Affinage

DDX6

Probable ATP-dependent RNA helicase DDX6 · UniProt P26196

Length
483 aa
Mass
54.4 kDa
Annotated
2026-06-09
98 papers in source corpus 45 papers cited in narrative 45 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

DDX6 (RCK/p54) is a conserved DEAD-box ATP-dependent RNA helicase that serves as a central scaffold for post-transcriptional gene regulation, coupling miRNA-mediated silencing, translational repression, mRNA decay, and cytoplasmic RNP granule assembly (PMID:24768538, PMID:24768540, PMID:31588046, PMID:38989862). It is recruited to miRNA-targeted transcripts through a direct interaction between its C-terminal RecA2 domain and the CNOT1 MIF4G domain, an arrangement structurally analogous to the eIF4A–eIF4G interaction that simultaneously stimulates DDX6 ATPase activity and links target recognition by the CCR4-NOT deadenylase to repression (PMID:24768538, PMID:24768540, PMID:25035296). DDX6 binds RNA without sequence specificity at high affinity, and ATP binding (not hydrolysis) drives relaxation of bound RNA; its concentration in cells far exceeds that of mRNA, consistent with coating transcripts within granules (PMID:22836354). The same shallow RecA2 groove engages the FDF motifs of the decapping activator EDC3 and translational repressor Tral/Pat1 in a mutually exclusive manner, while the 4E-T CUP-homology domain binds DDX6 alongside CNOT1 in a ternary complex, and DDX6's own RecA2 FDF motif tethers it to the ribosome to trigger deadenylation-dependent decay of inefficiently translated mRNAs (PMID:19285948, PMID:26489469, PMID:38989862). Translational repression additionally requires direct interaction with DDX3X to block 48S preinitiation complex assembly (PMID:40923767). Through these interactions DDX6 drives liquid-liquid phase separation into P-bodies that sequester repressed mRNPs, with 4E-T and LSM14A as obligate co-factors, and it concurrently limits aberrant stress granule formation in an ATPase- and RNA-binding–dependent manner (PMID:25995375, PMID:41330929, PMID:38536035). This activity governs cell-fate decisions: loss of DDX6 dissolves P-bodies and de-represses fate-instructive factors to lock embryonic stem cells in a hyper-pluripotent state, while in early embryogenesis its essential function operates through the miRNA pathway rather than P-body or decapping arms (PMID:31588046, PMID:36197846). DDX6 also restrains innate antiviral responses, limiting interferon-stimulated gene overactivation via the LSM1-dependent mRNA degradation machinery (PMID:28746868). De novo missense variants in the DDX6 RecA2 domain disrupt partner binding and P-body assembly, establishing this domain's integrity as required for human neurodevelopmental function (PMID:31422817).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2003 Medium

    Establishing that DDX6 is a bona fide ATP-dependent RNA helicase rather than merely an RNA-binding scaffold defined its core enzymatic capability.

    Evidence Surface plasmon resonance RNA binding and in vitro unwinding assays with recombinant rck/p54 on c-myc RNA, plus deletion mapping of the C-terminal domain

    PMID:12875652

    Open questions at the time
    • Physiological substrates beyond c-myc RNA not defined
    • No structural basis for the ATP dependence resolved here
  2. 2006 High

    A crystal structure of the N-terminal core resolved why DDX6 ATPase is uniquely regulated and linked the enzyme to cell-cycle control.

    Evidence X-ray crystallography of Nc-rck/p54 revealing an Asn131-induced closed P-loop, plus IRES helicase and HeLa cell-cycle assays

    PMID:16611246

    Open questions at the time
    • How partner binding relieves the closed conformation not shown
    • Link between c-myc downregulation and G2/M arrest correlative
  3. 2006 High

    Placing DDX6 physically within active RISC and showing it is required for miRNA repression but not siRNA cleavage separated the translational-repression arm of silencing from endonucleolytic cleavage.

    Evidence Affinity purification of active RISC, reciprocal Co-IP with Ago1/Ago2, and knockdown rescue across multiple miRNA targets in human cells

    PMID:16756390

    Open questions at the time
    • Molecular bridge connecting DDX6 to Ago not defined here
    • Whether interaction is direct or via adaptors unresolved
  4. 2009 High

    Mapping the FDF-motif binding groove on DDX6 explained how decapping activators and translational repressors compete for the helicase, defining a mutually exclusive interaction hub.

    Evidence Crystal structure of the DDX6 RecA-like domain bound to the EDC3 FDF peptide, competition assays, and Me31B FDF-surface mutagenesis with P-body and repression readouts

    PMID:19285948

    Open questions at the time
    • Functional consequence of switching between EDC3 and Tral occupancy in vivo not resolved
    • How competition is regulated temporally unknown
  5. 2014 High

    Structural and biochemical definition of the CNOT1 MIF4G–DDX6 RecA2 interface revealed how miRNA target recognition is physically coupled to the CCR4-NOT repression/decay machinery, mirroring eIF4A–eIF4G.

    Evidence Crystal structures of the DDX6–CNOT1 MIF4G complex, ATPase stimulation assays, structure-guided mutagenesis, and miRNA reporter assays, independently replicated and confirmed by Co-IP in human cells

    PMID:24768538 PMID:24768540 PMID:25035296

    Open questions at the time
    • How CNOT1 binding integrates with simultaneous FDF-partner engagement not fully resolved
    • Dynamics of conformational activation in cells not measured
  6. 2015 High

    A ternary 4E-T/DDX6/CNOT1 structure distinguished 4E-T's stable, CNOT1-independent binding from the displaceable FDF partners, refining the architecture of repressive mRNPs.

    Evidence 2.1-Å crystal structure of the 4E-T CHD/DDX6/CNOT1 MIF4G complex with in vitro competition assays

    PMID:26489469

    Open questions at the time
    • In vivo stoichiometry of the ternary complex not measured
    • How 4E-T persistence affects partner exchange in cells unresolved
  7. 2015 Medium

    Systematic interactome mapping placed DDX6 in distinct decapping, CPEB-like, and Ataxin2 complexes and defined a minimal P-body assembly module of DDX6/4E-T/LSM14A separable from decay.

    Evidence Tandem affinity purification mass spectrometry and P-body assembly assays with component knockdowns

    PMID:25995375

    Open questions at the time
    • Which complexes are simultaneous versus mutually exclusive not resolved
    • Functional output of each complex not individually dissected
  8. 2019 High

    Demonstrating that P-body dissolution upon DDX6 loss de-represses fate-instructive factors connected DDX6-driven translational sequestration to control of the pluripotent state and chromatin landscape.

    Evidence DDX6 knockout in human and mouse ESCs with polysome profiling, ATAC-seq, ChIP-seq, methylation profiling, and P-body imaging

    PMID:31588046

    Open questions at the time
    • Which specific repressed mRNAs are causal for the phenotype not pinpointed
    • Direct versus indirect chromatin effects not separated
  9. 2019 Medium

    Identification of de novo RecA2-domain missense variants causing P-body and partner-binding defects established DDX6 as a human disease gene and validated RecA2 integrity in vivo.

    Evidence Patient-derived cell P-body assays, IP of DDX6 variants, complementation, and structural modeling

    PMID:31422817

    Open questions at the time
    • Causal mRNA dysregulation underlying clinical phenotype not defined
    • Genotype-phenotype correlation across variants limited
  10. 2017 High

    A genome-wide screen and epistasis showed DDX6 restrains interferon-stimulated gene overactivation through the LSM1 mRNA-degradation pathway rather than canonical RNA sensors, defining an innate-immune surveillance role.

    Evidence Genome-wide genetic screen, DDX6 knockout transcriptomics, and double-knockout epistasis with LSM1 and RNA sensors

    PMID:28746868

    Open questions at the time
    • Specific ISG-encoding transcripts targeted not identified
    • Reconciliation with reported RIG-I-enhancing role unresolved
  11. 2022 High

    Parallel knockout epistasis in mouse embryos showed DDX6's essential early developmental function operates via the miRNA pathway, not its P-body or decapping arms, separating its multiple activities genetically.

    Evidence Ddx6, Dgcr8, Dcp2, and Eif4enif1 knockout mouse models with EpiLC differentiation and transcriptome epistasis

    PMID:36197846

    Open questions at the time
    • Whether the same partitioning holds in other tissues unknown
    • Direct miRNA targets controlling BMP inhibitors not fully enumerated
  12. 2024 High

    Ribosome profiling defined a mechanism by which DDX6, via its RecA2 FDF motif on the ribosome and its ATPase activity, selectively destabilizes inefficiently translated mRNAs, linking translation status to decay.

    Evidence Ribosome profiling, RNA-seq, FDF-motif and ATPase mutagenesis, and reporter mRNA assays

    PMID:38989862

    Open questions at the time
    • How DDX6 senses translation inefficiency mechanistically not resolved
    • Direct deadenylase coupling at the ribosome not visualized
  13. 2024 High

    Demonstrating DDX6-driven liquid-liquid phase separation into P-body reservoirs that store low-GC mRNAs tied granule biophysics to metabolic reprogramming and drug sensitivity in cancer.

    Evidence CRISPR screen, DDX6 KO, RIP-seq, RNA-seq, polysome profiling, and metabolic and drug-sensitivity assays in AML

    PMID:41330929

    Open questions at the time
    • Sequence/structural determinants of LLPS within DDX6 not mapped
    • Whether storage versus decay fate is reversible not resolved
  14. 2025 High

    Identifying a DDX6–DDX3X interaction required to block 48S preinitiation complex assembly defined the molecular step at which DDX6 represses translation of miRNA targets.

    Evidence DDX3X conditional knockout, DDX6/DDX3X Co-IP, interaction-defective mutant rescue in ESCs, and in vitro 48S assembly assay

    PMID:40923767

    Open questions at the time
    • Whether DDX3X recruitment is constitutive or regulated unknown
    • Structural basis of the C-terminal DDX6–DDX3X contact not resolved
  15. 2025 Medium

    Discovery of non-proteolytic polyubiquitylation of DDX6 by praja2 downstream of cAMP/GPCR signaling provided a signal-responsive switch governing P-body assembly and translational output.

    Evidence Co-IP of praja2/DDX6, ubiquitylation-defective mutant expression, polysome profiling, cAMP stimulation, and GBM proliferation assays

    PMID:40148504

    Open questions at the time
    • Ubiquitylated lysine residues and chain topology not mapped
    • How ubiquitin modification alters DDX6 partner binding mechanistically unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How DDX6's distinct molecular activities—miRNA repression, ribosome-associated decay, granule phase separation, and innate-immune surveillance—are dynamically partitioned and switched within a single cell remains unresolved.
  • No integrated model of how competing partner occupancy is regulated in real time
  • Determinants directing a bound mRNP toward storage versus decay versus reactivation unknown
  • Post-translational regulation beyond praja2 ubiquitylation uncharacterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 5 GO:0140098 catalytic activity, acting on RNA 4 GO:0140657 ATP-dependent activity 4 GO:0045182 translation regulator activity 3 GO:0060090 molecular adaptor activity 3
Localization
GO:0005829 cytosol 4 GO:0005634 nucleus 3 GO:0005840 ribosome 2
Pathway
R-HSA-1266738 Developmental Biology 3 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-8953854 Metabolism of RNA 3 R-HSA-168256 Immune System 2
Partners
AGO2ATXN2CNOT1DDX3XEDC3EIF4ENIF1LSM14AYBX1
Complex memberships
CCR4-NOT complexP-bodyRISCmRNA decapping complex

Evidence

Reading pass · 45 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2006 RCK/p54 (DDX6) directly interacts with Argonaute proteins Ago1 and Ago2 in affinity-purified active siRISC and miRISC from human cells. Depletion of RCK/p54 disrupts P-bodies, disperses Ago2 throughout the cytoplasm, releases miRNA-induced translational repression, but does not significantly affect siRNA-mediated RNA cleavage functions of RISC. Affinity purification of active RISC complexes, co-immunoprecipitation, siRNA knockdown with translational repression assays PLoS biology High 16756390
2014 The CNOT1 MIF4G domain directly recruits DDX6 through interaction with the C-terminal RecA2 domain of DDX6. Crystal structure of the DDX6-CNOT1 MIF4G complex shows striking similarity to the eIF4G-eIF4A complex. CNOT1 modulates the conformation of DDX6 and stimulates its ATPase activity. Structure-based mutations disrupting the CNOT1 MIF4G-DDX6 interaction impair miRNA-mediated repression. Crystal structure determination, ATPase activity assays, structure-guided mutagenesis, miRNA repression assays Molecular cell High 24768538 24768540
2014 The CNOT1 MIF4G domain interacts with the C-terminal RecA2 domain of DDX6; the crystal structure of this complex demonstrates that DDX6 binds CNOT1 in a manner analogous to eIF4A binding eIF4G. This interaction connects miRNA target recognition to translational repression and decapping. Crystal structure of DDX6 RecA2–CNOT1 MIF4G complex, biochemical binding assays Molecular cell High 24768540
2009 The crystal structure of the DDX6 C-terminal RecA-like domain bound to the FDF motif of decapping activator EDC3 was determined. The FDF peptide adopts an alpha-helical conformation occupying a shallow groove on DDX6 opposite to the RNA-binding and ATP hydrolysis surfaces. The translational repressor Tral contains a similar FDF motif that binds the same surface, making EDC3 and Tral interactions with DDX6/Me31B mutually exclusive. Mutagenesis of Me31B's FDF interaction surface abrogates P-body accumulation and mRNA repression. Crystal structure, mutagenesis of Me31B, competition binding assays, P-body accumulation assays, mRNA repression assays Molecular cell High 19285948
2015 The 2.1-Å crystal structure of a ternary 4E-T CHD/DDX6/CNOT1 MIF4G complex reveals how the 4E-T CUP-homology domain wraps around the RecA2 domain of DDX6 and contacts CNOT1. Unlike Edc3 and Pat1 FDF motifs, which dissociate from DDX6 upon CNOT1 MIF4G binding in vitro, 4E-T CHD interacts with DDX6 in both the presence and absence of CNOT1. X-ray crystallography (2.1 Å), in vitro binding competition assays Cell reports High 26489469
2014 Direct interaction between CNOT1 (large scaffolding subunit of CCR4-NOT) and DDX6 is required for miRISC-mediated gene silencing in human cells. DDX6 binds a conserved CNOT1 subdomain analogous to the eIF4A-eIF4G interaction; mutations disrupting this interaction impair miRNA silencing. Co-immunoprecipitation, in vitro binding with purified proteins, mutagenesis, miRNA silencing reporter assays in human cells RNA (New York, N.Y.) High 25035296
2016 The DDX6–4E-T interaction mediates both miRNA-dependent translational repression and de novo P-body assembly. Joint deletion of two short conserved motifs in 4E-T that bind UNR and DDX6 relieves translational repression, partly through the 4E-T–DDX6–CNOT1 axis. Mass spectrometry to map interaction sites, deletion mutagenesis, translational repression reporter assays, P-body assembly microscopy Nucleic acids research High 27342281
2003 Recombinant rck/p54 specifically binds c-myc RNA transcripts and exhibits ATP-dependent RNA-unwinding activity toward c-myc RNAs in vitro. The C-terminal 184 amino acid domain (aa 289–472) is required for RNA unwinding activity. Surface plasmon resonance RNA binding assay, in vitro helicase/unwinding assay, deletion mutagenesis Genes to cells Medium 12875652
2006 Crystal structure of the N-terminal core domain (Nc-rck/p54, residues 70–288) reveals a P-loop in motif I adopting a closed conformation induced by Asn131, a residue unique to the RCK subfamily, such that ATP does not bind this loop in the unliganded state. ATP induces a conformational change bringing N- and C-terminal domains together for RNA unwinding. c-Myc IRES RNA is a substrate for rck/p54-mediated unwinding, and overexpression of rck/p54 in HeLa cells causes G2/M arrest with downregulation of c-myc. X-ray crystallography, dynamic light scattering, in vitro helicase assay, electron microscopy, luciferase IRES assay, cell cycle analysis Genes to cells High 16611246
2002 Xp54 (DDX6 ortholog) shuttles between nucleus and cytoplasm via a leucine-rich nuclear export signal (NES) recognized by the CRM1 pathway (blocked by leptomycin B). Xp54 binds nascent transcripts on lampbrush chromosome loops in the nucleus and accompanies mRNA to the cytoplasm by an alternative export pathway, remaining associated with masked mRNA until translational activation. Microinjection of recombinant protein in oocytes, leptomycin B treatment, NES mutagenesis, immunostaining of lampbrush chromosomes, co-immunoprecipitation with de novo RNA Journal of cell science Medium 11839790
2017 DDX6 localizes to both nucleus and cytoplasm in human cells. Nuclear import of DDX6 can be mediated in a piggyback manner by 4E-T, rather than by a canonical NLS/importin-α/β pathway. DDX6 also enters newly formed nuclei by associating ('hitch-hiking') with mitotic chromosomes via its C-terminal domain during M phase. Live-cell imaging, fractionation, co-immunoprecipitation, mitotic chromosome association assays, domain deletion analysis Scientific reports Medium 28216671
2010 DDX6 interacts with hnRNP K and hnRNP E1 in a DICE (differentiation control element)-dependent manner in erythroid K562 cells. DDX6 colocalizes with endogenous hr15-LOX mRNA in P-body-like RNP granules from which 60S ribosomal subunits are excluded. DDX6 knockdown disrupts storage of hr15-LOX mRNA in these granules, indicating DDX6 maintains translational silencing by recruiting silenced mRNA to P-body-like storage compartments. RNA chromatography with DICE as bait, hnRNP K immunoprecipitation, RNAi knockdown, FISH co-localization, ribosomal fractionation RNA (New York, N.Y.) Medium 20884783
2011 DDX6 binds the DB1 and DB2 stem-loop structures in the dengue virus 3' UTR in vitro, and this interaction occurs in vivo during DENV infection. DDX6 knockdown reduces infectious particle production and viral RNA levels. DDX6 colocalizes with the DENV replication complex. RNA chromatography with quantitative mass spectrometry, in vitro RNA-protein binding assays, siRNA knockdown, immunofluorescence co-localization RNA biology Medium 21957497
2012 DDX6 helicase activity (DEAD-box motif II) is required for efficient HCV replication. A helicase-deficient DQAD mutant has a dominant-negative effect reducing HCV yields, whereas overexpression of DDX6 enhances replication. An intracellular complex containing DDX6, HCV core protein, and both viral and cellular RNAs is formed in a C-terminal domain-dependent but helicase-activity-independent manner. siRNA knockdown and rescue with siRNA-resistant mutant, overexpression of helicase-dead (DQAD) mutant, co-immunoprecipitation Journal of virology Medium 20392846
2012 DDX6 acts enzymatically (ATPase/helicase activity required) to facilitate HIV-1 capsid assembly, specifically at the step of Gag multimerization at the plasma membrane. Assembling HIV-1 co-opts a preexisting host complex containing ABCE1 and PB proteins including DDX6. DDX6 knockdown reduces production of infectious HIV-1 from primary human T cells. Co-immunoprecipitation, immunoelectron microscopy, siRNA knockdown with rescue, localization of assembly defect by IEM The Journal of cell biology Medium 22851315
2011 DDX6 ATPase/helicase motif is essential for foamy virus genome packaging. During infection, DDX6 relocalizes from P-bodies/stress granules to the pericentriolar viral assembly site. DDX6 does not stably interact with Gag proteins and is not incorporated into virions, suggesting it transiently remodels viral RNA–Gag RNP to facilitate genome encapsidation. siRNA knockdown, ATPase mutant rescue, immunofluorescence relocalization, viral RNA quantification in particles PLoS pathogens Medium 22022269
2014 DDX6 interacts with CUG triplet-repeat RNA in primary fibroblasts from DM1 patients and unwinds CUG-repeat duplexes in vitro in an ATP-dependent manner. DDX6 overexpression causes relocation of CUG-expanded DMPK-mRNA foci from nucleus to cytoplasm and dispersal of MBNL1, relieving DM1 mis-splicing. Knockdown of DDX6 increases nuclear DMPK-mRNA foci and MBNL1 sequestration. RIP (RNA immunoprecipitation) in patient fibroblasts, in vitro CUG-RNA unwinding assay, DDX6 overexpression and knockdown with splicing assays, immunofluorescence Nucleic acids research Medium 24792155
2015 DDX6 associates with YBX1 to bind stem loops in the 3' UTRs of CDK1 and EZH2 mRNAs and recruits them to eIF4E to facilitate translation, maintaining progenitor self-renewal. DDX6 also associates with mRNA degradation proteins to degrade KLF4 mRNA (differentiation inducer) via its 5' UTR. Loss of DDX6 in epidermal progenitor cells causes premature differentiation and decreased proliferation. Co-immunoprecipitation with YBX1, mRNA pull-down, polysome profiling, RNAi knockdown in epidermal progenitors with differentiation/proliferation phenotypic readouts Molecular cell Medium 26412305
2019 DDX6 mediates translational suppression of target mRNAs in P-bodies; loss of DDX6 dissolves P-bodies, releasing mRNAs encoding fate-instructive transcription and chromatin factors into the ribosome pool. Increased translation of these targets rewires enhancer, heterochromatin, and DNA methylation landscapes, endowing primed ESCs with a hyper-pluripotent, differentiation-resistant state. DDX6 knockdown/knockout in human and mouse ESCs, polysome profiling, ATAC-seq, ChIP-seq, DNA methylation profiling, P-body imaging Cell stem cell High 31588046
2019 De novo missense variants in the RecA-2 domain of DDX6 (p.His372Arg, p.Arg373Gln, p.Cys390Arg, p.Thr391Ile, p.Thr391Pro) cause P-body assembly defects and disrupt interactions with protein partners in immunoprecipitation assays, establishing that RecA-2 domain integrity is required for P-body assembly and partner binding in human cells. Patient-derived fibroblast and cell line P-body assembly assays, immunoprecipitation of DDX6 variants, complementation assays, structural modeling American journal of human genetics Medium 31422817
2013 Recombinant DDX6 inhibits VEGF IRES-mediated translation in normoxic MCF-7 cell extracts. DDX6 interacts with the VEGF mRNA 5' UTR by RNA affinity chromatography. Under hypoxia, DDX6 protein levels decline and its interaction with VEGF mRNA is diminished; DDX6 depletion by RNAi further promotes VEGF expression and vascular tube formation. In vitro IRES-translation assay with recombinant DDX6, RNA affinity chromatography/mass spectrometry, RNAi knockdown, VEGF secretion and HUVEC tube formation assay The Journal of biological chemistry Medium 23293030
2017 DDX6 deficiency causes global upregulation of interferon-stimulated genes (ISGs) in a cell-intrinsic manner, imposing an antiviral state. Epistatic analysis showed ISG activation cannot be overcome by deletion of canonical RNA sensors, but DDX6 deficiency is suppressed by disrupting LSM1 (mRNA degradation machinery component), placing DDX6 in a pathway that limits aberrant ISG activation through RNA degradation. Genome-wide genetic screen, DDX6 knockout with transcriptomics, epistasis analysis (double knockout with LSM1 and RNA sensors), viral infection assays Cell reports High 28746868
2018 DDX6 associates with RIG-I; their interaction increases after influenza B virus infection. DDX6 augments RIG-I-mediated induction of IFN-β expression. DDX6 binds viral RNA capable of stimulating RIG-I, suggesting it functions as an RNA co-sensor and signaling enhancer for RIG-I. Affinity purification/quantitative mass spectrometry, co-immunoprecipitation, IFN-β reporter assay, viral RNA binding assay International journal of molecular sciences Medium 29949917
2024 DDX6 limits stress granule formation in an ATPase and RNA-binding dependent manner, independent of P-body formation. Loss of DDX6 (along with 4E-T and DCP1A) increases P-body docking with stress granules in a manner dependent on CNOT1 and PAT1B. DDX6 knockout, ATPase mutant rescue, stress granule and P-body live imaging, co-localization analysis, epistasis with CNOT1/PAT1B knockdowns The Journal of cell biology Medium 38536035
2024 DDX6 triggers deadenylation-dependent decay of inefficiently translated mRNAs. DDX6 interacts with the ribosome through its RecA2-domain FDF motif. Both RecA2-mediated interactions and ATPase activity are required for DDX6 to destabilize inefficiently translated mRNAs. Ribosome profiling identifies two classes of DDX6-regulated endogenous mRNAs: those with poor overall translation efficiency and those with locally reduced ribosome translocation rates. Ribosome profiling, RNA sequencing, FDF-motif mutagenesis, reporter mRNA assays, ATPase mutant analysis eLife High 38989862
2023 DDX6 helicase activity (E247A mutant is inactive) is required for maintaining proper separation of P-bodies from stress granules under stress. DDX6 deficiency results in formation of irregular 'hybrid' PB/SG granules. The interactions of DDX6 with CNOT1 and 4E-T modulate both P-body and stress granule biogenesis. DDX6 knockout cell lines, helicase-dead E247A mutant rescue, fluorescence microscopy of PB and SG markers, CNOT1/4E-T knockdown epistasis Nucleic acids research Medium 37427791
2015 DDX6 interactome by TAP-MS identifies three main complexes: the decapping complex, a CPEB-like complex, and an Ataxin2/Ataxin2L complex; the exon junction complex (EJC) was also found, suggesting DDX6 binding to newly exported mRNAs. Some DDX6 associates with polysomes. P-body assembly requires DDX6 together with 4E-T and LSM14A in all tested conditions, whereas decay complex proteins are dispensable for P-body assembly per se. Tandem affinity purification (TAP) with mass spectrometry, P-body assembly assays under multiple conditions, knockdown of individual components Molecular biology of the cell Medium 25995375
2012 Rck/p54 (DDX6) binds RNA with no sequence specificity and high nanomolar affinity. RNA binding is ATP-independent, but relaxing (unwinding) of bound RNA requires ATP (not ATP hydrolysis). Rck/p54 is in large molecular excess relative to cellular mRNAs and is enriched to ~0.5 mM in P-bodies, organized in clusters, with multiple Rck/p54 proteins binding along individual mRNA molecules in vivo. In vitro RNA binding assays with purified protein, RNA conformation assays, quantitative immunofluorescence of P-bodies, in vivo crosslinking RNA (New York, N.Y.) Medium 22836354
2016 DDX6 binds 7SK snRNA and causes release and transfer of P-TEFb from 7SK snRNP to the AF4/AFF1 super elongation complex (SEC). DDX6 binds stably to AF4 and AF4N as demonstrated by GST pull-down and co-immunoprecipitation. Overexpression of DDX6 increases cellular mRNA production ~5–6 fold; knockdown of DDX6 decreases mRNA production by ~70%. 7SK snRNA binding assay, GST pull-down, co-immunoprecipitation, mRNA production assay with overexpression and knockdown American journal of blood research Low 27679741
2017 In Drosophila circadian pacemaker neurons, ME31B/DDX6 is required for ATX2 association with NOT1, enabling NOT1-mediated gene silencing. The ME31B/DDX6–NOT1 complex supports high-amplitude behavioral rhythms independently of PERIOD (PER) translation. LSM12 separately acts as a molecular adaptor recruiting TYF to ATX2 for TYF-dependent PER translation. Genetic interaction assays in Drosophila, co-immunoprecipitation of ATX2/NOT1/ME31B, behavioral rhythm analysis, molecular epistasis Molecular cell Medium 28388438
2007 Ataxin-2 directly interacts with DDX6, a component of P-bodies and stress granules. Altered ataxin-2 levels interfere with assembly of stress granules and P-bodies. Ataxin-2 also regulates the intracellular concentration of poly(A)-binding protein (PABP), another interaction partner. Co-immunoprecipitation, overexpression/knockdown of ataxin-2, immunofluorescence of P-bodies and stress granules Molecular biology of the cell Medium 17392519
2024 PolyQ-expanded (PQE) ataxin-2 sequesters DDX6 into aggregates via RNA sequences. The N-terminal LSm domain of ataxin-2 (residues 82–184) and the C-terminal helicase domain of DDX6 mediate this interaction. Sequestration of DDX6 impairs P-body assembly, releasing MARF1 endoribonuclease and promoting mRNA decay and translational repression, which can be rescued by restoring DDX6 protein levels. Biochemical fractionation, fluorescence imaging, domain mapping by Co-IP with truncation mutants, rescue experiments by DDX6 restoration The Journal of biological chemistry Medium 38810698
2022 In early mouse embryogenesis, DDX6 prevents aberrant upregulation of BMP signaling inhibitors through miRNA-mediated gene silencing. Ddx6 knockout phenocopies Dgcr8 (miRNA pathway) knockout but not Dcp2 (decapping) or Eif4enif1 (4E-T, P-body) knockout, establishing that DDX6's early developmental function is primarily via the miRNA pathway rather than P-body-related functions. Ddx6 KO mice, Dgcr8 KO, Dcp2 KO, Eif4enif1 KO mouse models, in vitro EpiLC differentiation assay, transcriptome analysis, genetic epistasis PLoS genetics High 36197846
2021 DDX6 is a positive regulator of the Ataxin-2/PAPD4 cytoplasmic polyadenylation machinery. DDX6 interacts with Ataxin-2, PABPC1, and PAPD4 in immunoprecipitation assays. DDX6 downregulation increases Ataxin-2 target mRNAs with short poly(A) tails and reduces their protein levels, similar to Ataxin-2 downregulation. DDX6 likely promotes binding of Ataxin-2 to target mRNAs to maintain poly(A) tail length. LC-MS/MS interactome analysis, Co-IP, siRNA knockdown of DDX6/Ataxin-2, poly(A) tail length assay Biochemical and biophysical research communications Low 33756349
2025 DDX6 interacts directly with DDX3X via its C-terminal region; this interaction is required for DDX6-mediated translational repression in miRNA-mediated silencing. DDX6 mutant defective in DDX3X interaction cannot rescue miRNA silencing defects in ESCs and fails to inhibit 48S preinitiation complex formation in vitro. Conditional knockout of DDX3X, biochemical Co-IP of DDX6/DDX3X, DDX6 interaction-defective mutant rescue assay, in vitro 48S preinitiation complex assembly assay Nucleic acids research High 40923767
2025 E3 ubiquitin ligase praja2 forms a complex with DDX6 and promotes P-body assembly through non-proteolytic polyubiquitylation of DDX6 upon cAMP/GPCR signaling. Expression of ubiquitylation-defective DDX6 mutant suppresses P-body assembly and sustains GBM growth. Genetic inactivation of praja2 reshapes DDX6/mRNA complexes and translating polysomes, promoting cellular senescence and GBM growth arrest. Co-immunoprecipitation of praja2/DDX6 complex, ubiquitylation-defective mutant expression, polysome profiling, cAMP stimulation, GBM cell proliferation assays EMBO reports Medium 40148504
2001 Overexpression of rck/p54 in COS7 and SW480 colorectal cancer cells causes an increase in c-myc protein levels by enhancing translation efficiency and/or stabilizing c-myc mRNA. Transfection overexpression, Western blot, translational efficiency assessment Carcinogenesis Low 11751426
2021 DDX6 binds the dengue virus 3' UTR A3 element RNA hairpin with nanomolar affinity; three conserved basic residues (Lys307, Lys367, Arg369) and an unstructured C-terminal extension are required. Alanine substitution of these residues results in RNA-independent ATPase activity, indicating RNA binding and ATPase activities are coupled. DDX6-mediated interaction with DENV A3 element contributes to G1/S cell cycle arrest during infection; DDX6 overexpression rescues DNA preinitiation complex expression. Binding affinity measurements, mutagenesis of DDX6 basic residues, DDX6 CLIP in infected cells, ATPase activity assay, cell cycle analysis, rescue of DNA replication complex Journal of virology Medium 34132569
2024 DDX6 undergoes liquid-liquid phase separation (LLPS) to form P-bodies that serve as 'reservoirs' for DDX6-bound mRNAs with low GC content. DDX6 KO leads to P-body dissolution and release of sequestered mRNAs (including BCAT1) into the cytosol for degradation, thereby reprogramming amino acid metabolism and sensitizing AML cells to cytarabine. In vitro and in vivo CRISPR screen, DDX6 KO, RIP-seq, RNA-seq, polysome profiling, metabolic assays, drug sensitivity assays Nature communications High 41330929
2024 DDX6 is strongly associated with NF-κB pathway components p65/RelA and IκBα (but not TRADD, RIP, or TRAF2) by co-immunoprecipitation. Forced expression of DDX6 enhances NF-κB promoter activity independent of its RNA helicase activity. DDX6 silencing reduces TNF-α-induced phosphorylation of p65 and IκBα, nuclear localization of p65, and IκBα protein levels. Co-immunoprecipitation, NF-κB reporter assay with helicase-inactive mutant, siRNA knockdown, immunofluorescence of p65 nuclear localization, imiquimod dermatitis mouse model Biochemical and biophysical research communications Medium 38377944
2024 ADAR1 interacts with DDX6 in the nucleus. DDX6 functions as a negative regulator of cellular ADAR1p110 and ADAR2 editing activity, as assessed by a dual-fluorescence reporter assay. Depletion of DDX6 facilitates RA-induced neuronal differentiation. Nuclear fraction mass spectrometry/immunoprecipitation, dual-fluorescence RNA editing reporter assay, DDX6 knockdown in neuronal differentiation model International journal of molecular sciences Low 36834609
2024 NMDAR stimulation causes S387 phosphorylation of Ago2, which recruits DDX6 to RISC. DDX6 recruitment to RISC is required for NMDAR-dependent silencing of Limk1 mRNA via miR-134 (but not Apt1 via miR-138), and is essential for NMDAR-dependent dendritic spine shrinkage. Ago2 S387 phosphorylation assays, DDX6 knockdown in neurons, mRNA-specific RISC RIP, dendritic spine morphology assays Scientific reports Medium 38321143
2023 In Drosophila germline, mutations in the helicase domain, N-terminal domain, C-terminal domain, and FDF-binding motif of Me31B/DDX6 by CRISPR cause distinct defects in fertility, oogenesis, embryo patterning, and germline mRNA regulation, demonstrating that different motifs contribute distinct functions in vivo. CRISPR mutagenesis of specific motifs, Drosophila germline phenotype analysis, mRNA regulation assays FEBS letters Medium 37235728
2019 DDX6 is required for NANOS2 localization and function in mouse male germ cells. DDX6-null germ cells show both P-body-dependent and P-body-independent defects compared to NANOS2-null germ cells, demonstrating that NANOS2 function is carried out via both DDX6/P-body-dependent and -independent mechanisms. Germ cell-specific Cre-mediated Ddx6 deletion in chimeric embryos, comparative phenotype analysis vs. NANOS2-null, RNA-seq Scientific reports Medium 30679547
2023 DDX6 antagonizes PI3K-AKT signaling in mouse oocytes to maintain primordial follicles. Oocyte-specific Ddx6 knockout causes P-body-like granule disassembly, abnormal oocyte enlargement via enhanced PI3K-AKT signaling, and premature depletion of primordial follicles. Conversely, forced activation of PI3K-AKT (by Pten KO) disassembles P-body-like granules, supporting mutual antagonism between DDX6/P-bodies and PI3K-AKT signaling. Oocyte-specific Cre (Gdf9-iCre) conditional Ddx6 KO mice, Pten KO epistasis, immunofluorescence of P-body markers, follicle counting Biology of reproduction Medium 37067907

Source papers

Stage 0 corpus · 98 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. PLoS biology 413 16756390
2007 Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and interferes with P-bodies and stress granules. Molecular biology of the cell 281 17392519
2014 Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression. Molecular cell 259 24768538
2014 A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. Molecular cell 237 24768540
2015 P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes. Molecular biology of the cell 153 25995375
2011 Quantitative mass spectrometry of DENV-2 RNA-interacting proteins reveals that the DEAD-box RNA helicase DDX6 binds the DB1 and DB2 3' UTR structures. RNA biology 153 21957497
2006 Xp54 and related (DDX6-like) RNA helicases: roles in messenger RNP assembly, translation regulation and RNA degradation. Nucleic acids research 142 16769775
2019 The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis. Cell stem cell 121 31588046
2014 Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1. RNA (New York, N.Y.) 108 25035296
2009 Structural basis for the mutually exclusive anchoring of P body components EDC3 and Tral to the DEAD box protein DDX6/Me31B. Molecular cell 106 19285948
2016 The DDX6-4E-T interaction mediates translational repression and P-body assembly. Nucleic acids research 104 27342281
2010 DDX6 (Rck/p54) is required for efficient hepatitis C virus replication but not for internal ribosome entry site-directed translation. Journal of virology 91 20392846
2015 Structure of a Human 4E-T/DDX6/CNOT1 Complex Reveals the Different Interplay of DDX6-Binding Proteins with the CCR4-NOT Complex. Cell reports 90 26489469
2015 DDX6 Orchestrates Mammalian Progenitor Function through the mRNA Degradation and Translation Pathways. Molecular cell 82 26412305
1995 The rck/p54 candidate proto-oncogene product is a 54-kilodalton D-E-A-D box protein differentially expressed in human and mouse tissues. Cancer research 81 7614484
2012 Multiple binding of repressed mRNAs by the P-body protein Rck/p54. RNA (New York, N.Y.) 79 22836354
2012 HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly. The Journal of cell biology 74 22851315
2007 DjCBC-1, a conserved DEAD box RNA helicase of the RCK/p54/Me31B family, is a component of RNA-protein complexes in planarian stem cells and neurons. Developmental dynamics : an official publication of the American Association of Anatomists 74 17994545
1999 Overexpression of rck/p54, a DEAD box protein, in human colorectal tumours. British journal of cancer 70 10360675
2020 DHH1/DDX6-like RNA helicases maintain ephemeral half-lives of stress-response mRNAs. Nature plants 67 32483330
2011 MicroRNA 130 family regulates the hypoxia response signal through the P-body protein DDX6. Nucleic acids research 61 21486751
2002 RNA helicase p54 (DDX6) is a shuttling protein involved in nuclear assembly of stored mRNP particles. Journal of cell science 61 11839790
2017 DDX6 Represses Aberrant Activation of Interferon-Stimulated Genes. Cell reports 58 28746868
2017 LSM12 and ME31B/DDX6 Define Distinct Modes of Posttranscriptional Regulation by ATAXIN-2 Protein Complex in Drosophila Circadian Pacemaker Neurons. Molecular cell 54 28388438
2013 DDX6 post-transcriptionally down-regulates miR-143/145 expression through host gene NCR143/145 in cancer cells. Biochimica et biophysica acta 51 23932921
2001 Co-overexpression of DEAD box protein rck/p54 and c-myc protein in human colorectal adenomas and the relevance of their expression in cultured cell lines. Carcinogenesis 51 11751426
2011 The DEAD-box RNA helicase DDX6 is required for efficient encapsidation of a retroviral genome. PLoS pathogens 50 22022269
2015 Positive feedback of DDX6/c-Myc/PTB1 regulated by miR-124 contributes to maintenance of the Warburg effect in colon cancer cells. Biochimica et biophysica acta 49 26144048
2014 DDX6 and its orthologs as modulators of cellular and viral RNA expression. Wiley interdisciplinary reviews. RNA 48 24788243
2015 The RNA helicase DDX6 regulates cell-fate specification in neural stem cells via miRNAs. Nucleic acids research 47 25722370
2013 Identification of DEAD-box RNA helicase 6 (DDX6) as a cellular modulator of vascular endothelial growth factor expression under hypoxia. The Journal of biological chemistry 47 23293030
2019 Rare De Novo Missense Variants in RNA Helicase DDX6 Cause Intellectual Disability and Dysmorphic Features and Lead to P-Body Defects and RNA Dysregulation. American journal of human genetics 45 31422817
2018 The RNA Helicase DDX6 Associates with RIG-I to Augment Induction of Antiviral Signaling. International journal of molecular sciences 45 29949917
2014 DDX6 regulates sequestered nuclear CUG-expanded DMPK-mRNA in dystrophia myotonica type 1. Nucleic acids research 42 24792155
2008 Knockdown of RCK/p54 expression by RNAi inhibits proliferation of human colorectal cancer cells in vitro and in vivo. Cancer biology & therapy 38 18769115
2003 Overexpression of a DEAD box/RNA helicase protein, rck/p54, in human hepatocytes from patients with hepatitis C virus-related chronic hepatitis and its implication in hepatocellular carcinogenesis. Journal of viral hepatitis 35 12823589
2018 Oncogene RNA helicase DDX6 promotes the process of c-Myc expression in gastric cancer cells. Molecular carcinogenesis 34 29314290
2010 DDX6 recruits translational silenced human reticulocyte 15-lipoxygenase mRNA to RNP granules. RNA (New York, N.Y.) 34 20884783
2024 DDX6 modulates P-body and stress granule assembly, composition, and docking. The Journal of cell biology 30 38536035
2013 Modulation of hepatitis C virus RNA accumulation and translation by DDX6 and miR-122 are mediated by separate mechanisms. PloS one 30 23826300
2003 A tumour-associated DEAD-box protein, rck/p54 exhibits RNA unwinding activity toward c-myc RNAs in vitro. Genes to cells : devoted to molecular & cellular mechanisms 28 12875652
2015 Functional analysis of the 11q23.3 glioma susceptibility locus implicates PHLDB1 and DDX6 in glioma susceptibility. Scientific reports 26 26610392
2023 RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules. Nucleic acids research 24 37427791
2006 Human DEAD-box/RNA unwindase rck/p54 contributes to maintenance of cell growth by affecting cell cycle in cultured cells. International journal of oncology 24 16773183
2017 Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6. Scientific reports 23 28216671
2019 Synthetic miR-143 Inhibits Growth of HER2-Positive Gastric Cancer Cells by Suppressing KRAS Networks Including DDX6 RNA Helicase. International journal of molecular sciences 22 30959742
2018 DEAD-Box Protein RNA-Helicase DDX6 Regulates the Expression of HER2 and FGFR2 at the Post-Transcriptional Step in Gastric Cancer Cells. International journal of molecular sciences 21 29987267
2006 Structural insight of human DEAD-box protein rck/p54 into its substrate recognition with conformational changes. Genes to cells : devoted to molecular & cellular mechanisms 21 16611246
2005 Expression of rck/p54, a DEAD-box RNA helicase, in gametogenesis and early embryogenesis of mice. Developmental dynamics : an official publication of the American Association of Anatomists 21 15906376
1995 Cloning and expression of a murine cDNA homologous to the human RCK/P54, a lymphoma-linked chromosomal translocation junction gene on 11q23. Gene 21 8543178
2018 Tau/DDX6 interaction increases microRNA activity. Biochimica et biophysica acta. Gene regulatory mechanisms 20 29966762
2017 Cellular DEAD-box RNA helicase DDX6 modulates interaction of miR-122 with the 5' untranslated region of hepatitis C virus RNA. Virology 19 28456022
2016 DDX6 transfers P-TEFb kinase to the AF4/AF4N (AFF1) super elongation complex. American journal of blood research 19 27679741
2011 The adenovirus E4 11 k protein binds and relocalizes the cytoplasmic P-body component Ddx6 to aggresomes. Virology 19 21700307
2024 PolyQ-expanded ataxin-2 aggregation impairs cellular processing-body homeostasis via sequestering the RNA helicase DDX6. The Journal of biological chemistry 17 38810698
2024 Human DDX6 regulates translation and decay of inefficiently translated mRNAs. eLife 17 38989862
2022 The RNA helicase DDX6 controls early mouse embryogenesis by repressing aberrant inhibition of BMP signaling through miRNA-mediated gene silencing. PLoS genetics 17 36197846
2021 LncRNA CASC19 accelerates chondrocytes apoptosis and proinflammatory cytokine production to exacerbate osteoarthritis development through regulating the miR-152-3p/DDX6 axis. Journal of orthopaedic surgery and research 16 34158095
2021 DEAD-Box Helicase DDX6 Facilitated RIG-I-Mediated Type-I Interferon Response to EV71 Infection. Frontiers in cellular and infection microbiology 16 34485180
1998 Growth inhibition by overexpression of human DEAD box protein rck/p54 in cells of a guinea pig cell line. FEBS letters 16 9662432
2020 DDX6 Helicase Behavior and Protein Partners in Human Adipose Tissue-Derived Stem Cells during Early Adipogenesis and Osteogenesis. International journal of molecular sciences 14 32283676
2017 DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells. In vitro cellular & developmental biology. Animal 14 28842848
2013 DDX6 localizes to nuage structures and the annulus of mammalian spermatogenic cells. Histochemistry and cell biology 14 24141902
2007 Xenopus Xp54 and human RCK/p54 helicases functionally replace yeast Dhh1p in brome mosaic virus RNA replication. Journal of virology 14 17301158
2022 A New Long Noncoding RNA, MAHAT, Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus by Recruiting DDX6 To Bind to ZNF34 and Promote an Innate Immune Response. Journal of virology 12 36073922
2021 Specific Interaction of DDX6 with an RNA Hairpin in the 3' UTR of the Dengue Virus Genome Mediates G1 Phase Arrest. Journal of virology 12 34132569
2018 The DEAD-box RNA-binding protein DDX6 regulates parental RNA decay for cellular reprogramming to pluripotency. PloS one 12 30273347
2023 Antagonism between DDX6 and PI3K-AKT signaling is an oocyte-intrinsic mechanism controlling primordial follicle growth†. Biology of reproduction 11 37067907
2019 ES-mediated chimera analysis revealed requirement of DDX6 for NANOS2 localization and function in mouse germ cells. Scientific reports 11 30679547
2023 miR-148a-3p and DDX6 functional link promotes survival of myeloid leukemia cells. Blood advances 10 36322827
2021 miR-152-3p aggravates vascular endothelial cell dysfunction by targeting DEAD-box helicase 6 (DDX6) under hypoxia. Bioengineered 10 34374627
2016 Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma. International journal of oncology 10 26783102
2024 DDX6 is involved in the pathogenesis of inflammatory diseases via NF-κB activation. Biochemical and biophysical research communications 9 38377944
2012 t(11;14)(q23;q32) involving IGH and DDX6 in nodal marginal zone lymphoma. Genes, chromosomes & cancer 9 22965301
2023 RNA Helicase DDX6 Regulates A-to-I Editing and Neuronal Differentiation in Human Cells. International journal of molecular sciences 8 36834609
2023 Mutational analysis of the functional motifs of the DEAD-box RNA helicase Me31B/DDX6 in Drosophila germline development. FEBS letters 8 37235728
2022 Inhibition of DDX6 enhances autophagy and alleviates endoplasmic reticulum stress in Vero cells under PEDV infection. Veterinary microbiology 8 35085948
2003 Crystallization and X-ray analysis of the N-terminal core domain of a tumour-associated human DEAD-box RNA helicase, rck/p54. Acta crystallographica. Section D, Biological crystallography 7 14684915
2024 The DDX6/KIFC1 signaling axis, as regulated by YY1, contributes to the malignant behavior of pancreatic cancer. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 6 38551642
2021 DDX6 is a positive regulator of Ataxin-2/PAPD4 cytoplasmic polyadenylation machinery. Biochemical and biophysical research communications 5 33756349
2025 Variants in the DDX6-CXCR5 autoimmune disease risk locus influence the regulatory network in immune cells and salivary gland. Annals of the rheumatic diseases 4 40447495
2024 Rab3B enhances the stabilization of DDX6 to promote lung adenocarcinoma aggressiveness. Molecular medicine (Cambridge, Mass.) 4 38834947
2021 DDX6 Is Essential for Oocyte Development and Maturation in Locusta migratoria. Insects 4 33466820
2015 Characterization and expression of DDX6 during gametogenesis in the Chinese mitten crab Eriocheir sinensis. Genetics and molecular research : GMR 4 25966215
1996 Identification and chromosome mapping of the mouse homologue of the human gene (DDX6) that encodes a putative RNA helicase of the DEAD box protein family. Cytogenetics and cell genetics 4 8995487
2024 An essential role for the RNA helicase DDX6 in NMDA receptor-dependent gene silencing and dendritic spine shrinkage. Scientific reports 3 38321143
2018 Imsnc761 and DDX6 synergistically suppress cell proliferation and promote apoptosis via p53 in testicular embryonal carcinoma cells. Bioscience reports 3 29769412
2025 Praja2 controls P-body assembly and translation in glioblastoma by non-proteolytic ubiquitylation of DDX6. EMBO reports 2 40148504
2023 Variants in the DDX6-CXCR5 autoimmune disease risk locus influence the regulatory network in immune cells and salivary gland. bioRxiv : the preprint server for biology 2 39071447
2022 Circulating Exosomal circRNA_0063476 Impairs Expression of Markers of Bone Growth Via the miR-518c-3p/DDX6 Axis in ISS. Endocrinology 2 35974445
2022 P-body dynamics revealed by DDX6 protein knockdown via the auxin-inducible degron system. Development, growth & differentiation 2 36353942
1999 Expression of two dead box genes (DDX1 and DDX6) is independent of that of MYCN in human neuroblastoma cell lines. Biochemistry and molecular biology international 2 10319407
2025 Fusidic Acid Reverses Chemoresistance in Breast Cancer via Targeting DDX6 to Downregulate GSK-3β/β-Catenin Signaling. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 1 40757496
2025 DDX6 interacts with DDX3X to repress translation in microRNA-mediated silencing. Nucleic acids research 1 40923767
2025 DDX6 undergoes phase separation to modulate metabolic plasticity and chemoresistance. Nature communications 1 41330929
2026 The host RNA helicase DDX6 restricts avian influenza virus replication by targeting viral NP and modulating ISG15. Poultry science 0 41633077
2026 DDX6-mediated CACHD1 mRNA stabilization enhances non-small cell lung cancer progression via activation of FAK signaling. Biochemical pharmacology 0 42162769
2025 Pnrc2 promotes rapid mRNA decay and coordinately supports early development with P-body factors Ddx6 and Ddx61. bioRxiv : the preprint server for biology 0 40463158

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