Affinage

CDC27

Cell division cycle protein 27 homolog · UniProt P30260

Length
824 aa
Mass
91.9 kDa
Annotated
2026-04-28
68 papers in source corpus 20 papers cited in narrative 20 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CDC27 (APC3) is a core TPR-repeat subunit of the anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that drives mitotic progression by targeting cyclins, securin, and other substrates for proteasomal destruction. CDC27 homodimerizes through a conserved N-terminal TPR superhelix essential for function, and its C-terminal IR tail-binding cleft recruits the coactivators CDC20 and CDH1, while also mediating incorporation of APC16 and APC7 (PMID:7736580, PMID:20206185, PMID:25490258). Cdk1 phosphorylation of CDC27 is required for its chromosomal localization during mitosis and for APC/C-dependent degradation of cyclin A and cyclin B, whereas casein kinase II phosphorylation at Ser154 activates APC/C toward the TGF-β effector SnoN; phosphatases PP5 and PP2A counteract these modifications (PMID:17519285, PMID:12953067, PMID:21209074, PMID:9405394). CDC27 protein stability is maintained by O-GlcNAcylation that prevents autophagy-lysosome-mediated degradation, and its translation is post-transcriptionally repressed by HNRNP E1, whose loss causes constitutive APC/C activation, premature CDH1 destruction, and aneuploidy (PMID:39984622, PMID:27102006).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1995 High

    Establishing that CDC27 is a catalytically essential subunit of the APC/C resolved the identity of the E3 ligase responsible for mitotic cyclin B ubiquitination.

    Evidence Immunodepletion and immunopurification from Xenopus egg extracts reconstituted cyclin B ubiquitination in vitro

    PMID:7736580

    Open questions at the time
    • Specific contribution of CDC27 versus other APC/C subunits to substrate recognition was unknown
    • No structural information on CDC27 at this stage
  2. 1996 Medium

    Demonstrating that CDC27 mutants cause DNA overreplication in yeast revealed an APC/C role in restricting S-phase re-initiation, extending its function beyond anaphase onset.

    Evidence Temperature-sensitive cdc27 mutants in S. cerevisiae showed up to 8C DNA content by flow cytometry

    PMID:8620535

    Open questions at the time
    • The specific APC/C substrate(s) responsible for licensing control were not identified
    • No direct measurement of APC/C activity in this context
  3. 1997 Medium

    Identification of PP5 as a CDC27-interacting phosphatase provided the first evidence for regulated dephosphorylation of APC/C subunits.

    Evidence Yeast two-hybrid, in vitro pulldown, and colocalization of PP5 with CDC27 at the mitotic spindle

    PMID:9405394

    Open questions at the time
    • No direct demonstration that PP5 dephosphorylates CDC27 or alters APC/C activity
    • Functional consequence of PP5-CDC27 interaction on cell cycle was not tested
  4. 1998 Medium

    Showing that CDC27 is required for destruction-box-independent proteolysis of Cdc20 established that the APC/C coactivator is itself an APC/C substrate requiring CDC27.

    Evidence Genetic analysis of Cdc20 stability in S. cerevisiae cdc27 mutants

    PMID:9651679

    Open questions at the time
    • Mechanism of destruction-box-independent recognition was not defined
    • Whether this reflects a direct CDC27-Cdc20 interaction or an indirect requirement was unclear
  5. 2002 High

    Demonstrating that dephosphorylated CDC27 localizes to kinetochores and that anti-CDC27 antibody injection causes metaphase arrest placed CDC27 functionally downstream of the spindle assembly checkpoint.

    Evidence Immunofluorescence, chromosome fractionation, and antibody microinjection in mammalian cells

    PMID:12429948

    Open questions at the time
    • Identity of kinase(s) responsible for CDC27 phosphorylation at chromosomes was inferred but not directly shown
    • How phosphorylation state controls chromosome binding was not structurally resolved
  6. 2003 High

    Genetic epistasis in Drosophila established that Cdk1 phosphorylation of Cdc27 is required for cyclin A/B degradation and that PP2A acts as a downstream antagonist of APC/C-Cdc27.

    Evidence Drosophila cdc27 (mákos) mutant neuroblasts arrest in metaphase with elevated cyclins; PP2A (twins/aar) mutations suppress the arrest

    PMID:12953067

    Open questions at the time
    • Direct phosphorylation sites on Drosophila Cdc27 were not mapped in this study
    • Whether PP2A acts directly on CDC27 or on APC/C substrates was ambiguous
  7. 2004 High

    Identification of Swm1/Apc13 as a factor required for stable CDC27 incorporation into APC/C revealed a hierarchical assembly dependency among APC/C subunits.

    Evidence Mass spectrometry, yeast genetics, and in vitro ubiquitination assays across species

    PMID:15060174

    Open questions at the time
    • Structural basis of the Swm1-CDC27 stabilization was not determined
  8. 2007 High

    Mapping of two Cdk1 phosphorylation sites on Drosophila Cdc27 and showing that their mutation abolishes chromosomal localization and APC/C function provided the first mechanistic link between Cdk1 phosphorylation, CDC27 localization, and substrate degradation in vivo.

    Evidence GFP::Cdc27 phosphosite mutagenesis with live imaging and in vivo rescue in Drosophila embryos

    PMID:17519285

    Open questions at the time
    • Whether these sites are conserved and functionally equivalent in mammalian CDC27 was not tested
    • Binding partner that recruits phospho-CDC27 to chromosomes was unknown
  9. 2010 High

    Crystal structures of CDC27 homologs revealed that CDC27 homodimerizes via an N-terminal TPR superhelix and that dimerization is essential for APC/C function, providing the first atomic-resolution view of APC/C subunit architecture.

    Evidence X-ray crystallography of E. cuniculi CDC27 and S. pombe Cut9-Hcn1 complex, validated by mutagenesis and yeast complementation

    PMID:20206185 PMID:20924356

    Open questions at the time
    • Full-length human CDC27 structure was not yet available
    • How the dimer integrates into the full APC/C architecture was not resolved
  10. 2010 High

    Demonstrating that CDC27/APC/C-CDH1 ubiquitinates cyclin D1 downstream of C/EBPδ transcriptional induction expanded the APC/C substrate repertoire to G1 regulators.

    Evidence siRNA knockdown, in vitro ubiquitination assay, and C/EBPδ knockout MEFs

    PMID:20439707

    Open questions at the time
    • Whether cyclin D1 is a direct CDC27-dependent substrate or requires additional specificity factors was not fully resolved
  11. 2011 High

    Crystallographic determination of the MCPH1 BRCT–phospho-CDC27 interaction identified a specific reader of CDC27 phosphorylation, linking microcephalin to APC/C regulation.

    Evidence X-ray crystallography of MCPH1 C-BRCT with phospho-CDC27 peptide, mutagenesis, co-immunoprecipitation

    PMID:22139841

    Open questions at the time
    • Functional consequence of MCPH1-CDC27 interaction on APC/C activity or cell cycle progression was not defined
  12. 2011 High

    Identification of CKII-mediated Ser154 phosphorylation of CDC27 as the signal activating APC/C for SnoN degradation connected CDC27 to the TGF-β signaling pathway.

    Evidence CKII knockdown, CDC27-S154A mutagenesis, SnoN degradation and growth inhibition assays

    PMID:21209074

    Open questions at the time
    • Whether CKII phosphorylation of CDC27 affects other APC/C substrates was not examined
    • Structural basis of how Ser154 phosphorylation activates APC/C was not resolved
  13. 2014 High

    The crystal structure of human APC3 (CDC27) in complex with APC16 revealed the asymmetric subunit interface and the C-terminal IR tail-binding cleft that recruits coactivators CDC20 and CDH1, defining CDC27 as the central coactivator docking platform.

    Evidence X-ray crystallography of human APC3Δloop alone and with APC16, domain mapping, in vitro ubiquitination assays

    PMID:25490258

    Open questions at the time
    • Whether the phosphorylation loop has a regulatory role in vivo despite being dispensable in vitro was unresolved
  14. 2016 High

    Discovering that HNRNP E1 represses CDC27 mRNA translation revealed a post-transcriptional control mechanism; its loss deregulates APC/C activity, prematurely destroys CDH1, and causes aneuploidy.

    Evidence HNRNP E1 knockdown with polysome profiling, flow cytometry for ploidy, APC/C activity assays in mammary epithelial cells

    PMID:27102006

    Open questions at the time
    • The RNA element in CDC27 mRNA recognized by HNRNP E1 was not mapped
    • Whether this regulatory axis operates in non-epithelial tissues was not tested
  15. 2023 Medium

    Showing that Mad2B/Rev7 forms a constitutive complex with CDC27 and that cisplatin-induced DNA damage recruits Cdc20 to activate APC/C linked the APC/C-CDC27 axis to the DNA damage response.

    Evidence Co-immunoprecipitation and in vitro ubiquitination assay following cisplatin treatment

    PMID:37641805

    Open questions at the time
    • The physiological substrate(s) of Mad2B-activated APC/C-Cdc20 after DNA damage were not identified
    • Whether Mad2B directly contacts CDC27 or binds via another subunit was not resolved
  16. 2025 Medium

    Demonstrating that OGT-mediated O-GlcNAcylation stabilizes CDC27 by blocking autophagy-lysosome-dependent degradation revealed a nutrient-sensing input controlling APC/C subunit abundance.

    Evidence OGT knockdown, OSMI-1 pharmacological inhibition, mass spectrometry-confirmed O-GlcNAcylation, autophagy/lysosome inhibitor rescue in multiple myeloma cells

    PMID:39984622

    Open questions at the time
    • Specific O-GlcNAcylation sites on CDC27 were not mapped
    • Whether O-GlcNAcylation affects CDC27 activity independently of stability was not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unknown how CDC27 phosphorylation, O-GlcNAcylation, and coactivator binding are coordinately regulated to tune APC/C activity across different cell cycle phases and signaling contexts, and no high-resolution structure of full-length human CDC27 within the complete APC/C complex has captured these regulatory modifications simultaneously.
  • No integrated structural model incorporating phosphorylation, O-GlcNAcylation, and coactivator binding on CDC27
  • Relative contributions of Cdk1 vs. CKII phosphorylation to APC/C activation in different contexts are not delineated
  • Mechanism by which phosphorylated CDC27 is recruited to chromosomes is structurally unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 4 GO:0005198 structural molecule activity 3
Localization
GO:0005634 nucleus 2 GO:0005694 chromosome 2
Pathway
R-HSA-1640170 Cell Cycle 5 R-HSA-392499 Metabolism of proteins 4 R-HSA-162582 Signal Transduction 1
Partners
APC16APC7CDC16CDC20CDH1MAD2L2MCPH1PP5
Complex memberships
APC/C

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 CDC27 (APC3) is a core subunit of the 20S anaphase-promoting complex (APC/C), a ubiquitin-protein ligase that catalyzes mitosis-specific ubiquitin conjugation to cyclin B, targeting it for destruction at anaphase onset. CDC27 antibody depletion abolishes APC activity, and immunopurified CDC27 complexes can reconstitute cyclin B ubiquitination with UBC4 and E1. Biochemical fractionation of Xenopus egg extracts, immunodepletion with anti-CDC27 antibodies, immunopurification and in vitro ubiquitination reconstitution assay Cell High 7736580
1997 The serine/threonine phosphatase PP5 physically interacts with CDC27 (and CDC16) through its N-terminal tetratricopeptide repeat (TPR) domain; the binding site on CDC27 maps to its C-terminal block of TPR repeats. PP5 colocalizes with CDC27 at the mitotic spindle apparatus, suggesting PP5 regulates APC/C activity via dephosphorylation. Yeast two-hybrid analysis, in vitro binding/pulldown assays, indirect immunofluorescence localization The Journal of biological chemistry Medium 9405394
1998 In budding yeast, CDC27 and CDC23 (APC subunits) are required for destruction-box-independent proteolysis of Cdc20 during S phase and early mitosis, establishing that Cdc20 is itself a substrate of the APC dependent on CDC27. Genetic analysis in S. cerevisiae; cell-cycle-regulated protein stability assays in cdc27 and cdc23 mutants Current biology Medium 9651679
2002 Dephosphorylated CDC27/APC3 localizes to kinetochores and chromosome arms during mitosis in mammalian cells, independent of microtubules. Only the dephosphorylated form associates with isolated chromosomes and is phosphorylated by chromosome-associated kinases in vitro. Microinjection of anti-Cdc27 antibody causes metaphase arrest and blocks the premature anaphase otherwise induced by anti-Mad2 antibody, placing APC/C CDC27 downstream of the spindle checkpoint. Immunofluorescence microscopy, chromosome isolation and fractionation, in vitro kinase assay, antibody microinjection into mammalian cells Cell cycle High 12429948
2004 The small APC/C subunit Swm1/Apc13 promotes the stable association of CDC27 (and CDC16) within the APC/C complex; loss of Swm1 reduces APC/C ubiquitin ligase activity in vitro and delays APC/C-dependent cell cycle events in vivo. Yeast genetics, mass spectrometry interactome, in vitro ubiquitination assay, cross-species complementation Molecular and cellular biology High 15060174
2010 Crystal structure of the N-terminal domain of Encephalitozoon cuniculi CDC27 reveals a homo-dimeric TPR superhelix. Mutation of the dimer interface destabilizes the protein, disrupts dimerization in solution, and abolishes the ability of CDC27 to complement S. cerevisiae cdc27 deletion in vivo, demonstrating the functional importance of CDC27 dimerization. X-ray crystallography, analytical ultracentrifugation/gel filtration, site-directed mutagenesis, yeast complementation assay Journal of molecular biology High 20206185
2010 CDC27 (APC3) and CDC16 (APC6/Cut9) both form homo-dimers through a conserved N-terminal TPR interface, as revealed by the crystal structure of S. pombe Cut9 in complex with Hcn1 (Cdc26). The acetylated N-terminal Met of Hcn1 is buried within the Cut9 TPR chamber, protecting Hcn1 from Doa10-mediated ubiquitin-dependent degradation. X-ray crystallography of S. pombe Cut9–Hcn1 complex The EMBO journal High 20924356
2010 C/EBPδ transcriptionally induces CDC27 (APC3) expression, which then directs polyubiquitination and proteasomal degradation of cyclin D1 via APC/C-CDH1. Silencing of CDC27 or CDH1 in breast epithelial cells increases cyclin D1 protein levels; in C/EBPδ knockout MEFs, CDC27 and its activity toward cyclin D1 are reduced. siRNA/shRNA knockdown, overexpression, immunoprecipitation, in vitro ubiquitination assay, C/EBPδ knockout MEFs Proceedings of the National Academy of Sciences of the United States of America High 20439707
2011 The C-terminal tandem BRCT domains of microcephalin (MCPH1) bind phosphorylated CDC27 in a phosphorylation-dependent manner. The crystal structure of MCPH1 C-BRCTs in complex with a phospho-CDC27 peptide was determined; point mutations at the binding interface disrupted the interaction in vitro and in vivo. Co-immunoprecipitation, in vitro binding assay, X-ray crystallography, site-directed mutagenesis The Journal of biological chemistry High 22139841
2011 Casein kinase II (CKII) phosphorylates CDC27 at Ser154 in response to TGF-β signaling, which activates APC/C to ubiquitinate and degrade SnoN. Knockdown of CKII abolishes TGF-β-induced CDC27 phosphorylation and SnoN degradation; a phosphorylation-resistant CDC27-S154A mutant attenuates TGF-β-induced growth inhibition. shRNA knockdown of CKII, phosphorylation-resistant mutagenesis (S154A), SnoN degradation assays, growth inhibition assays The Journal of biological chemistry High 21209074
2012 Curcumin directly binds to and crosslinks CDC27/APC3, inhibiting APC/C ubiquitin ligase activity and inducing apoptosis preferentially in cells containing phosphorylated CDC27 (i.e., highly proliferating/cancer cells). Curcumin pulldown assays, in vitro APC/C ubiquitination activity assay, cell cycle profiling BMC cancer Medium 22280307
2014 Crystal structure of human APC3 (CDC27) Δloop domain alone and in complex with the C-terminal domain of APC16 reveals asymmetric binding of one APC16 molecule to the symmetric APC3 dimer. APC3's C-terminal IR tail-binding cleft recruits APC/C coactivators CDC20 and CDH1; APC3 also mediates recruitment of APC7. The APC3 phosphorylation loop is not required for APC/C-catalyzed ubiquitination in vitro. X-ray crystallography, domain mapping/biochemistry, in vitro APC/C ubiquitination assay Journal of molecular biology High 25490258
2016 The RNA-binding protein HNRNP E1/PCBP1 post-transcriptionally silences translation of the CDC27 mRNA in normal mammary epithelial cells. Loss of HNRNP E1 causes constitutive CDC27 expression, leading to premature activation of APC/C-CDC20, aberrant degradation of CDH1/FZR1, mitotic aberrations, and aneuploidy. shRNA knockdown of HNRNP E1, polysome profiling/translational assays, flow cytometry for aneuploidy, APC/C activity assays Molecular cancer research High 27102006
2003 In Drosophila, Cdc27 (mákos gene product) is required for APC/C-mediated degradation of cyclin A and cyclin B during mitosis; mks mutant neuroblasts arrest in a metaphase-like state with elevated levels of both cyclins. Cdk1 phosphorylation of Cdc27 is required for this function. Mutations in twins/aar (PP2A regulatory subunit) suppress the mks metaphase arrest, defining PP2A as a negative regulator acting downstream of APC/C-Cdc27. Drosophila genetics, immunostaining for cyclins, epistasis analysis with spindle checkpoint (bub1) and PP2A (twins/aar) mutants Journal of cell science High 12953067
2007 Cdk1 phosphorylation at two conserved sites (Pro304 and Pro456 motifs) in Drosophila Cdc27 is required for its chromosomal localization during mitosis and for APC/C function in vivo. Double phosphorylation-site mutation (P304A, P456A) prevents chromosomal localization of GFP::Cdc27 and fails to rescue cdc27 mutant phenotypes, with accumulation of cyclin A and cyclin B. GFP::Cdc27 live imaging in syncytial Drosophila embryos, site-directed mutagenesis, in vivo complementation assay, immunoblotting for cyclins Journal of cell science High 17519285
2016 CDC27 downregulation in colorectal cancer cells causes G1/S arrest via accumulation of p21Cip1/Waf1, mediated through modulation of ID1 protein expression. Overexpression of CDC27 promotes G1/S transition by attenuating p21 through the ID1 pathway. shRNA knockdown and overexpression of CDC27, cell cycle flow cytometry, immunoblotting for p21 and ID1, rescue assays Cell death & disease Medium 26821069
2025 O-GlcNAcylation of CDC27 by OGT maintains CDC27 protein stability by blocking its degradation via the autophagy-lysosome pathway (ALP). Inhibition of O-GlcNAcylation (OSMI-1 or OGT knockdown) reduces CDC27 protein levels in multiple myeloma cells; mass spectrometry confirmed O-GlcNAcylated CDC27 as a direct target of OSMI-1. siRNA knockdown of OGT, pharmacological inhibition (OSMI-1), pharmacological accumulation (Thiamet G), mass spectrometry, immunoprecipitation, autophagy/lysosome inhibitor assays Acta pharmacologica Sinica Medium 39984622
2023 Mad2B (Mad2L2/Rev7) forms a complex with CDC27 in undamaged cells together with Polζ subunits Rev3 and Rev1; following cisplatin-induced DNA damage, Cdc20 is additionally recruited to this complex. In vitro ubiquitination assay confirmed Mad2B-dependent activation of APC/C-Cdc20 following cisplatin treatment. Co-immunoprecipitation, in vitro ubiquitination assay, immunofluorescence localization upon DNA damage The Korean journal of physiology & pharmacology Medium 37641805
1996 In S. cerevisiae, CDC16 and CDC27 restrict DNA replication to once per cell cycle; temperature-sensitive mutants of CDC16 and CDC27 cause DNA overreplication (up to 8C DNA content) within a single cell cycle without requiring passage through mitosis, suggesting they target an S phase initiator for proteolysis. Yeast genetics, flow cytometry for DNA content, cell-cycle analysis Cell Medium 8620535
2016 Elmo1 interacts with CDC27 through Elmo1's C-terminal region (essential for Dock1 association). Dock1 binding to Elmo1 abrogates Elmo1–CDC27 interaction, but CDC27 does not affect Elmo1–Dock1–Rac-mediated phagocytosis. Yeast two-hybrid, co-immunoprecipitation in mammalian cells, pulldown assays, phagocytosis assay Biochemical and biophysical research communications Low 26882976

Source papers

Stage 0 corpus · 68 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B. Cell 843 7736580
1990 Snap helix with knob and hole: essential repeats in S. pombe nuclear protein nuc2+. Cell 271 2297790
1999 Cloning genes encoding MHC class II-restricted antigens: mutated CDC27 as a tumor antigen. Science (New York, N.Y.) 242 10334988
1988 A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase. The Journal of cell biology 206 3283148
1998 The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis. Current biology : CB 197 9651679
2002 The Arabidopsis HOBBIT gene encodes a CDC27 homolog that links the plant cell cycle to progression of cell differentiation. Genes & development 126 12368267
1996 The fission yeast Cdc1 protein, a homologue of the small subunit of DNA polymerase delta, binds to Pol3 and Cdc27. The EMBO journal 88 8887553
1996 The yeast CDC16 and CDC27 genes restrict DNA replication to once per cell cycle. Cell 80 8620535
2007 Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C). The Plant journal : for cell and molecular biology 68 17944809
1997 The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex. The Journal of biological chemistry 67 9405394
2010 C/EBP{delta} targets cyclin D1 for proteasome-mediated degradation via induction of CDC27/APC3 expression. Proceedings of the National Academy of Sciences of the United States of America 65 20439707
2010 The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat superhelix with a homo-dimer interface similar to Cdc27. The EMBO journal 63 20924356
2015 MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27. Medical science monitor : international medical journal of experimental and clinical research 62 25943633
2004 Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27. Molecular and cellular biology 62 15060174
1993 Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base. Proceedings of the National Academy of Sciences of the United States of America 58 8234252
1990 In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells. The Journal of cell biology 57 2404993
1995 Fission yeast TPR-family protein nuc2 is required for G1-arrest upon nitrogen starvation and is an inhibitor of septum formation. Journal of cell science 56 7622618
2014 Staphylococcus aureus Nuc2 is a functional, surface-attached extracellular nuclease. PloS one 54 24752186
2006 Non-cell-autonomous rescue of anaphase-promoting complex function revealed by mosaic analysis of HOBBIT, an Arabidopsis CDC27 homolog. Proceedings of the National Academy of Sciences of the United States of America 54 16938844
2000 Essential interaction between the fission yeast DNA polymerase delta subunit Cdc27 and Pcn1 (PCNA) mediated through a C-terminal p21(Cip1)-like PCNA binding motif. The EMBO journal 53 10698951
2016 Downregulation of CDC27 inhibits the proliferation of colorectal cancer cells via the accumulation of p21Cip1/Waf1. Cell death & disease 45 26821069
2002 The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell cycle (Georgetown, Tex.) 45 12429948
2012 Anaphase-promoting complex/cyclosome protein Cdc27 is a target for curcumin-induced cell cycle arrest and apoptosis. BMC cancer 44 22280307
2002 The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta. The Journal of biological chemistry 42 12124382
2001 The human homologue of fission Yeast cdc27, p66, is a component of active human DNA polymerase delta. Journal of biochemistry 39 11328591
2021 The importance of CDC27 in cancer: molecular pathology and clinical aspects. Cancer cell international 34 33750395
2014 Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome. Journal of molecular biology 31 25490258
2010 Molecular structure of the N-terminal domain of the APC/C subunit Cdc27 reveals a homo-dimeric tetratricopeptide repeat architecture. Journal of molecular biology 31 20206185
2020 Hsa_circ_0044226 knockdown attenuates progression of pulmonary fibrosis by inhibiting CDC27. Aging 29 32710728
2018 CDC27 Facilitates Gastric Cancer Cell Proliferation, Invasion and Metastasis via Twist-Induced Epithelial-Mesenchymal Transition. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 29 30308498
2005 Depletion of anaphase-promoting complex or cyclosome (APC/C) subunit homolog APC1 or CDC27 of Trypanosoma brucei arrests the procyclic form in metaphase but the bloodstream form in anaphase. The Journal of biological chemistry 29 15994309
1995 Identification of a human homologue of yeast nuc2 which interacts with the retinoblastoma protein in a specific manner. Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 29 7756179
2023 LncRNA TUG1 promotes pulmonary fibrosis progression via up-regulating CDC27 and activating PI3K/Akt/mTOR pathway. Epigenetics 28 36994860
2018 Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis. Scientific reports 28 29618731
2003 Mutations in mákos, a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are suppressed by mutations in twins/aar, which encodes a regulatory subunit of PP2A. Journal of cell science 26 12953067
1996 NUC-2, a component of the phosphate-regulated signal transduction pathway in Neurospora crassa, is an ankyrin repeat protein. Molecular & general genetics : MGG 26 8917314
2017 CDC27 Induces Metastasis and Invasion in Colorectal Cancer via the Promotion of Epithelial-To-Mesenchymal Transition. Journal of Cancer 25 28900500
2011 Molecular basis for the association of microcephalin (MCPH1) protein with the cell division cycle protein 27 (Cdc27) subunit of the anaphase-promoting complex. The Journal of biological chemistry 23 22139841
1992 Molecular cloning and sequence analysis of cdc27+ required for the G2-M transition in the fission yeast Schizosaccharomyces pombe. Molecular & general genetics : MGG 21 1538696
2016 PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27. Molecular cancer research : MCR 20 27102006
2004 Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27. BMC molecular biology 19 15579205
2013 Low cdc27 and high securin expression predict short survival for breast cancer patients. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 18 23755904
2011 Phosphorylation of the anaphase-promoting complex/Cdc27 is involved in TGF-beta signaling. The Journal of biological chemistry 18 21209074
2007 Identification of genes displaying differential expression in the nuc-2 mutant strain of the mold Neurospora crassa grown under phosphate starvation. FEMS microbiology letters 17 17229059
2017 mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27. Oncotarget 16 27974673
2007 Cdk1 phosphorylation sites on Cdc27 are required for correct chromosomal localisation and APC/C function in syncytial Drosophila embryos. Journal of cell science 16 17519285
2020 Ustilago maydis secreted T2 ribonucleases, Nuc1 and Nuc2 scavenge extracellular RNA. Cellular microbiology 15 32844528
2020 CDC27 Promotes Tumor Progression and Affects PD-L1 Expression in T-Cell Lymphoblastic Lymphoma. Frontiers in oncology 13 32391258
1991 Evidence that an endo-exonuclease controlled by the NUC2 gene functions in the induction of 'petite' mutations in Saccharomyces cerevisiae. Current genetics 9 1657413
2000 Hypomorphic bimA(APC3) alleles cause errors in chromosome metabolism that activate the DNA damage checkpoint blocking cytokinesis in Aspergillus nidulans. Genetics 8 10628978
2022 A Novel Gene CDC27 Causes SLE and Is Associated With the Disease Activity. Frontiers in immunology 7 35418986
2020 Comprehensive Analysis of CDC27 Related to Peritoneal Metastasis by Whole Exome Sequencing in Gastric Cancer. OncoTargets and therapy 7 32368092
2022 Circ_0000775 promotes the migration, invasion and EMT of hepatic carcinoma cells by recruiting IGF2BP2 to stabilize CDC27. Pathology, research and practice 6 35561648
2015 Association between polymorphisms in cdc27 and breast cancer in a Chinese population. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 6 25680405
2024 Functional and Genetic Analyses Unveil the Implication of CDC27 in Hemifacial Microsomia. International journal of molecular sciences 5 38731925
2024 Implications of ITCH-mediated ubiquitination of SIX1 on CDC27-cyclinB1 signaling in nasopharyngeal carcinoma. Scientific reports 4 39406717
2018 Author Correction: Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis. Scientific reports 4 30127502
2016 Identification of a novel protein interaction between Elmo1 and Cdc27. Biochemical and biophysical research communications 4 26882976
2005 CDC27 protein is involved in radiation response in squamous cell cervix carcinoma. Indian journal of biochemistry & biophysics 4 23923534
2022 Circular RNA circSLC8A1 inhibits the proliferation and invasion of glioma cells through targeting the miR-214-5p/CDC27 axis. Metabolic brain disease 3 35098413
2025 Inhibition of CDC27 O-GlcNAcylation coordinates the antitumor efficacy in multiple myeloma through the autophagy-lysosome pathway. Acta pharmacologica Sinica 2 39984622
2023 Mad2B forms a complex with Cdc20, Cdc27, Rev3 and Rev1 in response to cisplatin-induced DNA damage. The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2 37641805
1995 Localization of the human homolog of the yeast cell division control 27 gene (CDC27) proximal to ITGB3 on human chromosome 17q21.3. Somatic cell and molecular genetics 2 8619132
2022 Nuclear-localized CTEN is a novel transcriptional regulator and promotes cancer cell migration through its downstream target CDC27. Journal of physiology and biochemistry 1 36399312
2021 Corrigendum: CDC27 Promotes Tumor Progression and Affects PD-L1 Expression in T-Cell Lymphoblastic Lymphoma. Frontiers in oncology 1 33585203
2026 Genetic polymorphisms of the CDC27 gene are associated with susceptibility and outcomes of non-syndromic congenital heart disease: a bi-ethnic case-control study in Chinese populations. Frontiers in genetics 0 41757267
2025 Studies on the regulation of E3 ubiquitin ligase APC3 and its interacting proteins on the tetraspore formation and release in Gracilariopsis lemaneiformis (Rhodophyta). Plant science : an international journal of experimental plant biology 0 40169068
2025 Upregulation of TFDP1 and CDC27 Plays an Important Role in Bronchiectasis. Canadian respiratory journal 0 41458288