Affinage

VCL

Vinculin · UniProt P18206

Round 2 corrected
Length
1134 aa
Mass
123.8 kDa
Annotated
2026-04-28
64 papers in source corpus 20 papers cited in narrative 20 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Vinculin is an autoinhibited mechanosensory adaptor protein that couples integrin- and cadherin-based adhesion complexes to the actin cytoskeleton, functioning as a central hub for force transmission and mechanotransduction at focal adhesions and adherens junctions. In its resting state, an intramolecular head–tail interaction masks the C-terminal F-actin binding site; simultaneous engagement of talin via the head domain and F-actin via the tail domain relieves autoinhibition, enabling vinculin to promote integrin clustering, recruit the Arp2/3 complex through its hinge region for localized actin polymerization, and protect VE-cadherin junctions from force-induced opening (PMID:7816144, PMID:12473693, PMID:22391038, PMID:18056416). In cardiomyocytes, vinculin recruits the SSH1–cofilin axis to regulate F-actin rearrangement required for sarcomere maturation, and phosphorylation at tyrosine 822 governs the balance between cadherin- and integrin-based adhesion organization (PMID:31495694). Both loss-of-function and gain-of-function VCL variants have been identified in human neural tube defects, indicating that precise vinculin dosage is critical for morphogenetic cell movements (PMID:33491343).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1995 High

    Establishing the autoinhibition mechanism resolved how vinculin exists in an inactive cytoplasmic pool despite possessing potent F-actin binding activity: the 95 kDa head domain masks the tail's actin-binding site, and activation requires disruption of this intramolecular contact.

    Evidence Cosedimentation, crosslinking, and electron microscopy of bacterially expressed head and tail domain fragments

    PMID:7816144

    Open questions at the time
    • The physiological signals that relieve autoinhibition in cells were not identified
    • Structural details of the head–tail interface at atomic resolution were not resolved
    • Whether intermediate partially open states exist was not addressed
  2. 1999 High

    Identification of paxillin LD motifs as direct vinculin-binding interfaces established vinculin as a scaffold component linking signaling adaptors to the focal adhesion cytoskeleton.

    Evidence GST pulldown of LD motif–vinculin interaction; dominant-negative LD peptide microinjection disrupts migration

    PMID:10330411

    Open questions at the time
    • Whether paxillin–vinculin binding requires vinculin activation or occurs with the autoinhibited form was unclear
    • Relative contribution of paxillin versus talin to vinculin recruitment was not quantified
  3. 2002 High

    Discovery that vinculin's hinge region directly recruits the Arp2/3 complex in a PIP2- and Rac1-dependent manner revealed a mechanism by which integrin engagement is coupled to actin polymerization and lamellipodial protrusion.

    Evidence Co-IP, GST pulldown, point mutagenesis of the hinge region, vinculin-null cell rescue and spreading assays

    PMID:12473693

    Open questions at the time
    • Whether Arp2/3 binding is compatible with the autoinhibited conformation was not resolved
    • The temporal sequence of Arp2/3 recruitment relative to talin binding was not established
  4. 2007 High

    Domain-dissection in vinculin-null cells demonstrated that the head domain promotes integrin clustering and residency via talin, while the tail domain links adhesions to the actin force-transmission network, providing a bipartite model for vinculin's role in focal adhesion growth.

    Evidence Head/tail mutant expression, FRAP, integrin clustering assays, TIRF microscopy in vinculin-null MEFs

    PMID:18056416

    Open questions at the time
    • Force dependence of head-mediated integrin clustering was not measured
    • Whether tail-mediated actin coupling requires catch-bond behavior was unknown
  5. 2012 High

    Extending vinculin's role beyond focal adhesions, demonstration that vinculin protects VE-cadherin junctions from force-induced opening established it as a mechanosensory effector at endothelial adherens junctions.

    Evidence Vinculin-null endothelial cells, α-catenin vinculin-binding-deficient mutant rescue, Rho-Rock inhibition, live-cell tension measurement

    PMID:22391038

    Open questions at the time
    • How vinculin is activated specifically at cadherin versus integrin adhesions was not resolved
    • Whether vinculin at adherens junctions recruits Arp2/3 as at focal adhesions was untested
  6. 2015 High

    Placing vinculin downstream of ZO-1–JACOP–p114RhoGEF–Rho signaling at endothelial junctions defined the upstream pathway controlling vinculin recruitment to mechanically loaded cadherin complexes.

    Evidence siRNA epistasis, FRET tension sensors on VE-cadherin, co-IP, endothelial barrier assays

    PMID:25753039

    Open questions at the time
    • Whether this signaling axis operates at epithelial or other cadherin junctions was not tested
    • Direct phosphorylation or conformational activation by this pathway was not shown
  7. 2019 High

    In cardiomyocytes, vinculin was shown to be essential for myofilament maturation by recruiting the SSH1–cofilin axis to regulate F-actin dynamics in response to contractile forces, extending vinculin's function to sarcomere assembly.

    Evidence Zebrafish vcl knockout, quantitative interactomics (MS), co-IP of VCL–SSH1–CFL, live imaging of contracting hearts

    PMID:31495694

    Open questions at the time
    • Whether SSH1 binding requires vinculin activation or a specific phosphorylation state was not determined
    • The structural basis for the VCL–SSH1 interaction was not mapped
  8. 2021 Medium

    Identification of both loss-of-function and gain-of-function VCL variants in human neural tube defects demonstrated that precise vinculin dosage is critical for PCP-dependent morphogenetic cell movements during development.

    Evidence Targeted NGS in NTD cohort, PCP pathway reporter assay, migration assays, protein stability analysis

    PMID:33491343

    Open questions at the time
    • Variants identified in a single cohort without replication in independent populations
    • How VCL gain-of-function enhances PCP signaling mechanistically was not resolved
    • Animal model validation of neural tube phenotype for these specific variants was not performed
  9. 2021 Medium

    Discovery that tankyrase PARylates vinculin at conserved TBM motifs at epithelial adherens junctions introduced PARylation as a post-translational regulatory mechanism controlling vinculin function and epithelial cell shape.

    Evidence PAR affinity precipitation, TBM-II point mutant overexpression induces mesenchymal shape change, TNKS inhibitor treatment

    PMID:34123588

    Open questions at the time
    • Specific PARylated residues were not mapped
    • How PARylation affects vinculin autoinhibition or binding partners was not determined
    • No loss-of-function genetic approach for endogenous VCL PARylation
  10. 2023 Medium

    Validation of miR-29a-3p as a direct negative regulator of VCL expression linked miRNA-mediated vinculin downregulation to epithelial barrier disruption in allergic rhinitis, extending vinculin regulation to post-transcriptional control in disease contexts.

    Evidence Dual-luciferase 3ʹ-UTR reporter, miR-29a-3p mimic/antagomir, OVA-induced AR mouse model with barrier function rescue

    PMID:37262956

    Open questions at the time
    • Whether miR-29a-3p regulation of VCL operates in other barrier tissues was not tested
    • Relative contribution of VCL versus CTNNB1 downregulation to barrier loss was not separated
  11. 2025 Medium

    Evidence that extracellular/plasma VCL levels correlate with vascular leakage and that anti-VCL intervention restores alveolar-capillary barrier integrity in lung injury expanded vinculin's relevance from intracellular mechanotransduction to a potential circulating mediator of vascular pathology.

    Evidence Multi-omics proteomics of COVID-19 patient plasma, anti-VCL antibody intervention in rat lung injury model, histology and electron microscopy

    PMID:40268929

    Open questions at the time
    • Mechanism by which extracellular VCL promotes vascular leakage is unknown
    • Whether plasma VCL is actively secreted or a passive release marker was not distinguished
    • Single preclinical model without independent replication

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis and in vivo dynamics of vinculin's catch-bond with F-actin, whether distinct post-translational modifications (pY822, PARylation) regulate autoinhibition relief or partner selectivity, and the functional significance of alternative VCL splice isoforms generated by RBM10-dependent splicing in cancer cell migration.
  • Atomic-resolution structure of vinculin's catch bond with actin under force is lacking
  • Interplay between pY822 phosphorylation and PARylation in regulating cadherin vs. integrin adhesion balance is unexplored
  • In vivo functional validation of the RBM10-regulated pro-migratory VCL splice isoform beyond preprint data

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 5 GO:0060090 molecular adaptor activity 4 GO:0005198 structural molecule activity 3
Localization
GO:0005886 plasma membrane 5 GO:0005856 cytoskeleton 4
Pathway
R-HSA-1500931 Cell-Cell communication 4 R-HSA-162582 Signal Transduction 3 R-HSA-1266738 Developmental Biology 2 R-HSA-1474244 Extracellular matrix organization 2
Partners
ACTR2CDH5CFL1CTNNA1PXNSSH1TLN1TNKS
Complex memberships
Cadherin–catenin adherens junction complexFocal adhesion complex

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 Vinculin (VCL) contains an intramolecular head-tail autoinhibitory interaction: the 95 kDa head domain masks an F-actin binding site located in the 30 kDa carboxy-terminal tail domain (residues 811–1066). Intact vinculin does not cosediment with F-actin, but isolated tail fragments do, and the head fragment inhibits this interaction, demonstrating that activation requires disruption of the head-tail association. Cosedimentation assays, crosslinking, transmission electron microscopy, bacterially expressed domain fragments, proteolytic fragmentation Nature High 7816144
1999 Paxillin LD motifs function as selective protein-binding interfaces; LD motifs mediate direct binding to vinculin as part of focal adhesion scaffold assembly, implicating vinculin in paxillin-organized cytoskeletal remodeling at focal adhesions. GST pulldown, microinjection of LD motif peptides, GFP localization, cell migration wound assay The Journal of Cell Biology High 10330411
2002 The Arp2/3 complex directly binds to the hinge region of vinculin in a phosphatidylinositol-4,5-bisphosphate- and Rac1-dependent manner. This interaction recruits Arp2/3 to new integrin adhesion sites and promotes lamellipodial protrusion and cell spreading; a point mutation in the hinge region selectively blocks Arp2/3 binding and reduces spreading on fibronectin. Co-immunoprecipitation, GST pulldown, domain mapping with point mutagenesis, vinculin-null cell rescue, spreading/protrusion assays The Journal of Cell Biology High 12473693
1992 The vinculin gene (VCL) maps to chromosomal band 10q22.1–q23, distal to D10S22, established by somatic hybrid panel hybridization, genetic recombination mapping in MEN2 families, and flow-sorted translocation chromosome hybridization. Somatic cell hybrid panel, genetic linkage mapping, flow-sorted chromosome hybridization Genomics Medium 1505973
2007 Vinculin head domain regulates integrin dynamics and clustering while the tail domain links focal adhesions to the actin force-transmission machinery. Vinculin constructs with unmasked head and tail binding sites induce dramatic focal adhesion growth through direct interaction with talin, which promotes integrin clustering and increases integrin residency time. Paxillin recruitment at focal adhesions occurs independently of the vinculin tail's paxillin-binding site. Vinculin head/tail mutant expression, FRAP, integrin clustering assays, co-immunoprecipitation, TIRF microscopy in vinculin-null cells The Journal of Cell Biology High 18056416
2012 Vinculin associates with VE-cadherin-based adherens junctions (focal adherens junctions, FAJs) that are attached to radial F-actin bundles and subjected to actomyosin-generated pulling forces. Vinculin protects VE-cadherin junctions from opening during force-dependent remodeling induced by VEGF, TNF-α, or thrombin. FAJ formation requires Rho-Rock-actomyosin contractility but not vinculin itself; however, vinculin loss results in junction opening under force. Live-cell imaging, vinculin-null endothelial cells, Rho-Rock inhibition, α-catenin vinculin-binding-deficient mutant, tension measurement The Journal of Cell Biology High 22391038
2015 ZO-1 depletion in endothelial cells reduces tension on VE-cadherin and causes loss of junctional mechanotransducers including vinculin, inducing vinculin dissociation from the α-catenin–VE-cadherin complex. This places vinculin downstream of ZO-1–JACOP–p114RhoGEF–Rho signaling in regulation of actomyosin-dependent junction tension. siRNA knockdown, FRET tension sensors, immunofluorescence, co-immunoprecipitation, endothelial barrier assays The Journal of Cell Biology High 25753039
2017 Mechanistic review consolidating that vinculin is recruited to and activated at both integrin-based focal adhesions and cadherin-based adherens junctions; its autoinhibited head-tail conformation is relieved by simultaneous binding of talin (head) and actin (tail), enabling force transmission and cytoskeletal linkage at adhesion complexes. Review integrating structural, biochemical, and cell biological evidence Cellular and Molecular Life Sciences Medium 28401269
2019 In contracting cardiomyocytes, mechanical forces from the heartbeat regulate vinculin (VCL) localization and activation. VCL is essential for myofilament maturation in the developing zebrafish heart. Interactome analysis in contracting vs. non-contracting cardiomyocytes identified slingshot protein phosphatase SSH1 as a VCL interactor; VCL recruits SSH1 and its effector cofilin (CFL) to regulate F-actin rearrangement and promote sarcomere myofilament maturation. Zebrafish genetic model (vcl knockout), quantitative interactomics (mass spectrometry), co-immunoprecipitation, F-actin staining, live imaging of contracting vs. non-contracting hearts Developmental Cell High 31495694
2021 Loss-of-function (p.D256fs) and gain-of-function (p.L555V) VCL variants cause human neural tube defects. p.L555V increases vinculin protein stability and enhances PCP pathway regulation and cell migration, demonstrating that both reduced and excess VCL function disrupt neural tube closure. Targeted NGS in NTD cohort, in vitro functional assays (PCP pathway reporter, migration assays), protein stability analysis Molecular Genetics & Genomic Medicine Medium 33491343
2021 Vinculin contains conserved tankyrase-binding motifs (TBMs) in vertebrates (absent in C. elegans). Tankyrase (TNKS) localizes to the plasma membrane belt in epithelial cells, a VCL pool is covalently PARylated (poly-ADP-ribosylated), and overexpression of a VCL TBM-II point mutant induces mesenchymal-like cell shape changes, suggesting TNKS-mediated PARylation of VCL regulates epithelial adherens junction integrity and cell shape. Sequence conservation analysis, TNKS inhibitor treatment, immunocytofluorescence, subcellular fractionation, PAR affinity precipitation + western blot, transfection of TBM mutant VCL PeerJ Medium 34123588
2024 miR-6721-5p directly interacts with the 3'-UTR of meta-VCL (the muscle-specific large splice isoform of VCL) and negatively regulates its expression. Upregulation of miR-6721-5p in CAD patients correlates with reduced meta-VCL and decreased anti-inflammatory cytokines IL-10 and TNF-α. Dual-luciferase 3'-UTR reporter assay, qPCR, ELISA for cytokines, bioinformatics, ROC curve analysis of serum samples Non-coding RNA Research Medium 39296643
2023 miR-29a-3p directly targets the 3'-UTR of VCL (and CTNNB1) in nasal epithelial cells, reducing VCL protein expression. VCL and β-catenin contribute to adherens junction and tight junction integrity of nasal mucosa; miR-29a-3p upregulation in allergic rhinitis disrupts epithelial barrier function, which is partially rescued by miR-29a-3p antagomir in OVA-induced AR mice. Dual-luciferase reporter assay, miRNA mimic/inhibitor transfection, OVA-induced AR mouse model, qPCR, antagomir treatment, barrier function assays International Immunopharmacology Medium 37262956
2025 VCL plasma levels are elevated in Omicron SARS-CoV-2 infection and correlate with inflammatory markers and lung exudation. Anti-VCL intervention in a rat lung injury model reduces plasma VCL levels, mitigates alveolar edema, and restores alveolar-capillary barrier integrity, demonstrating that VCL modulation affects vascular leakage and extravasation. Multi-omics proteomics/metabolomics of human plasma, rat lung injury model, anti-VCL antibody intervention, histological staining, electron microscopy Nature Communications Medium 40268929
2024 VCL is identified as a novel CRBN (cereblon) neosubstrate degradable by molecular glue degraders; ubiquitinomics analysis confirms VCL ubiquitylation upon treatment with phenyl glutarimide-based degraders, enabling targeted proteasomal degradation of VCL without a classical CRBN degron motif. High-throughput proteomics + ubiquitinomics (DIA-MS), 100-compound CRBN-ligand screen across cancer cell lines bioRxiv (preprint)preprint Medium bio_10.1101_2024.10.18.618633
2024 RBM10 loss causes exon inclusion in VCL pre-mRNA; knockdown of the VCL exon-inclusion isoform in RBM10-null cells reduces cell velocity, while combined knockdown of VCL, CD44, and TNC exon-inclusion isoforms reverses metastasis in HrasG12V/Rbm10-KO mouse thyrocytes, demonstrating a specific pro-migratory function of the VCL exon-inclusion isoform. RNA-seq, isoform-specific siRNA knockdown, cell velocity assay, mouse Hras/Rbm10 KO tumor model, CRISPR screen bioRxiv (preprint)preprint Medium bio_10.1101_2024.07.09.602730
2025 In Ccm1-deficient zebrafish, endothelial vinculin displays excessive mechanically active focal adhesions in vivo. Genetic deletion of Talin1 to decouple focal adhesions demonstrates that the integrin β1–Talin1 complex acts independently of or downstream of KLF2/4 to drive endothelial cell enlargement and vascular malformation in CCM1-deficiency, repositioning vinculin-associated focal adhesion signaling in CCM pathogenesis. Live imaging of vinculin in ccm1 zebrafish mutants, Talin1 genetic deletion, CCM1-KO endothelial cells, force redistribution measurements bioRxiv (preprint)preprint Medium bio_10.1101_2025.11.25.688491
2024 Vinculin phosphorylation at tyrosine 822 (pY822) correlates with dynamic junction remodeling in the developing mouse heart and is lost as junctions mature postnatally. Homozygous Y822F knock-in mice develop cardiac dysfunction by 28 weeks; Y822F hearts show reduced vinculin and adherens junction proteins at cardiomyocyte junctions and increased α5/β1 integrin and fibronectin along lateral borders, demonstrating that pY822 regulates the balance between cadherin-based and integrin-based adhesion organization in cardiomyocytes. Y822F knock-in mouse model, cardiac function assessment (echocardiography), immunofluorescence quantification of junction proteins, biochemical fractionation bioRxiv (preprint)preprint Medium bio_10.1101_2024.10.28.620745
2024 Molecular dynamics simulations with enhanced sampling reveal that vinculin forms a catch bond with F-actin: force application increases bond lifetime, and this behavior is direction-dependent. Force shifts vinculin between weakly- and strongly-bound states, with both states having intrinsic catch bonding character; directional force promotes one state over the other, providing mechanistic insight into vinculin's role in mechanotransduction at focal adhesions. All-atom molecular dynamics simulation with enhanced sampling; comparison of predicted unbinding times with single-molecule experimental data bioRxiv (preprint)preprint Medium bio_10.1101_2024.10.10.617580
2025 Integrating FRET-based vinculin tension sensors (VinTS) with traction force microscopy reveals that at the cell level, vinculin tension and cellular tractions both increase with substrate stiffness. At the focal adhesion level, vinculin tension correlates with vinculin density while tractions scale with FA area and total vinculin content. Sub-FA analysis shows tension and traction both increase toward the cell periphery, establishing a multiscale mechanotransduction framework for vinculin. FRET vinculin tension sensor (VinTS), traction force microscopy (TFM), sub-FA spatial analysis bioRxiv (preprint)preprint Medium bio_10.1101_2025.01.09.632081

Source papers

Stage 0 corpus · 64 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 2861 17081983
2005 Towards a proteome-scale map of the human protein-protein interaction network. Nature 2090 16189514
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2019 Blood-Brain Barrier: From Physiology to Disease and Back. Physiological reviews 1645 30280653
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2007 Vinculin controls focal adhesion formation by direct interactions with talin and actin. The Journal of cell biology 678 18056416
1995 Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence. Nature 660 7566098
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2003 Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions. Blood 616 14630798
2008 Large-scale proteomics and phosphoproteomics of urinary exosomes. Journal of the American Society of Nephrology : JASN 607 19056867
2011 Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation. Nature cell biology 490 21423176
2003 Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nature biotechnology 485 12665801
2015 ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation. The Journal of cell biology 452 25753039
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2015 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell 433 26638075
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2010 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.) 421 20360068
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
1999 Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling. The Journal of cell biology 402 10330411
2012 Vinculin associates with endothelial VE-cadherin junctions to control force-dependent remodeling. The Journal of cell biology 390 22391038
2017 Vinculin in cell-cell and cell-matrix adhesions. Cellular and molecular life sciences : CMLS 350 28401269
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2002 Recruitment of the Arp2/3 complex to vinculin: coupling membrane protrusion to matrix adhesion. The Journal of cell biology 339 12473693
1995 F-actin binding site masked by the intramolecular association of vinculin head and tail domains. Nature 329 7816144
2010 Renal cell carcinoma with novel VCL-ALK fusion: new representative of ALK-associated tumor spectrum. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 174 21076462
2014 VCL-ALK renal cell carcinoma in children with sickle-cell trait: the eighth sickle-cell nephropathy? The American journal of surgical pathology 79 24698962
1994 Abolition of cyclic flow variations in stenosed, endothelium-injured coronary arteries in nonhuman primates with a peptide fragment (VCL) derived from human plasma von Willebrand factor-glycoprotein Ib binding domain. Circulation 62 7994845
2019 Mechanical Forces Regulate Cardiomyocyte Myofilament Maturation via the VCL-SSH1-CFL Axis. Developmental cell 43 31495694
1995 VCL, an antagonist of the platelet GP1b receptor, markedly inhibits platelet adhesion and intimal thickening after balloon injury in the rat. Circulation 39 7648675
1995 Antithrombotic effect of a recombinant von Willebrand factor, VCL, on nitrogen laser-induced thrombus formation in guinea pig mesenteric arteries. Thrombosis and haemostasis 26 7792749
2013 Polymorphisms in FGF12, VCL, CX43 and VAX1 in Brazilian patients with nonsyndromic cleft lip with or without cleft palate. BMC medical genetics 24 23679094
1996 Adhesion of blood platelets is inhibited by VCL, a recombinant fragment (leucine504 to lysine728) of von Willebrand factor. Arteriosclerosis, thrombosis, and vascular biology 21 8548428
2021 ALK-rearranged Renal Cell Carcinoma (RCC): A Report of 2 Cases and Review of the Literature Emphasizing the Distinction Between VCL-ALK and Non-VCL-ALK RCC. International journal of surgical pathology 20 33729862
2021 Epithelioid inflammatory myofibroblastic sarcoma with VCL-ALK fusion of central nervous system: case report and brief review of the literature. Brain tumor pathology 18 34743247
2010 Identification of protein interaction regions of VINC/NEAT1/Men epsilon RNA. FEBS letters 18 20211624
2009 VCL-CB01, an injectable bivalent plasmid DNA vaccine for potential protection against CMV disease and infection. Current opinion in molecular therapeutics 17 19806506
2020 Promotive effect of Talin-1 protein on gastric cancer progression through PTK2-PXN-VCL-E-Cadherin-CAPN2-MAPK1 signaling axis. Journal of clinical laboratory analysis 16 32951272
2019 Targeted next-generation sequencing revealed distinct clinicopathologic and molecular features of VCL-ALK RCC: A unique case from an older patient without clinical evidence of sickle cell trait. Pathology, research and practice 13 31563285
2011 Familial dilated cardiomyopathy associated with congenital defects in the setting of a novel VCL mutation (Lys815Arg) in conjunction with a known MYPBC3 variant. Cardiogenetics 13 24062880
2012 Anthraquinone antitumour agents, doxorubicin, pirarubicin and benzoperimidine BP1, trigger caspase-3/caspase-8-dependent apoptosis of leukaemia sensitive HL60 and resistant HL60/VINC and HL60/DOX cells. Anti-cancer drugs 11 22198116
1992 Complementary physical and genetic techniques map the vinculin (VCL) gene on chromosome 10q. Genomics 11 1505973
2008 Thermochemistry of the gaseous vanadium chlorides VCl, VCl2, VCl3, and VCl4. The journal of physical chemistry. A 10 18774788
2008 TRAIL recombinant adenovirus triggers robust apoptosis in multidrug-resistant HL-60/Vinc cells preferentially through death receptor DR5. Human gene therapy 8 18476767
2024 MiR-6721-5p as a natural regulator of Meta-VCL is upregulated in the serum of patients with coronary artery disease. Non-coding RNA research 6 39296643
2013 Retaining cytotoxic activity of anthrapyridone CO1 against multidrug resistant cells is related to the ability to induce concomitantly apoptosis and lysosomal death of leukaemia HL60/VINC and HL60/DOX cells. The Journal of pharmacy and pharmacology 6 23647679
2008 Cationic poly(VCL-AETA) hydrogels and ovalbumin (OVA) release in vitro. Journal of materials science. Materials in medicine 6 18642060
2023 Cutaneous VCL::ALK fusion ovoid-spindle cell neoplasm. Journal of cutaneous pathology 5 36843055
2024 Osteoblastic Cell Sheet Engineering Using P(VCL-HEMA)-Based Thermosensitive Hydrogels Doped with pVCL@Icariin Nanoparticles Obtained with Supercritical CO2-SAS. Pharmaceutics 4 39204408
2015 Sex-specific association of rs4746172 of VCL gene with hypertension in two Han populations from Southern China. Scientific reports 4 26487440
2008 Crystallization and preliminary X-ray analysis of vicenisaminyltransferase VinC. Acta crystallographica. Section F, Structural biology and crystallization communications 4 18540075
2023 MiR-29a-3p promotes nasal epithelial barrier dysfunction via direct targeting of CTNNB1-VCL module in allergic rhinitis. International immunopharmacology 3 37262956
2022 MicroRNA-21-5p Regulates CD3+T Lymphocytes Through VCL and LTF in Patients with Immune Thrombocytopenia. Clinical laboratory 3 35975534
2024 Identification of a hub gene VCL for atherosclerotic plaques and discovery of potential therapeutic targets by molecular docking. BMC medical genomics 2 38287421
2021 Loss-of-function or gain-of-function variations in VINCULIN (VCL) are risk factors of human neural tube defects. Molecular genetics & genomic medicine 2 33491343
2021 First body of evidence suggesting a role of a tankyrase-binding motif (TBM) of vinculin (VCL) in epithelial cells. PeerJ 2 34123588
2025 VCL/ICAM-1 pathway is associated with lung inflammatory damage in SARS-CoV-2 Omicron infection. Nature communications 1 40268929
2024 VCL::ROS1: A Novel ROS1 Oncogenic Fusion Detected on Next Generation Sequencing. Clinical pathology (Thousand Oaks, Ventura County, Calif.) 1 39139859
2023 [No Effect of the p.Arg230His Variant Of The VCL Protein on the Course of the Hypertrophic Cardiomyopathy In Russian Family Carrying The p.Gln1233Ter Pathogenic Variant In The MYBPC3 Gene]. Kardiologiia 1 37061858