answered how nucleation-promoting factors activate the Arp2/3 complex, identifying ARPC3 as a key receptor subunit for upstream signals.

    Evidence Yeast two-hybrid, deletion mapping, and dominant-negative overexpression abolishing lamellipodia in cultured cells

    PMID:9889097

    Open questions at the time
    • Binding stoichiometry and affinity not determined
    • Whether other subunits also contribute to NPF binding was unclear
  3. 2001 High

    The 2.0 Å crystal structure of the Arp2/3 complex placed ARPC3 as a globular α-helical subunit and revealed the spatial arrangement needed for branch nucleation, providing an atomic framework for understanding activation.

    Evidence X-ray crystallography of bovine Arp2/3 complex

    PMID:11721045

    Open questions at the time
    • Structure was of the inactive complex; active/branch-bound conformation unknown
    • ARPC3–NPF interface not resolved at atomic detail
  4. 2005 High

    NMR and cross-linking studies mapped the precise contact sites of N-WASP CA peptides on ARPC3 within the intact complex, resolving which surfaces of ARPC3 mediate NPF recognition.

    Evidence Methyl-TROSY NMR, site-directed spin labeling, and chemical cross-linking on purified Arp2/3 complex

    PMID:16285728

    Open questions at the time
    • Full structural model of NPF-bound active complex not yet available
    • Whether contacts differ between WASP-family members was untested
  5. 2006 High

    Two advances established ARPC3's biological indispensability: Arpc3-null mice die at the blastocyst stage with absent peripheral F-actin meshwork, and Tiam1 was shown to bind ARPC3 directly, linking Rac GEF signaling to Arp2/3 activation.

    Evidence Transposon insertional mutagenesis knockout in mice with blastocyst culture/EM; yeast two-hybrid/Co-IP identifying Tiam1–ARPC3 interaction

    PMID:16599904 PMID:16880528

    Open questions at the time
    • How Tiam1–ARPC3 binding relates to Tiam1–Rac catalytic cycle was not resolved
    • Whether other Arp2/3 subunits can partially compensate in vivo was unknown
  6. 2011 High

    Three studies collectively extended ARPC3 function beyond lamellipodia: it is essential for endocytic patch dynamics in fission yeast (rescued by human ARPC3), required for asymmetric oocyte division and actin cap formation, and necessary for branched actin architecture in dendritic spines (miR-29a/b regulation).

    Evidence Cross-species complementation in S. pombe; siRNA in mouse oocytes with live imaging; miR-29a/b target validation and spine morphology in hippocampal neurons

    PMID:21449051 PMID:21494665 PMID:21930776

    Open questions at the time
    • Molecular basis of ARPC3 requirement in endocytosis versus actin nucleation per se was unclear
    • Whether spine phenotype is cell-autonomous in vivo was untested
  7. 2012 High

    Isogenic ARPC3-null fibroblasts demonstrated that loss of Arp2/3-dependent lamellipodia forces cells to rely on formin-driven filopodia for protrusion, establishing the molecular basis for the persistent directional migration defect.

    Evidence ARPC3-knockout mouse ESC-derived fibroblasts, TIRF microscopy, formin localization, quantitative migration assays

    PMID:22492726

    Open questions at the time
    • Whether the filopodia-dominant phenotype is universal across cell types was not addressed
    • Contribution of myosin II compensation was not yet fully dissected
  8. 2013 High

    Tissue-specific knockouts revealed that ARPC3 has non-redundant roles beyond migration: in forebrain neurons it maintains spine synapses and cognitive/social behavior; in epidermis it is required for tight junction assembly and barrier function via YAP regulation.

    Evidence Conditional knockout mice (CamKII-Cre for neurons, K14-Cre for epidermis), electron microscopy, behavioral testing, YAP inhibition rescue

    PMID:23554489 PMID:24043783

    Open questions at the time
    • Mechanism connecting ARPC3 to YAP activity was not fully elucidated
    • Whether spine loss is due to formation or maintenance defect was uncertain
  9. 2015 High

    Conditional knockout in enterocytes showed ARPC3-dependent Arp2/3 activity is required for endolysosomal organization, IgG transcytosis, and lipid absorption, establishing a vesicle trafficking role distinct from cell migration.

    Evidence Intestinal epithelium-specific Arpc3 knockout mouse with transcytosis and lipid absorption assays

    PMID:25833710

    Open questions at the time
    • Whether ARPC3 acts on specific vesicle populations or generally on actin-dependent trafficking was unknown
    • Direct visualization of Arp2/3 on endosomal membranes not shown
  10. 2020 High

    Cryo-electron tomography of branch junctions in cells revealed that ARPC3 makes previously unrecognized contacts with the mother filament and stabilizes the active Arp2/3 conformation, providing the first in-cell structural view of ARPC3's role at the branch point.

    Evidence Cryo-ET with subtomogram averaging at 9.0 Å resolution in intact cells

    PMID:33353942

    Open questions at the time
    • Resolution insufficient for side-chain contacts
    • How ARPC3 conformational change couples to NPF release was unresolved
  11. 2020 High

    Circuit-selective ArpC3 knockout in prefrontal-to-amygdala projection neurons causally linked ARPC3 to circuit excitability and social behavior, with optogenetic rescue confirming necessity and sufficiency.

    Evidence Circuit-selective conditional knockout, in vivo electrophysiology, optogenetic activation and silencing, behavioral testing

    PMID:32726629

    Open questions at the time
    • Molecular identity of affected synapses (excitatory vs. inhibitory remodeling) not resolved
    • Translational relevance to human psychiatric conditions unestablished
  12. 2024 High

    Live imaging of ArpC3-depleted AT2 stem cells in injured lungs showed ARPC3 is required for inter-alveolar migration and regeneration, extending its role to tissue repair via stem cell motility.

    Evidence Longitudinal live imaging of murine lung ex vivo and in vivo with conditional Arpc3 depletion

    PMID:38377991

    Open questions at the time
    • Whether ARPC3 also regulates AT2 differentiation independently of migration was not tested
    • Applicability to human lung regeneration unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • A high-resolution structure of the NPF-bound, active ARPC3-containing Arp2/3 complex at the branch junction — resolving side-chain contacts between ARPC3, the mother filament, and NPFs — and the mechanism by which ARPC3 loss triggers compensatory formin/myosin pathways remain to be determined.
  • No atomic-resolution structure of ARPC3 in the active/NPF-bound branch junction
  • Mechanism linking ARPC3 to YAP signaling in epithelia is not molecularly defined
  • Whether ARPC3 variants contribute to human peripheral neuropathy (CMT) requires functional validation

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 4 GO:0005198 structural molecule activity 2
Localization
GO:0005856 cytoskeleton 6 GO:0005886 plasma membrane 3 GO:0005829 cytosol 2 GO:0031410 cytoplasmic vesicle 2 GO:0005768 endosome 1
Pathway
R-HSA-1266738 Developmental Biology 3 R-HSA-162582 Signal Transduction 2 R-HSA-168256 Immune System 2 R-HSA-5653656 Vesicle-mediated transport 2 R-HSA-1500931 Cell-Cell communication 1
Partners
ARPC4IFT20N-WASPTIAM1WASPWAVE2
Complex memberships
Arp2/3 complex

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 ARPC3 (p21-Arc) was identified as one of seven subunits of the human Arp2/3 complex, and was localized to lamellipodia of stationary and locomoting fibroblasts as well as Listeria monocytogenes actin tails, demonstrating its presence at sites of dynamic actin assembly. Protein purification, amino acid sequencing, immunofluorescence localization The Journal of cell biology High 9230079
1997 The purified Arp2/3 complex, of which ARPC3 (p21-Arc) is a subunit, is sufficient to initiate ActA-dependent actin polymerization at the surface of Listeria monocytogenes and is required for actin tail formation and bacterial motility. Protein purification, in vitro actin polymerization reconstitution assay Nature High 9000076
1998 ARPC3 (p21 subunit) of the Arp2/3 complex directly binds to WASP and Scar1 proteins through their carboxyl-terminal domains; overexpression of this binding domain disrupts Arp2/3 complex localization and abolishes lamellipodia formation. Yeast two-hybrid, deletion analysis, overexpression in cells with immunofluorescence Current biology : CB High 9889097
2001 Crystal structure of bovine Arp2/3 complex at 2.0 Å resolution revealed that ARPC3 (p21) is a globular alpha-helical subunit positioned in the complex; the structure predicted that WASp/Scar proteins activate the complex by bringing Arp2 into proximity with Arp3 for branch nucleation. X-ray crystallography Science (New York, N.Y.) High 11721045
2001 Yeast two-hybrid analysis of Arp2/3 subunit interactions showed that ARPC3 (p21-Arc) interacts with ARPC4 (p20-Arc), and that structural integrity is important for this association; ARPC4 acts as a hub connecting multiple subunits. Yeast two-hybrid assay Biochemical and biophysical research communications Medium 11162547
2000 ARPC3 (p21-Arc) is recruited to the surface of intracellular Listeria monocytogenes independently of actin polymerization, and this recruitment requires specific arginine residues within the 146-KKRRK-150 motif of the bacterial ActA protein. Immunofluorescence staining with anti-p21-Arc antibody, mutational analysis of ActA, latrunculin B treatment Journal of cell science High 10954425
2000 ARPC3 (p21-Arc) and p34-Arc are concentrated at sites of NGF-stimulated actin polymerization in rat sympathetic neuron growth cones within 1–2 minutes, and their retention at these sites does not require actin polymerization itself. Immunofluorescence in primary neurons, NGF stimulation, latrunculin treatment Journal of neuroscience research Medium 10797548
2001 RNAi knockdown of ARC21 (ARPC3) in HeLa cells identified it as an essential gene; its depletion resulted in impaired cell growth. siRNA knockdown, immunofluorescence, immunoblotting, cell growth assay Journal of cell science Medium 11792820
2005 NMR spectroscopy and site-directed spin labeling of N-WASP peptides showed that the extreme C-terminus of the A region and the C-terminus of the C region of N-WASP are proximal to ARPC3 in the Arp2/3 complex; cross-linking also identified ARPC3 as a contact site for the CA peptide of N-WASP. Methyl-TROSY NMR of intact Arp2/3 complex, site-directed spin labeling, chemical cross-linking Biochemistry High 16285728
2006 Tiam1 (Rac-specific GEF) directly interacts with the ARPC3 (p21-Arc) subunit of the Arp2/3 complex through its N-terminal pleckstrin homology domain and adjacent coiled-coil region; this interaction is required for proper subcellular localization of Tiam1 and its capacity to activate Rac1. Yeast two-hybrid screening, Co-IP, co-localization by immunofluorescence, deletion analysis, WASP inhibitor treatment The Biochemical journal High 16599904
2006 Homozygous disruption of Arpc3 in mice causes embryonic lethality at the blastocyst stage; in vitro culture of Arpc3-null blastocysts showed severe trophoblast spreading impairment, absence of actin-rich structures at the cell periphery, and lack of mesh-like F-actin structures at the periphery visible by electron microscopy. Sleeping Beauty transposon insertional mutagenesis, blastocyst culture, immunofluorescence, electron microscopy Molecular and cellular biology High 16880528
2011 In fission yeast, Arc3 (the ARPC3 ortholog) co-localizes with F-actin patches, is essential for viability, and is required for proper F-actin patch organization, patch mobility, and efficient endocytosis. Human ARPC3 rescues viability of arc3 null mutant and localizes to F-actin patches in human cells. Gene deletion, conditional repression, F-actin imaging, endocytosis assay, cross-species complementation Yeast (Chichester, England) High 21449051
2011 Disruption of Arp2/3 complex by Arpc3 RNAi in mouse oocytes caused failure of asymmetric division, spindle migration defects, disruption of actin cap and cortical granule-free domain formation, and failure of cytokinesis completion during meiotic maturation. siRNA microinjection, immunofluorescence, live imaging, CK666 inhibitor PloS one High 21494665
2011 miR-29a/b directly targets ARPC3 mRNA for downregulation, and this targeting reduces mushroom-shaped dendritic spines on hippocampal neurons with a concomitant increase in filopodial-like outgrowths, indicating that ARPC3 is required for actin network branching in mature synaptic spines. In vitro imaging of hippocampal neurons, miRNA overexpression, target validation, spine morphology analysis The Journal of cell biology High 21930776
2012 ARPC3-null fibroblasts (derived from ARPC3−/− mouse embryonic stem cells) are unable to extend lamellipodia but generate dynamic leading edges composed primarily of filopodia-like protrusions with formin proteins (mDia1, mDia2) concentrated at their tips; these cells show a strong defect in persistent directional migration despite comparable overall migration speed. Isogenic gene disruption in mouse ESCs, live cell imaging, TIRF microscopy, formin localization The Journal of cell biology High 22492726
2012 Nucleation promoting factors JMY and WAVE2 are upstream regulators of Arp2/3 complex in mouse oocytes; knockdown of Arpc2 and Arpc3 did not affect expression or localization of JMY and WAVE2, placing ARPC3 downstream of these NPFs in the actin cap formation pathway. siRNA microinjection, immunofluorescence, immunoblotting in mouse oocytes PloS one Medium 23272233
2013 Postnatal conditional knockout of ArpC3 in forebrain excitatory neurons leads to asymmetric structural plasticity of dendritic spines, progressive loss of spine synapses, and evolution of cognitive, psychomotor, and social behavioral disturbances in mice. Conditional knockout mouse, electron microscopy, spine morphology analysis, behavioral testing The Journal of neuroscience High 23554489
2013 Loss of ArpC3 in epidermis results in defects in tight junction assembly/function, impaired terminal differentiation, and failure to establish an effective epidermal barrier; YAP is inappropriately active in ArpC3-null tissue, and YAP inhibition rescues differentiation and barrier defects. Conditional knockout mouse, barrier assays, immunofluorescence, YAP inhibition rescue experiment Proceedings of the National Academy of Sciences of the United States of America High 24043783
2015 In ARPC3-null fibroblasts, formin-family nucleators are required for extension of filopodia-like protrusions but insufficient to produce a continuous leading edge; myosin II is concentrated in arc-like regions between protrusions and its activity is required for coordinated advancement of the leading edge, explaining the directional migration defect. Arpc3-null fibroblasts, formin inhibitors, myosin II inhibition, live imaging, mathematical modeling with experimental verification Molecular biology of the cell High 25568333
2015 Loss of ArpC3 in intestinal enterocytes causes defects in endolysosomal organization, disruption of IgG transcytosis, and perturbation of lipid absorption, demonstrating that the Arp2/3 complex is required for vesicle trafficking in the intestinal epithelium. Conditional knockout mouse, endolysosomal imaging, transcytosis assay, lipid absorption assay Molecular biology of the cell High 25833710
2014 Knockdown of ARPC3 (or Arp3) in preadipocytes strongly impairs adipocyte differentiation, suppresses formation of F-actin-rich cortical structures at the plasma membrane after adipogenic induction, and reduces GLUT4 vesicle exocytosis and insulin signal transduction. siRNA knockdown, differentiation assay, F-actin imaging, GLUT4 exocytosis assay The Biochemical journal Medium 25220164
2017 IFT20 interacts with ARPC3 in Jurkat T cells (identified by quantitative mass spectrometry), and depletion of ARPC3 by RNAi impairs TCR accumulation and phosphotyrosine signaling at the immune synapse by reducing the ability of endosomal TCRs to polarize to the synapse. Quantitative MS interactome, RNAi depletion, confocal imaging of antigen-specific T cell conjugates Journal of cell science Medium 28154159
2019 miR-124-5p directly targets ARPC3 (and ARPC4) mRNA — confirmed by luciferase reporter assay — and reduces ARPC3 protein levels, leading to decreased phagocytic activity in human macrophages by disrupting actin cytoskeleton dynamics. Luciferase reporter assay, miRNA mimic transfection, Western blotting, phagocytosis assay Frontiers in immunology Medium 31636629
2020 Cryo-electron tomography of the actin filament branch junction in cells revealed a central role for the ArpC3 subunit in stabilizing the active conformation of the Arp2/3 complex within the branch junction, with a previously undescribed set of interactions with the mother filament. Cryo-electron tomography, subtomogram averaging (9.0 Å resolution structure in cells) Nature communications High 33353942
2020 mTOR regulates ARPC3 expression in oligodendrocytes; loss or inhibition of mTOR reduces ARPC3 protein levels, leading to deficits in actin polymerization, reduced oligodendrocyte process branching, and a delay in myelination initiation. Oligodendrocyte-specific mTOR conditional knockout mouse, Western blotting, phalloidin staining, primary OPC culture with mTOR inhibition The Journal of neuroscience Medium 32139584
2020 Circuit-selective knockout of ArpC3 in prefrontal cortical neurons projecting to the basolateral amygdala elevates circuit neuron excitability, disrupts socially evoked neural activity, and produces abnormal social behavior; optogenetic activation of this circuit in wild-type mice recapitulates the social dysfunction, and optogenetic silencing rescues it in knockout mice. Circuit-selective conditional knockout, in vivo electrophysiology, optogenetics, behavioral testing Cell reports High 32726629
2023 VEGF-A stimulation induces nuclear translocation of cGAS via importin-β, after which nuclear cGAS regulates a miR-212-5p–ARPC3 cascade to modulate VEGF-A-mediated angiogenesis by affecting cytoskeletal dynamics and VEGFR2 trafficking from the trans-Golgi network to the plasma membrane. In vitro angiogenesis assays, VEGF-A stimulation, cGAS overexpression/knockout, miRNA manipulation, VEGFR2 trafficking assays Cell reports Medium 37027305
2024 Genetic depletion of ArpC3 in alveolar type 2 (AT2) stem cells inhibits their migration between alveolar units and impairs regeneration of both AT2 and AT1 cells in vivo following lung injury. Longitudinal live imaging of murine lung ex vivo and in vivo, ArpC3 conditional genetic depletion, cell tracking Developmental cell High 38377991
2025 ARPC3 is a direct target of miR-26a-5p (validated by luciferase reporter assay); knockdown of ARPC3 in EVT cells mimics miR-26a-5p overexpression by impairing invasion/migration and transforming lamellipodia to filopodia at the leading edge, linking the miR-26a-5p/ARPC3 axis to disrupted actin cytoskeletal organization and impaired directional EVT invasion in preeclampsia. Luciferase reporter assay, ARPC3 siRNA knockdown, ARPC3 ectopic overexpression rescue, live cell imaging, EVT invasion/migration assays Biology of reproduction Medium 40626921
2022 The SARS-CoV-2 spike protein cytoplasmic tail contains a V1264L1265 intracellular targeting motif that interacts with ARPC3 (among other host proteins) to regulate spike protein transport and subcellular localization; reducing ARPC3 expression significantly represses live SARS-CoV-2 virion assembly. CT deletion/mutation constructs, pseudovirus and live virus assembly assays, co-immunoprecipitation, ARPC3 knockdown Antiviral research Medium 36572190
2025 Rare variant burden analysis in 34,851 cases identified ARPC3 as a candidate disease gene for Charcot-Marie-Tooth disease, providing human genetic evidence linking ARPC3 loss-of-function to a peripheral neuropathy. Whole-genome sequencing rare variant gene burden analysis (100,000 Genomes Project) Nature Low 40011789

Source papers

Stage 0 corpus · 114 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2005 Nucleolar proteome dynamics. Nature 934 15635413
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2009 A genome-wide RNAi screen identifies multiple synthetic lethal interactions with the Ras oncogene. Cell 843 19490893
2002 Directed proteomic analysis of the human nucleolus. Current biology : CB 780 11790298
1998 Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex. Current biology : CB 761 9889097
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2001 Identification of essential genes in cultured mammalian cells using small interfering RNAs. Journal of cell science 703 11792820
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2008 Large-scale proteomics and phosphoproteomics of urinary exosomes. Journal of the American Society of Nephrology : JASN 607 19056867
2017 Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science (New York, N.Y.) 533 28302793
1997 Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes. Nature 494 9000076
2011 Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation. Nature cell biology 490 21423176
2003 Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nature biotechnology 485 12665801
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2015 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell 433 26638075
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
1997 The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. The Journal of cell biology 430 9230079
2001 Crystal structure of Arp2/3 complex. Science (New York, N.Y.) 413 11721045
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2000 Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex. The Journal of cell biology 355 11018051
2016 Vessel co-option mediates resistance to anti-angiogenic therapy in liver metastases. Nature medicine 337 27748747
2012 The Arp2/3 complex is required for lamellipodia extension and directional fibroblast cell migration. The Journal of cell biology 281 22492726
2001 Interaction of WASP/Scar proteins with actin and vertebrate Arp2/3 complex. Nature cell biology 277 11146629
2012 A high-throughput approach for measuring temporal changes in the interactome. Nature methods 273 22863883
2011 A directed protein interaction network for investigating intracellular signal transduction. Science signaling 258 21900206
2007 hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. Genomics 222 17207965
2011 Arp2/3 complex regulates asymmetric division and cytokinesis in mouse oocytes. PloS one 143 21494665
2013 Disruption of Arp2/3 results in asymmetric structural plasticity of dendritic spines and progressive synaptic and behavioral abnormalities. The Journal of neuroscience : the official journal of the Society for Neuroscience 141 23554489
2011 Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology. The Journal of cell biology 116 21930776
2006 SRY-box containing gene 11 (Sox11) transcription factor is required for neuron survival and neurite growth. Neuroscience 115 17055661
2013 Phlegm-dampness constitution: genomics, susceptibility, adjustment and treatment with traditional Chinese medicine. The American journal of Chinese medicine 75 23548117
2013 Actin-related protein2/3 complex regulates tight junctions and terminal differentiation to promote epidermal barrier formation. Proceedings of the National Academy of Sciences of the United States of America 67 24043783
2015 SCARN a Novel Class of SCAR Protein That Is Required for Root-Hair Infection during Legume Nodulation. PLoS genetics 64 26517270
2020 Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. Nature communications 61 33353942
2013 Silencing of the ARP2/3 complex disturbs pancreatic cancer cell migration. Anticancer research 56 23267127
2009 Detection and expression of the phosphonate transporter gene phnD in marine and freshwater picocyanobacteria. Environmental microbiology 56 19220397
2006 Interaction between Tiam1 and the Arp2/3 complex links activation of Rac to actin polymerization. The Biochemical journal 47 16599904
2000 Mutations of arginine residues within the 146-KKRRK-150 motif of the ActA protein of Listeria monocytogenes abolish intracellular motility by interfering with the recruitment of the Arp2/3 complex. Journal of cell science 46 10954425
2006 Sleeping beauty transposon-based phenotypic analysis of mice: lack of Arpc3 results in defective trophoblast outgrowth. Molecular and cellular biology 44 16880528
2015 The Arp2/3 complex has essential roles in vesicle trafficking and transcytosis in the mammalian small intestine. Molecular biology of the cell 43 25833710
1992 Hemophilia A due to mutations that create new N-glycosylation sites. Proceedings of the National Academy of Sciences of the United States of America 43 1594597
2015 A mechanism of leading-edge protrusion in the absence of Arp2/3 complex. Molecular biology of the cell 42 25568333
2020 Mechanistic Target of Rapamycin Regulates the Oligodendrocyte Cytoskeleton during Myelination. The Journal of neuroscience : the official journal of the Society for Neuroscience 36 32139584
2005 NMR analyses of the activation of the Arp2/3 complex by neuronal Wiskott-Aldrich syndrome protein. Biochemistry 35 16285728
2020 Dysregulation of the Synaptic Cytoskeleton in the PFC Drives Neural Circuit Pathology, Leading to Social Dysfunction. Cell reports 29 32726629
2000 Recruitment of the Arp2/3 complex and mena for the stimulation of actin polymerization in growth cones by nerve growth factor. Journal of neuroscience research 29 10797548
2020 Exosomes Derived from the Human Primary Colorectal Cancer Cell Line SW480 Orchestrate Fibroblast-Led Cancer Invasion. Proteomics 28 32438511
2014 Arp2/3 complex regulates adipogenesis by controlling cortical actin remodelling. The Biochemical journal 26 25220164
2017 Identification of salivary protein biomarkers for orthodontically induced inflammatory root resorption. Proteomics. Clinical applications 25 28371361
2010 Antiparkinsonian trophic action of glial cell line-derived neurotrophic factor and transforming growth factor β1 is enhanced after co-infusion in rats. Experimental neurology 25 20713051
2017 The T cell IFT20 interactome reveals new players in immune synapse assembly. Journal of cell science 24 28154159
2010 Molecular dynamics simulations of Arp2/3 complex activation. Biophysical journal 24 20959098
2006 Proteomic signatures in laryngeal squamous cell carcinoma. ORL; journal for oto-rhino-laryngology and its related specialties 24 17127822
2019 mir-124-5p Regulates Phagocytosis of Human Macrophages by Targeting the Actin Cytoskeleton via the ARP2/3 Complex. Frontiers in immunology 21 31636629
2016 Identification of reliable reference genes for qRT-PCR studies of the developing mouse mammary gland. Scientific reports 21 27752147
2023 Nuclear translocation of cGAS orchestrates VEGF-A-mediated angiogenesis. Cell reports 20 37027305
2018 Glycerol monolaurate induces filopodia formation by disrupting the association between LAT and SLP-76 microclusters. Science signaling 20 29717064
2021 Biallelic truncating variants in ATP9A cause a novel neurodevelopmental disorder involving postnatal microcephaly and failure to thrive. Journal of medical genetics 19 34379057
2022 Aging-Associated Changes in Oxidative Stress Negatively Impacts the Urinary Bladder Urothelium. International neurourology journal 18 35793989
2024 Stem cell migration drives lung repair in living mice. Developmental cell 17 38377991
2008 Molecular dynamics simulation and coarse-grained analysis of the Arp2/3 complex. Biophysical journal 17 18805923
2019 The tomato Arp2/3 complex is required for resistance to the powdery mildew fungus Oidium neolycopersici. Plant, cell & environment 16 31038756
2017 TaARPC3, Contributes to Wheat Resistance against the Stripe Rust Fungus. Frontiers in plant science 16 28769954
2016 Lecithin Prevents Cortical Cytoskeleton Reorganization in Rat Soleus Muscle Fibers under Short-Term Gravitational Disuse. PloS one 16 27073851
2005 Two closely linked variations in actin cytoskeleton pathway in a Chinese pedigree with disseminated superficial actinic porokeratosis. Journal of the American Academy of Dermatology 16 15928614
2001 Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub. Biochemical and biophysical research communications 16 11162547
2015 Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study. Lipids in health and disease 14 25971815
2013 CaCO3 biomineralization on cyanobacterial surfaces: insights from experiments with three Synechococcus strains. Colloids and surfaces. B, Biointerfaces 14 23899673
2011 In silico analysis of combinatorial microRNA activity reveals target genes and pathways associated with breast cancer metastasis. Cancer informatics 14 21552493
2014 Effects of acute and repeated cocaine on markers for neural plasticity within the mesolimbic system in rats. Psychopharmacology 13 24912888
2012 Nucleation promoting factors regulate the expression and localization of Arp2/3 complex during meiosis of mouse oocytes. PloS one 13 23272233
2014 Novel identification of Dermacentor variabilis Arp2/3 complex and its role in rickettsial infection of the arthropod vector. PloS one 12 24733187
2011 Schizosaccharomyces pombe Arc3 is a conserved subunit of the Arp2/3 complex required for polarity, actin organization, and endocytosis. Yeast (Chichester, England) 12 21449051
2018 Transcriptome analysis of adherens junction pathway-related genes after peripheral nerve injury. Neural regeneration research 11 30136696
2016 Protein Interaction Network of Human Protein Kinase D2 Revealed by Chemical Cross-Linking/Mass Spectrometry. Journal of proteome research 11 27559607
2016 A CpG-SNP Located within the ARPC3 Gene Promoter Is Associated with Hypertriglyceridemia in Severely Obese Patients. Annals of nutrition & metabolism 10 27055012
2025 Rare disease gene association discovery in the 100,000 Genomes Project. Nature 9 40011789
2022 Expression of actin- and oxidative phosphorylation-related transcripts across the cortical visuospatial working memory network in unaffected comparison and schizophrenia subjects. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 9 35034100
2022 Effects of lipoteichoic and arachidonic acids on the immune-regulatory mechanism of bovine mammary epithelial cells using multi-omics analysis. Frontiers in veterinary science 9 36090174
2022 A new intracellular targeting motif in the cytoplasmic tail of the spike protein may act as a target to inhibit SARS-CoV-2 assembly. Antiviral research 8 36572190
2016 The role of viral protein Ac34 in nuclear relocation of subunits of the actin-related protein 2/3 complex. Virologica Sinica 8 27900558
2023 Integrative analysis of genes reveals endoplasmic reticulum stress-related immune responses involved in dilated cardiomyopathy with fibrosis. Apoptosis : an international journal on programmed cell death 7 37462883
2024 Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model. Biomolecules 6 39199289
2024 Design, Synthesis, Characterization, and Cytotoxicity of New Pyrazolylmethylene-2-thioxoimidazolidin-4-one Derivatives towards Androgen-Sensitive LNCaP Prostate Cancer Cells. Biomolecules 5 39062524
2022 CRISPR-Cas9 Arabidopsis mutants of genes for ARPC1 and ARPC3 subunits of ARP2/3 complex reveal differential roles of complex subunits. Scientific reports 5 36307477
2025 Cytochalasin B Mitigates the Inflammatory Response in Lipopolysaccharide-Induced Mastitis by Suppressing Both the ARPC3/ARPC4-Dependent Cytoskeletal Changes and the Association Between HSP70 and the NLRP3 Inflammasome. International journal of molecular sciences 4 40243637
2024 Combining single-cell RNA sequencing and network pharmacology to explore the target of cangfu daotan decoction in the treatment of obese polycystic ovary syndrome from an immune perspective. Frontiers in pharmacology 4 39539629
2023 iTRAQ and PRM-Based Comparative Proteomic Profiling of the Hippocampus in Rat Models of Epilepsy. Journal of integrative neuroscience 4 36722232
2022 Raloxifene Suppresses Tumor Growth and Metastasis in an Orthotopic Model of Castration-Resistant Prostate Cancer. Biomedicines 4 35453603
2022 Analysis of the multi-physiological and functional mechanism of wheat alkylresorcinols based on reverse molecular docking and network pharmacology. Food & function 4 35943408
2018 Frazzled can act through distinct molecular pathways in epithelial cells to regulate motility, apical constriction, and localisation of E-Cadherin. PloS one 4 29518139
2009 Reassessment of microarray expression data of porokeratosis by quantitative real-time polymerase chain reaction. Journal of cutaneous pathology 4 19615027
2025 Single-cell hdWGCNA reveals a novel diagnostic model and signature genes of macrophages associated with chronic obstructive pulmonary disease. Inflammation research : official journal of the European Histamine Research Society ... [et al.] 3 40244418
2019 Expression of progesterone receptor, estrogen receptors α and β, and kisspeptin in the hypothalamus during perinatal development of gonad-lacking steroidogenic factor-1 knockout mice. Brain research 3 30776325
2002 Characterization of Arp2/3 complex in chicken tissues. Cell structure and function 3 12502893
2025 Compound kushen injection inhibits breast cancer lung metastasis through regulating MTSS1/ARPC3/F-actin. Journal of ethnopharmacology 2 40446975
2016 CONTENT OF CYTOSKELETON PROTEINS IN OOCYTES OF THIRD GENERATION DROSOPHILA MELANOGASTER FRUIT FLIES OBTAINED AFTER A 44.5-DAY SPACE FLIGHT. Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine 2 29873978
2025 Salivary and gingival crevicular fluid (GCF) protein biomarkers as proxies of root resorption following orthodontic tooth movement. A systematic review. Journal of clinical and experimental dentistry 1 40485969
2026 Integrated single-cell and spatial transcriptomics combined with whole-exome sequencing reveal key hub genes and epithelial heterogeneity in bladder cancer. Frontiers in oncology 0 41669266
2026 Cell type resolved MR based on brain single cell eQTLs corroborated by single cell RNA sequencing uncovers neuroimmune and vascular programs in intracerebral hemorrhage. Journal of translational medicine 0 41673661
2025 Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis. Biomolecules 0 40305275
2025 Liangxue Qushi Zhiyang Decoction Inhibits Atopic Dermatitis in Mice via FcγR-Mediated Phagocytosis. Mediators of inflammation 0 40322063
2025 Exosomal Gene Biomarkers in Osteosarcoma: Mifepristone as a Targeted Therapeutic Revealed by Multi-Omics Analysis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 0 40616392
2025 Human extravillous trophoblast invasion and migration are impeded by excessive miR-26a-5p: dysregulated actin cytoskeleton in extravillous trophoblasts during early-onset severe preeclampsia†. Biology of reproduction 0 40626921
2022 Application of iTRAQ Technology to Identify Differentially Expressed Proteins of Sauce Lamb Tripe with Different Secondary Pasteurization Treatments. Foods (Basel, Switzerland) 0 35454754
2015 Construction of a full-length cDNA library and preliminary analysis of expressed sequence tags from lymphocytes of half-pipe snowboarding athletes. Genetics and molecular research : GMR 0 26505445