Affinage

TLN1

Talin-1 · UniProt Q9Y490

Round 2 corrected
Length
2541 aa
Mass
269.8 kDa
Annotated
2026-04-28
70 papers in source corpus 22 papers cited in narrative 22 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TLN1 encodes talin-1, a large cytoskeletal adaptor protein that serves as a central mechanical and signaling hub at integrin-based focal adhesions, coupling integrin activation to the actin cytoskeleton and transducing force-dependent biochemical signals. Its N-terminal FERM/F3 (PTB-like) subdomain directly binds integrin β cytoplasmic tails via NPXY motifs to activate integrins such as αIIbβ3, a process regulated by RAP1 GTPase binding, while its C-terminal I/LWEQ module binds and stabilizes F-actin, dimerizes via a coiled-coil motif, and targets the protein to focal adhesions through a mechanism subject to intrasteric autoinhibition (PMID:11932255, PMID:9159132, PMID:15581353, PMID:16830345). Talin-1 recruits vinculin and FAK to focal adhesions and activates FAK/AKT/ERK signaling; its mechanosensitive R1–R2 domains contain a cancer-enriched alternatively spliced exon (exon 17b, regulated by TGF-β/SMAD3) that lowers the force threshold for vinculin recruitment (PMID:7622520, PMID:18056416, PMID:36880935). TLN1 protein stability is post-translationally controlled by competition between EMP1 and the E3 ubiquitin ligase SMURF1, while its transcription is cooperatively regulated by FLI1 and GATA1; germline FLI1 variants that impair this cooperative transcription reduce talin-1 levels in platelets and contribute to bleeding disorders (PMID:41284206, PMID:39744817).

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 1995 High

    Establishing a direct physical link between talin and FAK answered how integrin engagement triggers kinase activation at focal adhesions, positioning talin as a scaffold that recruits signaling enzymes.

    Evidence Co-immunoprecipitation and in vitro binding with FAK C-terminal deletion mutants in NIH 3T3 cells

    PMID:7622520

    Open questions at the time
    • Structural basis of the 48-residue FAK-binding site on talin was not resolved
    • Whether talin is sufficient or merely required for FAK activation was not tested
  2. 1997 High

    Identification of the C-terminal I/LWEQ module as an F-actin binding domain resolved how talin physically connects to the actin cytoskeleton, completing the conceptual integrin–talin–actin linkage.

    Evidence In vitro F-actin competition binding assay with mutagenesis of conserved residues across talin and yeast Sla2

    PMID:10581178 PMID:9159132

    Open questions at the time
    • Affinity and stoichiometry of actin binding were not quantified
    • In vivo relevance of the I/LWEQ module in mammalian cells was not yet shown
  3. 2002 High

    Demonstrating that the talin head F3 subdomain activates integrins via PTB-like recognition of β-tail NPXY motifs resolved the molecular basis of inside-out integrin signaling and identified talin as a direct integrin activator.

    Evidence Subdomain binding assays, cell-based αIIbβ3 activation, NPXY mutagenesis, and molecular modeling

    PMID:11932255 PMID:12606711

    Open questions at the time
    • Structural determination of the F3–integrin tail complex was pending
    • Relative contributions of F2 versus F3 in full-length talin context were unclear
  4. 2002 High

    Discovery that PIPKIγ661 is recruited to focal adhesions through direct talin binding—enhanced by FAK-dependent phosphorylation—established a mechanism for spatially restricted PtdIns(4,5)P₂ production at adhesion sites.

    Evidence Reciprocal co-immunoprecipitation, subcellular fractionation, in vitro kinase assay in FAK-null and wild-type cells

    PMID:12422220

    Open questions at the time
    • Whether PIPKIγ binding and integrin-tail binding on talin are mutually exclusive was not resolved
    • PIP₂ levels at individual focal adhesions were not measured
  5. 2004 High

    Revealing intrasteric autoinhibition of the I/LWEQ module explained how talin's actin-binding and focal adhesion targeting activities are kept latent until activation, providing a regulatory framework for the protein's cytoskeletal functions.

    Evidence Truncation analysis, in vitro F-actin cosedimentation, and binding affinity measurements across talin1, talin2, Hip1, and Hip12

    PMID:15581353 PMID:16830345

    Open questions at the time
    • The signal or event that relieves autoinhibition in cells was not identified
    • Structural basis of the inhibitory α-helix interaction was not solved
  6. 2007 High

    Alanine-scanning mutagenesis of the I/LWEQ coiled-coil showed that dimerization is essential for F-actin binding, filament cross-linking, and focal adhesion targeting, unifying these activities under a single structural requirement.

    Evidence Alanine-scanning mutagenesis combined with in vitro cosedimentation and GFP-fusion focal adhesion targeting in cells

    PMID:17722883

    Open questions at the time
    • Crystal structure of the dimerized I/LWEQ module was not available
    • Whether talin forms homodimers or heterodimers with talin-2 in vivo was untested
  7. 2007 High

    Detailed analysis of vinculin–talin interaction showed that vinculin head binding to talin controls integrin dynamics and clustering while the vinculin tail couples adhesions to actin force, establishing a cooperative talin–vinculin mechanotransduction unit.

    Evidence Vinculin mutant expression, FRAP dynamics of focal adhesions, co-immunoprecipitation

    PMID:18056416

    Open questions at the time
    • Force-dependent exposure of vinculin-binding sites on talin was not directly measured in this study
    • Stoichiometry of vinculin per talin molecule at adhesions was not determined
  8. 2023 High

    Discovery of cancer-enriched exon 17b insertion between talin R1–R2 domains, regulated by TGF-β/SMAD3, revealed that alternative splicing tunes the force threshold for vinculin recruitment, linking transcriptional regulation to mechanosensitive adhesion properties.

    Evidence Single-molecule magnetic tweezers force spectroscopy, biochemical vinculin binding, live-cell motility imaging, TGF-β/SMAD3 manipulation

    PMID:36880935

    Open questions at the time
    • In vivo relevance of exon 17b for tumor metastasis was not tested in animal models
    • Whether other R-domain force switches are similarly spliced is unknown
  9. 2025 High

    Identification of cooperative FLI1/GATA1 transcriptional regulation of TLN1 through an intronic enhancer, with FLI1 germline variants causing up to 88% reduction in platelet talin-1, connected TLN1 transcriptional control to inherited bleeding disorders.

    Evidence scRNA-seq, luciferase reporters, ChIP-seq, Western blot quantification of patient platelet talin-1

    PMID:39744817

    Open questions at the time
    • Whether talin-2 compensates for talin-1 loss in patient platelets was not addressed
    • The specific causal mutation–phenotype relationship in a full pedigree was not fully shown
  10. 2025 Medium

    Demonstrating that EMP1 stabilizes talin-1 by competing with SMURF1-mediated ubiquitination revealed a post-translational mechanism controlling TLN1 protein levels, linking this to FAK phosphorylation and hepatic stellate cell activation.

    Evidence Co-immunoprecipitation, ubiquitination assay, siRNA knockdown in hepatic stellate cells, rodent MASLD-IRI model

    PMID:41284206

    Open questions at the time
    • The specific ubiquitination sites on talin-1 targeted by SMURF1 were not mapped
    • Whether this degradation pathway operates in non-hepatic contexts is unknown
    • Single-lab finding awaits independent replication

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis of full-length talin autoinhibition and activation, the in vivo significance of talin dimerization, and whether the exon 17b splice isoform is required for metastasis in animal cancer models.
  • Full-length talin structure in autoinhibited versus activated state not determined
  • In vivo functional consequences of talin homo- versus heterodimerization (talin-1/talin-2) are uncharacterized
  • Therapeutic targeting of the TLN1–integrin interface (e.g., C67399) lacks clinical validation

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 5 GO:0060090 molecular adaptor activity 5 GO:0005198 structural molecule activity 3
Localization
GO:0005856 cytoskeleton 6 GO:0005886 plasma membrane 3
Pathway
R-HSA-162582 Signal Transduction 5 R-HSA-1500931 Cell-Cell communication 4 R-HSA-1474244 Extracellular matrix organization 3 R-HSA-109582 Hemostasis 2
Partners
ITGB1ITGB3LAYNPIP5K1CPTK2RAP1ASMURF1VCL
Complex memberships
Integrin–talin–vinculin focal adhesion complex

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 The conserved C-terminal I/LWEQ module (last ~197 amino acids) of vertebrate talin (TLN1) is an F-actin binding domain; the corresponding domain from yeast Sla2 also binds F-actin in vitro, and mutation of a conserved residue in the fourth block abolishes this interaction, defining the I/LWEQ module as a new actin-binding motif. In vitro F-actin competition binding assay, site-directed mutagenesis, sequence analysis Proceedings of the National Academy of Sciences of the United States of America High 9159132
1999 The I/LWEQ modules from all four major groups (metazoan talin, Dictyostelium TalA/B, metazoan Hip1, yeast Sla2) bind F-actin in vitro, and GFP-fusion proteins of the I/LWEQ modules of talin and Sla2 bind F-actin in vivo, confirming the I/LWEQ module as a conserved actin-binding motif linking diverse proteins to the actin cytoskeleton. In vitro F-actin binding assay, GFP-fusion localization in cells Biochemical and biophysical research communications High 10581178
1999 The human TLN1 gene spans more than 23 kb, consists of 57 exons, and maps to chromosome band 9p13; Northern blot analysis showed TLN expression in diverse human tissues including leukocytes, lung, placenta, liver, kidney, spleen, thymus, colon, skeletal muscle, and heart. BAC clone isolation, FISH, radiation hybrid mapping, Northern blot Genomics Medium 10610730
2002 The talin head domain F3 subdomain (PTB-like) binds the integrin β3 cytoplasmic tail with higher affinity than F2 and activates integrin αIIbβ3 when expressed in cells; this interaction depends on NPXY motifs in the integrin β tail, establishing that talin activates integrins through a PTB domain–like mechanism. Binding assays with isolated subdomains, cell-based integrin activation assay, molecular modeling, mutagenesis The Journal of biological chemistry High 11932255
2002 Type I phosphatidylinositol phosphate kinase isoform-γ661 (PIPKIγ661) is targeted to focal adhesions through direct association with talin; PIPKIγ661 is tyrosine-phosphorylated by FAK signaling, which increases both its kinase activity and its association with talin, defining a mechanism for spatially restricted PtdIns(4,5)P2 generation at focal adhesions. Co-immunoprecipitation, subcellular fractionation, in vitro kinase assay, FAK mutant cells Nature High 12422220
2003 Multiple phosphotyrosine-binding (PTB) domain-containing proteins (including talin, ICAP1-α, Numb, Dok-1) bind integrin β cytoplasmic tails via a canonical PTB domain–NPXY ligand interaction; integrin class-specific interactions were demonstrated, confirming talin's PTB-like F3 subdomain as a conserved integrin-tail binding module. Recombinant PTB domain binding assays, co-immunoprecipitation, NPXY mutagenesis, gain/loss-of-function mutations Proceedings of the National Academy of Sciences of the United States of America High 12606711
2004 The actin-binding activity of Talin1 (and Talin2, Hip1, Hip12) within the I/LWEQ module is regulated by intrasteric inhibition: a structural element upstream of the I/LWEQ module occludes the primary actin-binding determinants. The I/LWEQ module also contains a dimerization motif and stabilizes actin filaments against depolymerization. In vitro F-actin cosedimentation assay, binding affinity measurements, truncation analysis Biochemistry High 15581353
1995 Focal adhesion kinase (FAK) physically associates with talin (TLN1) in NIH 3T3 cells; a 48-amino acid sequence in the FAK C-terminal domain mediates talin binding in vitro, and integrin-induced FAK phosphorylation correlates with integrin's ability to bind talin, suggesting talin mediates FAK activation downstream of integrins. Co-immunoprecipitation, in vitro binding assay with FAK deletion mutants, integrin mutant analysis The Journal of biological chemistry High 7622520
2006 The I/LWEQ module at the C-terminus of Talin1 is necessary and sufficient for targeting to focal adhesion complexes in vivo; this focal adhesion targeting function correlates with but is separable from actin binding, and is regulated by an intrasteric interaction with an upstream α-helix that masks both the actin-binding and adhesion-targeting elements in full-length Talin1. GFP-fusion truncation constructs, live-cell imaging, site-directed mutagenesis Cell motility and the cytoskeleton High 16830345
2007 The C-terminal dimerization motif (a putative coiled coil) within the Talin1 I/LWEQ module is required for F-actin binding, actin filament stabilization, actin filament cross-linking, and focal adhesion targeting; alanine-scanning mutagenesis of this coiled coil abolishes all these activities, demonstrating that dimerization is essential for Talin1 function at focal adhesions. Alanine-scanning mutagenesis, in vitro F-actin cosedimentation, GFP-fusion focal adhesion targeting assay Biochemistry High 17722883
2007 Vinculin directly interacts with talin (TLN1) to regulate focal adhesion formation; the vinculin head (binding talin) controls integrin dynamics and clustering while the vinculin tail links focal adhesions to the actin force machinery; expression of vinculin constructs with unmasked binding sites induces dramatic focal adhesion growth via direct talin interaction. Vinculin mutant expression, FRAP, co-immunoprecipitation, quantitative FA analysis The Journal of cell biology High 18056416
2013 TLN1 loss-of-function (shRNA knockdown) significantly enhances docetaxel chemosensitivity specifically in triple-negative breast cancer (TNBC) cell lines but not in hormone receptor-positive or normal mammary epithelial cells; TLN1-deficient xenografts show reduced tumor mass after chemotherapy, establishing TLN1 as a modulator of chemotherapy response in TNBC. shRNA RNAi screen, cell viability assays in multiple cell lines, mouse xenograft model Clinical cancer research Medium 23479679
2013 TLN1 is a direct target of miR-9 in ovarian serous carcinoma cells; TLN1 knockdown mimics miR-9 overexpression (inhibiting proliferation, migration, invasion), and TLN1 modulates the FAK/AKT signaling pathway, which is inhibited by increased miR-9 levels. Luciferase reporter assay (3'UTR targeting), siRNA knockdown, miRNA transfection, Western blot for FAK/AKT pathway International journal of molecular medicine Medium 23722670
2021 TLN1 and FAK form a functional axis (Cdk5/Tln1/FAK) required for cancer cell trans-endothelial migration (TEM) and extravasation; the structural (not phosphorylation-dependent) functions of FAK and Tln1 are required for invadopodia formation and vascular breaching; inhibition of FAKS732 phosphorylation delocalizes ERK from the nucleus and decreases ERK phosphorylation. 3D microfluidic vascular model, siRNA knockdown, chemical inhibition, in vivo lung colonization assay, immunofluorescence, Western blot Biomaterials Medium 34333365
2022 TLN1 interacts with integrin β1 (ITGB1) at focal adhesions; silencing TLN1 in TNBC cells attenuates migration by interfering with dynamic focal adhesion formation and regulates the FAK-AKT signaling pathway and epithelial-mesenchymal transition; a small molecule C67399 blocking the TLN1–integrin β1 binding interface suppresses TNBC metastasis in xenograft models. Western blot, RT-PCR, co-immunoprecipitation, computational drug screening targeting protein-protein interface, xenograft assay, siRNA knockdown eLife Medium 35285795
2023 TLN1 contains a cancer-enriched alternatively spliced exon (exon 17b, 51 nt) inserted between exons 17 and 18 that introduces 17 amino acids between the R1 and R2 force-dependent switch domains; this lowers the force required to open R1-R2 switches, enhances vinculin binding, and alters cell adhesion dynamics and motility; TGF-β/SMAD3 signaling regulates this isoform switch. Alternative splicing analysis, single-molecule force spectroscopy (magnetic tweezers), biochemical vinculin binding assay, live-cell adhesion/motility imaging, TGF-β/SMAD3 pathway manipulation The Journal of cell biology High 36880935
2023 In C. elegans, TLN-1 W387A (CRISPR-generated allele predicted to disrupt integrin binding) produces only mild motility and ovulation defects without disrupting actin cytoskeleton architecture in body wall muscles or gonadal sheath, suggesting that W387 plays a minor role in integrin binding in vivo. CRISPR allele generation, behavioral assays, fluorescence microscopy of actin cytoskeleton microPublication biology Medium 36685729
2023 TLN1 overexpression in cardiac microvascular endothelial cells (CMVECs) upregulates integrin alpha 5 (ITGA5), and ITGA5 knockdown reverses the protective effects of TLN1 overexpression against ox-LDL-induced dysfunction, establishing a TLN1→ITGA5 axis in endothelial cell adhesion and survival. Overexpression and siRNA knockdown in CMVECs, CCK-8 proliferation assay, flow cytometry apoptosis, angiogenesis assay, Western blot Folia morphologica Low 37144848
2025 FLI1 and GATA1 cooperatively regulate TLN1 transcription through an intronic FLI1-binding region; FLI1 germline variants associated with bleeding disorders show reduced cooperative transcriptional activity, resulting in up to 88% reduction in talin-1 protein levels in patient platelets, contributing to platelet dysfunction. scRNA-seq, luciferase reporter assay, ChIP-seq analysis, Western blot of patient platelets, FLI1 variant characterization Haematologica High 39744817
2025 EMP1 competes with SMURF1 for the TLN1 binding site, thereby inhibiting SMURF1-mediated ubiquitination and degradation of TLN1; accumulated TLN1 promotes FAK phosphorylation, establishing an EMP1/TLN1/FAK pro-inflammatory signaling axis that drives hepatic stellate cell activation; EMP1 silencing suppresses this post-translational cascade. Co-immunoprecipitation, ubiquitination assay, siRNA knockdown, Western blot, rodent MASLD-IRI model, human sample validation Molecular biomedicine Medium 41284206
2025 RAP1 GTPase regulates platelet aggregation by direct interaction with TALIN-1 (Tln-1) to control αIIbβ3 integrin activation; mice with impaired RAP1–Tln-1 interaction (Tln-1 mR35/118E) show a milder defect in phosphatidylserine exposure than complete Rap1 knockout, and ROCK inhibition fully restores PtdSer exposure in Tln-1 mR35/118E platelets, establishing that RAP1 regulates procoagulant activity through both Tln-1-dependent (integrin) and -independent (RHOA) pathways. Knock-in mouse model (Tln-1 mR35/118E), flow cytometry for PtdSer exposure, ROCK inhibitor pharmacology, in vitro and in vivo coagulation assays bioRxivpreprint Medium bio_10.1101_2025.05.22.655614
2024 LAYN (layilin) interacts with TLN1 (validated by co-immunoprecipitation), and CREB1 binds the LAYN promoter to transcriptionally upregulate LAYN; the resulting CREB1→LAYN→TLN1→integrin β1 (ITGB1) signaling axis promotes cholangiocarcinoma metastasis by activating c-Jun, p38 MAPK, and ERK phosphorylation. Co-immunoprecipitation, ChIP, luciferase reporter, overexpression/knockdown, Western blot, in vivo metastasis model Heliyon Medium 39286102

Source papers

Stage 0 corpus · 70 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2004 Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nature biotechnology 916 15592455
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
1992 Cytoskeleton--plasma membrane interactions. Science (New York, N.Y.) 707 1439807
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2007 Vinculin controls focal adhesion formation by direct interactions with talin and actin. The Journal of cell biology 678 18056416
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2003 Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions. Blood 616 14630798
2008 Large-scale proteomics and phosphoproteomics of urinary exosomes. Journal of the American Society of Nephrology : JASN 607 19056867
2005 A quantitative protein interaction network for the ErbB receptors using protein microarrays. Nature 568 16273093
2011 Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation. Nature cell biology 490 21423176
2003 Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nature biotechnology 485 12665801
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2010 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.) 421 20360068
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2000 Interactions of the low density lipoprotein receptor gene family with cytosolic adaptor and scaffold proteins suggest diverse biological functions in cellular communication and signal transduction. The Journal of biological chemistry 402 10827173
2002 Type I gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions. Nature 396 12422220
2003 Integrin beta cytoplasmic domain interactions with phosphotyrosine-binding domains: a structural prototype for diversity in integrin signaling. Proceedings of the National Academy of Sciences of the United States of America 341 12606711
1995 Interaction of focal adhesion kinase with cytoskeletal protein talin. The Journal of biological chemistry 331 7622520
2003 Smooth muscle contraction and relaxation. Advances in physiology education 322 14627618
2002 The phosphotyrosine binding-like domain of talin activates integrins. The Journal of biological chemistry 315 11932255
2016 Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics. Cell reports 306 27342126
2012 A high-throughput approach for measuring temporal changes in the interactome. Nature methods 273 22863883
1995 Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL. The Journal of biological chemistry 272 7534286
1997 The I/LWEQ module: a conserved sequence that signifies F-actin binding in functionally diverse proteins from yeast to mammals. Proceedings of the National Academy of Sciences of the United States of America 149 9159132
2004 Intrasteric inhibition mediates the interaction of the I/LWEQ module proteins Talin1, Talin2, Hip1, and Hip12 with actin. Biochemistry 67 15581353
2013 A targeted RNAi screen of the breast cancer genome identifies KIF14 and TLN1 as genes that modulate docetaxel chemosensitivity in triple-negative breast cancer. Clinical cancer research : an official journal of the American Association for Cancer Research 61 23479679
2013 miR-9 functions as a tumor suppressor in ovarian serous carcinoma by targeting TLN1. International journal of molecular medicine 59 23722670
2019 Rare Missense Variants in TLN1 Are Associated With Familial and Sporadic Spontaneous Coronary Artery Dissection. Circulation. Genomic and precision medicine 48 30888838
1999 Functional genomic analysis reveals the utility of the I/LWEQ module as a predictor of protein:actin interaction. Biochemical and biophysical research communications 45 10581178
2007 A C-terminal dimerization motif is required for focal adhesion targeting of Talin1 and the interaction of the Talin1 I/LWEQ module with F-actin. Biochemistry 41 17722883
2011 A phase II study of the Ras-MAPK signaling pathway inhibitor TLN-4601 in patients with glioblastoma at first progression. Journal of neuro-oncology 36 22048878
2020 Silk fibroin hydrogel promote burn wound healing through regulating TLN1 expression and affecting cell adhesion and migration. Journal of materials science. Materials in medicine 26 32405818
2019 miR-429 suppresses cell proliferation, migration and invasion in nasopharyngeal carcinoma by downregulation of TLN1. Cancer cell international 26 31068760
2020 Tanshinone IIA Suppresses Glioma Cell Proliferation, Migration and Invasion Both in vitro and in vivo Partially Through miR-16-5p/Talin-1 (TLN1) Axis. Cancer management and research 24 33192091
2022 Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer. eLife 23 35285795
2006 The conserved C-terminal I/LWEQ module targets Talin1 to focal adhesions. Cell motility and the cytoskeleton 22 16830345
2016 TLN-58, an Additional hCAP18 Processing Form, Found in the Lesion Vesicle of Palmoplantar Pustulosis in the Skin. The Journal of investigative dermatology 20 27771329
2021 The driving role of the Cdk5/Tln1/FAKS732 axis in cancer cell extravasation dissected by human vascularized microfluidic models. Biomaterials 19 34333365
2010 TLN-4601, a novel anticancer agent, inhibits Ras signaling post Ras prenylation and before MEK activation. Anti-cancer drugs 18 20220516
2022 CircRNA_400029 promotes the aggressive behaviors of cervical cancer by regulation of miR-1285-3p/TLN1 axis. Journal of Cancer 15 35069901
2010 TLN-4601 suppresses growth and induces apoptosis of pancreatic carcinoma cells through inhibition of Ras-ERK MAPK signaling. Journal of molecular signaling 14 21044336
1999 Characterization of the human talin (TLN) gene: genomic structure, chromosomal localization, and expression pattern. Genomics 14 10610730
2014 Copy number alterations and neoplasia-specific mutations in MELK, PDCD1LG2, TLN1, and PAX5 at 9p in different neoplasias. Genes, chromosomes & cancer 13 24664538
2024 TLN1: an oncogene associated with tumorigenesis and progression. Discover oncology 11 39589610
2009 TLN-05220, TLN-05223, new Echinosporamicin-type antibiotics, and proposed revision of the structure of bravomicins(*). The Journal of antibiotics 11 19680283
2003 Interactions of a new alpha-aminophosphinic derivative inside the active site of TLN (thermolysin): a model for zinc-metalloendopeptidase inhibition. Acta crystallographica. Section D, Biological crystallography 11 12832763
2010 TLN-4601 peripheral benzodiazepine receptor (PBR/TSPO) binding properties do not mediate apoptosis but confer tumor-specific accumulation. Biochemical pharmacology 10 20655882
2022 Micro RNA miR-1303 Promotion of Proliferation, Migration and Invasion of Human Liver Cancer Cells Is Enhanced by Low Talin 1 (TLN1) Expression. Anticancer research 8 36192016
2023 TLN1 contains a cancer-associated cassette exon that alters talin-1 mechanosensitivity. The Journal of cell biology 7 36880935
2023 TLN1 synergizes with ITGA5 to ameliorate cardiac microvascular endothelial cell dysfunction. Folia morphologica 7 37144848
2014 Treatment of Non-transplant patients with multiple myeloma: routine treatment by office-based haematologists in Germany--data from the prospective Tumour Registry Lymphatic Neoplasms (TLN). Oncology research and treatment 7 25486127
2014 The I/LWEQ domain in RapGAP3 required for posterior localization in migrating cells. Molecules and cells 3 24608804
2024 CREB1 promotes cholangiocarcinoma metastasis through transcriptional regulation of the LAYN-mediated TLN1/β1 integrin axis: CREB1 promotes cholangiocarcinoma metastasis through regulating LAYN/TLN1/β1 integrin axis. Heliyon 2 39286102
2023 An integrin binding motif in TLN-1/talin plays a minor role in motility and ovulation. microPublication biology 2 36685729
2025 FLI1 and GATA1 govern TLN1 transcription: new insights into FLI1-related platelet disorders. Haematologica 1 39744817
2025 Genome-wide screening and validation of exosome-derived TLN1 as a regulator of epithelial-mesenchymal transition in lung cancer. Scientific reports 1 40181045
2025 Epithelial membrane protein 1 drives hepatic stellate cell activation via the TLN1/FAK cascade in MASLD donor liver transplantation. Molecular biomedicine 1 41284206
2023 Integrated proteome and malonylome analyses reveal the potential meaning of TLN1 and ACTB in end-stage renal disease. Proteome science 1 37833721
2017 Minimal amino acids in the I/LWEQ domain required for anterior/posterior localization in Dictyostelium. Journal of microbiology (Seoul, Korea) 1 28124777
2002 The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate. Acta crystallographica. Section D, Biological crystallography 1 12454500
2026 Integrated Metabolomic and Genomic Insights into Amino Acid Incorporation Within the Hybrid Polyketide-Alkaloid Antibiotic TLN-05220. ACS chemical biology 0 41614682
2025 Tandem Mass Tag-Labeling Proteomics Reveals TLN1 as a Potential Factor in Cardiogenic Pulmonary Edema. International heart journal 0 40159367
2025 Integrated metabolomic and genomic insights into amino acid incorporation within the hybrid polyketide-alkaloid antibiotic TLN-05220. bioRxiv : the preprint server for biology 0 41279854