Affinage

CCNB1

G2/mitotic-specific cyclin-B1 · UniProt P14635

Length
433 aa
Mass
48.3 kDa
Annotated
2026-06-09
99 papers in source corpus 30 papers cited in narrative 30 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Cyclin B1 (CCNB1) is the regulatory subunit of the CDK1-CCNB1 mitotic kinase that drives the G2/M transition in mitotic and meiotic cell cycles, and its activity is gated by exquisitely tight control of its abundance, localization, and timing (PMID:30674583, PMID:28808066). Beyond simply activating CDK1, CDK1-CCNB1 is an integral component of the spindle assembly checkpoint: it localizes to unattached kinetochores in an MPS1-dependent manner through direct interaction with the N-terminal 100 residues of MAD1, and phosphorylates MPS1 at Ser281 to sustain its kinetochore binding, creating a positive feedback loop that maintains checkpoint arrest until CCNB1 levels fall below the threshold that fully activates the counteracting PP2A-B55 phosphatase to permit mitotic exit (PMID:30674583, PMID:30674582). CDK1-CCNB1 also inhibits separase by forming an inhibitory CDK1-CCNB1-CKS1 complex that blocks cohesin cleavage (PMID:34616878). Nuclear translocation of CCNB1 — a prerequisite for mitotic entry — is promoted by direct MAPK1 (ERK2) phosphorylation at Ser128 (PMID:41905453). CCNB1 abundance is set transcriptionally by activators that bind its promoter, including FOXM1, ISL1, STOX1A, and E2F1 recruited by AURKA (PMID:29705704, PMID:27183908, PMID:22253775, PMID:38655290), and is further controlled post-transcriptionally and post-translationally: m7G-WDR4/EIF2A and METTL3-m6A pathways enhance CCNB1 mRNA stability and translation (PMID:34244479, PMID:39954139); deubiquitinases USP22 (itself activated by CDK1 phosphorylation) and UCHL1 stabilize the protein by antagonizing proteasomal degradation (PMID:27030811, PMID:38858680); and degradation proceeds through APC/C-CDC20-coupled and UBA52-APC11 ubiquitylation (PMID:27030811, PMID:31814919). In oocyte meiosis, alternative polyadenylation generates Ccnb1 mRNAs with short or long 3' UTRs whose differential translation times meiotic re-entry versus the MI/MII transition, with long-3'UTR translation requiring ERK1/2-driven CPEB1 degradation (PMID:28808066, PMID:38048302, PMID:33282880). CCNB1 function is physiologically required for germ cell proliferation, as conditional knockout in mouse male germ cells causes germ cell depletion and sterility (PMID:29072697).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2012 Medium

    Establishing that CCNB1 is a direct transcriptional target answered how mitotic entry is coupled to cell-cycle gene programs.

    Evidence ChIP and STOX1A gain/loss-of-function with CCNB1 readout in cultured cells

    PMID:22253775

    Open questions at the time
    • Does not establish whether STOX1A acts directly versus through cofactors
    • No structural detail of the promoter element
  2. 2015 High

    Identifying USP22 as a CDK1-activated deubiquitinase of CCNB1 revealed that cyclin stability is reinforced by a kinase-coupled positive loop, not solely by APC/C-driven destruction.

    Evidence Co-IP, ubiquitination assays, proteasome inhibitor rescue, and CDK1-phosphorylation validation in human cells

    PMID:27030811

    Open questions at the time
    • Quantitative contribution of USP22 versus APC/C to net CCNB1 turnover unresolved
    • Site of CDK1 phosphorylation on USP22 not detailed
  3. 2016 Medium

    Multiple transcription factors (ISL1, later FOXM1, E2F1) were shown to converge on the CCNB1 promoter, explaining how diverse oncogenic signals raise CCNB1 to force G2/M progression.

    Evidence ChIP and overexpression/knockdown with CCNB1 readouts across cancer cell models

    PMID:27183908 PMID:29705704 PMID:38655290

    Open questions at the time
    • Hierarchy or combinatorial logic among these activators not defined
    • Context-dependence across tissues unclear
  4. 2017 High

    Dissecting 3'UTR-dependent translational timing answered how a single mRNA can be deployed at distinct meiotic stages without new transcription.

    Evidence In vivo translation reporters with 3'UTR/CPE mutagenesis in mouse oocytes

    PMID:28808066

    Open questions at the time
    • Identity of all trans-acting factors reading the cis-elements incomplete
    • Does not address mitotic somatic translational control
  5. 2017 High

    Conditional knockout established the in vivo physiological requirement for CCNB1 in germ cell proliferation rather than meiosis itself.

    Evidence Germ-cell conditional knockout mice with fertility, apoptosis, and Lin28a/let-7 readouts

    PMID:29072697

    Open questions at the time
    • Mechanism linking CCNB1 loss to Lin28a/let-7 change unresolved
    • Redundancy with CCNB2 not tested
  6. 2019 High

    Demonstrating MAD1-dependent recruitment of CDK1-CCNB1 to unattached kinetochores and MPS1-S281 phosphorylation redefined CCNB1 as an integral checkpoint component, not merely the downstream kinase that the checkpoint protects.

    Evidence Reciprocal Co-IP, proteomics, phospho-mutant rescue, MPS1-Mis12 tethering, and live-cell imaging in human cells (companion studies)

    PMID:30674582 PMID:30674583

    Open questions at the time
    • Structural basis of the MAD1-CCNB1 interface not resolved
    • Quantitative threshold model of CCNB1 versus PP2A-B55 needs in vivo calibration
  7. 2019 Medium

    Defining UBA52-APC11 ubiquitylation of CCNB1 clarified the molecular steps of APC/C-coupled CCNB1 destruction controlling mitotic exit.

    Evidence Co-IP and epistatic knockdown/overexpression with cell-cycle and xenograft readouts in NSCLC cells

    PMID:31814919

    Open questions at the time
    • Direct ligase-substrate reconstitution not shown
    • Relationship to canonical CDC20-APC/C unclear
  8. 2021 Medium

    Identifying m7G-WDR4/EIF2A control of CCNB1 mRNA and downstream PI3K/AKT and p53 effects extended CCNB1 regulation to translational layers and linked it to growth/survival signaling.

    Evidence RNA stability and translation reporters, RIP for EIF2A-CCNB1 mRNA, and Co-IP in cancer cells

    PMID:34244479

    Open questions at the time
    • Whether p53 ubiquitination is a direct CCNB1-driven effect is not established
    • Single-lab finding
  9. 2021 Medium

    Multiple meiotic regulators (Septin 4, Septin 9, SIN3A) were placed upstream of CCNB1 via APC/C-CDC20 or transcriptional control, mapping how oocyte and early embryonic cell-cycle transitions tune CCNB1 levels.

    Evidence siRNA knockdown with mRNA-rescue epistasis, live imaging, and IP in oocyte/embryo models

    PMID:33692994 PMID:34189751 PMID:36354207

    Open questions at the time
    • Direct biochemical links between these regulators and the APC/C machinery incomplete
    • Generalizability beyond the specific reproductive contexts unclear
  10. 2021 Medium

    Structural analysis of the separase-CDK1-CCNB1-CKS1 complex revealed how CCNB1 contributes to a non-kinase inhibitory mechanism restraining cohesin cleavage in parallel to securin.

    Evidence Structural determination of human separase bound to the CDK1-CCNB1-CKS1 complex (commentary on primary structures)

    PMID:34616878

    Open questions at the time
    • Primary structural data not in this corpus
    • Temporal coordination with securin inhibition in cells not addressed here
  11. 2023 Medium

    Discovery of SQLE and UCHL1 as CCNB1-stabilizing interactors broadened the deubiquitination/stability network and connected CCNB1 control to lipid-metabolic and reproductive contexts.

    Evidence Co-IP, ubiquitination and cycloheximide-chase half-life assays with functional readouts

    PMID:37839786 PMID:38858680

    Open questions at the time
    • Whether SQLE/UCHL1 act directly as enzymes versus scaffolds incompletely resolved
    • Cell-cycle phase specificity of these interactions unclear
  12. 2025 Medium

    METTL3-mediated m6A modification was shown to stabilize CCNB1 mRNA, adding an epitranscriptomic layer transferable via exosomes to drive proliferation.

    Evidence MeRIP, actinomycin D stability assay, and METTL3/CCNB1 knockdown-rescue in fibroblasts

    PMID:39954139

    Open questions at the time
    • Reader proteins decoding the m6A mark on CCNB1 not identified
    • Single-lab finding
  13. 2026 High

    Direct MAPK1 phosphorylation of CCNB1-Ser128 established a signaling input that gates CCNB1 nuclear translocation and the G2-to-prophase transition.

    Evidence TurboID-MS, in vitro kinase assay, S128A/D mutants, fractionation, and xenograft in TNBC cells

    PMID:41905453

    Open questions at the time
    • Interplay with other reported translocation regulators (e.g., SIAH1) not tested
    • Whether Ser128 phosphorylation alters CDK1 activation is not addressed

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the many transcriptional, translational, and stability inputs are quantitatively integrated to produce the precise CCNB1 threshold that governs checkpoint silencing and mitotic exit remains unresolved.
  • No unified quantitative model linking CCNB1 abundance to PP2A-B55 activation in vivo
  • Relative contributions of competing stability regulators across cell types unknown
  • Structural basis of kinetochore recruitment interfaces undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 3 GO:0140096 catalytic activity, acting on a protein 1
Localization
GO:0005634 nucleus 2 GO:0005829 cytosol 2
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-1474165 Reproduction 3
Partners
CDK1CKS1MAD1MAPK1MPS1SQLEUCHL1USP22
Complex memberships
CDK1-CCNB1 (MPF)CDK1-CCNB1-CKS1 separase-inhibitory complex

Evidence

Reading pass · 30 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2019 CDK1-CCNB1 localizes to unattached kinetochores (lost upon microtubule attachment) and is recruited there in an MPS1-dependent fashion through direct interaction with the first 100 amino acids of MAD1. This pool of CDK1-CCNB1 creates a positive feedback loop for timely MPS1 kinetochore recruitment and sustained spindle checkpoint arrest, making CDK1-CCNB1 an integral component of the spindle checkpoint. Complementary proteomics, reciprocal Co-IP, cell biological imaging, and genetic rescue experiments The Journal of cell biology High 30674583
2019 CDK1-CCNB1 phosphorylates MPS1 at S281 to enable its kinetochore binding during mitotic entry; a counteracting phosphatase PP2A-B55 (itself negatively regulated by CDK1-CCNB1) dephosphorylates S281 to promote mitotic exit. This creates a spindle checkpoint-permissive state that persists until CCNB1 concentration falls below a threshold that fully activates PP2A-B55. Phospho-specific mutant analysis (MPS1-S281A/D), tethering bypass experiments (MPS1-Mis12 fusion), quantitative live-cell imaging, and genetic epistasis The Journal of cell biology High 30674582
2015 USP22 is a deubiquitinase of CCNB1: it directly interacts with CCNB1, stabilizes it by antagonizing proteasome-mediated degradation in a cell-cycle-specific manner, and its deubiquitinating activity toward CCNB1 is enhanced by CDK1-mediated phosphorylation of USP22. APC/C-CDC20 targets USP22 for degradation during mitotic exit, thereby permitting CCNB1 degradation. Co-immunoprecipitation, ubiquitination assays, proteasome inhibitor rescue, cell-cycle fractionation, USP22 knockdown with CCNB1 protein-level readout Cell discovery High 27030811
2021 WDR4 (m7G methyltransferase) promotes CCNB1 mRNA stability and translation by enhancing binding of EIF2A to CCNB1 mRNA. CCNB1 in turn promotes PI3K/AKT phosphorylation and reduces p53 protein expression by promoting p53 ubiquitination. RNA stability assays, translation reporter assays, RIP (RNA immunoprecipitation) for EIF2A-CCNB1 mRNA interaction, Co-IP, WDR4 knockdown/overexpression with protein-level readouts Cell death & disease Medium 34244479
2017 The timing of Ccnb1 mRNA translation in mouse oocytes is determined by the presence of transcripts with distinct 3' UTRs generated by alternative polyadenylation: inclusion or exclusion of specific cis-acting elements directs early vs. late translational activation during meiotic maturation. In vivo translation reporter assays with 3'UTR variants, cytoplasmic polyadenylation element (CPE) mutation analysis, oocyte microinjection Genes & development High 28808066
2024 Alternative cleavage and polyadenylation (APA) generates Ccnb1 mRNA variants with short or long 3' UTRs; in vivo mutation of proximal (short) polyadenylation elements showed that short-3'UTR mRNA translation specifies the Cyclin B1 levels that set the timing of meiotic re-entry, whereas long-3'UTR mRNA translation drives Cyclin B1 accumulation during the MI/MII transition. CRISPR/gene editing to mutate polyadenylation signals in mice, in vivo loss-of-function experiments, mRNA variant quantification Nucleic acids research High 38048302
2020 ERK1/2 (MAPK) activation is required for translational activation of Ccnb1 long-3'UTR transcripts during meiotic resumption in mouse oocytes. CDK1 activity also contributes to Ccnb1 mRNA translational activation. ERK1/2-driven degradation (not just phosphorylation) of CPEB1 is the principal mechanism, whereas CDK1 only phosphorylates CPEB1 without triggering its degradation. MAPK inhibition/activation in GV-stage oocytes, CDK1 inhibitor/activator treatments, CPEB1 phosphorylation and degradation assays, Ccnb1-3'UTR translation reporters Frontiers in cell and developmental biology Medium 33282880
2018 FOXM1 directly binds the CCNB1 promoter and transcriptionally activates CCNB1 expression; CCNB1 is required for FOXM1-driven cell proliferation in hepatocellular carcinoma cells. ChIP (chromatin immunoprecipitation) demonstrating FOXM1 binding to CCNB1 promoter, FOXM1 knockdown with mRNA/protein-level readouts, CCNB1 rescue experiments Biochemical and biophysical research communications Medium 29705704
2016 ISL1 transcription factor directly binds conserved sites in the CCNB1 (and CCNB2, c-MYC) promoters/enhancers to activate their expression, promoting G2/M cell cycle transition in gastric cancer cells. ChIP demonstrating ISL1 binding to CCNB1 promoter/enhancer, ISL1 overexpression and knockdown with CCNB1 mRNA/protein-level readouts, cell cycle analysis Oncotarget Medium 27183908
2012 STOX1A transcription factor directly binds the CCNB1 promoter (shown by ChIP) and upregulates cyclin B1 expression, promoting mitotic entry. ChIP, STOX1A overexpression and knockdown with CCNB1 expression readout, cell cycle analysis PloS one Medium 22253775
2019 CCNB1 degradation by APC11 is mediated through UBA52-driven ubiquitylation of CCNB1; knockdown of APC11 leads to G2/M arrest even when UBA52 is overexpressed, placing APC11 downstream of UBA52 in regulating CCNB1 stability and cell cycle progression in NSCLC cells. Co-immunoprecipitation, shRNA knockdown and gene overexpression, cell cycle analysis, xenograft tumor assays American journal of translational research Medium 31814919
2021 Circ-CCNB1 acts as a sponge for miR-223, leading to elevated SIAH1 expression; SIAH1 in turn inhibits CCNB1 nuclear translocation. This circ-CCNB1/miR-223/SIAH1 axis suppresses trophoblast proliferation and invasion, establishing that CCNB1 nuclear translocation is regulated through SIAH1. RNA pull-down, dual luciferase reporter assay, siRNA knockdown, nuclear-cytoplasmic fractionation, Co-IP/immunofluorescence for CCNB1 localization Endocrinology Medium 35731831
2017 CCNB1 is required for normal proliferation of gonocytes and spermatogonia in mouse testes; conditional knockout of CCNB1 in male germ cells causes male sterility due to germ cell depletion and increased apoptosis, and also promotes spermatogonial differentiation by downregulating Lin28a and upregulating let-7 miRNA. Notably, ablation of CCNB1 in premeiotic cells (spermatocytes) did not affect meiosis or male fertility, suggesting CCNB1 is dispensable for meiosis in spermatocytes. Conditional knockout mice, immunofluorescence, TUNEL assay, Lin28a/let-7 expression analysis, fertility assays Cell death & disease High 29072697
2021 SIN3A regulates CCNB1 expression in porcine early embryos; SIN3A depletion causes a two-cell developmental block associated with dramatically reduced CCNB1 transcript levels, and injection of exogenous CCNB1 mRNA into SIN3A-depleted embryos partially rescues development past the two-cell stage, placing SIN3A upstream of CCNB1 in early embryonic cell cycle progression. siRNA knockdown, RNA-seq, mRNA microinjection rescue, immunofluorescence Frontiers in cell and developmental biology Medium 33692994
2021 Septin 4 regulates CCNB1 accumulation in mouse oocytes via APC/C-CDC20: depletion of Septin 4 increases CDC20 expression, reduces CCNB1 accumulation, and causes GV arrest; this arrest can be rescued by CCNB1 overexpression or CDC20 depletion, placing Septin 4 upstream of APC/C-CDC20-mediated CCNB1 stabilization at the meiotic G2/M transition. siRNA knockdown, mRNA microinjection rescue, immunoprecipitation, immunofluorescence, time-lapse live imaging, immunoblotting Journal of cellular physiology Medium 34189751
2022 Septin 9 depletion in mouse oocytes causes MI arrest with persistent SAC signaling (Mad1, BubR1 on kinetochores), failure of CDC20 upregulation and CCNB1 degradation, and disrupted kinetochore-microtubule connections; CDC20 mRNA microinjection partially rescues the arrest, establishing that Septin 9 promotes MI-AI transition through SAC/APC/C-CDC20-dependent CCNB1 degradation. siRNA knockdown, mRNA microinjection rescue, time-lapse live imaging, immunofluorescence for SAC proteins, immunoblotting for CDC20/CCNB1 Cell proliferation Medium 36354207
2021 Human separase is inhibited by CDK1-CCNB1-CKS1 complex; structural analysis defined molecular mechanisms by which this complex blocks separase's cohesin-cleavage activity, in parallel with inhibition by securin. Structural biology (cryo-EM/crystallography implied by 'structures') of human separase bound to CDK1-CCNB1-CKS1 complex Molecular & cellular oncology Medium 34616878
2023 SQLE (squalene epoxidase) directly interacts with CCNB1 and alleviates its ubiquitination modification, enhancing CCNB1 protein stability; this SQLE-CCNB1 interaction was demonstrated to promote breast cancer cell cycle progression and inhibit ferroptosis. Co-immunoprecipitation, immunofluorescence colocalization, ubiquitination assay, SQLE overexpression/knockdown with CCNB1 protein-level readout, xenograft Experimental cell research Medium 37839786
2024 UCHL1 (deubiquitinase) directly binds CCNB1, prolongs its half-life and inhibits its degradation, thereby promoting granulosa cell proliferation; the flavonoid isovitexin enhances UCHL1 enzymatic activity and downstream CCNB1 stabilization. Co-immunoprecipitation, half-life (cycloheximide chase) assays, UCHL1 overexpression/knockdown with CCNB1 protein-level readout, enzyme activity assay Journal of animal science and biotechnology Medium 38858680
2025 METTL3 mediates m6A modification of CCNB1 mRNA, upregulating its stability and expression; METTL3-modified ASC-exosomes transfer this activity to dermal fibroblasts, enhancing their proliferation and migration in a CCNB1-dependent manner. Methylated RNA immunoprecipitation (MeRIP), actinomycin D mRNA stability assay, qPCR, METTL3 overexpression/knockdown, CCNB1 knockdown rescue experiments Archives of dermatological research Medium 39954139
2024 AURKA interacts with transcription factor E2F1 and recruits it to the CCNB1 promoter to drive CCNB1 transcription; CCNB1 expression is required for AURKA-induced renal cell carcinoma progression. Co-immunoprecipitation (AURKA-E2F1 interaction), ChIP (E2F1 at CCNB1 promoter), luciferase reporter assay, AURKA/CCNB1 knockdown with functional rescue Heliyon Medium 38655290
2025 SNRPB regulates CCNB1 expression through FOXM1-mediated transcriptional activation: SNRPB interacts with FOXM1 (shown by Co-IP), and this complex drives CCNB1 promoter activity (shown by ChIP). CCNB1 knockdown reverses proliferative and tumorigenic effects of SNRPB overexpression in HCC. Co-immunoprecipitation (SNRPB-FOXM1), ChIP (FOXM1 at CCNB1 promoter), CCNB1 knockdown rescue in SNRPB-overexpressing cells, in vivo xenograft Journal of experimental & clinical cancer research Medium 40682115
2021 MEOX1 transcription factor binds the transcriptional initiation site of CCNB1 and suppresses its expression; CCNB1 overexpression rescues the proliferation inhibition caused by MEOX1 overexpression in NSCLC cells, placing MEOX1 upstream of CCNB1 in a suppressive axis. ChIP or EMSA (binding to CCNB1 transcription initiation site), CCNB1 rescue of MEOX1 overexpression phenotype, in vitro and in vivo functional assays Environmental toxicology Low 34837450
2026 MAPK1 (ERK2) directly phosphorylates CCNB1 at Ser128; this phosphorylation facilitates CCNB1 nuclear translocation and the G2-to-prophase transition. A phospho-mimetic CCNB1-S128D mutant partially rescues proliferation defects caused by MAPK1 knockdown in TNBC cells. TurboID proximity labeling + mass spectrometry to identify MAPK1-CCNB1 interaction, Co-IP, in vitro kinase assay, CCNB1-S128A/D mutants, nuclear-cytoplasmic fractionation, immunofluorescence, cell cycle analysis, xenograft Life sciences High 41905453
2025 HROB suppresses LUAD progression by interacting with ZC3HC1 and reducing its phosphorylation at Ser354; this de-phosphorylation event facilitates K27-linked ubiquitination of CCNB1, promoting its proteasomal degradation and impairing the G2-to-M phase transition. Co-IP (HROB-ZC3HC1 interaction), phospho-specific analysis of ZC3HC1-Ser354, ubiquitination assay for K27-linked CCNB1 ubiquitination, proteasomal degradation assay, cell cycle analysis Cancer science Medium 40654113
2024 GADD45G directly interacts with CDK1 and CCNB1 (shown by Co-IP), and negatively regulates CDK1/CCNB1 at transcript and protein levels; reduced GADD45G expression (due to promoter methylation) leads to invasive behavior in endometrial cancer cells through upregulated CDK1/CCNB1. Co-immunoprecipitation (GADD45G with CDK1 and CCNB1), methylation-specific PCR, qRT-PCR and western blot, functional invasion assays Journal of cancer research and therapeutics Low 39206984
2025 Luteolin directly binds CCNB1 (confirmed by molecular docking and surface plasmon resonance), disrupts the CCNB1/CDK1 complex, induces G2/M arrest, and suppresses pancreatic cancer growth in vitro and in vivo. Surface plasmon resonance (direct binding assay), molecular docking, CCNB1/CDK1 co-immunoprecipitation post-treatment, flow cytometry for cell cycle, xenograft model Scientific reports Medium 41298459
2019 hnRNPR stabilizes CCNB1 mRNA; knockdown of CCNB1 abolishes hnRNPR-induced cell growth in gastric cancer, placing hnRNPR-CCNB1 as a functional axis regulating proliferation. hnRNPR knockdown with mRNA-level CCNB1 readout (mRNA stability implied), CCNB1 knockdown rescue of hnRNPR overexpression phenotype Aging Low 31527303
2022 RAD6A (E2 ubiquitin-conjugating enzyme) positively regulates CCNB1 expression through upregulation of H2B ubiquitination, promoting ESCC progression; RAD6A knockdown reduces both H2B ubiquitination and CCNB1 levels. RAD6A shRNA knockdown with H2B ubiquitination and CCNB1 protein-level readout, correlation analysis in tissues, xenograft Biological procedures online Low 35321657
2026 N-glycosylation of ERLIN2 at asparagine 106 enhances its interaction with CCNB1, leading to dysregulated (elevated) CCNB1 expression and accelerating HCC progression; MARCHF6 E3 ligase mediates ubiquitination degradation of ERLIN2, an effect more pronounced when N106 glycosylation is inhibited. Co-immunoprecipitation (ERLIN2-CCNB1 interaction), N106Q mutant analysis, MARCHF6 knockdown, N-glycosylation inhibition with CCNB1 protein readout Molecular and cellular biochemistry Low 42166002

Source papers

Stage 0 corpus · 99 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2021 MYC-targeted WDR4 promotes proliferation, metastasis, and sorafenib resistance by inducing CCNB1 translation in hepatocellular carcinoma. Cell death & disease 191 34244479
2018 Effect of CCNB1 silencing on cell cycle, senescence, and apoptosis through the p53 signaling pathway in pancreatic cancer. Journal of cellular physiology 156 30069972
2014 Chk1-induced CCNB1 overexpression promotes cell proliferation and tumor growth in human colorectal cancer. Cancer biology & therapy 131 24971465
2019 The circular RNA circ-Ccnb1 dissociates Ccnb1/Cdk1 complex suppressing cell invasion and tumorigenesis. Cancer letters 127 31199987
2018 Enhanced breast cancer progression by mutant p53 is inhibited by the circular RNA circ-Ccnb1. Cell death and differentiation 116 29795334
2019 HnRNPR-CCNB1/CENPF axis contributes to gastric cancer proliferation and metastasis. Aging 93 31527303
2018 FOXM1 promotes proliferation in human hepatocellular carcinoma cells by transcriptional activation of CCNB1. Biochemical and biophysical research communications 85 29705704
2019 MicroRNA-144 inhibits cell proliferation, migration and invasion in human hepatocellular carcinoma by targeting CCNB1. Cancer cell international 76 30651720
2015 Ubiquitin-specific protease 22 is a deubiquitinase of CCNB1. Cell discovery 76 27030811
2019 MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling. The Journal of cell biology 72 30674583
2016 ISL1, a novel regulator of CCNB1, CCNB2 and c-MYC genes, promotes gastric cancer cell proliferation and tumor growth. Oncotarget 63 27183908
2019 CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization. The Journal of cell biology 51 30674582
2017 Maternal mRNAs with distinct 3' UTRs define the temporal pattern of Ccnb1 synthesis during mouse oocyte meiotic maturation. Genes & development 51 28808066
2020 LncRNA CRNDE acts as an oncogene in cervical cancer through sponging miR-183 to regulate CCNB1 expression. Carcinogenesis 49 31605132
2014 Expression signature defined by FOXM1-CCNB1 activation predicts disease recurrence in non-muscle-invasive bladder cancer. Clinical cancer research : an official journal of the American Association for Cancer Research 47 24714775
2020 LINC00346 Acts as a Competing Endogenous RNA Regulating Development of Hepatocellular Carcinoma via Modulating CDK1/CCNB1 Axis. Frontiers in bioengineering and biotechnology 46 32133348
2008 Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression. Clinical cancer research : an official journal of the American Association for Cancer Research 45 18593994
2019 Degradation of CCNB1 mediated by APC11 through UBA52 ubiquitination promotes cell cycle progression and proliferation of non-small cell lung cancer cells. American journal of translational research 39 31814919
2021 CCNB1 promotes the development of hepatocellular carcinoma by mediating DNA replication in the cell cycle. Experimental biology and medicine (Maywood, N.J.) 38 34743578
2017 Requirement for CCNB1 in mouse spermatogenesis. Cell death & disease 38 29072697
2020 Positive Feedback Stimulation of Ccnb1 and Mos mRNA Translation by MAPK Cascade During Mouse Oocyte Maturation. Frontiers in cell and developmental biology 36 33282880
2019 Regulating the CCNB1 gene can affect cell proliferation and apoptosis in pituitary adenomas and activate epithelial-to-mesenchymal transition. Oncology letters 34 31611974
2022 MiR-139-5p Targeting CCNB1 Modulates Proliferation, Migration, Invasion and Cell Cycle in Lung Adenocarcinoma. Molecular biotechnology 31 35181869
2008 The Tg(ccnb1:EGFP) transgenic zebrafish line labels proliferating cells during retinal development and regeneration. Molecular vision 30 18509551
2013 Association study of germline variants in CCNB1 and CDK1 with breast cancer susceptibility, progression, and survival among Chinese Han women. PloS one 29 24386390
2022 CCNB1 and AURKA are critical genes for prostate cancer progression and castration-resistant prostate cancer resistant to vinblastine. Frontiers in endocrinology 28 36601001
2021 MEOX1 suppresses the progression of lung cancer cells by inhibiting the cell-cycle checkpoint gene CCNB1. Environmental toxicology 28 34837450
2023 A pancancer analysis of the oncogenic role of cyclin B1 (CCNB1) in human tumors. Scientific reports 27 37758792
2021 An Oxidative Stress-Related Gene Pair (CCNB1/PKD1), Competitive Endogenous RNAs, and Immune-Infiltration Patterns Potentially Regulate Intervertebral Disc Degeneration Development. Frontiers in immunology 24 34858418
2020 VPS33B modulates c-Myc/p53/miR-192-3p to target CCNB1 suppressing the growth of non-small cell lung cancer. Molecular therapy. Nucleic acids 24 33425490
2021 Huanglian decoction suppresses the growth of hepatocellular carcinoma cells by reducing CCNB1 expression. World journal of gastroenterology 23 33776365
2020 Unravelling the Role of miR-20b-5p, CCNB1, HMGA2 and E2F7 in Development and Progression of Non-Small Cell Lung Cancer (NSCLC). Biology 23 32752229
2021 CircNFIX promotes progression of pituitary adenoma via CCNB1 by sponging miR-34a -5p. Molecular and cellular endocrinology 22 33359304
2021 Dihydroartemisinin Suppresses the Tumorigenesis and Cycle Progression of Colorectal Cancer by Targeting CDK1/CCNB1/PLK1 Signaling. Frontiers in oncology 22 34796115
2019 CCNB1 affects cavernous sinus invasion in pituitary adenomas through the epithelial-mesenchymal transition. Journal of translational medicine 22 31585531
2012 Transcription factor STOX1A promotes mitotic entry by binding to the CCNB1 promotor. PloS one 21 22253775
2021 Hsa-mir-3163 and CCNB1 may be potential biomarkers and therapeutic targets for androgen receptor positive triple-negative breast cancer. PloS one 20 34797837
2022 The clinical significance of cyclin B1 (CCNB1) in invasive breast cancer with emphasis on its contribution to lymphovascular invasion development. Breast cancer research and treatment 19 36418517
2020 CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1. OncoTargets and therapy 19 33299327
2015 BUB1B promotes multiple myeloma cell proliferation through CDC20/CCNB axis. Medical oncology (Northwood, London, England) 19 25698537
2023 Z-Guggulsterone Induces Cell Cycle Arrest and Apoptosis by Targeting the p53/CCNB1/PLK1 Pathway in Triple-Negative Breast Cancer. ACS omega 18 36687039
2025 METTL3-modified exosomes from adipose-derived stem cells enhance the proliferation and migration of dermal fibroblasts by mediating m6A modification of CCNB1 mRNA. Archives of dermatological research 17 39954139
2020 has_circ_CCNB1 and has_circ_0009024 function as potential biomarkers for the diagnosis of type 2 diabetes mellitus. Journal of clinical laboratory analysis 17 32633001
2019 MicroRNA‑718 serves a tumor‑suppressive role in non‑small cell lung cancer by directly targeting CCNB1. International journal of molecular medicine 17 31746372
2011 Integrative genomic analysis identifies CCNB1 and CDC2 as candidate genes associated with meningioma recurrence. Cancer genetics 16 22137483
2022 Circ-CCNB1 Modulates Trophoblast Proliferation and Invasion in Spontaneous Abortion by Regulating miR-223/SIAH1 axis. Endocrinology 15 35731831
2022 CCNB1 and CCNB2 involvement in the pathogenesis of psoriasis: a bioinformatics study. The Journal of international medical research 15 35949173
2021 Long noncoding RNA PTTG3P/miR-192-3p/CCNB1 axis is a potential biomarker of childhood asthma. International immunopharmacology 13 34717195
2017 Polymorphisms of CCNB1 Associated With the Clinical Outcomes of Platinum-Based Chemotherapy in Chinese NSCLC Patients. Journal of Cancer 13 29151966
2025 Integrated bioinformatics and molecular docking ıdentify CCNB1, CDK1, and CYP1A2 as therapeutic targets of phytochemicals in hepatocellular carcinoma. Naunyn-Schmiedeberg's archives of pharmacology 11 41099846
2020 RP11-295G20.2 facilitates hepatocellular carcinoma progression via the miR-6884-3p/CCNB1 pathway. Aging 11 32687483
1992 Human cyclin B1 gene (CCNB1) assigned to chromosome 5 (q13-qter). Somatic cell and molecular genetics 11 1386686
2024 Down-regulation of SLC14A1 in prostate cancer activates CDK1/CCNB1 and mTOR pathways and promotes tumor progression. Scientific reports 10 38942821
2023 Squalene epoxidase promotes breast cancer progression by regulating CCNB1 protein stability. Experimental cell research 10 37839786
2014 Genetic variants in CCNB1 associated with differential gene transcription and risk of coronary in-stent restenosis. Circulation. Cardiovascular genetics 10 24395923
2023 Resveratrol affects ccRCC cell senescence and macrophage polarization by regulating the stability of CCNB1 by RBM15. Epigenomics 9 37909116
2022 CCNB1, Negatively Regulated by miR-559, Promotes the Proliferation, Migration, and Invasion of Ovarian Carcinoma Cells. Molecular biotechnology 8 35262876
2022 CircPVT1 promotes the tumorigenesis and metastasis of osteosarcoma via mediation of miR-26b-5p/CCNB1 axis. Journal of bone and mineral metabolism 8 35648221
2019 Ultrasound-Guided Breast Biopsy: Improved Accuracy of 10-G Cable-Free Elite Compared With 14-G CCNB. The Journal of surgical research 8 31761441
2021 SIN3A Regulates Porcine Early Embryonic Development by Modulating CCNB1 Expression. Frontiers in cell and developmental biology 7 33692994
2016 Mouse-specific up-regulation of Ccnb1 expression by miR-199a-5p in keratinocyte. FEBS open bio 7 27833853
2022 Septin 9 controls CCNB1 stabilization via APC/CCDC20 during meiotic metaphase I/anaphase I transition in mouse oocytes. Cell proliferation 6 36354207
2021 Septin 4 controls CCNB1 stabilization via APC/CCDC20 during meiotic G2/M transition in mouse oocytes. Journal of cellular physiology 6 34189751
2024 AURKA promotes renal cell carcinoma progression via regulation of CCNB1 transcription. Heliyon 5 38655290
2017 Single-cell, single-mRNA analysis of Ccnb1 promoter regulation. Scientific reports 5 28522800
2025 SNRPB/CCNB1 axis promotes hepatocellular carcinoma progression and cisplatin resistance through enhancing lipid metabolism reprogramming. Journal of experimental & clinical cancer research : CR 4 40682115
2024 UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1. Journal of animal science and biotechnology 4 38858680
2023 Hsa_circ_0137652 Regulates miR-1205/CCNB1 Axis to Accelerate the Malignancy of Breast Cancer. Molecular biotechnology 4 36807271
2025 Identification of CCNB1 as a biomarker for cellular senescence in hepatocellular carcinoma: a bioinformatics and experimental validation study. Discover oncology 3 40128499
2024 Alternative cleavage and polyadenylation of the Ccnb1 mRNA defines accumulation of cyclin protein during the meiotic cell cycle. Nucleic acids research 3 38048302
2024 Kielin/chordin-like protein deficiency aggravates pressure overload-induced cardiac dysfunction and remodeling via P53/P21/CCNB1 signaling in mice. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 3 38421300
2023 The role of CXCL8 and CCNB1 in predicting hepatocellular carcinoma in the context of cirrhosis: implications for early detection and immune-based therapies. Journal of cancer research and clinical oncology 3 37391641
2022 RAD6 Positively Affects Tumorigenesis of Esophageal Squamous Cell Carcinoma by Regulating Histone Ubiquitination of CCNB1. Biological procedures online 3 35321657
2022 MM-CCNB: Essential protein prediction using MAX-MIN strategies and compartment of common neighboring approach. Computer methods and programs in biomedicine 3 36427433
2022 Differential expression of cyclins CCNB1 and CCNG1 is involved in the chondrocyte damage of kashin-beck disease. Frontiers in genetics 3 36588792
2000 Localization of new, microdissection- generated, anonymous markers and of the genes Pcsk1, Dhfr, Ndub13, and Ccnb1 to rat chromosome region 2q1. Cytogenetics and cell genetics 3 10773684
2024 CCNB1 may as a biomarker for the adipogenic differentiation of adipose-derived stem cells in the postoperative fat transplantation of breast cancer. Gland surgery 2 38323233
2021 [Retracted] MicroRNA‑718 serves a tumor‑suppressive role in non‑small cell lung cancer by directly targeting CCNB1. International journal of molecular medicine 2 34296291
2011 Silencing of Her2, CCNB1 and PKC Genes by siRNA Results in Prolonged Retardation of Neuroblastoma Cell Division. Acta naturae 2 22649691
2026 CircDCAF6 Modulates Proliferation and Apoptosis in Bovine Myoblasts Through the miR-181d/CCNB1 Axis. Probiotics and antimicrobial proteins 1 41518519
2025 Knockdown of CCNB1 alleviates high glucose-triggered trophoblast dysfunction during gestational diabetes via Wnt/β-catenin signaling pathway. Open medicine (Warsaw, Poland) 1 39822992
2025 HROB Induces Lung Adenocarcinoma Progression via ZC3HC1-CCNB1 Axis Regulation and Cell Cycle Dysregulation. Cancer science 1 40654113
2025 Ropivacaine-loaded tumor-derived vesicles targeting CCNB1 and autophagy in nasopharyngeal carcinoma. Materials today. Bio 1 41122232
2023 DEPDC1B enhances malignant phenotypes of multiple myeloma through upregulating CCNB1 and inhibiting p53 signaling pathway. Tissue & cell 1 37979396
2021 Deciphering the modes of human separase inhibition by securin and CDK1-CCNB1. Molecular & cellular oncology 1 34616878
2010 [The CCNB1, HER2, and PKC silencing induced by small interfering RNA decreases a division of different human cancer cells with interfering RNA decreases a different efficiency]. Molekuliarnaia biologiia 1 20198864
2026 AR-mediated YBX3 suppresses oocyte meiotic resumption in polycystic ovary syndrome by attenuating Pde3A and Ccnb1 expression. Journal of ovarian research 0 41673703
2026 Targeting CCNB1 Repressed Cartilage Degradation Induced by Inflammation Through NF-κB Pathway in Osteoarthritis. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 0 41731977
2026 Multi-omics analysis identifies CCNB1 as a cell cycle factor driving glioblastoma progression and its inhibition by resveratrol. PloS one 0 41838760
2026 Whole transcriptome sequencing identifies key characteristics and potential therapeutic efficacy biomarkers CD1B, CD247, and CCNB1 in cured tuberculosis patients. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 0 41871736
2026 MAPK1 phosphorylates CCNB1 at Ser128 to drive mitotic progression and cell proliferation in triple-negative breast cancer. Life sciences 0 41905453
2026 N-glycosylation of ERLIN2 promotes hepatocellular carcinoma progression by enhancing CCNB1 stability. Molecular and cellular biochemistry 0 42166002
2025 MND1 Promotes the Proliferation of Prostate Cancer Cell Via the CCNB1/p53 Signaling Pathway. Current cancer drug targets 0 40353402
2025 Retraction: LINC00346 Acts as a competing endogenous RNA regulating development of hepatocellular carcinoma via modulating CDK1/CCNB1 Axis. Frontiers in bioengineering and biotechnology 0 41280647
2025 The mechanism of luteolin suppressing pancreatic cancer (PC) via cyclin B1 (CCNB1)-mediated signalling. Scientific reports 0 41298459
2024 The role of GADD45G methylation in endometrial cancer: Insights into CDK1/CCNB1 activation and therapeutic opportunities. Journal of cancer research and therapeutics 0 39206984
2023 Hsa-circ-0006091 modulates the proliferation of hepatocellular carcinoma via the miR-622/CCNB1 axis. Turkish journal of medical sciences 0 38813026
2022 CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1 [Retraction]. OncoTargets and therapy 0 36275181
2022 miR-144 negatively regulates the effect of CCNB1 protein on the biological behavior of hepatoma cells. Cellular and molecular biology (Noisy-le-Grand, France) 0 36495514

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