Affinage

CDC20

Cell division cycle protein 20 homolog · UniProt Q12834

Length
499 aa
Mass
54.7 kDa
Annotated
2026-04-28
100 papers in source corpus 52 papers cited in narrative 52 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CDC20 is the essential mitotic co-activator of the anaphase-promoting complex/cyclosome (APC/C) E3 ubiquitin ligase, coupling spindle checkpoint signaling to cell cycle progression and extending ubiquitin-dependent regulation to neuronal development, ciliary dynamics, Wnt signaling, and osteogenesis. CDC20 directly binds mitotically phosphorylated APC/C via its C-box and activates D-box- and KEN-box-dependent substrate ubiquitination through a C-terminal WD40 β-propeller that harbors preformed degron-recognition surfaces, targeting cyclin B1, securin, and numerous other substrates for proteasomal destruction (PMID:9734353, PMID:23091007, PMID:11562349). The spindle assembly checkpoint inhibits APC/C(CDC20) through a tripartite mechanism: Mad2 competes with APC/C for the same CDC20 binding site, the MCC (BubR1-Bub3-Mad2-CDC20) provides pseudosubstrate inhibition, and Bub1–Plk1/Mps1-dependent phosphorylation of CDC20 directly blocks APC/C activation—while PP2A-B56 and PP1 counteract inhibitory CDK phosphorylation of CDC20's N-terminus to promote APC/C loading (PMID:23007648, PMID:26912231, PMID:22713866, PMID:36289199). Beyond mitosis, APC/C(CDC20) degrades NeuroD2 to drive presynaptic differentiation, operates at the basal body to promote ciliary resorption via Nek1 degradation, targets conductin to modulate Wnt/β-catenin signaling, ubiquitinates p65/NF-κB to promote osteogenesis, and degrades the pro-apoptotic factor Bim and the pyroptosis effector GSDME (PMID:19900895, PMID:25139956, PMID:22322943, PMID:34382737, PMID:24871945, PMID:37528490).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1997 Medium

    Establishing CDC20 as a cell-cycle-regulated mitotic protein: before its enzymatic role was known, the observation that CDC20 protein levels fluctuate across the cell cycle and that it is degraded via the proteasome at M/G1 set the stage for understanding its mitosis-specific function.

    Evidence Cell cycle synchronization, co-IP, immunolocalization, and proteasome inhibition in HeLa cells

    PMID:9353311

    Open questions at the time
    • Functional consequence of cell-cycle-dependent associated proteins not identified
    • Mechanism of proteasomal degradation not defined
  2. 1998 High

    Identifying CDC20 as the direct mitotic activator of APC/C and the direct target of spindle checkpoint proteins resolved two fundamental questions: what activates APC/C in mitosis and how does the checkpoint inhibit it. CDC20 confers D-box-dependent substrate recognition and its activity is inhibited by MAD2 binding in a ternary MAD2-CDC20-APC complex.

    Evidence In vitro reconstitution of APC activation (Xenopus, human), yeast two-hybrid and co-IP of Mad1/Mad2/Mad3-Cdc20 interactions, checkpoint-resistant Cdc20 mutants in yeast

    PMID:9461437 PMID:9637688 PMID:9734353

    Open questions at the time
    • Structural basis of CDC20–APC/C interaction unknown
    • Mechanism by which Mad2 inhibits CDC20 (competitive vs. conformational) unresolved
  3. 1998 High

    Demonstrating that CDC20 is itself an APC substrate established the autoregulatory feedback that terminates CDC20 activity, although the degradation mechanism proved D-box-independent.

    Evidence Genetic analysis of yeast cdc23/cdc27 mutants with D-box mutant Cdc20

    PMID:9651679

    Open questions at the time
    • Degron mediating D-box-independent APC-dependent CDC20 degradation unidentified
    • Distinction between cis- and trans-ubiquitination not yet made
  4. 1999 High

    Establishing that APC/C must be phosphorylated by Cdk1/cyclin B before CDC20 can bind and activate it explained the cell-cycle timing of APC/C(CDC20) activation and why interphase APC/C is refractory to CDC20.

    Evidence Cell-free clam embryo system with kinase/phosphatase treatment and ubiquitination assay; confirmed independently with human APC/C in vitro

    PMID:10381365 PMID:10793135

    Open questions at the time
    • Specific APC/C phosphorylation sites required for CDC20 binding not mapped
    • Whether CDC20 phosphorylation has additional regulatory roles unclear
  5. 2000 High

    Structural determination of the Mad2–CDC20 complex revealed that a 40-residue segment N-terminal to CDC20's WD40 domain is the minimal Mad2-binding region, and that Mad2's C-terminal region folds upon CDC20 binding, providing the first structural framework for checkpoint inhibition.

    Evidence NMR structure of Mad2, deletion mutagenesis, NMR titration of Mad2–Cdc20 interaction

    PMID:10700282

    Open questions at the time
    • Full-length CDC20 structure unavailable
    • How Mad2 conformational change translates to APC/C inhibition not resolved
  6. 2001 High

    Demonstrating that CDC20 directly binds substrates via its N-terminus independently of APC/C, and that substrate specificity is dictated by the N-terminal region (shown by chimera swaps), established CDC20 as both the substrate receptor and allosteric activator of APC/C.

    Evidence In vitro binding assays without APC, Cdc20/Cdh1 chimeric protein construction and functional testing; D-box-dependent Pds1–Cdc20 binding in yeast

    PMID:11553328 PMID:11562349

    Open questions at the time
    • D-box vs. KEN-box recognition surfaces on CDC20 not structurally resolved
    • Relative contributions of APC/C-bound vs. free CDC20 in substrate recruitment unknown
  7. 2001 High

    Establishing that Mad2 forms mutually exclusive complexes with Mad1 and CDC20, with Mad1 required for kinetochore recruitment of Mad2, provided the template model for kinetochore-catalyzed checkpoint complex assembly.

    Evidence Co-IP, kinetochore imaging, monomeric Mad2 point mutant functional analysis

    PMID:11707408 PMID:11726501

    Open questions at the time
    • Catalytic mechanism of Mad2 conversion (open to closed) at kinetochores not defined
    • Role of Bub3 WD40 repeats in scaffold function partially characterized
  8. 2003 High

    Mapping CDC20 phosphorylation sites (Ser50, Thr64, Thr68, Thr79) and showing that phosphorylation-deficient CDC20 cannot respond to the spindle checkpoint due to reduced affinity for BubR1/Bub3/Mad2 revealed that CDC20 phosphorylation is required for checkpoint complex formation.

    Evidence In vitro kinase assays and phosphomutant analysis in Xenopus egg extracts

    PMID:12855955

    Open questions at the time
    • Kinase(s) responsible for each site not fully assigned
    • Whether phosphorylation regulates CDC20 conformation or merely electrostatic affinity unclear
  9. 2007 High

    Genetic ablation of Cdc20 in mice caused two-cell-stage metaphase arrest with high cyclin B1, and this arrest was securin-dependent, proving CDC20 is non-redundant for mammalian mitosis and placing securin as a key downstream substrate.

    Evidence Gene trap mouse knockout and Cdc20/securin double mutant embryo phenotyping

    PMID:17325031

    Open questions at the time
    • Contribution of individual substrates (cyclin B vs. securin) to arrest not fully dissected
    • Whether CDC20 has essential roles beyond substrate degradation in mammals unknown
  10. 2009 High

    Discovery that APC/C(CDC20) operates in postmitotic neurons—driving dendrite morphogenesis from the centrosome and presynaptic differentiation via NeuroD2 degradation—fundamentally expanded CDC20 function beyond cell division.

    Evidence Knockdown in cerebellar slices and in vivo postnatal rats; centrosomal localization; NeuroD2-Complexin II epistasis pathway

    PMID:19167333 PMID:19900895

    Open questions at the time
    • Mechanism activating APC/C(CDC20) in postmitotic neurons without mitotic kinases unresolved
    • Full complement of neuronal CDC20 substrates unknown
  11. 2011 High

    Reconstitution of CDC20 autoubiquitination revealed an intramolecular (cis) mechanism occurring while CDC20 is bound to APC/C, independent of the C-box required for trans-substrate ubiquitination, and showed that high substrate levels suppress autoubiquitination—explaining how substrate depletion triggers CDC20 turnover.

    Evidence In vitro ubiquitination assay with C-box mutants and substrate competition experiments

    PMID:22079111

    Open questions at the time
    • Structural basis of cis-ubiquitination geometry not resolved
    • In vivo relevance of substrate-competition model not directly tested
  12. 2012 High

    A cluster of breakthroughs in 2012 resolved the structural basis of CDC20 function and its regulation: the crystal structure revealed preformed D-box and KEN-box binding surfaces on the WD40 β-propeller; Mad2 was shown to compete with APC/C for the same CDC20 binding site; PP2A-mediated dephosphorylation of CDC20's N-terminus was found essential for APC/C loading; and APC15 was identified as the specific subunit enabling MCC-dependent CDC20 autoubiquitylation and checkpoint silencing.

    Evidence X-ray crystallography with mutagenesis (PMID:23091007); in vitro competition binding and in vivo checkpoint mutant (PMID:23007648); Xenopus extract PP2A/phosphomutant reconstitution (PMID:22713866); APC15 knockdown and reconstituted APC/C ubiquitylation (PMID:23007861); APC subunit mutant co-IP with live imaging (PMID:21336306)

    PMID:21336306 PMID:22713866 PMID:23007648 PMID:23007861 PMID:23091007

    Open questions at the time
    • Complete cryo-EM structure of APC/C–MCC–CDC20 ternary complex not yet available at this point
    • How APC15 specifically enables autoubiquitylation mechanistically not fully explained
  13. 2014 High

    Identification of Bim as an APC/C(CDC20) substrate linked CDC20 to apoptosis regulation, while localization of CDC20 to the basal body and its targeting of Nek1 established a role in primary cilium resorption—further diversifying CDC20's non-mitotic functions.

    Evidence siRNA screen with Bim epistasis and cell death assays; immunofluorescence at basal body with cilium length assays and Nek1 ubiquitination

    PMID:24871945 PMID:25139956

    Open questions at the time
    • Mechanism of CDC20 recruitment to basal body unknown
    • Whether APC/C regulation at cilia uses the same phosphorylation switches as mitosis untested
  14. 2016 High

    Two parallel regulatory inputs on CDC20 were clarified: Bub1 directly phosphorylates CDC20 and scaffolds Plk1-mediated phosphorylation to inhibit APC/C independently of MCC, while cyclin A2–Cdk2 phosphorylates CDC20 in interphase to prevent premature APC/C activation—establishing a positive feedback loop for cyclin accumulation.

    Evidence In vitro kinase assays, phosphomutant analysis, APC/C ubiquitination assay, Mad2/BubR1 depletion epistasis; phosphomutant Cdc20 cell cycle analysis

    PMID:26912231 PMID:26960431

    Open questions at the time
    • Relative quantitative contributions of Bub1-Plk1 vs. MCC inhibition not measured
    • How interphase Cdk2-mediated inhibition is relieved at mitotic entry not fully defined
  15. 2016 High

    Identification of SPOP-Cullin3 as an APC-independent E3 ligase system that ubiquitinates and degrades CDC20 revealed that CDC20 stability is controlled by multiple ubiquitin ligase pathways, and that prostate cancer SPOP mutations stabilize CDC20.

    Evidence Co-IP, ubiquitination assay, half-life analysis, SPOP mutant characterization

    PMID:27780719

    Open questions at the time
    • Cell cycle phase specificity of SPOP-CDC20 regulation not defined
    • Whether SPOP-dependent CDC20 regulation operates in normal physiology or is primarily tumor-relevant unclear
  16. 2019 High

    PP2A-B56 was shown to bind APC/C via the Apc1-loop500 and preferentially dephosphorylate CDC20 (rather than Apc1) to promote CDC20 loading, resolving how APC/C-intrinsic phosphatase activity enables CDC20 activation in the presence of high CDK activity.

    Evidence Xenopus egg extract reconstitution, Apc1 loop mutants, phosphomutant CDC20 bypass experiment

    PMID:31825153

    Open questions at the time
    • Whether PP2A-B56 acts on CDC20 before or after its initial APC/C encounter not resolved
    • Structural model of PP2A-B56 docked on APC/C lacking
  17. 2021 High

    Non-mitotic substrate diversity of APC/C(CDC20) was greatly expanded: CDC20 targets p65/NF-κB for degradation to promote osteogenesis (validated by conditional KO rescue), and GSDME degradation by CDC20 controls the switch between apoptosis and pyroptosis with consequences for anti-tumor immunity.

    Evidence Cdc20 conditional KO mice with p65 knockdown rescue for osteogenesis; GSDME ubiquitination assay and syngeneic tumor models with GSDME-dependent immune infiltration

    PMID:34382737 PMID:37528490

    Open questions at the time
    • How CDC20 is activated for p65 degradation in osteoblasts without mitotic signals unclear
    • Whether GSDME degradation by CDC20 is cell-cycle-dependent or constitutive not determined
  18. 2022 High

    The complete kinetochore-catalyzed mechanism for Mad2–CDC20 assembly was structurally resolved: Mps1-phosphorylated Mad1 creates a phospho-specific interaction with CDC20, and together with Bub1, forms a tripartite Bub1–CDC20–Mad1 assembly that positions CDC20's Mad2-interacting motif near open-Mad2 for catalytic conversion.

    Evidence NMR/biochemical structural analysis, crosslinking mass spectrometry, phosphorylation assays, mutagenesis

    PMID:33384372 PMID:36289199

    Open questions at the time
    • Full reconstitution of kinetochore-catalyzed MCC assembly with all components not achieved
    • Kinetic parameters of the catalytic conversion in cells not measured

Open questions

Synthesis pass · forward-looking unresolved questions
  • Several mechanistic questions remain open: how APC/C(CDC20) is activated in postmitotic or non-dividing cell contexts (neurons, osteoblasts, ciliated cells) without canonical mitotic kinase signaling; the full complement of CDC20 substrates beyond mitosis; and the structural basis for how APC15 specifically enables MCC-dependent autoubiquitylation.
  • Activation mechanism of APC/C(CDC20) in postmitotic cells uncharacterized
  • Systematic identification of non-mitotic CDC20 substrates lacking
  • Complete structural model of APC/C–MCC with APC15 explaining autoubiquitylation mechanism not available

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 5 GO:0098772 molecular function regulator activity 4
Localization
GO:0005815 microtubule organizing center 3 GO:0005694 chromosome 2 GO:0005634 nucleus 1 GO:0005929 cilium 1
Pathway
R-HSA-1640170 Cell Cycle 8 R-HSA-392499 Metabolism of proteins 6 R-HSA-1266738 Developmental Biology 2 R-HSA-162582 Signal Transduction 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-1852241 Organelle biogenesis and maintenance 1
Partners
ANAPC15ANAPC3BUB1BUB1BBUB3MAD1L1MAD2L1RASSF1
Complex memberships
APC/CMCC (Mad2-BubR1-Bub3-Cdc20)

Evidence

Reading pass · 52 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 hMAD2 inhibits APC activation by forming a hMAD2-CDC20-APC ternary complex; only the tetrameric form of hMAD2 inhibits APC activation, while both tetramer and monomer bind CDC20. Three distinct mitotic APC forms exist: inactive hMAD2-CDC20-APC (metaphase), active CDC20-APC (anaphase), and CDH1-APC (late mitosis/G1). In vitro reconstitution, Xenopus embryo injection, recombinant protein binding assays Genes & development High 9637688
1998 Budding yeast Cdc20 is the direct target of the spindle checkpoint: Mad1, Mad2, and Mad3 interact with Cdc20 (two-hybrid and co-precipitation). Mad2 binding to Cdc20 depends on Mad1; Mad3 binding depends on Mad1 and Mad2. Cdc20 mutants resistant to checkpoint no longer bind Mad proteins. Yeast two-hybrid, co-immunoprecipitation, overexpression and mutant analysis Science High 9461437
1998 hCDC20 and hCDH1 directly bind to APC and activate its cyclin ubiquitination activity. hCDC20 confers strict destruction-box (D-box) dependence; its protein levels and APC binding peak in mitosis. hCDC20 is the mitotic activator of APC directing D-box-dependent substrate degradation. Direct binding assays, in vitro ubiquitination assay, cell cycle synchronization and Western blot Molecular cell High 9734353
1997 p55Cdc (human CDC20) is expressed from G1/S through M phase, is degraded via the 26S proteasome at M/G1 transition, and shows dynamic changes in subcellular localization during mitosis. Immune complexes of p55Cdc show dramatic differences in associated proteins at G2-to-M transition. Cell cycle synchronization, co-immunoprecipitation, immunolocalization, proteasome inhibition The Journal of biological chemistry Medium 9353311
1998 Cdc20 (p55Cdc) is itself a substrate of the APC; its instability during S phase and mitosis depends on APC components Cdc23 and Cdc27 but not on its own destruction box during these phases, revealing a D-box-independent APC-dependent degradation mechanism. Genetic analysis of yeast cdc23 and cdc27 mutants, protein stability assays, destruction box mutant analysis Current biology High 9651679
2000 CDC20 can only bind to and activate the mitotically phosphorylated form of APC in vitro. APC phosphorylation (by mitotic kinases) is required for APC(CDC20) activation, while phosphorylation of CDC20 itself is neither sufficient nor required. CDH1 phosphorylation prevents APC activation by CDH1. In vitro binding and ubiquitination assays with phosphorylated/dephosphorylated APC and CDC20, phosphomutant analysis Molecular biology of the cell High 10793135
2000 Solution structure of human Mad2 determined; Mad2 has a novel three-layered alpha/beta fold. The minimal Mad2-binding region of human Cdc20 is a 40-residue segment N-terminal to the WD40 repeats. Mad2 and Cdc20 form a tight 1:1 heterodimeric complex in which the C-terminal flexible region of Mad2 folds upon Cdc20 binding. NMR structure determination, deletion mutagenesis, NMR titration experiments Nature structural biology High 10700282
2001 Cdc20 and Cdh1 directly bind APC substrates via their N-termini independent of APC. N-terminal chimeras between Cdc20 and Cdh1 confer substrate specificity matching their N-terminus, demonstrating that Cdc20 acts as both substrate recognition and APC-activating module. In vitro binding assays without APC, chimeric protein construction and functional testing Genes & development High 11562349
2001 Mad2 binding to Mad1 is required for kinetochore localization of Mad2, where Mad2 interacts with Cdc20. Mad2 forms incompatible (mutually exclusive) complexes with Mad1 and Cdc20; Mad2 oligomerization is not required for spindle checkpoint activity. Co-immunoprecipitation, kinetochore localization imaging, monomeric point mutant functional analysis The EMBO journal High 11707408
2001 Yeast anaphase inhibitor Pds1 (securin) directly interacts with Cdc20 in a destruction-box-dependent manner; Pds1 does not interact with Cdh1, establishing that the D-box mediates direct Cdc20-substrate interaction. Co-immunoprecipitation, two-hybrid, in vitro binding with D-box mutants Current biology High 11553328
2001 Bub3 associates with Cdc20, Mad2, and Mad3 in a complex; this association is upregulated by checkpoint activation. Bub3 WD40 motifs are required for its interaction with Mad2, Mad3, and Cdc20, and for proper checkpoint response. Bub3 may serve as a scaffold for checkpoint protein interactions. Co-immunoprecipitation, co-fractionation, WD40 point mutant analysis The EMBO journal Medium 11726501
2003 Xenopus Cdc20 is phosphorylated at Ser50, Thr64, Thr68, and Thr79 during mitosis; MAPK contributes to phosphorylation at Thr64/Thr68. Phosphorylation-deficient Cdc20 mutants are unable to respond to the spindle checkpoint due to reduced affinity for spindle checkpoint proteins (BubR1, Bub3, Mad2). In vitro kinase assays, phosphomutant analysis in Xenopus egg extracts, spindle checkpoint response assays Nature cell biology High 12855955
1999 Cyclosome (APC) activation by FZY/Cdc20 requires prior phosphorylation by Cdk1/cyclin B; non-phosphorylated interphase cyclosome is not stimulated by FZY. Phosphatase treatment of mitotic cyclosome prevents FZY stimulation, reversible by Cdk1/cyclin B. Cell-free system from clam embryos, kinase/phosphatase treatment, cyclin ubiquitination assay Biochemical and biophysical research communications High 10381365
2004 RASSF1A interacts with Cdc20, inhibiting APC/C-Cdc20 activity and stabilizing mitotic cyclins. RASSF1A localizes to microtubules in interphase and centrosomes/spindle in mitosis. RNAi depletion of RASSF1A accelerates mitotic cyclin degradation and mitotic progression via premature APC activation; RASSF1A inhibits APC-Cdc20 independently of Mad2 and Emi1. Co-immunoprecipitation, RNAi knockdown, in vitro APC activity assay, immunofluorescence Nature cell biology High 14743218
2002 In Drosophila, Fzy/Cdc20 is concentrated at kinetochores and centrosomes early in mitosis and is responsible for the first phase of cyclin B destruction on the mitotic spindle. Cyclin B containing a D-box mutation cannot be degraded by Fzy/Cdc20 on spindles, establishing D-box dependence for spindle-associated destruction. In vivo localization, D-box mutant cyclin B (GFP fusion), Drosophila syncytial embryo analysis The Journal of cell biology High 12082076
2003 Cks1 is required for transcriptional activation of CDC20 in budding yeast by promoting periodic dissociation of Cdc28/CDK from the CDC20 promoter and recruitment of the proteasome to the CDC20 promoter. Chromatin immunoprecipitation, genetic analysis of cks1 mutants, promoter analysis Nature High 12827207
2007 Mad3 KEN boxes (KEN30 and KEN296, conserved to human BubR1) are required for spindle checkpoint function. KEN30 mutation abolishes MCC formation and stabilizes Cdc20 in mitosis. Mad3 KEN30 mediates interactions regulating proteolytic turnover of both Cdc20 and Mad3. Yeast genetic mutant analysis, protein stability assays, MCC complex analysis by co-immunoprecipitation PloS one High 17406666
2009 Cdc20-APC plays an essential role in dendrite morphogenesis in postmitotic neurons. Cdc20 is enriched at the centrosome in neurons and centrosomal localization is critical for Cdc20-dependent dendrite development. HDAC6 promotes polyubiquitination of Cdc20 and stimulates centrosomal Cdc20-APC activity to drive dendrite differentiation. Knockdown in cerebellar slices and in vivo (postnatal rats), centrosome fractionation, localization imaging, ubiquitination assay Cell High 19167333
2009 Cdc20-APC regulates presynaptic differentiation in postmitotic neurons by triggering degradation of the transcription factor NeuroD2, which suppresses Complexin II expression. This defines a Cdc20-APC ubiquitin signaling pathway governing presynaptic development. Knockdown in primary neurons and rat cerebellar cortex, ubiquitination assay, epistasis with NeuroD2 and Complexin II Science High 19900895
2010 Mps1 kinase regulates CDC20's association with both Mad2 and BubR1 during interphase (before kinetochore appearance). Mps1 inhibition prevents Cdc20 association with Mad2 or BubR1 during interphase and sharply accelerates anaphase onset. Mps1 has two complementary roles: initial cytoplasmic activation of Cdc20 inhibitors and kinetochore-based sustained inhibition. Chemical genetics (analog-sensitive Mps1), MPS1-null cell reconstitution, co-immunoprecipitation The Journal of cell biology High 20624902
2007 Loss of Cdc20 causes metaphase arrest at the two-cell stage in mouse embryos with high cyclin B1, demonstrating essential non-redundant mitotic function. The metaphase arrest is securin-dependent: Cdc20/securin double mutant embryos cannot maintain metaphase arrest, placing securin downstream of Cdc20 in the pathway. Gene trap mouse knockout, genetic epistasis (Cdc20/securin double mutant), embryo phenotyping Molecular and cellular biology High 17325031
2012 APC15 (human APC/C subunit related to yeast Mnd2) is required for APC/C(MCC)-dependent CDC20 autoubiquitylation and degradation, and for timely anaphase initiation. APC15 is dispensable for substrate ubiquitylation by APC/C(CDC20) or APC/C(CDH1), but is specifically required for MCC disassembly via CDC20 autoubiquitylation. APC15 knockdown, recombinant human APC/C reconstitution, ubiquitylation assays, live-cell imaging Nature structural & molecular biology High 23007861
2012 Crystal structure of human Cdc20 determined alone and bound to a BubR1 KEN box. Cdc20 has a disordered N-terminal region and a C-terminal WD40 β-propeller with a preformed KEN-box-binding site at its top face. A second conserved surface at the side of the β-propeller is identified as a D-box-binding site. Securin D-box, but not KEN box, is critical for securin ubiquitination by APC/C(Cdc20). X-ray crystallography, mutagenesis, in vitro ubiquitination assays Proceedings of the National Academy of Sciences High 23091007
2012 CDC20 binds to different sites on APC/C depending on spindle checkpoint status: requires APC3 and APC8 when SAC is satisfied, but only APC8 when SAC is active. APC10 is crucial for cyclin B1 and securin destruction but not cyclin A. SAC-dependent change in Cdc20 binding site on APC/C alters APC/C substrate specificity. Co-immunoprecipitation with APC/C subunit mutants, live-cell imaging, substrate degradation assays Nature cell biology High 21336306
2012 Mad2 competes with the APC/C for the same binding site on Cdc20; Mad2 inhibits Cdc20 by binding directly to a site required for APC/C binding. Cdc20 mutant that does not stably bind Mad2 abrogates the SAC in vivo, revealing a second mechanism of SAC inhibition beyond pseudosubstrate inhibition. In vitro competition binding assays, Cdc20 mutant characterization, in vivo checkpoint assay The Journal of cell biology High 23007648
2012 Cdc20 is dephosphorylated at conserved threonine residues (T64/T68/T79) by PP2A (active in mitosis); phosphorylation of these residues by CDK inhibits Cdc20 binding to and activation of APC/C. The activation domain of Cdc20 associates with Apc6 and Apc8 subunits. Dephosphorylation of Cdc20 is required for its C-box-dependent APC/C loading. Xenopus egg extract reconstitution, phosphomutant analysis, phosphatase inhibition, co-immunoprecipitation The EMBO journal High 22713866
2011 Cdc20 is degraded by APC through an intramolecular (in cis) autoubiquitination mechanism while bound to its activator-binding site on the APC core, independently of the C-box required for trans-ubiquitination of Cdc20 substrates. High substrate levels reduce Cdc20 autoubiquitination in vitro, linking substrate depletion to Cdc20 turnover. In vitro ubiquitination assay, C-box mutant analysis, substrate competition experiments Current biology High 22079111
2014 APC/C(Cdc20) targets the proapoptotic BH3-only protein Bim for ubiquitination and destruction; Cdc20 depletion sensitizes cells to apoptotic stimuli through Bim accumulation. Identified via siRNA screen showing Cdc20 and APC-core components sensitize cancer cells to chemoradiation in a Bim-dependent manner. siRNA screen, ubiquitination assay, co-immunoprecipitation, Bim genetic rescue, cell death assays Developmental cell High 24871945
2014 APC/C-Cdc20 activity destabilizes axonemal microtubules in the primary cilium. Cdc20 is specifically recruited to the basal body of primary cilia. Inhibition of APC-Cdc20 increases ciliary length; overexpression suppresses cilium formation. APC-Cdc20 promotes ciliary resorption after serum stimulation and targets the ciliary kinase Nek1 for proteolysis. Immunofluorescence localization, overexpression and inhibition of Cdc20, APC activity assays, Nek1 ubiquitination eLife High 25139956
2016 Human checkpoint kinase Bub1 directly phosphorylates Cdc20 and scaffolds Plk1-mediated phosphorylation of Cdc20; this Bub1-Plk1-dependent Cdc20 phosphorylation inhibits APC/C(Cdc20) in vitro and is required for spindle checkpoint signaling in human cells. This mechanism is parallel and non-redundant with MCC formation. In vitro kinase assay, phosphomutant analysis, APC/C ubiquitination assay, mitotic arrest assay with Mad2/BubR1 depletion Nature communications High 26912231
1999 Cdc20 associates with the aurora2/Aik kinase in HeLa cells; Cdc20-associated kinase activity peaks in early M phase (embryonic) or G2 (somatic). Aurora2/Aik localizes at mitotic spindle poles similarly to Cdc20. Co-immunoprecipitation, kinase activity assay Proceedings of the National Academy of Sciences Medium 10377410
2000 p55CDC (human Cdc20) interacts with BubR1; this interaction is confirmed by yeast two-hybrid, GST pulldown, and co-immunoprecipitation. Spindle checkpoint activation by nocodazole enhances the p55CDC-BubR1 association. BUBR1 phosphorylates p55CDC in vitro, suggesting BUBR1 may regulate APC via p55CDC. Yeast two-hybrid, GST pulldown, co-immunoprecipitation, in vitro kinase assay Oncogene Medium 11030144
2004 Speriolin is a novel spermatogenic cell-specific Cdc20-binding protein that co-localizes with Cdc20 at centrosomes of spermatocytes/spermatids. The seven WD40 repeats of Cdc20 are required for speriolin binding and for localization of Cdc20 to centrosomes and nucleus in meiotic cells. Co-immunoprecipitation, WD40 deletion mutant analysis, immunolocalization The Journal of biological chemistry Medium 15280373
2010 Cdc20 is critical for meiosis I in female mice: hypomorphic Cdc20 mice show chromosome lagging and misalignment during meiosis I, inefficient degradation of cyclin B1, cyclin A2, and securin in metaphase I, and markedly delayed anaphase I onset. Hypomorphic mouse model, oocyte cytology, protein level analysis by Western blot PLoS genetics High 20941357
2009 p53 downregulates Cdc20 transcriptionally by directly binding to a consensus p53-binding site in the Cdc20 promoter and inducing chromatin remodeling (histone deacetylation). DNA damage-induced endogenous p53 also uses the CDE/CHR element to suppress Cdc20 in a p21-independent manner. Chromatin immunoprecipitation, promoter reporter assays, p53 binding site mutagenesis Nucleic acids research High 19273532
2012 Conductin/axin2 is degraded by APC/C-CDC20 during mitosis. CDC20 knockdown blocks Wnt/β-catenin signaling through conductin stabilization. CDC20-resistant conductin inhibits Wnt signaling and attenuates colony formation, placing CDC20-mediated conductin degradation as a mechanism for cell cycle-coupled regulation of Wnt signaling. CDC20 knockdown, CDC20-resistant conductin mutant, Wnt/β-catenin reporter assay, cell cycle synchronization EMBO reports Medium 22322943
2015 Parkin E3 ubiquitin ligase interacts with CDC20 and CDH1 to mediate degradation of mitotic regulators independently of APC/C. Parkin is phosphorylated and activated by Plk1 during mitosis. Parkin deficiency leads to mitotic defects and genomic instability through accumulation of CDC20/CDH1-dependent substrates. Co-immunoprecipitation, in vitro ubiquitination assay, Parkin KO phenotype, Plk1 phosphorylation assay Molecular cell High 26387737
2016 SPOP (Cullin3 adaptor protein) directly interacts with Cdc20 via its degron and promotes Cdc20 poly-ubiquitination and degradation. Prostate cancer-derived SPOP mutants fail to interact with Cdc20 and cannot promote its degradation, leading to Cdc20 overexpression. Cullin3 (not Cullin1) specifically interacts with and regulates Cdc20 stability. Co-immunoprecipitation, ubiquitination assay, half-life analysis with cycloheximide, SPOP mutant characterization Cancer letters High 27780719
2010 APC/C(Cdc20) targets E2F1 for degradation in prometaphase. Cdc20 knockdown increases E2F1 levels and stabilizes E2F1 in prometaphase. Co-expression of DP1 with E2F1 blocks APC/C-induced E2F1 degradation. Cdc20 RNAi/siRNA, ectopic expression of APC/C activators, cell synchronization and Western blot Cell cycle Medium 20948288
2010 Cdc20 contributes to post-anaphase (KEN-box-dependent) degradation of CENP-F, which is CDC20-dependent but Cdh1-independent (Cdh1-null cells still degrade CENP-F normally). This challenges the model that APC/C switches entirely to Cdh1 after anaphase onset. RNAi against APC/C subunits and Cdc20, Cdh1 null cells, KEN-box mutant analysis Journal of cell science Medium 20053638
2017 Kinetochore-localized PP1 phosphatase dephosphorylates Cdc20, directing it toward APC/C activation; flux of Cdc20 through the same kinetochore binding site can also promote checkpoint inhibition. Microtubule attachment status controls the balance between these opposing Cdc20 fates. Kinetochore PP1 tethering, Cdc20 phosphomutant analysis, live-cell imaging of Cdc20 dynamics Genes & development High 28698300
2016 Cyclin A2-Cdk2 binds and phosphorylates Cdc20 in interphase, inhibiting APC/C-Cdc20 activity. Preventing Cdc20 phosphorylation causes premature activation of APC/C-Cdc20, destabilizing cyclin B1 and A2, lengthening G2 and slowing mitotic entry. This defines a positive feedback loop: cyclin A2-Cdk2 inhibits APC/C-Cdc20 to allow further cyclin A2 accumulation. In vitro kinase assay, phosphomutant Cdc20 cells, APC/C activity assays, cell cycle analysis Nature communications High 26960431
2019 PP2A-B56 binds to Apc1-loop500 and promotes Cdc20 dephosphorylation and loading to the APC/C in mitosis. PP2A-B56 preferentially dephosphorylates Cdc20 over the Apc1 inhibitory domain. Mutations in Apc1-loop500 that abolish B56 binding decrease Cdc20 loading and APC/C-dependent ubiquitylation; a non-phosphorylatable Cdc20 mutant bypasses this requirement. Xenopus egg extract reconstitution, Apc1 loop mutant analysis, phosphomutant Cdc20, ubiquitylation assay EMBO reports High 31825153
2020 PP1 promotes cyclin B destruction at anaphase onset by removing specific inhibitory phosphorylation in the N-terminus of CDC20. PP1 depletion or inhibition stabilizes cyclin B and delays metaphase-to-anaphase transition; this requirement is lost with CDK1-phosphorylation-defective CDC20-6A mutants. PP1 depletion/inhibition, CDC20-6A phosphomutant cells, live-cell imaging, Western blot Molecular biology of the cell High 32755477
2021 Kinetochore-catalyzed Mad2-Cdc20 assembly operates through a tripartite mechanism: localized delivery of Mad2 and Cdc20 substrates plus two phosphorylation-dependent interactions that geometrically constrain their positions and prime Cdc20 for interaction with Mad2. Kinetochore-specific live-cell probe monitoring Mad2-Cdc20 assembly, phosphorylation site mutants, live-cell imaging Science High 33384372
2022 Mps1-phosphorylated Mad1 creates a phosphorylation-specific interaction with Cdc20, and together with Mps1-phosphorylation-dependent Bub1-Mad1 association, generates a tripartite Bub1-Cdc20-Mad1CTD assembly. This positions the Cdc20 MIM (Mad2-interacting motif) near open-Mad2, catalyzing formation of C-Mad2:Cdc20. Structural analysis (NMR/biochemical), phosphorylation assays, crosslinking mass spectrometry, mutagenesis Nature communications High 36289199
2021 CDC20 targets GSDME for ubiquitination and degradation in a degron-dependent manner; CDC20 knockdown increases GSDME abundance, switching cell death from apoptosis to pyroptosis. CDC20 inhibition enhances anti-tumor immunity in syngeneic prostate cancer models by promoting CD8+ T cell infiltration in a GSDME-dependent manner. Immunoprecipitation, ubiquitination assay, cycloheximide assay, syngeneic murine tumor models, flow cytometry Experimental hematology & oncology High 37528490
2021 CDC20 interacts with and promotes ubiquitination and APC11-dependent degradation of NF-κB p65 via its WD40 domain binding the p65 DNA-binding domain. Cdc20 conditional knockout mice show decreased bone formation; p65 knockdown rescues bone loss in Cdc20 CKO mice, establishing a cell-cycle-independent CDC20-APC11-p65 axis in osteogenesis. Co-immunoprecipitation, ubiquitination assay, Cdc20 conditional KO mice, p65 knockdown rescue, WD40 domain binding assay EMBO reports High 34382737
2020 CDC20 binds to the D-box motif in PHD3 to promote its polyubiquitination and degradation, stabilizing HIF-1α and promoting VEGF secretion in hepatocellular carcinoma cells. PHD3 depletion in CDC20-knockdown cells rescues HIF-1α levels, establishing the CDC20-PHD3-HIF-1α axis. Co-immunoprecipitation, ubiquitination assay, genetic epistasis (PHD3 knockdown rescue), D-box mutant Cancer letters Medium 33039559
2018 CDC20 ubiquitinates MEIS1 and p21, targeting them for degradation; PPM1K phosphatase promotes BCAA catabolism which reduces CDC20-mediated ubiquitination of MEIS1 and p21, maintaining HSC quiescence. Ubiquitination assay, co-immunoprecipitation, Ppm1k knockout mice, hematopoietic reconstitution Cell reports Medium 29719258
2012 RAP80 is ubiquitinated and degraded by APC/C(Cdc20) during mitosis in a destruction-box-dependent manner; Cdc20 knockdown blocks RAP80 degradation during mitosis. Overexpression of a RAP80 D-box deletion mutant attenuates mitotic progression. siRNA knockdown, ubiquitination assay, D-box mutant analysis, cell cycle assay Molecular cancer research Medium 22426463
2017 In fission yeast, APC/C-Cdc20 removes PP2A from centromeres by targeting shugoshin Sgo1 and kinase Mps1 for degradation during meiosis II, allowing Rec8 deprotection and cohesin cleavage at centromeres for sister chromatid disjunction. Genetic epistasis in fission yeast, protein stability assays, centromere cohesin analysis Cell cycle Medium 28514186

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1998 The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation. Genes & development 507 9637688
1998 Budding yeast Cdc20: a target of the spindle checkpoint. Science (New York, N.Y.) 467 9461437
1998 Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1. Molecular cell 427 9734353
2000 Mitotic regulation of the APC activator proteins CDC20 and CDH1. Molecular biology of the cell 363 10793135
2007 Cdc20: a WD40 activator for a cell cycle degradation machine. Molecular cell 299 17612486
2002 Regulation of APC-Cdc20 by the spindle checkpoint. Current opinion in cell biology 289 12473343
2004 The tumour suppressor RASSF1A regulates mitosis by inhibiting the APC-Cdc20 complex. Nature cell biology 270 14743218
2001 Substrate recognition by the Cdc20 and Cdh1 components of the anaphase-promoting complex. Genes & development 203 11562349
1998 The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis. Current biology : CB 197 9651679
2000 Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20. Nature structural biology 167 10700282
2009 A centrosomal Cdc20-APC pathway controls dendrite morphogenesis in postmitotic neurons. Cell 164 19167333
2010 Mps1 directs the assembly of Cdc20 inhibitory complexes during interphase and mitosis to control M phase timing and spindle checkpoint signaling. The Journal of cell biology 160 20624902
2001 Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores. The EMBO journal 159 11726501
2002 The roles of Fzy/Cdc20 and Fzr/Cdh1 in regulating the destruction of cyclin B in space and time. The Journal of cell biology 139 12082076
2001 Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint. The EMBO journal 136 11707408
2014 Increased CDC20 expression is associated with development and progression of hepatocellular carcinoma. International journal of oncology 132 25069850
2003 Phosphorylation of Cdc20 is required for its inhibition by the spindle checkpoint. Nature cell biology 132 12855955
2017 Cdc20: At the Crossroads between Chromosome Segregation and Mitotic Exit. Trends in biochemical sciences 124 28202332
2014 APC(Cdc20) suppresses apoptosis through targeting Bim for ubiquitination and destruction. Developmental cell 124 24871945
1999 Phosphorylation of the cyclosome is required for its stimulation by Fizzy/cdc20. Biochemical and biophysical research communications 123 10381365
1997 Cell cycle-regulated expression, phosphorylation, and degradation of p55Cdc. A mammalian homolog of CDC20/Fizzy/slp1. The Journal of biological chemistry 120 9353311
2012 APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex. Nature structural & molecular biology 119 23007861
2007 Mad3 KEN boxes mediate both Cdc20 and Mad3 turnover, and are critical for the spindle checkpoint. PloS one 115 17406666
1998 Mad2 transiently associates with an APC/p55Cdc complex during mitosis. Proceedings of the National Academy of Sciences of the United States of America 114 9736712
2012 Increased CDC20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression. Journal of hematology & oncology 107 22475564
2003 Cks1-dependent proteasome recruitment and activation of CDC20 transcription in budding yeast. Nature 105 12827207
2001 The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner. Current biology : CB 105 11553328
1999 Identification of frequent impairment of the mitotic checkpoint and molecular analysis of the mitotic checkpoint genes, hsMAD2 and p55CDC, in human lung cancers. Oncogene 105 10439037
2009 A Cdc20-APC ubiquitin signaling pathway regulates presynaptic differentiation. Science (New York, N.Y.) 103 19900895
2011 How APC/C-Cdc20 changes its substrate specificity in mitosis. Nature cell biology 97 21336306
2007 Loss of Cdc20 causes a securin-dependent metaphase arrest in two-cell mouse embryos. Molecular and cellular biology 97 17325031
2016 The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation. Nature communications 90 26912231
2012 Dephosphorylation of Cdc20 is required for its C-box-dependent activation of the APC/C. The EMBO journal 88 22713866
2012 Structural analysis of human Cdc20 supports multisite degron recognition by APC/C. Proceedings of the National Academy of Sciences of the United States of America 85 23091007
2010 Cdc20 is critical for meiosis I and fertility of female mice. PLoS genetics 85 20941357
2021 CDC20 regulates the cell proliferation and radiosensitivity of P53 mutant HCC cells through the Bcl-2/Bax pathway. International journal of biological sciences 82 34512169
2012 Cell cycle control of Wnt/β-catenin signalling by conductin/axin2 through CDC20. EMBO reports 82 22322943
2015 Parkin Regulates Mitosis and Genomic Stability through Cdc20/Cdh1. Molecular cell 78 26387737
2019 Cell division cycle 20 (CDC20) drives prostate cancer progression via stabilization of β-catenin in cancer stem-like cells. EBioMedicine 73 30904606
2014 The master cell cycle regulator APC-Cdc20 regulates ciliary length and disassembly of the primary cilium. eLife 70 25139956
2012 Mad2 and the APC/C compete for the same site on Cdc20 to ensure proper chromosome segregation. The Journal of cell biology 67 23007648
2011 Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in Arabidopsis thaliana. PloS one 64 21687678
2005 Low concentrations of taxol cause mitotic delay followed by premature dissociation of p55CDC from Mad2 and BubR1 and abrogation of the spindle checkpoint, leading to aneuploidy. Cell cycle (Georgetown, Tex.) 62 16138009
2020 APCCDC20-mediated degradation of PHD3 stabilizes HIF-1a and promotes tumorigenesis in hepatocellular carcinoma. Cancer letters 60 33039559
2018 PPM1K Regulates Hematopoiesis and Leukemogenesis through CDC20-Mediated Ubiquitination of MEIS1 and p21. Cell reports 60 29719258
1999 Cdc20 associates with the kinase aurora2/Aik. Proceedings of the National Academy of Sciences of the United States of America 59 10377410
2019 CDC20 associated with cancer metastasis and novel mushroom‑derived CDC20 inhibitors with antimetastatic activity. International journal of oncology 57 31081056
2000 p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase. Oncogene 55 11030144
2017 Kinetochores accelerate or delay APC/C activation by directing Cdc20 to opposing fates. Genes & development 54 28698300
2016 Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry. Nature communications 51 26960431
2018 Cdc20 inhibitor apcin inhibits the growth and invasion of osteosarcoma cells. Oncology reports 49 29901174
2022 Targeting Cdc20 for cancer therapy. Biochimica et biophysica acta. Reviews on cancer 47 36243246
2021 A tripartite mechanism catalyzes Mad2-Cdc20 assembly at unattached kinetochores. Science (New York, N.Y.) 47 33384372
2010 Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F. Journal of cell science 47 20053638
2010 APC/C(Cdc20) targets E2F1 for degradation in prometaphase. Cell cycle (Georgetown, Tex.) 47 20948288
2016 Prostate cancer-associated mutation in SPOP impairs its ability to target Cdc20 for poly-ubiquitination and degradation. Cancer letters 46 27780719
2009 DNA damage induced p53 downregulates Cdc20 by direct binding to its promoter causing chromatin remodeling. Nucleic acids research 46 19273532
2002 Cyclin destruction in mitosis: a crucial task of Cdc20. FEBS letters 45 12459453
2021 Inhibition of Cdc20 suppresses the metastasis in triple negative breast cancer (TNBC). Breast cancer (Tokyo, Japan) 39 33813687
2011 Ubiquitination of Cdc20 by the APC occurs through an intramolecular mechanism. Current biology : CB 39 22079111
2019 Elevated signature of a gene module coexpressed with CDC20 marks genomic instability in glioma. Proceedings of the National Academy of Sciences of the United States of America 38 30877245
2017 Inhibition of Cell Survival by Curcumin Is Associated with Downregulation of Cell Division Cycle 20 (Cdc20) in Pancreatic Cancer Cells. Nutrients 37 28165402
2010 Defining the molecular basis of BubR1 kinetochore interactions and APC/C-CDC20 inhibition. The Journal of biological chemistry 37 20220147
2017 Cell Cycle Control by Nuclear Sequestration of CDC20 and CDH1 mRNA in Plant Stem Cells. Molecular cell 36 29225038
2016 Rottlerin inhibits cell growth and invasion via down-regulation of Cdc20 in glioma cells. Oncotarget 35 27626499
2023 Inhibition of CDC20 potentiates anti-tumor immunity through facilitating GSDME-mediated pyroptosis in prostate cancer. Experimental hematology & oncology 34 37528490
2008 Targeting of CDC20 via small interfering RNA causes enhancement of the cytotoxicity of chemoradiation. Anticancer research 32 18630511
2019 CDC20 contributes to the development of human cutaneous squamous cell carcinoma through the Wnt/β‑catenin signaling pathway. International journal of oncology 31 30816486
2010 Cyclin A and Nek2A: APC/C-Cdc20 substrates invisible to the mitotic spindle checkpoint. Biochemical Society transactions 30 20074038
2021 CDC20 and PTTG1 are Important Biomarkers and Potential Therapeutic Targets for Metastatic Prostate Cancer. Advances in therapy 26 33881746
2019 Hyperphosphorylation of CDH1 in Glioblastoma Cancer Stem Cells Attenuates APC/CCDH1 Activity and Pharmacologic Inhibition of APC/CCDH1/CDC20 Compromises Viability. Molecular cancer research : MCR 26 31036696
2012 Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex. Proceedings of the National Academy of Sciences of the United States of America 26 23267073
2004 Speriolin is a novel spermatogenic cell-specific centrosomal protein associated with the seventh WD motif of Cdc20. The Journal of biological chemistry 26 15280373
2022 Impaired Cdc20 signaling promotes senescence in normal cells and apoptosis in non-small cell lung cancer cells. The Journal of biological chemistry 25 35988650
2008 Functions of FZR1 and CDC20, activators of the anaphase-promoting complex, during meiotic maturation of swine oocytes. Biology of reproduction 25 18753608
2023 CDC20: a novel therapeutic target in cancer. American journal of translational research 24 36915766
2022 Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly. Nature communications 23 36289199
2017 APC/C-Cdc20 mediates deprotection of centromeric cohesin at meiosis II in yeast. Cell cycle (Georgetown, Tex.) 23 28514186
2012 Degradation of human RAP80 is cell cycle regulated by Cdc20 and Cdh1 ubiquitin ligases. Molecular cancer research : MCR 23 22426463
2020 PP1 promotes cyclin B destruction and the metaphase-anaphase transition by dephosphorylating CDC20. Molecular biology of the cell 22 32755477
2017 Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex. Current biology : CB 22 28366743
2015 Bub3 promotes Cdc20-dependent activation of the APC/C in S. cerevisiae. The Journal of cell biology 22 25987604
2010 Recruitment of Cdc20 to the kinetochore requires BubR1 but not Mad2 in Drosophila melanogaster. Molecular and cellular biology 22 20421417
2022 The A20/TNFAIP3-CDC20-CASP1 Axis Promotes Inflammation-mediated Metastatic Disease in Triple-negative Breast Cancer. Anticancer research 21 35093867
2009 Cell-cycle-dependent PC-PLC regulation by APC/C(Cdc20)-mediated ubiquitin-proteasome pathway. Journal of cellular biochemistry 21 19347873
2021 Novel Mutations in CDC20 Are Associated with Female Infertility Due to Oocyte Maturation Abnormality and Early Embryonic Arrest. Reproductive sciences (Thousand Oaks, Calif.) 19 33683667
2021 CDC20 promotes bone formation via APC/C dependent ubiquitination and degradation of p65. EMBO reports 19 34382737
2015 BUB1B promotes multiple myeloma cell proliferation through CDC20/CCNB axis. Medical oncology (Northwood, London, England) 19 25698537
2013 CDC20 downregulation impairs spindle morphology and causes reduced first polar body emission during bovine oocyte maturation. Theriogenology 19 24360405
2005 Bub1 and the multilayered inhibition of Cdc20-APC/C in mitosis. Trends in cell biology 19 15866025
2020 Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells. International journal of molecular sciences 18 32932732
2020 MDM2-P53 Signaling Pathway-Mediated Upregulation of CDC20 Promotes Progression of Human Diffuse Large B-Cell Lymphoma. OncoTargets and therapy 18 33116627
2001 Transcriptional upregulation and activation of p55Cdc via p34(cdc2) in Taxol-induced apoptosis. Oncogene 18 11420663
2019 PP2A-B56 binds to Apc1 and promotes Cdc20 association with the APC/C ubiquitin ligase in mitosis. EMBO reports 17 31825153
2017 R383C mutation of human CDC20 results in idiopathic non-obstructive azoospermia. Oncotarget 17 29245942
2016 O-GlcNAcylation Antagonizes Phosphorylation of CDH1 (CDC20 Homologue 1). The Journal of biological chemistry 17 27080259
1998 DNA polymerase epsilon encoded by cdc20+ is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe. Genes to cells : devoted to molecular & cellular mechanisms 17 9605404
2023 The potential role of CDC20 in tumorigenesis, cancer progression and therapy: A narrative review. Medicine 16 37682144
2022 Chromobox 4 (CBX4) promotes tumor progression and stemness via activating CDC20 in gastric cancer. Journal of gastrointestinal oncology 16 35837165
2019 Diosgenin exerts its tumor suppressive function via inhibition of Cdc20 in osteosarcoma cells. Cell cycle (Georgetown, Tex.) 16 30640578