Affinage

MIF

Macrophage migration inhibitory factor · UniProt P14174

Round 2 corrected
Length
115 aa
Mass
12.5 kDa
Annotated
2026-04-28
130 papers in source corpus 30 papers cited in narrative 29 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Insufficient on-target evidence to synthesize a narrative — discovery timeline does not match the canonical MIF protein.

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.

Evidence

Reading pass · 29 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1989 MIF was molecularly cloned from a lectin-stimulated T-cell hybridoma cDNA library via functional expression cloning in COS-1 cells, encoding a 115-amino acid, ~12 kDa polypeptide that is secreted despite lacking a classical signal sequence and exhibits macrophage migration inhibitory activity. Functional expression cloning, insertional mutagenesis, gel elution of bioactivity Proceedings of the National Academy of Sciences of the United States of America High 2552447
1994 Recombinant mouse and human MIF (12.5 kDa monomer) fold into a highly ordered 3D structure with significant β-sheet and α-helix content as revealed by circular dichroism; recombinant MIF inhibits monocyte migration, induces TNF-α secretion, and stimulates nitric oxide production by IFN-γ-primed macrophages, with no significant post-translational glycosylation detected. Recombinant protein purification, circular dichroism spectroscopy, guanidine HCl unfolding, macrophage migration assay, ELISA, NO assay Biochemistry High 7947826
1995 The mouse MIF gene spans ~1 kb on chromosome 10, shares high structural homology with human MIF, and its promoter contains both inflammatory/growth factor response elements and peptide hormone-associated regulatory motifs; two MIF pseudogenes were also identified. Genomic cloning, Southern blot, chromosomal mapping, promoter sequence analysis Journal of immunology Medium 7706726
1997 MIF is released from macrophages and T lymphocytes upon glucocorticoid stimulation and, once secreted, counteracts the inhibitory effects of glucocorticoids on TNF-α, IL-1β, IL-6, and IL-8 production in LPS-stimulated monocytes in vitro and antagonizes glucocorticoid inhibition of T-cell proliferation by restoring IL-2 and IFN-γ production. Cell stimulation assays, cytokine ELISA, T-cell proliferation assays, in vivo endotoxemia models Critical reviews in immunology High 29773026 9034724
1999 MIF functionally inactivates p53 tumor suppressor activity: MIF cDNAs were isolated in screens bypassing p53-mediated growth arrest or apoptosis, and MIF treatment overcame p53 activity in three biological assays and suppressed p53 transcriptional activation. Functional genetic screens (bypass of growth arrest/apoptosis), reporter assays, cell growth assays The Journal of experimental medicine High 10562313
2000 MIF specifically interacts with the intracellular protein Jab1 (CSN5), a coactivator of AP-1 transcription; MIF colocalizes with Jab1 in the cytosol, and exogenously added MIF enters cells by endocytosis to bind Jab1. MIF inhibits Jab1-enhanced AP-1 activity, blocks Jab1-mediated JNK activation and c-Jun phosphorylation, and increases p27Kip1 expression by stabilizing p27Kip1 protein, thereby antagonizing Jab1-dependent cell cycle progression. Amino acids 50–65 and Cys-60 of MIF are critical for Jab1 binding. Co-immunoprecipitation, colocalization (confocal), endocytosis tracking, AP-1 reporter assays, JNK kinase assay, Western blot (p27Kip1 stabilization), mutagenesis Nature High 11089976
2000 GIF/MIF undergoes two sequential post-translational modifications in human suppressor T cell hybridomas: cysteinylation at Cys-60 and phosphorylation at Ser-91. Cysteinylated GIF/MIF (but not wild-type) possesses immunosuppressive activity on IgE responses and high affinity for GIF receptors; in vitro treatment of recombinant MIF with cystine preferentially cysteinylates Cys-60 and confers equivalent bioactivity. Affinity purification, peptide mapping, mass spectrometry, in vitro cysteinylation, receptor binding assay, IgE response assay Proceedings of the National Academy of Sciences of the United States of America High 11069294
2002 MIF secretion is induced by cell adhesion to fibronectin in quiescent fibroblasts via protein kinase C-dependent release; autocrine MIF subsequently promotes integrin-dependent MAP kinase activation, cyclin D1 expression, and DNA synthesis. MIF-deficient cells show higher retinoblastoma protein activity and lower E2F transcriptional activity. Fibronectin adhesion assay, neutralizing antibodies, PKC inhibition, MAP kinase assay, cyclin D1 Western blot, BrdU incorporation, Rb/E2F reporter assays The Journal of biological chemistry High 12297513
2003 CD74 (invariant chain) is the high-affinity cell surface receptor for MIF: MIF binds the extracellular domain of CD74 with Kd ~9 nM (by surface plasmon resonance), and CD74 is required for MIF-induced ERK1/2 MAP kinase activation, cell proliferation, and PGE2 production. Soluble CD74 ectodomain inhibits MIF-mediated ERK activation. Expression cloning, surface plasmon resonance (BIAcore), co-immunoprecipitation, siRNA knockdown, ERK phosphorylation assay, PGE2 ELISA, proliferation assay The Journal of experimental medicine High 12782713
2006 CD44 is an essential signaling component of the MIF-CD74 receptor complex: CD74 alone mediates MIF binding, but MIF-induced ERK1/2 phosphorylation requires coexpression of full-length CD44. MIF binding triggers serine phosphorylation of both CD74 and CD44; ERK activation through CD44 requires Src tyrosine kinase activity. CD74 and CD44 are both necessary for MIF-mediated protection from apoptosis. Stable transfection in CD74/CD44-deficient COS-7/M6 cells, co-immunoprecipitation, siRNA, kinase inhibitors, ERK phosphorylation assay, apoptosis assay Immunity High 17045821
2007 MIF functions as a non-canonical ligand for chemokine receptors CXCR2 and CXCR4, triggering Gαi- and integrin-dependent monocyte/T-cell arrest and chemotaxis, rapid integrin activation, and calcium influx. MIF competes with canonical CXCR4 ligands, directly binds CXCR2, and CXCR2 forms a receptor complex with CD74. In vivo, MIF-induced leukocyte recruitment requires IL8rb (Cxcr2), and MIF deficiency impairs monocyte adhesion in atherosclerotic arteries. Chemotaxis/arrest assays, calcium flux, integrin activation assay, competitive binding, co-immunoprecipitation (CXCR2/CD74 complex), Mif-knockout and Il8rb-knockout mice, in vivo atherosclerosis model Nature medicine High 17435771
2008 MIF is released from the ischemic heart and stimulates AMPK activation through CD74, promoting glucose uptake and protecting against ischemia-reperfusion injury. Germline Mif deletion impairs ischemic AMPK signaling; human fibroblasts with low-activity MIF promoter polymorphisms show diminished MIF release and AMPK activation during hypoxia. Mouse cardiac ischemia-reperfusion model, Mif-knockout mice, AMPK activity assay, glucose uptake assay, CD74 blocking antibody, human fibroblast hypoxia experiments, MIF promoter genotyping Nature High 18235500
2009 CD74 forms functional heteromeric receptor complexes with CXCR4 at the cell surface (confirmed by co-immunoprecipitation from endogenous monocytes). MIF-stimulated, CD74-dependent AKT activation is blocked by anti-CXCR4 antibodies and AMD3100, but CXCL12-stimulated AKT activation is not reduced by anti-CD74, indicating MIF-specific signaling through the CD74/CXCR4 complex. Co-immunoprecipitation (HEK293 transfectants and endogenous monocytes), surface expression analysis, AKT phosphorylation assay, antibody/small molecule blockade FEBS letters High 19665027
2009 MIF-induced neutrophil accumulation in the alveolar space occurs via CD74 expressed on alveolar macrophages: MIF activates p44/p42 MAPK in macrophages through CD74, leading to chemokine (MIP-2, KC) release that recruits neutrophils. Anti-CD74 antibody inhibits MIF-induced MAPK phosphorylation, chemokine release, and neutrophil accumulation in vivo. Intratracheal instillation of recombinant MIF, anti-CD74 antibody blockade, BAL fluid analysis, MAPK phosphorylation assay, ELISA, MIF inhibitor (ISO-1) Respiratory research High 19413900
2011 D-dopachrome tautomerase (D-DT/MIF-2), encoded by a gene adjacent to MIF, binds CD74 with high affinity, activates ERK1/2 MAP kinase and downstream pro-inflammatory pathways, and its immunoneutralization protects mice from lethal endotoxemia; D-DT functions as a second MIF-like cytokine ligand for CD74. Recombinant protein binding assay, ERK1/2 phosphorylation assay, in vivo endotoxemia model with neutralizing antibodies, cytokine ELISA Proceedings of the National Academy of Sciences of the United States of America High 21817065
2012 MIF promotes survival and proliferation of neural stem/progenitor cells (NSPCs) via CD74-CD44 receptor signaling; MIF increases phosphorylation of Akt, Erk, AMPK, and Stat3, and upregulates Hes3 and Egfr. MIF shRNA knockdown increases caspase 3/7 activity in neurospheres. MIF also acts as a chemoattractant for NSPCs. Neurosphere formation assay, retroviral shRNA knockdown, MIF inhibitor (ISO-1), flow cytometry, phosphoprotein assays, caspase activity assay, chemotaxis assay Journal of cell science Medium 22454509
2014 MIF promotes invasive synoviocyte phenotype via the CD74/CD44 receptor complex: MIF increases CD44 expression in synovial fibroblasts, promotes its recruitment into the signal transduction complex, and stimulates alternative CD44 exon splicing producing oncogenic CD44v3-v6 isoforms. Downstream MAPK and RhoA signaling and invasion require both MIF and CD74 and are reduced by MIF pathway antagonists. Co-immunoprecipitation, alternative splicing analysis, MAPK/RhoA activation assays, invasion assay (Matrigel), siRNA, MIF pathway antagonists, synoviocytes from RA patients and MIF allele carriers Proceedings of the National Academy of Sciences of the United States of America High 27872288
2014 MIF gene transcription is regulated by the transcription factor ICBP90 (UHRF1) through direct interaction with the -794 CATT microsatellite promoter region. ICBP90 is essential for MIF transcription in monocytes/macrophages, B and T lymphocytes, and synovial fibroblasts; TLR-induced MIF transcription is regulated in an ICBP90- and CATT-repeat-length-dependent manner. Oligonucleotide affinity chromatography, LC-MS/MS, shRNA knockdown, promoter reporter assays, genome-wide transcription profiling, ChIP The Journal of clinical investigation High 26752645
2016 MIF-CD74 signaling inhibits IFN-γ secretion in microglia through phosphorylation of ERK1/2, promoting a pro-tumorigenic M2 microglial state. Antibody neutralization or siRNA-mediated inhibition of MIF or CD74 reinstates IFN-γ release, induces M2-to-M1 shift, and prolongs survival in glioma-implanted mice. siRNA knockdown, neutralizing antibody, ERK1/2 phosphorylation assay, IFN-γ ELISA, microglial polarization assays, in vivo glioma implantation model Oncogene High 27157615
2016 MIF is a PARP-1-dependent, AIF-associated nuclease (PAAN): AIF recruits MIF to the nucleus where MIF cleaves genomic DNA into large fragments. Depletion of MIF, disruption of the AIF-MIF interaction, or mutation of Glu-22 in MIF's catalytic nuclease domain blocks nuclease activity and inhibits chromatinolysis, glutamate excitotoxicity-induced cell death, and focal stroke damage. Co-immunoprecipitation (AIF-MIF), nuclear fractionation, DNA cleavage assay, site-directed mutagenesis (E22), siRNA knockdown, mouse stroke model, glutamate excitotoxicity assay Science High 27846469
2017 MIF interacts with the serine protease HTRA1 and inhibits its proteolytic activity, representing the first endogenous inhibitor identified for HTRA1. Both proteins co-localize in astrocytes, and the MIF-HTRA1 interaction modulates availability of growth factors and extracellular matrix molecules affecting astrocytic cell growth and differentiation. Co-immunoprecipitation, protease activity assay, colocalization (immunofluorescence), functional rescue of HTRA1 substrates Cellular and molecular life sciences Medium 28726057
2018 Soluble CD74 ectodomain (sCD74) co-treated with MIF reroutes MIF signaling from pro-survival AKT activation to necroptosis in cardiac myofibroblasts via RIP1/RIP3-dependent pathways. sCD74 diminishes MIF-mediated AKT activation and triggers p38 activation, enabling CD74-dependent necroptotic processes specifically in cardiac fibroblasts but not cardiomyocytes. Cardiac fibroblast isolation, recombinant protein co-treatment, Western blot (AKT, p38, RIP1/RIP3), microarray, cell death assay, gene expression analysis Journal of the American Heart Association Medium 30371153
2018 EC-derived MIF decreases pericyte contractility by reducing phosphorylated myosin light chain, increasing intercellular PC gap formation, and enhancing neutrophil transmigration across the EC-PC barrier. EC-specific MIF knockout mice show decreased neutrophil infiltration to BAL and increased myosin light-chain phosphorylation in pericytes in acute lung injury. EC-specific MIF knockout mouse, micro-ELISA, RNA-seq, flow cytometry, 2D contractility assay on silicone substrates, in vitro microvascular model, BAL neutrophil counting FASEB journal High 30252532
2018 Molecular dynamics simulations up to 1 µs revealed allosteric communication between a residue at the MIF solvent channel opening and distal CD74-activating residues in the β-strand region. Experiments validated this allosteric regulatory site and identified a pathway via the MIF β-strands controlling CD74 activation. Molecular dynamics simulation (up to 1 µs), mutagenesis, CD74 activation assay, NMR dynamics Angewandte Chemie Medium 29669180
2021 MIF is a 3' flap nuclease that translocates to the nucleus during S phase, where it co-localizes with PARP-1 at DNA replication forks. MIF nuclease activity is required to resolve replication stress; MIF loss increases mutation frequency, causes cell cycle delays, and inhibits DNA synthesis and cell growth, effects rescued by wild-type but not nuclease-deficient MIF mutant. Nuclear fractionation, co-localization with replication fork markers, in vitro nuclease assay, nuclease-deficient mutant rescue, cell cycle analysis, DNA synthesis assay, tumor growth assay Nature communications High 34012010
2021 A PROTAC (MD13) targeting the MIF tautomerase active site induces near-complete MIF protein degradation at ~100 nM DC50 in A549 cells, suppresses cell proliferation by deactivating the MAPK pathway, and causes G2/M cell cycle arrest. PROTAC degrader design, Western blot (MIF degradation), cell proliferation assay, MAPK pathway analysis, cell cycle FACS, 3D tumor spheroid assay Angewandte Chemie Medium 34018657
2022 HDAC6 inhibition or aspirin treatment promotes MIF acetylation at K78 residue (identified by mass spectrometry). MIF K78 acetylation suppresses the MIF-AIF interaction and impairs MIF translocation to the nucleus in ischemic cortical neurons, reducing DNA fragmentation and neuronal death. MIF K78Q mutant knock-in mice show reduced infarct size after stroke. Mass spectrometry (PTM identification), co-immunoprecipitation (MIF-AIF), nuclear fractionation, HDAC6 inhibitor/aspirin treatment, MIF K78Q knock-in mouse, TUNEL assay, infarct volume measurement Cell death & disease High 35585040
2024 CD74 is a functional MIF receptor on activated human CD4+ T cells: resting T cells express CD74 intracellularly, but activation upregulates CD74 surface expression with chondroitin sulfate modification. Proximity ligation assay demonstrates CD74/CXCR4 heterocomplexes on activated CD4+ T cells that are reduced after MIF treatment (suggesting MIF-mediated internalization). MIF-induced CD4+ T cell migration requires both CD74 and CXCR4. Flow cytometry, Western blot, immunohistochemistry, RNA-seq re-analysis, proteomics, 3D-matrix live cell imaging, receptor-specific inhibitors, proximity ligation assay (PLA), COVID-19 patient cohort Cellular and molecular life sciences High 38992165
2024 MIF directly binds PINK1 and disrupts the PINK1-Parkin protein-protein interaction, impairing Parkin recruitment to mitochondria and blocking initiation of PINK1-Parkin-dependent mitophagy in renal tubular epithelial cells during sepsis-associated AKI. MIF inhibition (ISO-1) or mitophagy activation reverses this apoptosis. Co-immunoprecipitation (MIF-PINK1), mitophagy assay, Parkin translocation assay, MIF overexpression/knockdown, ISO-1 inhibitor, apoptosis assay Cell death & disease Medium 38956064

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Towards a proteome-scale map of the human protein-protein interaction network. Nature 2090 16189514
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2003 Macrophage migration inhibitory factor: a regulator of innate immunity. Nature reviews. Immunology 1469 14502271
2007 MIF is a noncognate ligand of CXC chemokine receptors in inflammatory and atherogenic cell recruitment. Nature medicine 1058 17435771
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2003 MIF signal transduction initiated by binding to CD74. The Journal of experimental medicine 935 12782713
2004 Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nature biotechnology 916 15592455
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2008 Large-scale proteomics and phosphoproteomics of urinary exosomes. Journal of the American Society of Nephrology : JASN 607 19056867
2006 CD44 is the signaling component of the macrophage migration inhibitory factor-CD74 receptor complex. Immunity 556 17045821
1999 A proinflammatory cytokine inhibits p53 tumor suppressor activity. The Journal of experimental medicine 533 10562313
2000 Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1. Nature 489 11089976
2003 Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nature biotechnology 485 12665801
2011 Genome-wide association study identifies loci influencing concentrations of liver enzymes in plasma. Nature genetics 456 22001757
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2015 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell 433 26638075
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
1994 Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF). Biochemistry 378 7947826
2008 Macrophage migration inhibitory factor stimulates AMP-activated protein kinase in the ischaemic heart. Nature 376 18235500
2020 A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Nature biotechnology 355 33046875
2016 A nuclease that mediates cell death induced by DNA damage and poly(ADP-ribose) polymerase-1. Science (New York, N.Y.) 334 27846469
1989 Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor. Proceedings of the National Academy of Sciences of the United States of America 316 2552447
2002 A functional promoter polymorphism in the macrophage migration inhibitory factor (MIF) gene associated with disease severity in rheumatoid arthritis. Genes and immunity 314 12070782
2013 Endogenous purification reveals GREB1 as a key estrogen receptor regulatory factor. Cell reports 307 23403292
2014 Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity. The Plant journal : for cell and molecular biology 235 24888433
2021 Macrophage migration inhibitory factor (MIF): A multifaceted cytokine regulated by genetic and physiological strategies. Pharmacology & therapeutics 222 34673115
2019 The immunobiology of MIF: function, genetics and prospects for precision medicine. Nature reviews. Rheumatology 209 31197253
2009 A functional heteromeric MIF receptor formed by CD74 and CXCR4. FEBS letters 199 19665027
2011 The D-dopachrome tautomerase (DDT) gene product is a cytokine and functional homolog of macrophage migration inhibitory factor (MIF). Proceedings of the National Academy of Sciences of the United States of America 198 21817065
2010 Macrophage migration inhibitory factor (MIF): a promising biomarker. Drug news & perspectives 197 20520854
2019 Evolving complexity of MIF signaling. Cellular signalling 196 30682543
2000 Macrophage migration inhibitory factor (MIF): its essential role in the immune system and cell growth. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 172 11032394
2012 D-dopachrome tautomerase (D-DT or MIF-2): doubling the MIF cytokine family. Cytokine 148 22507380
1997 Macrophage migration inhibitory factor (MIF): a glucocorticoid counter-regulator within the immune system. Critical reviews in immunology 143 9034724
2020 MIF-Dependent Control of Tumor Immunity. Frontiers in immunology 136 33324425
2019 Macrophage migration inhibitory factor (MIF) as a therapeutic target for rheumatoid arthritis and systemic lupus erythematosus. Expert opinion on therapeutic targets 127 31414920
2009 Dual role of macrophage migration inhibitory factor (MIF) in human breast cancer. BMC cancer 126 19602265
2003 Macrophage migration inhibitory factor (MIF): Its potential role in tumor growth and tumor-associated angiogenesis. Annals of the New York Academy of Sciences 115 12814949
2016 MIF-CD74 signaling impedes microglial M1 polarization and facilitates brain tumorigenesis. Oncogene 114 27157615
2004 Mechanisms and effectors of MIF-dependent promotion of tumourigenesis. Cellular signalling 113 14607271
2020 Exosomes from mesenchymal stem cells overexpressing MIF enhance myocardial repair. Journal of cellular physiology 109 31960418
2008 Role of MIF in inflammation and tumorigenesis. Oncology 109 18791328
2006 Dual regulation of macrophage migration inhibitory factor (MIF) expression in hypoxia by CREB and HIF-1. Biochemical and biophysical research communications 109 16854377
2015 A Secreted MIF Cytokine Enables Aphid Feeding and Represses Plant Immune Responses. Current biology : CB 105 26119751
2009 Macrophage CD74 contributes to MIF-induced pulmonary inflammation. Respiratory research 104 19413900
2008 The MIF receptor CD74 in diabetic podocyte injury. Journal of the American Society of Nephrology : JASN 101 18842989
2000 Enhancement of macrophage migration inhibitory factor (MIF) expression in injured epidermis and cultured fibroblasts. Biochimica et biophysica acta 95 10564712
2019 MIF family proteins in genitourinary cancer: tumorigenic roles and therapeutic potential. Nature reviews. Urology 92 30914802
2012 Macrophage migration inhibitory factor (MIF) promotes cell survival and proliferation of neural stem/progenitor cells. Journal of cell science 92 22454509
1995 Cloning and characterization of the gene for mouse macrophage migration inhibitory factor (MIF). Journal of immunology (Baltimore, Md. : 1950) 92 7706726
2011 Macrophage migration inhibitory factor (MIF) expression in human malignant gliomas contributes to immune escape and tumour progression. Acta neuropathologica 76 21773885
2016 MIF allele-dependent regulation of the MIF coreceptor CD44 and role in rheumatoid arthritis. Proceedings of the National Academy of Sciences of the United States of America 75 27872288
2019 Biological roles and an evolutionary sketch of the GRF-GIF transcriptional complex in plants. BMB reports 74 30885290
2011 Macrophage migration inhibitory factor (MIF): a key player in protozoan infections. International journal of biological sciences 73 22110378
2005 Role of macrophage migration inhibitory factor (MIF) in the skin. Journal of dermatological science 68 15659324
2011 MIF: a key player in cutaneous biology and wound healing. Experimental dermatology 65 21158933
2012 MIF, MIF alleles, and prospects for therapeutic intervention in autoimmunity. Journal of clinical immunology 63 22968741
2019 A MIF-like effector suppresses plant immunity and facilitates nematode parasitism by interacting with plant annexins. Journal of experimental botany 62 31365744
2008 Chemokine-like functions of MIF in atherosclerosis. Journal of molecular medicine (Berlin, Germany) 62 18385967
2006 Insight into the biology of macrophage migration inhibitory factor (MIF) revealed by the cloning of its cell surface receptor. Cell research 61 16474429
2017 Macrophage Migration Inhibitory Factor (MIF): A Glucocorticoid Counter-Regulator within the Immune System. Critical reviews in immunology 56 29773026
2012 Neutralization of macrophage migration inhibitory factor (MIF) by fully human antibodies correlates with their specificity for the β-sheet structure of MIF. The Journal of biological chemistry 56 22238348
2019 Role of Macrophage Migration Inhibitory Factor (MIF) in Melanoma. Cancers 54 31013837
2018 Soluble CD74 Reroutes MIF/CXCR4/AKT-Mediated Survival of Cardiac Myofibroblasts to Necroptosis. Journal of the American Heart Association 54 30371153
2016 Transcription factor ICBP90 regulates the MIF promoter and immune susceptibility locus. The Journal of clinical investigation 52 26752645
2001 Neuroendocrine properties of macrophage migration inhibitory factor (MIF). Immunology and cell biology 52 11488984
2015 MIF and CXCL12 in Cardiovascular Diseases: Functional Differences and Similarities. Frontiers in immunology 50 26257740
2013 Platelets are a previously unrecognised source of MIF. Thrombosis and haemostasis 50 23846621
2001 Macrophage migration inhibitory factor (MIF) modulates innate immune responses induced by endotoxin and Gram-negative bacteria. Journal of endotoxin research 49 11753217
2006 Tumor-derived macrophage migration inhibitory factor (MIF) inhibits T lymphocyte activation. Cytokine 47 16522371
2002 Adhesion-dependent signaling by macrophage migration inhibitory factor (MIF). The Journal of biological chemistry 47 12297513
2000 Posttranslational modification of the glycosylation inhibiting factor (GIF) gene product generates bioactive GIF. Proceedings of the National Academy of Sciences of the United States of America 47 11069294
2020 GRF-GIF Chimeras Boost Plant Regeneration. Trends in plant science 46 33349565
2014 Hypoxia signaling regulates macrophage migration inhibitory factor (MIF) expression in stroke. Molecular neurobiology 46 24826917
2009 Inhibition of MIF leads to cell cycle arrest and apoptosis in pancreatic cancer cells. The Journal of surgical research 46 19726058
2006 Transgenic mice overexpressing macrophage migration inhibitory factor (MIF) exhibit high-turnover osteoporosis. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 46 16753018
1995 Expression of growth inhibitory factor (GIF) in normal and injured rat brains. Neurochemistry international 46 7655350
2016 The non-mammalian MIF superfamily. Immunobiology 45 27780588
2020 The Role of MIF on Eosinophil Biology and Eosinophilic Inflammation. Clinical reviews in allergy & immunology 44 30680604
2014 Down-regulation of MIF by NFκB under hypoxia accelerated neuronal loss during stroke. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 43 24970391
2024 CD74 is a functional MIF receptor on activated CD4+ T cells. Cellular and molecular life sciences : CMLS 42 38992165
2021 Proteolysis Targeting Chimera (PROTAC) for Macrophage Migration Inhibitory Factor (MIF) Has Anti-Proliferative Activity in Lung Cancer Cells. Angewandte Chemie (International ed. in English) 42 34018657
2013 Deletion of CD74, a putative MIF receptor, in mice enhances osteoclastogenesis and decreases bone mass. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 41 23044992
2014 MIF family members cooperatively inhibit p53 expression and activity. PloS one 38 24932684
2018 Nanosecond Dynamics Regulate the MIF-Induced Activity of CD74. Angewandte Chemie (International ed. in English) 37 29669180
2019 A selective small-molecule inhibitor of macrophage migration inhibitory factor-2 (MIF-2), a MIF cytokine superfamily member, inhibits MIF-2 biological activity. The Journal of biological chemistry 36 31578280
2004 Cysteine-dependent immune regulation by TRX and MIF/GIF family proteins. Immunology letters 36 15081538
2016 Predicted structure of MIF/CD74 and RTL1000/CD74 complexes. Metabolic brain disease 35 26851955
2016 CHD7 promotes proliferation of neural stem cells mediated by MIF. Molecular brain 34 27955690
2001 Sharable representation of clinical guidelines in GLIF: relationship to the Arden Syntax. Journal of biomedical informatics 34 11723699
2020 The role of MIF in chronic lung diseases: looking beyond inflammation. American journal of physiology. Lung cellular and molecular physiology 33 32208924
2023 Obacunone targets macrophage migration inhibitory factor (MIF) to impede osteoclastogenesis and alleviate ovariectomy-induced bone loss. Journal of advanced research 32 36657717
2021 MIF is a 3' flap nuclease that facilitates DNA replication and promotes tumor growth. Nature communications 31 34012010
2018 Endothelial cell-secreted MIF reduces pericyte contractility and enhances neutrophil extravasation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 31 30252532
2018 Macrophage Migration Inhibitory Factor (MIF) Drives Murine Psoriasiform Dermatitis. Frontiers in immunology 29 30333830
2007 From MIF-1 to endomorphin: the Tyr-MIF-1 family of peptides. Peptides 29 17988762
2021 Isopsoralen ameliorates rheumatoid arthritis by targeting MIF. Arthritis research & therapy 28 34535196
2015 4-IPP, a selective MIF inhibitor, causes mitotic catastrophe in thyroid carcinomas. Endocrine-related cancer 28 26206776
1998 Brain injury and growth inhibitory factor (GIF)--a minireview. Neurochemical research 28 9482244
2022 The IL-25-dependent tuft cell circuit driven by intestinal helminths requires macrophage migration inhibitory factor (MIF). Mucosal immunology 27 35288645
2019 Macrophage Migration Inhibitory Factor (MIF) Expression Increases during Myocardial Infarction and Supports Pro-Inflammatory Signaling in Cardiac Fibroblasts. Biomolecules 26 30678084
2022 Macrophage migration inhibitory factor (MIF) acetylation protects neurons from ischemic injury. Cell death & disease 25 35585040
2021 The Role of Macrophage Migration Inhibitory Factor (MIF) in Asthmatic Airway Remodeling. Allergy, asthma & immunology research 25 33191679
1996 Patterns of growth inhibitory factor (GIF) and glial fibrillary acidic protein relative level changes differ following left middle cerebral artery occlusion in rats. Brain research 24 8869568
2021 Inhibition of macrophage migration inhibitory factor (MIF) as a therapeutic target in bleomycin-induced pulmonary fibrosis rats. American journal of physiology. Lung cellular and molecular physiology 23 33881353
2018 Circulating macrophage migration inhibitory factor (MIF) in patients with heart failure. Cytokine 23 29723777
2005 Glucocorticoids suppress macrophage migration inhibitory factor (MIF) expression in a cell-type-specific manner. Journal of molecular endocrinology 23 15821118
2020 Immune modulation by the macrophage migration inhibitory factor (MIF) family: D-dopachrome tautomerase (DDT) is not (always) a backup system. Cytokine 22 32417648
2017 Invariant Chain Complexes and Clusters as Platforms for MIF Signaling. Cells 22 28208600
2017 Macrophage migration inhibitory factor (MIF) modulates trophic signaling through interaction with serine protease HTRA1. Cellular and molecular life sciences : CMLS 22 28726057
2014 Exploring the binding mechanisms of MIF to CXCR2 using theoretical approaches. Physical chemistry chemical physics : PCCP 22 25526079
2009 Conservation and variation of the parapoxvirus GM-CSF-inhibitory factor (GIF) proteins. The Journal of general virology 22 19264672
2000 Macrophage migration inhibitory factor (MIF) as a paracrine mediator in the interaction of testicular somatic cells. Andrologia 22 10702866
2009 Increase of macrophage migration inhibitory factor (MIF) expression in cardiomyocytes during chronic hypoxia. Clinica chimica acta; international journal of clinical chemistry 21 19394321
2002 Human cytomegalovirus-mediated induction of MIF in fibroblasts. Virology 21 12167338
2024 Use of GRF-GIF chimeras and a ternary vector system to improve maize (Zea mays L.) transformation frequency. The Plant journal : for cell and molecular biology 20 38923048
2024 Macrophage migration inhibitory factor (MIF) suppresses mitophagy through disturbing the protein interaction of PINK1-Parkin in sepsis-associated acute kidney injury. Cell death & disease 20 38956064
2021 Macrophage migration inhibitory factor (MIF) enhances hypochlorous acid production in phagocytic neutrophils. Redox biology 20 33823474
2013 Gastric intrinsic factor deficiency with combined GIF heterozygous mutations and FUT2 secretor variant. Biochimie 20 23402911
2011 MIF from mussel: coding sequence, phylogeny, polymorphism, 3D model and regulation of expression. Developmental and comparative immunology 20 22085783
2012 Candidate gene case-control and functional study shows macrophage inhibitory factor (MIF) polymorphism is associated with cutaneous leishmaniasis. Cytokine 19 23068083
1998 Neuroimmunomodulation by macrophage migration inhibitory factor (MIF). Annals of the New York Academy of Sciences 19 9629239