Affinage

CNOT1

CCR4-NOT transcription complex subunit 1 · UniProt A5YKK6

Length
2376 aa
Mass
266.9 kDa
Annotated
2026-04-28
49 papers in source corpus 28 papers cited in narrative 28 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CNOT1 is the central scaffold of the CCR4-NOT deadenylase complex, organizing its multiple functional modules to control mRNA deadenylation, translational repression, and mRNA decay across eukaryotes. Its HEAT-repeat architecture provides distinct docking surfaces: a central MIF4G domain recruits the CAF1–CCR4 catalytic nuclease module and the translational repressor DDX6 in an eIF4G–eIF4A-like manner; a DUF3819 domain anchors CNOT9, which provides tryptophan-binding pockets for TNRC6/GW182 to couple miRNA-mediated target recognition to deadenylation; and a C-terminal HEAT arm scaffolds NOT2/NOT3 and confers RNA-binding capacity, while the N-terminal module with CNOT10/CNOT11 serves as a protein–protein interaction platform (PMID:22959269, PMID:24768540, PMID:24121231, PMID:36586408). CNOT1 recruits RNA-binding proteins such as TTP/ZFP36 family members to target AU-rich element-containing mRNAs for degradation, with TTP–CNOT1 interaction regulated by phosphorylation at the TTP CNOT1-interaction motif by multiple kinases including PKCα, RSK1, and MK2 (PMID:21278420, PMID:31036567, PMID:35920669, PMID:34090459). Beyond canonical mRNA decay, CNOT1 participates in co-translational protein assembly at cytoplasmic assemblysome particles, regulates circadian period through BRF1-mediated deadenylation of Per2 mRNA, and suppresses the 53BP1–p53–p21 signaling axis (PMID:30692646, PMID:35510877, PMID:40742806).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1993 High

    Establishing that NOT1 is an essential gene encoding a nuclear protein that negatively regulates basal transcription answered the foundational question of what cellular process NOT1 controls.

    Evidence Temperature-sensitive mutant analysis and RNA measurement in S. cerevisiae

    PMID:7926748 PMID:8428577

    Open questions at the time
    • Mechanism of transcriptional repression unknown
    • Whether NOT1 functions alone or in a complex was unclear
    • No connection to mRNA turnover yet recognized
  2. 2000 High

    Domain dissection revealed that NOT1's essential function resides within the Ccr4-Not complex, with distinct N-terminal and C-terminal domains required for association with Ccr4 and Not5 respectively, establishing NOT1 as the organizing scaffold of a multi-subunit complex.

    Evidence Domain deletion, biochemical co-fractionation, and complementation in yeast

    PMID:11023781

    Open questions at the time
    • Structural basis for each domain's interactions unknown
    • Function of the complex beyond transcription not yet explored
  3. 2011 High

    Discovery that human CNOT1 links the RNA-binding protein TTP to the CAF1 deadenylase for rapid ARE-mRNA decay, and that CNOT1 depletion destabilizes other CCR4-NOT subunits and abolishes deadenylase activity, redefined CNOT1 as the essential cytoplasmic scaffold for mRNA deadenylation.

    Evidence Reciprocal Co-IP, siRNA knockdown, mRNA stability and deadenylase activity assays in human cells

    PMID:21278420 PMID:21976065

    Open questions at the time
    • Structural basis of CNOT1–CAF1 and CNOT1–TTP interactions unresolved
    • Extent of global mRNA decay dependence on CNOT1 not yet measured
  4. 2012 High

    Crystal structures of the NOT1 MIF4G domain bound to CAF1 revealed the atomic-level mechanism by which CNOT1 tethers the deadenylase catalytic module while leaving the CAF1 active site accessible, answering how the scaffold enables enzymatic activity.

    Evidence X-ray crystallography of yeast and human NOT1 MIF4G–CAF1 complexes with mutagenesis and in vivo mRNA decay assays

    PMID:22959269 PMID:22977175

    Open questions at the time
    • Structural basis for DDX6/translational repressor recruitment not yet determined
    • How other subunits integrate around this core unclear
  5. 2013 High

    Structure of the NOT1 C-terminal HEAT arm with NOT2/NOT5 revealed an RNA-binding surface and explained how the C-terminal module scaffolds regulatory subunits, completing the picture of the complex's terminal architecture.

    Evidence 2.8 Å crystal structure, RNA-binding assays, mutagenesis, and yeast growth assays

    PMID:24121231

    Open questions at the time
    • Specificity of RNA recognition unknown
    • Functional consequence of RNA binding for mRNA decay not demonstrated
  6. 2014 High

    Structures of the CNOT1 DUF3819–CNOT9 and CNOT1 MIF4G–DDX6 complexes revealed how CNOT1 simultaneously couples miRNA-mediated target recognition (via GW182/TNRC6 binding to CNOT9) and translational repression (via DDX6 in an eIF4G–eIF4A-like mode), unifying deadenylation and translational silencing on a single scaffold.

    Evidence X-ray crystallography, mutagenesis, Co-IP, tethering and miRNA reporter silencing assays in human cells

    PMID:24768540 PMID:25035296

    Open questions at the time
    • Whether DDX6 and 4E-T occupy CNOT1 simultaneously unclear
    • In vivo stoichiometry of these subcomplexes not determined
  7. 2015 High

    Structure of the 4E-T/DDX6/CNOT1 MIF4G ternary complex and functional studies in Xenopus oocytes showed that CNOT1-mediated translational repression is cap/eIF4E-dependent and that 4E-T can coexist with CNOT1 on DDX6 while excluding Edc3/Pat1, clarifying the competitive landscape of decapping versus repression.

    Evidence 2.1 Å crystal structure, competition binding assays, tethered function assays in Xenopus oocytes

    PMID:26015597 PMID:26489469

    Open questions at the time
    • How the transition from repression to decapping is regulated in vivo remains open
    • Relevance of these interactions in somatic mammalian cells not fully tested
  8. 2019 High

    Discovery of NOT1-dependent assemblysome particles that support co-translational assembly of proteasome subunits expanded CNOT1 function beyond mRNA decay to include protein complex biogenesis, answering whether CNOT1 has roles independent of deadenylation.

    Evidence Immunofluorescence, in situ hybridization, genetic depletion, and ribosome pausing analysis in yeast and human cells

    PMID:30692646

    Open questions at the time
    • Mechanism by which NOT1 organizes co-translational assembly unknown
    • Whether assemblysome function depends on deadenylase activity unresolved
    • Full inventory of mRNAs targeted to assemblysomes not determined
  9. 2019 High

    Mouse knock-in deleting the TTP CNOT1-binding domain showed a milder inflammatory phenotype than full TTP knockout, and in vitro reconstitution confirmed TTP–CNOT1 interaction quantitatively amplifies but is not absolutely required for ARE-mRNA deadenylation, defining the physiological significance of this specific scaffold–adaptor contact.

    Evidence Mouse knock-in model, in vitro deadenylation with recombinant proteins, macrophage mRNA stability assays

    PMID:31036567

    Open questions at the time
    • Redundant recruitment pathways for TTP to CCR4-NOT not fully mapped
    • Other ZFP36 family members' CNOT1-dependent contributions in vivo not dissected
  10. 2021 Medium

    Identification of phosphorylation-dependent regulation of TTP–CNOT1 interaction by multiple kinases (MK2, RSK1, PKCα) and PP2A established that the scaffold recruitment step is a regulated signaling node, not a constitutive association.

    Evidence Phospho-specific antibodies, phosphomimetic mutagenesis, Co-IP, mRNA stability assays, kinase inhibitor treatment

    PMID:34090459 PMID:35920669

    Open questions at the time
    • Structural basis of phosphorylation-dependent dissociation not determined at atomic resolution
    • In vivo kinase specificity at individual phosphosites not fully resolved
  11. 2021 Medium

    Demonstration that CNOT1 deficiency lengthens circadian period through stabilization and poly(A) tail extension of Per2 mRNA, recruited by BRF1/ZFP36L1, linked CNOT1-mediated deadenylation to a specific physiological oscillator.

    Evidence Conditional mouse knockout, circadian behavior assays, poly(A) tail measurements, siRNA knockdown

    PMID:35510877

    Open questions at the time
    • Whether additional clock mRNAs are similarly controlled by CNOT1 not surveyed
    • Independent replication of circadian phenotype not yet reported
  12. 2022 High

    Structural elucidation of the CNOT1–CNOT10–CNOT11 N-terminal module revealed the CNOT11 antenna domain as a protein–protein interaction platform with GGNBP2 as a conserved partner, completing the structural map of all major CNOT1 scaffolding regions.

    Evidence Cryo-EM/X-ray crystallography, biochemical binding assays

    PMID:36586408

    Open questions at the time
    • Functional role of the CNOT11 antenna and GGNBP2 interaction in mRNA decay unknown
    • Whether N-terminal module contributes to substrate selectivity not tested
  13. 2025 High

    Acute CNOT1 depletion via auxin-degron in human cells confirmed global mRNA decay dependence on CNOT1 in a codon-optimality-correlated manner, while discovery of CNOT1 interaction with 53BP1 and suppression of the p53–p21 axis revealed a non-canonical role in DNA damage signaling.

    Evidence AID-mediated depletion with transcriptome-wide RNA-seq and mRNA decay assays; high-content microscopy screen, Co-IP, p53 reporter assays

    PMID:40742806 PMID:41161383

    Open questions at the time
    • Whether the 53BP1 interaction is direct or mediated through an intermediary not fully resolved
    • Mechanism linking CNOT1 to suppression of mutant p53 aggregation requires structural characterization
    • In vivo relevance of the p53 axis finding in animal models not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Major open questions include: how CNOT1 coordinates its deadenylation, translational repression, co-translational assembly, and p53-regulatory functions in different cellular contexts; whether the assemblysome and deadenylase functions are mechanistically linked; and how post-translational modifications of CNOT1 itself regulate complex remodeling.
  • No full-length CNOT1 structure exists
  • Post-translational regulation of CNOT1 itself largely unexplored
  • How CNOT1 partitions among its multiple functional roles in vivo is unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 6 GO:0005198 structural molecule activity 4 GO:0098772 molecular function regulator activity 3
Localization
GO:0005829 cytosol 4 GO:0005634 nucleus 3
Pathway
R-HSA-8953854 Metabolism of RNA 6 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-392499 Metabolism of proteins 1 R-HSA-9909396 Circadian clock 1
Partners
CNOT10CNOT11CNOT2CNOT7CNOT9DDX6TP53BP1ZFP36
Complex memberships
CCR4-NOT

Evidence

Reading pass · 28 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 NOT1 (CDC39) acts as a global negative regulator of transcription in yeast; allele-specific suppression, two-hybrid interaction, and biochemical co-fractionation show NOT1 and NOT2 are nuclear proteins associated in a discrete ~500 kDa complex; NOT1 preferentially represses TATA-less (TC-element) promoters and inhibits the basal RNA polymerase II transcription machinery. Genetic epistasis (allele-specific suppression), yeast two-hybrid, biochemical co-fractionation Genes & development High 7926748
1993 CDC39/NOT1 encodes an essential nuclear protein (~2108 aa) in yeast that negatively regulates transcription; loss-of-function increases basal transcription of many genes, preferentially affecting the TC TATA element of HIS3. Temperature-sensitive mutant analysis, RNA analysis, genetic complementation The EMBO journal High 8428577
1999 Not1 directly represses transcription from a TATA-less promoter in vitro; nuclear extracts from a conditional not1 mutant show increased transcription from the HIS3 TATA-less promoter upon shift to restrictive temperature. In vitro transcription assay using conditional mutant nuclear extracts Biological chemistry Medium 10661863
2000 The essential function of Not1 lies within the Ccr4-Not complex; the C-terminal domain of Not1 is essential for viability and associates with Not5; the N-terminal domain is required for Ccr4 to associate in large complexes. Healthy growth requires physical association of both domains with Ccr4 in large complexes. Domain deletion analysis, biochemical co-fractionation, complementation assays in yeast Journal of molecular biology High 11023781
2011 Human CNOT1 associates via its central region with the C-terminal domain of tristetraprolin (TTP) in the cytoplasm, and recruits the CAF1 deadenylase through a smaller central domain; CNOT1 is required for rapid decay of ARE-containing mRNAs and acts as a platform that allows TTP to recruit the CAF1 deadenylase. Co-immunoprecipitation, siRNA knockdown, mRNA stability assays Nucleic acids research High 21278420
2011 CNOT1 depletion in HeLa cells reduces the amounts of other CCR4-NOT subunits, decreases the specific deadenylase activity of co-immunoprecipitated CNOT6L, suppresses P-body formation, and induces ER stress-mediated apoptosis through mRNA stabilization. siRNA knockdown, flow cytometry, immunoprecipitation-deadenylase assay, immunofluorescence Protein & cell High 21976065
2012 Crystal structure of the yeast Not1 N-terminal arm reveals a HEAT-repeat structure with MIF4G fold domains; a central MIF4G domain of Not1 binds Caf1, which in turn tethers Ccr4; disruption of these interactions impairs cell growth and mRNA deadenylation/decay in vivo. X-ray crystallography, biochemical reconstitution, in vivo mRNA decay assays, mutagenesis Molecular cell High 22959269
2012 Crystal structures of the human NOT1 CAF1-binding domain alone and in complex with CAF1 show that the NOT1 MIF4G domain binds CAF1 through a pre-formed interface, leaving the CAF1 catalytic site fully accessible; this domain is conserved across eukaryotes. X-ray crystallography, structural analysis Nucleic acids research High 22977175
2013 Crystal structure of the yeast Not1 C-terminal arm with Not2 and Not5 (2.8 Å) reveals Not1 as a HEAT-repeat scaffold; Not2 and Not5 wrap around Not1 with Sm-fold Not boxes that interact with a noncanonical dimerization surface; the ternary complex binds poly(U) RNA in vitro via a site at the Not5 Not box; disruption of these interactions severely impairs growth in vivo. X-ray crystallography, RNA-binding assay, mutagenesis, yeast growth assays Nature structural & molecular biology High 24121231
2014 The CNOT9 subunit attaches to the DUF3819 domain of CNOT1; the resulting CNOT1-CNOT9 complex provides W-binding pockets in CNOT9 for TNRC6/GW182 recruitment. Additionally, the CNOT1 MIF4G domain interacts with the C-terminal RecA domain of DDX6, with a crystal structure showing striking similarity to the eIF4G-eIF4A complex; these interactions physically link miRNA target recognition to translational repression, deadenylation, and decapping. X-ray crystallography, co-immunoprecipitation, mutagenesis, tethering assays Molecular cell High 24768540
2014 Human DDX6 directly binds the MIF4G domain of CNOT1; this interaction resembles eIF4G-eIF4A binding; mutations disrupting DDX6-CNOT1 interaction impair miRISC-mediated gene silencing in human cells, placing DDX6 as a downstream effector of the miRNA pathway through CNOT1. Biochemical binding assays, mutagenesis, miRNA reporter silencing assays in human cells RNA High 25035296
2015 Crystal structure (2.1 Å) of the human 4E-T CUP-homology domain (CHD)/DDX6/CNOT1 MIF4G ternary complex shows 4E-T CHD wraps around the RecA2 domain of DDX6 and contacts CNOT1; 4E-T CHD can bind DDX6 in the presence or absence of CNOT1 MIF4G, whereas Edc3 and Pat1 FDF motifs are displaced from DDX6 upon CNOT1 MIF4G binding. X-ray crystallography, in vitro binding/competition assays Cell reports High 26489469
2015 In Xenopus oocytes, CAF1 requires association with NOT1 (the major CCR4-NOT scaffold) to repress translation of a tethered reporter mRNA; NOT1 in turn recruits Xp54 (DDX6) and 4E-T; repression by tethered CAF1, NOT1, and Xp54 is cap/eIF4E-dependent, whereas a 4E-T truncation that still binds eIF4E blocks repression. Tethered function assay in Xenopus oocytes, affinity purification-mass spectrometry, co-immunoprecipitation, mutagenesis RNA High 26015597
2018 A reconstituted pentameric human Ccr4-Not sub-complex containing the CNOT1 central region (MIF4G + DUF3819 domains) plus CNOT9, BTG2, CAF1, and CCR4 shows that the CNOT1-CNOT9 components stimulate poly(A) RNA deadenylation by the nuclease module. In vitro reconstitution of sub-complexes, deadenylation assay The Biochemical journal High 30309886
2018 Crystal structure of a NOT1 MIF4G-C connector domain from Chaetomium thermophilum reveals a MIF4G-like fold; comparison with the DDX6-binding MIF4G domain identifies structural differences explaining why the MIF4G-C domain does not interact with DDX6; human MIF4G-C does not stably interact with other CCR4-NOT subunits. X-ray crystallography, solution scattering, binding assays Journal of structural biology Medium 30367941
2019 Not1-containing assemblysomes in yeast are cytoplasmic particles (distinct from stress granules and P-bodies) that support co-translational assembly of proteasome Rpt1/Rpt2 subunits; immunofluorescence and in situ hybridization show Rpt1- and Rpt2-encoding mRNAs co-localize in particles dependent on Not1; this Not1-assemblysome function is conserved from yeast to human cells. Immunofluorescence, in situ hybridization, genetic depletion, ribosome pausing analysis Nature structural & molecular biology High 30692646
2019 Deletion of the C-terminal CNOT1-binding domain (CNBD) of tristetraprolin (TTP) in mice produces a less severe inflammatory phenotype than complete TTP knockout; in cell-free assays, TTP lacking CNBD can still activate target mRNA deadenylation by purified CCR4-NOT complexes but less efficiently, demonstrating that TTP's CNBD interaction with CNOT1 quantitatively amplifies ARE-mRNA decay. Mouse knock-in model (CNBD deletion), in vitro deadenylation assay with recombinant proteins, mRNA stability assays in macrophages Molecular and cellular biology High 31036567
2022 High-resolution structural analysis of the human CNOT1-CNOT10-CNOT11 N-terminal module shows two helical domains of CNOT1 sandwich CNOT10 and CNOT11; the conserved CNOT11 antenna domain is a protein-protein interaction platform, and GGNBP2 (tumor suppressor/spermatogenic factor) is identified as a conserved binding partner of this antenna. Cryo-EM/X-ray crystallography (multiple structural approaches), biochemical binding assays Cell reports High 36586408
2022 TASOR interacts with CNOT1 (identified by yeast two-hybrid screen), and TASOR and CNOT1 synergistically repress HIV-1 LTR expression at a post-transcriptional level; TASOR facilitates association of RNA degradation proteins with RNA Polymerase II. Yeast two-hybrid, co-immunoprecipitation, gene expression assays, ChIP Nature communications Medium 35013187
2022 The TTP CNOT1 Interaction Motif (CIM) recruits the CCR4-NOT deadenylase complex and promotes mRNA degradation cooperatively with TTP tryptophan residues that contact CNOT9; the CIM is phosphorylated by PKCα (not MK2) to disrupt CNOT1 interaction, revealing that multiple kinase pathways cooperatively regulate TTP-CNOT1 interaction. Mutagenesis of TTP CIM, co-immunoprecipitation, mRNA stability assays, phosphorylation mapping Molecular and cellular biology Medium 35920669
2021 CNOT1 is required for normal circadian period; Cnot1 deficiency in mice lengthens circadian period; Per2 mRNA has a longer poly(A) tail and increased stability in Cnot1-deficient mice; CNOT1 is recruited to Per2 mRNA through BRF1 (ZFP36L1), which oscillates in antiphase with Per2 mRNA, linking CCR4-NOT deadenylation to circadian clock control. Conditional mouse knockout, circadian behavior assays, poly(A) tail length measurement, mRNA stability assay, Co-immunoprecipitation, siRNA knockdown RNA biology Medium 35510877
2020 ZFP36L1 (BRF1) directly represses translation via ARE elements in a deadenylation-independent manner requiring direct interaction with CNOT1; this mechanism is distinct from TTP- or miRISC-mediated repression and does not require 4E-HP or GIGYF2. In vitro translation assay (mammalian cell-free system), mutagenesis of ZFP36L1-CNOT1 interaction interface, reporter assays Biochimie Medium 32311426
2023 In yeast, depletion of Not1 and Not4 have opposing effects on mRNA solubility and ribosome codon-dwelling dynamics; Not1 depletion solubilizes mitochondrial mRNAs and affects codon-optimality-dependent ribosome pausing for soluble mRNAs, suggesting Not1 inversely regulates mRNA condensate association and co-translational events relative to Not4. Ribosome profiling, RNA fractionation (soluble/insoluble), genetic depletion, transcriptomics Genome biology Medium 36803582
2025 CNOT1 interacts with 53BP1 (identified by high-content microscopy screens and orthogonal validation), impacts 53BP1 nuclear dynamics, and suppresses the 53BP1-p53-p21 signaling axis; CNOT1 depletion upregulates p53 target gene expression, induces apoptosis, and suppresses cytoplasmic aggregation of mutant p53, restoring nuclear localization and functionality of p53 mutants. High-content microscopy screen, Co-immunoprecipitation, CNOT1 depletion (siRNA/genetic), p53 reporter assays, FRAP/nuclear dynamics analysis Cell reports Medium 40742806
2025 Auxin-induced acute depletion of CNOT1 in human cells causes decreased global mRNA decay and increased abundance of thousands of transcripts in a codon-optimality-correlated manner; CNOT4 depletion has opposite effects (accelerated mRNA decay) and does not disrupt CCR4-NOT complex integrity. Auxin-induced degron (AID) system, transcriptome-wide RNA-seq, mRNA decay assays, BioID proximity labeling The Journal of biological chemistry High 41161383
2006 LET-711, the C. elegans NOT1 ortholog, is required for spindle positioning in early embryos; partial loss-of-function causes defects in centration and rotation of the first mitotic spindle; let-711 mutants have longer, more cold-stable microtubules and larger centrosomes with elevated ZYG-9 (XMAP215 homolog); simultaneous reduction of ZYG-9 and LET-711 rescues spindle positioning defects, placing LET-711 as an inhibitor of ZYG-9 expression for normal centrosome size and microtubule dynamics. RNAi/mutant analysis in C. elegans, live imaging, genetic epistasis (double mutant rescue), centrosome measurement Molecular biology of the cell High 16971515
2021 The TTP Ser316 residue located at the C-terminal NOT1-binding domain is phosphorylated by RSK1 and MK2, and dephosphorylated by PP2A; a phosphomimetic S316D mutation weakens TTP interaction with CNOT1, dissociating TTP from the CCR4-NOT deadenylase complex and reducing TTP-mediated mRNA destabilization. Phospho-specific antibody, GST pulldown, Co-immunoprecipitation, mRNA stability assay, phosphomimetic mutagenesis, kinase inhibitor treatment Journal of inflammation Medium 34090459
2025 HDX-MS analysis of CNOT1(800-999) HEAT-like repeat domain shows that point mutations E893A/Y900A and E893Q/Y900H reduce binding to TTP peptide by removing critical contact residues, not by perturbing overall domain structure, defining the molecular basis of TTP recognition by this CNOT1 domain. Hydrogen/deuterium exchange mass spectrometry (HDX-MS), mutagenesis, binding assays Biomolecules Medium 40149939

Source papers

Stage 0 corpus · 49 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. Molecular cell 236 24768540
2011 Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolin. Nucleic acids research 191 21278420
1994 NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization. Genes & development 183 7926748
2012 Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction. Molecular cell 120 22959269
2012 The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex. Nucleic acids research 113 22977175
2014 Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1. RNA (New York, N.Y.) 108 25035296
1993 CDC39, an essential nuclear protein that negatively regulates transcription and differentially affects the constitutive and inducible HIS3 promoters. The EMBO journal 94 8428577
2019 Co-translational assembly of proteasome subunits in NOT1-containing assemblysomes. Nature structural & molecular biology 92 30692646
2015 Structure of a Human 4E-T/DDX6/CNOT1 Complex Reveals the Different Interplay of DDX6-Binding Proteins with the CCR4-NOT Complex. Cell reports 90 26489469
2000 The essential function of Not1 lies within the Ccr4-Not complex. Journal of molecular biology 86 11023781
2012 Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation. Stem cells (Dayton, Ohio) 65 22367759
2011 The role of the CNOT1 subunit of the CCR4-NOT complex in mRNA deadenylation and cell viability. Protein & cell 64 21976065
2013 Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeast Ccr4-Not complex. Nature structural & molecular biology 63 24121231
2019 A Specific CNOT1 Mutation Results in a Novel Syndrome of Pancreatic Agenesis and Holoprosencephaly through Impaired Pancreatic and Neurological Development. American journal of human genetics 46 31006513
1986 Nucleotide sequence of the yeast cell division cycle start genes CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the predicted products. Nucleic acids research 46 3018676
2022 TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. Nature communications 38 35013187
2014 Phylogenetic distribution and evolution of the linked RNA-binding and NOT1-binding domains in the tristetraprolin family of tandem CCCH zinc finger proteins. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 38 24697206
1990 CDC36 and CDC39 are negative elements in the signal transduction pathway of yeast. Cell regulation 36 2099190
1990 Mutations in cell division cycle genes CDC36 and CDC39 activate the Saccharomyces cerevisiae mating pheromone response pathway. Molecular and cellular biology 35 2111445
1983 Isolation and transcriptional characterization of three genes which function at start, the controlling event of the Saccharomyces cerevisiae cell division cycle: CDC36, CDC37, and CDC39. Molecular and cellular biology 35 6346060
1996 Differential activation of the clustered homeobox genes CNOT2 and CNOT1 during notogenesis in the chick. Developmental biology 31 8954724
2019 A CCR4-NOT Transcription Complex, Subunit 1, CNOT1, Variant Associated with Holoprosencephaly. American journal of human genetics 30 31006510
2015 Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo. RNA (New York, N.Y.) 25 26015597
2017 CNOT1 cooperates with LMNA to aggravate osteosarcoma tumorigenesis through the Hedgehog signaling pathway. Molecular oncology 24 28188704
2021 The Plasmodium NOT1-G paralogue is an essential regulator of sexual stage maturation and parasite transmission. PLoS biology 23 34673764
2006 LET-711, the Caenorhabditis elegans NOT1 ortholog, is required for spindle positioning and regulation of microtubule length in embryos. Molecular biology of the cell 23 16971515
2018 The central region of CNOT1 and CNOT9 stimulates deadenylation by the Ccr4-Not nuclease module. The Biochemical journal 21 30309886
2022 The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions. Cell reports 20 36586408
2019 Importance of the Conserved Carboxyl-Terminal CNOT1 Binding Domain to Tristetraprolin Activity In Vivo. Molecular and cellular biology 19 31036567
2018 Structural and biochemical analysis of a NOT1 MIF4G-like domain of the CCR4-NOT complex. Journal of structural biology 19 30367941
2020 The thick aleurone1 Gene Encodes a NOT1 Subunit of the CCR4-NOT Complex and Regulates Cell Patterning in Endosperm. Plant physiology 18 32737073
2014 Exome analysis of HIV patients submitted to dendritic cells therapeutic vaccine reveals an association of CNOT1 gene with response to the treatment. Journal of the International AIDS Society 14 24433985
2023 Not1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events. Genome biology 12 36803582
2022 The Conserved CNOT1 Interaction Motif of Tristetraprolin Regulates ARE-mRNA Decay Independently of the p38 MAPK-MK2 Kinase Pathway. Molecular and cellular biology 11 35920669
1999 In vitro transcription of a TATA-less promoter: negative regulation by the Not1 protein. Biological chemistry 9 10661863
2021 The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain. Journal of inflammation (London, England) 8 34090459
2020 The CCR4-NOT complex component NOT1 regulates RNA-directed DNA methylation and transcriptional silencing by facilitating Pol IV-dependent siRNA production. The Plant journal : for cell and molecular biology 8 32412137
2020 ARE-binding protein ZFP36L1 interacts with CNOT1 to directly repress translation via a deadenylation-independent mechanism. Biochimie 7 32311426
2024 Clinical characteristics and identification of novel CNOT1 variants in three unrelated Chinese families with Vissers-Bodmer Syndrome. Heliyon 3 38434094
2023 Vissers-Bodmer syndrome caused by a novel de novo CNOT1 frameshift variant. American journal of medical genetics. Part A 3 37818768
2018 CNOT1 is involved in TTP‑mediated ICAM‑1 and IL‑8 mRNA decay. Molecular medicine reports 3 29956766
2025 Auxin-induced depletion of human CCR4-NOT subunits reveals opposing functions of CNOT1 and CNOT4 in mRNA metabolism. The Journal of biological chemistry 2 41161383
2024 CNOT1 p.Arg535Cys variant in holoprosencephaly with late onset diabetes mellitus. American journal of medical genetics. Part A 2 39149840
2023 Pathogenicity analysis and splicing rescue of a classical splice site variant (c.1343+1G>T) of CNOT1 gene associated with neurodevelopmental disorders. American journal of medical genetics. Part A 2 37507849
2021 CNOT1 regulates circadian behaviour through Per2 mRNA decay in a deadenylation-dependent manner. RNA biology 2 35510877
2025 Identification of CNOT1-CCR4-NOT as a suppressor of 53BP1-p53-p21 signaling. Cell reports 1 40742806
2025 CNOT1 contributes to small nuclear non-coding RNA maturation. Biochemical and biophysical research communications 1 41453249
2025 Exploring the CNOT1(800-999) HEAT Domain and Its Interactions with Tristetraprolin (TTP) as Revealed by Hydrogen/Deuterium Exchange Mass Spectrometry. Biomolecules 0 40149939
2025 [Clinical and genetic analysis of a Chinese pedigree affected with Vissers-Bodmer syndrome due to variant of CNOT1 gene and a literature review.]. Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 0 41451494