Affinage

ARPC4

Actin-related protein 2/3 complex subunit 4 · UniProt P59998

Length
168 aa
Mass
19.7 kDa
Annotated
2026-06-09
52 papers in source corpus 25 papers cited in narrative 26 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ARPC4 (p20-Arc) is a core structural subunit of the seven-protein Arp2/3 complex, the actin nucleator that builds branched filament networks at sites of dynamic polymerization such as lamellipodia and bacterial actin tails (PMID:9230079). Together with ARPC2, ARPC4 forms the structural core of the complex through long C-terminal alpha helices and similarly folded N-terminal alpha/beta domains (PMID:11721045), and it serves as a subunit-interaction hub contacting ARPC2, ARPC3, and ARPC5 (PMID:11162547). The ARPC2–ARPC4 interface directly engages the mother actin filament: residues at this surface are required for actin nucleation, Y-branch formation, high-affinity F-actin binding, and branch stability (PMID:20404198, PMID:18381280), and the ARPC1–ARPC4 contact is required to propagate the WASp activation signal (PMID:20071330). Activation involves global conformational reorganization transmitted to ARPC4 upon ATP and WASp-VCA binding (PMID:19298826), with auto-inhibitory salt bridges at the Arp2–Arp3–ARPC4 interface gating the complex; their disruption yields constitutive nucleation (PMID:22125478). ARPC4 activity is regulated post-translationally: UFL1, when phosphorylated by Akt, UFMylates ArpC4 to promote lamellipodia formation, migration, and metastasis (PMID:40419786), whereas clostridial binary toxins ADP-ribosylate ARPC4 to inhibit nucleation and collapse F-actin networks (PMID:36429089). Through this branched-actin function, ARPC4 supports a broad range of cellular and tissue processes — epidermal homeostasis with Nrf2 restraint (PMID:29113991), pancreatic acinar-to-ductal metaplasia downstream of mTORC1/2–Rac1 signaling (PMID:33388318), intestinal epithelial barrier integrity under mechanical stress (PMID:40930096), microglial maturation (PMID:41760937), and cancer cell migration and invasion across tumor types (PMID:23267127, PMID:31190401, PMID:41793310). A recurrent de novo missense variant (p.Arg158Cys) causes reduced cellular F-actin in patients with microcephaly and speech delay, linking ARPC4 to a neurodevelopmental disorder (PMID:35047857).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1997 High

    Established that ARPC4 is a bona fide subunit of the human Arp2/3 complex and that the complex localizes to sites of dynamic actin assembly, defining its cellular arena.

    Evidence Protein purification, sequencing, and immunofluorescence in fibroblasts and Listeria actin tails

    PMID:9230079

    Open questions at the time
    • Did not resolve ARPC4's specific structural or functional contribution within the complex
    • Localization correlative, not mechanistic
  2. 2001 High

    Resolved how ARPC4 is built into the complex, showing it pairs with ARPC2 to form the structural core and acts as a hub for inter-subunit contacts.

    Evidence 2.0 Å X-ray crystallography of bovine complex and pairwise yeast two-hybrid mapping

    PMID:11162547 PMID:11721045

    Open questions at the time
    • Static structure does not reveal the activation conformational change
    • Y2H interactions not validated by reciprocal biochemistry
  3. 2010 High

    Defined ARPC4 functionally by mapping the ARPC2–ARPC4 actin-filament-binding interface and showing distinct residues control nucleation, branching, F-actin affinity, and branch stability.

    Evidence Molecular dynamics/docking with mutagenesis and in vitro actin assembly and F-actin binding assays

    PMID:20404198

    Open questions at the time
    • Interface defined computationally before high-resolution branch junction structures
    • Did not address the activating conformational rotation
  4. 2011 Medium

    Connected ARPC4 to activation by identifying an auto-inhibitory salt-bridge network at the Arp2–Arp3–ARPC4 interface whose disruption produces NPF-independent nucleation.

    Evidence Molecular dynamics simulations plus biochemical assays of a gain-of-function ARPC4 mutant complex; complemented by H/DX-MS showing allosteric conformational transmission to ARPC4 and the ARPC4 pivot-helix rotation model

    PMID:19298826 PMID:20959098 PMID:22125478

    Open questions at the time
    • Pivot-helix rotation rests on simulation without direct experimental validation
    • In vivo relevance of the Arp2-phosphorylation gating not established
  5. 2008 High

    Demonstrated in a genetically tractable system that the ARPC2–ARPC4 and ARPC1–ARPC4 contacts are required for nucleation and WASp activation independent of complex assembly.

    Evidence Systematic mutagenesis in S. cerevisiae with purified mutant complexes, in vitro nucleation assays, and endocytosis assays

    PMID:18381280 PMID:20071330

    Open questions at the time
    • Yeast residue requirements not directly mapped onto human ARPC4 function
    • Mechanism of signal propagation through the interface not resolved at atomic level
  6. 2017 High

    Established a tissue-level requirement for ARPC4 in epidermal homeostasis and linked actin loss to a transcriptional consequence via Nrf2.

    Evidence Conditional Arpc4 KO mouse, keratinocyte KO, Arp2/3 inhibition, and in vitro Nrf2–F-actin binding assay

    PMID:29113991

    Open questions at the time
    • Whether Nrf2 sequestration by F-actin is the sole driver of the phenotype unclear
    • Direct ARPC4 contribution versus whole-complex depletion not separated
  7. 2021 High

    Positioned ARPC4-containing Arp2/3 as a convergent effector of mTORC1/2–Rac1 signaling driving Kras-induced pancreatic metaplasia, and linked an ARPC4 missense variant to a neurodevelopmental disorder.

    Evidence Conditional Arpc4 KO mouse with Rptor/Rictor epistasis and ADM assays; patient-cell F-actin quantification for the p.Arg158Cys variant

    PMID:33388318 PMID:35047857

    Open questions at the time
    • Direct biochemical effect of p.Arg158Cys on complex assembly/nucleation not reconstituted
    • How mTOR signals couple to ARPC4 mechanistically beyond Rac1 not detailed
  8. 2022 Medium

    Identified ARPC4 as a post-translationally regulated subunit, being ADP-ribosylated by clostridial binary toxins to inhibit nucleation.

    Evidence Mass spectrometry site identification, in vitro Arp2/3 activity assays, and cell/explant imaging

    PMID:36429089

    Open questions at the time
    • Relative contribution of ARPC4 versus actin/Arp2 ADP-ribosylation to inhibition unresolved
    • Physiological host modifications at these sites not addressed
  9. 2025 High

    Defined an activating regulatory axis in which Akt-phosphorylated UFL1 UFMylates ArpC4 to drive lamellipodia formation and metastatic migration.

    Evidence Co-IP, in vitro UFMylation, phosphorylation mapping (Akt T426 on UFL1), and loss-of-function migration/invasion/lamellipodia assays

    PMID:40419786

    Open questions at the time
    • UFMylated residue effect on complex conformation/nucleation not structurally defined
    • In vivo metastasis dependence on this modification in physiological settings not established
  10. 2025 High

    Showed ARPC4 is required for mechanically stressed epithelial barrier integrity, revealing that branched-actin defects manifest only under load.

    Evidence Inducible gut-specific Arpc4 KO mouse, ex vivo intestinal slices, and organoids with defined mechanical challenge and tight junction imaging

    PMID:40930096

    Open questions at the time
    • Molecular link between branched actin and tight junction maintenance not resolved
    • Actomyosin contractility interplay mechanistically incomplete
  11. 2026 Medium

    Extended ARPC4's roles to immune and CNS cell maturation and to nuclear envelope integrity, while a controlled study showed an apparent DNA-repair role is indirect.

    Evidence Conditional Arpc4 KO mice in microglia and Langerhans cells with morphological/transcriptomic/nuclear assays; CRISPR KO in PDAC lines with collagen invasion and β1 integrin analysis; siRNA HDR reporter with cell-cycle controls

    PMID:41760937 PMID:41793310 PMID:41963733

    Open questions at the time
    • Langerhans cell nuclear-integrity finding remains a preprint without peer review
    • How branched actin maintains nuclear envelope integrity mechanistically unknown
    • Direct versus cell-cycle-mediated effects need disentangling across contexts

Open questions

Synthesis pass · forward-looking unresolved questions
  • How distinct post-translational modifications (UFMylation, ADP-ribosylation) and disease variants of ARPC4 alter the conformational activation cycle and branch-forming activity at atomic resolution remains unresolved.
  • No structure of modified or variant ARPC4 within an activated branch junction
  • Tissue-specificity of ARPC4 requirements not mechanistically explained beyond whole-complex depletion

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005856 cytoskeleton 2 GO:0005829 cytosol 1
Pathway
R-HSA-1643685 Disease 3 R-HSA-1266738 Developmental Biology 2
Partners
ARPC1ARPC2ARPC3ARPC5UFL1
Complex memberships
Arp2/3 complex

Evidence

Reading pass · 26 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 ARPC4 (p20-Arc) was identified as one of seven subunits of the human Arp2/3 complex; the complex localizes to lamellipodia of fibroblasts and Listeria actin tails (but not actin bundles), consistent with a role in promoting actin assembly at sites of dynamic polymerization. Protein purification, sequencing, immunofluorescence localization in cells The Journal of cell biology High 9230079
2001 Crystal structure of bovine Arp2/3 complex at 2.0 Å resolution revealed that ARPC4 (p20) and ARPC2 (p34) form the core of the complex through long C-terminal alpha helices and similarly folded N-terminal alpha/beta domains. X-ray crystallography at 2.0 Å resolution Science (New York, N.Y.) High 11721045
2001 Yeast two-hybrid analysis showed that p20-Arc (ARPC4) acts as a hub for subunit interactions within the human Arp2/3 complex, interacting with p21-Arc (ARPC3), p34-Arc (ARPC2), and p16-Arc (ARPC5); p41-Arc only interacted with the p20-Arc/p16-Arc heterodimer. Structural integrity was important for p20-Arc/p21-Arc association, while the N-terminal half of p34-Arc was dispensable for its binding to p20-Arc. Yeast two-hybrid assay Biochemical and biophysical research communications Medium 11162547
2010 Molecular dynamics and protein-protein docking simulations, validated by mutagenesis, defined an actin-filament-binding interface on ARPC2 and ARPC4. Residues at this interface are required for actin nucleation, Y-branching, high-affinity F-actin binding, and Y-branch stability, demonstrating that Arp2/3 complex affinity for F-actin independently modulates branch formation and stability. Molecular dynamics/docking simulations + mutagenesis + in vitro actin assembly assays + F-actin binding assays Proceedings of the National Academy of Sciences of the United States of America High 20404198
2010 Molecular dynamics simulations of Arp2/3 activation showed that one structural block (comprising Arp2, ARPC1, the globular domain of ARPC4, and ARPC5) rotates ~30° around a pivot point in an alpha-helix of ARPC4 (Glu81–Asn100) to bring Arp2 into proximity with Arp3 during activation. Atomistic molecular dynamics simulations Biophysical journal Low 20959098
2011 Phosphorylation of Arp2 destabilizes a network of auto-inhibitory salt-bridge interactions at the interface of Arp2, Arp3, and ARPC4, permitting Arp2 reorientation to an activation-competent state. A gain-of-function ARPC4 mutant predicted to disrupt these interactions showed substantial actin nucleation activity in the absence of NPFs. Molecular dynamics simulations + biochemical assays with recombinant Arp2/3 complex carrying ARPC4 mutations PLoS computational biology Medium 22125478
2009 Hydrogen/deuterium exchange mass spectrometry showed that ATP binding to Arp2/3 complex causes conformational rearrangements in Arp2 and Arp3 that are allosterically transmitted to ARPC4 (and ARPC1, ARPC2, ARPC5); WASp VCA binding further modulates exchange rates in ARPC4, indicating global conformational reorganization involving this subunit upon activation. Hydrogen/deuterium exchange coupled with mass spectrometry Journal of molecular biology Medium 19298826
2008 In S. cerevisiae, a contact surface between p35/ARPC2 and p19/ARPC4 was identified as required for actin nucleation and endocytosis; mutations near this interface abolished nucleation without disrupting complex integrity. Systematic mutagenesis in S. cerevisiae + purification of mutant complexes + in vitro actin assembly assays + endocytosis assays The Journal of biological chemistry High 18381280
2010 In S. cerevisiae, lethal mutations at the p40/ARPC1 contact with p19/ARPC4 specifically impaired WASp-induced nucleation of purified Arp2/3 complex, placing ARPC4 at the interface required for WASp activation signal propagation. Mutagenesis of ARPC1 + purification of mutant complexes + in vitro actin nucleation assays The Journal of biological chemistry High 20071330
2017 Conditional knockout of Arpc4 in mouse epidermis depleted the Arp2/3 complex and caused a psoriasis-like disease; Arpc4 knockout in cultured keratinocytes was sufficient to induce nuclear accumulation of Nrf2, upregulation of Nrf2 target genes, and decreased F-actin levels. In vitro, Nrf2 was shown to bind to filamentous actin. Conditional Arpc4 knockout mouse model + keratinocyte culture KO + pharmacological Arp2/3 inhibition + in vitro F-actin binding assay Development (Cambridge, England) High 29113991
2021 Conditional ablation of Arpc4 in mouse pancreatic acinar cells demonstrated that the Arp2/3 complex is required for KrasG12D-driven acinar-to-ductal metaplasia (ADM) in vivo; mTORC1 regulates Arp2/3 complex activity via Rac1/Arp3 translation while mTORC2 promotes Arp2/3 via Akt/Rac1, converging on ARPC4-containing Arp2/3 as a common downstream effector for actin cortex remodeling and ADM. Conditional Arpc4 knockout mouse + in vitro ADM assays + epistasis with Rptor/Rictor conditional knockouts Gastroenterology High 33388318
2021 A recurrent de novo missense variant in ARPC4 (p.Arg158Cys) found in patients with microcephaly and speech delay was associated with decreased F-actin levels in cells from affected individuals, implicating ARPC4 in actin filament network formation required for neurodevelopment. Patient cell-based F-actin quantification (immunofluorescence/imaging) in cells from affected individuals carrying the variant HGG advances Medium 35047857
2025 UFL1 (UFM1-specific E3 ligase 1) interacts with ArpC4 and catalyzes its UFMylation. Akt phosphorylates UFL1 at T426, which enhances UFL1's interaction with ArpC4 and promotes ArpC4 UFMylation, thereby facilitating lamellipodia formation, cell migration, invasion, and metastasis. Co-immunoprecipitation, in vitro UFMylation assay, phosphorylation mapping, loss-of-function cell migration/invasion assays, lamellipodia imaging Nature structural & molecular biology High 40419786
2022 Binary clostridial toxins (CDT, C2I, Iota) ADP-ribosylate ARPC4/5 (among other Arp2/3 subunits) in addition to actin and Arp2, and this modification inhibits Arp2/3 complex actin-nucleating activity, causing collapse of lamellipodia and F-actin networks in cells. Mass spectrometry identification of ADP-ribosylation sites + in vitro Arp2/3 activity assays + cell imaging (Caco2 cells + mouse colon explants) Cells Medium 36429089
2013 siRNA-mediated silencing of ARPC4 significantly reduced cell migration (50–68% decrease) in pancreatic cancer cell lines without affecting other processes, indicating ARPC4 is a key functional subunit for Arp2/3-dependent migration in these cells. siRNA knockdown + transwell migration assay in multiple pancreatic cancer cell lines Anticancer research Medium 23267127
2019 ARPC4 knockdown in T24 bladder cancer cells attenuated migration, invasion, and pseudopodia formation and disrupted actin cytoskeleton structure, demonstrating a direct role of ARPC4 in actin-dependent invasive behavior. siRNA knockdown + transwell invasion/migration assay + wound-healing assay + immunofluorescence of actin cytoskeleton Journal of cellular biochemistry Medium 31190401
2016 Chemical cross-linking/mass spectrometry identified the entire seven-subunit Arp2/3 complex (including ARPC4) as an interaction partner of human PKD2 in both cytosolic and Golgi-enriched fractions, with evidence of a direct protein-protein interaction between PKD2 and Arp2/3. Affinity enrichment + chemical cross-linking + mass spectrometry Journal of proteome research Low 27559607
2013 Expression of ARPC4 in Mycobacterium tuberculosis severely impaired bacterial growth (evidenced by TEM showing outer-coat shedding), enhanced bacterial clearance in infected macrophages, impaired phagosome-to-lysosome translocation, and suppressed pro-inflammatory cytokine responses. ARPC4 was shown to interact with the essential mycobacterial secretory protein Rv1626, downregulating its expression ~6-fold; Rv1626 also interacted with mammalian Arp2/3 and enhanced actin polymerization. Bacterial expression of ARPC4 + TEM + macrophage infection assays + co-immunoprecipitation (ARPC4–Rv1626 interaction) + real-time PCR PloS one Medium 23894563
2016 Viral protein Ac34 (baculovirus) co-immunoprecipitated with ARPC4 (P20) of Sf9 (insect) cells and induced its nuclear relocation; however, mammalian ARPC4 did not interact with Ac34 and was not relocated, indicating species-specific binding specificity. Immunofluorescence + co-immunoprecipitation Virologica Sinica Low 27900558
2025 In cervical cancer cells, Aurora-A overexpression upregulated ARPC4 expression via activation of the NF-κBp65 signaling pathway (increased NF-κBp65 phosphorylation led to elevated ARPC4 levels), and ARPC4 knockdown antagonized Aurora-A-promoted migration, invasion, and EMT. Plasmid overexpression + shRNA knockdown + NF-κBp65 inhibitor treatment + Western blot + migration/invasion assays Nan fang yi ke da xue xue bao Medium 40294934
2019 A genome-wide CRISPR knockout screen in THP-1 macrophages identified ARPC4 loss-of-function as conferring resistance to Salmonella uptake, placing ARPC4-containing Arp2/3 complex in the actin dynamics pathway required for macrophage phagocytic internalization of bacteria. Genome-wide CRISPR KO screen + validation assays mBio Medium 31594818
2025 In a gut epithelium-specific inducible Arpc4 knockout mouse, loss of Arp2/3 function led to increased intestinal permeability, disrupted tight junction protein localization, epithelial fracturing, and lethality under mechanical challenge; ex vivo organoid experiments showed defects required mechanical stress and elevated actomyosin contractility to manifest. Inducible conditional Arpc4 KO mouse + ex vivo intestinal slice culture + organoid culture + tight junction imaging Current biology : CB High 40930096
2026 Conditional Arpc4 knockout in myeloid cells showed that Arp2/3 complex loss in Langerhans cells leads to cell decline through DNA damage accumulation associated with aberrant nuclear shapes, lamina reduction, and nuclear envelope rupture, revealing a role for Arp2/3/ARPC4 in nuclear envelope integrity and genome maintenance in tissue-resident immune cells. Conditional Arpc4 KO mouse + in vivo LC analysis + in vitro BMDC experiments + cell cycle analysis + nuclear morphology/DNA damage assays bioRxivpreprint Medium
2026 Conditional Arpc4 knockout in microglia showed that Arp2/3 depletion prevents the developmental transition of microglia into ramified cells with homeostatic gene profiles and surveillance function, linking ARPC4-dependent actin branching to microglial maturation in the CNS. Conditional Arpc4 KO mouse + morphological analysis + transcriptomic profiling of microglia + CNS surveillance assays EMBO reports High 41760937
2026 CRISPR/Cas9 knockout of Arpc4 in murine PDAC cell lines downregulated all Arp2/3 complex members and significantly impaired PDAC cell migration, disrupted branched tubular structure formation in collagen I, and inhibited invasive front formation in organoid culture; β1 integrin signaling was identified as a key upstream regulator of Arp2/3-dependent migration through collagen-rich matrices. CRISPR/Cas9 KO + 2D and 3D migration/invasion assays + organoid co-culture + β1 integrin signaling analysis International journal of cancer Medium 41793310
2026 ArpC4 knockdown by siRNA in U2OS cells reduced HDR efficiency, but this effect corresponded with decreased transfection efficiency and reduction in S/G2M cell cycle phases rather than a direct role in DNA repair; WASP/N-WASP were found dispensable for HDR. siRNA knockdown + CRISPR-based HDR reporter assay + cell cycle analysis EMBO reports Medium 41963733

Source papers

Stage 0 corpus · 52 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. The Journal of cell biology 430 9230079
2001 Crystal structure of Arp2/3 complex. Science (New York, N.Y.) 414 11721045
2017 Multicohort analysis reveals baseline transcriptional predictors of influenza vaccination responses. Science immunology 127 28842433
2006 The role of ARPC4 in tip growth and alignment of the polar axis in filaments of Physcomitrella patens. Cell motility and the cytoskeleton 66 16450411
2010 An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. Proceedings of the National Academy of Sciences of the United States of America 63 20404198
2013 Quantitative apical membrane proteomics reveals vasopressin-induced actin dynamics in collecting duct cells. Proceedings of the National Academy of Sciences of the United States of America 60 24085853
2013 Silencing of the ARP2/3 complex disturbs pancreatic cancer cell migration. Anticancer research 56 23267127
2008 BRICK1 is required for apical cell growth in filaments of the moss Physcomitrella patens but not for gametophore morphology. The Plant cell 50 18263777
2019 A Genome-Wide Knockout Screen in Human Macrophages Identified Host Factors Modulating Salmonella Infection. mBio 45 31594818
2017 Knockout of the Arp2/3 complex in epidermis causes a psoriasis-like disease hallmarked by hyperactivation of transcription factor Nrf2. Development (Cambridge, England) 45 29113991
2021 mTORC1 and mTORC2 Converge on the Arp2/3 Complex to Promote KrasG12D-Induced Acinar-to-Ductal Metaplasia and Early Pancreatic Carcinogenesis. Gastroenterology 43 33388318
2008 Functional surfaces on the p35/ARPC2 subunit of Arp2/3 complex required for cell growth, actin nucleation, and endocytosis. The Journal of biological chemistry 25 18381280
2010 The p40/ARPC1 subunit of Arp2/3 complex performs multiple essential roles in WASp-regulated actin nucleation. The Journal of biological chemistry 24 20071330
2010 Molecular dynamics simulations of Arp2/3 complex activation. Biophysical journal 24 20959098
2019 ARPC4 promotes bladder cancer cell invasion and is associated with lymph node metastasis. Journal of cellular biochemistry 23 31190401
2019 Serum Proteomic Signatures of Male Breast Cancer. Cancer genomics & proteomics 22 30850364
2019 mir-124-5p Regulates Phagocytosis of Human Macrophages by Targeting the Actin Cytoskeleton via the ARP2/3 Complex. Frontiers in immunology 22 31636629
2011 Phosphorylation of the Arp2 subunit relieves auto-inhibitory interactions for Arp2/3 complex activation. PLoS computational biology 19 22125478
2008 Molecular dynamics simulation and coarse-grained analysis of the Arp2/3 complex. Biophysical journal 17 18805923
2022 Identifying Novel Osteoarthritis-Associated Genes in Human Cartilage Using a Systematic Meta-Analysis and a Multi-Source Integrated Network. International journal of molecular sciences 16 35457215
2001 Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub. Biochemical and biophysical research communications 16 11162547
2017 Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis. Biochimica et biophysica acta. Proteins and proteomics 15 28627476
2024 Identification of genetic modifiers enhancing B7-H3-targeting CAR T cell therapy against glioblastoma through large-scale CRISPRi screening. Journal of experimental & clinical cancer research : CR 14 38561797
2017 Short interfering RNA-mediated silencing of actin-related protein 2/3 complex subunit 4 inhibits the migration of SW620 human colorectal cancer cells. Oncology letters 14 29435011
2014 Novel identification of Dermacentor variabilis Arp2/3 complex and its role in rickettsial infection of the arthropod vector. PloS one 13 24733187
2009 Nucleotide- and activator-dependent structural and dynamic changes of arp2/3 complex monitored by hydrogen/deuterium exchange and mass spectrometry. Journal of molecular biology 13 19298826
2016 Protein Interaction Network of Human Protein Kinase D2 Revealed by Chemical Cross-Linking/Mass Spectrometry. Journal of proteome research 11 27559607
2022 Expression of actin- and oxidative phosphorylation-related transcripts across the cortical visuospatial working memory network in unaffected comparison and schizophrenia subjects. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 10 35034100
2022 Effects of lipoteichoic and arachidonic acids on the immune-regulatory mechanism of bovine mammary epithelial cells using multi-omics analysis. Frontiers in veterinary science 10 36090174
2013 Expression of the ARPC4 subunit of human Arp2/3 severely affects mycobacterium tuberculosis growth and suppresses immunogenic response in murine macrophages. PloS one 10 23894563
2022 Inhibition of Arp2/3 Complex after ADP-Ribosylation of Arp2 by Binary Clostridioides Toxins. Cells 8 36429089
2021 A recurrent, de novo pathogenic variant in ARPC4 disrupts actin filament formation and causes microcephaly and speech delay. HGG advances 8 35047857
2016 The role of viral protein Ac34 in nuclear relocation of subunits of the actin-related protein 2/3 complex. Virologica Sinica 8 27900558
2025 Akt-phosphorylated UFL1 UFMylates ArpC4 to promote metastasis. Nature structural & molecular biology 7 40419786
2024 Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model. Biomolecules 6 39199289
2022 Dynamic miRNA profile of host T cells during early hepatic stages of Schistosoma japonicum infection. Frontiers in immunology 5 36119054
2016 Comparative Proteomic Profiling of Extracellular Proteins between Normal and Gastric Cancer Cells. Current cancer drug targets 5 26648486
2025 Cytochalasin B Mitigates the Inflammatory Response in Lipopolysaccharide-Induced Mastitis by Suppressing Both the ARPC3/ARPC4-Dependent Cytoskeletal Changes and the Association Between HSP70 and the NLRP3 Inflammasome. International journal of molecular sciences 4 40243637
2025 The Arp2/3 complex maintains gut epithelial integrity under mechanical challenge. Current biology : CB 3 40930096
2022 p53 Related Protein Kinase is Required for Arp2/3-Dependent Actin Dynamics of Hemocytes in Drosophila melanogaster. Frontiers in cell and developmental biology 3 35721516
2002 Characterization of Arp2/3 complex in chicken tissues. Cell structure and function 3 12502893
2025 Actin-myosin complex dissociation initiates programmed cell death during cold storage of grass carp muscle. Food research international (Ottawa, Ont.) 2 40597474
2023 Host-pathogen interaction involving cytoskeleton changes as well as non-coding regulation as primary mechanisms for SRS resistance in Atlantic salmon. Fish & shellfish immunology 2 37004895
2026 The Arp2/3 complex controls the development of homeostatic microglia. EMBO reports 1 41760937
2026 Machine learning framework for mRNA alternative splicing analysis identifies a signature of progression in colorectal adenocarcinoma. Scientific reports 0 41634106
2026 The Rho GTPase signaling pathway modulates Moraxella catarrhalis invasion into human respiratory epithelial cells by regulating actin polymerization. Frontiers in immunology 0 41782863
2026 Arp2/3 complex and β1 integrin drive an invasive front through extracellular matrix adaptation in pancreatic cancer. International journal of cancer 0 41793310
2026 Dispensable players: N-WASP and WASP are not crucial for homology-directed DNA repair. EMBO reports 0 41963733
2026 Discovery of Casdatifan, Part II: A Potent and Orally Bioavailable Inhibitor of Hypoxia Inducible Factor-2α. Journal of medicinal chemistry 0 42246926
2025 [Aurora-A overexpression promotes cervical cancer cell invasion and metastasis by activating the NF-κBp65/ARPC4 signaling axis]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University 0 40294934
2025 Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis. Biomolecules 0 40305275
2025 Integrated proteomics and metabolomics analysis revealed that macrophage-related signals may be potential biomarkers for oral squamous cell carcinoma. Scientific reports 0 41372366

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