Affinage

CKAP2

Cytoskeleton-associated protein 2 · UniProt Q8WWK9

Length
683 aa
Mass
77.0 kDa
Annotated
2026-06-09
51 papers in source corpus 19 papers cited in narrative 20 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CKAP2 is a mitotic spindle-associated microtubule-associated protein that acts as a potent catalyst of microtubule nucleation and growth, functioning as a central effector of bipolar spindle assembly and faithful chromosome segregation (PMID:35029146, PMID:38381793). In reconstituted systems CKAP2 lowers the critical tubulin concentration ~100-fold, increases the apparent growth-rate constant ~50-fold, and suppresses catastrophes, with a 284-residue intrinsically disordered domain sufficient to catalyze both microtubule polymerization and depolymerization without high-affinity tubulin binding [PMID:35029146, PMID:bio_10.1101_2025.09.02.673730]. CKAP2 co-localizes with microtubules across the cell cycle, concentrating on centrosomes after their separation in late G2 and on spindle poles and spindle microtubules from prophase through anaphase before relocating to chromatin and being cleared at mitotic exit (PMID:15504249, PMID:17376772, PMID:17339342, PMID:38381793). Loss of CKAP2 reduces cellular microtubule growth rates and produces spindle pole and centrosome defects, merotelic attachments, chromatin bridges, chromosome segregation errors, and aneuploidy (PMID:38381793, PMID:23737987, PMID:19158495, PMID:27055594). CKAP2 abundance is gated through the cell cycle by transcriptional and degradative controls: Cyclin A/CDK2-driven Sp1 phosphorylation at a promoter GC box drives a G2/M expression peak (PMID:22465120), while APC/C–Cdh1 ubiquitinates CKAP2 via an N-terminal KEN-box to enforce its destruction during mitotic exit, a step required for spindle bipolarity and cytokinesis completion (PMID:17376772, PMID:17339342). Mitotic kinases further tune CKAP2: Aurora kinase A, recruited with TPX2, directly phosphorylates CKAP2 to lower its microtubule affinity [PMID:bio_10.1101_2025.09.09.675182], and CDK1/cyclin B1 phosphorylation at T603/S608 is required for centrosome integrity and bipolar spindle formation (PMID:28706298). Overexpression or expression of non-degradable CKAP2 causes microtubule bundling, monopolar spindles, and aneuploidy (PMID:17376772, PMID:17339342), and CKAP2 also engages a p53-dependent G1 tetraploidy checkpoint and the Rb/p27 proliferative axis (PMID:16061649, PMID:16876122).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 2004 Medium

    Established CKAP2 as a bona fide microtubule-associated protein, answering whether it physically engages and influences microtubules.

    Evidence Immunocytochemical co-localization with tubulin and nocodazole-resistance assay in mouse cells

    PMID:15504249

    Open questions at the time
    • Did not distinguish direct stabilization from indirect effects
    • No biochemical reconstitution
    • Localization dynamics across mitosis not resolved
  2. 2006 Medium

    Linked CKAP2 to cell cycle progression by placing it upstream of the Rb/p27 pathway, framing it as more than a passive cytoskeletal component.

    Evidence siRNA knockdown and constitutive overexpression with pRB/p27 Western blots and proliferation assays in fibroblasts

    PMID:16876122

    Open questions at the time
    • Mechanism connecting CKAP2 to Rb phosphorylation unresolved
    • Correlative rather than direct molecular link
  3. 2007 High

    Defined how CKAP2 levels are restricted to mitosis, identifying it as an APC/C–Cdh1 substrate degraded via a KEN-box, and showed why this destruction matters for spindle bipolarity and cytokinesis.

    Evidence In vitro ubiquitination, KEN-box mutagenesis, Cdh1 manipulation, and non-degradable mutant phenotyping, plus cell-cycle localization imaging

    PMID:17339342 PMID:17376772

    Open questions at the time
    • Phosphorylation inputs controlling degradation timing not addressed
    • Did not establish the catalytic basis of microtubule effects
  4. 2007 Medium

    Connected CKAP2 to genome-integrity surveillance, showing its overexpression triggers tetraploidy in p53-null cells but a p53-mediated checkpoint in p53-competent cells.

    Evidence Overexpression in p53-null vs competent cells, promoter reporter, microarray, flow cytometry

    PMID:16061649

    Open questions at the time
    • Direct biochemical link between CKAP2 and p53 activation unclear
    • Whether checkpoint engagement is centrosome-driven not resolved
  5. 2008 Medium

    Revealed dynamic mitotic phosphoregulation of CKAP2 by identifying transient T596 phosphorylation in early mitosis that reverses at anaphase.

    Evidence Epitope mapping with monoclonal antibodies and immunofluorescence cell-cycle staging

    PMID:18779650

    Open questions at the time
    • Responsible kinase not identified
    • Functional consequence of T596 phosphorylation not tested
  6. 2009 Medium

    Demonstrated that CKAP2 is required for accurate chromosome segregation and spindle checkpoint function, defining a loss-of-function genomic instability phenotype.

    Evidence siRNA depletion with time-lapse microscopy, nuclear lamina imaging, and chromosomal instability assays

    PMID:19158495

    Open questions at the time
    • Molecular cause of checkpoint weakening not defined
    • Single-lab knockdown without rescue
  7. 2010 Medium

    Identified Aurora B as a direct CKAP2 kinase phosphorylating Ser627 within an RRSRRL motif that controls CKAP2 relocalization during late mitosis.

    Evidence In vitro Aurora B kinase assay, phosphomotif mutagenesis, and subcellular localization imaging

    PMID:20458174

    Open questions at the time
    • In vivo requirement of Ser627 for segregation fidelity not established
    • Relationship to other mitotic phosphosites unclear
  8. 2012 Medium

    Explained the G2/M transcriptional peak of CKAP2 through Cyclin A/CDK2-driven Sp1 phosphorylation at a promoter GC box, plus a CHR element.

    Evidence Promoter reporter, EMSA supershift, ChIP, and Cyclin A depletion/add-back

    PMID:22465120

    Open questions at the time
    • Direct phosphorylation of Sp1 inferred rather than mapped
    • CHR-binding repressor not identified
  9. 2013 Medium

    Showed CKAP2 is needed to focus microtubule minus ends to spindle poles, with depletion causing cage-like microtubule dispersal, merotely, and polyploidy.

    Evidence RNAi with cold-induced microtubule regrowth assay, immunofluorescence, and FISH

    PMID:23737987

    Open questions at the time
    • Pole-focusing partners not identified
    • Mechanistic link to minus-end clustering machinery unknown
  10. 2016 Medium

    Established CKAP2 as required for spindle bipolarity in diploid somatic cells and for tissue regeneration in vivo, extending its role beyond cancer cell lines.

    Evidence siRNA knockdown in primary hepatocytes with centrosome/spindle imaging and an in vivo partial hepatectomy model

    PMID:27055594

    Open questions at the time
    • Molecular basis of centrosome over-duplication not defined
    • In vivo phenotype not linked to a specific phosphosite
  11. 2017 Medium

    Defined a required CDK1/cyclin B1 phosphorylation of CKAP2 at T603/S608 for centrosome integrity, distinguishing it from Aurora B regulation.

    Evidence In vitro CDK1/cyclin B1 kinase assay with phosphosite mutant rescue in CKAP2-depleted hepatocytes

    PMID:28706298

    Open questions at the time
    • How these phosphosites alter microtubule activity not resolved
    • Interplay with Aurora and APC/C regulation untested
  12. 2017 Medium

    Implicated CKAP2 in oncogenic FAK–ERK2 signaling driving proliferation and motility in cervical carcinoma.

    Evidence Knockdown, pharmacological FAK/ERK2 inhibition, p-ERK2 Western blot, motility assays, and xenograft

    PMID:28522860

    Open questions at the time
    • Direct molecular link between CKAP2 and FAK activation unclear
    • May reflect proliferation phenotype rather than dedicated signaling role
  13. 2022 High

    Quantitatively redefined CKAP2 as the most potent known microtubule growth factor through direct in vitro reconstitution.

    Evidence In vitro microtubule polymerization assays with quantitative kinetic measurements

    PMID:35029146

    Open questions at the time
    • Domain responsible not yet mapped at this stage
    • Cellular requirement not yet tested in this study
  14. 2023 Low

    Added a post-transcriptional control layer via a ZC3H13–m6A–CKAP2 axis stabilizing CKAP2 mRNA in cervical cancer.

    Evidence m6A measurement, ZC3H13 manipulation, and CKAP2 rescue in cancer cells

    PMID:37943149

    Open questions at the time
    • Mechanistic detail limited; single lab
    • Direct m6A site on CKAP2 mRNA not mapped
    • Reader/effector linking m6A to stability not defined
  15. 2024 High

    Established the cellular requirement for CKAP2 in mitotic microtubule growth with rescue, confirming the in vitro activity operates in cells and tracking endogenous localization.

    Evidence CRISPR-Cas9 KO with live-cell plus-end tracking, endogenous tagging, and ectopic re-expression rescue

    PMID:38381793

    Open questions at the time
    • Did not resolve the molecular catalytic mechanism
    • Phospho-dependence of cellular activity not dissected here
  16. 2024 Low

    Placed CKAP2 downstream of TFDP1 transcriptional control in colorectal cancer proliferation.

    Evidence Co-IP, dual-luciferase reporter, and rescue experiments

    PMID:39403723

    Open questions at the time
    • Single Co-IP without reciprocal validation
    • Direct vs indirect transcriptional regulation unclear
  17. 2025 Medium

    Localized the catalytic activity to a 284-residue intrinsically disordered domain and proposed a transition-state-based mechanism distinct from canonical polymerases.

    Evidence In vitro reconstitution with isolated CKAP2 domain measuring polymerization/depolymerization (preprint)

    PMID:bio_10.1101_2025.09.02.673730

    Open questions at the time
    • Preprint, single lab, not peer reviewed
    • Structural basis of transition-state catalysis not defined
    • Domain behavior in cells not validated
  18. 2025 High

    Identified Aurora kinase A/TPX2 as specific CKAP2 interactors and showed Aurora A phosphorylation lowers CKAP2 microtubule affinity, defining a spindle-growth regulatory pathway.

    Evidence Endogenous Co-IP/MS, in vitro kinase assay, and cellular microtubule affinity measurement (preprint)

    PMID:bio_10.1101_2025.09.09.675182

    Open questions at the time
    • Preprint, not yet peer reviewed
    • Phosphosite(s) targeted by Aurora A not pinpointed in this entry
    • Integration with CDK1 and Aurora B inputs unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • The structural and transition-state basis by which the CKAP2 intrinsically disordered domain catalyzes microtubule growth without high-affinity tubulin binding, and how the multiple kinase inputs (Aurora A, Aurora B, CDK1) are integrated to time spindle assembly, remain unresolved.
  • No structural model of the catalytic IDR
  • No unified scheme integrating phospho-inputs with degradation timing
  • Direct binding/regulatory partners at spindle poles undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 4 GO:0098772 molecular function regulator activity 2
Localization
GO:0005815 microtubule organizing center 3 GO:0005856 cytoskeleton 3 GO:0005634 nucleus 2
Pathway
R-HSA-1640170 Cell Cycle 5
Partners
AURKAAURKBCDK1TFDP1TPX2

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2022 In vitro reconstitution assays showed that CKAP2 potently promotes microtubule nucleation, lowering the critical tubulin concentration ~100-fold, increases the apparent rate constant of microtubule growth ~50-fold, increases microtubule growth rates, and strongly suppresses catastrophes, identifying CKAP2 as the most potent microtubule growth factor known. In vitro reconstitution assay (microtubule polymerization) eLife High 35029146
2024 CRISPR-Cas9 knock-out and live-cell microtubule plus-end tracking showed that CKAP2 is required for normal microtubule growth rates in cells during mitosis; CKAP2 KO cells display reduced microtubule growth rates, increased chromosome segregation errors, and aneuploidy, all rescued by ectopic CKAP2 expression. Endogenously labeled CKAP2 localizes to the spindle during mitosis and shifts to chromatin upon mitotic exit before degradation. CRISPR-Cas9 KO, live-cell microtubule plus-end tracking imaging, ectopic re-expression rescue Proceedings of the National Academy of Sciences of the United States of America High 38381793
2025 Endogenous CKAP2 immunoprecipitation from mitotic cells followed by mass spectrometry identified Aurora kinase A and its activator TPX2 (but not Aurora B) as specific CKAP2 interactors. Aurora A directly phosphorylates CKAP2 in vitro and in cells, and this phosphorylation decreases CKAP2's microtubule affinity in cells and in vitro, revealing a regulatory pathway controlling spindle growth. Endogenous co-immunoprecipitation, mass spectrometry, in vitro kinase assay, microtubule affinity assay in cells bioRxivpreprint High bio_10.1101_2025.09.09.675182
2025 A 284-amino acid intrinsically disordered domain of CKAP2 is sufficient to catalyze both microtubule polymerization and depolymerization in vitro. CKAP2 promotes tubulin incorporation without high-affinity tubulin binding, suggesting a transition-state-based catalytic mechanism distinct from known microtubule polymerases. In vitro reconstitution with isolated CKAP2 domain, microtubule polymerization/depolymerization assays bioRxivpreprint Medium bio_10.1101_2025.09.02.673730
2007 CKAP2 is a physiological substrate of the APC/C–Cdh1 ubiquitin ligase. Ubiquitination and degradation of CKAP2 in vitro require a KEN-box motif and are mediated by Cdh1. CKAP2 protein levels are high in mitosis and drop during mitotic exit; Cdh1 overexpression reduces CKAP2 in a KEN-box-dependent manner and Cdh1 knockdown extends CKAP2 half-life. Ectopic expression of wild-type or non-degradable CKAP2 causes mitotic arrest with monopolar spindles and bundled microtubules. In vitro ubiquitination assay, KEN-box mutagenesis, Cdh1 overexpression/knockdown, cell cycle fractionation, immunofluorescence The Journal of biological chemistry High 17376772
2007 APC/C–Cdh1-mediated degradation of CKAP2 during mitotic exit is required for completion of cytokinesis and maintenance of spindle bipolarity. The KEN-box near the N-terminus is necessary for CKAP2 destruction. Overexpression of CKAP2 induces microtubule bundling, centrosome separation defects, and monopolar spindle formation; non-degradable CKAP2 significantly increases spindle defects and cytokinesis failure. KEN-box mutagenesis, non-degradable mutant expression, siRNA knockdown, immunofluorescence, live-cell imaging Molecular and cellular biology High 17339342
2004 Mouse CKAP2 protein co-localizes with microtubules throughout the cell cycle and overexpression stabilizes microtubules against nocodazole-induced depolymerization, establishing CKAP2 as a microtubule-associated protein with microtubule-stabilizing properties. Immunocytochemistry, nocodazole resistance assay, co-localization with tubulin Cancer science Medium 15504249
2005 Overexpressed CKAP2 co-localizes with and stabilizes microtubules. In p53-null cells, CKAP2 overexpression induces tetraploidy with aberrant centrosome numbers; in p53-competent cells it activates p53-mediated cell cycle arrest and apoptosis, suggesting CKAP2 activates the G1 tetraploidy checkpoint. DNA damage induces CKAP2 expression in a p53-dependent manner and p53 activates the Ckap2 promoter. DNA microarray, promoter reporter assay, overexpression in p53-null vs. p53-competent cells, immunofluorescence, flow cytometry Cancer research Medium 16061649
2009 siRNA-mediated depletion of CKAP2 in mammalian cells causes chromatin bridge formation, abnormal nuclear morphology (with abnormal nuclear lamina organization), defects in chromosome segregation revealed by time-lapse microscopy, reduced spindle checkpoint activity, reduced cell viability, and increased chromosomal instability. siRNA knockdown, time-lapse video microscopy, immunofluorescence of nuclear lamina, chromosomal instability assay Cell cycle (Georgetown, Tex.) Medium 19158495
2013 RNAi depletion of CKAP2 results in multipolar mitoses and spindle pole defects; CKAP2-depleted cells show dispersal of newly polymerized microtubules into a cage-like structure around chromatin immediately after mitotic release, indicating defective focusing of microtubule minus ends to spindle poles. CKAP2 suppression also increases merotelic kinetochore attachments, anaphase lagging, and polyploidy. RNAi knockdown, microtubule regrowth assay after cold treatment, immunofluorescence, FISH for aneuploidy PloS one Medium 23737987
2008 CKAP2 is transiently phosphorylated at Thr-596 (mouse T596, equivalent human site) specifically during early mitotic phases (prophase through metaphase); phosphorylation at T596 abolishes recognition by a monoclonal antibody (D-12-3) and was identified by epitope mapping. Immunofluorescence showed the phosphorylated form disappears at anaphase, indicating dephosphorylation during late mitosis. Epitope mapping with monoclonal antibodies, immunofluorescence on mitotic cells, phospho-peptide competition Experimental & molecular medicine Medium 18779650
2010 Aurora B kinase directly phosphorylates CKAP2 (TMAP) at Ser627 in vitro and in vivo. The motif (625)RRSRRL(630) surrounding Ser627 is required for efficient phosphorylation; substitutions within this motif both diminish kinase-substrate recognition and alter CKAP2 subcellular localization during late mitosis (mutants remain associated with microtubules/centrosomes instead of relocating to the chromosome region). In vitro kinase assay with Aurora B, site-directed mutagenesis of phosphorylation motif, immunofluorescence for subcellular localization Cell cycle (Georgetown, Tex.) Medium 20458174
2017 CDK1-cyclin B1 phosphorylates mouse CKAP2 at T603 and possibly S608 during mitosis. Ectopic expression of T603A and S608A mutants fails to rescue centrosomal abnormalities (amplified centrosomes and aberrant chromosome segregation) in CKAP2-depleted primary hepatocytes, demonstrating that these phosphorylation events are required for centrosome integrity and bipolar spindle formation. In vitro CDK1/cyclin B1 kinase assay, phosphorylation site mutagenesis, rescue experiment in CKAP2-depleted hepatocytes, immunofluorescence Experimental & molecular medicine Medium 28706298
2012 The human CKAP2 promoter contains a GC box (−41 to −32 bp) bound by transcription factor Sp1 (confirmed by supershift and ChIP assays) and a CHR element (−110 to −104 bp) that together drive cell-cycle-dependent CKAP2 transcription. Cyclin A/CDK2 promotes Sp1 binding to the GC box; depletion of Cyclin A reduces Sp1 occupancy, which is restored by adding back the Cyclin A/CDK2 complex, establishing Cyclin A-mediated Sp1 phosphorylation as the mechanism for G2/M-peak CKAP2 expression. Promoter reporter assay, EMSA supershift, ChIP assay, Cyclin A depletion with add-back experiment, mutagenesis of regulatory elements Biochemical and biophysical research communications Medium 22465120
2016 CKAP2-depleted primary hepatocytes display over-duplicated centrosomes, disjoined chromosomes from the metaphase plate, and proceed to apoptosis or multipolar cell division; CKAP2 is required for spindle bipolarity in diploid somatic cells. In vivo, CKAP2 depletion in mouse liver markedly reduces hepatic regeneration efficiency. siRNA knockdown in primary hepatocytes, immunofluorescence, in vivo partial hepatectomy model Biochemical and biophysical research communications Medium 27055594
2006 CKAP2 expression is cell-cycle-dependent in human foreskin fibroblasts, absent in G0/G1-arrested cells and peaking at G2/M. Knockdown of CKAP2 reduces pRB phosphorylation and increases p27 expression, reducing proliferation; constitutive CKAP2 expression increases pRB phosphorylation and enhances proliferation, placing CKAP2 upstream of the Rb/p27 pathway in cell cycle progression. siRNA knockdown, constitutive overexpression, Western blot for pRB/p27, proliferation assays Biochemical and biophysical research communications Medium 16876122
2007 CKAP2 localizes to centrosomal microtubules in late G2 (only after centrosome separation), then to spindle poles and spindle microtubules from prophase through anaphase, and disappears from microtubules during cytokinesis, as determined by live and fixed-cell immunofluorescence. Immunofluorescence across cell cycle stages, centrosome co-localization The Journal of biological chemistry Medium 17339342 17376772
2017 In cervical carcinoma cells, CKAP2 knockdown reduces phosphorylation of ERK2 (p-ERK2) and inhibits cell proliferation, migration, and invasion; FAK inhibitor (PF-562271) and ERK2 inhibitor (VX-11e) block CKAP2 overexpression-induced proliferation and motility, placing CKAP2 upstream of the FAK–ERK2 signaling axis. siRNA knockdown, pharmacological inhibitors, Western blot for p-ERK2, proliferation/migration/invasion assays, in vivo xenograft Scientific reports Medium 28522860
2023 ZC3H13 mediates N6-methyladenosine (m6A) modification of CKAP2 mRNA to stabilize its expression in cervical cancer cells; ZC3H13 inhibition decreases CKAP2 levels, and CKAP2 overexpression partially rescues the malignant phenotype suppressed by ZC3H13 knockdown, establishing a ZC3H13–m6A–CKAP2 regulatory axis. m6A modification level measurement, ZC3H13 overexpression/knockdown, CKAP2 rescue experiment, qRT-PCR, Western blot, proliferation/invasion/migration assays Critical reviews in immunology Low 37943149
2024 CKAP2 interacts with TFDP1 (confirmed by immunoprecipitation and dual-luciferase reporter assay), and TFDP1 positively regulates CKAP2 transcription; CKAP2 overexpression reverses the inhibitory effects of TFDP1 knockdown on colorectal cancer cell proliferation, migration, and invasion, placing CKAP2 downstream of TFDP1. Co-immunoprecipitation, dual-luciferase reporter assay, siRNA/overexpression rescue experiment Journal of microbiology and biotechnology Low 39403723

Source papers

Stage 0 corpus · 51 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 RSK promotes prostate cancer progression in bone through ING3, CKAP2, and PTK6-mediated cell survival. Molecular cancer research : MCR 46 25189355
2005 Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner. Cancer research 43 16061649
2007 CKAP2 is a spindle-associated protein degraded by APC/C-Cdh1 during mitotic exit. The Journal of biological chemistry 41 17376772
2007 Functional importance of the anaphase-promoting complex-Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis. Molecular and cellular biology 34 17339342
2004 Identification of a mouse cytoskeleton-associated protein, CKAP2, with microtubule-stabilizing properties. Cancer science 34 15504249
1990 A human glioma cell line retaining expression of GFAP and gangliosides, recognized by A2B5 and LB1 antibodies, after prolonged passage. Neuropathology and applied neurobiology 30 1982895
2021 LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis. Bioengineered 28 34596006
2009 TMAP/CKAP2 is essential for proper chromosome segregation. Cell cycle (Georgetown, Tex.) 28 19158495
2017 Involvement of FAK-ERK2 signaling pathway in CKAP2-induced proliferation and motility in cervical carcinoma cell lines. Scientific reports 27 28522860
2022 A chalcone-syringaldehyde hybrid inhibits triple-negative breast cancer cell proliferation and migration by inhibiting CKAP2-mediated FAK and STAT3 phosphorylation. Phytomedicine : international journal of phytotherapy and phytopharmacology 24 35429924
2006 A cytoskeleton-associated protein, TMAP/CKAP2, is involved in the proliferation of human foreskin fibroblasts. Biochemical and biophysical research communications 24 16876122
2017 Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in the TaAGP.L-B1 Gene. Frontiers in plant science 23 28848598
2015 LB-1 Exerts Antitumor Activity in Pancreatic Cancer by Inhibiting HIF-1α and Stat3 Signaling. Journal of cellular physiology 23 25655308
2021 Lactobacillus rhamnosus LB1 Alleviates Enterotoxigenic Escherichia coli-Induced Adverse Effects in Piglets by Improving Host Immune Response and Anti-Oxidation Stress and Restoring Intestinal Integrity. Frontiers in cellular and infection microbiology 22 34796123
2008 Transient phosphorylation of tumor associated microtubule associated protein (TMAP)/cytoskeleton associated protein 2 (CKAP2) at Thr-596 during early phases of mitosis. Experimental & molecular medicine 22 18779650
2017 CKAP2 Promotes Ovarian Cancer Proliferation and Tumorigenesis Through the FAK-ERK Pathway. DNA and cell biology 20 28933561
2022 Long non-coding RNA DLEU1 promotes malignancy of breast cancer by acting as an indispensable coactivator for HIF-1α-induced transcription of CKAP2. Cell death & disease 19 35853854
2007 Interaction of an Fe derivative of TMAP (Fe(TMAP)OAc) with DNA in comparison with free-base TMAP. International journal of biological macromolecules 17 17343908
2022 The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules. eLife 16 35029146
2013 CKAP2 ensures chromosomal stability by maintaining the integrity of microtubule nucleation sites. PloS one 16 23737987
2006 Tumor microvascular architecture phenotype (T-MAP) as a new concept for studies of angiogenesis and oncology. Journal of neuro-oncology 16 16708278
2023 ZC3H13 Enhances the Malignancy of Cervical Cancer by Regulating m6A Modification of CKAP2. Critical reviews in immunology 14 37943149
2024 Effect of Probiotic Lacticaseibacillus rhamnosus LB1.5 on Anxiety-like Behavior, Neuroprotection and Neuroinflammation Markers of Male Mice Fed a High-Fat Diet. Nutrients 11 38542789
2022 Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer. Cancers 11 35954424
2017 CKAP2 phosphorylation by CDK1/cyclinB1 is crucial for maintaining centrosome integrity. Experimental & molecular medicine 10 28706298
2010 Specific primary sequence requirements for Aurora B kinase-mediated phosphorylation and subcellular localization of TMAP during mitosis. Cell cycle (Georgetown, Tex.) 10 20458174
2019 Production, purification and characterization of a milk clotting enzyme from Bacillus methanolicus LB-1. Food science and biotechnology 8 31275710
2012 Cyclin A regulates a cell-cycle-dependent expression of CKAP2 through phosphorylation of Sp1. Biochemical and biophysical research communications 8 22465120
2011 Condensed chromatin staining of CKAP2 as surrogate marker for mitotic figures. Journal of cancer research and clinical oncology 8 22020800
1981 Effect of seminal plasma on Chlamydia trachomatis strain LB-1 in cell culture. Infection and immunity 8 6163729
2023 CKAP2 promotes cervical cancer progression by modulating the tumor microenvironment via NF-κB signaling. American journal of cancer research 7 37424820
2024 The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregation. Proceedings of the National Academy of Sciences of the United States of America 6 38381793
2021 Cytoskeleton-associated protein 2 (CKAP2) is regulated by vascular endothelial growth factor and p53 in retinal capillary endothelial cells under high-glucose conditions. Molecular and cellular endocrinology 6 34216644
2014 Comparative study of the interaction of meso-tetrakis (N-para-trimethyl-anilium) porphyrin (TMAP) in its free base and Fe derivative form with oligo(dA.dT)15 and oligo(dG.dC)15. Journal of biomolecular structure & dynamics 5 25273340
2013 RHOA, SEMA3B, and CKAP2 expression in leukaemia of different types: the results of a pilot experiment. Folia biologica 5 24280143
2019 Exploring the Folding Mechanism of Small Proteins GB1 and LB1. Journal of chemical theory and computation 4 31071262
2016 CKAP2 is necessary to ensure the faithful spindle bipolarity in a dividing diploid hepatocyte. Biochemical and biophysical research communications 4 27055594
2003 Tissue Molecular Anatomy Project (TMAP): an expression database for comparative cancer proteomics. Proteomics 4 12923770
1998 Alpha 2,3-specific desialylation of human red cells: effect on the autoantigens of the Pr, Sa and Sia-l1, -b1, -lb1 series. Vox sanguinis 4 9501410
2025 Identification and validation of CKAP2 as a novel biomarker in the development and progression of rheumatoid arthritis. Frontiers in immunology 2 40636121
2024 Analysis of the Probiotic Potential of Lactiplantibacillus plantarum LB1_P46 Isolated from the Mexican Fermented Pulque Beverage: A Functional and Genomic Analysis. Microorganisms 2 39203494
2024 CKAP2 Regulated by TFDP1 Promotes Metastasis and Proliferation of Colorectal Cancer through Affecting the Tumor Microenvironment. Journal of microbiology and biotechnology 2 39403723
2022 Biosynthesis of lactosucrose by a new source of β-fructofuranosidase from Bacillus methanolicus LB-1. Journal of bioscience and bioengineering 2 36564253
2001 [Evolutionarily-conserved gene CKAP2,located in region 13q14.3 of the human genome, is frequently rearranged in various tumors]. Genetika 2 11234418
2025 Identification of CKAP2 as a Potential Target for Prevention of Gastric Cancer Progression: A Multi-Omics Study. International journal of molecular sciences 1 40004022
2025 Effects of Lacticaseibacillus rhamnosus LB1.5 as potential probiotic supplement on the liver and adipose tissue of adult male mice to a high-fat diet. Molecular and cellular biochemistry 1 40581697
2024 Antifungal metabolites of biocontrol stain LB-1 and their inhibition mechanism against Botrytis cinerea. Frontiers in microbiology 1 39296286
2018 Complete Genome Sequence of Lactobacillus plantarum subsp. plantarum Strain LB1-2, Isolated from the Hindgut of European Honeybees, Apis mellifera L., from the Philippines. Genome announcements 1 29622612
2002 [The structure of the human oncogenesis-associated CKAP2 (LB1) gene]. Molekuliarnaia biologiia 1 12500535
2026 Antifungal activity of the culture filtrate of Chaetomium subaffine LB-1 against Bipolaris maydis and its underlying interaction mechanism. Frontiers in microbiology 0 42245497
2025 CKAP2, miR-941, miR-548 and LINC02577 as biomarkers for early diagnosis in colorectal cancer. Scientific reports 0 41476086

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