Affinage

UFL1

E3 UFM1-protein ligase 1 · UniProt O94874

Length
794 aa
Mass
89.6 kDa
Annotated
2026-06-10
51 papers in source corpus 24 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

UFL1 is the E3 ligase of the UFMylation pathway, covalently conjugating the ubiquitin-like modifier UFM1 onto substrate lysines through a regulated E1→E2→E3 cascade in which its N-terminal helix mediates binding to the E2 enzyme UFC1, competing with the E1 UBA5 in a manner that shifts toward UFL1 once UFC1 is charged with UFM1 (PMID:37988244). A recurring logic of UFL1 substrate UFMylation is the antagonism of ubiquitin-mediated proteasomal degradation: UFL1 stabilizes PD-1 in T cells against ubiquitination (PMID:38377992), stabilizes PARP1 to enhance DNA repair and suppress cGAS-STING activation (PMID:41105513), and stabilizes IFT88 by antagonizing PJA2-mediated ubiquitination to maintain ciliary homeostasis (PMID:41272290), while in other contexts UFMylation destabilizes substrates such as METTL16 and NONO (PMID:41608626, PMID:41184530). In the DNA damage and replication stress response, UFL1 is recruited to double-strand breaks by the MRN complex where it monoufmylates histone H4 to drive ATM activation through a positive feedback loop in which ATM phosphorylates UFL1 at S462 to enhance its ligase activity (PMID:30886146), and it UFMylates PTIP at K148 at stalled forks to assemble the MLL3/4 complex and remodel chromatin (PMID:38649452). UFL1 activity is further tuned by phosphorylation, with AMPK acting on T536 to disable PD-1 UFMylation (PMID:38377992) and Akt acting on T426 to promote ArpC4 UFMylation and lamellipodia-driven migration (PMID:40419786). As an ER membrane-associated protein acting in a complex with CDK5RAP3, UFL1 is essential for ER homeostasis and the unfolded protein response: its loss elevates ER stress and impairs PERK signaling, causing tissue-specific phenotypes including hematopoietic stem cell death (PMID:25952549), cardiomyopathy (PMID:30354401), and myoblast apoptosis through the PERK/eIF2α/ATF4/CHOP axis (PMID:42019645). UFL1 additionally regulates mitotic spindle assembly via RNF20 UFMylation (PMID:42008680) and possesses UFMylation-independent functions, including stabilizing STING by blocking TRIM29 (PMID:35871231).

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 2010 Medium

    Established UFL1's earliest characterized cellular role as an ER membrane protein controlling cell cycle and stability of binding partners, before its UFMylation enzymology was understood.

    Evidence Co-IP, subcellular fractionation, and cell cycle FACS in cultured cells; TAP and in-cell ubiquitination assays

    PMID:20164180 PMID:20531390

    Open questions at the time
    • Did not connect these stabilization roles to UFM1 conjugation activity
    • Mechanism of mutual stabilization with partners not resolved at the enzymatic level
  2. 2015 High

    Genetic ablation showed UFL1 is essential in vivo for cellular homeostasis, defining ER stress/UPR, autophagy, and mitochondrial control as core dependencies.

    Evidence Germline and conditional knockout mice with UPR, autophagy flux, ROS, and p53 pathway analyses in hematopoietic stem cells

    PMID:25952549

    Open questions at the time
    • Direct UFMylation substrates driving the phenotype not identified
    • Did not separate ER stress from autophagy contributions causally
  3. 2018 High

    Tissue-specific knockouts linked UFL1 loss to organ pathology through ER stress signaling, establishing PERK as a key effector arm.

    Evidence Cardiac-specific knockout mice with transcriptomics, ER stress markers, and chemical chaperone rescue; later skeletal muscle KO with PERK inhibitor rescue

    PMID:30354401 PMID:42019645

    Open questions at the time
    • Molecular substrate connecting UFL1 to PERK regulation not defined
    • Whether the effect requires UFM1 conjugation not established
  4. 2019 High

    Identified UFL1 as the chromatin-acting E3 that monoufmylates histone H4 at DNA breaks and revealed a phosphorylation feedback loop with ATM, placing UFMylation upstream of the DNA damage response.

    Evidence Co-IP, in vitro ufmylation assay, S462 mutagenesis, ChIP, and laser micro-irradiation imaging

    PMID:30886146

    Open questions at the time
    • H4 lysine acceptor site not pinpointed in this entry
    • Stoichiometry and turnover of the modification unresolved
  5. 2019 Medium

    Defined CDK5RAP3 as a substrate adaptor for UFL1, clarifying how the ligase achieves substrate specificity in vivo.

    Evidence In vivo Co-IP, conditional knockout mice, and proteomics-based ufmylation profiling in liver

    PMID:30635284

    Open questions at the time
    • Direct substrates recruited via CDK5RAP3 not enumerated
    • Structural basis of adaptor function not addressed
  6. 2023 High

    Solved the structural basis of E2 recognition, showing the UFL1 N-terminal helix binds UFC1 and that UBA5–UFL1 competition for UFC1 coordinates handoff in the cascade.

    Evidence X-ray crystallography of a UFL1–DDRGK1 fusion, NMR, AlphaFold2 modeling, and biochemical binding/mutagenesis assays

    PMID:37988244

    Open questions at the time
    • Structure of UFL1 engaging a substrate not determined
    • How phosphorylation of UFL1 alters this interface not shown
  7. 2024 High

    Demonstrated the dominant UFL1 mechanistic theme of antagonizing substrate ubiquitination via site-specific UFMylation across distinct biological settings (replication forks, immune checkpoints, the ER-mTOR node).

    Evidence In vitro UFMylation assays with lysine mutagenesis (PTIP K148, PD-1), iPOND/fiber assays, conditional knockouts, and complex Co-IP with mTOR/GβL

    PMID:37131258 PMID:38377992 PMID:38649452

    Open questions at the time
    • General rules predicting which substrates are stabilized versus destabilized unclear
    • Crosstalk between UFL1's many substrate-specific roles not integrated
  8. 2024 Medium

    Revealed UFMylation-independent functions of UFL1, broadening its activity beyond UFM1 conjugation.

    Evidence Co-IP, ubiquitination assays with site-specific mutants, and UFMylation/ligase-dead mutant controls for STING regulation; chicken ortholog K63-ubiquitination study

    PMID:35871231 PMID:38477617

    Open questions at the time
    • Mechanism by which UFL1 blocks TRIM29-STING binding not structurally defined
    • Conservation of UFMylation-independent activity to human STING not established in these entries
  9. 2025 High

    Expanded UFL1's substrate repertoire to cytoskeletal, mitotic, ciliary, and metabolic regulators, and showed kinase inputs (Akt T426) gate substrate choice and cancer-relevant phenotypes.

    Evidence In vitro UFMylation assays with mutagenesis, Co-IP, centrosome/cilia imaging, T426 mutagenesis, and in vivo metastasis/tumor models for ArpC4, RNF20, IFT88, PARP1, METTL16, NONO

    PMID:40419786 PMID:41105513 PMID:41184530 PMID:41272290 PMID:41608626 PMID:42008680

    Open questions at the time
    • How a single ligase is targeted to such diverse substrates in different tissues is unresolved
    • Competition between substrates for limited UFL1 capacity not addressed
  10. 2025 Low

    Began implicating UFL1/UFMylation in host-pathogen defense and metabolic rewiring, extending its physiological reach beyond intrinsic cellular pathways.

    Evidence Proximity biotinylation/MS, infection assays, zebrafish models, and metabolomics for Shigella, orthoflaviviruses, and PFKAP (all preprints)

    Open questions at the time
    • Preprint-only, single-lab findings awaiting peer review
    • Direct substrates and whether UFMylation versus scaffolding drives the effects not fully resolved
    • Generalizability across pathogens unconfirmed

Open questions

Synthesis pass · forward-looking unresolved questions
  • How UFL1 substrate selectivity, kinase regulation (ATM/Akt/AMPK), and the choice between stabilizing versus destabilizing a UFMylated substrate are integrated into a unified regulatory logic remains unresolved.
  • No structural model of UFL1 bound to a substrate
  • No general code linking UFMylation site to ubiquitin antagonism or promotion
  • Coordination among UFL1's nuclear, ER, mitotic, and immune roles not mapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 6 GO:0016740 transferase activity 5 GO:0016874 ligase activity 3 GO:0060090 molecular adaptor activity 3
Localization
GO:0005783 endoplasmic reticulum 4 GO:0000228 nuclear chromosome 2 GO:0005634 nucleus 2 GO:0005815 microtubule organizing center 1
Pathway
R-HSA-392499 Metabolism of proteins 4 R-HSA-168256 Immune System 3 R-HSA-73894 DNA Repair 3 R-HSA-8953897 Cellular responses to stimuli 3 R-HSA-1640170 Cell Cycle 2 R-HSA-4839726 Chromatin organization 2
Partners
ARPC4CDK5RAP3DDRGK1IRE1AMRE11UBA5UFC1VCP
Complex memberships
UFL1–CDK5RAP3/UFBP1 complex

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2019 UFL1 is recruited to DNA double-strand breaks by the MRE11/RAD50/NBS1 (MRN) complex and monoufmylates histone H4 following DNA damage. Monoufmylated histone H4 recruits Suv39h1 and Tip60, which are required for ATM activation. ATM in turn phosphorylates UFL1 at serine 462, enhancing its E3 ligase activity in a positive feedback loop. Co-immunoprecipitation, in vitro ufmylation assay, site-directed mutagenesis (S462 phosphorylation site), chromatin immunoprecipitation, laser micro-irradiation/live imaging Nature communications High 30886146
2023 UFL1 requires its N-terminal helix for binding to UFC1 (E2). The UFL1–DDRGK1 fusion construct was used to solve a crystal structure of this critical interaction. UFL1 and UBA5 (E1) compete for binding to UFC1, with the competition shifting in favor of UFL1 after UFM1 is charged onto UFC1, coordinating the E1→E2→E3 cascade. X-ray crystallography, NMR, AlphaFold2 structural modeling, biochemical binding assays, mutagenesis EMBO reports High 37988244
2024 On replication stress, UFL1 localizes to stalled replication forks and catalyzes UFMylation of PTIP (a component of the MLL3/4 methyltransferase complex) at lysine 148. This promotes assembly of the PTIP–MLL3/4 complex, enrichment of H3K4me1 and H3K4me3 at stalled forks, and subsequent MRE11 nuclease recruitment to degrade nascent DNA in BRCA1/2-deficient cells. Proximity ligation assay, iPOND (isolation of proteins on nascent DNA), in vitro UFMylation assay, site-directed mutagenesis (K148), chromatin fractionation, fiber assay Nature chemical biology High 38649452
2024 UFL1 promotes UFMylation of PD-1 in T cells, which antagonizes PD-1 ubiquitination and prevents its degradation. AMPK phosphorylates UFL1 at Thr536, disrupting PD-1 UFMylation and triggering PD-1 degradation. Loss of UFL1 in T cells reduces PD-1 stability and enhances CD8+ T cell activation. Conditional knockout mice, Co-immunoprecipitation, in vitro UFMylation assay, site-directed mutagenesis (T536), flow cytometry, single-cell RNA sequencing Molecular cell High 38377992
2010 UFL1 (Maxer) is an ER membrane protein that interacts with CDK5RAP3, anchoring it to the ER and inhibiting CDK5RAP3's function in repressing Cyclin D1 transcription in the nucleus. Loss of Maxer leads to cell accumulation at G1 phase. Co-immunoprecipitation, subcellular fractionation, knockdown/overexpression with cell cycle analysis (FACS), immunofluorescence The EMBO journal Medium 20531390
2010 UFL1 (NLBP) was identified as a novel LZAP-binding protein. NLBP and LZAP mutually stabilize each other by inhibiting ubiquitination of the partner protein. NLBP also inhibits NF-κB signaling and cell invasion. Tandem affinity purification, Co-immunoprecipitation, ubiquitination assay, invasion assay The Journal of biological chemistry Medium 20164180
2013 UFL1 (NLBP) binds to the regulatory domain of p120 catenin (p120ctn), and this interaction stabilizes p120ctn by inhibiting its ubiquitination and proteasomal degradation, promoting lung adenocarcinoma cell proliferation. Co-immunoprecipitation, domain mapping, ubiquitination assay, overexpression proliferation assay Cell cycle Medium 23839039
2015 Genetic deletion of RCAD/UFL1 in mice causes elevated ER stress, activation of UPR, blockage of autophagic degradation, increased mitochondrial mass and ROS, DNA damage response, p53 activation, and enhanced cell death in hematopoietic stem cells, establishing UFL1 as essential for HSC survival and erythroid differentiation. Germ-line and conditional knockout mice, Western blotting for ER stress/UPR markers, autophagy flux assays, ROS measurement, flow cytometry, p53 pathway analysis Cell death and differentiation High 25952549
2019 CDK5RAP3 interacts with UFL1 in vivo, and loss of CDK5RAP3 alters the ufmylation profile in liver cells, establishing CDK5RAP3 as a substrate adaptor for UFL1-mediated UFMylation. Co-immunoprecipitation (in vivo interaction), conditional knockout mice, proteomics-based ufmylation profiling Development (Cambridge, England) Medium 30635284
2018 Cardiac-specific knockout of Ufl1 impairs PERK (PKR-like ER-resident kinase) signaling, leading to excessive ER stress, cardiomyocyte death, and development of cardiomyopathy. Administration of the ER chaperone tauroursodeoxycholic acid alleviates ER stress and attenuates cardiac dysfunction in Ufl1-deficient mice. Cardiac-specific conditional knockout mice, transcriptome analysis, biochemical ER stress markers (Western blot), chemical chaperone rescue experiment Circulation. Heart failure High 30354401
2023 The UFL1/UFBP1 (UFL1/CDK5RAP3) complex directly interacts with the mTOR/GβL complex and attenuates mTORC1 activity. Ablation of UFL1 or UFBP1 in hepatocytes dissociates them from the mTOR/GβL complex and activates oncogenic mTOR signaling, driving hepatocellular carcinoma development. Co-immunoprecipitation, hepatocyte-specific conditional knockout mice, iTRAQ proteomics, DEN/HFD liver cancer models Journal of experimental & clinical cancer research Medium 37131258
2022 UFL1 inhibits TRIM29 from interacting with STING, thereby reducing STING ubiquitination at K338/K347/K370 and preventing its proteasomal degradation. This stabilizing function of UFL1 on STING is independent of its UFMylation E3 ligase activity. Co-immunoprecipitation, ubiquitination assay with site-specific mutants (K338/K347/K370), UFL1 ligase-dead mutant, Western blotting for protein stability Cell death and differentiation Medium 35871231
2025 UFL1 promotes UFMylation of PARP1, preventing its ubiquitination and proteasomal degradation. Stabilized PARP1 enhances DNA damage repair, suppresses R-loop formation, and inhibits cGAS-STING activation, promoting tumor immune evasion in pancreatic cancer. Co-immunoprecipitation, UFMylation assay, R-loop detection, cGAS-STING reporter assay, conditional knockout tumor models, flow cytometry for CD8+ T cells Cell reports Medium 41105513
2025 Akt phosphorylates UFL1 at T426, enhancing its interaction with ArpC4 (a core subunit of the Arp2/3 complex) and inducing ArpC4 UFMylation. UFL1-mediated ArpC4 UFMylation facilitates lamellipodia formation and promotes cell migration, invasion, and metastasis. Co-immunoprecipitation, in vitro UFMylation assay, site-directed mutagenesis (T426), lamellipodia formation imaging, invasion/migration assay, in vivo metastasis models Nature structural & molecular biology High 40419786
2022 Skin-specific deletion of Ufl1 in mice causes epidermal thickening and hyperpigmentation. Mechanistically, Endothelin-1 (ET-1) is a UFMylation substrate; UFL1-mediated UFMylation of ET-1 regulates its stability. Loss of Ufl1 increases ET-1 expression and secretion, upregulating melanin biosynthesis genes. Skin-specific conditional knockout mice, RNA-Seq, in vivo UFMylation assay for ET-1, Western blotting for ET-1 stability Frontiers in cell and developmental biology Medium 36120581
2025 UFL1 interacts with RNF20 and catalyzes its UFMylation, enhancing RNF20 binding to CEP192 and facilitating its centrosomal localization to support mitotic spindle assembly. Loss of UFL1 causes mitotic defects, chromosome segregation errors, and aneuploidy in prostate cancer. Co-immunoprecipitation, UFMylation assay, centrosome fractionation/immunofluorescence, mitotic spindle analysis, conditional knockout prostate cancer models Proceedings of the National Academy of Sciences of the United States of America Medium 42008680
2025 UFL1 mediates UFMylation of IFT88 at lysine 572. This UFMylation antagonizes IFT88 ubiquitination by the E3 ligase PJA2, preventing proteasomal degradation of IFT88 and maintaining ciliary homeostasis. A K572R mutant of IFT88 (UFMylation-deficient) shows increased stability and rescues ciliary defects caused by UFL1 depletion. Conditional knockout mice, Co-immunoprecipitation, in vitro UFMylation and ubiquitination assays, site-directed mutagenesis (K572R), immunofluorescence of cilia, rescue experiments Cell death and differentiation High 41272290
2026 UFL1 UFMylates METTL16, reducing its ubiquitination and decreasing its protein stability. Loss of UFL1 increases METTL16 stability, leading to increased m6A modification of EEF1A1 mRNA via the METTL16-IGF2BP1 axis, elevating EEF1A1 protein levels and driving enzalutamide resistance in prostate cancer. Co-immunoprecipitation, UFMylation assay, ubiquitination assay, m6A methylation assay, protein stability assay, xenograft models International journal of biological sciences Medium 41608626
2025 WBP11 interacts with NONO and competitively inhibits UFL1-induced UFMylation of NONO at Lys198. Loss of WBP11 allows UFL1 to UFMylate NONO, reducing its stability. UFL1 overexpression suppresses HCC cell growth and metastasis via NONO degradation. Co-immunoprecipitation, in vitro UFMylation assay, site-directed mutagenesis (K198), competitive binding assay, xenograft models Oncogene Medium 41184530
2024 LRP1 knockdown promotes binding of UFL1 to OGA (O-GlcNAcase) and accelerates ubiquitin-mediated OGA degradation. This leads to increased O-GlcNAcylation of NF-κB and inhibition of pro-apoptotic gene expression. The LRP1 β-chain stabilizes OGA by disrupting the UFL1-OGA interaction. Co-immunoprecipitation, ubiquitination assay, O-GlcNAcylation analysis, knockdown/overexpression, xenograft models Advanced science Medium 39405202
2024 Chicken UFL1 (chUFL1) interacts with chSTING and promotes K63-linked polyubiquitination of chSTING at K308, facilitating STING dimerization and formation of the STING-TBK1-IRF7 complex for type I IFN production, independently of UFMylation. ChUFL1 also interacts with the AIV PA protein to inhibit viral polymerase activity and nuclear import of PA. Co-immunoprecipitation, ubiquitination assay with K63-specific analysis, site-directed mutagenesis (K308), STING dimerization assay, UFMylation-dead mutant, viral polymerase activity assay Journal of immunology Medium 38477617
2024 UFL1 interacts with IRE1α and modulates the IRE1α/XBP1 pathway of the unfolded protein response in NEFA-stimulated bovine mammary epithelial cells, contributing to ER and mitochondrial homeostasis. Co-immunoprecipitation, Western blotting for IRE1α/XBP1 pathway markers, UFL1 knockdown/overexpression Free radical biology & medicine Low 38821134
2026 UFL1 deficiency in skeletal muscle activates the PERK/eIF2α/ATF4/CHOP ER stress signaling axis, leading to myoblast apoptosis. Pharmacological inhibition of PERK (GSK2606414) reverses UFL1-deficiency-induced upregulation of p-PERK, p-eIF2α, ATF4, and CHOP and rescues the apoptotic phenotype. Skeletal muscle-specific knockout mice, C2C12 cells, Western blotting, PERK inhibitor rescue, apoptosis assays Cellular signalling Medium 42019645
2025 ATI-1 targets VCP/p97 and disrupts its interaction with UFL1. This disruption promotes polyubiquitination and proteasomal degradation of Beclin1, inhibiting autophagy initiation. The VCP-UFL1-Beclin1 axis is identified as a regulatory node in autophagy. Co-immunoprecipitation (VCP-UFL1 interaction), ubiquitination assay for Beclin1, autophagy flux assays, small-molecule inhibitor (ATI-1) treatment, xenograft models Bioorganic chemistry Low 42060985
2025 UFL1 interacts with multiple orthoflavivirus proteins (NS2A, NS2B-NS3, and Capsid) and promotes infectious virion production for dengue, Zika, West Nile, and yellow fever viruses. UFMylation does not regulate viral RNA translation or replication but acts at a later stage, likely viral assembly. Co-immunoprecipitation (UFL1 with viral proteins), infectious particle assays, siRNA depletion of UFL1/UFBP1/UBA5/UFC1/UFM1, RNA replication assays bioRxivpreprint Low
2025 Intracellular Shigella bacteria become targeted by UFL1 in infected human cells. Shigella antagonizes UFL1-mediated UFMylation in two ways: LPS shields bacteria from UFL1 recruitment, and the bacterial effector IpaH9.8 prevents UFM1 decoration. Loss of UFMylation increases bacterial burden in human cells and zebrafish, and this protective role is independent of autophagy. Proximity biotinylation coupled to quantitative mass spectrometry, bacterial infection assays, LPS mutant bacteria, IpaH9.8 effector assays, zebrafish infection model, autophagy-independent validation bioRxivpreprint Low
2025 Loss of UFMylation (via UFL1 loss) reduces phosphofructokinase (PFKAP) activity, rerouting glucose metabolism away from glycolysis toward the hexosamine biosynthesis pathway. Elevated hexosamine biosynthesis increases glycosylation of invasion-related proteins, promoting prostate cancer metastasis. PFKAP is identified as a UFMylation substrate. Biotin-based proximity proteomics (UFMylation substrate ID), metabolomics, UFL1 knockdown/overexpression, invasion assays, in vivo metastasis models bioRxivpreprint Low

Source papers

Stage 0 corpus · 51 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2015 RCAD/Ufl1, a Ufm1 E3 ligase, is essential for hematopoietic stem cell function and murine hematopoiesis. Cell death and differentiation 160 25952549
2019 UFL1 promotes histone H4 ufmylation and ATM activation. Nature communications 154 30886146
2018 Ufm1-Specific Ligase Ufl1 Regulates Endoplasmic Reticulum Homeostasis and Protects Against Heart Failure. Circulation. Heart failure 81 30354401
2019 CDK5RAP3, a UFL1 substrate adaptor, is crucial for liver development. Development (Cambridge, England) 75 30635284
2010 Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity. The EMBO journal 67 20531390
2024 UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity. Molecular cell 62 38377992
2010 A novel LZAP-binding protein, NLBP, inhibits cell invasion. The Journal of biological chemistry 56 20164180
2019 UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells. Oxidative medicine and cellular longevity 50 30881595
2020 UFL1 Alleviates LPS-Induced Apoptosis by Regulating the NF-κB Signaling Pathway in Bovine Ovarian Granulosa Cells. Biomolecules 44 32050508
2022 UFL1 promotes antiviral immune response by maintaining STING stability independent of UFMylation. Cell death and differentiation 43 35871231
2019 UFL1 modulates NLRP3 inflammasome activation and protects against pyroptosis in LPS-stimulated bovine mammary epithelial cells. Molecular immunology 39 31078114
2020 UFL1 attenuates IL-1β-induced inflammatory response in human osteoarthritis chondrocytes. International immunopharmacology 38 32050156
2019 Ufl1/RCAD, a Ufm1 E3 ligase, has an intricate connection with ER stress. International journal of biological macromolecules 33 31129212
2023 Loss of Ufl1/Ufbp1 in hepatocytes promotes liver pathological damage and carcinogenesis through activating mTOR signaling. Journal of experimental & clinical cancer research : CR 30 37131258
2013 Overexpression of a novel regulator of p120 catenin, NLBP, promotes lung adenocarcinoma proliferation. Cell cycle (Georgetown, Tex.) 29 23839039
2024 UFL1 triggers replication fork degradation by MRE11 in BRCA1/2-deficient cells. Nature chemical biology 27 38649452
2023 UFL1, a UFMylation E3 ligase, plays a crucial role in multiple cellular stress responses. Frontiers in endocrinology 27 36843575
2017 RCAD/BiP pathway is necessary for the proper synthesis of digestive enzymes and secretory function of the exocrine pancreas. American journal of physiology. Gastrointestinal and liver physiology 25 28104585
2021 Ufl1 deficiency causes kidney atrophy associated with disruption of endoplasmic reticulum homeostasis. Journal of genetics and genomics = Yi chuan xue bao 24 34148841
2024 Panaxatriol exerts anti-senescence effects and alleviates osteoarthritis and cartilage repair fibrosis by targeting UFL1. Journal of advanced research 19 39442872
2023 Structural study of UFL1-UFC1 interaction uncovers the role of UFL1 N-terminal helix in ufmylation. EMBO reports 19 37988244
2002 Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG. Molecular microbiology 17 11929529
2022 UFL1 alleviates ER stress and apoptosis stimulated by LPS via blocking the ferroptosis pathway in human granulosa-like cells. Cell stress & chaperones 16 35729487
1998 Promoter recognition by a cyanobacterial RNA polymerase: in vitro studies with the Calothrix sp. PCC 7601 transcriptional factors RcaA and RcaD. Plant molecular biology 16 9526497
1995 Thirteen genes (Cebpb, E2f1, Tcf4, Cyp24, Pck1, Acra4, Edn3, Kcnb1, Mc3r, Ntsr, Cd40, Plcg1 and Rcad) that probably lie in the distal imprinting region of mouse chromosome 2 are not monoallelically expressed. Genetical research 13 7781998
2024 RCAd-LTH-shPD-L1, a double-gene recombinant oncolytic adenovirus with enhanced antitumor immunity, increases lymphocyte infiltration and reshapes the tumor microenvironment. Journal for immunotherapy of cancer 10 38212125
2019 Protective effects of UFL1 against endoplasmic reticulum stress-induced autophagy in bovine mammary epithelial cells. Cell stress & chaperones 10 31721015
2014 Dissecting the roles of E1A and E1B in adenoviral replication and RCAd-enhanced RDAd transduction efficacy on tumor cells. Cancer biology & therapy 9 25019940
2024 Loss of LRP1 Promotes Hepatocellular Carcinoma Progression via UFL1-Mediated Activation of NF-κB Signaling. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8 39405202
2025 Akt-phosphorylated UFL1 UFMylates ArpC4 to promote metastasis. Nature structural & molecular biology 7 40419786
2012 A novel immunocompetent murine tumor model for the evaluation of RCAd-enhanced RDAd transduction efficacy. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 7 22627833
2024 Bombyx mori UFL1 facilitates BmNPV proliferation by regulating host cell apoptosis through PERK. Archives of insect biochemistry and physiology 5 38976652
2022 Cadherin switches during epithelial-mesenchymal transition: CDH4/RCAD downregulation reduces bladder cancer progression. Cellular oncology (Dordrecht, Netherlands) 5 35064910
2018 Metabolic Profiling of Multiorgan Samples: Evaluation of MODY5/RCAD Mutant Mice. Journal of proteome research 5 29873499
2025 Targeting the UFL1-PARP1 axis amplifies anti-tumor immunity. Cell reports 4 41105513
2024 Chicken UFL1 Restricts Avian Influenza Virus Replication by Disrupting the Viral Polymerase Complex and Facilitating Type I IFN Production. Journal of immunology (Baltimore, Md. : 1950) 4 38477617
2024 UFL1 regulates cellular homeostasis by targeting endoplasmic reticulum and mitochondria in NEFA-stimulated bovine mammary epithelial cells via the IRE1α/XBP1 pathway. Free radical biology & medicine 4 38821134
2011 rCAD: A Novel Database Schema for the Comparative Analysis of RNA. Proceedings ... IEEE International Conference on eScience. IEEE International Conference on eScience 4 24772454
2025 WBP11 inhibits UFL1-mediated UFMylation of NONO to drive hepatocellular carcinoma progression. Oncogene 3 41184530
2022 Ufl1 deficiency causes skin pigmentation by up-regulation of Endothelin-1. Frontiers in cell and developmental biology 3 36120581
2020 Two potential molecular signaling pathways of the UFL1 gene to induce the endoplasmic reticulum stress and apoptosis of the ovarian granulosa cell. Medical hypotheses 3 33035966
2010 The renal cysts and diabetes (RCAD) syndrome in a child with deletion of the hepatocyte nuclear factor-1β gene. Indian journal of pediatrics 3 20890685
2025 UFL1 promotes survival and function of virtual memory CD8 T cells. Journal of immunology (Baltimore, Md. : 1950) 2 40073095
2025 UFL1-mediated UFMylation antagonizes IFT88 ubiquitination and degradation to maintain ciliary homeostasis. Cell death and differentiation 2 41272290
2022 Elevated level of lysophosphatidic acid among patients with HNF1B mutations and its role in RCAD syndrome: a multiomic study. Metabolomics : Official journal of the Metabolomic Society 2 35179657
2026 Loss of UFL1 confers enzalutamide resistance of prostate tumors by regulating METTL16-mediated m6A modification of EEF1A1 mRNA. International journal of biological sciences 1 41608626
2026 UFL1 deficiency disrupts skeletal muscle lipid metabolism by promoting the ACC1-FASN axis. Biochemical and biophysical research communications 0 41967450
2026 Prenatal Diagnosis of Renal Cysts and Diabetes Syndrome (RCAD): A Case Report. The American journal of case reports 0 41999034
2026 Loss of UFL1 drives chromosome instability and tumorigenesis of prostate cancer. Proceedings of the National Academy of Sciences of the United States of America 0 42008680
2026 UFL1 deficiency impairs skeletal muscle development by activating PERK/eIF2α/ATF4/CHOP pathway-dependent apoptosis. Cellular signalling 0 42019645
2026 ATI-1 mediated disruption of the VCP-UFL1-Beclin1 axis thwarts autophagy initiation to trigger metabolic catastrophe in autophagy-addicted cancers. Bioorganic chemistry 0 42060985

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