Affinage

YBX1

Y-box-binding protein 1 · UniProt P67809

Length
324 aa
Mass
35.9 kDa
Annotated
2026-04-28
100 papers in source corpus 49 papers cited in narrative 49 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

YBX1 is a multifunctional cold-shock domain (CSD) protein that serves as a central post-transcriptional regulator of mRNA fate—stabilizing, translationally activating or repressing, and alternatively splicing target transcripts—while also participating in DNA damage repair, cytoskeletal organization, and exosomal RNA sorting. At low stoichiometric ratios YBX1 binds mRNA as a monomer and stabilizes transcripts by competing with eIF4E at the 5′ cap, whereas at high ratios it oligomerizes into ~700 kDa mRNP particles that repress translation; Akt-mediated phosphorylation at Ser102 relieves this cap-dependent repression, and acetylation at Lys81 blocks 5′UTR binding to oncogenic targets including HIF1A, MYC, and G3BP1 (PMID:11574481, PMID:15494450, PMID:16354698, PMID:31668005, PMID:34465866). YBX1 promotes exon inclusion at weak 3′ splice sites by recruiting U2AF65 to polypyrimidine tracts, undergoes liquid–liquid phase separation to sort miRNAs and small ncRNAs into exosomes, and facilitates stress granule dynamics through ATP-independent RNA unwinding (PMID:22730292, PMID:34766549, PMID:34469566). Its subcellular localization is cell-cycle regulated—transportin-1-mediated nuclear import during late G2/M is enabled by dephosphorylation at S102/S165/S176—and compartment-specific functions range from nuclear transcriptional repression (e.g., Col1a1 promoter, CDK inhibitors) to cytoplasmic mRNA stabilization (Col1a1 mRNA, Smad2 protein) and centrosomal roles in microtubule nucleation and cytokinesis (PMID:27794479, PMID:32013098, PMID:27591085, PMID:15899865, PMID:32882852).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 1995 High

    Establishing that YBX1 preferentially binds single-stranded DNA and can induce/stabilize single-stranded regions at gene promoters answered how a cold-shock domain protein could repress transcription without conventional sequence-specific dsDNA recognition.

    Evidence EMSA, methylation interference, and mung bean nuclease assays with purified recombinant YB-1 at the MHC class II DRA promoter

    PMID:7876087

    Open questions at the time
    • Full-genome binding site repertoire unknown
    • Whether ssDNA melting is catalytic or stoichiometric was not resolved
    • Relevance to endogenous transcriptional repression in vivo not shown
  2. 2001 High

    Three concurrent discoveries established YBX1's core cytoplasmic activities: cap-dependent mRNA stabilization via CSD-eIF4E competition, rapid nucleic acid annealing/strand exchange modulated by phosphorylation, and a latent-to-active conversion model in which mRNA-bound cytoplasmic YBX1 is proteolytically released for nuclear gene activation.

    Evidence In vitro mRNA stability and cap-binding assays (PMID:11574481); nucleic acid annealing reconstitution (PMID:11585833); oligo-dT fractionation, RNase liberation, and thrombin-stimulated reporter assays in endothelial cells (PMID:11390977)

    PMID:11390977 PMID:11574481 PMID:11585833

    Open questions at the time
    • Identity of the protease that cleaves YBX1 in vivo was not determined
    • Whether cap competition and strand exchange use the same CSD surface was unknown
  3. 2004 High

    Demonstrating concentration-dependent oligomerization of YBX1 on mRNA (monomer at polysomal ratios, ~20-mer at high ratios) provided a physical basis for the switch between translational activation and repression.

    Evidence Atomic force microscopy, electron microscopy, and biochemical sedimentation at defined YBX1:mRNA ratios

    PMID:15494450

    Open questions at the time
    • Structural details of the multimer interface were not resolved
    • How cells control local YBX1:mRNA ratios in vivo was unknown
  4. 2005 High

    Two studies established YBX1's physiological importance and autoregulatory logic: knockout embryos die after E13.5 with premature senescence driven by derepressed CDK inhibitors, while YBX1 autoregulates its own translation via 3′UTR binding that is antagonized by PABP.

    Evidence YBX1-knockout mice with siRNA rescue of CDK inhibitors (PMID:15899865); in vitro translation with UCCAG/ACAA motif mapping and PABP competition (PMID:15798215)

    PMID:15798215 PMID:15899865

    Open questions at the time
    • Whether autoregulatory 3′UTR mechanism operates in all tissues was untested
    • Downstream mediators of the embryonic lethality beyond p16/p21 were not identified
  5. 2006 High

    Identifying Akt as the kinase that phosphorylates YBX1 and relieves its cap-dependent translational repression—while leaving IRES-dependent translation unaffected—linked growth factor signaling directly to YBX1-controlled mRNA translation.

    Evidence In vitro translation assays with Akt-phosphorylated YBX1, Co-IP of Akt with mRNPs, phosphorylation-deficient mutant analysis

    PMID:16354698

    Open questions at the time
    • Whether Akt modifies YBX1 on polysomes or only free mRNPs was unclear
    • Full spectrum of Akt-derepressed mRNAs was not defined
  6. 2007 High

    Demonstrating that YBX1 regulates alternative splicing of CD44 exons in cooperation with PP2Cγ phosphatase expanded its role from translational control to pre-mRNA processing.

    Evidence Co-IP of PP2Cγ–YBX1, in vivo splicing assays, siRNA knockdown affecting CD44 v4/v5 inclusion

    PMID:17572683

    Open questions at the time
    • Whether PP2Cγ directly dephosphorylates YBX1 or acts indirectly was not resolved
  7. 2012 High

    SELEX identification of CAUC/CACC motifs and demonstration that YBX1 facilitates U2AF65 recruitment to weak polypyrimidine tracts defined the molecular mechanism of YBX1-dependent exon inclusion.

    Evidence SELEX, direct YBX1–U2AF65 protein–protein interaction assays, in vivo splicing minigene assays

    PMID:22730292

    Open questions at the time
    • Genome-wide map of YBX1-dependent splicing events was not generated
    • Structural basis of YBX1–U2AF65 interaction was unknown
  8. 2009 High

    Localizing YBX1 to mitochondria and showing that its depletion reduces mitochondrial mismatch-binding and MMR activity extended its DNA repair roles beyond the nucleus.

    Evidence Mitochondrial fractionation, mismatch-binding assays in mitochondrial extracts, siRNA depletion, mitochondrial DNA mutagenesis assay in HeLa cells

    PMID:19272840

    Open questions at the time
    • Whether YBX1 directly recognizes mismatches or acts as a cofactor was not distinguished
    • Import mechanism into mitochondria was not identified
  9. 2013 High

    Showing that YBX1 disrupts nuclear MutSα/PCNA complex formation via PIP-box motifs revealed that YBX1 can inhibit nuclear MMR—opposing its supportive role in mitochondrial MMR—and linked overexpression to microsatellite instability.

    Evidence In vitro MMR reconstitution, PIP-box mutagenesis, Co-IP with PCNA, microsatellite instability and mutation frequency assays

    PMID:24141788

    Open questions at the time
    • How cells prevent simultaneous nuclear MMR inhibition and mitochondrial MMR promotion was unknown
    • Relevance to spontaneous tumorigenesis not shown in animal models
  10. 2015 High

    Multiple 2015 studies collectively established YBX1 as a translational activator of specific oncogenic 5′UTRs (HIF1A, G3BP1), a target of tRNA-fragment-mediated displacement from 3′UTRs, and a substrate for K27-linked ubiquitination by HACE1 for exosomal secretion, vastly broadening its regulatory scope.

    Evidence 5′UTR binding/translational reporter assays and in vivo metastasis models for HIF1A (PMID:25965573) and G3BP1/stress granules (PMID:25800057); tRF displacement from 3′UTRs with LNA/synthetic mimetics in breast cancer (PMID:25957686); HACE1 ubiquitin chain-type analysis and TSG101 Co-IP for secretion (PMID:26343856)

    PMID:25800057 PMID:25957686 PMID:25965573 PMID:26343856

    Open questions at the time
    • Structural basis for 5′UTR selectivity was not determined
    • How K27-linked ubiquitination is distinguished from K48-linked degradative ubiquitination in cells was unclear
  11. 2016 High

    Identifying transportin-1 as the nuclear import receptor for YBX1, with NLS point mutations (P201A/Y202A) abolishing import, resolved the long-debated mechanism of YBX1 nuclear entry.

    Evidence Co-IP of YBX1/transportin-1, in vitro nuclear import assay with M9M inhibitor, NLS mutagenesis in HeLa cells

    PMID:27794479

    Open questions at the time
    • Whether transportin-1-mediated import is the sole pathway or functions alongside proteolytic cleavage was not resolved
  12. 2017 High

    Three studies in 2017 expanded understanding of YBX1 sorting and signaling: YBX1 recognizes specific RNA hairpin motifs in exosomal mRNAs, is required for sorting miRNAs/tRNAs/Y RNAs into exosomes, and its phosphorylation at Ser-176 regulates NF-κB activation.

    Evidence RNA hairpin pull-down/MS (PMID:28341602); TGIRT-seq of CD63+ exosomes from YBX1-depleted cells (PMID:29073095); S176A mutagenesis with NF-κB reporter assays (PMID:28077578)

    PMID:28077578 PMID:28341602 PMID:29073095

    Open questions at the time
    • Whether YBX1 directly packages RNA into ILVs or acts upstream was unknown
    • Full complement of NF-κB target genes regulated via S176 was not defined
  13. 2019 High

    Demonstrating that acetylation at K81 blocks YBX1 binding to 5′UTRs of NFE2L2, HIF1A, and G3BP1—with K81A acetylation-resistant mutant restoring metastasis—identified a druggable post-translational switch controlling oncogenic translation.

    Evidence Global acetylomics, K81A mutagenesis, 5′UTR binding assays, in vivo metastasis rescue with HDAC inhibitor MS-275

    PMID:31668005

    Open questions at the time
    • Which acetyltransferase targets K81 was not identified
    • Whether K81 acetylation affects splicing or mRNA stability functions was untested
  14. 2020 High

    A cluster of 2020 findings unified the phosphorylation–localization–function axis: cell-cycle-regulated dephosphorylation at S102/S165/S176 exposes the NLS for G2/M nuclear entry; cytoplasmic phospho-YBX1 stabilizes Col1a1 mRNA to promote fibrosis while nuclear YBX1 represses the Col1a1 promoter; JAK2 phosphorylates YBX1 in myeloproliferative neoplasms to control splicing fidelity; and the CSD crystal structure showed S102 phosphorylation destabilizes the domain.

    Evidence Live-cell imaging, MS, and MD simulations of cell-cycle localization (PMID:32013098); Yb1+/− UUO model and HSc025 nuclear shuttling (PMID:27591085); phosphoproteomics in JAK2V617F cells with splicing analysis (PMID:33239784); crystal structure of CSDex with NMR and phosphomimetics (PMID:32710623)

    PMID:27591085 PMID:32013098 PMID:32710623 PMID:33239784

    Open questions at the time
    • Which phosphatase(s) mediate G2/M dephosphorylation in vivo was not identified beyond calcineurin at S102
    • Whether the fibrotic compartment switch operates in organs beyond kidney was not tested
  15. 2021 High

    Two 2021 discoveries provided biophysical mechanisms: YBX1 undergoes liquid–liquid phase separation to selectively recruit miRNAs for exosomal sorting, and YBX1 unwinds RNA secondary structures ATP-independently via its CSD, facilitating stress granule disassembly after stress.

    Evidence In vitro LLPS assays with LLPS-deficient mutants and miR-223 sorting (PMID:34766549); in vitro RNA unwinding with CSD mutagenesis and live-cell SG imaging in KO cells (PMID:34469566)

    PMID:34469566 PMID:34766549

    Open questions at the time
    • Whether LLPS and RNA unwinding are mechanistically coupled was unknown
    • In vivo confirmation of LLPS in endogenous exosome biogenesis was lacking
  16. 2022 High

    YBX1 was shown to form a phosphorylation-dependent transcriptional complex with KLF5 and to stabilize KLF5 mRNA in an m5C-dependent manner, while circIPO7 was found to scaffold AKT-mediated YBX1 phosphorylation and nuclear translocation, revealing circRNA-mediated and epitranscriptomic layers of YBX1 regulation.

    Evidence Co-IP, ChIP-seq, RSK2 kinase assays, m5C modification assays for YBX1/KLF5 (PMID:35022570); RNA pulldown, AKT Co-IP, nuclear fractionation for circIPO7 (PMID:35917517)

    PMID:35022570 PMID:35917517

    Open questions at the time
    • How many mRNAs are regulated by YBX1 in an m5C-dependent manner was not determined genome-wide
    • Whether circRNA scaffolding of YBX1 phosphorylation is a general mechanism was unknown
  17. 2025 High

    Demonstrating that YBX1 binds m5C sites in the RNF115 3′UTR and bridges to EIF4A1 at the 5′UTR to promote mRNA circularization and translation established a closed-loop translational activation mechanism for YBX1 with implications for ferroptosis regulation.

    Evidence RIP-seq, ribosome sequencing, bisulfite sequencing, Co-IP–MS, MeRIP-qPCR, dual-luciferase reporters, and xenograft models

    PMID:40088428

    Open questions at the time
    • Generality of YBX1-mediated mRNA circularization beyond RNF115 is unknown
    • Whether EIF4A1 interaction is m5C-dependent or constitutive was not resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis for 5′UTR selectivity among oncogenic targets, the identity of the acetyltransferase targeting K81, how cells coordinate opposing MMR activities in nucleus versus mitochondria, and whether LLPS-mediated RNA sorting operates broadly in exosome biogenesis in vivo.
  • No acetyltransferase for K81 identified
  • Structural basis of 5′UTR selectivity not resolved
  • In vivo LLPS contribution to exosome biogenesis not confirmed
  • Integration of nuclear MMR inhibition with mitochondrial MMR support unclear

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 9 GO:0140110 transcription regulator activity 5 GO:0003677 DNA binding 3 GO:0008092 cytoskeletal protein binding 3 GO:0098772 molecular function regulator activity 3 GO:0044183 protein folding chaperone 1
Localization
GO:0005829 cytosol 8 GO:0005634 nucleus 7 GO:0005576 extracellular region 3 GO:0005815 microtubule organizing center 2 GO:0005739 mitochondrion 1
Pathway
R-HSA-8953854 Metabolism of RNA 7 R-HSA-392499 Metabolism of proteins 6 R-HSA-162582 Signal Transduction 4 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-1640170 Cell Cycle 3 R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-73894 DNA Repair 3
Partners
EIF4A1HACE1KLF5PCNASMAD2TNPO1TSG101U2AF2
Complex memberships
MutSα/PCNA complex (inhibitory interaction)YBX1 mRNP complex

Evidence

Reading pass · 49 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 YB-1 (p50) is a potent cap-dependent mRNA stabilizer; its cold shock domain mediates mRNA stabilization by competing with eIF4E for binding at or near the mRNA 5' cap, and YB-1 depletion accelerates mRNA decay in vitro and in vivo. In vitro mRNA stability assays, eIF4E cap-binding competition assays, YB-1 overexpression and depletion experiments The EMBO journal High 11574481
2001 YB-1/p50 promotes rapid annealing of complementary RNA and DNA strands (up to 1500-fold acceleration) and can catalyze strand exchange; at low concentrations it promotes duplex formation, while excess YB-1 unwinds double-stranded forms. Phosphorylation selectively inhibits DNA annealing. In vitro nucleic acid annealing and strand-exchange assays across salt/temperature ranges; phosphorylation experiments The Journal of biological chemistry High 11585833
2004 At low YB-1/mRNA ratios (polysomal), YB-1 binds mRNA as a monomer using both RNA-binding domains; at high ratios (untranslatable mRNPs), YB-1 forms multimeric complexes (~700 kDa, ~20 subunits) that pack ~600–700 nt of mRNA on their surface, correlating with translational repression. Atomic force microscopy, electron microscopy, biochemical sedimentation, in vitro complex reconstitution at defined YB-1:mRNA ratios Nucleic acids research High 15494450
2006 Akt phosphorylates YB-1, and phospho-YB-1 fails to inhibit cap-dependent translation (but not IRES-dependent translation) of reporter mRNAs in vitro; Akt co-associates with inactive mRNPs and can relieve translational repression of YB-1-bound mRNAs. In vitro translation assays, co-immunoprecipitation of Akt with mRNPs, Affymetrix microarray identification of YB-1-associated mRNAs, phosphorylation-deficient mutant analysis Molecular and cellular biology High 16354698
2005 YB-1 autoregulates its own translation by binding a specific element (UCCAG/ACAA motif) in the 3'UTR of its own mRNA, causing accumulation of free mRNPs and inhibiting translation at the initiation step; PABP competes with YB-1 for this element and restores translational activity. In vitro translation assays, RNA binding mapping (UCCAG/ACAA for YB-1, ~50-nt A-rich for PABP), mRNP sedimentation analysis Molecular and cellular biology High 15798215
1995 YB-1 binds single-stranded DNA with higher affinity than double-stranded DNA at the MHC class II DRA promoter; it binds ssDNA at the X box and dsDNA at the Y box, and induces or stabilizes single-stranded regions in the promoter (detected by mung bean nuclease), consistent with a repression model that prevents loading of transactivating factors. EMSA with purified recombinant YB-1, methylation interference mapping, mung bean nuclease analysis, YB-1-specific antibodies on endogenous protein The Journal of biological chemistry High 7876087
2005 YB-1 knockout mouse embryos show late embryonic lethality (after E13.5); YB-1−/− fibroblasts exhibit reduced stress responses, premature senescence under oxidative stress, and elevated CDK inhibitors p16Ink4a and p21Cip1; the senescence defect is rescued by siRNA knockdown of CDK inhibitors, indicating YB-1 normally represses CDK inhibitor transcription. Knockout mouse model, siRNA rescue experiments, stress assays, transcriptome/proteome profiling Molecular and cellular biology High 15899865
2007 PP2Cgamma phosphatase interacts with YB-1 via its acidic domain to regulate alternative splicing of CD44 exons v4 and v5; PP2Cgamma phosphorylation enhances its interaction with YB-1, and PP2Cgamma knockdown affects CD44 alternative splicing in a YB-1-dependent manner. Co-IP, phosphatase activity assays in vitro splicing, siRNA knockdown, in vivo splicing assays Nature structural & molecular biology High 17572683
2012 YB-1 binds CAUC and CACC motifs (defined by SELEX), which function as splicing enhancers; YB-1 activates exon inclusion at weak 3' splice sites by facilitating recruitment of U2AF65 to weak polypyrimidine tracts through direct protein–protein interaction with U2AF65. SELEX, in vivo splicing minigene assays, direct protein–protein interaction assays between YB-1 and U2AF65, endogenous CD44 exon v5 analysis Nucleic acids research High 22730292
2008 YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro; electron and atomic force microscopy show that YB-1 coats the outer microtubule wall; YB-1 also promotes assembly of MAPs-tubulin and subtilisin-treated tubulin, and tubulin competes with RNA for YB-1 binding. In vitro microtubule assembly assays, electron microscopy, atomic force microscopy, direct binding assays BMC biochemistry High 18793384
2009 YB-1 localizes to human mitochondria and contributes to mitochondrial mismatch repair (MMR) activity; YB-1 depletion significantly reduces mitochondrial mismatch-binding and MMR activity in HeLa cell extracts and increases mitochondrial DNA mutagenesis. Mitochondrial fractionation, mismatch-binding assays with mitochondrial extracts, siRNA depletion, mitochondrial DNA mutagenesis assay DNA repair High 19272840
2015 Stress-induced tRNA-derived fragments (tRFs) from tRNA(Glu), tRNA(Asp), tRNA(Gly), and tRNA(Tyr) suppress oncogenic transcript stability by displacing 3'UTRs from YBX1 in a sequence-specific manner; the tRFs share a motif matching the YBX1 recognition sequence, and this displacement-based silencing suppresses breast cancer invasion and metastasis. RNA immunoprecipitation, LNA anti-sense inhibition, synthetic RNA mimetics, gain-/loss-of-function assays, in vivo metastasis models Cell High 25957686
2015 YB-1 directly binds to and translationally activates the 5'UTR of HIF1A mRNA, increasing HIF1α protein expression in sarcoma cells, which drives invasion and metastasis via a YB-1–HIF1α axis. Direct RNA binding assays, translational reporter assays, loss-of-function in vitro and in vivo metastasis models Cancer cell High 25965573
2015 YB-1 directly binds the 5'UTR of G3BP1 mRNA and translationally activates it, controlling G3BP1 availability for stress granule nucleation; YB-1 inactivation dramatically reduces G3BP1 protein levels and stress granule formation in sarcoma cells. Direct RNA binding (5'UTR), translational reporter assays, YB-1 inactivation with defined G3BP1/SG phenotype, in vivo xenograft models The Journal of cell biology High 25800057
2015 HACE1 E3 ubiquitin ligase polyubiquitinates YB-1 through non-canonical K27-linked ubiquitin chains; these linkages are required for YB-1 interaction with TSG101 (MVB pathway component) and for its extracellular secretion. Secreted YB-1 inhibits TGF-β-mediated EMT. E3 ligase identification, ubiquitin chain-type analysis, Co-IP of YB-1 with TSG101, secretion assays, TGF-β functional assay Cellular signalling High 26343856
2019 Class I HDAC inhibitor MS-275 promotes acetylation of YB-1 at lysine-81, blocking YB-1 binding to 5'UTRs of NFE2L2 (NRF2), HIF1A, and G3BP1 mRNAs and reducing their translation; a YB-1(K81A) mutant resistant to acetylation restores metastatic capacity and target protein synthesis. Global acetylomics, site-directed mutagenesis (K81A), 5'UTR binding assays, translational assays, in vivo metastasis rescue EMBO reports High 31668005
2020 YBX1 is a target of JAK2 phosphorylation; inactivation of YBX1 in JAK2V617F cells causes RNA mis-splicing (intron retention), disrupts transcriptional control of ERK signaling, and sensitizes persisting cells to apoptosis; combined JAK inhibition + YBX1 inactivation induces molecular remission in mouse models. In-depth phosphoproteome profiling, genetic inactivation, RNA splicing analysis, in vivo JAK2-mutated mouse models, primary human cells Nature High 33239784
2020 The structural basis of YB-1 CSD DNA binding was determined; the C-terminal extension of the CSD adopts a rigid structure interacting with core CSD residues and provides a unique DNA binding site. Phosphorylation of S102 destabilizes the CSD, causes partial unfolding, and dramatically reduces ssDNA binding affinity. Crystal structure of YB-1 CSDex, NMR, ssDNA binding assays, phosphomimetic and deletion mutants Nucleic acids research High 32710623
2021 YBX1 undergoes liquid-liquid phase separation (LLPS) in vitro and in cells; YBX1 condensates selectively recruit miR-223 in vitro and promote its sorting into exosomes. Point mutations that inhibit LLPS impair YBX1 incorporation into condensates and perturb miR-223 sorting into exosomes. In vitro LLPS assays, live-cell imaging of condensates, miRNA sorting assays, LLPS-deficient point mutants, exosome isolation eLife High 34766549
2021 YBX1 promotes translation of oncogenic transcripts including MYC by recruitment to polysomal chains; genetic inactivation of YBX1 in AML cells displaces MYC and other oncogenic drivers from polysomes, depleting their protein levels and reducing leukemia cell fitness. Ribosome/polysome profiling, genetic inactivation (KO), protein level analysis, in vitro and in vivo competition assays Leukemia High 34465866
2013 YB-1 disrupts MutSα (MSH2-MSH6) MMR complex formation by interacting with PCNA via two PIP-box motifs in its C-terminal domain; this interaction reduces MutSα mismatch-binding activity, disrupts MutSα/PCNA/heteroduplex ternary complex formation, and inhibits in vitro MMR activity. YB-1 overexpression is associated with microsatellite instability and increased mutation rates. In vitro MMR assays, PIP-box mutagenesis, Co-IP, PCNA interaction mapping, microsatellite instability assays, mutation frequency measurements Oncogene High 24141788
2016 Transportin-1 mediates YB-1 nuclear import; YB-1/transportin-1 complex is isolated from HeLa extracts; nuclear import is abolished by a competitor substrate or transportin-1 inhibitor M9M but not by importin-β1 inhibitors; inactivating mutations in a transportin-1-dependent NLS (P201A/Y202A) abolish transport. Co-immunoprecipitation from cell extracts, in vitro nuclear import assay, competitor/inhibitor experiments, NLS point mutagenesis Biochemical and biophysical research communications High 27794479
2020 YB-1 nuclear localization is cell-cycle regulated: YB-1 is perinuclear during G1/S and enters the nucleus during late G2/M, exiting at mitosis completion. Dephosphorylation at S102, S165, and S176 increases accessibility of the NLS (shown by MD simulations), facilitating nuclear entry; no proteolytic cleavage is required. Live-cell confocal imaging, mass spectrometry of phosphorylation sites, atomistic MD simulations, doxorubicin-induced G2 arrest Cancers High 32013098
2020 Phospho-YB-1 (S102) cytoplasmic YB-1 directly stabilizes Col1a1 mRNA, promoting fibrosis; forced nuclear compartmentalization of phosphorylated YB-1 by small molecule HSc025 instead represses the Col1a1 promoter and attenuates fibrosis in vivo. Yb1+/- mouse model of unilateral ureteral obstruction, phospho-specific antibodies, mRNA stability assays, pharmacological nuclear shuttling with HSc025 Kidney international High 27591085
2020 CircRNA-SORE binds YBX1 in the cytoplasm, preventing its interaction with the E3 ubiquitin ligase PRP19 and thus blocking PRP19-mediated YBX1 ubiquitination and degradation, thereby stabilizing YBX1 protein. RNA pulldown, Co-IP, PRP19 ubiquitination assays, circRNA knockdown in vitro and in vivo Signal transduction and targeted therapy High 33361760
2018 Ionizing radiation and EGF induce RSK (p90 ribosomal S6 kinase) nuclear translocation, where RSK phosphorylates already-nuclear YB-1 at Ser-102; acute stimulation does not cause cytoplasm-to-nucleus translocation of YB-1 itself. In KRAS-mutant cells, YB-1 is constitutively phosphorylated. Nuclear/cytoplasmic fractionation, phospho-specific antibodies, pharmacological RSK/EGFR inhibitors, conditional KRAS(G12V) expression International journal of molecular sciences High 30126195
2019 Inhibition of RNA polymerase II triggers phosphorylation of YB-1 at Ser102 and its nuclear accumulation; kinase inhibition reduces both phosphorylation and nuclear accumulation; nuclear RNA presence is required for nuclear retention of YB-1. RNAPII inhibitors, kinase inhibitors, nuclear fractionation, immunofluorescence, RNA depletion experiments Cells High 31906126
2015 Poly(ADP-ribose)polymerase 1 (PARP1) poly(ADP-ribosyl)ates YB-1 in the presence of damaged DNA in vitro, identifying PARylation as a novel post-translational modification of YB-1 linked to the DNA damage response. In vitro PARylation assay with PARP1, damaged DNA substrate, Western blot for PAR-modified YB-1 Biochimie Medium 26453809
2014 Calcineurin (CN) dephosphorylates YB-1 at Ser-102, preventing phospho-YB-1 binding to and transactivation of the CCL5 promoter during monocyte differentiation. Phospho-YB-1(Ser-102), generated via Akt, shows stronger CCL5 promoter binding; CN directly interacts with YB-1 (demonstrated by Co-IP). Co-IP of YB-1/calcineurin, phosphatase assay, ChIP on CCL5 promoter, pharmacological calcineurin inhibition (cyclosporine A) in vivo The Journal of biological chemistry High 24947514
2017 YB-1 interacts directly with Smad2 and stabilizes it by attenuating Smad2 ubiquitination; Smad2 acts as a transcription factor that activates the YB-1 promoter, forming a positive feedback loop. Ser102 phosphorylation of YB-1 is required for both its binding to and stabilization of Smad2. Co-IP, ubiquitination assays, promoter reporter assays, YB-1 Ser102 mutagenesis, in vivo CCl4 liver fibrosis model Biochemical and biophysical research communications Medium 28153731
2017 YB-1 and cytosolic NSUN2 are the only proteins in HEK293 cytosolic extract that specifically recognize three structural RNA motifs (ACCAGCCU, CAGUGAGC, UAAUCCCA) enriched in exosomal mRNAs, as shown by pull-down with RNA hairpins; both proteins are found in exosomes. RNA hairpin pull-down from cytosolic extract, mass spectrometry identification, exosome isolation and protein detection Biochimica et biophysica acta. Proteins and proteomics Medium 28341602
2017 YBX1 is required for sorting of multiple small ncRNA species (miRNAs, tRNAs, Y RNAs, Vault RNAs) into exosomes, as demonstrated by TGIRT-seq of immunoisolated CD63+ exosomes from YBX1-depleted HEK293T cells. TGIRT-seq, flotation gradient ultracentrifugation, CD63 immunoisolation of exosomes, YBX1 knockdown Proceedings of the National Academy of Sciences of the United States of America High 29073095
2002 In human myxoid liposarcoma cells, wild-type TLS binds to YB-1 through its C-terminal domain and cooperates with YB-1 to promote RNA splicing (13S isoform of adenovirus E1A pre-mRNA). The oncogenic TLS/CHOP fusion protein lacks the ability to recruit YB-1 and inhibits YB-1-mediated splicing in a dominant-negative manner. Co-IP of TLS and YB-1, in vivo splicing assay with adenovirus E1A minigene, TLS/CHOP dominant-negative analysis Journal of orthopaedic research Medium 12168660
2001 YB-1 (dbpB) is proteolytically cleaved from mRNA upon thrombin stimulation of endothelial cells, releasing an active ~30 kDa species that translocates to the nucleus and activates thrombin-responsive gene transcription; cytosolic full-length dbpB is mRNA-bound, and RNase treatment of cytosolic extracts activates it. Oligo(dT) affinity chromatography, Nycodenz gradient fractionation, RNase treatment, reporter transfection assays, immunoprecipitation Proceedings of the National Academy of Sciences of the United States of America High 11390977
2000 YB-1 co-immunoprecipitates with FMRP (fragile X mental retardation protein) and is identified as a component of the FMRP-associated mRNP complex by mass spectrometry. Co-immunoprecipitation from Flag-Fmr1 stable cell lines, mass spectrometry identification, anti-p50 antibody confirmation Biochemical and biophysical research communications Medium 11162447
2008 MBNL1 forms an RNP complex with YB-1 and DDX1 (demonstrated by GST pulldown); under stress, MBNL1 co-localizes with YB-1 at cytoplasmic stress granules. GST pulldown, co-immunoprecipitation, stress granule co-localization by immunofluorescence Journal of neuroscience research Medium 18335541
2005 Nuclear YB-1 selectively inhibits p53's ability to cause cell death and to transactivate cell death genes, but does not interfere with p53-mediated transactivation of CDKN1A (p21) or MDM2; transcriptionally active p53 is required for nuclear localization of YB-1. Co-expression experiments, transcriptional reporter assays for p53 target genes, cell death assays, mammary epithelial cell p53 stress activation Oncogene Medium 16158057
2011 YB-1 localizes to the centrosome in a phosphorylation-dependent manner, where it complexes with pericentrin and γ-tubulin, maintaining structural integrity and microtubule nucleation capacity; YB-1 overexpression in mammary epithelial cells causes cytokinesis failure and centrosome amplification. Antibody microarray, co-immunoprecipitation of YB-1 with pericentrin and γ-tubulin, centrosome localization imaging, conditional YB-1 expression in HMECs Oncogene Medium 21423216
2020 YB-1 depletion causes cytokinesis failure and multinucleation; phosphorylation at six serine residues (including novel sites identified by mass spectrometry) is required for cytokinesis; YB-1 orchestrates spatio-temporal distribution of microtubules, β-actin, and the chromosome passenger complex (CPC) to define the cleavage plane. siRNA depletion with rescue, confocal imaging of cytokinesis structures, mass spectrometry for phosphorylation sites, atomistic MD modelling Cancers High 32882852
2015 Phosphorylated YB-1 accumulates in the centrosome at mitotic phase; YB-1 knockdown causes microtubule detachment from the centrosome at telophase and nuclear lobulation due to defective nuclear envelope reassembly from mis-localized non-centrosomal microtubules. YB-1 knockdown, immunofluorescence of centrosomal YB-1, microtubule localization analysis, nuclear morphology assays Scientific reports Medium 25740062
2021 YB-1 unwinds mRNA secondary structures without ATP consumption via its conserved CSD (which destabilizes RNA stem/loops) and its unstructured C-terminal domain (which secures unwinding); at endogenous levels YB-1 facilitates stress granule disassembly after stress recovery; overexpression inhibits stress granule assembly. In vitro RNA unwinding assays, CSD mutagenesis, live-cell imaging of stress granules in YB-1 KO and overexpressing cells, arsenite stress Nucleic acids research High 34469566
2004 YB-1 physically associates with Sox21 and partially restores NGF-induced neurite outgrowth inhibited by Sox21 overexpression in PC12 cells, indicating YB-1 can antagonize Sox21-mediated repression of neuronal differentiation. Co-immunoprecipitation of endogenous proteins, neurite outgrowth rescue assay Neuroscience letters Low 15039105
2017 YBX1 phosphorylation at Ser-176, identified by mass spectrometry, is required for NF-κB activation; YBX1-S176A mutant shows dramatically reduced NF-κB-activating ability compared to WT, and Ser-176 and Ser-165 regulate distinct subsets of NF-κB target genes. Mass spectrometry identification of S176 phosphosite, S176A mutagenesis, NF-κB reporter assays The Journal of biological chemistry Medium 28077578
2022 RSK2-mediated phosphorylation of YB-1 at Ser102 promotes formation of a YB-1/KLF5 transcriptional complex; this complex co-regulates expression of KRT16 and Ly6D to promote basal-like breast cancer proliferation. YB-1 also stabilizes KLF5 mRNA in an RNA 5-methylcytosine modification-dependent manner. Co-IP of YB-1/KLF5, RSK2 kinase assays, m5C modification assays, ChIP-seq, luciferase reporters, RSK inhibitor LJH685 in vivo Cell death and differentiation High 35022570
2022 CircIPO7 binds YBX1 protein in the cytoplasm, facilitates its phosphorylation at Ser102 by AKT, and promotes YBX1 nuclear translocation, leading to transcriptional activation of FGFR1, TNC, and NTRK1. RNA-protein pulldown, Co-IP, nuclear fractionation, phosphorylation assays, luciferase reporter assays, in vitro and in vivo metastasis assays Clinical cancer research Medium 35917517
2012 YB-1 mRNA translation is regulated by the mTOR signaling pathway; specific mTOR inhibition with PP242 (but not rapamycin) suppresses YB-1 synthesis; this dependence is dictated by the 5'UTR of YB-1 mRNA (shown by reporter constructs). Western/Northern blotting, mTOR inhibitor (PP242 vs. rapamycin) treatment, 5'UTR reporter constructs PloS one Medium 23285076
2025 YBX1 binds to m5C modification sites in the RNF115 mRNA 3'UTR and interacts with EIF4A1 to bridge the 5'UTR, promoting mRNA circularization and translation of RNF115; NSUN2 is responsible for m5C modification of RNF115 mRNA; YBX1-driven RNF115 translation suppresses ferroptosis via DHODH ubiquitination. RIP-seq, ribosome sequencing, bisulfite sequencing, Co-IP mass spectrometry, RNA pulldown, MeRIP-qPCR, dual-luciferase reporter, xenograft models Clinical and translational medicine High 40088428
2024 ZO-1 interacts with YB-1 in endothelial cells; arsenite-induced stress decreases ZO-1/YB-1 interaction and drives stress granule assembly; YB-1 expression is essential for stress granule formation and for cytoprotective effects induced by ZO-1 downregulation; endothelial-specific ZO-1 deletion increases YB-1-positive granules in retinal vessels and alters angiogenesis. Proteome-wide interactome, Co-IP, live-cell imaging, endothelial-specific conditional KO mice, retinal vascular imaging Nature communications High 38782923
2020 YBX1 controls thermogenic adipocyte differentiation (browning) in white adipose tissue; YBX1 knockdown impairs thermogenic potential of brown and brite preadipocytes; CRISPRa-induced YBX1 expression promotes browning; transcriptomic analysis shows YBX1 regulates the histone H3K9 demethylase Jmjd1c to promote thermogenic commitment. Temporal proteomics of cold-exposed scWAT, siRNA KD, CRISPRa induction, transcriptomic analysis, mitochondrial respiration assays Molecular metabolism Medium 33285300

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2015 Endogenous tRNA-Derived Fragments Suppress Breast Cancer Progression via YBX1 Displacement. Cell 723 25957686
2003 The pleiotropic functions of the Y-box-binding protein, YB-1. BioEssays : news and reviews in molecular, cellular and developmental biology 429 12815724
2020 CircRNA-SORE mediates sorafenib resistance in hepatocellular carcinoma by stabilizing YBX1. Signal transduction and targeted therapy 363 33361760
2013 YB-1 protein: functions and regulation. Wiley interdisciplinary reviews. RNA 316 24217978
2017 Broad role for YBX1 in defining the small noncoding RNA composition of exosomes. Proceedings of the National Academy of Sciences of the United States of America 272 29073095
2015 Translational Activation of HIF1α by YB-1 Promotes Sarcoma Metastasis. Cancer cell 244 25965573
2001 The major mRNA-associated protein YB-1 is a potent 5' cap-dependent mRNA stabilizer. The EMBO journal 238 11574481
2006 Akt-mediated YB-1 phosphorylation activates translation of silent mRNA species. Molecular and cellular biology 228 16354698
2015 YB-1 regulates stress granule formation and tumor progression by translationally activating G3BP1. The Journal of cell biology 221 25800057
2021 Circular RNA ACTN4 promotes intrahepatic cholangiocarcinoma progression by recruiting YBX1 to initiate FZD7 transcription. Journal of hepatology 181 34509526
2009 Novel DNA mismatch-repair activity involving YB-1 in human mitochondria. DNA repair 162 19272840
2004 YB-1 is upregulated during prostate cancer tumor progression and increases P-glycoprotein activity. The Prostate 149 15042610
2005 YB-1 is important for late-stage embryonic development, optimal cellular stress responses, and the prevention of premature senescence. Molecular and cellular biology 148 15899865
2021 Selective sorting of microRNAs into exosomes by phase-separated YBX1 condensates. eLife 130 34766549
1995 The transcriptional regulatory protein, YB-1, promotes single-stranded regions in the DRA promoter. The Journal of biological chemistry 128 7876087
2020 Splicing factor YBX1 mediates persistence of JAK2-mutated neoplasms. Nature 127 33239784
2009 Reduced proliferation and enhanced migration: two sides of the same coin? Molecular mechanisms of metastatic progression by YB-1. Cell cycle (Georgetown, Tex.) 120 19713745
2005 YB-1 provokes breast cancer through the induction of chromosomal instability that emerges from mitotic failure and centrosome amplification. Cancer research 120 15899797
2004 Structural organization of mRNA complexes with major core mRNP protein YB-1. Nucleic acids research 117 15494450
2019 Class I HDAC inhibitors enhance YB-1 acetylation and oxidative stress to block sarcoma metastasis. EMBO reports 94 31668005
2012 YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts. Nucleic acids research 90 22730292
2017 Cytosolic YB-1 and NSUN2 are the only proteins recognizing specific motifs present in mRNAs enriched in exosomes. Biochimica et biophysica acta. Proteins and proteomics 87 28341602
2021 A comprehensive review of the functions of YB-1 in cancer stemness, metastasis and drug resistance. Cellular signalling 85 34224843
2005 Y-box factor YB1 controls p53 apoptotic function. Oncogene 84 16158057
2001 The major messenger ribonucleoprotein particle protein p50 (YB-1) promotes nucleic acid strand annealing. The Journal of biological chemistry 77 11585833
2018 YBX1 at the crossroads of non-coding transcriptome, exosomal, and cytoplasmic granular signaling. European journal of cell biology 75 29478751
2005 YB-1 autoregulates translation of its own mRNA at or prior to the step of 40S ribosomal subunit joining. Molecular and cellular biology 75 15798215
2000 Identification of mouse YB1/p50 as a component of the FMRP-associated mRNP particle. Biochemical and biophysical research communications 69 11162447
2006 Cold shock domain family members YB-1 and MSY4 share essential functions during murine embryogenesis. Molecular and cellular biology 68 16954378
2022 YB-1 is a positive regulator of KLF5 transcription factor in basal-like breast cancer. Cell death and differentiation 65 35022570
2008 MBNL1 associates with YB-1 in cytoplasmic stress granules. Journal of neuroscience research 62 18335541
2022 CircIPO7 Promotes Nasopharyngeal Carcinoma Metastasis and Cisplatin Chemoresistance by Facilitating YBX1 Nuclear Localization. Clinical cancer research : an official journal of the American Association for Cancer Research 60 35917517
2007 Alternative splicing regulation by interaction of phosphatase PP2Cgamma with nucleic acid-binding protein YB-1. Nature structural & molecular biology 60 17572683
2017 Novel Serine 176 Phosphorylation of YBX1 Activates NF-κB in Colon Cancer. The Journal of biological chemistry 59 28077578
2015 HACE1 mediated K27 ubiquitin linkage leads to YB-1 protein secretion. Cellular signalling 54 26343856
2019 Requirement of the transcription factor YB-1 for maintaining the stemness of cancer stem cells and reverting differentiated cancer cells into cancer stem cells. Stem cell research & therapy 52 31375149
2011 Impact of oncogenic K-RAS on YB-1 phosphorylation induced by ionizing radiation. Breast cancer research : BCR 51 21392397
2020 LINC00857 Interacting with YBX1 to Regulate Apoptosis and Autophagy via MET and Phosphor-AMPKa Signaling. Molecular therapy. Nucleic acids 48 33312753
2014 YB-1 promotes transcription of cyclin D1 in human non-small-cell lung cancers. Genes to cells : devoted to molecular & cellular mechanisms 47 24774443
2001 Thrombin induces the release of the Y-box protein dbpB from mRNA: a mechanism of transcriptional activation. Proceedings of the National Academy of Sciences of the United States of America 47 11390977
2022 YB-1 as an Oncoprotein: Functions, Regulation, Post-Translational Modifications, and Targeted Therapy. Cells 46 35406781
2011 YB-1 evokes susceptibility to cancer through cytokinesis failure, mitotic dysfunction and HER2 amplification. Oncogene 46 21423216
2020 YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells. BMC cancer 45 33054752
2020 Soyasaponin II protects against acute liver failure through diminishing YB-1 phosphorylation and Nlrp3-inflammasome priming in mice. Theranostics 44 32194830
2021 YBX1 mediates translation of oncogenic transcripts to control cell competition in AML. Leukemia 41 34465866
1999 YB-1 regulation of the human and rat gelatinase A genes via similar enhancer elements. Journal of the American Society of Nephrology : JASN 41 10589685
2023 Circular RNA cFAM210A, degradable by HBx, inhibits HCC tumorigenesis by suppressing YBX1 transactivation. Experimental & molecular medicine 39 37907737
2020 YB-3 substitutes YB-1 in global mRNA binding. RNA biology 39 31944153
2020 Structural basis of DNA binding to human YB-1 cold shock domain regulated by phosphorylation. Nucleic acids research 39 32710623
2013 Links between the oncoprotein YB-1 and small non-coding RNAs in breast cancer. PloS one 39 24260353
2008 YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules. BMC biochemistry 38 18793384
2024 PIWI-interacting RNA-YBX1 inhibits proliferation and metastasis by the MAPK signaling pathway via YBX1 in triple-negative breast cancer. Cell death discovery 36 38182573
2021 LncRNA BASP1-AS1 interacts with YBX1 to regulate Notch transcription and drives the malignancy of melanoma. Cancer science 35 34533860
2014 Calcineurin-mediated YB-1 dephosphorylation regulates CCL5 expression during monocyte differentiation. The Journal of biological chemistry 35 24947514
2019 YB-1 modulates the drug resistance of glioma cells by activation of MDM2/p53 pathway. Drug design, development and therapy 34 30679904
2016 Therapeutic nuclear shuttling of YB-1 reduces renal damage and fibrosis. Kidney international 34 27591085
2019 Inhibition of Transcription Induces Phosphorylation of YB-1 at Ser102 and Its Accumulation in the Nucleus. Cells 33 31906126
2016 YBX1 regulates tumor growth via CDC25a pathway in human lung adenocarcinoma. Oncotarget 33 27384875
2017 YB-1 regulates tumor growth by promoting MACC1/c-Met pathway in human lung adenocarcinoma. Oncotarget 32 28624808
2014 HnRNP C, YB-1 and hnRNP L coordinately enhance skipping of human MUSK exon 10 to generate a Wnt-insensitive MuSK isoform. Scientific reports 32 25354590
2022 CBX3 Regulated By YBX1 Promotes Smoking-induced Pancreatic Cancer Progression via Inhibiting SMURF2 Expression. International journal of biological sciences 31 35637952
2018 Oxidative Stress Causes Enhanced Secretion of YB-1 Protein that Restrains Proliferation of Receiving Cells. Genes 31 30360431
2012 YB-1 synthesis is regulated by mTOR signaling pathway. PloS one 30 23285076
2020 YB-1 interplays with ERα to regulate the stemness and differentiation of ER-positive breast cancer stem cells. Theranostics 28 32206124
2015 Interplay between YB-1 and IL-6 promotes the metastatic phenotype in breast cancer cells. Oncotarget 28 26512918
2004 Sox21 is a repressor of neuronal differentiation and is antagonized by YB-1. Neuroscience letters 28 15039105
2002 RNA splicing mediated by YB-1 is inhibited by TLS/CHOP in human myxoid liposarcoma cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society 27 12168660
2021 YB-1 unwinds mRNA secondary structures in vitro and negatively regulates stress granule assembly in HeLa cells. Nucleic acids research 26 34469566
2020 YB-1: The key to personalised prostate cancer management? Cancer letters 26 32681926
2018 Stress-Induced Phosphorylation of Nuclear YB-1 Depends on Nuclear Trafficking of p90 Ribosomal S6 Kinase. International journal of molecular sciences 26 30126195
2016 Kindlin-2 interacts with β-catenin and YB-1 to enhance EGFR transcription during glioma progression. Oncotarget 26 27713156
2014 YB-1 regulates Sox2 to coordinately sustain stemness and tumorigenic properties in a phenotypically distinct subset of breast cancer cells. BMC cancer 26 24885403
2021 YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma. Frontiers in oncology 25 34976785
2023 YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells. Nature communications 24 36949044
2021 hTERT Promotes CRC Proliferation and Migration by Recruiting YBX1 to Increase NRF2 Expression. Frontiers in cell and developmental biology 23 34079797
2021 Novel Insights into YB-1 Signaling and Cell Death Decisions. Cancers 23 34282755
2020 Dephosphorylation of YB-1 is Required for Nuclear Localisation During G2 Phase of the Cell Cycle. Cancers 23 32013098
2015 Poly(ADP-ribosyl)ation as a new posttranslational modification of YB-1. Biochimie 23 26453809
2014 Differential control of interleukin-6 mRNA levels by cellular distribution of YB-1. PloS one 23 25398005
2013 YB-1 disrupts mismatch repair complex formation, interferes with MutSα recruitment on mismatch and inhibits mismatch repair through interacting with PCNA. Oncogene 22 24141788
2013 YB1 binds to and represses the p16 tumor suppressor gene. Genes to cells : devoted to molecular & cellular mechanisms 22 24165022
2020 White adipose remodeling during browning in mice involves YBX1 to drive thermogenic commitment. Molecular metabolism 21 33285300
2016 Transportin-1-dependent YB-1 nuclear import. Biochemical and biophysical research communications 21 27794479
2015 Noncoding RNAs that associate with YB-1 alter proliferation in prostate cancer cells. RNA (New York, N.Y.) 21 25904138
2024 ZO-1 interacts with YB-1 in endothelial cells to regulate stress granule formation during angiogenesis. Nature communications 20 38782923
2023 Long non-coding RNA KCND1 protects hearts from hypertrophy by targeting YBX1. Cell death & disease 20 37253771
2021 YBX1 knockdown induces renal cell carcinoma cell apoptosis via Kindlin-2. Cell cycle (Georgetown, Tex.) 20 34709966
2019 Chromobox homolog 8 (CBX8) Interacts with Y-Box binding protein 1 (YBX1) to promote cellular proliferation in hepatocellular carcinoma cells. Aging 20 31495785
2016 Nanog RNA-binding proteins YBX1 and ILF3 affect pluripotency of embryonic stem cells. Cell biology international 20 26289635
2025 Targeting YBX1-m5C mediates RNF115 mRNA circularisation and translation to enhance vulnerability of ferroptosis in hepatocellular carcinoma. Clinical and translational medicine 19 40088428
2020 YB-1 Interferes with TNFα-TNFR Binding and Modulates Progranulin-Mediated Inhibition of TNFα Signaling. International journal of molecular sciences 19 32992926
2015 Twist contributes to proliferation and epithelial-to-mesenchymal transition-induced fibrosis by regulating YB-1 in human peritoneal mesothelial cells. The American journal of pathology 19 26055210
2018 The Role of YB1 in Renal Cell Carcinoma Cell Adhesion. International journal of medical sciences 17 30275756
2024 Stabilization of KPNB1 by deubiquitinase USP7 promotes glioblastoma progression through the YBX1-NLGN3 axis. Journal of experimental & clinical cancer research : CR 16 38254206
2024 Microglia-derived exosomes selective sorted by YB-1 alleviate nerve damage and cognitive outcome in Alzheimer's disease. Journal of translational medicine 16 38755651
2023 Targeting YB-1 via entinostat enhances cisplatin sensitivity of pleural mesothelioma in vitro and in vivo. Cancer letters 16 37730104
2015 Centrosome maturation requires YB-1 to regulate dynamic instability of microtubules for nucleus reassembly. Scientific reports 16 25740062
2000 The selenocysteine insertion sequence binding protein SBP is different from the Y-box protein dbpB. Biochimie 16 10727766
2020 Critical Role for Cold Shock Protein YB-1 in Cytokinesis. Cancers 15 32882852
2017 Positive feedback loop of YB-1 interacting with Smad2 promotes liver fibrosis. Biochemical and biophysical research communications 15 28153731