Affinage

CDK1

Cyclin-dependent kinase 1 · UniProt P06493

Round 2 corrected
Length
297 aa
Mass
34.1 kDa
Annotated
2026-04-28
130 papers in source corpus 62 papers cited in narrative 62 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CDK1 is the master cyclin-dependent serine/threonine kinase that drives mitotic entry and is the only essential cell cycle CDK in mammals, capable of binding all cyclins and substituting for all interphase CDKs through mid-gestation development (PMID:17700700). In complex with cyclin B, CDK1 activity peaks at G2/M and is activated by Cdc25-mediated dephosphorylation of inhibitory T14/Y15 residues placed by Wee1 and Myt1, while S6K1 phosphorylation at S39 and USP7-supported PP2A activity provide additional negative regulation (PMID:7626804, PMID:9552418, PMID:36189922, PMID:33856059). CDK1 phosphorylates a broad substrate network (~200 substrates) to coordinate chromosome condensation, sister chromatid cohesion dissolution via Sororin, nuclear envelope breakdown via lamin A/C, spindle assembly via Eg5, cytokinesis, organelle inheritance, cGAS inactivation during mitosis, SUMOylation through Ubc9, and global translation via LARP1 (PMID:14574415, PMID:8548803, PMID:21878504, PMID:32351706, PMID:22509284, PMID:32040547). Beyond catalytic roles, CDK1 with Cks1 promotes proteasome-mediated transcription in a kinase-independent scaffolding capacity, and compartmentalized pools of inactive CDK1 on the mitotic spindle are required for proper microtubule dynamics (PMID:15629725, PMID:35081344).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1987 High

    Cloning the human cdc2 homologue by complementation of the fission yeast cdc2 mutant established that a conserved cell cycle kinase exists in human cells, opening the gene to molecular study.

    Evidence Functional complementation of S. pombe cdc2 mutant with human cDNA library

    PMID:3553962

    Open questions at the time
    • Biochemical activity and cyclin partners of the human protein were unknown
    • No mammalian loss-of-function data
  2. 1989 High

    Identification of cyclin A and cyclin B as obligate activating partners of p34cdc2, with cell cycle-dependent kinase activity peaking at mitosis, defined CDK1 as the catalytic engine of M-phase promoting factor (MPF).

    Evidence Immunoprecipitation with anti-cyclin antibodies, histone H1 kinase assay, antibody microinjection blocking mitosis in mammalian cells

    PMID:2538242 PMID:2541912 PMID:2570636

    Open questions at the time
    • Regulatory phosphorylation sites on CDK1 itself were not yet mapped
    • Substrate repertoire was unknown beyond histone H1
  3. 1995 High

    Mapping inhibitory phosphorylation at T14 (Wee1/Myt1) and Y15 and showing that Cdc25-mediated dephosphorylation activates CDK1 resolved how the G2/M switch is controlled at the post-translational level.

    Evidence Chromosomal replacement with cdc2 T14A/T14S mutants in fission yeast; in vitro Myt1 kinase assay with mutagenesis

    PMID:7626804 PMID:9552418

    Open questions at the time
    • Spatial regulation of CDK1 activation within the cell was not addressed
    • Additional inhibitory inputs (e.g. from signaling pathways) were unknown
  4. 1997 High

    Demonstrating that sustained CDK1 activity (via non-destructible cyclin B) blocks chromosome decondensation, nuclear envelope reformation, and cytokinesis established that CDK1 inactivation is as critical as its activation for completing cell division.

    Evidence Microinjection of non-destructible cyclin B mRNA into prometaphase NRK cells, live imaging

    PMID:9230080

    Open questions at the time
    • Which CDK1 substrates must be dephosphorylated for mitotic exit was not defined
    • Relative contributions of APC/C-mediated cyclin destruction versus phosphatase reactivation were unclear
  5. 2003 High

    Proteomic identification of ~200 CDK1 substrates in yeast revealed the scale of the mitotic phosphorylation program and shifted the field from single-substrate studies to a systems-level view of CDK1 function.

    Evidence Analog-sensitive CDK1 allele with thiophosphate-ATP labeling in whole-cell extracts, in vivo validation

    PMID:14574415

    Open questions at the time
    • Temporal ordering of substrate phosphorylation was not established
    • Human substrate repertoire was not yet systematically defined
  6. 2005 Medium

    Discovery that CDK1/Cks1 promotes transcription via proteasome recruitment in a kinase-independent manner revealed an unexpected scaffolding function, expanding CDK1 biology beyond catalysis.

    Evidence ChIP, transcriptomics, and kinase-dead allele analysis in budding yeast

    PMID:15629725

    Open questions at the time
    • Whether kinase-independent transcriptional roles are conserved in mammals is untested
    • Mechanism of proteasome-mediated transcription activation is not fully defined
  7. 2007 High

    Compound knockout of all interphase CDKs in mice showed CDK1 alone can drive the entire cell cycle — binding all cyclins and phosphorylating pRb — establishing CDK1 as the only essential mammalian CDK.

    Evidence Cdk2/3/4/6 compound knockout mice; MEF proliferation and pRb phosphorylation analysis

    PMID:17700700

    Open questions at the time
    • Why CDK1 alone is insufficient for normal development despite driving proliferation was unclear
    • Cyclin-specific substrate selectivity of CDK1 was not resolved
  8. 2011 High

    CDK1 phosphorylation of Sororin was shown to dissolve sister chromatid cohesion by disrupting Sororin–cohesin interactions, while Sgo1-PP2A at centromeres opposes this to protect centromeric cohesion until anaphase — resolving how cohesion is differentially regulated on arms versus centromeres.

    Evidence In vitro kinase assay, Sororin phospho-mutants, co-IP with Pds5/cohesin, PP2A dephosphorylation assay

    PMID:21878504 PMID:23901111

    Open questions at the time
    • Quantitative thresholds of CDK1 activity needed for arm versus centromere cohesion resolution were not measured
    • Interplay with Wapl pathway was not fully dissected
  9. 2015 High

    CDK1 was found to relocalize to mitochondria upon stress, phosphorylating complex I subunits and SIRT3 to boost ATP generation — extending CDK1 function beyond canonical nuclear/cytoplasmic cell cycle roles into organellar metabolism.

    Evidence Mitochondrial fractionation, kinase-dead mitochondrially-targeted CDK1, phosphorylation-deficient complex I subunit mutants, oxygen consumption and ATP assays

    PMID:26141949 PMID:26670043

    Open questions at the time
    • How CDK1 is imported into mitochondria is unknown
    • Whether mitochondrial CDK1 activity is cell cycle-regulated or a distinct stress response is unresolved
  10. 2020 High

    Phosphorylation of cGAS at S305 by CDK1 upon mitotic entry was shown to suppress cGAMP synthesis, explaining how cells avoid innate immune activation by self-DNA during open mitosis — linking CDK1 to innate immunity regulation.

    Evidence In vitro kinase assay, cGAS S305A mutagenesis, cGAMP synthesis assay, PP1 dephosphorylation at mitotic exit

    PMID:32351706

    Open questions at the time
    • Whether CDK1-cGAS axis operates in non-dividing cells under pathological conditions is unexplored
    • Relative contribution of CDK1 versus other mitotic kinases to cGAS suppression is unclear
  11. 2022 High

    Discovery of a compartmentalized, Wee1-maintained inactive CDK1 pool on the mitotic spindle required for microtubule growth revealed that spatial inhibition of CDK1 within mitosis is essential for proper spindle assembly.

    Evidence Phospho-CDK1 immunofluorescence on spindle, Wee1/Cdc25 co-localization, live-cell spindle assembly phenotype

    PMID:35081344

    Open questions at the time
    • Molecular identity of spindle-associated CDK1 substrates that must remain unphosphorylated is unknown
    • How Wee1 is retained on spindle microtubules is not defined
  12. 2022 High

    S6K1 phosphorylation of CDK1 at S39 provided the first direct regulatory input from the mTORC1 pathway onto CDK1 activity, linking nutrient/growth signaling to cell cycle control and homologous recombination.

    Evidence In vitro S6K1 kinase assay on CDK1, S39A mutagenesis, HR reporter assay, cell cycle analysis

    PMID:36189922

    Open questions at the time
    • Whether S39 phosphorylation is constitutive or stimulus-specific in vivo is not characterized
    • Structural basis for how S39 phosphorylation inhibits CDK1 is unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • A unified quantitative model integrating CDK1's spatial compartmentalization, substrate-ordering thresholds, and non-catalytic scaffolding functions across the cell cycle remains to be constructed.
  • No structural model of CDK1 in complex with full-length cyclin B and substrate at atomic resolution
  • Temporal ordering of substrate phosphorylation events by rising CDK1 activity has not been measured genome-wide in human cells
  • Conservation of kinase-independent transcriptional functions in mammals is untested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 16 GO:0016740 transferase activity 5
Localization
GO:0005634 nucleus 4 GO:0005694 chromosome 2 GO:0005739 mitochondrion 2 GO:0005815 microtubule organizing center 2 GO:0005829 cytosol 2 GO:0005856 cytoskeleton 1
Pathway
R-HSA-1640170 Cell Cycle 11 R-HSA-162582 Signal Transduction 4 R-HSA-69306 DNA Replication 3 R-HSA-73894 DNA Repair 3 R-HSA-392499 Metabolism of proteins 2 R-HSA-168256 Immune System 1 R-HSA-5357801 Programmed Cell Death 1
Partners
CCNA2CCNB1CDC25CCKS1BMAD1L1MYT1PSMD12WEE1
Complex memberships
CDK1-Cks1Cyclin A-CDK1Cyclin B1-CDK1

Evidence

Reading pass · 62 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1987 A human homologue of the fission yeast cdc2 gene was cloned by complementation of a cdc2 mutant in fission yeast, establishing that the cell cycle control mechanism is conserved between yeast and humans. Functional complementation of S. pombe cdc2 mutant with human cDNA library Nature High 3553962
1988 Human p34cdc2 is phosphorylated at multiple sites, associates with p62 (cyclin B), and its kinase activity is cell cycle-regulated, peaking during mitotic metaphase; the complex is inactive in early G1 and maximally active at G2/M. Cell fractionation, immunoprecipitation, in vitro kinase assay, cell synchronization Cell High 3289755
1989 Cdc2 protein kinase forms separate complexes with cyclin A and cyclin B; these complexes display cell cycle-dependent histone H1 kinase activity, and cyclin proteolysis at the end of mitosis inactivates MPF. Immunoprecipitation with anti-cyclin A/B antibodies, p13suc1-Sepharose pulldown, in vitro histone H1 kinase assay Cell High 2538242
1989 Human cyclin B1 associates with p34cdc2 during G2/M phase, and this complex has histone H1 kinase activity; cyclin B1 is present in the cytoplasm during interphase and enters the nucleus at mitosis onset. Immunoprecipitation, in vitro kinase assay, cell cycle synchronization Cell High 2570636
1989 CDK1 (p34cdc2) is required for mitosis in mammalian cells: microinjection of anti-p34 antibodies blocked cell division without affecting DNA synthesis; p34 localizes to the nucleus during interphase and associates with centrosomes during mitosis. Microinjection of antibodies into rat fibroblasts, immunofluorescence localization Cell High 2541912
1990 Human p53 is phosphorylated by CDK1 (p60-cdc2 and cyclin B-cdc2) primarily at serine-315 in a cell cycle-dependent manner. In vitro kinase assay with purified p60-cdc2 and cyclin B-cdc2, phosphopeptide mapping, cell cycle fractionation Proceedings of the National Academy of Sciences of the United States of America High 2141171
1991 CDK1 (cdc2 kinase) phosphorylates smooth muscle caldesmon in vitro, reducing its binding to F-actin; phosphorylation sites are located in the COOH-terminal actin/calmodulin-binding domain, suggesting cdc2-mediated caldesmon phosphorylation contributes to microfilament disassembly during mitosis. In vitro kinase assay with mitotic HeLa cdc2 kinase, tryptic mapping, F-actin cosedimentation assay The Journal of biological chemistry High 2016282
1991 Cyclin A binds both CDK2 and CDC2 (CDK1), generating two distinct cyclin A kinase activities; anti-cyclin A antibody microinjection inhibits both DNA synthesis (S phase) and mitotic entry (G2/M), demonstrating CDK1-cyclin A has a role at two cell cycle points. Microinjection of anti-cyclin A antibodies, immunoprecipitation, in vitro kinase assay The EMBO journal High 1312467
1991 Cyclin A and cyclin B1 are differentially localized: cyclin A is predominantly nuclear from S phase onwards and associates with a 33-kDa PSTAIRE-containing protein (CDK1/CDK2), while cyclin B1 accumulates in the cytoplasm and only enters the nucleus at the beginning of mitosis, where it associates with p34cdc2. Immunofluorescence, cell fractionation, immunoprecipitation The Journal of cell biology High 1717476
1992 The retinoblastoma protein (pRB) physically associates with p34cdc2 or a closely related enzyme; the associated kinase activity phosphorylates pRB at most of its in vivo sites and is absent in G1 but appears in S phase, implicating CDK1 as a major regulator of pRB. Co-immunoprecipitation with anti-pRB antibodies, in vitro kinase assay on pRB substrate Molecular and cellular biology High 1545827
1992 CDK1 (cdc2) transcription is cell cycle-regulated: high in S and G2, low in G1; the retinoblastoma protein (Rb) represses cdc2 transcription via E2F binding sites in the 5' flanking region. Nuclear run-on assays, CAT reporter gene assays, deletion analysis of promoter elements The EMBO journal High 1582409
1992 CDK1 protein levels are maintained at a constant steady state through coordinated synthesis and degradation: transcription is activated at G1/S (12-fold), mRNA is rapidly degraded after mitosis, translation is shut off at mitotic onset, and >75% of new cdc2 complexes with cyclin. Metabolic labeling, pulse-chase, nuclear run-on, cell cycle synchronization, immunoprecipitation Proceedings of the National Academy of Sciences of the United States of America High 1372997
1994 CDK1 (cdc2 kinase) phosphorylates vimentin at Ser55 upon mitotic entry; a phospho-specific monoclonal antibody (4A4) confirmed that all cytoplasmic vimentin Ser55 residues are phosphorylated at mitotic entry and dephosphorylated during cytokinesis; the degree of vimentin filament disassembly correlated with mitotic cdc2 kinase activity. Monoclonal antibody 4A4 (phospho-Ser55 specific), Western blot, immunofluorescence, immunoelectron microscopy, Mono-Q fractionation, in vitro kinase assay The Journal of biological chemistry High 7983050
1995 Wee1 kinase phosphorylates CDK1 (Cdc2) at T14 in fission yeast; T14 phosphorylation plays an inhibitory role in Cdc2 activation and requires prior phosphorylation at Y15; Mik1 and Chk1 cannot drive T14 phosphorylation in vivo. In vivo phosphorylation analysis, chromosomal replacement with T14A/T14S cdc2 mutants, genetic epistasis Molecular biology of the cell High 7626804
1995 HIV-1 Vpr arrests cells in G2 by preventing activation of the p34cdc2/cyclin B complex, maintaining CDK1 in a phosphorylated, inactive state; expression of constitutively active CDK1 relieves the Vpr-induced G2 arrest. Histone H1 kinase assay of immunoprecipitated CDK1/cyclin B complexes, constitutively active CDK1 rescue experiment, flow cytometry Journal of virology High 7474080
1995 CDK1 (p34cdc2) phosphorylates human Eg5 kinesin at Thr-927 specifically during mitosis; mutation of Thr-927 to non-phosphorylatable residues prevents HsEg5 from binding to centrosomes, demonstrating that CDK1 phosphorylation controls spindle motor localization required for bipolar spindle formation. In vitro kinase assay with p34cdc2/cyclin B, site-directed mutagenesis (T927A), microinjection of anti-Eg5 antibodies, immunofluorescence Cell High 8548803
1996 Fission yeast Cdc2 kinase interacts with Orp2 (ORC2-like protein), which is required for DNA replication; Cdc2 thus controls DNA replication initiation by acting directly at chromosomal origins. Genetic interaction, two-hybrid, co-immunoprecipitation, in vitro kinase assay Nature High 8552194
1997 CDK1 inactivation is required for anaphase spindle dynamics and cytokinesis: expression of non-destructible cyclin B (preventing CDK1 inactivation) blocked chromosome decondensation, nuclear envelope reformation, and cytokinesis, and prevented F-actin/myosin II localization to the equator in normal rat kidney cells. Microinjection of non-destructible cyclin B mRNA into prometaphase cells, live imaging, rhodamine-tubulin injection, immunofluorescence The Journal of cell biology High 9230080
1997 Myt1 kinase phosphorylates CDK1 at Thr14, maintaining CDK1 in an inactive state during the G2-to-M phase transition; Wee1 phosphorylates Tyr15. In vitro kinase assay, site-directed mutagenesis, biochemical characterization of purified Myt1 Progress in cell cycle research High 9552418
1998 Fission yeast Cdc18 (Cdc6 homolog) interacts with Cdc2 in association with Cdc13 and Cig2 cyclins; Cdc2 phosphorylates Cdc18 in vitro and mutation of phosphorylation site T104A activates Cdc18 in a rereplication assay, establishing CDK1 as a negative regulator of replication re-initiation. Co-immunoprecipitation, in vitro kinase assay, site-directed mutagenesis, rereplication assay, genetic suppression Molecular biology of the cell High 9436991
1999 14-3-3σ sequesters CDK1 (cdc2) and cyclin B1 in the cytoplasm after DNA damage; loss of 14-3-3σ causes failure to maintain G2 arrest, allowing CDK1/cyclin B1 to enter the nucleus and leading to mitotic catastrophe. Somatic cell knockout of 14-3-3σ, immunofluorescence of cyclin B1/cdc2 localization, cell cycle analysis Nature High 10524633
2000 CDK1 (cdc2) kinase is required for optimal expression of a subset of herpes simplex virus late (γ2) genes; cdc2 specifically phosphorylates the second exon of ICP0 in vitro, and a dominant-negative cdc2 suppresses US11 accumulation. Dominant-negative cdc2 expression, in vitro kinase assay on viral polypeptides, immunoblot Proceedings of the National Academy of Sciences of the United States of America Medium 10995483
2001 Cdc2/cyclin B-cdc2 phosphorylates the dynein light intermediate chain (DLIC) at a conserved site; incubation of interphase membranes with cdc2-cyclinB1 kinase releases cytoplasmic dynein from the membrane, inhibiting organelle movement. In vitro kinase assay with purified Xenopus cdc2-GST-cyclinB1, mass spectrometry, membrane release assay The Journal of biological chemistry High 11278950
2001 Drosophila cdc2 is required for maintaining asymmetric localization of apical complex components (Inscuteable, Partner of Inscuteable) during mitosis in neural progenitors; Cdc2/B-type cyclin complexes are necessary for proper asymmetric division but not for initiation of apical complex formation. Genetic attenuation of Drosophila cdc2 function, immunofluorescence of asymmetric components, clonal analysis Nature High 11234018
2001 CDK1 (cdc2) forms stable molecular complexes with DNA topoisomerase II; this interaction stimulates topoisomerase II DNA binding and catalytic activity; the physical association is required for recruitment of cdc2 to the nucleus and is coupled to chromatin remodeling converting interphase nuclei into precondensation chromosomes. Co-immunoprecipitation, purified enzyme incubation with nuclei, pulse-field electrophoresis, chromatin remodeling assay FASEB journal Medium 11511510
2002 CDK1 (p34cdc2) kinase activity is required for a survival checkpoint after microtubule stabilization: elevated CDK1 activity induces survivin expression, and pharmacologic/genetic ablation of CDK1 after microtubule stabilization causes massive p53-independent apoptosis and suppresses tumor growth in mice. Pharmacologic CDK1 inhibition, genetic ablation, in vivo tumor model, apoptosis assays Cancer cell Medium 12150824
2003 CDK1 directly phosphorylates ~200 substrates in budding yeast identified by proteomic screening; several are phosphorylated in a CDK1-dependent manner in vivo, revealing a global regulatory strategy involving phosphorylation of other regulatory molecules and molecular machines. Analog-sensitive CDK1 allele with thiophosphate-ATP labeling in whole-cell extracts, proteomic library screen, in vivo validation Nature High 14574415
2003 CDK1 (cdc2) phosphorylates Disabled-2 (Dab2) during mitosis at multiple sites; cdc2 co-immunoprecipitates with Dab2 and purified cdc2 phosphorylates Dab2 in vitro; Dab2 phosphorylation promotes association with Pin1 peptidylprolyl isomerase. Co-immunoprecipitation, in vitro kinase assay with purified cdc2, roscovitine inhibition Oncogene Medium 12881709
2004 Drosophila Wee1 kinase regulates Cdk1 via phosphorylation of tyrosine 15, timing mitotic entry during syncytial blastoderm embryogenesis; loss of maternal dwee1 causes premature mitosis, spindle defects, chromosome condensation problems, and embryonic lethality. Genetic loss-of-function (maternal dwee1 mutants), in vitro kinase assay, immunofluorescence, Chk2 epistasis Current biology High 15589158
2005 CDK1 (Cdc28) and Cks1 are recruited to multiple coding regions and are required for efficient transcription of a significant subset of genes in budding yeast; this transcriptional role requires Cdc28/Cks1-mediated proteasome recruitment but is independent of Cdc28 kinase activity. ChIP, transcriptomics, kinase-dead allele analysis, proteasome recruitment assay Molecular cell Medium 15629725
2005 CDK1 phosphorylates vimentin at Ser55 (priming), creating a docking site for Plk1; Plk1 then phosphorylates vimentin at Ser82, which is required for vimentin filament segregation during mitosis; CDK1-Ser55 phosphorylation thus regulates mitotic vimentin phosphorylation via Plk1 recruitment. In vitro kinase assay, direct binding assay (Plk1-PBD to phospho-Ser55 vimentin), Plk1 depletion, mutational analysis (Ser55A, Ser82A), immunofluorescence The Journal of cell biology High 16260496
2007 CDK1 has an unexpected role in controlling bud growth after bud emergence in budding yeast; G1 cyclin-Cdk1 complexes specifically phosphorylate multiple proteins associated with Cdc24 (GEF for Cdc42); a mutant Cdc24-associated protein that cannot undergo Cdk1-dependent phosphorylation causes bud growth defects. Proteomics (mass spectrometry), in vitro kinase assay, site-directed mutagenesis, genetic analysis Nature cell biology High 17417630
2007 Mouse embryos lacking all interphase CDKs (Cdk2, Cdk3, Cdk4, Cdk6) can develop to midgestation with CDK1 binding all cyclins and phosphorylating pRb; embryos fail to develop past morula/blastocyst without Cdk1, establishing CDK1 as the only essential cell cycle CDK. Compound CDK knockout mice, MEF proliferation assays, pRb phosphorylation analysis, E2F target gene expression Nature High 17700700
2007 CDK1 (cdc2) phosphorylation of caldesmon regulates Schwann cell migration; Cdc2 expression increases in injury-preconditioned Schwann cells, and dominant-negative Cdc2 or dominant-negative caldesmon reduces migration; wild-type Cdc2 overexpression promotes distal Schwann cell migration in sciatic nerve. Dominant-negative and wild-type Cdc2 adenoviral transduction, migration assays, roscovitine inhibition, in vivo sciatic nerve implantation Journal of cell science Medium 17200138
2008 CDK1 directly regulates vacuole inheritance in budding yeast by phosphorylating the myosin V adaptor Vac17; phosphorylation of Vac17 parallels cell cycle-dependent vacuole movement, and non-phosphorylatable Vac17 shows decreased interaction with Myo2 and impaired vacuole inheritance. In vitro CDK1 kinase assay, site-directed mutagenesis of Cdk1 phosphorylation sites in Vac17, co-immunoprecipitation (Vac17-Myo2), vacuole inheritance assays Developmental cell High 18804442
2010 CyclinB1-CDK1 is inactive in G2 and activated at a defined time before nuclear envelope breakdown; different levels of CyclinB1-CDK1 kinase activity trigger different mitotic events, coordinating the mitotic entry program. FRET biosensor specific for CyclinB1-Cdk1 in living human cells, cell synchronization Developmental cell High 20412769
2010 CDK1 and CDK2 phosphorylation sites in mitotic cells show near-complete occupancy (>full phosphorylation) for up-regulated sites, particularly for nuclear proteins and proteins regulating metabolic processes, as determined by quantitative phosphoproteomics. High-resolution mass spectrometry-based phosphoproteomics with stoichiometry measurements across cell cycle stages Science signaling High 20068231
2011 CDK1 phosphorylates Sororin during mitosis; phosphorylation of Cdk1 sites causes Sororin release from chromatin and cohesin, thereby reducing sister chromatid cohesion; hypophosphorylated Sororin increases cohesion and premature mitotic exit caused by sororin knockdown is abolished by Aurora kinase inhibition. Site-directed mutagenesis of CDK1 phosphorylation sites, DNA-cellulose pulldown, co-immunoprecipitation, ZM447439 (Aurora kinase inhibitor) treatment, flow cytometry Journal of cell science High 21878504
2011 CDK1-phosphorylated CUEDC2 promotes spindle checkpoint inactivation: Cdk1 phosphorylates CUEDC2 during mitosis; phospho-CUEDC2 binds Cdc20 and promotes Mad2 release from APC/C-Cdc20, activating APC/C; CUEDC2 overexpression causes premature APC/C activation, chromosome missegregation, and aneuploidy. In vitro kinase assay, co-immunoprecipitation, siRNA depletion, APC/C ubiquitination assay, chromosome segregation analysis Nature cell biology High 21743465
2012 CDK1/cyclin B phosphorylates Ubc9 (SUMO E2 conjugating enzyme) at serine 71, enhancing its SUMOylation activity and increasing Ubc9-SUMO1 thioester conjugate levels; CDK2/cyclin E, ERK1, ERK2, PKA, and JNK2 do not show this effect. In vitro kinase assay with multiple kinases, Ubc9 SUMOylation assay, site-directed mutagenesis (S71A), thioester conjugate detection PloS one High 22509284
2013 Aurora B and CDK1 cooperate to phosphorylate Sororin, destabilizing interactions between Sororin and Pds5, thereby releasing acetylated cohesin from chromosome arms in prophase/prometaphase; at centromeres, Sgo1-PP2A antagonizes Aurora B and CDK1 by dephosphorylating Sororin, maintaining cohesion until metaphase. In vitro kinase assay, co-immunoprecipitation (Sororin-Pds5), phospho-specific antibodies, PP2A dephosphorylation assay, RNAi depletion Proceedings of the National Academy of Sciences of the United States of America High 23901111
2013 Cdk1/Cdc5 pathway activates Mus81-Mms4 via phosphorylation to resolve DNA damage tolerance recombination intermediates before anaphase; premature activation of the Cdk1/Cdc5/Mus81 pathway via phosphomimetic Mms4 variants or S-phase checkpoint-deficient backgrounds causes crossover-associated chromosome translocations. Phosphomimetic mutations (Mms4), checkpoint mutant analysis, genetic epistasis, two-dimensional gel electrophoresis for intermediates The EMBO journal High 23531881
2015 CDK1 phosphorylates SIRT3 at Thr150/Ser159, enhancing its deacetylase activity in mitochondria; radiation induces CDK1 and SIRT3 relocalization to mitochondria; Thr150Ala/Ser159Ala SIRT3 mutant shows reduced mitochondrial protein deacetylation, MnSOD activity, and ATP generation, and reduces tumor radioresistance. In vitro kinase assay (cyclin B1-CDK1 on SIRT3), site-directed mutagenesis (T150A/S159A), mitochondrial fractionation, MnSOD activity assay, clonogenic assay, in vivo xenograft Molecular cancer therapeutics High 26141949
2015 CDK1 relocates to mitochondria upon radiation and phosphorylates complex I subunits, boosting mitochondrial ATP generation; cells expressing kinase-deficient mitochondrially-targeted CDK1 or phosphorylation-deficient complex I subunits show reduced mitochondrial ATP and impaired nuclear DNA repair. Mitochondrial fractionation, CDK1 phosphorylation-deficient mutant complex I subunits, kinase-dead mitochondrially-targeted CDK1, oxygen consumption measurement, ATP generation assay, DNA repair (γH2AX) assay Cell reports High 26670043
2015 CDK1 phosphorylation of RepoMan differentially regulates associated phosphatases PP1 and PP2A-B56: CDK1 phosphorylation reduces PP1 binding but facilitates PP2A-B56 recruitment; CDK1 inactivation in early anaphase reverses this switch, allowing PP1-RepoMan to accumulate, inactivate Aurora B, and initiate nuclear envelope reassembly. In vitro CDK1 kinase assay on RepoMan, co-immunoprecipitation (PP1/PP2A-B56 with RepoMan), phospho-mutant analysis, live cell imaging of mitotic exit Nature communications High 26674376
2016 CDK1 in complex with cyclin A2 promotes adhesion complex and actin cytoskeleton organization during interphase (G1→S transition increases adhesion complex area); a decrease in adhesion complex area in G2 is triggered by inhibitory phosphorylation of CDK1-cyclin complexes requiring elevated cyclin B1, establishing CDK1 inactivation as the trigger for adhesion remodeling before mitosis. Cell synchronization, CDK1 inhibition (RO-3306), cyclin B1 siRNA, total internal reflection fluorescence microscopy of adhesion complexes, live imaging The Journal of cell biology High 29930204
2016 CDK1 (metaphase Cdk1) phosphorylates fimbrin at Thr103 in vitro and in vivo; conformational simulations suggest phosphorylation stabilizes fimbrin's N-terminal domain; phospho-fimbrin modulates actin filament binding to regulate actin cable assembly and stability in budding yeast. In vitro kinase assay (metaphase cyclin-Cdk1 complex), mass spectrometry identification of Thr103 phosphorylation, T103A/T103D mutants, actin cable imaging, molecular dynamics simulation Nature communications High 27068241
2016 CDK1 phosphorylates Ajuba at Ser119 and Ser175 during G2/M phase; mitotic phosphorylation of Ajuba controls cell cycle regulator expression, promotes cell proliferation and anchorage-independent growth in vitro, and promotes tumorigenesis in vivo, independent of Hippo pathway effects. In vitro CDK1 kinase assay, in vivo phosphorylation during cell cycle, site-directed mutagenesis (S119A/S175A), proliferation assays, xenograft tumor assay The Journal of biological chemistry High 27226586
2017 CDK1 coordinates nuclear envelope disassembly/reassembly with the cell cycle: CDK1 activity controls Lamin A/C targeting to chromatin (clock-based model), while PLK1 negatively regulates NPC assembly and is required for NPC disassembly at mitotic entry. CDK1 and PLK1 inhibition, immunofluorescence of Lamin A/C and NPC components, live-cell imaging Oncotarget Medium 29487689
2018 Premature activation of CDK1 (CDK1AF knockin with T14A/Y15F) causes S phase arrest, γH2AX formation, premature activation of MUS81-SLX4 endonuclease complexes, and untimely chromosome condensation/nuclear lamina disassembly, leading to embryonic lethality; CDK1AF MEFs show chromosomal fragmentation partly independent of CDK2. Cdk1AF knockin mice, MEF analysis, γH2AX immunofluorescence, MUS81-SLX4 inhibition, chromosome condensation assay Oncogene High 30190546
2018 DNA replication restricts CDK1 and PLK1 activation: human cells unable to initiate DNA replication prematurely enter mitosis, and blocking DNA replication licensing/firing causes prompt CDK1 and PLK1 activation in S phase; CDK1 activation during replication causes severe replication stress. Double-degron system for protein depletion, CDK1/PLK1 activity reporters, DNA replication inhibitors, kinase inhibitors (CHK1, p38) Molecular cell High 30008317
2018 CDK1 interacts with the pluripotency transcription factor Sox2 and phosphorylates it, promoting Sox2 nuclear localization and transcriptional activity; CDK1 kinase activity is required for CDK1-driven tumor-initiating capacity in melanoma. Proteomic analysis (CDK1 interactome), co-immunoprecipitation (CDK1-Sox2), Sox2 phosphorylation analysis, CDK1 overexpression/inhibition, Sox2 knockout in CDK1-OE cells, in vitro/in vivo tumor-initiating assays Cancer research Medium 30297536
2019 CDK1 primes BUB1 and CENP-U for PLK1 recruitment to kinetochores by phosphorylating docking sites; BUB1 and CENP-U are the main PLK1 kinetochore receptors in mitosis, and CDK1-dependent priming creates a positive feedback loop for MPS1 recruitment and sustained spindle checkpoint arrest. Ectopic localization assay, in vitro reconstitution with purified proteins, kinetochore localization studies, co-immunoprecipitation, phospho-mutant analysis Molecular cell High 33248027
2019 MAD1 recruits CDK1-CCNB1 to unattached kinetochores in an MPS1-dependent manner via interactions with the first 100 amino acids of MAD1; CDK1-CCNB1 at kinetochores phosphorylates MPS1 and creates a positive feedback loop for spindle checkpoint signaling. Proteomic analysis of CCNB1 complexes, co-immunoprecipitation, kinetochore localization analysis (with/without microtubule attachment), phospho-MPS1 assays, MAD1 truncation analysis The Journal of cell biology High 30674583
2019 CDK1 and CDK2 phosphorylate NICD (Notch intracellular domain) to regulate its stability and promote its degradation; inhibiting CDK1 or CDK2 increases NICD levels in vitro and in vivo, delays segmentation clock gene oscillations, and increases somite size. In vitro CDK1 kinase assay on NICD, CDK1/CDK2 inhibitors in cell and embryo models, NICD phospho-mutant analysis, somite size measurement EMBO reports High 31267714
2020 CDK1-cyclin B complex phosphorylates cGAS at S305 (human) or S291 (mouse) upon mitotic entry, inhibiting cGAS ability to synthesize cGAMP in response to self-DNA; PP1 phosphatase dephosphorylates cGAS upon mitotic exit to restore DNA sensing capacity. In vitro kinase assay (CDK1-cyclin B on cGAS), site-directed mutagenesis (S305A), cGAMP synthesis assay, cell imaging of cGAS localization during mitosis, PP1 dephosphorylation assay Cell discovery High 32351706
2020 CDK1 is a positive regulator of global translation (extramitotic function): CDK1 controls eIF2α, 4EBP, and S6K1 signaling pathways; CDK1 has a particularly strong effect on 5'TOP mRNA translation through LARP1 phosphorylation. Kinase/phosphatase screen, stress granule formation assay, cell synchronization, Ribo-Seq, LARP1 phosphorylation analysis by mass spectrometry The Journal of cell biology High 32040547
2021 USP7 limits CDK1 activity throughout the cell cycle by supporting active PP2A localization in the cytoplasm; USP7 inhibition causes widespread CDK1 activation and increased phosphorylation of CDK1 targets leading to DNA damage and cell toxicity; this toxicity is alleviated by lowering CDK1 activity or activating PP2A. USP7 inhibitors, phosphoproteomics (CDK1 target phosphorylation), PP2A co-immunoprecipitation with USP7, CDK1 activity rescue experiments The EMBO journal High 33856059
2022 CDK1 activity is compartmentalized during mitosis: a spindle-associated CDK1 fraction escapes Cdc25 activation and remains phosphorylated (inactive, i-Cdk1) through association with Wee1 while excluding Cdc25; this local CDK1 inhibition is required for spindle microtubule growth and spindle assembly. Immunofluorescence of phospho-CDK1 (i-Cdk1) on spindle, biochemical fractionation, live-cell imaging, Wee1/Cdc25 co-localization on spindle, loss/restoration of i-Cdk1 with defined spindle assembly phenotype Cell reports High 35081344
2022 S6K1 phosphorylates CDK1 at serine 39, causing G2/M cell cycle arrest and enabling homologous recombination DNA repair; this represents a direct regulatory input from mTORC1-S6K1 signaling onto CDK1 activity. In vitro kinase assay (S6K1 on CDK1), site-directed mutagenesis (S39A), cell cycle analysis, DNA repair assays (HR reporter) eLife High 36189922
2023 CDK1 phosphorylates USP29, activating its deubiquitinase activity toward TWIST1; CDK1-mediated USP29 phosphorylation is required for TWIST1 stabilization and TWIST1-driven EMT and cancer stem cell functions in triple-negative breast cancer. In vitro CDK1 kinase assay on USP29, deubiquitinase activity assay, co-immunoprecipitation (CDK1-USP29, USP29-TWIST1), CDK1 inhibition/knockout, phospho-mutant analysis Advanced science High 36782089
2025 PSMD12 interacts with CDK1, preventing its ubiquitin-mediated degradation through deubiquitination, thereby stabilizing CDK1 protein and promoting HCC cell cycle progression and cancer progression. Co-immunoprecipitation (PSMD12-CDK1), ubiquitination assay, PSMD12 knockdown/overexpression, cell proliferation and cycle analysis Frontiers in immunology Medium 40534847

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
1993 Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2. Cell 1367 8242750
1992 Cyclin A is required at two points in the human cell cycle. The EMBO journal 1318 1312467
2009 Defining the human deubiquitinating enzyme interaction landscape. Cell 1282 19615732
2010 Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Science signaling 1251 20068231
2016 ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure. Cell 1233 26777405
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2005 The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription. Molecular cell 1075 16109376
1992 Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle. Science (New York, N.Y.) 1040 1388288
2001 Nerve growth factor signaling, neuroprotection, and neural repair. Annual review of neuroscience 1029 11520933
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
1987 Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature 991 3553962
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2005 Nucleolar proteome dynamics. Nature 934 15635413
2004 Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nature biotechnology 916 15592455
1989 Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2. Cell 900 2570636
1992 A family of human cdc2-related protein kinases. The EMBO journal 891 1639063
2007 Cdk1 is sufficient to drive the mammalian cell cycle. Nature 861 17700700
2000 DNA cloning using in vitro site-specific recombination. Genome research 815 11076863
1995 Human immunodeficiency virus type 1 viral protein R (Vpr) arrests cells in the G2 phase of the cell cycle by inhibiting p34cdc2 activity. Journal of virology 809 7474080
1995 Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell 800 8548803
1991 Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport. The Journal of cell biology 798 1717476
1988 Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement. Cell 775 3289755
1999 14-3-3Sigma is required to prevent mitotic catastrophe after DNA damage. Nature 773 10524633
2003 Targets of the cyclin-dependent kinase Cdk1. Nature 751 14574415
2014 Partial inhibition of Cdk1 in G 2 phase overrides the SAC and decouples mitotic events. Cell cycle (Georgetown, Tex.) 737 24626186
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2010 Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis. Developmental cell 699 20412769
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
1989 Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF. Cell 627 2538242
1995 Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6. Molecular and cellular biology 618 7739547
1990 Human p53 is phosphorylated by p60-cdc2 and cyclin B-cdc2. Proceedings of the National Academy of Sciences of the United States of America 449 2141171
1989 The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells. Cell 412 2541912
1992 Cell cycle regulation of the human cdc2 gene. The EMBO journal 406 1582409
2010 An overview of Cdk1-controlled targets and processes. Cell division 326 20465793
2002 A p34(cdc2) survival checkpoint in cancer. Cancer cell 273 12150824
1995 Neuronal cdc2-like kinase. Trends in biochemical sciences 182 7878742
2003 Cyclin B1 and CDK1: nuclear localization and upstream regulators. Progress in cell cycle research 163 14593728
1997 CDK1 inactivation regulates anaphase spindle dynamics and cytokinesis in vivo. The Journal of cell biology 143 9230080
2023 Targeting CDK1 in cancer: mechanisms and implications. NPJ precision oncology 132 37311884
2013 Aurora B and Cdk1 mediate Wapl activation and release of acetylated cohesin from chromosomes by phosphorylating Sororin. Proceedings of the National Academy of Sciences of the United States of America 129 23901111
1992 The retinoblastoma protein physically associates with the human cdc2 kinase. Molecular and cellular biology 125 1545827
1996 Interaction of Cdc2 and Cdc18 with a fission yeast ORC2-like protein. Nature 123 8552194
2005 Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis. The Journal of cell biology 120 16260496
2007 Cdk1 coordinates cell-surface growth with the cell cycle. Nature cell biology 119 17417630
1994 Visualization and function of vimentin phosphorylation by cdc2 kinase during mitosis. The Journal of biological chemistry 119 7983050
1992 Coordinated synthesis and degradation of cdc2 in the mammalian cell cycle. Proceedings of the National Academy of Sciences of the United States of America 119 1372997
2018 Cell adhesion is regulated by CDK1 during the cell cycle. The Journal of cell biology 117 29930204
2013 Premature Cdk1/Cdc5/Mus81 pathway activation induces aberrant replication and deleterious crossover. The EMBO journal 114 23531881
2020 Phosphorylation of cGAS by CDK1 impairs self-DNA sensing in mitosis. Cell discovery 111 32351706
2018 CDK1 Interacts with Sox2 and Promotes Tumor Initiation in Human Melanoma. Cancer research 105 30297536
1991 The substrates of the cdc2 kinase. Seminars in cell biology 101 1842344
2018 DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation. Molecular cell 99 30008317
2001 cdc2 links the Drosophila cell cycle and asymmetric division machineries. Nature 93 11234018
2022 Ablation of cDC2 development by triple mutations within the Zeb2 enhancer. Nature 91 35732734
1995 The Wee1 protein kinase regulates T14 phosphorylation of fission yeast Cdc2. Molecular biology of the cell 89 7626804
2020 BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis. Molecular cell 88 33248027
2015 CDK1-Mediated SIRT3 Activation Enhances Mitochondrial Function and Tumor Radioresistance. Molecular cancer therapeutics 84 26141949
2015 CDK1 Enhances Mitochondrial Bioenergetics for Radiation-Induced DNA Repair. Cell reports 81 26670043
2020 CDK1 couples proliferation with protein synthesis. The Journal of cell biology 80 32040547
2014 Control of Cdc28 CDK1 by a stress-induced lncRNA. Molecular cell 77 24508389
2005 Cell cycle sibling rivalry: Cdc2 vs. Cdk2. Cell cycle (Georgetown, Tex.) 77 16258277
2001 Differential regulation of Cdc2 and Cdk2 by RINGO and cyclins. The Journal of biological chemistry 75 11461916
1998 Negative regulation of Cdc18 DNA replication protein by Cdc2. Molecular biology of the cell 72 9436991
2006 Discovery and evaluation of dual CDK1 and CDK2 inhibitors. Cancer research 71 16618755
2019 MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling. The Journal of cell biology 70 30674583
2011 Cdk1-phosphorylated CUEDC2 promotes spindle checkpoint inactivation and chromosomal instability. Nature cell biology 70 21743465
2024 Distinct ontogenetic lineages dictate cDC2 heterogeneity. Nature immunology 67 38351322
2004 Drosophila Wee1 kinase regulates Cdk1 and mitotic entry during embryogenesis. Current biology : CB 67 15589158
1996 Regulation of Cdc2 activity by phosphorylation at T14/Y15. Progress in cell cycle research 67 9552387
2015 Cdk1 orders mitotic events through coordination of a chromosome-associated phosphatase switch. Nature communications 64 26674376
1995 The role of cdc2 and other genes in meiosis in Schizosaccharomyces pombe. Genetics 64 7498766
1995 A family of trypanosome cdc2-related protein kinases. Gene 64 7557404
2011 Cdc2: a monopotent or pluripotent CDK? Cell proliferation 63 21535261
2011 Regulation of sororin by Cdk1-mediated phosphorylation. Journal of cell science 61 21878504
1991 Phosphorylation of caldesmon by cdc2 kinase. The Journal of biological chemistry 61 2016282
1997 Myt1: a Wee1-type kinase that phosphorylates Cdc2 on residue Thr14. Progress in cell cycle research 56 9552418
1995 Coexpression of cdk2/cdc2 and retinoblastoma gene products in colorectal cancer. British journal of cancer 56 7779716
2007 Cdc2-mediated Schwann cell migration during peripheral nerve regeneration. Journal of cell science 54 17200138
2006 Necdin downregulates CDC2 expression to attenuate neuronal apoptosis. The Journal of neuroscience : the official journal of the Society for Neuroscience 53 17108174
2000 The role of cdc2 in the expression of herpes simplex virus genes. Proceedings of the National Academy of Sciences of the United States of America 53 10995483
1998 CDK9 (PITALRE): a multifunctional cdc2-related kinase. Journal of cellular physiology 52 10092203
2018 Premature activation of Cdk1 leads to mitotic events in S phase and embryonic lethality. Oncogene 51 30190546
2012 Phosphorylation of Ubc9 by Cdk1 enhances SUMOylation activity. PloS one 50 22509284
2005 A kinase-independent function of Cks1 and Cdk1 in regulation of transcription. Molecular cell 49 15629725
1995 Cell cycle. The NIMA kinase joins forces with Cdc2. Current biology : CB 46 8548283
1999 Learning induces a CDC2-related protein kinase, KKIAMRE. The Journal of neuroscience : the official journal of the Society for Neuroscience 45 10531455
1992 cdc2-like kinase is associated with the retinoblastoma protein. Oncogene 44 1594240
2016 Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis. The Journal of biological chemistry 43 27226586
2017 Residual Cdk1/2 activity after DNA damage promotes senescence. Aging cell 41 28345297
2014 Phase I study of the novel Cdc2/CDK1 and AKT inhibitor terameprocol in patients with advanced leukemias. Investigational new drugs 41 25523151
2001 Phosphorylation by cdc2-CyclinB1 kinase releases cytoplasmic dynein from membranes. The Journal of biological chemistry 41 11278950
1991 A cdc2-like kinase phosphorylates histone H1 in the amitotic macronucleus of Tetrahymena. The EMBO journal 40 2065655
2025 PSMD12 promotes hepatocellular carcinoma progression by stabilizing CDK1. Frontiers in immunology 39 40534847
2023 Phosphorylation of USP29 by CDK1 Governs TWIST1 Stability and Oncogenic Functions. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 39 36782089
2008 The cyclin-dependent kinase Cdk1 directly regulates vacuole inheritance. Developmental cell 39 18804442
2001 From progesterone to active Cdc2 in Xenopus oocytes: a puzzling signalling pathway. Biology of the cell 38 11730320
2013 Dinaciclib (SCH727965) inhibits the unfolded protein response through a CDK1- and 5-dependent mechanism. Molecular cancer therapeutics 37 24362465
2021 Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells. Cell death discovery 36 33863878
2002 Multiple Cdk1 inhibitory kinases regulate the cell cycle during development. Developmental biology 35 12217326
2017 CDK1 and PLK1 coordinate the disassembly and reassembly of the nuclear envelope in vertebrate mitosis. Oncotarget 34 29487689
2022 An Integrative Human Pan-Cancer Analysis of Cyclin-Dependent Kinase 1 (CDK1). Cancers 33 35681641
2016 Mitotic entry: The interplay between Cdk1, Plk1 and Bora. Cell cycle (Georgetown, Tex.) 33 27831827
2009 Cdc28/Cdk1 positively and negatively affects genome stability in S. cerevisiae. The Journal of cell biology 33 19398760
2003 Cell cycle-dependent phosphorylation of Disabled-2 by cdc2. Oncogene 33 12881709
2017 CDK1 interacts with iASPP to regulate colorectal cancer cell proliferation through p53 pathway. Oncotarget 31 29069733
2022 NCAPD2 promotes breast cancer progression through E2F1 transcriptional regulation of CDK1. Cancer science 30 35348268
2021 USP7 limits CDK1 activity throughout the cell cycle. The EMBO journal 30 33856059
2019 CDK1 and CDK2 regulate NICD1 turnover and the periodicity of the segmentation clock. EMBO reports 30 31267714
2016 Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast. Nature communications 30 27068241
2004 Abi enhances Abl-mediated CDC2 phosphorylation and inactivation. Journal of biomedical science 30 15591787
2016 Recurrent CDK1 overexpression in laryngeal squamous cell carcinoma. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 29 26912061
1996 Neuronal Cdc2-like kinase: from cell cycle to neuronal function. Biochemistry and cell biology = Biochimie et biologie cellulaire 29 8960348
1991 Regulation of cdc2 activity in Schizosaccharomyces pombe: the role of phosphorylation. Seminars in cell biology 29 1842338
2016 LAR protein tyrosine phosphatase regulates focal adhesions through CDK1. Journal of cell science 27 27352860
2003 Chemical and biological profile of dual Cdk1 and Cdk2 inhibitors. Current medicinal chemistry. Anti-cancer agents 27 12678910
2001 Recruitment of cdc2 kinase by DNA topoisomerase II is coupled to chromatin remodeling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25 11511510
2000 Temperature dependent expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis. Cell research 25 11191351
1997 MAP and cdc2 kinase activities at germinal vesicle breakdown in Chaetopterus. Developmental biology 25 9398433
1998 Entry into mitosis without Cdc2 kinase activation. Journal of cell science 24 9788881
1995 Interaction of cdc2 and rum1 regulates Start and S-phase in fission yeast. Journal of cell science 24 7593289
2021 Overexpressed DEPDC1B contributes to the progression of hepatocellular carcinoma by CDK1. Aging 23 34032605
2014 Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PloS one 23 24551245
2011 Ribosomal protein S3 is phosphorylated by Cdk1/cdc2 during G2/M phase. BMB reports 23 21871177
2015 Create, activate, destroy, repeat: Cdk1 controls proliferation by limiting transcription factor activity. Current genetics 22 26590602
2003 Beacon interacts with cdc2/cdc28-like kinases. Biochemical and biophysical research communications 22 12705895
2022 S6K1 phosphorylates Cdk1 and MSH6 to regulate DNA repair. eLife 21 36189922
2022 Compartmentalized control of Cdk1 drives mitotic spindle assembly. Cell reports 20 35081344