Affinage

NONO

Non-POU domain-containing octamer-binding protein · UniProt Q15233

Length
471 aa
Mass
54.2 kDa
Annotated
2026-06-10
100 papers in source corpus 59 papers cited in narrative 59 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NONO (p54nrb) is a DBHS-family nuclear RNA/DNA-binding protein that couples transcription to RNA processing and serves as a multifunctional gene-regulatory scaffold (PMID:8371983, PMID:12358429). Defined by tandem RNA recognition motifs and a DBHS segment shared with the splicing factor PSF/SFPQ, it functions as an obligatory homodimer or as a PSF/SFPQ heterodimer, with crystallographic and NMR analyses establishing the homodimeric architecture and showing that RRM1 alone engages 5' splice-site RNA (PMID:27064654, PMID:27303796). NONO physically binds the RNA polymerase II CTD (both phosphoforms) and 5' splice sites within elongation-competent complexes containing snRNPs and elongation factors, providing a physical bridge between transcription and pre-mRNA splicing, 3'-end cleavage/polyadenylation, and termination, the last through recruitment of the exonuclease XRN2 (PMID:12358429, PMID:15057275, PMID:16373496, PMID:17639083). It is an essential structural component of paraspeckles, nuclear bodies whose assembly depends on the NEAT1 (MENε/β) lncRNA that NONO binds and stabilizes; within these bodies and an associated matrin-3/PSF complex, NONO retains inosine-hyperedited and inverted-Alu (IRAlu)-containing mRNAs at the nuclear matrix to control their export (PMID:11525732, PMID:11790299, PMID:18497743, PMID:19716791, PMID:19188602). NONO additionally acts as a sequence-specific DNA-binding transcriptional regulator: it represses nuclear hormone receptors such as the progesterone receptor by recruiting Sin3A/HDAC, and coactivates SF-1- and CREB/TORC-dependent transcription by bridging activators to pol II (PMID:19423654, PMID:11897684, PMID:18077367). In genome maintenance it forms, as an SFPQ·NONO complex, a Ku-cooperating synaptic pre-ligation complex that reconstitutes non-homologous end joining and is required for efficient double-strand-break repair in cells (PMID:15590677, PMID:25998385, PMID:19759212). Its diverse activities are gated by post-translational modification, including mitotic CDK1 phosphorylation of Thr15 that suppresses RNA binding, CARM1 methylation of the coiled-coil that reduces IRAlu-mRNA binding, GCN5/SIRT1-reversible acetylation of Lys371 controlling promoter occupancy, and caspase-2 cleavage at Asp422 that disrupts its C-terminal DNA-binding region (PMID:21819346, PMID:25792598, PMID:35843094, PMID:35444189).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1993 Medium

    Established NONO's molecular identity, defining it as a tandem-RRM protein closely related to PSF within a conserved DBHS segment, predicting a role in pre-mRNA splicing.

    Evidence Protein purification, cDNA cloning and sequence analysis from HeLa cells

    PMID:8371983

    Open questions at the time
    • Functional role inferred from homology, not yet demonstrated
    • No partner or substrate identified
  2. 1997 Medium

    Demonstrated NONO has dual transcription and splicing activities by mapping a separable N-terminal DNA-binding domain and C-terminal activation domain on a defined enhancer element.

    Evidence Protein purification, reporter gene assay in cell extracts, domain mapping

    PMID:9001221

    Open questions at the time
    • In vitro/extract-based; physiological promoter targets unclear
    • Disease relevance of the TFE3 fusion not mechanistically resolved
  3. 2002 High

    Provided the physical basis for transcription-splicing coupling by showing NONO/PSF bind the pol II CTD and snRNAs while simultaneously contacting RNA, and identified paraspeckles as a NONO-resident nuclear compartment.

    Evidence CTD affinity chromatography and co-IP with pol II holoenzyme; RNA SELEX/filter binding to U5 snRNA; live-cell imaging and FRAP

    PMID:11790299 PMID:12358429 PMID:12403470

    Open questions at the time
    • Whether CTD binding is direct or complex-mediated not fully resolved
    • Paraspeckle function not yet defined at this stage
  4. 2001 High

    Defined a nuclear-retention function: a NONO/PSF/matrin-3 complex anchors hyperedited inosine-containing RNAs to the nuclear matrix to gate their export.

    Evidence Nuclear extract fractionation, RNA affinity chromatography, co-IP, nuclear matrix fractionation

    PMID:11525732

    Open questions at the time
    • NONO's specific contribution within the trimeric complex not isolated
    • Selectivity for edited vs. unedited transcripts mechanism incomplete
  5. 2004 High

    Extended NONO beyond RNA metabolism into genome maintenance by showing the PSF·p54nrb heterodimer reconstitutes NHEJ and cooperates with Ku in a pre-ligation complex.

    Evidence In vitro NHEJ reconstitution with defined components and complex purification

    PMID:15590677

    Open questions at the time
    • In vivo requirement not yet shown at this stage
    • Mechanism of synapsis vs. ligase stimulation not separated
  6. 2009 High

    Anchored paraspeckle assembly to a specific lncRNA, showing NEAT1 is required for paraspeckle formation and that NONO selectively binds/stabilizes the NEAT1_2 isoform, linking the body to IRAlu-mRNA retention.

    Evidence NEAT1 siRNA/ASO knockdown, RNA-IP, immunofluorescence, hESC differentiation, nuclear/cytoplasmic fractionation

    PMID:18497743 PMID:19188602 PMID:19716791

    Open questions at the time
    • Stoichiometry of NONO on NEAT1 not defined
    • How editing status is sensed to select retained transcripts unresolved
  7. 2009 High

    Established NONO as a PSF-independent transcriptional corepressor of nuclear hormone receptors, directly binding the progesterone receptor and recruiting mSin3A to silence target genes.

    Evidence Co-IP, luciferase reporter, ChIP, siRNA knockdown, mutagenesis

    PMID:19423654

    Open questions at the time
    • Breadth of receptor targets beyond PR not mapped
    • Switch between corepressor and coactivator modes not defined
  8. 2009 High

    Confirmed an in vivo genome-protective role by showing NONO depletion delays DSB repair and increases radiosensitivity and chromosomal aberrations.

    Evidence siRNA/miRNA knockdown, gamma-H2AX foci, clonogenic survival, chromosomal aberration analysis

    PMID:19759212

    Open questions at the time
    • Repair pathway choice (NHEJ vs. other) in cells not delineated
    • Relationship to its RNA functions in repair unclear
  9. 2007 High

    Mechanistically connected NONO to transcription termination and 3'-end metabolism, showing it bridges XRN2 to genes and is required for downstream-RNA degradation and termination.

    Evidence Co-IP, in vitro 3' processing assay, siRNA knockdown, ChIP

    PMID:17639083

    Open questions at the time
    • Direct vs. PSF-mediated XRN2 contact not separated
    • Genome-wide scope of termination defects not assessed
  10. 2016 High

    Provided structural definition of the dimer and RNA-binding module, establishing NONO as an obligate homodimer and identifying RRM1 as the 5' splice-site-binding domain regulated by Thr15 phosphorylation.

    Evidence X-ray crystallography (2.6 Å) and SAXS of the homodimer; NMR of RRM1 with fluorescence-quenching affinity measurement

    PMID:27064654 PMID:27303796

    Open questions at the time
    • Structure of full-length protein with RNA not solved
    • Heterodimer (with PSF) interface not crystallized here
  11. 2015 High

    Showed that arginine methylation gates the nuclear-retention function, with CARM1 methylating the coiled-coil to reduce IRAlu-mRNA binding and suppress NEAT1, promoting export under stress.

    Evidence In vitro methylation assay, co-IP, RNA-IP, NEAT1 knockdown, poly(I:C) treatment, fractionation

    PMID:25792598

    Open questions at the time
    • Site-resolution of methylated residues incomplete
    • Stress-signal-to-CARM1 link not defined
  12. 2011 High

    Defined cell-cycle and phosphatase control of NONO activity: CDK1 phosphorylation of Thr15 abolishes RNA binding during mitosis while PP1 dephosphorylation reprograms its corepressor and splicing functions.

    Evidence In vitro CDK1 kinase and RNA-binding assays with T15 mutagenesis; PP1 co-IP with RVxF mutagenesis and splicing/reporter assays

    PMID:21566083 PMID:21819346

    Open questions at the time
    • Identity of the N-terminal-region kinase not established
    • How NEAT1 binding escapes phospho-regulation not explained
  13. 2018 High

    Placed NONO/paraspeckles upstream of CARM1 and chromatin marking in early development, showing p54nrb is required for CARM1 paraspeckle association, H3R26 methylation, and proper cell-fate determination.

    Evidence Immunofluorescence in mouse embryos, p54nrb depletion, H3R26 methylation analysis, developmental-arrest phenotype

    PMID:30550788

    Open questions at the time
    • Direct vs. paraspeckle-structural role in CARM1 recruitment unresolved
    • Target genes governing the Cdx2 phenotype not fully mapped
  14. 2013 High

    Demonstrated tissue-specific transcriptional and splicing control by NONO at a defined locus, showing it occupies the rhodopsin enhancer with NRL/CRX and is required for rod survival in vivo.

    Evidence ChIP-seq, co-IP, reporter assay, shRNA knockdown in mouse retina, RNA-seq

    PMID:24301678

    Open questions at the time
    • Generality of enhancer occupancy across cell types not addressed
    • Coupling of its transcription vs. splicing roles at the locus not separated
  15. 2022 Medium

    Identified additional PTM-based gating of DNA-binding activity, with GCN5/SIRT1-reversible Lys371 acetylation controlling promoter recruitment and caspase-2 cleavage at Asp422 disrupting the C-terminal DNA-binding region during cell death.

    Evidence Site-directed mutagenesis, in vitro acetylation/caspase-cleavage assays, ChIP, reporter and cell-death assays

    PMID:35444189 PMID:35843094

    Open questions at the time
    • Physiological signals driving acetylation/cleavage not defined
    • Whether modifications co-occur or act independently unclear
  16. 2025 Medium

    Revealed a phase-separation-based mechanism in which NONO co-condenses with nuclear GYS1 and MyoD/PIC components to form transcriptional condensates governing myogenic gene expression and metabolism in vivo.

    Evidence Co-IP, FRAP, in vitro phase separation, Nono-knockout mice, C2C12 differentiation and muscle-regeneration models, ChIP

    PMID:40200092

    Open questions at the time
    • Single lab; condensate requirement for transcription not genetically dissected
    • Relationship of GYS1 condensates to paraspeckles unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NONO's many activities — paraspeckle scaffolding, transcription-splicing coupling, NHEJ, and condensate formation — are coordinately partitioned within a single cell, and how the homodimer/heterodimer choice and PTM code select among them, remains unresolved.
  • No integrated model coupling structural states to functional outcomes
  • Heterodimer vs. homodimer functional division of labor undefined
  • Causal link between specific PTMs and each downstream activity in vivo incomplete

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 8 GO:0140110 transcription regulator activity 5 GO:0003677 DNA binding 4 GO:0005198 structural molecule activity 4 GO:0060090 molecular adaptor activity 4
Localization
GO:0005634 nucleus 3 GO:0005654 nucleoplasm 3 GO:0005730 nucleolus 1
Pathway
R-HSA-8953854 Metabolism of RNA 8 R-HSA-74160 Gene expression (Transcription) 5 R-HSA-73894 DNA Repair 4 R-HSA-9609507 Protein localization 4
Partners
KUMATRIN-3NEAT1PSPC1RNA POLYMERASE II CTDSFPQSIN3AXRN2
Complex memberships
NONO/PSF/matrin-3 nuclear-retention complexSFPQ·NONO (DBHS) complexparaspecklesnRNP-free U1A (SF-A) polyadenylation complex

Evidence

Reading pass · 59 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 p54nrb was purified from HeLa cells and found to contain two RNA recognition motifs (RRMs). It shares 71% identity with splicing factor PSF within a 320 aa region including both RRMs, and both p54nrb and PSF define a novel conserved protein segment termed the DBHS domain (Drosophila behavior, human splicing), suggesting involvement in pre-mRNA splicing regulation. Protein purification, cDNA cloning, sequence analysis Nucleic acids research Medium 8371983
1996 p54nrb was identified as a binding partner of the transcription factor Spi-1/PU.1 via an immobilized protein binding assay. Spi-1/PU.1 impedes the binding of p54nrb to RNA and alters the splicing process in vitro. Immobilized protein binding assay, in vitro splicing assay The Journal of biological chemistry Medium 8626664
1997 p54nrb (NonO) was found fused to the TFE3 helix-loop-helix transcription factor gene in papillary renal cell carcinoma via an X chromosome inversion inv(X)(p11.2;q12), resulting in fusion of almost the entire splicing factor protein to the TFE3 DNA-binding domain. Cytogenetic and molecular analysis of tumor translocation/inversion Oncogene Medium 9393982
1997 p54nrb was shown to bind to the IAP proximal enhancer (IPE) DNA element and to transactivate a reporter gene in HeLa cell extracts. The DNA-binding domain maps to the N-terminal half and the activation domain to the C-terminal half. p54nrb also binds pre-mRNA, demonstrating dual roles in transcription and splicing. Protein purification, reporter gene assay in cell extracts, domain mapping Molecular and cellular biology Medium 9001221
2000 PSF/p54nrb stimulates DNA topoisomerase I to jump between separate DNA helices without affecting the cleavage or religation half-reactions themselves, suggesting PSF/p54nrb mobilizes topoisomerase I after ligation. In vitro topoisomerase I jumping assay with oligonucleotide substrates Biochemistry Medium 10858305
2000 NonO/p54nrb was found to have carbonic anhydrase (CA) activity. Recombinant NonO generated in baculovirus bound to a CA inhibitor affinity column and revealed detectable CA activity (25 units/mg), identifying it as a nonclassical nuclear carbonic anhydrase. CA inhibitor affinity chromatography, CA activity measurements, recombinant protein expression in baculovirus The Journal of biological chemistry Medium 10821857
2001 A multiprotein complex containing p54nrb, PSF, and matrin 3 binds specifically to inosine-containing (hyperedited) RNAs. This complex anchors hyperedited RNAs to the nuclear matrix, mediating their nuclear retention while allowing selectively edited mRNAs to be exported. HeLa nuclear extract fractionation, RNA affinity chromatography, Co-IP, nuclear matrix fractionation Cell High 11525732
2001 p54nrb is cleaved/modified during Fas-induced apoptosis, as identified by 2D-gel proteome analysis of apoptotic Jurkat T cells with calculated caspase-3 cleavage sites. 2D-PAGE proteomics, MALDI-TOF mass spectrometry, recombinant caspase-3 treatment The Journal of biological chemistry Low 11352910
2001 PSF functions as a novel corepressor of nuclear hormone receptors (TR, RXR) by interacting with Sin3A and mediating silencing through recruitment of HDACs to the receptor DNA-binding domain. NonO/p54nrb was co-purified with PSF in this complex on TR and RXR, though p54nrb's independent contribution was not fully separated from PSF's. Biochemical purification, co-immunoprecipitation, reporter gene assay, in vivo studies with TR Molecular and cellular biology Medium 11259580
2002 p54nrb localizes to a novel nuclear compartment termed paraspeckles (10–20 per cell), which are discrete bodies in the interchromatin nucleoplasmic space often adjacent to splicing speckles. p54nrb interacts dynamically with nucleoli in a transcription-dependent fashion and relocalizes to nucleolar cap structures when transcription is inhibited. Immunofluorescence, YFP-PSP1 stable cell line live imaging, FRAP, fluorescence microscopy Current biology : CB High 11790299
2002 PSF and p54nrb/NonO bind specifically to the RNA polymerase II CTD (both hypo- and hyperphosphorylated forms) via affinity chromatography and co-immunoprecipitate with pol II holoenzyme. They can simultaneously interact with CTD and RNA, providing a physical link between pol II and pre-mRNA processing. CTD affinity chromatography, co-immunoprecipitation, RNA-binding assay RNA (New York, N.Y.) High 12358429
2002 PSF and p54nrb interact with each other and bind U5 snRNA stem 1b via iterative selection and filter-binding assays. Both proteins associate with spliceosomes and the U4/U6.U5 tri-snRNP. RNA SELEX, filter-binding assay, RNA affinity selection, sedimentation analysis RNA (New York, N.Y.) Medium 12403470
2002 p54nrb and PSF form complexes with SF-1 on the CYP17 promoter. The SF-1/PSF/p54nrb/NonO complex is required for cAMP-dependent transcriptional activation of the CYP17 gene. SF-1 co-immunoprecipitates with p54nrb indicating direct interaction. PSF-recruited mSin3A and HDAC activity mediates repression of basal transcription. EMSA, co-immunoprecipitation, luciferase reporter assay, trichostatin A treatment Endocrinology Medium 11897684
2004 p54nrb associates with the 5' splice site within large complexes in HeLa cell nuclear extracts containing hyperphosphorylated RNA pol II (RNAPIIO) together with U1 or U1/U2 snRNPs, P-TEFb, TAT-SF1, TFIIF, PSF, and TLS. p54nrb and PSF can directly interact with the 5' splice site, suggesting they mediate contacts between RNAPII and snRNPs during coupled transcription/splicing. Nuclear extract fractionation, RNA-protein binding assay, immobilized DNA template assay The EMBO journal Medium 15057275
2004 PSF and p54nrb form a stable heteromeric complex that reconstitutes efficient DNA double-strand break rejoining in vitro when added to the other five known NHEJ polypeptides. The PSF·p54nrb complex cooperates with Ku protein to form a functional preligation complex with substrate DNA. In vitro NHEJ reconstitution assay, complex purification The Journal of biological chemistry High 15590677
2005 p54nrb forms a heterodimer with PSP1 in vivo, and this interaction requires a specific domain within PSP1. The PSP1-p54nrb interaction is necessary but not sufficient for PSP1 paraspeckle targeting, which also requires an RNA-binding-capable RRM. Paraspeckle formation depends on RNA Pol II transcription. Co-immunoprecipitation, domain mapping by truncation mutants, DRB transcription inhibition, fluorescence microscopy Molecular biology of the cell High 16148043
2005 p54nrb is multiphosphorylated during mitosis. CDK1 (identified via roscovitine inhibition and cyclin B1 immunodepletion) phosphorylates the C-terminal extremity of p54nrb. A different kinase phosphorylates the N-terminal region. p54nrb interacts with the peptidylprolyl isomerase Pin1 via three C-terminal threonine residues, with binding favored when at least two are phosphorylated, suggesting regulation by phosphorylation-dependent conformational changes. Phospho-specific antibody staining, CDK inhibitor treatment, immunodepletion, site-directed mutagenesis, Pin1 interaction assay Journal of molecular biology High 15701524
2005 PSF and p54nrb preferentially bind strong transcriptional activation domains and preferentially bind the full-length pol II CTD (52 repeats) rather than a truncated 15-repeat CTD. Elevated PSF expression bypasses the requirement for a strong activator for efficient splicing and 3'-end cleavage. PSF/p54nrb-dependent stimulation of splicing primarily affects first intron removal. Affinity chromatography, transfection/overexpression, reporter assay, CTD truncation mutants Molecular and cellular biology Medium 16024807
2006 p54nrb is a component of the snRNP-free U1A (SF-A) complex that also contains PSF and p68. Immunodepletion of p54nrb followed by reconstitution experiments showed that p54nrb is critical for pre-mRNA cleavage during polyadenylation. TAP purification, mass spectrometry, immunodepletion, reconstitution, in vitro polyadenylation assay RNA (New York, N.Y.) High 16373496
2006 Nuclear N-WASP exists in a large nuclear complex containing PSF-NonO/p54nrb, nuclear actin, and RNA polymerase II. The interaction of N-WASP with the PSF-NonO complex couples N-WASP to RNA pol II to regulate transcription. N-WASP-mediated nuclear actin polymerization contributes to this regulation. Co-immunoprecipitation, nuclear complex purification, transcription reporter assay Nature cell biology Medium 16767080
2007 p54nrb/NonO is required for cAMP-dependent activation of CREB target genes in vivo. TORC2 and NONO complex on cAMP-responsive promoters, and NONO acts as a bridge between the CREB/TORC complex and RNA polymerase II. High-throughput coactivator trap screen, RNAi knockdown, chromatin immunoprecipitation (ChIP) Proceedings of the National Academy of Sciences of the United States of America Medium 18077367
2007 p54nrb/NonO physically associates with XRN2 via PSF/p54nrb acting as a bridge. p54nrb is present along the length of genes, is required for degradation of the 3'-cleaved RNA downstream of the poly(A) site in vitro, and its siRNA knockdown leads to defects in XRN2 recruitment and transcription termination in vivo. Co-immunoprecipitation, in vitro 3' processing assay, siRNA knockdown, ChIP Genes & development High 17639083
2007 PSF and p54nrb bind RNAs containing AU-rich elements (AREs), including the TNFα mRNA. PSF is phosphorylated by Mnk kinases at two sites in vitro and in vivo, and Mnk-mediated phosphorylation increases the binding of PSF to TNFα mRNA in living cells. In vitro kinase assay, mass spectrometry, RNA immunoprecipitation (RIP), cap-resin pulldown The Journal of biological chemistry Medium 17965020
2007 p54nrb and PSF interact with the two COX-2 upstream sequence elements (USEs). Tethering p54nrb to COX-2 USE mutant RNA compensates for the cis-acting elements in polyadenylation. p54nrb, PTB, PSF, and U1A may interact as a complex since co-immunoprecipitation of MS2 fusion proteins coprecipitates the other partners. RNA pulldown with biotinylated oligonucleotides, MS2 tethering assay, co-immunoprecipitation RNA (New York, N.Y.) Medium 17507659
2007 p54nrb physically interacts with Sox9 and enhances Sox9-dependent transcriptional activation of the Col2a1 promoter. p54nrb colocalizes with Sox9 in nuclear paraspeckle bodies in ATDC5 cells. A p54nrb mutant lacking RNA recognition motifs dominantly inhibits Col2a1 mRNA maturation, chondrocyte differentiation in vitro and in mesenchymal explants, and causes dwarfism in transgenic mice. Co-immunoprecipitation, reporter assay, dominant-negative RRM mutant overexpression, transgenic mouse model, RNA maturation assay The Journal of clinical investigation High 18677406
2008 p54nrb binds both BRG1 and Brm (catalytic subunits of the SWI/SNF complex) and the core subunit BAF60a. The N-terminal region of p54nrb is sufficient to pull down other SWI/SNF core subunits. Brm in concert with p54nrb co-localizes at a TERT alternative splicing acceptor site and modulates TERT splicing (promoting exon-inclusion) and transcription. Pulldown assay, co-immunoprecipitation, shRNA knockdown, ChIP, RT-PCR splicing analysis The Biochemical journal Medium 18042045
2008 mRNAs containing inverted Alu repeats in their 3'-UTR are retained in the nucleus and associate with p54nrb in a manner that correlates with A-to-I RNA editing. The Nicolin 1 mRNA isoform containing inverted Alu repeats is retained in the nucleus while the isoform lacking these sequences is exported, identifying p54nrb as a component of a gene silencing mechanism mediated by Alu elements. EGFP reporter assay, nuclear/cytoplasmic fractionation, RNA immunoprecipitation The EMBO journal Medium 18497743
2009 p54nrb/PSF and PSP1 are expressed in human embryonic stem cells but paraspeckles are absent; paraspeckle assembly requires NEAT1 lncRNA expression, which is absent in hESCs and induced upon differentiation. Knockdown of NEAT1 in HeLa cells results in loss of paraspeckles and enhanced cytoplasmic export of mRNAs containing inverted Alu repeats. siRNA knockdown of NEAT1, immunofluorescence, nuclear/cytoplasmic fractionation, hESC differentiation Molecular cell High 19716791
2009 p54/nrb and PSF selectively associate with and stabilize the longer MENbeta (NEAT1_2) lncRNA isoform among the two paraspeckle ncRNAs (MENepsilon/MENbeta), contributing to paraspeckle structural organization. Removal of MENepsilon/beta by knockdown results in paraspeckle disintegration. RNA immunoprecipitation, siRNA knockdown, immunofluorescence Proceedings of the National Academy of Sciences of the United States of America High 19188602
2009 p54nrb contributes to rapid and accurate repair of DNA double-strand breaks in vivo. siRNA-mediated p54nrb knockdown delays DSB repair (gamma-H2AX focus assay) and stable miRNA knockdown cell lines show increased ionizing radiation-induced chromosomal aberrations and increased radiosensitivity in clonogenic assay. siRNA/miRNA knockdown, gamma-H2AX focus assay, clonogenic survival assay, chromosomal aberration analysis Nucleic acids research High 19759212
2009 p54nrb interacts with hnRNP M via co-immunoprecipitation and pulldown assays, co-localizes with PSF in a subset of nuclear paraspeckles, and co-fractionates with PSF and p54nrb in the nuclear matrix. PSF and hnRNP M have opposing effects on alternative splicing: hnRNP M promotes exon skipping while PSF favors inclusion, and their antagonism requires hnRNP M domains capable of interacting with PSF/p54nrb. Co-immunoprecipitation, pulldown, immunofluorescence, nuclear matrix fractionation, PPT minigene splicing assay Experimental cell research Medium 19874820
2009 p54nrb is a transcriptional corepressor of the progesterone receptor (PR) independent of PSF. p54nrb interacts directly with PR in a ligand-independent manner. p54nrb recruits mSin3A through its N-terminus to the PR-DNA complex, resulting in inhibition of PR-mediated transactivation. Knockdown of p54nrb alleviates PR-mediated repression on connexin 43 (Gja1) transcription. Co-immunoprecipitation, luciferase reporter assay, ChIP, siRNA knockdown, site-directed mutagenesis Molecular endocrinology (Baltimore, Md.) High 19423654
2011 Mitotic CDK1-mediated phosphorylation of p54nrb reduces its general RNA-binding ability. In vitro CDK1 phosphorylation of GST-p54nrb abolishes interaction with 5' splice site RNA and homoribopolymers poly(A), poly(C), and poly(U) but not poly(G). Threonine 15 (N-terminal to the tandem RRM domains) is required for this inhibition by mutagenesis. Neat1 ncRNA co-immunoprecipitates with p54nrb in both interphase and mitotic cells, indicating that p54nrb-Neat1 interaction is not modulated by phosphorylation. In vitro kinase assay (CDK1), RNA binding assay, site-directed mutagenesis (T15), co-immunoprecipitation, cell synchronization Biochemistry and cell biology High 21819346
2011 PP1 forms a protein complex with p54nrb and PSF, interacting directly with p54nrb via its RVxF motif. PP1 association results in dephosphorylation of p54nrb and PSF, leading to loss of their transcriptional corepressor activities and altered alternative splicing activities (shifting from exon inclusion to exon skipping). Dephosphorylation changes p54nrb/PSF interactions with Sin3A, HDAC1, U1A and U2AF. Co-immunoprecipitation, RVxF motif mutagenesis, luciferase reporter assay, CD44 minigene splicing assay, in vivo phosphorylation analysis Molecular endocrinology (Baltimore, Md.) High 21566083
2012 p54nrb forms a complex with ILF3 that binds to the survivin promoter to regulate survivin expression. The small molecule YM155 binds ILF3 and disrupts the ILF3/p54nrb complex, resulting in different subcellular localization of the two proteins and suppression of survivin expression. Co-immunoprecipitation, ChIP, immunofluorescence localization, YM155 treatment Biochemical and biophysical research communications Medium 22842455
2013 NonO/p54nrb binds to the rhodopsin distal enhancer region (RER) as validated by ChIP-seq showing high NonO occupancy at rhodopsin and phototransduction genes. NonO and its interactors (hnRNP M, Ywhaz, Ppp1ca) activate the rhodopsin promoter in HEK293 cells synergistically with NRL and CRX; the DNA-binding domain of NonO is critical. shRNA knockdown of NonO in mouse retina leads to loss of rhodopsin expression, rod cell death, splicing defects, and altered expression of phototransduction genes. Mass spectrometry identification, co-immunoprecipitation, ChIP-seq, luciferase reporter assay, shRNA knockdown in retina, RNA-seq Human molecular genetics High 24301678
2013 During apoptosis induced by TRAIL, PTB forms a remodeled complex with PSF and NONO/p54nrb among others. Increased expression of NONO/p54nrb accelerates apoptosis. These proteins post-transcriptionally regulate apoptosis rates by interacting with and stimulating IRES activity in mRNAs translated during apoptosis. Co-immunoprecipitation, overexpression/knockdown with apoptosis rate measurement, IRES reporter assay Cell death and differentiation Medium 24141718
2014 p54nrb/NonO and PSF promote U snRNA nuclear export by accelerating assembly of the U snRNA export complex. As a heterodimer, they accelerate the recruitment of PHAX, and subsequently CRM1 and Ran, onto RNA substrates in vitro, mediating efficient U snRNA export in vivo. In vitro U snRNA export complex assembly assay, microinjection/in vivo export assay Nucleic acids research Medium 24413662
2015 CARM1 (coactivator-associated arginine methyltransferase 1) methylates the coiled-coil domain of p54nrb, resulting in reduced binding of p54nrb to mRNAs containing inverted Alu repeats (IRAlus). CARM1 also suppresses NEAT1 transcription to reduce paraspeckle formation. Both mechanisms together promote nuclear export of IRAlu-containing mRNAs, especially under poly(I:C) stress. Co-immunoprecipitation, in vitro methylation assay, RNA immunoprecipitation, NEAT1 knockdown, poly(I:C) treatment, nuclear/cytoplasmic fractionation Genes & development High 25792598
2015 The SFPQ·NONO native complex purified from HeLa cells binds DNA independently of free ends (unlike Ku), has DNA pairing activity in a microwell assay, and stimulates DNA-dependent protein kinase (DNA-PKcs) autophosphorylation. These findings suggest SFPQ·NONO promotes end joining by binding internal DNA sequences and stabilizing a synaptic pre-ligation complex without occluding DNA termini. Native complex purification from HeLa cells, microwell-based DNA binding/pairing assay, DNA-PK autophosphorylation assay Biochemical and biophysical research communications Medium 25998385
2015 p54nrb/NONO binds the nuclear form of SREBP-1a, and the conserved Y267 residue of p54nrb is required for this interaction. p54nrb binding to nuclear SREBP-1a increases its protein stability, stimulating SREBP-1-mediated transcription of lipogenic genes and lipid production in breast cancer cells. Co-immunoprecipitation, site-directed mutagenesis (Y267), reporter assay, in vitro and in vivo tumor growth assays Oncogene Medium 26148231
2015 p54nrb/NONO regulates glucocorticoid production by modulating the splicing of multiple phosphodiesterase (PDE) isoforms and facilitating the interaction between XRN2 and select PDE transcripts to regulate their stability. Silencing p54nrb in H295R adrenocortical cells decreases cAMP production and cortisol biosynthesis in response to ACTH. siRNA knockdown, RT-PCR splicing analysis, RNA stability assay, co-immunoprecipitation Molecular and cellular biology Medium 25605330
2015 p54nrb/NONO transcriptionally regulates the lncRNA PCGEM1 by binding its promoter in response to androgen deprivation. Suppression of p54nrb reduces PCGEM1, and re-expression restores it. DIM (3,3'-diindolylmethane) suppresses PCGEM1 by preventing the interaction of p54nrb with the PCGEM1 promoter. siRNA/CRISPR KO, rescue experiments, ChIP showing p54nrb on PCGEM1 promoter Scientific reports Medium 27682980
2016 Progesterone-liganded nuclear PRB forms a complex including JUN/JUN homodimers and P54nrb/Sin3A/HDAC to repress transcription of the labor gene Cx43. Unliganded PRA paradoxically activates Cx43 transcription. Increased 20α-HSD expression reduces nuclear progesterone levels during labor, switching PR from liganded to unliganded state. Co-immunoprecipitation, ChIP, reporter assay, siRNA knockdown, immunofluorescence Nature communications Medium 27220952
2016 The structure of p54nrb/NonO RRM1 was determined by NMR, revealing a canonical fold (β1α1β2β3α2β4) with conserved aromatic amino acids. Only RRM1 (not RRM2) binds to 5' splice site RNA. The binding interface was mapped by NMR and the affinity was determined by fluorescence quenching. Phosphorylation of T15 (N-terminal to RRM1) inhibits RNA binding except for G-rich RNAs. NMR structure determination, fluorescence quenching binding assay, in vitro RNA binding Biochemistry High 27064654
2016 Crystal structure of NONO homodimer was obtained at 2.6 Å resolution by molecular replacement. NONO crystallizes as a superhelical arrangement of six NONO homodimers, establishing that NONO is an obligatory homodimer. L-proline stabilizes purified NONO homodimers and prevents aggregation. X-ray crystallography, SAXS Acta crystallographica. Section D, Structural biology High 27303796
2016 Tyrosine residues of p54nrb are not phosphorylated (p54nrb shows non-specific binding to anti-phosphotyrosine antibodies). However, replacement of tyrosine residues with phenylalanine altered p54nrb activities in transcriptional co-repression and RNA splicing in a gene context-dependent manner by differentially regulating p54nrb protein associations with interacting partners. Site-directed mutagenesis (Y-to-F substitutions), luciferase reporter assay, minigene splicing assay, co-immunoprecipitation, confocal microscopy Journal of cellular physiology Medium 27430900
2017 NEAT1 depletion releases sequestered P54nrb and PSF from paraspeckles to facilitate IRES-dependent translation of c-Myc mRNA. Increased association of P54nrb and PSF with c-Myc mRNA was observed in NEAT1-depleted cells under RNAP I inhibition (CX5461 treatment). Antisense oligonucleotide-mediated NEAT1 knockdown, RNA immunoprecipitation (RIP), immunofluorescence PloS one Medium 28288210
2018 CARM1 accumulates in nuclear granules (mostly paraspeckles) at the 2- to 4-cell stage of mouse embryo development. The paraspeckle component NEAT1 and its partner p54nrb are required for CARM1's association with paraspeckles and for H3R26 methylation. Conversely, CARM1 influences paraspeckle organization. Depletion of p54nrb results in arrest at the 16- to 32-cell stage with elevated Cdx2, indicating that paraspeckles (with p54nrb as a component) act upstream of CARM1 in early embryo cell fate determination. Immunofluorescence in mouse embryos, p54nrb depletion (siRNA/morpholino), H3R26 methylation analysis, developmental arrest assay Cell High 30550788
2019 P54nrb interacts with RNase H1 via its core domains; toxic phosphorothioate antisense oligonucleotides (PS-ASOs) form a complex that includes RNase H1, P54nrb, and PSF (but safe PS-ASOs do not). P54nrb RRM1 and RRM2 are required for PS-ASO binding. The protein-protein interaction between P54nrb and RNase H1 requires the spacer region of RNase H1. RNA is also a required component of this complex. NanoLuciferase structural complementation reporter (real-time in live cells), in vitro binding assay, domain deletion analysis Nucleic acids research Medium 31495875
2020 NONO promotes BIN1 exon 12a inclusion (oncogenic splicing switch) through interaction with DHX9 and SFPQ forming a DHX9-NONO-SFPQ complex. Knockdown of NONO abolishes liver cancer cell proliferation, migration, and BIN1-L expression, while BIN1-L stabilizes PLK1 by preventing cullin 3-mediated ubiquitination. RNA-seq, co-immunoprecipitation, siRNA knockdown, minigene splicing assay, tumor formation assay Hepatology (Baltimore, Md.) Medium 31815296
2020 SFPQ, in complex with p54nrb, binds and regulates the activity of splicing factor SRSF2 under platinum treatment. The SFPQ/p54nrb complex decreases SRSF2 binding to caspase-9 RNA, favoring expression of the antiapoptotic caspase-9b isoform and contributing to chemoresistance. Co-immunoprecipitation, RNA immunoprecipitation, siRNA knockdown, alternative splicing analysis Oncogene Medium 32332923
2021 NONO interacted with and stabilized both HIF-1α and HIF-2α complexes, activating transcription of hypoxia-induced genes. NONO also bound pre-mRNA and mature mRNA of HIF-1/2 targets to facilitate splicing and mRNA stability. NONO knockout disrupted expression of HIF-1/2 targets and impeded hypoxia-enhanced progression in hepatocellular carcinoma. Co-immunoprecipitation, RNA immunoprecipitation, NONO knockout, in vitro/in vivo tumor assays, ChIP Oncogene Medium 34079086
2022 p54nrb is a substrate of caspase-2, which cleaves p54nrb at D422, disrupting its C-terminal DNA-binding region. Loss or cleavage of p54nrb results in altered expression of oncogenic genes including cathepsin-Z and gelsolin. p54nrb interacts with cathepsin-Z and gelsolin DNA (but not RNA). Loss of p54nrb increases cell death susceptibility in tumor cell lines. In vitro caspase-2 cleavage assay, quantitative proteomics, ChIP (DNA interaction), siRNA/CRISPR knockdown/KO, cell death assay Cell death & disease Medium 35444189
2022 Lysine 371 of p54nrb is reversibly acetylated: acetyltransferase GCN5 acetylates K371, and deacetylase SIRT1 removes the modification. GCN5-mediated acetylation attenuates recruitment of p54nrb to its core binding motif within the IL-8 gene promoter, increasing IL-8 expression; SIRT1-mediated deacetylation reverses this. Site-directed mutagenesis (K371), co-immunoprecipitation, ChIP, luciferase reporter assay, in vitro acetylation assay Biochemical and biophysical research communications Medium 35843094
2022 p54nrb/NONO significantly interacted with nuclear EGFR in triple-negative breast cancer. NONO increased the stability of nuclear EGFR and recruited CBP/p300, enhancing EGFR transcriptional activity. In turn, EGFR positively regulated NONO's affinity to mRNAs of EGFR downstream genes. Co-immunoprecipitation, proximity ligation assay, siRNA knockdown, reporter assay, protein stability assay Cell death & disease Medium 35013116
2022 Paraspeckle proteins p54nrb and PSPC1 function as IRES trans-acting factors (ITAFs) for subgroups of (lymph)angiogenic and cardioprotective factor mRNAs during hypoxia. NEAT1 and IRES-containing mRNAs are recruited into paraspeckles during hypoxia. Paraspeckles act as a platform for IRESome assembly and IRES-dependent translation. siRNA knockdown, polysome profiling, smiFISH for mRNA localization, mass spectrometry, IRES reporter assay eLife Medium 36546462
2024 Nono deletion in murine KP lung cancer cells impairs the DNA damage response to etoposide and bleomycin, with hyperactivation of DSB signaling, reduced RNA pol II promoter occupancy, impaired nascent RNA synthesis, and attenuated induction of Gadd45b. Gadd45b is identified as a putative Nono-dependent effector of the DDR, indicating Nono mediates a genome-protective crosstalk between the DDR and RNA metabolism. CRISPR/Cas9 Nono deletion, gamma-H2AX assay, Pol II ChIP, nascent RNA synthesis assay, RT-PCR/Western blot for Gadd45b Life science alliance Medium 38843934
2025 GYS1 (glycogen synthase 1) complexes with NONO/p54nrb in the nucleus and undergoes liquid-liquid phase separation with NONO to form nuclear condensates, leading to GYS1 nuclear retention and inhibition of glycogen biosynthesis. NONO and nGYS1 co-condense with MyoD and preinitiation complex (PIC) proteins to form transcriptional condensates, driving myogenic gene expression. Nono-deficient mice exhibit exercise intolerance, higher muscle glycogen content, smaller myofibers, and impaired muscle regeneration. Co-immunoprecipitation, FRAP, in vitro phase separation assay, Nono knockout mice, C2C12 differentiation assay, cardiotoxin muscle regeneration model, ChIP Cell death and differentiation Medium 40200092

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2009 Altered nuclear retention of mRNAs containing inverted repeats in human embryonic stem cells: functional role of a nuclear noncoding RNA. Molecular cell 556 19716791
2009 MENepsilon/beta noncoding RNAs are essential for structural integrity of nuclear paraspeckles. Proceedings of the National Academy of Sciences of the United States of America 524 19188602
2002 Paraspeckles: a novel nuclear domain. Current biology : CB 452 11790299
2001 The fate of dsRNA in the nucleus: a p54(nrb)-containing complex mediates the nuclear retention of promiscuously A-to-I edited RNAs. Cell 406 11525732
2002 PSF and p54(nrb)/NonO--multi-functional nuclear proteins. FEBS letters 297 12417296
1997 Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma. Oncogene 281 9393982
2008 Alu element-mediated gene silencing. The EMBO journal 280 18497743
2005 P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. Molecular biology of the cell 198 16148043
2002 Splicing and transcription-associated proteins PSF and p54nrb/nonO bind to the RNA polymerase II CTD. RNA (New York, N.Y.) 161 12358429
2004 p54(nrb) associates with the 5' splice site within large transcription/splicing complexes. The EMBO journal 156 15057275
2001 PSF is a novel corepressor that mediates its effect through Sin3A and the DNA binding domain of nuclear hormone receptors. Molecular and cellular biology 154 11259580
1993 Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6. Nucleic acids research 152 8371983
2007 The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination. Genes & development 151 17639083
2006 Regulation of RNA-polymerase-II-dependent transcription by N-WASP and its nuclear-binding partners. Nature cell biology 138 16767080
2016 Molecular evidence of functional progesterone withdrawal in human myometrium. Nature communications 125 27220952
2018 CARM1 and Paraspeckles Regulate Pre-implantation Mouse Embryo Development. Cell 113 30550788
2001 Predominant identification of RNA-binding proteins in Fas-induced apoptosis by proteome analysis. The Journal of biological chemistry 109 11352910
2002 Transcriptional activation of human CYP17 in H295R adrenocortical cells depends on complex formation among p54(nrb)/NonO, protein-associated splicing factor, and SF-1, a complex that also participates in repression of transcription. Endocrinology 106 11897684
2005 Role for PSF in mediating transcriptional activator-dependent stimulation of pre-mRNA processing in vivo. Molecular and cellular biology 105 16024807
2002 PSF and p54nrb bind a conserved stem in U5 snRNA. RNA (New York, N.Y.) 103 12403470
2004 Identification of the polypyrimidine tract binding protein-associated splicing factor.p54(nrb) complex as a candidate DNA double-strand break rejoining factor. The Journal of biological chemistry 101 15590677
2007 A coactivator trap identifies NONO (p54nrb) as a component of the cAMP-signaling pathway. Proceedings of the National Academy of Sciences of the United States of America 92 18077367
2007 Transcriptional activity of androgen receptor is modulated by two RNA splicing factors, PSF and p54nrb. Molecular and cellular biology 90 17452459
2015 Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IRAlus. Genes & development 89 25792598
1996 The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb. The Journal of biological chemistry 89 8626664
2015 2'-Fluoro-modified phosphorothioate oligonucleotide can cause rapid degradation of P54nrb and PSF. Nucleic acids research 87 25855809
2009 Involvement of p54(nrb), a PSF partner protein, in DNA double-strand break repair and radioresistance. Nucleic acids research 79 19759212
1997 The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO. Molecular and cellular biology 78 9001221
2007 Specific trans-acting proteins interact with auxiliary RNA polyadenylation elements in the COX-2 3'-UTR. RNA (New York, N.Y.) 76 17507659
2008 Paraspeckle protein p54nrb links Sox9-mediated transcription with RNA processing during chondrogenesis in mice. The Journal of clinical investigation 73 18677406
2015 p54(nrb)/NONO regulates lipid metabolism and breast cancer growth through SREBP-1A. Oncogene 70 26148231
2009 p54nrb is a transcriptional corepressor of the progesterone receptor that modulates transcription of the labor-associated gene, connexin 43 (Gja1). Molecular endocrinology (Baltimore, Md.) 70 19423654
2007 The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. The Journal of biological chemistry 69 17965020
2020 NONO and tumorigenesis: More than splicing. Journal of cellular and molecular medicine 67 32168434
2011 p54nrb is a new regulator of progression of malignant melanoma. Carcinogenesis 66 21642354
2012 Sepantronium bromide (YM155) induces disruption of the ILF3/p54(nrb) complex, which is required for survivin expression. Biochemical and biophysical research communications 65 22842455
2010 Sequences in PSF/SFPQ mediate radioresistance and recruitment of PSF/SFPQ-containing complexes to DNA damage sites in human cells. DNA repair 61 21144806
2002 Adrenocorticotropin/cyclic adenosine 3',5'-monophosphate-mediated transcription of the human CYP17 gene in the adrenal cortex is dependent on phosphatase activity. Endocrinology 61 11956159
2020 Splicing factor proline- and glutamine-rich (SFPQ) protein regulates platinum response in ovarian cancer-modulating SRSF2 activity. Oncogene 60 32332923
2003 p54nrb acts as a transcriptional coactivator for activation function 1 of the human androgen receptor. Biochemical and biophysical research communications 60 12810069
2020 Splicing Regulator p54nrb /Non-POU Domain-Containing Octamer-Binding Protein Enhances Carcinogenesis Through Oncogenic Isoform Switch of MYC Box-Dependent Interacting Protein 1 in Hepatocellular Carcinoma. Hepatology (Baltimore, Md.) 59 31815296
2013 The transcription-splicing protein NonO/p54nrb and three NonO-interacting proteins bind to distal enhancer region and augment rhodopsin expression. Human molecular genetics 49 24301678
2009 hnRNP M interacts with PSF and p54(nrb) and co-localizes within defined nuclear structures. Experimental cell research 47 19874820
2006 Coregulator exchange and sphingosine-sensitive cooperativity of steroidogenic factor-1, general control nonderepressed 5, p54, and p160 coactivators regulate cyclic adenosine 3',5'-monophosphate-dependent cytochrome P450c17 transcription rate. Molecular endocrinology (Baltimore, Md.) 47 17121866
2015 New Noncoding Lytic Transcripts Derived from the Epstein-Barr Virus Latency Origin of Replication, oriP, Are Hyperedited, Bind the Paraspeckle Protein, NONO/p54nrb, and Support Viral Lytic Transcription. Journal of virology 46 25926645
2006 p54nrb is a component of the snRNP-free U1A (SF-A) complex that promotes pre-mRNA cleavage during polyadenylation. RNA (New York, N.Y.) 43 16373496
2016 Regulation of PCGEM1 by p54/nrb in prostate cancer. Scientific reports 41 27682980
2005 The multifunctional nuclear protein p54nrb is multiphosphorylated in mitosis and interacts with the mitotic regulator Pin1. Journal of molecular biology 41 15701524
2009 The hepatitis delta virus RNA genome interacts with eEF1A1, p54(nrb), hnRNP-L, GAPDH and ASF/SF2. Virology 40 19464723
2003 55 kDa nuclear matrix protein (nmt55) mRNA is expressed in human prostate cancer tissue and is associated with the androgen receptor. International journal of cancer 40 12672026
2009 Transcriptional activity of the murine retinol-binding protein gene is regulated by a multiprotein complex containing HMGA1, p54 nrb/NonO, protein-associated splicing factor (PSF) and steroidogenic factor 1 (SF1)/liver receptor homologue 1 (LRH-1). The international journal of biochemistry & cell biology 39 19389484
2008 Brm transactivates the telomerase reverse transcriptase (TERT) gene and modulates the splicing patterns of its transcripts in concert with p54(nrb). The Biochemical journal 39 18042045
2001 Immunodetection of nmt55/p54nrb isoforms in human breast cancer. BMC cancer 39 11710964
2004 Expression and functional significance of mouse paraspeckle protein 1 on spermatogenesis. Biology of reproduction 38 15140795
2000 PSF/p54(nrb) stimulates "jumping" of DNA topoisomerase I between separate DNA helices. Biochemistry 38 10858305
2019 Kinetic and subcellular analysis of PS-ASO/protein interactions with P54nrb and RNase H1. Nucleic acids research 37 31495875
2005 Functional analysis of the promoter of the mitochondrial phosphate carrier human gene: identification of activator and repressor elements and their transcription factors. The Biochemical journal 36 15984930
2014 p54nrb/NonO and PSF promote U snRNA nuclear export by accelerating its export complex assembly. Nucleic acids research 31 24413662
2007 Bipartite nuclear localization signal of matrin 3 is essential for vertebrate cells. Biochemical and biophysical research communications 30 17223080
2013 Remodelling of a polypyrimidine tract-binding protein complex during apoptosis activates cellular IRESs. Cell death and differentiation 29 24141718
2015 Characterization of DNA binding and pairing activities associated with the native SFPQ·NONO DNA repair protein complex. Biochemical and biophysical research communications 28 25998385
2018 Overexpression of p54nrb/NONO induces differential EPHA6 splicing and contributes to castration-resistant prostate cancer growth. Oncotarget 26 29535823
2010 Enhanced cartilage regeneration in MIA/CD-RAP deficient mice. Cell death & disease 26 21368873
2007 Switched alternative splicing of oncogene CoAA during embryonal carcinoma stem cell differentiation. Nucleic acids research 26 17337438
2015 p54nrb/NONO regulates cyclic AMP-dependent glucocorticoid production by modulating phosphodiesterase mRNA splicing and degradation. Molecular and cellular biology 25 25605330
2017 Depletion of NEAT1 lncRNA attenuates nucleolar stress by releasing sequestered P54nrb and PSF to facilitate c-Myc translation. PloS one 24 28288210
2013 F11R expression upon hypoxia is regulated by RNA editing. PloS one 23 24147060
2021 Nuclear scaffold protein p54nrb/NONO facilitates the hypoxia-enhanced progression of hepatocellular carcinoma. Oncogene 22 34079086
2000 Nuclear NonO/p54(nrb) protein is a nonclassical carbonic anhydrase. The Journal of biological chemistry 22 10821857
1997 AFX1 and p54nrb: fine mapping, genomic structure, and exclusion as candidate genes of X-linked dystonia parkinsonism. Human genetics 22 9341872
2011 Consensus PP1 binding motifs regulate transcriptional corepression and alternative RNA splicing activities of the steroid receptor coregulators, p54nrb and PSF. Molecular endocrinology (Baltimore, Md.) 21 21566083
1997 Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer. Diagnostic molecular pathology : the American journal of surgical pathology, part B 21 9360842
2022 RNA-binding protein p54nrb/NONO potentiates nuclear EGFR-mediated tumorigenesis of triple-negative breast cancer. Cell death & disease 20 35013116
2022 Long non-coding RNA Neat1 and paraspeckle components are translational regulators in hypoxia. eLife 20 36546462
2012 Proteomic identification of PSF and p54(nrb) as TopBP1-interacting proteins. Journal of cellular biochemistry 19 22213094
2015 Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation. PloS one 17 26332123
2011 The mitotic phosphorylation of p54(nrb) modulates its RNA binding activity. Biochemistry and cell biology = Biochimie et biologie cellulaire 15 21819346
1998 Prolactin induces expression of FGF-2 and a novel FGF-responsive NonO/p54nrb-related mRNA in rat lymphoma cells. Molecular and cellular endocrinology 15 9605521
2016 Structure, Dynamics, and Interaction of p54(nrb)/NonO RRM1 with 5' Splice Site RNA Sequence. Biochemistry 14 27064654
2007 RRM proteins interacting with the cap region of topoisomerase I. Journal of molecular biology 14 17481653
2005 Identification of p54(nrb) and the 14-3-3 Protein HS1 as TNF-alpha-inducible genes related to cell cycle control and apoptosis in human arterial endothelial cells. Journal of biochemistry and molecular biology 14 16053712
2016 A crystallographic study of human NONO (p54(nrb)): overcoming pathological problems with purification, data collection and noncrystallographic symmetry. Acta crystallographica. Section D, Structural biology 13 27303796
2008 Proteomic identification of a PSF/p54nrb heterodimer as RNF43 oncoprotein-interacting proteins. Proteomics 13 18655028
2013 YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis. PloS one 12 24349210
2015 Subnuclear re-localization of SOX10 and p54NRB correlates with a unique neurological phenotype associated with SOX10 missense mutations. Human molecular genetics 11 26060192
2015 Furospinosulin-1, Marine Spongean Furanosesterterpene, Suppresses the Growth of Hypoxia-Adapted Cancer Cells by Binding to Transcriptional Regulators p54(nrb) and LEDGF/p75. Chembiochem : a European journal of chemical biology 10 26561285
2013 Melanoma inhibitory activity promotes melanoma development through activation of YBX1. Pigment cell & melanoma research 10 23672612
2016 Knockdown of p54nrb inhibits migration, invasion and TNF-α release of human acute monocytic leukemia THP1 cells. Oncology reports 9 27108701
2015 CARMing down the SINEs of anarchy: two paths to freedom from paraspeckle detention. Genes & development 9 25838539
2014 Decreased expression of P54(nrb) /NonO correlates with collagen deposition and fibrosis in human aortic dissection. Histopathology 9 24720418
2008 Microtubule interfering agents and KSP inhibitors induce the phosphorylation of the nuclear protein p54(nrb), an event linked to G2/M arrest. Journal of proteomics 9 18832053
2016 Tyrosine Residues Regulate Multiple Nuclear Functions of P54nrb. Journal of cellular physiology 8 27430900
2012 Paraspeckles: possible nuclear hubs by the RNA for the RNA. Biomolecular concepts 8 25436547
2022 The caspase-2 substrate p54nrb exhibits a multifaceted role in tumor cell death susceptibility via gene regulatory functions. Cell death & disease 6 35444189
2013 Nuclear actin polymerization from faster growing ends in the initial activation of Hox gene transcription are nuclear speckles involved? Transcription 6 24406343
2024 Nono induces Gadd45b to mediate DNA repair. Life science alliance 4 38843934
2015 Dynamical analysis of mCAT2 gene models with CTN-RNA nuclear retention. Physical biology 4 25619276
2025 Intranuclear paraspeckle-circular RNA TACC3 assembly forms RNA-DNA hybrids to facilitate MASH-related hepatocellular carcinoma growth in an m6A-dependent manner. Cancer communications (London, England) 3 41103024
2025 The metabolic enzyme GYS1 condenses with NONO/p54nrb in the nucleus and spatiotemporally regulates glycogenesis and myogenic differentiation. Cell death and differentiation 2 40200092
2022 Reversible acetylation modulates p54nrb/NONO-mediated expression of the interleukin 8 gene. Biochemical and biophysical research communications 2 35843094

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