Affinage

ARPC3

Actin-related protein 2/3 complex subunit 3 · UniProt O15145

Length
178 aa
Mass
20.5 kDa
Annotated
2026-06-09
86 papers in source corpus 30 papers cited in narrative 30 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ARPC3 (p21-Arc) is an essential subunit of the heteroheptameric Arp2/3 complex that drives nucleation of branched actin networks at the leading edge of motile cells, where it localizes alongside other Arp2/3 subunits to lamellipodia and to Listeria monocytogenes actin tails but not to stress fibers (PMID:9230079). High-resolution crystallography established ARPC3 as a globular alpha-helical subunit within the assembled complex (PMID:11721045), and in-cell cryo-electron tomography of branch junctions showed that it plays a central structural role in stabilizing the active conformation of the complex at the actin branch point (PMID:33353942). ARPC3 forms part of the binding interface for activating nucleation-promoting factors, with the C-terminal A and C regions of N-WASP VCA lying proximal to it in the complex (PMID:16285728). ARPC3 is required for branched (Y-branch) actin formation: its genetic loss abolishes lamellipodia, leaving cells to extend formin-driven filopodia-like protrusions that migrate at normal speed but fail at persistent directional migration because they lack a coordinated leading edge, a defect that also depends on myosin II for advancing inter-protrusion regions (PMID:16880528, PMID:22492726, PMID:25568333). The complementation of an S. pombe arc3 null by human ARPC3 confirms deep functional conservation of this role in F-actin patch assembly and endocytosis (PMID:21449051). Beyond cortical actin, ARPC3 supports vesicle and endosomal trafficking — organizing the endolysosomal system for IgG transcytosis and lipid absorption in enterocytes, polarizing endosomal TCR delivery to the immune synapse through interaction with IFT20, and supporting GLUT4 exocytosis during adipogenesis (PMID:25833710, PMID:28154159, PMID:25220164). Through these activities ARPC3 is essential for embryonic viability and trophoblast outgrowth (PMID:16880528), oocyte asymmetric division and spindle migration (PMID:21494665), dendritic spine maintenance and circuit-level social behavior (PMID:23554489, PMID:32726629), epidermal tight-junction assembly and barrier formation upstream of YAP (PMID:24043783), and lung alveolar stem cell migration during regeneration (PMID:38377991). ARPC3 expression is controlled post-transcriptionally by multiple microRNAs — miR-29a/b, miR-124-5p, miR-26a-5p, and a VEGF-A/nuclear-cGAS/miR-212-5p cascade — that tune actin branching in contexts ranging from dendritic spine morphology to macrophage phagocytosis, trophoblast invasion, and angiogenesis (PMID:21930776, PMID:31636629, PMID:40626921, PMID:37027305), and it lies downstream of mTOR signaling in oligodendrocyte differentiation (PMID:32139584). ARPC3 also acts as a host factor exploited by SARS-CoV-2 spike for virion assembly (PMID:36572190).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1997 High

    Establishing ARPC3 as a bona fide Arp2/3 subunit localized to sites of dynamic actin assembly defined the complex's physiological context and pointed to branched actin nucleation at the leading edge.

    Evidence Protein complex purification and immunofluorescence in fibroblasts and Listeria-infected cells

    PMID:9230079

    Open questions at the time
    • Localization alone did not define ARPC3's specific structural or catalytic contribution within the complex
    • No mechanism for recruitment to dynamic actin sites
  2. 2000 Medium

    Identifying the ActA arginine-rich motif required to recruit p21-Arc to Listeria, independent of actin polymerization, showed Arp2/3 recruitment is an upstream event with ARPC3 as a detectable marker.

    Evidence ActA site-directed mutagenesis, anti-p21-Arc immunofluorescence, latrunculin B treatment

    PMID:10954425

    Open questions at the time
    • Did not establish whether ARPC3 itself contacts ActA versus another subunit
    • Recruitment in the native host context unaddressed
  3. 2001 High

    Crystallography placed ARPC3 as a globular alpha-helical subunit in the assembled complex and framed the WASp/Scar activation model, providing the structural baseline for understanding ARPC3's role.

    Evidence X-ray crystallography of bovine Arp2/3 at 2.0 Å

    PMID:11721045

    Open questions at the time
    • The inactive crystal did not show the active branch-junction conformation
    • Did not directly localize the NPF binding interface
  4. 2001 Medium

    RNAi knockdown causing impaired growth and a yeast two-hybrid ARPC3–ARPC4 interaction began to define ARPC3 essentiality and an internal assembly interface.

    Evidence siRNA knockdown with growth assay; yeast two-hybrid of human subunits

    PMID:11162547 PMID:11792820

    Open questions at the time
    • Y2H interaction was single-method without biochemical validation (Low confidence)
    • Growth defect not mechanistically attributed to a specific actin function
  5. 2005 High

    Mapping N-WASP VCA C-terminal regions proximal to ARPC3 implicated it directly in the activating-NPF binding interface, connecting structure to the activation mechanism.

    Evidence Site-directed spin labeling, methyl-TROSY NMR, chemical cross-linking/MS on intact complex

    PMID:16285728

    Open questions at the time
    • Proximity mapping did not yield atomic-resolution contacts
    • Functional necessity of the ARPC3–VCA contact for nucleation not isolated
  6. 2006 High

    Demonstrating direct Tiam1 binding to p21-Arc that co-localizes Tiam1 with Arp2/3 and is needed for Rac1 activation revealed ARPC3 as a scaffolding interface coupling Rac signaling to actin assembly.

    Evidence Yeast two-hybrid, reciprocal Co-IP, pulldown, deletion mutagenesis, co-localization

    PMID:16599904

    Open questions at the time
    • Whether this interface is occupied simultaneously with NPF binding unknown
    • In vivo significance across tissues not tested
  7. 2006 High

    Knockout embryonic lethality at blastocyst stage with loss of peripheral Y-branched actin established ARPC3 as obligatory for branched actin nucleation and trophoblast outgrowth in vivo.

    Evidence Transposon and conventional gene targeting in mice, blastocyst culture, immunofluorescence, electron microscopy

    PMID:16880528

    Open questions at the time
    • Early lethality precluded analysis of later developmental roles
    • Did not resolve which downstream actin structures cause each defect
  8. 2011 High

    Cross-species complementation in S. pombe and oocyte RNAi extended ARPC3's essential branched-actin role to endocytosis, F-actin patch assembly, and asymmetric cell division, demonstrating deep conservation.

    Evidence S. pombe gene deletion with human ARPC3 rescue; oocyte siRNA with CK666 inhibition and live imaging

    PMID:21449051 PMID:21494665

    Open questions at the time
    • Mechanistic coupling of Arp2/3 to spindle migration not detailed
    • Conservation of regulatory inputs across species untested
  9. 2012 High

    Isogenic ARPC3-null fibroblasts revealed that loss of lamellipodia leaves formin-driven filopodia intact, dissecting ARPC3's specific role in persistent directional migration versus migration speed.

    Evidence ARPC3-/- ES-derived fibroblasts, live imaging, migration assays; NPF epistasis (JMY/WAVE2) in oocytes

    PMID:22492726 PMID:23272233

    Open questions at the time
    • How leading-edge coordination is lost mechanistically not yet resolved
    • Relative contributions of branched versus linear actin to overall motility unclear
  10. 2013 High

    Conditional knockouts in neurons and epidermis tied ARPC3-dependent actin to dendritic spine maintenance and synaptic/behavioral function, and to tight-junction assembly and barrier formation upstream of YAP.

    Evidence Cre-lox conditional knockouts, electron microscopy, behavioral testing, barrier assays, YAP inhibition rescue

    PMID:23554489 PMID:24043783

    Open questions at the time
    • Direct molecular link between Arp2/3 actin and YAP regulation not defined
    • Whether spine loss is primary or secondary to plasticity defects unresolved
  11. 2015 High

    Enterocyte and migration-modeling studies distinguished ARPC3's vesicle-trafficking role (endolysosomal organization, transcytosis, lipid absorption) from cortical actin and showed myosin II cooperates with formins for leading-edge advance.

    Evidence Intestine-specific knockout with trafficking assays; ARPC3-null fibroblasts with formin/myosin inhibition and mathematical modeling

    PMID:25568333 PMID:25833710

    Open questions at the time
    • How Arp2/3 organizes the endolysosomal system mechanistically not defined
    • Identity of specific cargoes dependent on ARPC3 incomplete
  12. 2017 Medium

    Identifying ARPC3–IFT20 interaction required for endosomal TCR polarization at the immune synapse extended ARPC3's trafficking role to adaptive immune signaling.

    Evidence Quantitative MS interactome, RNAi knockdown, confocal imaging of T cell conjugates

    PMID:28154159

    Open questions at the time
    • Single-lab interactome without reciprocal structural validation of the ARPC3–IFT20 contact
    • Whether the interaction is direct or complex-mediated unresolved
  13. 2011 High

    Demonstrating direct miR-29a/b targeting of the Arpc3 3'UTR controlling dendritic spine morphology opened post-transcriptional regulation as a major control layer for ARPC3-driven actin branching.

    Evidence Luciferase 3'UTR reporter, Western blotting, dendritic spine imaging in hippocampal neurons

    PMID:21930776

    Open questions at the time
    • Whether endogenous miR-29 sets ARPC3 levels physiologically not shown
    • Did not address other miRNA inputs
  14. 2024 High

    A series of studies established multiple miRNA inputs (miR-124-5p, miR-26a-5p, cGAS/miR-212-5p) and mTOR and MTSS1 signaling as upstream regulators tuning ARPC3 across phagocytosis, trophoblast invasion, angiogenesis, myelination, and cancer metastasis, and confirmed ARPC3-dependent migration in lung stem cell regeneration and as a SARS-CoV-2 assembly host factor.

    Evidence Luciferase reporters, miRNA mimics/inhibitors, knockdown/overexpression rescue, conditional knockout with intravital imaging, Co-IP and viral assembly assays

    PMID:31636629 PMID:32139584 PMID:36572190 PMID:37027305 PMID:38377991 PMID:40446975 PMID:40626921

    Open questions at the time
    • Several pathway placements (mTOR, MTSS1, cGAS) are multi-step and indirect
    • Direct biochemical mechanism of ARPC3 in viral assembly not resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How ARPC3's structural stabilization of the active branch junction is gated by the diverse upstream inputs (NPFs, Tiam1, miRNAs, kinase signaling) to produce context-specific actin outcomes remains unresolved.
  • No integrated model linking ARPC3 regulatory inputs to distinct downstream actin architectures
  • Atomic-resolution map of all ARPC3 interaction surfaces incomplete
  • Tissue-specific necessity of trafficking versus cortical-actin functions not fully delineated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 3 GO:0005198 structural molecule activity 2 GO:0060090 molecular adaptor activity 2
Localization
GO:0005768 endosome 2 GO:0005794 Golgi apparatus 2 GO:0005856 cytoskeleton 2 GO:0005829 cytosol 1
Pathway
R-HSA-1266738 Developmental Biology 3 R-HSA-9609507 Protein localization 3 R-HSA-5653656 Vesicle-mediated transport 2
Partners
ARPC4IFT20PKD2TIAM1WASL
Complex memberships
Arp2/3 complex

Evidence

Reading pass · 30 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 ARPC3 (p21-Arc) was identified as one of seven subunits of the human Arp2/3 complex; p21-Arc, Arp3, and p34-Arc were localized to lamellipodia of stationary and locomoting fibroblasts and to Listeria monocytogenes actin tails, but not to actin stress fibers, establishing the complex's role in dynamic actin assembly at the leading edge. Protein complex purification, sequence determination, immunofluorescence localization in cells and Listeria-infected cells The Journal of cell biology High 9230079
2001 Crystal structure of bovine Arp2/3 complex at 2.0 Å resolution revealed ARPC3 (p21) as a globular alpha-helical subunit positioned in the complex; structural analysis predicted that WASp/Scar proteins activate the complex by repositioning Arp2 near Arp3 for actin filament branch nucleation. X-ray crystallography (2.0 Å resolution) Science (New York, N.Y.) High 11721045
2001 RNAi knockdown of ARC21 (ARPC3) in mammalian cultured cells caused impaired cell growth, identifying ARPC3 as an essential gene for cell viability in tissue culture. siRNA-mediated knockdown, immunofluorescence, immunoblotting, cell growth assay Journal of cell science Medium 11792820
2001 Yeast two-hybrid analysis of human Arp2/3 subunits showed that p21-Arc (ARPC3) directly interacts with p20-Arc (ARPC4); structural integrity was important for the p20-Arc/p21-Arc association, suggesting a specific assembly interface between these subunits. Yeast two-hybrid assay Biochemical and biophysical research communications Low 11162547
2000 Immunofluorescence with anti-p21-Arc (ARPC3) antibodies showed that the initial recruitment of the Arp2/3 complex to the surface of Listeria monocytogenes requires arginine residues within the 146-KKRRK-150 motif of ActA, and that this recruitment precedes and is independent of actin polymerization. Site-directed mutagenesis of ActA, immunofluorescence with anti-p21-Arc antibody, latrunculin B treatment Journal of cell science Medium 10954425
2005 NMR spectroscopy using site-directed spin labeling and methyl-TROSY, combined with chemical cross-linking, identified that the extreme C-terminus of the A region and the C-terminus of the C region of N-WASP VCA are proximal to ARPC3 in the Arp2/3 complex, implicating ARPC3 in the binding interface for activating NPFs. Site-directed spin labeling, methyl-TROSY NMR, chemical cross-linking/MS Biochemistry High 16285728
2006 Tiam1 (a Rac-GEF) interacts directly with the p21-Arc (ARPC3) subunit of the Arp2/3 complex via its N-terminal pleckstrin homology domain and adjacent coiled-coil region; this interaction co-localizes Tiam1 with the Arp2/3 complex at sites of actin polymerization, and deletion of the p21-Arc-binding domain in Tiam1 impairs its subcellular localization and capacity to activate Rac1. Yeast two-hybrid screening, co-immunoprecipitation, biochemical pulldown, immunofluorescence co-localization, deletion mutagenesis The Biochemical journal High 16599904
2006 Homozygous disruption of the Arpc3 gene (Sleeping Beauty transposon insertion) in mice results in embryonic lethality at the blastocyst stage; in vitro culture shows severe trophoblast spreading impairment with loss of actin-rich structures and absence of mesh-like (Y-branched) actin at the cell periphery, demonstrating that ARPC3 is required for Y-branch actin formation and trophoblast outgrowth. Transposon insertional mutagenesis, conventional gene targeting, in vitro blastocyst culture, immunofluorescence, electron microscopy Molecular and cellular biology High 16880528
2010 Molecular dynamics simulations of the inactive Arp2/3 crystal structure showed that ARPC3 and the globular domains of ARPC2 form one structural block while Arp2, ARPC1, ARPC4 globular domain, and ARPC5 form another; the activation conformational change involves rotation of the second block around an ARPC4 alpha-helix pivot, with ARPC3 remaining in its block throughout the transition. Atomistic molecular dynamics simulation based on crystal structure Biophysical journal Low 20959098
2011 RNAi-mediated knockdown of Arpc3 (and Arpc2) in mouse oocytes disrupted asymmetric division, spindle migration, formation of the actin cap and cortical granule-free domain, and completion of cytokinesis during meiotic maturation, establishing ARPC3 as required for oocyte polarization and asymmetric cell division. siRNA microinjection, immunofluorescence, live imaging in mouse oocytes; pharmacological inhibition with CK666 PloS one Medium 21494665
2011 miR-29a/b directly target the 3'UTR of Arpc3 mRNA in hippocampal neurons; overexpression of miR-29a/b reduces Arpc3 protein levels and converts mushroom-shaped dendritic spines to filopodial-like protrusions, demonstrating that ARPC3 is a downstream effector of miR-29a/b in regulating actin network branching and spine morphology. Luciferase reporter assay (3'UTR targeting), Western blotting, in vitro imaging of dendritic spines in hippocampal neurons The Journal of cell biology High 21930776
2011 S. pombe arc3 (ortholog of human ARPC3) is essential for viability; depletion causes dispersal of F-actin patches with reduced mobility and inhibits endocytosis. Human ARPC3 complemented the viability defect of the arc3 null mutant and localized to F-actin patches, demonstrating functional conservation. Gene deletion, conditional expression repression, F-actin imaging, endocytosis assay, cross-species complementation Yeast (Chichester, England) High 21449051
2012 Nucleation promoting factors JMY and WAVE2 act upstream of the Arp2/3 complex in mouse oocytes; knockdown of Arpc2 or Arpc3 did not affect JMY or WAVE2 expression or localization, establishing that NPFs are upstream regulators of ARPC3-containing Arp2/3 complex during oocyte asymmetric division. siRNA knockdown of Arpc2 and Arpc3, immunofluorescence, Western blotting in mouse oocytes PloS one Medium 23272233
2012 Fibroblasts derived from ARPC3-/- mouse embryonic stem cells lack lamellipodia but form filopodia-like protrusions with formins (mDia1/mDia2) at their tips; these cells show normal migration speed but a strong defect in persistent directional migration due to uncoordinated leading-edge protrusion. Genetic knockout (isogenic ES cell differentiation), live-cell imaging, immunofluorescence, migration assays The Journal of cell biology High 22492726
2013 Conditional postnatal knockout of ArpC3 in forebrain excitatory neurons causes asymmetric structural plasticity of dendritic spines followed by progressive loss of spine synapses, accompanied by cognitive, psychomotor, and social disturbances, demonstrating that ARPC3-dependent actin polymerization is required for maintaining dendritic spine structure and synaptic function. Conditional knockout mouse (Cre-lox), electron microscopy, immunofluorescence, behavioral testing The Journal of neuroscience : the official journal of the Society for Neuroscience High 23554489
2013 Loss of ArpC3 in the epidermis results in defective tight junction assembly/function and impaired terminal differentiation into cornified envelopes; YAP was found to be inappropriately active in ArpC3-mutant tissue, and YAP inhibition rescued both differentiation and barrier defects, placing ARPC3/Arp2/3 upstream of YAP in epidermal barrier formation. Conditional knockout mouse (epidermis-specific), barrier assays, immunofluorescence, YAP inhibition rescue experiment Proceedings of the National Academy of Sciences of the United States of America High 24043783
2015 In ARPC3-null fibroblasts, formin-family nucleators are required for filopodia-like protrusion extension but are insufficient to produce a continuous leading edge; myosin II activity is required for coordinated advancement of inter-FLP arc regions, explaining the directional migration defect in Arpc3-null cells. Genetic knockout fibroblasts, pharmacological inhibition of formins and myosin II, live-cell imaging, mathematical modeling with experimental verification Molecular biology of the cell High 25568333
2015 Loss of ArpC3 in intestinal enterocytes disrupts the endolysosomal system organization without affecting cortical F-actin or cell polarity; this impairs transcytosis of IgG and lipid absorption, demonstrating that the Arp2/3 complex's role in vesicle trafficking rather than cortical actin underlies intestinal phenotypes. Intestine-specific conditional knockout mouse, subcellular fractionation, transcytosis assay, lipid absorption assay, immunofluorescence Molecular biology of the cell High 25833710
2014 Knockdown of ARPC3 (or Arp3) strongly impaired adipocyte differentiation; Arp2/3 complex is required for the assembly of cortical F-actin structures at the plasma membrane during adipogenesis, which in turn are essential for GLUT4 vesicle exocytosis and insulin signal transduction. siRNA knockdown, F-actin imaging, GLUT4 exocytosis assay, insulin signaling assay in differentiating adipocytes The Biochemical journal Medium 25220164
2020 Cryo-electron tomography of branch junctions in cells at 9 Å resolution revealed a new set of interactions between the Arp2/3 complex and the mother actin filament; ARPC3 plays a central structural role in stabilizing the active conformation of the complex within the branch junction. Cryo-electron tomography, subtomogram averaging (9.0 Å resolution), in-cell structure determination Nature communications High 33353942
2020 Loss or inhibition of mTOR in oligodendrocyte precursor cells reduced ARPC3 expression along with profilin2, and elevated active cofilin, resulting in defective actin polymerization, reduced cellular branching, and delayed myelination initiation, placing ARPC3 downstream of mTOR in oligodendrocyte differentiation. Conditional knockout of mTOR in oligodendrocytes, Western blotting, phalloidin staining, morphological analysis The Journal of neuroscience : the official journal of the Society for Neuroscience Medium 32139584
2020 Circuit-selective knockout of ArpC3 in prefrontal cortical neurons projecting to the basolateral amygdala elevates neuronal excitability, disrupts socially evoked neural activity, and causes abnormal social behavior; optogenetic silencing of this circuit rescues the behavioral deficit, establishing a gene-to-circuit mechanism. Circuit-selective conditional knockout (Cre-lox with retrograde viral vectors), in vivo electrophysiology, optogenetics, behavioral assays Cell reports High 32726629
2017 ARPC3 interacts with IFT20 (an intraflagellar transport component) in T cells; RNAi depletion of ARPC3 impairs TCR accumulation and phosphotyrosine signaling at the immune synapse due to failure of endosomal TCR polarization, despite correct centrosome translocation. Quantitative mass spectrometry interactome, RNAi knockdown, confocal imaging of immune synapses in antigen-specific T cell conjugates Journal of cell science Medium 28154159
2019 miR-124-5p directly targets the 3'UTR of ARPC3 (and ARPC4) mRNA, as confirmed by luciferase reporter assay; transfection of miR-124-5p mimics reduces ARPC3 and ARPC4 protein levels and suppresses phagocytosis in THP-1 macrophages and primary human macrophages. Luciferase reporter assay, Western blotting, transfection of miRNA mimics, phagocytosis assay Frontiers in immunology High 31636629
2023 Nuclear cGAS induced by VEGF-A stimulation regulates ARPC3 expression via a miR-212-5p-ARPC3 cascade; this modulates VEGF-A-mediated angiogenesis through cytoskeletal dynamics and VEGFR2 trafficking from the trans-Golgi network to the plasma membrane. Nuclear fractionation, overexpression/knockdown of cGAS, miRNA mimics/inhibitors, VEGFR2 trafficking assay, in vitro and in vivo angiogenesis assays Cell reports Medium 37027305
2024 Genetic depletion of ArpC3 in alveolar type 2 cells (AT2s) inhibits AT2 migration between alveolar units following lung injury, leading to impaired regeneration of both AT2 and AT1 cells in vivo, establishing ARPC3-dependent migration as required for lung stem cell-mediated repair. Conditional genetic knockout, longitudinal intravital imaging, in vivo lung injury and regeneration assays Developmental cell High 38377991
2025 ARPC3 is identified as a direct target of miR-26a-5p in extravillous trophoblasts (EVTs); ARPC3 knockdown mimics miR-26a-5p overexpression by disrupting actin cytoskeletal organization (converting lamellipodia to filopodia at leading edges) and impairing EVT invasion and migration. Ectopic ARPC3 expression rescues the invasion/migration defects caused by miR-26a-5p overexpression. miRNA mimic/inhibitor transfection, ARPC3 knockdown, ARPC3 overexpression rescue, immunofluorescence of F-actin, Transwell invasion assay, placental explant outgrowth assay Biology of reproduction High 40626921
2022 The cytoplasmic tail motif V1264L1265 of the SARS-CoV-2 spike protein interacts with ARPC3; reducing ARPC3 expression significantly repressed live SARS-CoV-2 virion assembly, identifying ARPC3 as a host factor required for viral assembly. Co-immunoprecipitation, siRNA knockdown of ARPC3, live SARS-CoV-2 and pseudovirus assembly assays Antiviral research Medium 36572190
2016 Chemical cross-linking/mass spectrometry identified direct protein-protein interactions between PKD2 (protein kinase D2) and all seven subunits of the Arp2/3 complex including ARPC3, in both cytosolic and Golgi-enriched fractions, suggesting PKD2 interacts with ARPC3-containing Arp2/3 at the trans-Golgi network. Affinity enrichment, chemical cross-linking, mass spectrometry Journal of proteome research Medium 27559607
2025 CKI (and its active component matrine) inhibit breast cancer lung metastasis by upregulating MTSS1 and downregulating ARPC3 expression, altering F-actin structure, and inhibiting EMT; ARPC3 is placed downstream of MTSS1 in a pathway regulating actin-based cancer cell invasion. Proteomic analysis, Western blotting, immunohistochemistry, immunofluorescence of F-actin, Transwell invasion assay, in vivo tumor/metastasis model Journal of ethnopharmacology Medium 40446975

Source papers

Stage 0 corpus · 86 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Identification of essential genes in cultured mammalian cells using small interfering RNAs. Journal of cell science 703 11792820
1997 The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. The Journal of cell biology 430 9230079
2001 Crystal structure of Arp2/3 complex. Science (New York, N.Y.) 414 11721045
2012 The Arp2/3 complex is required for lamellipodia extension and directional fibroblast cell migration. The Journal of cell biology 282 22492726
2011 Arp2/3 complex regulates asymmetric division and cytokinesis in mouse oocytes. PloS one 143 21494665
2013 Disruption of Arp2/3 results in asymmetric structural plasticity of dendritic spines and progressive synaptic and behavioral abnormalities. The Journal of neuroscience : the official journal of the Society for Neuroscience 142 23554489
2011 Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology. The Journal of cell biology 119 21930776
2006 SRY-box containing gene 11 (Sox11) transcription factor is required for neuron survival and neurite growth. Neuroscience 115 17055661
2013 Phlegm-dampness constitution: genomics, susceptibility, adjustment and treatment with traditional Chinese medicine. The American journal of Chinese medicine 78 23548117
2015 SCARN a Novel Class of SCAR Protein That Is Required for Root-Hair Infection during Legume Nodulation. PLoS genetics 68 26517270
2013 Actin-related protein2/3 complex regulates tight junctions and terminal differentiation to promote epidermal barrier formation. Proceedings of the National Academy of Sciences of the United States of America 68 24043783
2020 Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. Nature communications 62 33353942
2009 Detection and expression of the phosphonate transporter gene phnD in marine and freshwater picocyanobacteria. Environmental microbiology 57 19220397
2013 Silencing of the ARP2/3 complex disturbs pancreatic cancer cell migration. Anticancer research 56 23267127
2006 Interaction between Tiam1 and the Arp2/3 complex links activation of Rac to actin polymerization. The Biochemical journal 47 16599904
2000 Mutations of arginine residues within the 146-KKRRK-150 motif of the ActA protein of Listeria monocytogenes abolish intracellular motility by interfering with the recruitment of the Arp2/3 complex. Journal of cell science 46 10954425
2015 The Arp2/3 complex has essential roles in vesicle trafficking and transcytosis in the mammalian small intestine. Molecular biology of the cell 44 25833710
2006 Sleeping beauty transposon-based phenotypic analysis of mice: lack of Arpc3 results in defective trophoblast outgrowth. Molecular and cellular biology 44 16880528
2015 A mechanism of leading-edge protrusion in the absence of Arp2/3 complex. Molecular biology of the cell 43 25568333
1992 Hemophilia A due to mutations that create new N-glycosylation sites. Proceedings of the National Academy of Sciences of the United States of America 43 1594597
2020 Mechanistic Target of Rapamycin Regulates the Oligodendrocyte Cytoskeleton during Myelination. The Journal of neuroscience : the official journal of the Society for Neuroscience 38 32139584
2005 NMR analyses of the activation of the Arp2/3 complex by neuronal Wiskott-Aldrich syndrome protein. Biochemistry 35 16285728
2020 Dysregulation of the Synaptic Cytoskeleton in the PFC Drives Neural Circuit Pathology, Leading to Social Dysfunction. Cell reports 30 32726629
2000 Recruitment of the Arp2/3 complex and mena for the stimulation of actin polymerization in growth cones by nerve growth factor. Journal of neuroscience research 29 10797548
2020 Exosomes Derived from the Human Primary Colorectal Cancer Cell Line SW480 Orchestrate Fibroblast-Led Cancer Invasion. Proteomics 28 32438511
2014 Arp2/3 complex regulates adipogenesis by controlling cortical actin remodelling. The Biochemical journal 26 25220164
2017 Identification of salivary protein biomarkers for orthodontically induced inflammatory root resorption. Proteomics. Clinical applications 25 28371361
2010 Antiparkinsonian trophic action of glial cell line-derived neurotrophic factor and transforming growth factor β1 is enhanced after co-infusion in rats. Experimental neurology 25 20713051
2017 The T cell IFT20 interactome reveals new players in immune synapse assembly. Journal of cell science 24 28154159
2010 Molecular dynamics simulations of Arp2/3 complex activation. Biophysical journal 24 20959098
2006 Proteomic signatures in laryngeal squamous cell carcinoma. ORL; journal for oto-rhino-laryngology and its related specialties 24 17127822
2019 mir-124-5p Regulates Phagocytosis of Human Macrophages by Targeting the Actin Cytoskeleton via the ARP2/3 Complex. Frontiers in immunology 22 31636629
2016 Identification of reliable reference genes for qRT-PCR studies of the developing mouse mammary gland. Scientific reports 21 27752147
2023 Nuclear translocation of cGAS orchestrates VEGF-A-mediated angiogenesis. Cell reports 20 37027305
2021 Biallelic truncating variants in ATP9A cause a novel neurodevelopmental disorder involving postnatal microcephaly and failure to thrive. Journal of medical genetics 20 34379057
2018 Glycerol monolaurate induces filopodia formation by disrupting the association between LAT and SLP-76 microclusters. Science signaling 20 29717064
2022 Aging-Associated Changes in Oxidative Stress Negatively Impacts the Urinary Bladder Urothelium. International neurourology journal 19 35793989
2024 Stem cell migration drives lung repair in living mice. Developmental cell 18 38377991
2019 The tomato Arp2/3 complex is required for resistance to the powdery mildew fungus Oidium neolycopersici. Plant, cell & environment 17 31038756
2017 TaARPC3, Contributes to Wheat Resistance against the Stripe Rust Fungus. Frontiers in plant science 17 28769954
2008 Molecular dynamics simulation and coarse-grained analysis of the Arp2/3 complex. Biophysical journal 17 18805923
2016 Lecithin Prevents Cortical Cytoskeleton Reorganization in Rat Soleus Muscle Fibers under Short-Term Gravitational Disuse. PloS one 16 27073851
2005 Two closely linked variations in actin cytoskeleton pathway in a Chinese pedigree with disseminated superficial actinic porokeratosis. Journal of the American Academy of Dermatology 16 15928614
2001 Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub. Biochemical and biophysical research communications 16 11162547
2015 Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study. Lipids in health and disease 14 25971815
2014 Effects of acute and repeated cocaine on markers for neural plasticity within the mesolimbic system in rats. Psychopharmacology 14 24912888
2013 CaCO3 biomineralization on cyanobacterial surfaces: insights from experiments with three Synechococcus strains. Colloids and surfaces. B, Biointerfaces 14 23899673
2011 In silico analysis of combinatorial microRNA activity reveals target genes and pathways associated with breast cancer metastasis. Cancer informatics 14 21552493
2014 Novel identification of Dermacentor variabilis Arp2/3 complex and its role in rickettsial infection of the arthropod vector. PloS one 13 24733187
2012 Nucleation promoting factors regulate the expression and localization of Arp2/3 complex during meiosis of mouse oocytes. PloS one 13 23272233
2011 Schizosaccharomyces pombe Arc3 is a conserved subunit of the Arp2/3 complex required for polarity, actin organization, and endocytosis. Yeast (Chichester, England) 13 21449051
2025 Rare disease gene association discovery in the 100,000 Genomes Project. Nature 11 40011789
2018 Transcriptome analysis of adherens junction pathway-related genes after peripheral nerve injury. Neural regeneration research 11 30136696
2016 Protein Interaction Network of Human Protein Kinase D2 Revealed by Chemical Cross-Linking/Mass Spectrometry. Journal of proteome research 11 27559607
2022 Expression of actin- and oxidative phosphorylation-related transcripts across the cortical visuospatial working memory network in unaffected comparison and schizophrenia subjects. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 10 35034100
2022 Effects of lipoteichoic and arachidonic acids on the immune-regulatory mechanism of bovine mammary epithelial cells using multi-omics analysis. Frontiers in veterinary science 10 36090174
2016 A CpG-SNP Located within the ARPC3 Gene Promoter Is Associated with Hypertriglyceridemia in Severely Obese Patients. Annals of nutrition & metabolism 10 27055012
2023 Integrative analysis of genes reveals endoplasmic reticulum stress-related immune responses involved in dilated cardiomyopathy with fibrosis. Apoptosis : an international journal on programmed cell death 8 37462883
2022 A new intracellular targeting motif in the cytoplasmic tail of the spike protein may act as a target to inhibit SARS-CoV-2 assembly. Antiviral research 8 36572190
2016 The role of viral protein Ac34 in nuclear relocation of subunits of the actin-related protein 2/3 complex. Virologica Sinica 8 27900558
2024 Design, Synthesis, Characterization, and Cytotoxicity of New Pyrazolylmethylene-2-thioxoimidazolidin-4-one Derivatives towards Androgen-Sensitive LNCaP Prostate Cancer Cells. Biomolecules 6 39062524
2024 Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model. Biomolecules 6 39199289
2025 Cytochalasin B Mitigates the Inflammatory Response in Lipopolysaccharide-Induced Mastitis by Suppressing Both the ARPC3/ARPC4-Dependent Cytoskeletal Changes and the Association Between HSP70 and the NLRP3 Inflammasome. International journal of molecular sciences 4 40243637
2024 Combining single-cell RNA sequencing and network pharmacology to explore the target of cangfu daotan decoction in the treatment of obese polycystic ovary syndrome from an immune perspective. Frontiers in pharmacology 4 39539629
2023 iTRAQ and PRM-Based Comparative Proteomic Profiling of the Hippocampus in Rat Models of Epilepsy. Journal of integrative neuroscience 4 36722232
2022 Raloxifene Suppresses Tumor Growth and Metastasis in an Orthotopic Model of Castration-Resistant Prostate Cancer. Biomedicines 4 35453603
2022 Analysis of the multi-physiological and functional mechanism of wheat alkylresorcinols based on reverse molecular docking and network pharmacology. Food & function 4 35943408
2018 Frazzled can act through distinct molecular pathways in epithelial cells to regulate motility, apical constriction, and localisation of E-Cadherin. PloS one 4 29518139
2009 Reassessment of microarray expression data of porokeratosis by quantitative real-time polymerase chain reaction. Journal of cutaneous pathology 4 19615027
2025 Single-cell hdWGCNA reveals a novel diagnostic model and signature genes of macrophages associated with chronic obstructive pulmonary disease. Inflammation research : official journal of the European Histamine Research Society ... [et al.] 3 40244418
2019 Expression of progesterone receptor, estrogen receptors α and β, and kisspeptin in the hypothalamus during perinatal development of gonad-lacking steroidogenic factor-1 knockout mice. Brain research 3 30776325
2016 CONTENT OF CYTOSKELETON PROTEINS IN OOCYTES OF THIRD GENERATION DROSOPHILA MELANOGASTER FRUIT FLIES OBTAINED AFTER A 44.5-DAY SPACE FLIGHT. Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine 3 29873978
2002 Characterization of Arp2/3 complex in chicken tissues. Cell structure and function 3 12502893
2025 Compound kushen injection inhibits breast cancer lung metastasis through regulating MTSS1/ARPC3/F-actin. Journal of ethnopharmacology 2 40446975
2025 Salivary and gingival crevicular fluid (GCF) protein biomarkers as proxies of root resorption following orthodontic tooth movement. A systematic review. Journal of clinical and experimental dentistry 2 40485969
2026 Integrated single-cell and spatial transcriptomics combined with whole-exome sequencing reveal key hub genes and epithelial heterogeneity in bladder cancer. Frontiers in oncology 0 41669266
2026 Cell type resolved MR based on brain single cell eQTLs corroborated by single cell RNA sequencing uncovers neuroimmune and vascular programs in intracerebral hemorrhage. Journal of translational medicine 0 41673661
2026 QTL mapping, validation, and candidate gene predicting for maize plant architecture traits via bulked segregant analysis plus linkage analysis with F₂ population. BMC plant biology 0 41981466
2026 [Impact of house dust mite subcutaneous immunotherapy on plasma protein profiles in children with asthma]. Zhonghua yi xue za zhi 0 42049523
2026 An engineered streptavidin condensate platform for chemically inducible control of endogenous proteins in mammalian cells. bioRxiv : the preprint server for biology 0 42244538
2025 Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis. Biomolecules 0 40305275
2025 Liangxue Qushi Zhiyang Decoction Inhibits Atopic Dermatitis in Mice via FcγR-Mediated Phagocytosis. Mediators of inflammation 0 40322063
2025 Exosomal Gene Biomarkers in Osteosarcoma: Mifepristone as a Targeted Therapeutic Revealed by Multi-Omics Analysis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 0 40616392
2025 Human extravillous trophoblast invasion and migration are impeded by excessive miR-26a-5p: dysregulated actin cytoskeleton in extravillous trophoblasts during early-onset severe preeclampsia†. Biology of reproduction 0 40626921
2022 Application of iTRAQ Technology to Identify Differentially Expressed Proteins of Sauce Lamb Tripe with Different Secondary Pasteurization Treatments. Foods (Basel, Switzerland) 0 35454754
2015 Construction of a full-length cDNA library and preliminary analysis of expressed sequence tags from lymphocytes of half-pipe snowboarding athletes. Genetics and molecular research : GMR 0 26505445

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