Affinage

CNOT1

CCR4-NOT transcription complex subunit 1 · UniProt A5YKK6

Length
2376 aa
Mass
266.9 kDa
Annotated
2026-06-09
49 papers in source corpus 32 papers cited in narrative 31 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 5/6 claims corpus-supported (83%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CNOT1 is the central scaffold of the conserved CCR4-NOT complex, a global regulator of mRNA stability and translation that originated as the essential yeast NOT1/CDC39 nuclear repressor of transcription (PMID:8428577, PMID:7926748). In human cells CNOT1 is required for CCR4-NOT deadenylase activity, mRNA decay and P-body formation, and its depletion stabilizes mRNAs in a manner correlated with codon optimality (PMID:21976065, PMID:41161383). Structurally, CNOT1 is an extended HEAT-repeat platform whose multiple MIF4G and MIF4G-like domains organize distinct functional modules: a central MIF4G domain binds CAF1/CNOT7 and positions the CCR4 nuclease with its active site accessible to RNA (PMID:22959269, PMID:22977175), a second MIF4G domain recruits the DEAD-box helicase DDX6 to couple deadenylation to translational repression and decapping (PMID:24768540, PMID:25035296), the DUF3819 domain binds CNOT9 to provide tryptophan-binding pockets for TNRC6/GW182 in miRNA silencing (PMID:24768540), the C-terminal arm recruits NOT2/NOT5 (PMID:24121231), and N-terminal helical domains sandwich CNOT10-CNOT11 to form a protein-interaction antenna (PMID:36586408). Through these interfaces CNOT1 functions as a recruitment hub for sequence-specific RNA-binding adaptors that target the complex to particular transcripts: it binds the AU-rich-element factor tristetraprolin (TTP) through a defined HEAT-like repeat surface to drive ARE-mRNA decay (PMID:21278420, PMID:40149939), engages ZFP36L1 to repress translation independently of deadenylation (PMID:32311426), and is recruited via ZFP36L1/BRF1 to Per2 mRNA to control circadian period through deadenylation-dependent decay (PMID:35510877). The CNOT1 MIF4G/DUF3819 region together with CNOT9 stimulates deadenylation of the reconstituted nuclease module (PMID:30309886), and CNOT1 also nucleates cytoplasmic assemblysomes that support co-translational assembly of proteasome subunits (PMID:30692646). Beyond RNA turnover, CNOT1 interacts with 53BP1 and suppresses 53BP1-p53-p21 signaling (PMID:40742806).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1994 High

    Established that NOT1/CDC39 is not an isolated factor but the organizing member of a discrete multi-subunit nuclear NOT complex acting as a global transcriptional repressor.

    Evidence Yeast genetics, two-hybrid interaction and biochemical co-fractionation of NOT1-NOT4

    PMID:7926748 PMID:8428577

    Open questions at the time
    • Did not resolve whether repression was direct or via downstream RNA metabolism
    • No structural basis for subunit assembly
  2. 2000 High

    Localized the essential scaffolding function to the Not1 C-terminal domain, which is required to assemble the catalytic Ccr4 nuclease into the larger complex.

    Evidence Domain deletion, sucrose gradient co-fractionation and in vivo complementation in yeast

    PMID:11023781

    Open questions at the time
    • Atomic interfaces not defined
    • Mechanism linking assembly to enzymatic activity unresolved
  3. 2011 High

    Demonstrated that human CNOT1 is functionally required for CCR4-NOT deadenylase activity and serves as the bridge by which the ARE-binding protein TTP recruits deadenylase to target mRNAs.

    Evidence Co-IP, siRNA knockdown, deadenylase activity assays and mRNA decay readouts in human cells

    PMID:21278420 PMID:21976065

    Open questions at the time
    • Binding interface on CNOT1 not mapped
    • Whether other adaptors use the same surface unknown
  4. 2014 High

    Resolved the modular structural logic by which CNOT1 partitions deadenylation, decapping/repression and miRNA silencing through distinct domains binding CAF1, DDX6 and CNOT9.

    Evidence Crystal structures of CNOT1 MIF4G-CAF1, MIF4G-DDX6 and DUF3819-CNOT9 complexes with functional mutagenesis

    PMID:22959269 PMID:22977175 PMID:24768540 PMID:25035296

    Open questions at the time
    • How modules are coordinated on a single mRNA not shown
    • Regulation of domain occupancy unaddressed
  5. 2015 High

    Showed that CNOT1 couples deadenylation to translational repression by recruiting DDX6/Xp54 and 4E-T, with cap-dependent repression downstream of CAF1-NOT1 association.

    Evidence Crystal structure of 4E-T CHD-DDX6-CNOT1 ternary complex and tethered reporter assays in Xenopus oocytes

    PMID:26015597 PMID:26489469

    Open questions at the time
    • Quantitative contribution of repression vs decay in vivo unclear
    • Competition among DDX6 partners on endogenous mRNAs not measured
  6. 2018 High

    Reconstituted the contribution of the CNOT1 central region and CNOT9 to stimulating the nuclease module and mapped additional structural domains (MIF4G-C, N-terminal module).

    Evidence In vitro reconstitution of pentameric complex and crystallography/SAXS of MIF4G-C and N-terminal domains

    PMID:30309886 PMID:30367941

    Open questions at the time
    • MIF4G-C functional role in humans undefined
    • Mechanism of activity stimulation not fully resolved
  7. 2019 Medium

    Extended CNOT1 function beyond decay by showing it nucleates cytoplasmic assemblysomes for co-translational assembly of proteasome subunits, and refined the TTP CNOT1-binding domain requirement in vivo.

    Evidence Imaging/ribosome profiling in yeast and human cells; CNBD-deletion knock-in mice and cell-free deadenylation

    PMID:30692646 PMID:31036567

    Open questions at the time
    • Direct CNOT1 contacts within assemblysomes not mapped
    • Range of co-translationally assembled complexes unknown
  8. 2022 Medium

    Mapped the human N-terminal module architecture (CNOT1 sandwiching CNOT10-CNOT11 with a CNOT11 antenna binding GGNBP2) and identified novel transcriptional/anti-viral partners.

    Evidence Cryo-EM/crystallography and binding assays; yeast two-hybrid and HIV reporter assays for TASOR

    PMID:35013187 PMID:36586408

    Open questions at the time
    • Functional role of GGNBP2 recruitment unclear
    • TASOR-CNOT1 link rests on single-lab assays
  9. 2022 Medium

    Defined how phosphorylation of the TTP CNOT1-interaction motif by specific kinases switches CCR4-NOT recruitment on and off, linking signaling to ARE-mRNA decay.

    Evidence Phosphomimetic/phosphonull Co-IP, kinase inhibitor and mRNA decay reporter assays

    PMID:34090459 PMID:35920669

    Open questions at the time
    • Endogenous phospho-occupancy not quantified
    • Whether other adaptors are similarly regulated unknown
  10. 2021 Medium

    Placed CNOT1 in physiological circadian control by demonstrating ZFP36L1/BRF1-directed deadenylation of Per2 mRNA sets clock period.

    Evidence Conditional knockout mice, poly(A) tail assays, BRF1 knockdown and behavioral monitoring

    PMID:35510877

    Open questions at the time
    • Direct CNOT1 occupancy on Per2 mRNA not shown
    • Generality to other clock transcripts untested
  11. 2025 Medium

    Established acute, codon-optimality-linked control of global mRNA decay by CNOT1 and revealed an unexpected non-RNA role as a suppressor of 53BP1-p53-p21 signaling.

    Evidence Auxin degron RNA-seq/decay assays with BioID; high-content microscopy, Co-IP and nuclear dynamics imaging

    PMID:40742806 PMID:41161383

    Open questions at the time
    • Mechanism connecting CNOT1 to 53BP1 dynamics unresolved
    • Whether the p53 role is decay-dependent unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How CNOT1's many adaptor-binding surfaces are dynamically selected and coordinated on individual mRNAs in vivo, and whether its transcriptional and 53BP1/p53 roles are separable from its deadenylase scaffolding, remain open.
  • No integrated model of competing adaptor occupancy
  • Direct in vivo target specificity determinants undefined
  • Mechanistic basis of non-canonical nuclear roles unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0140098 catalytic activity, acting on RNA 3 GO:0140110 transcription regulator activity 3 GO:0045182 translation regulator activity 2 GO:0003723 RNA binding 1
Localization
GO:0005634 nucleus 2 GO:0005829 cytosol 2
Pathway
R-HSA-74160 Gene expression (Transcription) 3 R-HSA-8953854 Metabolism of RNA 3 R-HSA-9909396 Circadian clock 1
Partners
53BP1CNOT11CNOT7CNOT9DDX6EIF4ENIF1 (4E-T)ZFP36 (TTP)ZFP36L1
Complex memberships
CCR4-NOT complexassemblysome

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 CDC39 (yeast NOT1 ortholog) is an essential nuclear protein that negatively regulates transcription, preferentially affecting TC-dependent transcription at the HIS3 promoter; it encodes a large 2108-amino acid nuclear protein with two glutamine-rich regions. Temperature-sensitive mutant analysis, transcriptional assays, genetic complementation The EMBO journal High 8428577
1994 NOT1(CDC39), NOT2, NOT3, and NOT4 form a discrete ~500 kDa nuclear complex that acts as a global negative regulator of transcription; NOT1 and NOT2 physically associate (two-hybrid, biochemical co-fractionation) and NOT4 interacts with NOT1 and NOT3 by two-hybrid. Allele-specific suppression, two-hybrid interaction, biochemical co-fractionation Genes & development High 7926748
1990 CDC39 acts as a negative element in the yeast mating pheromone signal transduction pathway upstream of or at the level of the transducing G protein; epistasis analysis places CDC39 function between the G protein and downstream pathway components. Genetic epistasis analysis, pheromone-inducible gene expression assays Cell regulation Medium 2099190 2111445
2000 The essential function of Not1p resides in the C-terminal domain, which associates with Not5p and is required for Ccr4p to assemble into large complexes; the N-terminal domain is dispensable for viability but required for normal growth and efficient Ccr4p incorporation into the complex. Domain deletion analysis, co-fractionation on sucrose gradients, in vivo complementation with trans-expressed domains Journal of molecular biology High 11023781
1999 Not1p directly represses transcription from a TATA-less promoter in vitro; nuclear extracts from a conditional not1 mutant show increased transcription from the HIS3 TATA-less promoter upon shift to restrictive temperature. In vitro transcription assay with nuclear extracts from conditional not1 mutant Biological chemistry Medium 10661863
2011 Human NOT1 physically interacts with the C-terminal domain of tristetraprolin (TTP) through its central region, and is required for TTP to recruit the CAF1 deadenylase to AU-rich element-containing mRNAs to promote their decay. Co-immunoprecipitation, siRNA knockdown, mRNA decay assays Nucleic acids research High 21278420
2011 CNOT1 depletion in HeLa cells reduces the deadenylase activity of immunoprecipitated CNOT6L, suppresses P-body formation, and stabilizes mRNAs, demonstrating CNOT1 is required for CCR4-NOT deadenylase activity and mRNA decay in human cells. siRNA knockdown, deadenylase activity assay, flow cytometry, immunofluorescence for P-bodies Protein & cell Medium 21976065
2012 The N-terminal arm of yeast Not1 has HEAT-repeat structure with MIF4G-fold domains; a central MIF4G domain directly recognizes Caf1, which in turn binds and positions the Ccr4 nuclease; disruption of these interfaces impairs cell growth and mRNA deadenylation in vivo. X-ray crystallography, in vivo growth and mRNA decay assays, mutagenesis Molecular cell High 22959269
2012 The CAF1-binding domain of human NOT1 adopts an MIF4G fold and binds CAF1 through a pre-formed interface, leaving the CAF1 catalytic site fully accessible to RNA substrates. X-ray crystallography of NOT1 MIF4G domain alone and in complex with CAF1 Nucleic acids research High 22977175
2013 The C-terminal arm of Not1 forms a HEAT-repeat scaffold that recruits Not2 and Not5 whose Not-box domains dimerize via noncanonical Sm-like folds; the resulting ternary complex binds poly(U) RNA in vitro with a binding site at the Not5 Not box. 2.8-Å crystal structure, in vitro RNA binding assays, in vivo growth assays with disrupting mutations Nature structural & molecular biology High 24121231
2014 The CNOT1 MIF4G domain directly interacts with the C-terminal RecA domain of DDX6, structurally resembling the eIF4G–eIF4A interaction; separately, the CNOT9 subunit binds the DUF3819 domain of CNOT1 and provides tandem tryptophan-binding pockets for TNRC6/GW182 recruitment, linking miRNA target recognition to CCR4-NOT-mediated silencing. Crystal structures of CNOT1 MIF4G–DDX6 and CNOT1 DUF3819–CNOT9 complexes, functional mutagenesis in cells Molecular cell High 24768540
2014 Human DDX6 directly binds CNOT1 via a conserved subdomain, and mutations that disrupt this DDX6–CNOT1 interaction impair miRISC-mediated gene silencing, placing DDX6 as a downstream effector of CNOT1 in the miRNA pathway. Biochemical binding assays, site-directed mutagenesis, miRNA silencing reporter assays in human cells RNA (New York, N.Y.) High 25035296
2015 The CUP-homology domain of human 4E-T interacts directly with DDX6 and contacts CNOT1 MIF4G in a ternary complex; unlike Edc3 and Pat1 FDF motifs, 4E-T CHD binding to DDX6 is not displaced by CNOT1 MIF4G, revealing mutually exclusive vs. simultaneous interaction modes. 2.1-Å crystal structure of 4E-T CHD–DDX6–CNOT1 ternary complex, in vitro competition binding assays Cell reports High 26489469
2015 In Xenopus oocytes, CAF1-mediated translational repression requires its association with NOT1; NOT1 is required to recruit Xp54 (DDX6 ortholog) and 4E-T, and repression via tethered CAF1 is cap- and eIF4E-dependent but not IRES-dependent. Affinity purification–mass spectrometry, co-immunoprecipitation, tethered reporter mRNA assays, 4E-T mutant analysis RNA (New York, N.Y.) Medium 26015597
2018 The central region of CNOT1 encompassing the MIF4G and DUF3819 domains, together with CNOT9, stimulates deadenylation activity of the reconstituted pentameric CCR4-NOT nuclease module in vitro. In vitro reconstitution of pentameric complex, deadenylation activity assays comparing subcomplexes The Biochemical journal High 30309886
2018 A connector domain of NOT1 adopts a MIF4G-like fold (MIF4G-C domain); structural comparison shows key differences from the DDX6-binding MIF4G domain that explain why MIF4G-C does not bind DDX6, and the human MIF4G-C does not stably interact with other CCR4-NOT subunits. Crystal structure of thermophilic fungal MIF4G-C domain, solution scattering of human domain, binding assays Journal of structural biology Medium 30367941
2019 NOT1-containing assemblysomes in yeast are cytoplasmic particles (distinct from stress granules and P-bodies) that depend on Not1 and facilitate co-translational assembly of proteasome subunits Rpt1 and Rpt2; this mechanism is conserved in human cells. Immunofluorescence, fluorescence in situ hybridization, ribosome profiling, genetic depletion Nature structural & molecular biology Medium 30692646
2019 The conserved C-terminal CNOT1-binding domain (CNBD) of TTP is required for full TTP activity in vivo; CNBD deletion mice have a less severe inflammatory phenotype than full TTP knockout, and recombinant TTP lacking CNBD still activates deadenylation but to a lesser extent than full-length TTP. Knock-in mouse model (CNBD deletion), macrophage mRNA decay assays, cell-free deadenylation assay with recombinant proteins Molecular and cellular biology High 31036567
2022 High-resolution structural analysis reveals the human N-terminal module is composed of CNOT1 sandwiching CNOT10 and CNOT11 via two helical domains; the most conserved domain of CNOT11 protrudes as an antenna that serves as a binding platform for GGNBP2. Multiple structural approaches (cryo-EM/crystallography), biochemical binding assays Cell reports High 36586408
2022 TASOR interacts with CNOT1 (identified by yeast two-hybrid) and synergistically represses HIV-1 LTR-derived transcript accumulation; TASOR facilitates association of RNA degradation proteins with RNA Polymerase II. Yeast two-hybrid, co-immunoprecipitation, HIV reporter assays, ChIP/RIP Nature communications Medium 35013187
2022 The TTP CNOT1 Interaction Motif (CIM) cooperates with TTP tryptophan residues (that contact CNOT9) to recruit CCR4-NOT and activate mRNA degradation; CIM phosphorylation by PKCα (not MK2) disrupts CNOT1 association, while p38-MK2 activation leaves the CIM unphosphorylated and capable of CCR4-NOT recruitment. Co-immunoprecipitation with phosphomimetic/phosphonull mutants, mRNA decay reporter assays, kinase inhibitor experiments Molecular and cellular biology Medium 35920669
2021 CNOT1 regulates the mammalian circadian clock by promoting deadenylation-dependent decay of Per2 mRNA; CNOT1 is recruited to Per2 mRNA through BRF1/ZFP36L1, and CNOT1 deficiency in mice causes circadian period lengthening with extended Per2 mRNA poly(A) tail length and increased Per2 mRNA stability. Conditional knockout mice, poly(A) tail length assay, BRF1 knockdown, circadian behavioral monitoring RNA biology Medium 35510877
2023 Depletion of Not1 and Not4 oppositely affect mRNA solubility in yeast: Not1 depletion solubilizes mitochondrial mRNAs (which become insoluble upon Not4 depletion), while Not4 depletion solubilizes mRNAs with lower non-optimal codon content that are rendered insoluble by Not1; Not1 promoter association in the nucleus may set mRNA solubility. Ribosome profiling, RNA fractionation (soluble/insoluble), ChIP, depletion of Not1/Not4 by conditional systems Genome biology Medium 36803582
2025 CNOT1 interacts with 53BP1, impacts its nuclear dynamics, and acts as a suppressor of 53BP1-p53-p21 signaling; CNOT1 loss upregulates p53 target gene expression, impairs proliferation, and suppresses cytoplasmic aggregation of mutant p53, thereby restoring its nuclear localization and functionality. High-content microscopy screen, orthogonal validation, co-immunoprecipitation, siRNA knockdown, nuclear dynamics imaging Cell reports Medium 40742806
2025 Acute auxin-induced depletion of CNOT1 in human cells causes widespread increased mRNA abundance and decreased global mRNA decay, with changes correlating with codon optimality; depletion of CNOT4 has opposite effects, unexpectedly accelerating global mRNA decay, with BioID confirming CNOT4 associates with the complex in cells despite not co-purifying by standard biochemistry. Auxin-induced degron system, transcriptome-wide RNA-seq, mRNA decay assays, BioID proximity labeling The Journal of biological chemistry Medium 41161383
2006 C. elegans LET-711 (NOT1 ortholog) is required for spindle positioning in early embryos; let-711 mutants have longer, cold-stable microtubules, larger centrosomes with elevated ZYG-9 levels, and simultaneous reduction of both ZYG-9 and LET-711 rescues spindle positioning defects of both single mutants, placing LET-711 upstream of ZYG-9. RNAi/genetic loss-of-function, live imaging of spindle positioning, cold-stability assay, genetic epistasis with zyg-9 Molecular biology of the cell Medium 16971515
2018 CNOT1 knockdown in osteosarcoma cells inhibits growth and the Hedgehog signaling pathway; CNOT1 physically interacts with LMNA (lamin A) and functions as a positive regulator of LMNA; LMNA overexpression rescues the growth and Hedgehog pathway defects of CNOT1 depletion. Co-immunoprecipitation, siRNA knockdown, RNA-seq, rescue overexpression experiments, xenograft in vivo Molecular oncology Medium 28188704
2018 CNOT1 provides a scaffold platform in human pulmonary endothelial cells for recruitment of both TTP and CNOT7; CNOT1 silencing abolishes TTP-CNOT7 co-immunoprecipitation, while CNOT7 or TTP silencing does not disrupt CNOT1 interactions with the other partner, demonstrating hierarchical assembly. Co-immunoprecipitation with sequential siRNA knockdowns, immunofluorescence co-localization, mRNA stability assays Molecular medicine reports Medium 29956766
2020 ZFP36L1 directly represses translation via AU-rich elements through an interaction with CNOT1, independent of deadenylation; this mechanism differs from TTP (does not involve 4E-HP or GIGYF2 recruitment) and from miRISC (resistant to eIF4A inhibitor silvestrol, IRES-independent). In vitro translation system from mammalian cell lysates, mutant protein functional assays, pharmacological inhibition Biochimie Medium 32311426
2021 Phosphorylation of TTP Ser316 (in the C-terminal CNOT1-binding domain) by RSK1 and MK2, and dephosphorylation by PP2A, regulates TTP interaction with CNOT1; a phosphomimetic S316D mutation weakens CNOT1 interaction and TTP-mediated TNFα mRNA destabilization. Phospho-specific antibody generation, GST pull-down, RNA pull-down, CRISPR/Cas9 TTP knockout, phosphomimetic/phosphonull mutants, kinase/phosphatase inhibitor experiments Journal of inflammation (London, England) Medium 34090459
2025 HDX-MS analysis of CNOT1 residues 800-999 (HEAT-like repeat domain) reveals that E893A/Y900A and E893Q/Y900H point mutations reduce TTP peptide interaction without perturbing overall domain structure, defining these residues as critical for molecular recognition of TTP. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) of wild-type and point mutant CNOT1(800-999) with TTP peptide Biomolecules Medium 40149939

Source papers

Stage 0 corpus · 49 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. Molecular cell 237 24768540
2011 Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolin. Nucleic acids research 191 21278420
1994 NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization. Genes & development 183 7926748
2012 Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction. Molecular cell 120 22959269
2012 The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex. Nucleic acids research 113 22977175
2014 Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1. RNA (New York, N.Y.) 108 25035296
2019 Co-translational assembly of proteasome subunits in NOT1-containing assemblysomes. Nature structural & molecular biology 95 30692646
1993 CDC39, an essential nuclear protein that negatively regulates transcription and differentially affects the constitutive and inducible HIS3 promoters. The EMBO journal 94 8428577
2015 Structure of a Human 4E-T/DDX6/CNOT1 Complex Reveals the Different Interplay of DDX6-Binding Proteins with the CCR4-NOT Complex. Cell reports 90 26489469
2000 The essential function of Not1 lies within the Ccr4-Not complex. Journal of molecular biology 86 11023781
2012 Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation. Stem cells (Dayton, Ohio) 65 22367759
2011 The role of the CNOT1 subunit of the CCR4-NOT complex in mRNA deadenylation and cell viability. Protein & cell 65 21976065
2013 Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeast Ccr4-Not complex. Nature structural & molecular biology 63 24121231
2019 A Specific CNOT1 Mutation Results in a Novel Syndrome of Pancreatic Agenesis and Holoprosencephaly through Impaired Pancreatic and Neurological Development. American journal of human genetics 48 31006513
1986 Nucleotide sequence of the yeast cell division cycle start genes CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the predicted products. Nucleic acids research 46 3018676
2014 Phylogenetic distribution and evolution of the linked RNA-binding and NOT1-binding domains in the tristetraprolin family of tandem CCCH zinc finger proteins. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 39 24697206
2022 TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. Nature communications 38 35013187
1990 CDC36 and CDC39 are negative elements in the signal transduction pathway of yeast. Cell regulation 36 2099190
1990 Mutations in cell division cycle genes CDC36 and CDC39 activate the Saccharomyces cerevisiae mating pheromone response pathway. Molecular and cellular biology 35 2111445
1983 Isolation and transcriptional characterization of three genes which function at start, the controlling event of the Saccharomyces cerevisiae cell division cycle: CDC36, CDC37, and CDC39. Molecular and cellular biology 35 6346060
2019 A CCR4-NOT Transcription Complex, Subunit 1, CNOT1, Variant Associated with Holoprosencephaly. American journal of human genetics 31 31006510
1996 Differential activation of the clustered homeobox genes CNOT2 and CNOT1 during notogenesis in the chick. Developmental biology 31 8954724
2021 The Plasmodium NOT1-G paralogue is an essential regulator of sexual stage maturation and parasite transmission. PLoS biology 25 34673764
2017 CNOT1 cooperates with LMNA to aggravate osteosarcoma tumorigenesis through the Hedgehog signaling pathway. Molecular oncology 25 28188704
2015 Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo. RNA (New York, N.Y.) 25 26015597
2006 LET-711, the Caenorhabditis elegans NOT1 ortholog, is required for spindle positioning and regulation of microtubule length in embryos. Molecular biology of the cell 23 16971515
2022 The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions. Cell reports 21 36586408
2018 The central region of CNOT1 and CNOT9 stimulates deadenylation by the Ccr4-Not nuclease module. The Biochemical journal 21 30309886
2019 Importance of the Conserved Carboxyl-Terminal CNOT1 Binding Domain to Tristetraprolin Activity In Vivo. Molecular and cellular biology 19 31036567
2018 Structural and biochemical analysis of a NOT1 MIF4G-like domain of the CCR4-NOT complex. Journal of structural biology 19 30367941
2014 Exome analysis of HIV patients submitted to dendritic cells therapeutic vaccine reveals an association of CNOT1 gene with response to the treatment. Journal of the International AIDS Society 14 24433985
2023 Not1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events. Genome biology 12 36803582
2022 The Conserved CNOT1 Interaction Motif of Tristetraprolin Regulates ARE-mRNA Decay Independently of the p38 MAPK-MK2 Kinase Pathway. Molecular and cellular biology 11 35920669
1999 In vitro transcription of a TATA-less promoter: negative regulation by the Not1 protein. Biological chemistry 9 10661863
2021 The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain. Journal of inflammation (London, England) 8 34090459
2020 The CCR4-NOT complex component NOT1 regulates RNA-directed DNA methylation and transcriptional silencing by facilitating Pol IV-dependent siRNA production. The Plant journal : for cell and molecular biology 8 32412137
2020 ARE-binding protein ZFP36L1 interacts with CNOT1 to directly repress translation via a deadenylation-independent mechanism. Biochimie 7 32311426
2024 Clinical characteristics and identification of novel CNOT1 variants in three unrelated Chinese families with Vissers-Bodmer Syndrome. Heliyon 3 38434094
2023 Pathogenicity analysis and splicing rescue of a classical splice site variant (c.1343+1G>T) of CNOT1 gene associated with neurodevelopmental disorders. American journal of medical genetics. Part A 3 37507849
2023 Vissers-Bodmer syndrome caused by a novel de novo CNOT1 frameshift variant. American journal of medical genetics. Part A 3 37818768
2018 CNOT1 is involved in TTP‑mediated ICAM‑1 and IL‑8 mRNA decay. Molecular medicine reports 3 29956766
2025 Identification of CNOT1-CCR4-NOT as a suppressor of 53BP1-p53-p21 signaling. Cell reports 2 40742806
2025 Auxin-induced depletion of human CCR4-NOT subunits reveals opposing functions of CNOT1 and CNOT4 in mRNA metabolism. The Journal of biological chemistry 2 41161383
2024 CNOT1 p.Arg535Cys variant in holoprosencephaly with late onset diabetes mellitus. American journal of medical genetics. Part A 2 39149840
2021 CNOT1 regulates circadian behaviour through Per2 mRNA decay in a deadenylation-dependent manner. RNA biology 2 35510877
2025 CNOT1 contributes to small nuclear non-coding RNA maturation. Biochemical and biophysical research communications 1 41453249
2026 Modeling patient variants of Cnot1 and Cdc42bpb results in distinct forms of congenital diaphragmatic hernia in mice. bioRxiv : the preprint server for biology 0 42124667
2025 Exploring the CNOT1(800-999) HEAT Domain and Its Interactions with Tristetraprolin (TTP) as Revealed by Hydrogen/Deuterium Exchange Mass Spectrometry. Biomolecules 0 40149939
2025 [Clinical and genetic analysis of a Chinese pedigree affected with Vissers-Bodmer syndrome due to variant of CNOT1 gene and a literature review.]. Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 0 41451494

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