Affinage

CDK1

Cyclin-dependent kinase 1 · UniProt P06493

Length
297 aa
Mass
34.1 kDa
Annotated
2026-06-09
100 papers in source corpus 66 papers cited in narrative 65 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CDK1 is the master mitotic kinase: it acquires protein kinase activity only upon binding a cyclin partner (cyclin B/MPF or cyclin A), and the active complex phosphorylates hundreds of substrates to drive entry into and progression through M phase (PMID:12045216, PMID:14593728). Its activity is gated by inhibitory T-loop and Y15/T14 phosphorylation imposed by Wee1/MYT1, removed by CDC25, and CDK1 feeds back onto its own inhibitor by both activating and then inactivating Wee1/Swe1, building the switch-like bistability of mitotic onset (PMID:16096060, PMID:15589158, PMID:23146904, PMID:30008317); CDK-activating kinase phosphorylation of the T-loop and the accessory subunit CKS1 are required for full activity and for processive multisite substrate phosphorylation, including at non-proline-directed sites (PMID:36840943, PMID:34791727). Once active, CDK1 orchestrates the structural reorganizations of mitosis—chromosome condensation through multisite phosphorylation of the condensin subunit Smc4 (PMID:25691469), sister-chromatid cohesion release via Sororin phosphorylation (PMID:23901111, PMID:21878504), nuclear envelope breakdown through importin-α1 and CHMP7 phosphorylation (PMID:34286694, PMID:31434716), and kinetochore-microtubule attachment and spindle checkpoint signaling by priming PLK1 docking sites on BUB1/CENP-U and CLASP2, phosphorylating MPS1 at S281, localizing to unattached kinetochores via MAD1, and tuning Dam1/Nsk1 attachments (PMID:33248027, PMID:30674582, PMID:30674583, PMID:23045552, PMID:32946748, PMID:22065639). CDK1 also sets the timing of mitotic exit: its inactivation is required for anaphase spindle dynamics, cytokinesis, APC/C-Cdh1 activation, and the RepoMan/PP2A phosphatase switches that reverse mitotic phosphorylation (PMID:9230080, PMID:26674376, PMID:32900880, PMID:10694434). Beyond the canonical cell-cycle program, CDK1 phosphorylates substrates that sustain cap-dependent and 5'TOP-mRNA translation (4E-BP1, LARP1) (PMID:25883264, PMID:32040547), suppress innate immune sensing of self-DNA during mitosis (cGAS) (PMID:32351706), control DNA-repair pathway choice and recombination intermediate resolution (WRN, Mus81-Mms4) (PMID:23531881, PMID:27634057), drive mitotic autophagy (ULK1-ATG13) (PMID:32516310), and boost mitochondrial bioenergetics and fission (complex I subunits, SIRT3, Drp1) (PMID:26141949, PMID:26670043). In cancer contexts CDK1 phosphorylation reprograms transcription factors and deubiquitinases (Sox2, TFCP2L1, USP9X, USP29) and directs E3-ligase-dependent degradation of multiple targets (kindlin, ACSL4, pVHL), and CDK1 itself is stabilized by deubiquitinases to support tumor proliferation (PMID:31709755, PMID:35469017, PMID:32152317, PMID:37428466, PMID:36782089, PMID:36813923, PMID:37667382).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2002 High

    Established that CDK1 is a cyclin-dependent enzyme—catalytically inert until it binds cyclin B—defining the MPF activity that triggers M phase entry.

    Evidence Biochemical purification and reconstitution of cyclin B-CDK1 (MPF) across starfish, invertebrate eggs, and vertebrate cells

    PMID:12045216

    Open questions at the time
    • Does not resolve which substrates drive specific mitotic events
    • Does not address cyclin A versus cyclin B specificity
  2. 2005 High

    Resolved how CDK1 controls its own activation threshold by showing it both activates and then inactivates its inhibitor Wee1/Swe1, creating bistable feedback.

    Evidence In vitro kinase assay, Co-IP, and phospho-site mutagenesis in budding yeast; complemented by Drosophila dwee1 LOF showing Y15 inhibitory phosphorylation times mitosis

    PMID:15589158 PMID:16096060

    Open questions at the time
    • Quantitative contribution of feedback to switch sharpness not defined
    • MYT1/T14 arm addressed in later work
  3. 2003 Medium

    Defined that cyclin B1 binding drives a conformational change enabling CDK1 activation, and that nuclear translocation of the active complex is required to reach nuclear substrates.

    Evidence Biochemical and nuclear import/export analysis (mechanistic review of replicated biochemistry)

    PMID:14593728

    Open questions at the time
    • Review synthesis rather than single primary dataset
    • Compartmentalized activity dynamics resolved only later by FRET biosensors
  4. 1997 High

    Demonstrated that CDK1 INACTIVATION, not just activation, is mechanistically required—non-degradable cyclin B blocks anaphase spindle dynamics, decondensation, NE reformation, and cytokinesis.

    Evidence Microinjection of non-destructible cyclin BΔ90 with tubulin and F-actin/myosin imaging in live cells

    PMID:9230080

    Open questions at the time
    • Direct substrates whose dephosphorylation drives exit not identified here
    • Phosphatase switches characterized only later
  5. 2013 High

    Identified the substrate-level basis for cohesion release, condensation, and recombination control, linking CDK1 phosphorylation directly to chromosome structural transitions.

    Evidence In vitro kinase assays, phospho-site mutagenesis, and functional cohesion/condensation/recombination readouts (Sororin, Smc4, Mus81-Mms4) across human and yeast systems

    PMID:21878504 PMID:23531881 PMID:23901111 PMID:25691469

    Open questions at the time
    • Quantitative thresholds distinguishing arm versus centromeric cohesion not fully mapped
    • Counteracting phosphatase kinetics partially characterized
  6. 2019 High

    Mapped the kinetochore signaling logic by which CDK1 both primes PLK1 docking and sustains the spindle assembly checkpoint, including a CDK1-MAD1 positive feedback loop and MPS1 S281 phosphorylation.

    Evidence Phospho-site mutagenesis, ectopic localization, reconstitution, Co-IP, and live imaging at kinetochores (BUB1/CENP-U, CLASP2, MPS1, MAD1, Dam1, Nsk1)

    PMID:22065639 PMID:23045552 PMID:30674582 PMID:30674583 PMID:32946748 PMID:33248027

    Open questions at the time
    • Hierarchy among multiple kinetochore substrates during attachment maturation not fully ordered
    • Spatial restriction of CDK1 activity at individual kinetochores not directly measured
  7. 2015 High

    Extended CDK1 function beyond chromosome mechanics to metabolic and translational adaptation during mitosis, showing it sustains cap-dependent translation and boosts mitochondrial output.

    Evidence In vitro kinase assays, mutagenesis, translation reconstitution, and mitochondrial functional assays (4E-BP1, LARP1, SIRT3, complex I subunits)

    PMID:25883264 PMID:26141949 PMID:26670043 PMID:32040547

    Open questions at the time
    • In vivo contribution of mitotic translation maintenance to fitness not quantified
    • Triggers relocalizing CDK1 to mitochondria incompletely defined
  8. 2020 High

    Defined the phosphatase-switch machinery of mitotic exit, showing CDK1 controls PP1/PP2A recruitment and holoenzyme assembly to set the timing of substrate dephosphorylation.

    Evidence In vitro kinase/phosphatase assays, chemical proteomics, mutagenesis, and live-cell mitotic-exit imaging (RepoMan, PP2Ac/B55, NuMA, cGAS via PP1)

    PMID:26674376 PMID:32351706 PMID:32591484 PMID:32900880

    Open questions at the time
    • Integrated kinetic model linking CDK1 inactivation to ordered substrate dephosphorylation incomplete
    • Spatial regulation of phosphatase pools not fully resolved
  9. 2021 High

    Refined the biochemical model of CDK1 specificity, establishing CKS1 as a processivity enhancer that broadens CDK1 toward non-proline-directed multisite phosphorylation and requires CAK-dependent T-loop phosphorylation.

    Evidence Recombinant binary/ternary complex reconstitution with CAKs, quantitative phosphoproteomics, and substrate specificity analysis

    PMID:34791727 PMID:36840943

    Open questions at the time
    • Structural basis of CKS1-directed specificity not resolved here
    • In vivo prevalence of non-canonical sites versus canonical sites not quantified
  10. 2022 Medium

    Revealed spatial compartmentalization of CDK1 activity—an inhibited spindle-bound pool and nuclear-cytoplasmic partitioning that confer robustness to the mitotic clock.

    Evidence FRET biosensors, live imaging, and Wee1/CDC25 perturbations in reconstituted cell systems

    PMID:35081344 PMID:36577372

    Open questions at the time
    • Molecular basis for maintaining a locally inhibited spindle CDK1 pool not fully defined
    • Generality of nuclear-frequency robustness across cell types untested
  11. 2023 High

    Documented CDK1's oncogenic substrate network, in which it directs degradation of tumor-suppressive substrates and activates deubiquitinases stabilizing pro-tumor factors, while being stabilized itself by deubiquitinases.

    Evidence In vitro kinase, ubiquitination/deubiquitination assays, CRISPR screens, and xenograft models (ACSL4, pVHL, USP29/TWIST1, USP9X/WT1, kindlin; YOD1/OTUD4/USP14 stabilizing CDK1)

    PMID:32152317 PMID:35469017 PMID:36782089 PMID:36813923 PMID:37428466 PMID:37667382 PMID:38429268

    Open questions at the time
    • Whether these activities are mitosis-restricted or interphase functions often unclear
    • Several CDK1-stabilizing DUB studies rest on Co-IP without reconstitution

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the hundreds of CDK1 substrates are temporally ordered—via differential site affinity, CKS1 processivity, compartmentalization, and opposing phosphatase kinetics—into a single coherent mitotic program remains unresolved.
  • No unified quantitative model linking CDK1 activity thresholds to substrate ordering
  • Spatial pools of active versus inhibited CDK1 not mapped genome-wide to substrates
  • Relative in vivo importance of non-cell-cycle functions versus mitotic functions unquantified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 8 GO:0140096 catalytic activity, acting on a protein 8 GO:0140657 ATP-dependent activity 2
Localization
GO:0005634 nucleus 3 GO:0005739 mitochondrion 3 GO:0005856 cytoskeleton 3 GO:0005815 microtubule organizing center 1
Pathway
R-HSA-1640170 Cell Cycle 5 R-HSA-5357801 Programmed Cell Death 3 R-HSA-73894 DNA Repair 3 R-HSA-1430728 Metabolism 2 R-HSA-392499 Metabolism of proteins 2 R-HSA-168256 Immune System 1 R-HSA-9612973 Autophagy 1
Partners
CCNB1CDC25CKS1MAD1MPS1PLK1PP2A-B55WEE1
Complex memberships
CDK1-cyclin ACDK1-cyclin B-CKS1MPF (CDK1-cyclin B)

Evidence

Reading pass · 65 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 CDK1 (Cdc2) forms a stoichiometric heterodimeric complex with cyclin B, and the cyclin subunit is necessary for CDK1 to gain protein kinase activity; this complex constitutes MPF (maturation-promoting factor) that controls entry into M phase. Biochemical purification, sequencing of cyclin B from purified starfish MPF, reconstitution in invertebrate eggs and vertebrate cells Journal of cell science High 12045216
2005 CDK1 directly phosphorylates Swe1 (yeast Wee1), first activating Swe1 and promoting formation of a stable Swe1-CDK1 inhibitory complex, and then further phosphorylating Swe1 to release and activate CDK1; thus CDK1 both positively and negatively regulates its own inhibitor. In vitro kinase assay, co-immunoprecipitation, phosphorylation site mutagenesis in budding yeast Cell High 16096060
2004 Drosophila Wee1 (dWee1) phosphorylates CDK1 at tyrosine 15 to inhibit its activity and time mitotic entry; loss of maternal dwee1 causes premature mitosis, spindle defects, and embryonic lethality. Genetic loss-of-function (dwee1 mutant embryos), in vitro kinase assay, Chk2-dependent developmental block as phenotypic readout Current biology High 15589158
2003 Binding of cyclin B1 to CDK1 initiates conformational changes allowing CDK1 to alter its phosphorylation status and become an active kinase; the active cyclin B1-CDK1 complex must translocate to the nucleus (via phosphorylation of cyclin B1 within the CRS region) to phosphorylate nuclear substrates at mitotic onset. Biochemical analysis, phosphorylation studies, nuclear import/export assays Progress in cell cycle research Medium 14593728
1997 CDK1 inactivation (by expression of non-destructible cyclin B) inhibits chromosome decondensation, nuclear envelope formation, and cytokinesis, and prevents formation of the central anaphase spindle and equatorial organization of F-actin/myosin II, demonstrating that CDK1 inactivation is required to drive anaphase spindle dynamics and cytokinesis. mRNA microinjection of non-destructible cyclin BΔ90 into prometaphase cells, rhodamine-tubulin injection, F-actin/myosin II localization by immunofluorescence The Journal of cell biology High 9230080
2013 Aurora B and CDK1 phosphorylate Sororin, which destabilizes the Sororin-Pds5 interaction, releases acetylated cohesin from chromosome arms, and promotes loss of cohesion; centromeric PP2A-Sgo1 opposes this by dephosphorylating Sororin. In vitro kinase assay, co-immunoprecipitation, phospho-site mutagenesis, cell-based chromosome cohesion assays Proceedings of the National Academy of Sciences High 23901111
2011 CDK1 phosphorylates Sororin, causing its release from chromatin and cohesin in mitosis; hypophosphorylated Sororin associates with DNA and cohesin and increases sister chromatid cohesion, while CDK1-phosphorylated Sororin loses chromatin association. CDK1 phosphorylation site mutagenesis, DNA-cellulose pull-down, co-immunoprecipitation, sister chromatid cohesion assays Journal of cell science High 21878504
2007 G1 cyclin-CDK1 complexes in budding yeast specifically phosphorylate multiple proteins associated with Cdc24 (the GEF for Cdc42), and a Cdc24-associated protein with mutated CDK1 phosphorylation sites causes bud growth defects, linking CDK1 directly to control of polarized cell growth. Mass spectrometry identification of CDK1 substrates, phosphorylation site mutagenesis, bud growth phenotype analysis Nature cell biology High 17417630
2013 CDK1 (with Cdc5/PLK1) activates Mus81-Mms4 via phosphorylation in late G2/M to drive resolution of DNA damage-tolerance recombination intermediates; premature activation of this pathway causes crossover-associated chromosomal translocations. Phosphomimetic/phospho-deficient Mms4 variants, genetic epistasis in S. cerevisiae, checkpoint-deficient backgrounds The EMBO journal High 23531881
2015 CDK1-cyclin B1 directly hyperphosphorylates 4E-BP1 at authentic mTOR phosphorylation sites during mitosis, generating a mitosis-specific δ-isoform resistant to mTOR inhibition; this maintains cap-dependent protein translation during mitosis. In vitro kinase assay with recombinant CDK1/CYCB1 and 4E-BP1, mTOR inhibitor resistance assay, reticulocyte translation reconstitution, Click-iT flow cytometry for protein synthesis Proceedings of the National Academy of Sciences High 25883264
2018 CDK1 in complex with cyclin A2 promotes adhesion complex organization and actin cytoskeleton organization during interphase (S phase); elevated cyclin B1 and subsequent inhibitory phosphorylation of CDK1 triggers adhesion complex disassembly in G2 and entry into mitosis. Cell synchronization, CDK1 inhibition, cyclin knockdown/overexpression, high-content imaging of focal adhesion dynamics The Journal of cell biology High 29930204
2020 CDK1-cyclin B phosphorylates human cGAS at S305 (mouse S291), inhibiting its ability to synthesize cGAMP during mitosis and thus preventing innate immune sensing of self-DNA upon nuclear envelope breakdown; PP1 dephosphorylates cGAS upon mitotic exit to restore DNA sensing. In vitro kinase assay, phospho-site mutagenesis, cGAMP synthesis assay, cell-based mitotic entry/exit experiments Cell discovery High 32351706
2018 CDK1 (priming kinase) phosphorylates BUB1 and CENP-U at PLK1-docking sites at kinetochores; this CDK1-primed phosphorylation enables PLK1 recruitment to kinetochores and promotes PLK1 dimerization, constituting the main PLK1 kinetochore-targeting mechanism. Ectopic localization assay, in vitro reconstitution, kinetochore localization studies, phospho-site mutagenesis Molecular cell High 33248027
2019 CDK1-CCNB1 phosphorylates MPS1 at S281, enabling MPS1 recruitment to unattached kinetochores and spindle checkpoint signaling; PP2A-B55, negatively regulated by CDK1-CCNB1, delays MPS1 S281 dephosphorylation to extend the checkpoint-responsive period. Phospho-site mutagenesis, kinetochore tethering bypass experiments, cell cycle synchronization, kinase/phosphatase inhibition The Journal of cell biology High 30674582
2019 CDK1-CCNB1 localizes to unattached kinetochores via interaction with the first 100 amino acids of MAD1 (in an MPS1-dependent manner), creating a positive feedback loop for MPS1 kinetochore recruitment and sustained spindle checkpoint arrest. Proteomic analysis, co-immunoprecipitation, kinetochore localization studies, live cell imaging The Journal of cell biology High 30674583
2020 In embryonic stem cells, CDK1 phosphorylates chromatin-bound proteins including Dot1l (H3K79 methyltransferase), partially inactivating it; decrease in CDK1 activity during differentiation de-represses Dot1l and allows coordinated expression of differentiation genes. Analog-sensitive CDK1 knockin mice, quantitative phosphoproteomics, histone modification profiling, Dot1l activity assays Molecular cell High 32240602
2015 CDK1-cyclin B1 phosphorylates SIRT3 at Thr150/Ser159, enhancing SIRT3 deacetylase activity in mitochondria; cells expressing Thr150Ala/Ser159Ala-mutant SIRT3 show reduced mitochondrial deacetylation, membrane potential, MnSOD activity, and ATP generation, and increased radiation sensitivity. In vitro kinase assay, phospho-site mutagenesis, mitochondrial fractionation, mitochondrial functional assays, clonogenic survival, xenograft Molecular cancer therapeutics High 26141949
2015 CDK1 relocates to mitochondria upon genotoxic stress and phosphorylates complex I subunits, boosting mitochondrial ATP generation to fuel nuclear DNA repair; cells with CDK1 phosphorylation-deficient complex I subunits show compromised mitochondrial ATP and DNA repair. Mitochondrial fractionation, in vitro kinase assay, phospho-site mutagenesis of complex I subunits, oxygen consumption and ATP measurement, DNA repair assay Cell reports High 26670043
2012 During mitosis, CDK1 phosphorylates CLASP2 at S1234, priming CLASP2 for association with PLK1; PLK1 recruitment to kinetochores is enhanced by this phosphorylation and is required to stabilize kinetochore-microtubule attachments for chromosome alignment. In vitro kinase assay, phospho-site mutagenesis, kinetochore localization, chromosome alignment assays The Journal of cell biology High 23045552
2015 CDK1 directly phosphorylates Ajuba at Ser119 and Ser175 during G2/M; mitotic phosphorylation of Ajuba is sufficient to promote cell proliferation and anchorage-independent growth in vitro and tumorigenesis in vivo, independently of Hippo pathway effects. In vitro kinase assay, phospho-site mutagenesis, cell proliferation assays, anchorage-independent growth, in vivo tumorigenesis The Journal of biological chemistry High 27226586
2015 CDK1 phosphorylates TAZ at S90, S105, T326, and T346 during G2/M, inactivating TAZ oncogenic activity; non-phosphorylatable TAZ-5A has higher transcriptional activity and causes greater spindle/centrosome defects than wild-type TAZ. In vitro kinase assay, phospho-site mutagenesis, EMT assay, anchorage-independent growth, mitotic spindle analysis Oncotarget High 26375055
2015 CDK1 phosphorylates the Smc4 subunit of condensin at multiple sites; this multisite phosphorylation acts as a high-sensitivity switch that drives chromosome condensation at mitotic entry, and its abrogation causes chromosome segregation defects and lethality. Phospho-site mutagenesis of Smc4, CDK1 activity titration, chromosome segregation assays in budding yeast Genes & development High 25691469
2016 CDK1-CyclinB inhibits PLK4-STIL complex formation in mitosis by binding STIL, thereby preventing untimely centriole biogenesis; after CDK1-CyclinB inactivation at mitotic exit, PLK4 can bind and phosphorylate STIL in G1 to initiate pro-centriole assembly. Co-immunoprecipitation, phosphorylation assays, in vitro kinase assay, cell cycle synchronization experiments Current biology High 27112295
2016 CDK1 phosphorylates WRN at S1133, promoting WRN interaction with the MRE11 complex and enabling DNA2-dependent long-range resection at replication-associated DSBs, thereby promoting homologous recombination and suppressing NHEJ. In vitro kinase assay, phospho-site mutagenesis, co-immunoprecipitation (WRN-MRE11), DNA resection assays, HR/NHEJ pathway choice assay Nature communications High 27634057
2020 CDK1 phosphorylates hTERT at threonine 249 during mitosis; this phosphorylation is required for hTERT RNA-dependent RNA polymerase (RdRP) activity but dispensable for reverse transcriptase activity, and regulates expression of genes necessary for cancer cell proliferation. CRISPR/Cas9 endogenous mutagenesis, in vitro kinase assay, CAGE transcriptomics, RdRP activity assay Nature communications High 32214089
2020 CDK1 phosphorylates ULK1 and ATG13 (of the ULK1-ATG13 autophagy initiation complex) during mitosis, promoting mitotic autophagy and cell cycle progression; double knockout of ULK1 and ATG13 blocks cell cycle progression. Mass spectrometry, site-directed mutagenesis, in vitro kinase assay, ULK1/ATG13 double KO mouse/cell models PLoS biology High 32516310
2020 CDK1 phosphorylates LARP1, and this phosphorylation is required for CDK1's strong positive effect on translation of 5'TOP mRNAs (encoding ribosomal proteins and translation factors), coupling cell proliferation with biosynthesis of the translation machinery. Kinase/phosphatase screen, cell synchronization, Ribo-Seq, LARP1 phosphorylation analysis, stress granule formation assay The Journal of cell biology High 32040547
2014 CDK1/cyclin B1 phosphorylates procaspase-8 at S387, creating a phospho-epitope for PLK1's polo-box domain binding; subsequent PLK1 phosphorylation of S305 inhibits caspase-8 activation, reducing sensitivity to extrinsic apoptosis during mitosis. In vitro kinase assay, RNAi replacement with phospho-mutants, Fas-stimulated death receptor assay Molecular oncology High 24484936
2015 CDK1 phosphorylation of RepoMan reduces PP1 binding to RepoMan but facilitates PP2A-B56 recruitment; upon CDK1 inactivation in early anaphase, this phosphatase switch is reversed, accumulating PP1-RepoMan to inactivate Aurora B and initiate nuclear envelope reassembly. In vitro kinase assay, phospho-site mutagenesis, phosphatase binding assays, live-cell imaging of mitotic exit events Nature communications High 26674376
2020 CDK1 phosphorylates the PP2A catalytic subunit (PP2Ac) at a threonine residue, disrupting its holoenzyme formation with regulatory subunit B55, thereby decreasing dephosphorylation of PP2A-B55 substrates and promoting mitotic entry. Chemical proteomics enrichment of PPP holoenzymes, mass spectrometry, kinase profiling, in vitro phosphorylation Science signaling High 32900880
2008 CDK1 directly phosphorylates the vacuole inheritance adaptor Vac17 in budding yeast, promoting Vac17 interaction with the myosin V motor Myo2 and coordinating vacuole movement with cell cycle progression. In vitro kinase assay, phospho-site mutagenesis, co-immunoprecipitation, vacuole inheritance assay Developmental cell High 18804442
2017 CDK1-cyclin B activity generates a spatiotemporal gradient from the nucleus in starfish oocytes that drives surface contraction waves by activating RhoA/RhoA kinase/myosin II at the wave front (via removal of CDK1 inhibition of RhoA module) and negative feedback at the rear. Quantitative live imaging, biochemical perturbations, mathematical modeling, CDK1 activity manipulation Nature communications High 29021609
2012 CDK1 phosphorylates KIBRA at Ser542 and Ser931 in response to spindle damage; CDC14A/B phosphatases associate with KIBRA and dephosphorylate these CDK1 sites; this CDK1/CDC14-dependent phosphoregulation of KIBRA is involved in mitotic exit under spindle stress. In vitro kinase assay, phospho-site mutagenesis, co-immunoprecipitation, in vitro phosphatase assay, inducible expression cell lines The Biochemical journal High 22784093
2019 CDK1 phosphorylates TFCP2L1 (a pluripotency transcription factor) at Thr177, affecting ESC cell cycle progression, pluripotency, and differentiation; CDK1-mediated TFCP2L1 phosphorylation also drives bladder cancer cell proliferation, self-renewal, and invasion. In vitro kinase assay, phospho-site mutagenesis (Thr177), ESC differentiation assays, xenograft tumor model EMBO molecular medicine High 31709755
2014 CDK1 (and PKCδ) bind Drp1 and phosphorylate it at Ser616, activating Drp1-mediated mitochondrial fission; inhibition of CDK1 attenuates pSer616 Drp1, mitochondrial fission, and cardiomyocyte death during anoxia-reoxygenation injury. Co-immunoprecipitation, phospho-specific antibody detection, CDK1 inhibitor treatment, mitochondrial morphology assay, cell death assay Biochemical and biophysical research communications Medium 25445585
2016 CDK1 phosphorylates Bora (SPAT-1 in C. elegans) at conserved Sp/Tp residues, which is essential for Aurora A-dependent PLK1 activation and mitotic entry; this mechanism also operates during DNA damage checkpoint recovery in mammalian cells. Phospho-site mutagenesis of Bora, FRET-based PLK1 activity biosensor, C. elegans genetics, mammalian checkpoint recovery assays Cell cycle High 27831827
2019 CDK1 and CDK2 phosphorylate NICD1 (Notch intracellular domain), targeting it for SCF E3 ligase-dependent degradation in a cell cycle-dependent manner; inhibiting CDK1 or CDK2 increases NICD levels, delays segmentation clock gene oscillations, and increases somite size. In vitro kinase assay, phospho-site mutagenesis, CDK inhibitor treatment, in vivo somitogenesis assay EMBO reports High 31267714
2021 CDK1 phosphorylates CHMP7 at Ser3 and Ser441 upon mitotic entry, reducing CHMP7 interaction with LEM2 and suppressing ESCRT-III assembly during M phase; spatiotemporal dephosphorylation of CHMP7 by CDK1 inactivation licenses ESCRT-III-dependent nuclear envelope reformation during telophase. Live cell imaging, protein biochemistry, phospho-site mutagenesis, CDK1 inhibition eLife High 34286694
2019 CDK1-cyclin B1 phosphorylates importin-α1 at Thr9 and Ser62 during mitosis, inhibiting its binding to importin-β and promoting release of spindle assembly factors TPX2 and KIFC1 to the spindle; non-phosphorylatable importin-α1 causes shortened spindles and prolonged metaphase. Phospho-site mutagenesis, importin-β binding assay, spindle assembly phenotype analysis, live cell imaging Journal of cell science High 31434716
2020 CDK1 phosphorylates NuMA at Thr2055, negatively regulating its cortical localization; PP2A-B55γ (PPP2CA-B55γ-PPP2R1B complex) dephosphorylates NuMA T2055 to restore cortical dynein and ensure proper mitotic spindle orientation. In vitro reconstitution of phosphatase activity, phospho-site mutagenesis, cortical NuMA localization assay, spindle orientation phenotype Journal of cell science High 32591484
2022 CDK1-cyclin B1 phosphorylates kindlin during mitotic entry, leading to recruitment of the cullin 9-FBXL10 ubiquitin ligase complex that ubiquitinates and degrades kindlin, driving focal adhesion disassembly and cell rounding at mitotic entry. Phospho-site mutagenesis, co-immunoprecipitation of ubiquitin ligase, ubiquitination assay, FA disassembly phenotype, live cell imaging Nature cell biology High 35469017
2019 CDK1 phosphorylates Sox2, increasing its phosphorylation, nuclear translocation, and transcriptional activity; blockade of CDK1 reduces Sox2 nuclear localization and activity, and knockout of Sox2 abolishes CDK1-driven tumor-initiating capacity in melanoma. Proteomic analysis (Co-IP-MS), CDK1 overexpression/inhibition, Sox2 knockdown/knockout, nuclear localization assay, sphere-forming and xenograft assays Cancer research Medium 30297536
2017 CDK1-cyclin B1 phosphorylates XIAP at S40, inhibiting XIAP's binding to activated effector caspases and abolishing its anti-apoptotic function; phosphomimetic S40D mutation sensitizes cells to apoptosis during mitotic arrest, while non-phosphorylatable S40A blocks apoptosis. In vitro kinase assay, phospho-site mutagenesis, caspase binding assay, live-cell imaging of apoptosis during mitotic arrest Journal of cell science High 27927753
2017 CDK1 phosphorylates PBK at Thr9, Thr24, Ser32, and Ser59 during mitosis; phosphorylation of Thr9 is required for cytokinesis, and non-phosphorylatable PBK-T9A augments tumorigenesis, indicating CDK1-mediated phosphorylation inhibits PBK's oncogenic activity. In vitro kinase assay, phospho-site mutagenesis, cytokinesis phenotype assay, tumorigenesis assay Cellular signalling High 28780319
2000 CDK1 negatively regulates APC/C activation by the Cdh1/Fzr co-activator, preventing premature cyclin degradation; Fzr overexpression or CDK1 inhibition can override the prometaphase checkpoint, and mammalian Cdc14 phosphatase contributes to this pathway. CDK1 inhibitor treatment, Fzr overexpression, spindle checkpoint assay, mammalian cell-based cyclosome activity assay Experimental cell research High 10694434
2012 CDK1 phosphorylates MYT1 inhibitory kinase contributes context: MYT1 (not WEE1) has a rate-determining role in checkpoint recovery — depletion of MYT1 causes precocious mitotic entry when checkpoint is abrogated by CHK1 or WEE1 inhibitors, by lowering the threshold for CDK1 activation. MYT1 siRNA depletion, CHK1/WEE1 inhibitor treatment, time-lapse microscopy, clonogenic survival, xenograft Oncogene Medium 23146904
2020 CDK1 directly phosphorylates USP9X at serine 2563, activating its deubiquitinase activity; activated USP9X stabilizes WT1 (Wilms' tumor protein 1), which functions as a mitotic transcription factor driving CXCL8/IL-8 expression for mitotic survival; CDC14B phosphatase opposes this by dephosphorylating USP9X. Proteome-wide phosphorylation screen, in vitro kinase assay, ubiquitination/deubiquitination assays, WT1 transcription assays Nature communications High 32152317
2021 USP7 interacts with PP2A and supports active PP2A localization in the cytoplasm; inhibition of USP7 leads to widespread CDK1 activation throughout the cell cycle, producing CDK1 target phosphorylation and DNA damage; toxicity is alleviated by lowering CDK1 activity or activating PP2A. USP7 inhibitor treatment, CDK1 inhibitor rescue, phosphoproteomics, co-immunoprecipitation (USP7-PP2A) The EMBO journal Medium 33856059
2020 CDK1 phosphorylates the Dam1 complex subunit Ask1 in budding yeast, increasing Dam1 complex residence time on microtubules and enhancing kinetochore-microtubule attachment strength via promotion of Dam1 oligomerization. In vitro reconstitution with purified components, single-molecule microtubule attachment assay, phospho-site mutagenesis of Ask1 Current biology High 32946748
2021 CKS1 has a proteome-wide role as an enhancer of multisite CDK1 phosphorylation; Cyclin A and CKS1 together promote CDK1 phosphorylation at non-proline-directed sites (preferably with +3 lysine), contrary to the classical model of CDK1 as exclusively proline-directed. Fixed-cell kinase assay, quantitative phosphoproteomics, recombinant CDK1 complex reconstitution with Cyclin A/B and CKS1 Cell reports High 36840943
2022 A fraction of CDK1 bound to spindle structures is maintained in an inhibited (phosphorylated) state by Wee1 even in mitosis; this 'compartmentalized' inactive CDK1 (i-Cdk1) is required for spindle assembly, as loss of i-Cdk1 inhibits spindle assembly and its restoration promotes it. Live cell imaging, immunofluorescence, CDK1 inhibitor and Wee1 inhibitor treatment, Cdc25 exclusion from spindle-bound CDK1 Cell reports Medium 35081344
2022 Nuclear-cytoplasmic compartmentalization of cyclin B1-CDK1 modulates cell cycle clock properties: in nucleus-present cells, CDK1 frequency remains constant against cyclin B1 variations (robust mitotic phase duration), and active cyclin B1-CDK1 accumulates in nuclei without delay until nuclear envelope breakdown triggers anaphase. FRET biosensor for CDK1 activity in reconstituted cells with or without nucleus, quantitative live-cell imaging Cell reports High 36577372
2021 Reconstituted ternary CDK1:Cyclin-B:CKS1 complex is more active than binary CDK1:Cyclin-B; CDK1 kinase activity requires T-loop phosphorylation by CDK-activating kinase (CAK); CKS1 boosts CDK1 processivity for multisite substrate phosphorylation. Recombinant protein reconstitution in insect cells and in vitro with purified CAKs, in vitro kinase assay comparing binary/ternary complexes Protein science High 34791727
2022 S6K1 phosphorylates CDK1 at serine 39, causing G2/M cell cycle arrest and enabling homologous recombination DNA repair; this places CDK1 downstream of the mTORC1-S6K1 pathway as a regulator of DNA repair pathway choice. In vitro kinase assay, phospho-site mutagenesis, cell cycle analysis, HR repair assay eLife Medium 36189922
2023 CDK1 directly binds and phosphorylates ACSL4 at S447, recruiting E3 ubiquitin ligase UBR5 and causing polyubiquitination and degradation of ACSL4, thereby reducing polyunsaturated fatty acid lipid biosynthesis, inhibiting ferroptosis, and conferring oxaliplatin resistance in colorectal cancer. Co-immunoprecipitation, in vitro kinase assay, ubiquitination assay, CRISPR/Cas9 screen, cell/patient-derived xenograft models Advanced science High 37428466
2023 CDK1 directly phosphorylates USP29, activating its deubiquitinase activity toward TWIST1, stabilizing TWIST1 and driving EMT, cancer stem cell features, and chemotherapy resistance in triple-negative breast cancer. In vitro kinase assay, deubiquitinase activity assay, co-immunoprecipitation, TWIST1 stability analysis, in vitro/in vivo tumor models Advanced science High 36782089
2023 CDK1 directly phosphorylates pVHL at Ser80, priming recognition by PIN1 isomerase, which then facilitates recruitment of E3 ligase WSB1, leading to pVHL ubiquitination and degradation, thereby promoting tumor progression. In vitro kinase assay, co-immunoprecipitation, ubiquitination assay, CDK1 inhibitor (RO-3306) treatment, in vivo xenograft Cell death and differentiation High 36813923
2023 YOD1 (OTUD2) deubiquitinase binds CDK1, removes its ubiquitin chains, and stabilizes CDK1 protein by preventing its degradation; this stabilization promotes TNBC proliferation and tumor progression. Co-immunoprecipitation, ubiquitination/deubiquitination assay, YOD1 knockdown, in vitro and in vivo tumor models Journal of experimental & clinical cancer research Medium 37667382
2024 OTUD4 deubiquitinase directly interacts with CDK1 and stabilizes it by removing K11-, K29-, and K33-linked polyubiquitin chains; OTUD4 also indirectly stabilizes CDK1 via FGFR1 stabilization, resulting in MAPK pathway activation and GBM progression. Co-immunoprecipitation, ubiquitination assay (linkage-specific), in vitro DUB assay, in vitro/in vivo GBM models Cell death & disease Medium 38429268
2022 USP14 deubiquitinase interacts with CDK1 and stabilizes it by removing K48-linked ubiquitin chains, preventing CDK1 degradation; USP14 inhibition causes G2/M arrest and reduces CDK1 protein levels in breast cancer cells. Co-immunoprecipitation, K48-linked ubiquitination assay, flow cytometry cell cycle analysis, CDK1 protein stability assay Acta biochimica et biophysica Sinica Medium 36604147
2018 DNA replication provides an inhibitory signal that restricts CDK1 and PLK1 activation; prevention of DNA replication licensing/firing leads to premature CDK1 and PLK1 activation in S phase, and CHK1/p38 inhibition in the presence of ongoing replication induces severe replication stress via premature CDK1/PLK1 activation. Double-degron degradation system, kinase inhibitors, DNA replication inhibition, CDK1/PLK1 activity monitoring Molecular cell High 30008317
2011 CDK1 phosphorylation of the kinetochore protein Nsk1 in fission yeast antagonizes its kinetochore and spindle localization during early mitosis; non-phosphorylatable Nsk1 binds prematurely, cementing improper kinetochore-microtubule attachments and causing chromosome mis-segregation. Phospho-site mutagenesis of Nsk1, kinetochore/spindle localization assay, chromosome segregation phenotype analysis in S. pombe The Journal of cell biology High 22065639
2019 CDK1 phosphorylates the transcription factor Hcm1 at distinct site clusters in budding yeast: one cluster activates Hcm1 (promoting S-phase gene expression) and another targets it for degradation; calcineurin phosphatase specifically removes activating phosphates to slow proliferation under stress. Phospho-site mutagenesis, Hcm1 transcriptional activity assay, calcineurin inhibition, stress response assays Molecular biology of the cell / Current genetics Medium 26269584 26590602
2021 CDK1-Clb2-Cks1 multisite phosphorylation of the transcriptional co-activator Ndd1 first promotes CLB2 gene transcription (positive feedback at low CDK1 activity) and then, at high CDK1 activity, triggers Ndd1 degradation (delayed negative feedback), producing a pulse of mitotic gene expression. Phospho-site mutagenesis of Ndd1, CLB2 reporter assay, CDK1 activity titration, Cks1/Clb2 phosphate-binding pocket mutations Current biology High 34818519
2017 CDK1 and PLK1 coordinate disassembly and reassembly of the nuclear envelope during vertebrate mitosis via independent regulatory pathways: Lamin A/C targeting to chromatin is controlled by CDK1 activity (clock-based model), while NPC loading is spatially monitored by PLK1. CDK1 and PLK1 inhibitor treatment, live cell imaging of NE components, micronuclei analysis Oncotarget Medium 29487689

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2010 An overview of Cdk1-controlled targets and processes. Cell division 329 20465793
2002 From Cdc2 to Cdk1: when did the cell cycle kinase join its cyclin partner? Journal of cell science 217 12045216
2005 Cdk1-dependent regulation of the mitotic inhibitor Wee1. Cell 176 16096060
2003 Cyclin B1 and CDK1: nuclear localization and upstream regulators. Progress in cell cycle research 164 14593728
2023 Inhibition of CDK1 Overcomes Oxaliplatin Resistance by Regulating ACSL4-mediated Ferroptosis in Colorectal Cancer. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 145 37428466
2023 Targeting CDK1 in cancer: mechanisms and implications. NPJ precision oncology 144 37311884
1997 CDK1 inactivation regulates anaphase spindle dynamics and cytokinesis in vivo. The Journal of cell biology 143 9230080
2013 Aurora B and Cdk1 mediate Wapl activation and release of acetylated cohesin from chromosomes by phosphorylating Sororin. Proceedings of the National Academy of Sciences of the United States of America 130 23901111
2014 Cdk1, PKCδ and calcineurin-mediated Drp1 pathway contributes to mitochondrial fission-induced cardiomyocyte death. Biochemical and biophysical research communications 127 25445585
2007 Cdk1 coordinates cell-surface growth with the cell cycle. Nature cell biology 119 17417630
2020 Phosphorylation of cGAS by CDK1 impairs self-DNA sensing in mitosis. Cell discovery 117 32351706
2018 Cell adhesion is regulated by CDK1 during the cell cycle. The Journal of cell biology 117 29930204
2013 Premature Cdk1/Cdc5/Mus81 pathway activation induces aberrant replication and deleterious crossover. The EMBO journal 115 23531881
2015 CDK1 substitutes for mTOR kinase to activate mitotic cap-dependent protein translation. Proceedings of the National Academy of Sciences of the United States of America 111 25883264
2018 CDK1 Interacts with Sox2 and Promotes Tumor Initiation in Human Melanoma. Cancer research 105 30297536
2018 DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation. Molecular cell 100 30008317
2020 CDK1/2/5 inhibition overcomes IFNG-mediated adaptive immune resistance in pancreatic cancer. Gut 96 32816920
2020 BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis. Molecular cell 91 33248027
2020 Cdk1 Controls Global Epigenetic Landscape in Embryonic Stem Cells. Molecular cell 89 32240602
2015 MicroRNA-490-3P targets CDK1 and inhibits ovarian epithelial carcinoma tumorigenesis and progression. Cancer letters 88 25819031
2015 CDK1-Mediated SIRT3 Activation Enhances Mitochondrial Function and Tumor Radioresistance. Molecular cancer therapeutics 85 26141949
2020 CDK1 couples proliferation with protein synthesis. The Journal of cell biology 82 32040547
2015 CDK1 Enhances Mitochondrial Bioenergetics for Radiation-Induced DNA Repair. Cell reports 81 26670043
2012 Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore-microtubule attachments. The Journal of cell biology 79 23045552
2020 CDK1 in Breast Cancer: Implications for Theranostic Potential. Anti-cancer agents in medicinal chemistry 72 32013835
2019 MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling. The Journal of cell biology 72 30674583
2006 Discovery and evaluation of dual CDK1 and CDK2 inhibitors. Cancer research 72 16618755
2012 The CDK1 inhibitory kinase MYT1 in DNA damage checkpoint recovery. Oncogene 71 23146904
2019 Orchestration of the spindle assembly checkpoint by CDK1-cyclin B1. FEBS letters 67 31469407
2019 Myc stimulates cell cycle progression through the activation of Cdk1 and phosphorylation of p27. Scientific reports 67 31822694
2004 Drosophila Wee1 kinase regulates Cdk1 and mitotic entry during embryogenesis. Current biology : CB 67 15589158
2015 Cdk1 orders mitotic events through coordination of a chromosome-associated phosphatase switch. Nature communications 65 26674376
2014 Sequential Cdk1 and Plk1 phosphorylation of caspase-8 triggers apoptotic cell death during mitosis. Molecular oncology 65 24484936
2016 CDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesis. Current biology : CB 62 27112295
2022 Targeting cyclin-dependent kinase 1 (CDK1) in cancer: molecular docking and dynamic simulations of potential CDK1 inhibitors. Medical oncology (Northwood, London, England) 61 35723742
2011 Regulation of sororin by Cdk1-mediated phosphorylation. Journal of cell science 61 21878504
2019 Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis. EMBO molecular medicine 59 31709755
2021 CDK1 promotes the stemness of lung cancer cells through interacting with Sox2. Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 56 33721187
2017 A cdk1 gradient guides surface contraction waves in oocytes. Nature communications 55 29021609
2016 CDK1 phosphorylates WRN at collapsed replication forks. Nature communications 55 27634057
2020 CDK1 dependent phosphorylation of hTERT contributes to cancer progression. Nature communications 54 32214089
2019 CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization. The Journal of cell biology 51 30674582
2023 Deubiquitylase YOD1 regulates CDK1 stability and drives triple-negative breast cancer tumorigenesis. Journal of experimental & clinical cancer research : CR 48 37667382
2020 ULK1-ATG13 and their mitotic phospho-regulation by CDK1 connect autophagy to cell cycle. PLoS biology 48 32516310
2015 A high-sensitivity phospho-switch triggered by Cdk1 governs chromosome morphogenesis during cell division. Genes & development 46 25691469
2022 CDK1-cyclin-B1-induced kindlin degradation drives focal adhesion disassembly at mitotic entry. Nature cell biology 45 35469017
2025 PSMD12 promotes hepatocellular carcinoma progression by stabilizing CDK1. Frontiers in immunology 43 40534847
2023 Phosphorylation of USP29 by CDK1 Governs TWIST1 Stability and Oncogenic Functions. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 43 36782089
2021 ESRRA promotes gastric cancer development by regulating the CDC25C/CDK1/CyclinB1 pathway via DSN1. International journal of biological sciences 43 34131395
2016 Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis. The Journal of biological chemistry 43 27226586
2017 Residual Cdk1/2 activity after DNA damage promotes senescence. Aging cell 41 28345297
2020 CDK1, the Other 'Master Regulator' of Autophagy. Trends in cell biology 40 33272830
2000 Cdk1 is essential for mammalian cyclosome/APC regulation. Experimental cell research 40 10694434
2008 The cyclin-dependent kinase Cdk1 directly regulates vacuole inheritance. Developmental cell 39 18804442
2021 CDK1 controls CHMP7-dependent nuclear envelope reformation. eLife 37 34286694
2015 The clinical significance of CDK1 expression in oral squamous cell carcinoma. Medicina oral, patologia oral y cirugia bucal 36 25129248
2002 Multiple Cdk1 inhibitory kinases regulate the cell cycle during development. Developmental biology 35 12217326
2017 CDK1 and PLK1 coordinate the disassembly and reassembly of the nuclear envelope in vertebrate mitosis. Oncotarget 34 29487689
2009 Cdc28/Cdk1 positively and negatively affects genome stability in S. cerevisiae. The Journal of cell biology 34 19398760
2016 Mitotic entry: The interplay between Cdk1, Plk1 and Bora. Cell cycle (Georgetown, Tex.) 33 27831827
2024 OTUD4 promotes the progression of glioblastoma by deubiquitinating CDK1 and activating MAPK signaling pathway. Cell death & disease 31 38429268
2021 USP7 limits CDK1 activity throughout the cell cycle. The EMBO journal 31 33856059
2017 CDK1 interacts with iASPP to regulate colorectal cancer cell proliferation through p53 pathway. Oncotarget 31 29069733
2012 Phospho-regulation of KIBRA by CDK1 and CDC14 phosphatase controls cell-cycle progression. The Biochemical journal 31 22784093
2019 CDK1 and CDK2 regulate NICD1 turnover and the periodicity of the segmentation clock. EMBO reports 30 31267714
2002 Differential effects of ergosterol and cholesterol on Cdk1 activation and SRE-driven transcription. European journal of biochemistry 30 11895447
2016 Recurrent CDK1 overexpression in laryngeal squamous cell carcinoma. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 29 26912061
2020 Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival. Nature communications 28 32152317
2019 An oncogenic gene, SNRPA1, regulates PIK3R1, VEGFC, MKI67, CDK1 and other genes in colorectal cancer. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 28 31203132
2017 CDK1-mediated mitotic phosphorylation of PBK is involved in cytokinesis and inhibits its oncogenic activity. Cellular signalling 28 28780319
2009 Cdk1 and cell morphology: connections and directions. Current opinion in cell biology 28 19196503
2016 LAR protein tyrosine phosphatase regulates focal adhesions through CDK1. Journal of cell science 27 27352860
2015 CDK1 phosphorylation of TAZ in mitosis inhibits its oncogenic activity. Oncotarget 27 26375055
2003 Chemical and biological profile of dual Cdk1 and Cdk2 inhibitors. Current medicinal chemistry. Anti-cancer agents 27 12678910
2022 Nuclear-cytoplasmic compartmentalization of cyclin B1-Cdk1 promotes robust timing of mitotic events. Cell reports 25 36577372
2019 Phosphorylation of importin-α1 by CDK1-cyclin B1 controls mitotic spindle assembly. Journal of cell science 24 31434716
2021 Reconstitution and use of highly active human CDK1:Cyclin-B:CKS1 complexes. Protein science : a publication of the Protein Society 23 34791727
2020 Recent advances in understanding the role of Cdk1 in the Spindle Assembly Checkpoint. F1000Research 23 32047615
2015 Create, activate, destroy, repeat: Cdk1 controls proliferation by limiting transcription factor activity. Current genetics 22 26590602
2022 Compartmentalized control of Cdk1 drives mitotic spindle assembly. Cell reports 21 35081344
2022 S6K1 phosphorylates Cdk1 and MSH6 to regulate DNA repair. eLife 21 36189922
2022 USP14 regulates cell cycle progression through deubiquitinating CDK1 in breast cancer. Acta biochimica et biophysica Sinica 21 36604147
2021 CDK1 is up-regulated by temozolomide in an NF-κB dependent manner in glioblastoma. Scientific reports 20 33707466
2016 Critical reanalysis of the methods that discriminate the activity of CDK2 from CDK1. Cell cycle (Georgetown, Tex.) 20 26986210
2023 Cyclin A and Cks1 promote kinase consensus switching to non-proline-directed CDK1 phosphorylation. Cell reports 19 36840943
2020 Quantitative kinase and phosphatase profiling reveal that CDK1 phosphorylates PP2Ac to promote mitotic entry. Science signaling 19 32900880
2020 Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments. Current biology : CB 19 32946748
2023 Computational identification of potential inhibitors targeting cdk1 in colorectal cancer. Frontiers in chemistry 18 38099190
2021 DNMT3A Mutation-Induced CDK1 Overexpression Promotes Leukemogenesis by Modulating the Interaction between EZH2 and DNMT3A. Biomolecules 18 34067359
2021 Multisite phosphorylation by Cdk1 initiates delayed negative feedback to control mitotic transcription. Current biology : CB 17 34818519
2019 Integrated assessment of CDK1 upregulation in thyroid cancer. American journal of translational research 17 31934275
2018 Nucleolar integrity during interphase supports faithful Cdk1 activation and mitotic entry. Science advances 17 29881774
2022 DDX21 Interacts with WDR5 to Promote Colorectal Cancer Cell Proliferation by Activating CDK1 Expression. Journal of Cancer 16 35371306
2022 The CDK1-Related lncRNA and CXCL8 Mediated Immune Resistance in Lung Adenocarcinoma. Cells 16 36078096
2018 TIAR marks nuclear G2/M transition granules and restricts CDK1 activity under replication stress. EMBO reports 16 30538118
2015 Hcm1 integrates signals from Cdk1 and calcineurin to control cell proliferation. Molecular biology of the cell 16 26269584
2011 Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. The Journal of cell biology 16 22065639
2023 PIN1 and CDK1 cooperatively govern pVHL stability and suppressive functions. Cell death and differentiation 14 36813923
2016 Phosphorylation of XIAP by CDK1-cyclin-B1 controls mitotic cell death. Journal of cell science 14 27927753
2020 PP2A--B55γ counteracts Cdk1 and regulates proper spindle orientation through the cortical dynein adaptor NuMA. Journal of cell science 13 32591484

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