Affinage

CCNB1

G2/mitotic-specific cyclin-B1 · UniProt P14635

Length
433 aa
Mass
48.3 kDa
Annotated
2026-04-28
98 papers in source corpus 31 papers cited in narrative 30 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CCNB1 (Cyclin B1) is the regulatory subunit of the CDK1–CCNB1 (MPF) kinase complex that drives the G2-to-M phase transition, with essential roles in mitotic entry, spindle assembly checkpoint (SAC) signaling, and separase inhibition. CDK1–CCNB1 localizes to unattached kinetochores via the MAD1 N-terminus and phosphorylates MPS1 at S281 to sustain SAC arrest, while also forming a CDK1–CCNB1–CKS1 inhibitory complex that directly blocks separase-mediated cohesin cleavage (PMID:30674583, PMID:30674582, PMID:34616878). CCNB1 abundance is tightly controlled: transcription is activated by FOXM1 (recruiting CBP/P300), NF-Y, E2F1 (recruited by AURKA), ISL1, and STOX1A (PMID:29705704, PMID:28522800, PMID:38655290, PMID:27183908, PMID:22253775); mRNA stability is modulated by m7G (WDR4/EIF2A) and m6A (METTL3) modifications (PMID:34244479, PMID:39954139); translational timing in oocytes depends on alternative polyadenylation-generated 3′-UTR variants regulated by a CPEB1/MAPK/CDK1 positive-feedback loop (PMID:28808066, PMID:33282880); and protein turnover is governed by APC/C–CDC20-mediated ubiquitination counteracted by the deubiquitinases USP22 and UCHL1 (PMID:27030811, PMID:38858680). MAPK1 directly phosphorylates CCNB1 at Ser128 to promote its nuclear translocation and mitotic entry, and conditional CCNB1 knockout in mouse male germ cells causes sterility through gonocyte/spermatogonia depletion, demonstrating its essential role in germ-cell proliferation (PMID:41905453, PMID:29072697).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1992 Medium

    Mapping CCNB1 to human chromosome 5q13-qter established its genomic location, a prerequisite for genetic and functional studies.

    Evidence Southern blot of human × Chinese hamster somatic cell hybrid panels

    PMID:1386686

    Open questions at the time
    • Precise chromosomal band assignment not yet defined
    • No functional data at this stage
  2. 2012 Medium

    Identification of STOX1A as a direct transcriptional activator of CCNB1 via promoter binding began to define the transcription factor network controlling CCNB1 expression and mitotic entry.

    Evidence ChIP assay and overexpression/knockdown in human cells

    PMID:22253775

    Open questions at the time
    • No combinatorial analysis with other CCNB1 transcription factors
    • Single lab without independent replication
  3. 2015 High

    Discovery that USP22 deubiquitinates CCNB1 to antagonize APC/C-mediated degradation—and that CDK1 phosphorylates USP22 to enhance this activity—revealed a positive-feedback stabilization loop governing mitotic CCNB1 levels.

    Evidence In vitro deubiquitination assay, Co-IP, cell cycle fractionation, siRNA knockdown

    PMID:27030811

    Open questions at the time
    • Structural basis of USP22–CCNB1 interaction unknown
    • Relative contribution of USP22 vs. other DUBs in vivo uncharacterized
  4. 2016 Medium

    ISL1 was shown to directly bind the CCNB1 promoter and activate transcription, expanding the set of tissue-specific transcription factors (alongside STOX1A) that drive CCNB1 in proliferating cells.

    Evidence ChIP assay, luciferase reporter, siRNA knockdown in gastric cancer cells

    PMID:27183908

    Open questions at the time
    • Relative hierarchy among CCNB1 transcription factors not established
    • Single cancer type tested
  5. 2017 High

    Alternative polyadenylation was shown to generate CCNB1 mRNA isoforms with distinct 3′ UTRs that are translated at different meiotic stages, establishing a post-transcriptional timing mechanism for Cyclin B1 accumulation in oocytes.

    Evidence Gene editing of proximal/distal polyadenylation elements in mouse oocytes with phenotypic analysis

    PMID:28808066

    Open questions at the time
    • Trans-acting factors for each APA variant incompletely defined
    • Whether similar APA control operates in somatic cells unknown
  6. 2017 High

    Conditional CCNB1 knockout in mouse male germ cells demonstrated that CCNB1 is essential for gonocyte and spermatogonia proliferation but dispensable for spermatocyte meiosis, delineating its cell-type-specific requirement.

    Evidence Conditional knockout mice with histology, apoptosis assays, and gene expression analysis

    PMID:29072697

    Open questions at the time
    • Whether CCNB2 compensates during meiosis not formally tested
    • Molecular targets of CDK1–CCNB1 in spermatogonia not identified
  7. 2017 Medium

    NF-Y was established as a key activator of the CCNB1 promoter that controls the frequency of promoter activation (ON/OFF cycling) rather than Pol II elongation rate, providing a mechanistic basis for transcriptional burst regulation.

    Evidence Single-molecule mRNA imaging with dominant-negative NF-YA and histone ChIP

    PMID:28522800

    Open questions at the time
    • How NF-Y cooperates with FOXM1 and other factors at the endogenous locus not resolved
    • Single-copy transgene may not fully recapitulate endogenous chromatin context
  8. 2018 Medium

    FOXM1 was shown to directly bind the CCNB1 promoter and activate its transcription, with loss of CCNB1 abolishing FOXM1-driven proliferation, establishing FOXM1 as a critical upstream transcriptional regulator.

    Evidence ChIP, luciferase reporter, siRNA knockdown and rescue in HCC cells

    PMID:29705704

    Open questions at the time
    • Whether FOXM1 is the dominant transcription factor for CCNB1 across tissues unknown
    • Epigenetic co-regulators beyond CBP/P300 not characterized
  9. 2019 High

    Two companion studies established CDK1–CCNB1 as an integral spindle assembly checkpoint component: it localizes to unattached kinetochores via MAD1 and phosphorylates MPS1 at S281 to sustain checkpoint signaling, with PP2A-B55 acting as the opposing phosphatase that becomes active only when CCNB1 falls below a threshold.

    Evidence Reciprocal Co-IP, proteomics, phospho-mutant analysis, kinetochore tethering bypass, epistasis with MPS1 inhibition in human cells

    PMID:30674582 PMID:30674583

    Open questions at the time
    • Precise stoichiometry of CDK1–CCNB1 at kinetochores not determined
    • Whether CDK1–CCNB1 phosphorylates additional SAC substrates at kinetochores not fully explored
  10. 2019 Medium

    APC11 was identified as the E3 ligase component mediating UBA52-dependent CCNB1 ubiquitination for proteasomal degradation, defining a specific ubiquitin-chain initiation mechanism for CCNB1 turnover.

    Evidence Co-IP/proteomics, siRNA epistasis, and xenograft assays

    PMID:31814919

    Open questions at the time
    • Ubiquitin chain type specificity beyond K27 linkage not clarified in this study
    • Relationship to canonical APC/C-CDC20 D-box recognition pathway needs integration
  11. 2020 Medium

    ERK1/2 and CDK1 were shown to cooperate in a positive-feedback loop that activates CCNB1 mRNA translation in oocytes by phosphorylating and degrading the translational repressor CPEB1, connecting signaling cascades to the APA-based translational timing mechanism.

    Evidence Pharmacological inhibition of MAPK/CDK1 with CPEB1 phosphorylation analysis in mouse oocytes

    PMID:33282880

    Open questions at the time
    • Direct phosphorylation sites on CPEB1 by each kinase not fully mapped
    • Whether this loop operates in somatic G2/M not tested
  12. 2021 High

    Cryo-EM structures of the human separase–CDK1–CCNB1–CKS1 complex revealed the molecular basis of separase inhibition by CDK1–CCNB1, distinct from securin-mediated inhibition, explaining how two independent inhibitory mechanisms safeguard sister chromatid cohesion.

    Evidence Cryo-EM/crystallographic structure determination with functional validation

    PMID:34616878

    Open questions at the time
    • Dynamic interconversion between securin-bound and CDK1-CCNB1-bound separase states in vivo not characterized
    • How the two inhibitory modes are coordinated during mitotic exit remains unclear
  13. 2021 Medium

    WDR4-mediated m7G methylation of CCNB1 mRNA was shown to enhance its stability and translation via EIF2A recruitment, revealing an epitranscriptomic layer of CCNB1 regulation.

    Evidence m7G profiling, RIP assay, mRNA stability assays in HCC cells

    PMID:34244479

    Open questions at the time
    • Whether m7G and m6A modifications on CCNB1 mRNA interact or are mutually exclusive unknown
    • In vivo validation lacking
  14. 2024 Medium

    UCHL1 was identified as a second deubiquitinase (in addition to USP22) that directly stabilizes CCNB1 protein by inhibiting its proteasomal degradation, indicating redundant DUB-mediated protection of Cyclin B1.

    Evidence Co-IP, half-life assay, siRNA knockdown/overexpression in porcine granulosa cells

    PMID:38858680

    Open questions at the time
    • Whether UCHL1 and USP22 act on the same or different ubiquitin chain types on CCNB1 unknown
    • Relevance in human somatic cells not confirmed
  15. 2025 Medium

    METTL3-mediated m6A modification was shown to stabilize CCNB1 mRNA, adding a second epitranscriptomic control layer alongside m7G modification and linking CCNB1 regulation to intercellular communication via exosomes.

    Evidence MeRIP, mRNA stability assay, functional rescue in dermal fibroblasts

    PMID:39954139

    Open questions at the time
    • m6A reader identity for CCNB1 mRNA not identified
    • Relative contribution of m6A vs. m7G to CCNB1 mRNA stability not compared
  16. 2026 High

    MAPK1 was shown to directly phosphorylate CCNB1 at Ser128 to promote its nuclear translocation and G2-to-prophase transition, establishing a direct kinase–substrate link between mitogenic signaling and mitotic entry.

    Evidence In vitro kinase assay, TurboID proximity labeling, phospho-mutant rescue, nuclear-cytoplasmic fractionation in TNBC cells and xenografts

    PMID:41905453

    Open questions at the time
    • Whether Ser128 phosphorylation affects CDK1 binding or catalytic activity unknown
    • Interplay with CRM1/importin-mediated nuclear transport not defined

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the multiple transcriptional, epitranscriptomic, translational, and post-translational inputs converge quantitatively to set the threshold of CDK1–CCNB1 activity required for mitotic commitment remains an open systems-level question.
  • No integrated quantitative model of CCNB1 accumulation combining transcription, RNA modification, translation, and degradation
  • In vivo contribution of individual DUBs and E3 ligases to CCNB1 steady-state levels not compared
  • Structural basis of MAPK1-mediated Ser128 phosphorylation and its effect on CDK1–CCNB1 complex dynamics unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 4 GO:0140096 catalytic activity, acting on a protein 2
Localization
GO:0005634 nucleus 2 GO:0005694 chromosome 2 GO:0005829 cytosol 2
Pathway
R-HSA-1640170 Cell Cycle 8 R-HSA-74160 Gene expression (Transcription) 5 R-HSA-392499 Metabolism of proteins 4 R-HSA-162582 Signal Transduction 2
Partners
CDK1FOXM1GADD45GMAD1MAPK1SQLEUCHL1USP22
Complex memberships
APC/C-CDC20 (substrate)CDK1-CCNB1 (MPF)CDK1-CCNB1-CKS1-separase inhibitory complex

Evidence

Reading pass · 30 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2019 CDK1-CCNB1 localizes to unattached kinetochores in an MPS1-dependent manner through interaction with the first 100 amino acids of MAD1, forming a positive feedback loop that promotes timely MPS1 recruitment to kinetochores and sustained spindle checkpoint arrest, establishing CDK1-CCNB1 as an integral component of the spindle assembly checkpoint. Complementary proteomic analysis, Co-IP, cell biological analysis (live imaging, immunofluorescence), and epistasis with MPS1 inhibition The Journal of cell biology High 30674583
2019 CDK1-CCNB1 phosphorylates MPS1 at S281 in its kinetochore-binding domain to promote MPS1 engagement with unattached kinetochores, enabling spindle checkpoint signaling; PP2A-B55 (itself negatively regulated by CDK1-CCNB1) counteracts this phosphorylation, and only becomes fully active once CCNB1 concentration falls below a threshold, prolonging the checkpoint-responsive period. Phospho-mutant analysis (MPS1-S281A), direct kinetochore tethering bypass experiment, cell cycle synchronization, immunofluorescence, and kinase/phosphatase epistasis The Journal of cell biology High 30674582
2021 Structures of human separase bound to CDK1-CCNB1-CKS1 complex reveal molecular mechanisms by which this complex inhibits separase's cohesin-cleavage protease activity, distinct from the securin inhibition mode. Structural analysis (cryo-EM/crystallography) of human separase-CDK1-CCNB1-CKS1 complex Molecular & cellular oncology High 34616878
2015 USP22 is a deubiquitinase of CCNB1 that stabilizes CCNB1 by antagonizing APC/C-mediated proteasomal degradation in a cell cycle-specific manner; CDK1 phosphorylates USP22 to enhance its deubiquitinating activity toward CCNB1, and APC/C-CDC20 targets USP22 for degradation during M phase exit, allowing subsequent CCNB1 degradation. Co-IP, in vitro deubiquitination assay, cell cycle-specific fractionation, siRNA knockdown, USP22 phosphorylation analysis, xenograft tumor assay Cell discovery High 27030811
2019 circ-Ccnb1 interacts with both Ccnb1 and Cdk1 proteins and dissociates the Ccnb1-Cdk1 complex by forming a larger complex (circ-Ccnb1/Ccnb1/Cdk1), thereby suppressing Ccnb1-driven cell migration, invasion, proliferation and survival; ectopic delivery of circ-Ccnb1 inhibited tumor growth in vivo. Co-IP/RNA pulldown, immunofluorescence (cytosolic/nuclear complex localization), in vivo tumor delivery experiments Cancer letters Medium 31199987
2018 circ-Ccnb1 precipitates wild-type p53 via H2AX as a bridge, allowing Bclaf1 to bind Bcl2 and promote cell survival; in p53 mutant cells, circ-Ccnb1 instead forms a complex with H2AX and Bclaf1, inducing cell death. Proteomics, RNA pulldown, Co-IP, microarray analysis, in vivo tumor delivery Cell death and differentiation Medium 29795334
2021 WDR4, an m7G methyltransferase, promotes CCNB1 mRNA stability and translation by enhancing the binding of translation initiation factor EIF2A to CCNB1 mRNA; this is part of an MYC/WDR4/CCNB1 signaling axis in HCC. m7G methylation profiling, RIP assay, mRNA stability assays, Western blot, functional rescue experiments Cell death & disease Medium 34244479
2018 FOXM1 directly binds to the promoter region of CCNB1 and transcriptionally activates CCNB1 expression to drive HCC cell proliferation; loss of CCNB1 abolishes FOXM1-driven proliferation. ChIP assay, luciferase reporter assay, siRNA knockdown, rescue experiments Biochemical and biophysical research communications Medium 29705704
2020 FOXM1 promotes CCNB1 transcription in cervical squamous cell carcinoma by binding to the CCNB1 promoter and recruiting the histone acetyltransferase CBP/P300, thereby increasing histone acetylation at the CCNB1 promoter locus. ChIP assay, histone ChIP-Seq analysis, luciferase reporter assay, siRNA knockdown, rescue experiments OncoTargets and therapy Low 33299327
2024 AURKA interacts with transcription factor E2F1 and recruits it to the CCNB1 promoter to drive CCNB1 transcription, thereby promoting renal cell carcinoma progression. Co-IP, ChIP assay, luciferase reporter assay, siRNA knockdown, xenograft model Heliyon Medium 38655290
2012 The transcription factor STOX1A directly binds to the CCNB1 promoter (demonstrated by chromatin immunoprecipitation) and upregulates cyclin B1 expression to promote mitotic entry. ChIP assay, siRNA knockdown/overexpression, gene expression profiling PloS one Medium 22253775
2016 ISL1 transcription factor activates CCNB1 expression in gastric cancer by binding to conserved sites on the CCNB1 promoter or enhancer, thereby facilitating G2/M cell cycle transition. ChIP assay, luciferase reporter assay, siRNA knockdown, xenograft model Oncotarget Medium 27183908
2019 APC11, via UBA52-mediated ubiquitination, promotes CCNB1 degradation; APC11 knockdown causes G2/M arrest even in the presence of overexpressed UBA52, establishing APC11 as the E3 ligase mediating UBA52-dependent CCNB1 ubiquitylation and subsequent proteasomal degradation. Co-IP/proteomics, siRNA knockdown, overexpression rescue, xenograft tumor assay American journal of translational research Medium 31814919
2017 Timing of Ccnb1 mRNA translation during mouse oocyte meiotic maturation is determined by transcripts with different 3' UTRs generated through alternative polyadenylation; the short 3' UTR mRNA is translated early (setting meiotic re-entry timing) while the long 3' UTR mRNA drives Cyclin B1 accumulation during MI/MII transition, as demonstrated by in vivo loss-of-function mutations in polyadenylation elements. Gene editing in mice (mutations in proximal and distal polyadenylation elements), in vivo loss-of-function experiments, mRNA variant analysis Genes & development High 28808066 38048302
2020 ERK1/2 (MAPK cascade) activation promotes Ccnb1 mRNA translation during mouse oocyte maturation by triggering CPEB1 phosphorylation and degradation; CDK1 activity also contributes to CPEB1 phosphorylation (but not degradation); both kinases cooperate in a positive feedback loop to activate Ccnb1 translation. Pharmacological inhibition of MAPK/CDK1, CPEB1 phosphorylation analysis, meiotic maturation assays in mouse oocytes Frontiers in cell and developmental biology Medium 33282880
2021 Septin 4 regulates CCNB1 protein stabilization via APC/C-CDC20 during meiotic G2/M transition in mouse oocytes; Septin 4 depletion reduces CCNB1 accumulation and causes GV arrest, while CDC20 levels paradoxically increase; overexpressing CCNB1 or depleting CDC20 rescues GVBD. siRNA microinjection, mRNA microinjection rescue, immunofluorescence, Co-IP, time-lapse imaging, Western blot Journal of cellular physiology Medium 34189751
2022 Septin 9 depletion in mouse oocytes prevents APC/C-CDC20-mediated CCNB1 degradation at the MI/AI transition by disrupting kinetochore-microtubule attachment and maintaining SAC (Mad1/BubR1) activity; CDC20 mRNA microinjection partially rescues the arrest. siRNA and mRNA microinjection, time-lapse live imaging, immunofluorescence, Western blot, SAC marker analysis Cell proliferation Medium 36354207
2017 CCNB1 is required for normal proliferation of gonocytes and spermatogonia in mouse spermatogenesis; conditional knockout of CCNB1 in male germ cells causes sterility due to germ cell depletion and increased apoptosis, while ablation in premeiotic spermatocytes does not affect meiosis, indicating CCNB1 is dispensable for meiosis of spermatocytes. Conditional knockout mice, histological analysis, apoptosis assays, let-7 miRNA and Lin28a expression analysis Cell death & disease High 29072697
2022 Circ-CCNB1 inhibits trophoblast proliferation and invasion by sponging miR-223, which in turn targets SIAH1; elevated SIAH1 promotes CCNB1 nuclear translocation, and circ-CCNB1/miR-223/SIAH1 axis modulates nuclear CCNB1 levels to regulate trophoblast function in spontaneous abortion. siRNA knockdown, mRNA overexpression, RNA pulldown, dual-luciferase reporter assay, immunofluorescence for nuclear localization Endocrinology Medium 35731831
2025 METTL3 upregulates CCNB1 expression through m6A modification of CCNB1 mRNA, enhancing its stability; METTL3-modified ASC-exosomes transfer this effect to dermal fibroblasts to enhance their proliferation and migration. Methylated RNA immunoprecipitation (MeRIP), actinomycin D mRNA stability assay, siRNA knockdown, overexpression, Western blot Archives of dermatological research Medium 39954139
2023 SQLE (squalene epoxidase) physically interacts with CCNB1 (demonstrated by Co-IP and immunofluorescence) and reduces CCNB1 ubiquitination, thereby stabilizing the CCNB1 protein and decreasing intracellular ROS to inhibit ferroptosis in breast cancer. Co-IP, immunofluorescence, ubiquitination assay, ROS measurement, xenograft assay Experimental cell research Medium 37839786
2024 UCHL1 directly binds CCNB1, prolongs CCNB1 half-life, and inhibits its degradation (deubiquitinase activity), thereby promoting porcine granulosa cell proliferation; the flavonoid isovitexin enhances UCHL1 enzyme activity to further promote proliferation. Co-IP, half-life assay, siRNA knockdown and overexpression, cell proliferation assays Journal of animal science and biotechnology Medium 38858680
2021 SIN3A depletion in porcine early embryos drastically reduces CCNB1 transcript levels; injection of exogenous CCNB1 mRNA into SIN3A-depleted embryos partially rescues development past the two-cell block, placing SIN3A upstream of CCNB1 in embryonic genome activation. siRNA knockdown, RNA-seq, mRNA microinjection rescue, immunofluorescence Frontiers in cell and developmental biology Medium 33692994
2025 SNRPB regulates CCNB1 expression through FOXM1-mediated transcriptional activation; SNRPB interacts with FOXM1 (demonstrated by Co-IP), and SNRPB/FOXM1 promote CCNB1 transcription (demonstrated by ChIP); CCNB1 knockdown reverses proliferative and tumorigenic effects of SNRPB overexpression. ChIP, Co-IP, siRNA knockdown, functional assays, in vivo experiments Journal of experimental & clinical cancer research Medium 40682115
2024 GADD45G physically interacts with CDK1 and CCNB1 (demonstrated by Co-IP), and negatively regulates their expression; reduced GADD45G expression (due to promoter methylation) leads to CDK1/CCNB1 upregulation and enhanced invasive behavior in endometrial cancer. Co-immunoprecipitation, methylation-specific PCR, siRNA knockdown, in vitro and in vivo functional assays Journal of cancer research and therapeutics Medium 39206984
2025 HROB interacts with ZC3HC1 and reduces ZC3HC1 phosphorylation at Ser354, which facilitates K27-linked ubiquitination of CCNB1 and promotes its proteasomal degradation, impairing G2-to-M transition and suppressing cell proliferation in lung adenocarcinoma. Co-IP, ubiquitination assay, phospho-mutant analysis, cell cycle analysis, xenograft model Cancer science Medium 40654113
2026 MAPK1 (ERK2) directly phosphorylates CCNB1 at serine 128 (demonstrated by in vitro kinase assay and co-IP), promoting CCNB1 nuclear translocation and the G2-to-prophase transition; a phospho-mimetic CCNB1-S128D mutant partially rescues proliferation defects caused by MAPK1 knockdown in TNBC. TurboID proximity labeling + mass spectrometry, Co-IP, in vitro kinase assay, phospho-mutant analysis, nuclear-cytoplasmic fractionation, immunofluorescence, cell cycle analysis, xenograft model Life sciences High 41905453
2014 Chk1 positively regulates CCNB1 protein expression in colorectal cancer cells; Chk1 repression leads to decreased CCNB1 protein and mRNA levels, and CCNB1 knockdown causes G2/M arrest with associated changes in CDC25c and CDK1 expression. siRNA knockdown, Western blot, flow cytometry, xenograft tumor growth assay Cancer biology & therapy Medium 24971465
2017 NF-Y (NF-YA) is a key transcriptional activator of the Ccnb1 promoter; a dominant-negative NF-YA mutant increases the promoter 'OFF'/'ON' time ratio without altering Pol II firing rates during the 'ON' period, suggesting NF-Y controls promoter activation frequency rather than elongation rate at the Ccnb1 gene. Single-copy promoter transgene system, live single-molecule mRNA imaging, ChIP (H3K4me2, H3K79me2), dominant-negative NF-YA expression Scientific reports Medium 28522800
1992 Human CCNB1 was mapped to chromosome 5 (region q13-qter) by Southern blot analysis of human × Chinese hamster somatic cell hybrid panels. Somatic cell hybrid panel analysis, Southern blot Somatic cell and molecular genetics Medium 1386686

Source papers

Stage 0 corpus · 98 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2021 MYC-targeted WDR4 promotes proliferation, metastasis, and sorafenib resistance by inducing CCNB1 translation in hepatocellular carcinoma. Cell death & disease 190 34244479
2018 Effect of CCNB1 silencing on cell cycle, senescence, and apoptosis through the p53 signaling pathway in pancreatic cancer. Journal of cellular physiology 156 30069972
2014 Chk1-induced CCNB1 overexpression promotes cell proliferation and tumor growth in human colorectal cancer. Cancer biology & therapy 130 24971465
2019 The circular RNA circ-Ccnb1 dissociates Ccnb1/Cdk1 complex suppressing cell invasion and tumorigenesis. Cancer letters 124 31199987
2018 Enhanced breast cancer progression by mutant p53 is inhibited by the circular RNA circ-Ccnb1. Cell death and differentiation 114 29795334
2019 HnRNPR-CCNB1/CENPF axis contributes to gastric cancer proliferation and metastasis. Aging 93 31527303
2018 FOXM1 promotes proliferation in human hepatocellular carcinoma cells by transcriptional activation of CCNB1. Biochemical and biophysical research communications 85 29705704
2019 MicroRNA-144 inhibits cell proliferation, migration and invasion in human hepatocellular carcinoma by targeting CCNB1. Cancer cell international 75 30651720
2015 Ubiquitin-specific protease 22 is a deubiquitinase of CCNB1. Cell discovery 73 27030811
2019 MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling. The Journal of cell biology 70 30674583
2016 ISL1, a novel regulator of CCNB1, CCNB2 and c-MYC genes, promotes gastric cancer cell proliferation and tumor growth. Oncotarget 63 27183908
2019 CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization. The Journal of cell biology 50 30674582
2017 Maternal mRNAs with distinct 3' UTRs define the temporal pattern of Ccnb1 synthesis during mouse oocyte meiotic maturation. Genes & development 50 28808066
2020 LncRNA CRNDE acts as an oncogene in cervical cancer through sponging miR-183 to regulate CCNB1 expression. Carcinogenesis 48 31605132
2014 Expression signature defined by FOXM1-CCNB1 activation predicts disease recurrence in non-muscle-invasive bladder cancer. Clinical cancer research : an official journal of the American Association for Cancer Research 47 24714775
2020 LINC00346 Acts as a Competing Endogenous RNA Regulating Development of Hepatocellular Carcinoma via Modulating CDK1/CCNB1 Axis. Frontiers in bioengineering and biotechnology 45 32133348
2008 Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression. Clinical cancer research : an official journal of the American Association for Cancer Research 45 18593994
2019 Degradation of CCNB1 mediated by APC11 through UBA52 ubiquitination promotes cell cycle progression and proliferation of non-small cell lung cancer cells. American journal of translational research 38 31814919
2017 Requirement for CCNB1 in mouse spermatogenesis. Cell death & disease 38 29072697
2021 CCNB1 promotes the development of hepatocellular carcinoma by mediating DNA replication in the cell cycle. Experimental biology and medicine (Maywood, N.J.) 37 34743578
2020 Positive Feedback Stimulation of Ccnb1 and Mos mRNA Translation by MAPK Cascade During Mouse Oocyte Maturation. Frontiers in cell and developmental biology 35 33282880
2019 Regulating the CCNB1 gene can affect cell proliferation and apoptosis in pituitary adenomas and activate epithelial-to-mesenchymal transition. Oncology letters 33 31611974
2022 MiR-139-5p Targeting CCNB1 Modulates Proliferation, Migration, Invasion and Cell Cycle in Lung Adenocarcinoma. Molecular biotechnology 31 35181869
2008 The Tg(ccnb1:EGFP) transgenic zebrafish line labels proliferating cells during retinal development and regeneration. Molecular vision 30 18509551
2013 Association study of germline variants in CCNB1 and CDK1 with breast cancer susceptibility, progression, and survival among Chinese Han women. PloS one 29 24386390
2022 CCNB1 and AURKA are critical genes for prostate cancer progression and castration-resistant prostate cancer resistant to vinblastine. Frontiers in endocrinology 27 36601001
2021 MEOX1 suppresses the progression of lung cancer cells by inhibiting the cell-cycle checkpoint gene CCNB1. Environmental toxicology 25 34837450
2023 A pancancer analysis of the oncogenic role of cyclin B1 (CCNB1) in human tumors. Scientific reports 24 37758792
2021 An Oxidative Stress-Related Gene Pair (CCNB1/PKD1), Competitive Endogenous RNAs, and Immune-Infiltration Patterns Potentially Regulate Intervertebral Disc Degeneration Development. Frontiers in immunology 24 34858418
2020 VPS33B modulates c-Myc/p53/miR-192-3p to target CCNB1 suppressing the growth of non-small cell lung cancer. Molecular therapy. Nucleic acids 24 33425490
2021 CircNFIX promotes progression of pituitary adenoma via CCNB1 by sponging miR-34a -5p. Molecular and cellular endocrinology 22 33359304
2021 Huanglian decoction suppresses the growth of hepatocellular carcinoma cells by reducing CCNB1 expression. World journal of gastroenterology 22 33776365
2021 Dihydroartemisinin Suppresses the Tumorigenesis and Cycle Progression of Colorectal Cancer by Targeting CDK1/CCNB1/PLK1 Signaling. Frontiers in oncology 22 34796115
2019 CCNB1 affects cavernous sinus invasion in pituitary adenomas through the epithelial-mesenchymal transition. Journal of translational medicine 22 31585531
2020 Unravelling the Role of miR-20b-5p, CCNB1, HMGA2 and E2F7 in Development and Progression of Non-Small Cell Lung Cancer (NSCLC). Biology 21 32752229
2012 Transcription factor STOX1A promotes mitotic entry by binding to the CCNB1 promotor. PloS one 21 22253775
2021 Hsa-mir-3163 and CCNB1 may be potential biomarkers and therapeutic targets for androgen receptor positive triple-negative breast cancer. PloS one 20 34797837
2020 CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1. OncoTargets and therapy 19 33299327
2015 BUB1B promotes multiple myeloma cell proliferation through CDC20/CCNB axis. Medical oncology (Northwood, London, England) 19 25698537
2023 Z-Guggulsterone Induces Cell Cycle Arrest and Apoptosis by Targeting the p53/CCNB1/PLK1 Pathway in Triple-Negative Breast Cancer. ACS omega 18 36687039
2022 The clinical significance of cyclin B1 (CCNB1) in invasive breast cancer with emphasis on its contribution to lymphovascular invasion development. Breast cancer research and treatment 18 36418517
2020 has_circ_CCNB1 and has_circ_0009024 function as potential biomarkers for the diagnosis of type 2 diabetes mellitus. Journal of clinical laboratory analysis 17 32633001
2019 MicroRNA‑718 serves a tumor‑suppressive role in non‑small cell lung cancer by directly targeting CCNB1. International journal of molecular medicine 17 31746372
2011 Integrative genomic analysis identifies CCNB1 and CDC2 as candidate genes associated with meningioma recurrence. Cancer genetics 16 22137483
2022 Circ-CCNB1 Modulates Trophoblast Proliferation and Invasion in Spontaneous Abortion by Regulating miR-223/SIAH1 axis. Endocrinology 15 35731831
2025 METTL3-modified exosomes from adipose-derived stem cells enhance the proliferation and migration of dermal fibroblasts by mediating m6A modification of CCNB1 mRNA. Archives of dermatological research 14 39954139
2022 CCNB1 and CCNB2 involvement in the pathogenesis of psoriasis: a bioinformatics study. The Journal of international medical research 14 35949173
2017 Polymorphisms of CCNB1 Associated With the Clinical Outcomes of Platinum-Based Chemotherapy in Chinese NSCLC Patients. Journal of Cancer 13 29151966
2021 Long noncoding RNA PTTG3P/miR-192-3p/CCNB1 axis is a potential biomarker of childhood asthma. International immunopharmacology 12 34717195
2020 RP11-295G20.2 facilitates hepatocellular carcinoma progression via the miR-6884-3p/CCNB1 pathway. Aging 11 32687483
1992 Human cyclin B1 gene (CCNB1) assigned to chromosome 5 (q13-qter). Somatic cell and molecular genetics 11 1386686
2014 Genetic variants in CCNB1 associated with differential gene transcription and risk of coronary in-stent restenosis. Circulation. Cardiovascular genetics 10 24395923
2025 Integrated bioinformatics and molecular docking ıdentify CCNB1, CDK1, and CYP1A2 as therapeutic targets of phytochemicals in hepatocellular carcinoma. Naunyn-Schmiedeberg's archives of pharmacology 9 41099846
2023 Squalene epoxidase promotes breast cancer progression by regulating CCNB1 protein stability. Experimental cell research 9 37839786
2023 Resveratrol affects ccRCC cell senescence and macrophage polarization by regulating the stability of CCNB1 by RBM15. Epigenomics 9 37909116
2024 Down-regulation of SLC14A1 in prostate cancer activates CDK1/CCNB1 and mTOR pathways and promotes tumor progression. Scientific reports 8 38942821
2022 CCNB1, Negatively Regulated by miR-559, Promotes the Proliferation, Migration, and Invasion of Ovarian Carcinoma Cells. Molecular biotechnology 8 35262876
2022 CircPVT1 promotes the tumorigenesis and metastasis of osteosarcoma via mediation of miR-26b-5p/CCNB1 axis. Journal of bone and mineral metabolism 8 35648221
2019 Ultrasound-Guided Breast Biopsy: Improved Accuracy of 10-G Cable-Free Elite Compared With 14-G CCNB. The Journal of surgical research 8 31761441
2021 SIN3A Regulates Porcine Early Embryonic Development by Modulating CCNB1 Expression. Frontiers in cell and developmental biology 7 33692994
2016 Mouse-specific up-regulation of Ccnb1 expression by miR-199a-5p in keratinocyte. FEBS open bio 7 27833853
2022 Septin 9 controls CCNB1 stabilization via APC/CCDC20 during meiotic metaphase I/anaphase I transition in mouse oocytes. Cell proliferation 6 36354207
2021 Septin 4 controls CCNB1 stabilization via APC/CCDC20 during meiotic G2/M transition in mouse oocytes. Journal of cellular physiology 6 34189751
2024 AURKA promotes renal cell carcinoma progression via regulation of CCNB1 transcription. Heliyon 5 38655290
2017 Single-cell, single-mRNA analysis of Ccnb1 promoter regulation. Scientific reports 5 28522800
2024 UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1. Journal of animal science and biotechnology 4 38858680
2023 Hsa_circ_0137652 Regulates miR-1205/CCNB1 Axis to Accelerate the Malignancy of Breast Cancer. Molecular biotechnology 4 36807271
2025 Identification of CCNB1 as a biomarker for cellular senescence in hepatocellular carcinoma: a bioinformatics and experimental validation study. Discover oncology 3 40128499
2025 SNRPB/CCNB1 axis promotes hepatocellular carcinoma progression and cisplatin resistance through enhancing lipid metabolism reprogramming. Journal of experimental & clinical cancer research : CR 3 40682115
2024 Kielin/chordin-like protein deficiency aggravates pressure overload-induced cardiac dysfunction and remodeling via P53/P21/CCNB1 signaling in mice. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 3 38421300
2023 The role of CXCL8 and CCNB1 in predicting hepatocellular carcinoma in the context of cirrhosis: implications for early detection and immune-based therapies. Journal of cancer research and clinical oncology 3 37391641
2022 RAD6 Positively Affects Tumorigenesis of Esophageal Squamous Cell Carcinoma by Regulating Histone Ubiquitination of CCNB1. Biological procedures online 3 35321657
2022 MM-CCNB: Essential protein prediction using MAX-MIN strategies and compartment of common neighboring approach. Computer methods and programs in biomedicine 3 36427433
2000 Localization of new, microdissection- generated, anonymous markers and of the genes Pcsk1, Dhfr, Ndub13, and Ccnb1 to rat chromosome region 2q1. Cytogenetics and cell genetics 3 10773684
2024 Alternative cleavage and polyadenylation of the Ccnb1 mRNA defines accumulation of cyclin protein during the meiotic cell cycle. Nucleic acids research 2 38048302
2024 CCNB1 may as a biomarker for the adipogenic differentiation of adipose-derived stem cells in the postoperative fat transplantation of breast cancer. Gland surgery 2 38323233
2022 Differential expression of cyclins CCNB1 and CCNG1 is involved in the chondrocyte damage of kashin-beck disease. Frontiers in genetics 2 36588792
2021 [Retracted] MicroRNA‑718 serves a tumor‑suppressive role in non‑small cell lung cancer by directly targeting CCNB1. International journal of molecular medicine 2 34296291
2026 CircDCAF6 Modulates Proliferation and Apoptosis in Bovine Myoblasts Through the miR-181d/CCNB1 Axis. Probiotics and antimicrobial proteins 1 41518519
2025 Knockdown of CCNB1 alleviates high glucose-triggered trophoblast dysfunction during gestational diabetes via Wnt/β-catenin signaling pathway. Open medicine (Warsaw, Poland) 1 39822992
2025 Ropivacaine-loaded tumor-derived vesicles targeting CCNB1 and autophagy in nasopharyngeal carcinoma. Materials today. Bio 1 41122232
2023 DEPDC1B enhances malignant phenotypes of multiple myeloma through upregulating CCNB1 and inhibiting p53 signaling pathway. Tissue & cell 1 37979396
2021 Deciphering the modes of human separase inhibition by securin and CDK1-CCNB1. Molecular & cellular oncology 1 34616878
2011 Silencing of Her2, CCNB1 and PKC Genes by siRNA Results in Prolonged Retardation of Neuroblastoma Cell Division. Acta naturae 1 22649691
2010 [The CCNB1, HER2, and PKC silencing induced by small interfering RNA decreases a division of different human cancer cells with interfering RNA decreases a different efficiency]. Molekuliarnaia biologiia 1 20198864
2026 AR-mediated YBX3 suppresses oocyte meiotic resumption in polycystic ovary syndrome by attenuating Pde3A and Ccnb1 expression. Journal of ovarian research 0 41673703
2026 Targeting CCNB1 Repressed Cartilage Degradation Induced by Inflammation Through NF-κB Pathway in Osteoarthritis. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 0 41731977
2026 Multi-omics analysis identifies CCNB1 as a cell cycle factor driving glioblastoma progression and its inhibition by resveratrol. PloS one 0 41838760
2026 Whole transcriptome sequencing identifies key characteristics and potential therapeutic efficacy biomarkers CD1B, CD247, and CCNB1 in cured tuberculosis patients. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 0 41871736
2026 MAPK1 phosphorylates CCNB1 at Ser128 to drive mitotic progression and cell proliferation in triple-negative breast cancer. Life sciences 0 41905453
2025 MND1 Promotes the Proliferation of Prostate Cancer Cell Via the CCNB1/p53 Signaling Pathway. Current cancer drug targets 0 40353402
2025 HROB Induces Lung Adenocarcinoma Progression via ZC3HC1-CCNB1 Axis Regulation and Cell Cycle Dysregulation. Cancer science 0 40654113
2025 Retraction: LINC00346 Acts as a competing endogenous RNA regulating development of hepatocellular carcinoma via modulating CDK1/CCNB1 Axis. Frontiers in bioengineering and biotechnology 0 41280647
2025 The mechanism of luteolin suppressing pancreatic cancer (PC) via cyclin B1 (CCNB1)-mediated signalling. Scientific reports 0 41298459
2024 The role of GADD45G methylation in endometrial cancer: Insights into CDK1/CCNB1 activation and therapeutic opportunities. Journal of cancer research and therapeutics 0 39206984
2023 Hsa-circ-0006091 modulates the proliferation of hepatocellular carcinoma via the miR-622/CCNB1 axis. Turkish journal of medical sciences 0 38813026
2022 CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1 [Retraction]. OncoTargets and therapy 0 36275181
2022 miR-144 negatively regulates the effect of CCNB1 protein on the biological behavior of hepatoma cells. Cellular and molecular biology (Noisy-le-Grand, France) 0 36495514