Affinage

CKAP2

Cytoskeleton-associated protein 2 · UniProt Q8WWK9

Length
683 aa
Mass
77.0 kDa
Annotated
2026-04-28
51 papers in source corpus 20 papers cited in narrative 20 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CKAP2 is a microtubule-associated protein that functions as a potent microtubule polymerase to promote spindle assembly, chromosome segregation fidelity, and centrosome integrity during mitosis. In vitro reconstitution demonstrates that CKAP2 lowers the critical tubulin concentration for microtubule nucleation ~100-fold, accelerates growth rates, and suppresses catastrophes through an intrinsically disordered catalytic domain, while CKAP2 knockout cells exhibit reduced microtubule growth rates, kinetochore-microtubule misattachments, chromosome segregation errors, and aneuploidy (PMID:35029146, PMID:38381793). CKAP2 protein levels are cell-cycle-controlled through transcriptional activation via Cyclin A/Cdk2-mediated Sp1 phosphorylation and proteasomal degradation by APC/C-Cdh1 through a KEN-box motif during mitotic exit, and its mitotic localization and microtubule affinity are regulated by Aurora B phosphorylation at Ser627 and CDK1/cyclin B1 phosphorylation at T603/S608 (PMID:22465120, PMID:17376772, PMID:20458174, PMID:28706298). Loss of CKAP2 causes multipolar spindles, centrosome over-duplication, reduced spindle checkpoint activity, and chromosomal instability, establishing it as essential for mitotic fidelity (PMID:19158495, PMID:23737987).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2004 Medium

    The initial question of whether CKAP2 interacts with the cytoskeleton was answered by demonstrating that it colocalizes with microtubules throughout the cell cycle and stabilizes them against nocodazole-induced depolymerization, establishing CKAP2 as a microtubule-associated protein.

    Evidence Immunocytochemistry and nocodazole resistance assay in mouse cells

    PMID:15504249

    Open questions at the time
    • Single lab observation without biochemical demonstration of direct microtubule binding
    • Mechanism of stabilization unknown
  2. 2005 High

    CKAP2 was connected to the DNA damage response when it was identified as a p53 transcriptional target whose overexpression causes tetraploidy in p53-null cells but triggers p53-dependent arrest in p53-competent cells, linking microtubule stabilization to genomic integrity surveillance.

    Evidence DNA microarray, promoter assay, overexpression in p53-null vs p53-competent cells

    PMID:16061649

    Open questions at the time
    • Whether p53 regulation of CKAP2 is relevant during normal cell division vs. stress only
    • Mechanism by which CKAP2 overexpression causes tetraploidy not defined
  3. 2007 High

    The question of how CKAP2 protein levels are cleared after mitosis was resolved by showing that APC/C-Cdh1 ubiquitinates CKAP2 through a KEN-box motif, and that failure to degrade CKAP2 causes monopolar spindles and cytokinesis failure, establishing cell-cycle-dependent proteolysis as essential for proper mitotic exit.

    Evidence In vitro ubiquitination assay, KEN-box mutagenesis, non-degradable mutant expression, time-lapse microscopy; replicated by two independent labs

    PMID:17339342 PMID:17376772

    Open questions at the time
    • Whether other E3 ligases contribute to CKAP2 turnover
    • How excess CKAP2 mechanistically generates monopolar spindles
  4. 2009 High

    Depletion studies revealed that CKAP2 is required not only for spindle architecture but also for spindle checkpoint function, as CKAP2 knockdown causes chromosome segregation defects and reduced checkpoint activity, broadening its role beyond microtubule stabilization.

    Evidence siRNA knockdown with time-lapse video microscopy, nuclear lamina immunofluorescence, spindle checkpoint assay

    PMID:19158495

    Open questions at the time
    • Mechanism by which CKAP2 influences the spindle assembly checkpoint is unknown
    • Whether checkpoint reduction is direct or secondary to kinetochore-microtubule defects
  5. 2010 High

    Aurora B was identified as a mitotic kinase that phosphorylates CKAP2 at Ser627 to control its subcellular relocalization from spindle microtubules to the chromosome region during late mitosis, providing the first mechanism for dynamic regulation of CKAP2 localization.

    Evidence In vitro kinase assay, site-directed mutagenesis, immunofluorescence localization across mitotic stages

    PMID:20458174

    Open questions at the time
    • Functional consequence of Ser627 phosphorylation on microtubule dynamics not measured
    • Whether Aurora B-mediated relocalization is required for chromosome segregation fidelity
  6. 2012 High

    The transcriptional mechanism driving cell-cycle-dependent CKAP2 expression was defined: Cyclin A/Cdk2 phosphorylates the transcription factor Sp1, which binds a GC box in the CKAP2 promoter to activate transcription, explaining how CKAP2 accumulates as cells approach mitosis.

    Evidence Promoter deletion/mutation analysis, ChIP, Cyclin A depletion and add-back with Sp1 binding assay

    PMID:22465120

    Open questions at the time
    • Whether additional transcription factors cooperate with Sp1
    • Post-transcriptional regulation at the mRNA level not fully explored at this point
  7. 2013 High

    CKAP2 was shown to maintain spindle pole integrity by focusing microtubule minus ends, as its depletion disperses newly nucleated microtubules, causes multipolar spindles, and increases merotelic kinetochore attachments leading to aneuploidy.

    Evidence RNAi, microtubule regrowth assay, immunofluorescence, chromosome segregation analysis

    PMID:23737987

    Open questions at the time
    • Direct mechanism of minus-end focusing by CKAP2 not defined
    • Whether CKAP2 cooperates with NuMA or other pole-focusing factors
  8. 2017 High

    CDK1/cyclin B1 was identified as a second mitotic kinase regulating CKAP2 via phosphorylation at T603/S608, with phospho-dead mutants failing to rescue centrosomal abnormalities in depleted cells, demonstrating that multiple kinase inputs converge on CKAP2 to maintain centrosome integrity.

    Evidence Phosphorylation site identification, site-directed mutagenesis, rescue experiments in CKAP2-depleted cells

    PMID:28706298

    Open questions at the time
    • How CDK1 and Aurora B phosphorylation are coordinated on CKAP2
    • Whether phosphorylation at T603/S608 affects microtubule binding affinity directly
  9. 2022 High

    In vitro reconstitution established that CKAP2 is the most potent microtubule growth factor known, lowering the nucleation critical concentration 100-fold, increasing the growth rate constant 50-fold, and suppressing catastrophes, transforming the understanding of CKAP2 from a passive stabilizer to an active polymerase.

    Evidence TIRF microscopy-based in vitro reconstitution with purified components and single-molecule dynamics measurements

    PMID:35029146

    Open questions at the time
    • Structural basis for how CKAP2 catalyzes tubulin addition unknown
    • Whether polymerase activity is regulated by mitotic phosphorylation in vitro
  10. 2024 High

    CRISPR knockout of endogenous CKAP2 quantitatively demonstrated reduced microtubule growth rates and increased chromosome segregation errors rescued by ectopic CKAP2 expression, validating the in vitro polymerase activity as the physiologically relevant mechanism for ensuring mitotic fidelity.

    Evidence CRISPR-Cas9 knock-in/knock-out, live cell microtubule plus-end tracking, chromosome segregation analysis with rescue

    PMID:38381793

    Open questions at the time
    • Relative contribution of CKAP2 polymerase vs. stabilizer activity in vivo not deconvolved
    • Whether CKAP2 loss triggers compensatory upregulation of other polymerases
  11. 2025 High

    Aurora Kinase A/TPX2 was identified as a third kinase complex regulating CKAP2, phosphorylating it to reduce microtubule affinity and limit spindle size, revealing a negative feedback mechanism that constrains CKAP2 polymerase activity during mitosis.

    Evidence (preprint) Endogenous co-IP/mass spectrometry, in vitro kinase assay, phospho-mimic mutagenesis, microtubule binding and live imaging

    PMID:bio_10.1101_2025.09.09.675182

    Open questions at the time
    • Not yet peer-reviewed
    • Specific phosphorylation sites on CKAP2 targeted by Aurora A not all mapped
    • How Aurora A and CDK1 phosphorylation events are temporally coordinated
  12. 2025 Medium

    A 284-amino acid intrinsically disordered domain was identified as sufficient for both microtubule polymerization and depolymerization catalysis without high-affinity tubulin binding, suggesting a transition-state-based catalytic mechanism distinct from classical polymerases like XMAP215.

    Evidence (preprint) In vitro reconstitution with isolated CKAP2 domain, polymerization/depolymerization and tubulin binding assays

    PMID:bio_10.1101_2025.09.02.673730

    Open questions at the time
    • Not yet peer-reviewed
    • Structural basis of the catalytic domain remains undefined
    • Whether the depolymerization activity is physiologically relevant in cells

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis for CKAP2's catalytic mechanism, how the three kinase inputs (Aurora B, CDK1, Aurora A) are temporally integrated to tune CKAP2 activity across mitotic stages, and whether CKAP2's spindle checkpoint role is direct or secondary to kinetochore-microtubule attachment defects.
  • No structural model of CKAP2 or its catalytic domain exists
  • Mechanism linking CKAP2 to spindle assembly checkpoint signaling undefined
  • Physiological relevance of CKAP2's depolymerase activity in cells untested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 5 GO:0140096 catalytic activity, acting on a protein 2
Localization
GO:0005856 cytoskeleton 4 GO:0005815 microtubule organizing center 3 GO:0005694 chromosome 1
Pathway
R-HSA-1640170 Cell Cycle 6 R-HSA-392499 Metabolism of proteins 2
Partners
AURKAAURKBCCNA2CCNB1CDH1CDK1SP1TPX2

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2004 Mouse CKAP2 (mCKAP2) colocalizes with microtubules throughout the cell cycle and overexpression stabilizes microtubules against nocodazole-induced depolymerization, identifying CKAP2 as a microtubule-associated protein with microtubule-stabilizing properties. Immunocytochemistry, colocalization with microtubule markers, nocodazole resistance assay Cancer science Medium 15504249
2005 CKAP2 is a p53 target gene; DNA damage induces CKAP2 expression in a p53-dependent manner, p53 activates the Ckap2 promoter, overexpressed CKAP2 colocalizes with and stabilizes microtubules, and in p53-null cells CKAP2 overexpression induces tetraploidy with aberrant centrosome numbers, while in p53-competent cells it activates p53-mediated cell cycle arrest and apoptosis. DNA microarray, promoter assay, immunofluorescence colocalization, overexpression in p53-null vs p53-competent cells Cancer research High 16061649
2006 CKAP2/TMAP is expressed in a cell-cycle-dependent manner (low in G0/G1, peaking at G2/M), and knockdown reduces pRB phosphorylation and increases p27 expression, reducing proliferation, while constitutive expression increases pRB phosphorylation and enhances proliferation. siRNA knockdown, cell cycle synchronization, Western blot for pRB and p27, proliferation assay Biochemical and biophysical research communications Medium 16876122
2007 CKAP2 is a substrate of APC/C-Cdh1 ubiquitin ligase; ubiquitination and degradation of CKAP2 in vitro requires a KEN-box motif and is mediated by Cdh1. CKAP2 levels fluctuate across the cell cycle (high in mitosis, low during mitotic exit); Cdh1 overexpression reduces CKAP2 in a KEN-box-dependent manner, and Cdh1 knockdown increases CKAP2 half-life. CKAP2 associates with centrosomal microtubules in late G2 and with spindle poles and spindle microtubules from prophase through anaphase. Overexpression causes mitotic arrest with monopolar spindles containing highly bundled microtubules. In vitro ubiquitination assay, KEN-box mutagenesis, Cdh1 overexpression/knockdown, immunofluorescence, cell cycle profiling The Journal of biological chemistry High 17376772
2007 APC/C-Cdh1-mediated degradation of CKAP2 during mitotic exit is required for proper cytokinesis and spindle bipolarity; the KEN box near the N-terminus is necessary for its destruction. Overexpression causes centrosome separation defects and monopolar spindle formation. A non-degradable CKAP2 mutant increases spindle defects and cytokinesis failure. KEN-box mutagenesis, non-degradable mutant expression, overexpression, time-lapse microscopy, immunofluorescence Molecular and cellular biology High 17339342
2008 CKAP2/TMAP is transiently phosphorylated at Thr-596 specifically during early phases of mitosis (prophase to metaphase); phosphorylation at T596 abolishes reactivity of monoclonal antibody D-12-3, and immunofluorescence shows loss of epitope from prophase to metaphase with recovery in anaphase. Epitope mapping, phospho-specific antibody reactivity, immunofluorescence across mitotic stages Experimental & molecular medicine Medium 18779650
2009 CKAP2/TMAP depletion by siRNA causes defects in chromosome segregation (chromatin bridges, abnormal nuclear morphology, abnormal nuclear lamina organization) without significant alteration of the spindle apparatus; spindle checkpoint activity is significantly reduced in CKAP2-depleted cells, leading to chromosomal instability and reduced cell viability. siRNA knockdown, time-lapse video microscopy, immunofluorescence for nuclear lamina, spindle checkpoint assay Cell cycle High 19158495
2010 Aurora B kinase phosphorylates CKAP2/TMAP at Ser627 both in vitro and in vivo; neighboring conserved residues are required for efficient phosphorylation. Mutations at the phosphorylation motif (RRSRRL) cause CKAP2 to remain associated with microtubules and centrosomes throughout mitosis instead of relocalizing to the chromosome region during late mitosis. In vitro kinase assay, in vivo phosphorylation, site-directed mutagenesis, immunofluorescence localization Cell cycle High 20458174
2012 Cell-cycle-dependent expression of CKAP2 is regulated at the transcriptional level by Cyclin A/Cdk2 phosphorylation of Sp1, which binds a GC box in the CKAP2 promoter (-41 to -32 bp). Mutation of the GC box abolishes CKAP2 promoter activity; Cyclin A depletion reduces Sp1 binding to the GC box, and adding back Cyclin A/Cdk2 complex restores it. Promoter deletion/mutation analysis, supershift assay, ChIP assay, Cyclin A depletion and add-back Biochemical and biophysical research communications High 22465120
2013 CKAP2 depletion by RNAi causes multipolar mitoses, spindle pole defects, and dispersal of newly polymerized microtubules through the chromatin region (cage-like structure) in early mitosis, indicating CKAP2 maintains microtubule nucleation site integrity by focusing microtubule minus ends to spindle poles. CKAP2 depletion also increases merotelic attachments, anaphase lagging, and polyploidy. RNAi, immunofluorescence, microtubule regrowth assay, chromosome segregation analysis PloS one High 23737987
2016 CKAP2 depletion in primary diploid hepatocytes leads to over-duplicated centrosomes, disjoined chromosomes from the metaphase plate, apoptosis or multipolar cell division, and markedly decreased liver regeneration efficiency in vivo, demonstrating a physiological role in spindle bipolarity and chromosomal stability. siRNA knockdown in primary hepatocytes, immunofluorescence, in vivo liver regeneration model Biochemical and biophysical research communications Medium 27055594
2017 CKAP2 phosphorylation at T603 (and possibly S608) by CDK1-cyclin B1 during mitosis is critical for centrosome integrity; ectopic expression of T603A and S608A mutants fails to restore centrosomal abnormalities in CKAP2-depleted cells. Phosphorylation site identification, site-directed mutagenesis, rescue experiments in CKAP2-depleted cells, centrosome analysis Experimental & molecular medicine High 28706298
2017 CKAP2 knockdown in cervical carcinoma cells inhibits FAK-ERK2 signaling (reduced p-ERK2), and FAK inhibitor and ERK2 inhibitor block CKAP2 overexpression-induced proliferation, migration, and invasion, placing CKAP2 upstream of FAK-ERK2 pathway. siRNA knockdown, FAK inhibitor (PF-562271), ERK2 inhibitor (VX-11e), Western blot for p-ERK2, in vivo tumor growth Scientific reports Medium 28522860
2022 In vitro reconstitution shows CKAP2 lowers the critical tubulin concentration for microtubule nucleation 100-fold, increases the apparent rate constant k of microtubule growth by 50-fold, increases microtubule growth rates, and strongly suppresses catastrophes, identifying CKAP2 as the most potent microtubule growth factor known. In vitro reconstitution assay, TIRF microscopy, single-molecule microtubule dynamics measurements eLife High 35029146
2022 DLEU1 lncRNA interacts with HIF-1α to collectively activate transcription of CKAP2; CKAP2 in turn activates ERK and STAT3 signaling to mediate DLEU1 pro-tumor activities in breast cancer. Co-IP for DLEU1-HIF-1α interaction, CKAP2 knockdown/overexpression, Western blot for ERK and STAT3 phosphorylation, xenograft model Cell death & disease Medium 35853854
2024 Endogenous CKAP2 (CRISPR knock-in) localizes to the mitotic spindle during mitosis and rapidly shifts to chromatin upon mitotic exit before degradation. CKAP2 KO cells display reduced microtubule growth rates, increased chromosome segregation errors, aneuploidy, and accumulation of kinetochore-microtubule misattachments; ectopic CKAP2 expression rescues microtubule growth rates and chromosome segregation fidelity. CRISPR-Cas9 knock-in/knock-out, live cell microtubule plus-end tracking imaging, chromosome segregation analysis Proceedings of the National Academy of Sciences of the United States of America High 38381793
2025 Aurora Kinase A, together with its cofactor TPX2, interacts with CKAP2 throughout mitosis (identified by co-IP/MS of endogenous CKAP2 from mitotic cells). Aurora Kinase A phosphorylates CKAP2 both in vitro and in mitotic cells; phosphorylated CKAP2 has reduced affinity for microtubules in vitro. Expression of Aurora A phospho-mimic CKAP2 mutants results in smaller mitotic spindles and reduced microtubule stability. Endogenous co-IP/mass spectrometry, in vitro kinase assay, phospho-mimic mutagenesis, microtubule binding assay, live cell imaging bioRxivpreprint High bio_10.1101_2025.09.09.675182
2025 A 284-amino acid intrinsically disordered domain of CKAP2 is sufficient to catalyze both microtubule polymerization and depolymerization in vitro. CKAP2 promotes tubulin incorporation without high-affinity tubulin binding, suggesting a transition-state-based catalytic mechanism distinct from known microtubule polymerases. In vitro reconstitution with isolated CKAP2 domain, microtubule polymerization/depolymerization assays, tubulin binding assays bioRxivpreprint Medium bio_10.1101_2025.09.02.673730
2023 ZC3H13 mediates N6-methyladenosine (m6A) modification of CKAP2 mRNA to enhance CKAP2 expression in cervical cancer cells; CKAP2 overexpression partially restores malignant phenotypes suppressed by ZC3H13 inhibition. m6A modification assay, ZC3H13 knockdown/overexpression, CKAP2 rescue experiment, qRT-PCR Critical reviews in immunology Medium 37943149
2021 DARS-AS1 lncRNA functions as a ceRNA to competitively sponge miR-3200-5p, thereby upregulating CKAP2 expression; CKAP2 activates the FAK-ERK pathway. miR-3200-5p directly targets CKAP2 and inactivates FAK-ERK signaling. Dual-luciferase reporter assay, miR-3200-5p mimic/inhibitor, CKAP2 knockdown/overexpression, Western blot for FAK-ERK Bioengineered Medium 34596006

Source papers

Stage 0 corpus · 51 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 RSK promotes prostate cancer progression in bone through ING3, CKAP2, and PTK6-mediated cell survival. Molecular cancer research : MCR 45 25189355
2005 Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner. Cancer research 43 16061649
2007 CKAP2 is a spindle-associated protein degraded by APC/C-Cdh1 during mitotic exit. The Journal of biological chemistry 41 17376772
2007 Functional importance of the anaphase-promoting complex-Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis. Molecular and cellular biology 34 17339342
2004 Identification of a mouse cytoskeleton-associated protein, CKAP2, with microtubule-stabilizing properties. Cancer science 34 15504249
1990 A human glioma cell line retaining expression of GFAP and gangliosides, recognized by A2B5 and LB1 antibodies, after prolonged passage. Neuropathology and applied neurobiology 30 1982895
2021 LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis. Bioengineered 28 34596006
2009 TMAP/CKAP2 is essential for proper chromosome segregation. Cell cycle (Georgetown, Tex.) 28 19158495
2017 Involvement of FAK-ERK2 signaling pathway in CKAP2-induced proliferation and motility in cervical carcinoma cell lines. Scientific reports 27 28522860
2022 A chalcone-syringaldehyde hybrid inhibits triple-negative breast cancer cell proliferation and migration by inhibiting CKAP2-mediated FAK and STAT3 phosphorylation. Phytomedicine : international journal of phytotherapy and phytopharmacology 24 35429924
2006 A cytoskeleton-associated protein, TMAP/CKAP2, is involved in the proliferation of human foreskin fibroblasts. Biochemical and biophysical research communications 24 16876122
2017 Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in the TaAGP.L-B1 Gene. Frontiers in plant science 23 28848598
2015 LB-1 Exerts Antitumor Activity in Pancreatic Cancer by Inhibiting HIF-1α and Stat3 Signaling. Journal of cellular physiology 22 25655308
2008 Transient phosphorylation of tumor associated microtubule associated protein (TMAP)/cytoskeleton associated protein 2 (CKAP2) at Thr-596 during early phases of mitosis. Experimental & molecular medicine 22 18779650
2021 Lactobacillus rhamnosus LB1 Alleviates Enterotoxigenic Escherichia coli-Induced Adverse Effects in Piglets by Improving Host Immune Response and Anti-Oxidation Stress and Restoring Intestinal Integrity. Frontiers in cellular and infection microbiology 20 34796123
2017 CKAP2 Promotes Ovarian Cancer Proliferation and Tumorigenesis Through the FAK-ERK Pathway. DNA and cell biology 19 28933561
2022 Long non-coding RNA DLEU1 promotes malignancy of breast cancer by acting as an indispensable coactivator for HIF-1α-induced transcription of CKAP2. Cell death & disease 17 35853854
2007 Interaction of an Fe derivative of TMAP (Fe(TMAP)OAc) with DNA in comparison with free-base TMAP. International journal of biological macromolecules 17 17343908
2022 The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules. eLife 16 35029146
2020 Behavioral Changes in Glutenin Macropolymer Fermented by Lactobacillus plantarum LB-1 to Promote the Rheological and Gas Production Properties of Dough. Journal of agricultural and food chemistry 16 32096634
2013 CKAP2 ensures chromosomal stability by maintaining the integrity of microtubule nucleation sites. PloS one 16 23737987
2006 Tumor microvascular architecture phenotype (T-MAP) as a new concept for studies of angiogenesis and oncology. Journal of neuro-oncology 16 16708278
2023 ZC3H13 Enhances the Malignancy of Cervical Cancer by Regulating m6A Modification of CKAP2. Critical reviews in immunology 12 37943149
2024 Effect of Probiotic Lacticaseibacillus rhamnosus LB1.5 on Anxiety-like Behavior, Neuroprotection and Neuroinflammation Markers of Male Mice Fed a High-Fat Diet. Nutrients 11 38542789
2022 Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer. Cancers 10 35954424
2017 CKAP2 phosphorylation by CDK1/cyclinB1 is crucial for maintaining centrosome integrity. Experimental & molecular medicine 10 28706298
2010 Specific primary sequence requirements for Aurora B kinase-mediated phosphorylation and subcellular localization of TMAP during mitosis. Cell cycle (Georgetown, Tex.) 10 20458174
2019 Production, purification and characterization of a milk clotting enzyme from Bacillus methanolicus LB-1. Food science and biotechnology 8 31275710
2012 Cyclin A regulates a cell-cycle-dependent expression of CKAP2 through phosphorylation of Sp1. Biochemical and biophysical research communications 8 22465120
2011 Condensed chromatin staining of CKAP2 as surrogate marker for mitotic figures. Journal of cancer research and clinical oncology 8 22020800
1981 Effect of seminal plasma on Chlamydia trachomatis strain LB-1 in cell culture. Infection and immunity 8 6163729
2024 The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregation. Proceedings of the National Academy of Sciences of the United States of America 6 38381793
2023 CKAP2 promotes cervical cancer progression by modulating the tumor microenvironment via NF-κB signaling. American journal of cancer research 6 37424820
2021 Cytoskeleton-associated protein 2 (CKAP2) is regulated by vascular endothelial growth factor and p53 in retinal capillary endothelial cells under high-glucose conditions. Molecular and cellular endocrinology 6 34216644
2014 Comparative study of the interaction of meso-tetrakis (N-para-trimethyl-anilium) porphyrin (TMAP) in its free base and Fe derivative form with oligo(dA.dT)15 and oligo(dG.dC)15. Journal of biomolecular structure & dynamics 5 25273340
2013 RHOA, SEMA3B, and CKAP2 expression in leukaemia of different types: the results of a pilot experiment. Folia biologica 5 24280143
2019 Exploring the Folding Mechanism of Small Proteins GB1 and LB1. Journal of chemical theory and computation 4 31071262
2016 CKAP2 is necessary to ensure the faithful spindle bipolarity in a dividing diploid hepatocyte. Biochemical and biophysical research communications 4 27055594
2003 Tissue Molecular Anatomy Project (TMAP): an expression database for comparative cancer proteomics. Proteomics 4 12923770
1998 Alpha 2,3-specific desialylation of human red cells: effect on the autoantigens of the Pr, Sa and Sia-l1, -b1, -lb1 series. Vox sanguinis 4 9501410
2025 Identification and validation of CKAP2 as a novel biomarker in the development and progression of rheumatoid arthritis. Frontiers in immunology 2 40636121
2024 CKAP2 Regulated by TFDP1 Promotes Metastasis and Proliferation of Colorectal Cancer through Affecting the Tumor Microenvironment. Journal of microbiology and biotechnology 2 39403723
2001 [Evolutionarily-conserved gene CKAP2,located in region 13q14.3 of the human genome, is frequently rearranged in various tumors]. Genetika 2 11234418
2025 Identification of CKAP2 as a Potential Target for Prevention of Gastric Cancer Progression: A Multi-Omics Study. International journal of molecular sciences 1 40004022
2025 Effects of Lacticaseibacillus rhamnosus LB1.5 as potential probiotic supplement on the liver and adipose tissue of adult male mice to a high-fat diet. Molecular and cellular biochemistry 1 40581697
2024 Analysis of the Probiotic Potential of Lactiplantibacillus plantarum LB1_P46 Isolated from the Mexican Fermented Pulque Beverage: A Functional and Genomic Analysis. Microorganisms 1 39203494
2024 Antifungal metabolites of biocontrol stain LB-1 and their inhibition mechanism against Botrytis cinerea. Frontiers in microbiology 1 39296286
2022 Biosynthesis of lactosucrose by a new source of β-fructofuranosidase from Bacillus methanolicus LB-1. Journal of bioscience and bioengineering 1 36564253
2018 Complete Genome Sequence of Lactobacillus plantarum subsp. plantarum Strain LB1-2, Isolated from the Hindgut of European Honeybees, Apis mellifera L., from the Philippines. Genome announcements 1 29622612
2002 [The structure of the human oncogenesis-associated CKAP2 (LB1) gene]. Molekuliarnaia biologiia 1 12500535
2025 CKAP2, miR-941, miR-548 and LINC02577 as biomarkers for early diagnosis in colorectal cancer. Scientific reports 0 41476086