Affinage

USP32

Ubiquitin carboxyl-terminal hydrolase 32 · UniProt Q8NFA0

Round 2 corrected
Length
1604 aa
Mass
181.7 kDa
Annotated
2026-04-28
130 papers in source corpus 11 papers cited in narrative 11 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

USP32 is a broadly expressed, Golgi-localized deubiquitinating enzyme that controls endosomal and lysosomal trafficking by reversibly removing ubiquitin from small GTPases and lysosomal signaling components. It deubiquitylates Rab7 to regulate the balance between late-endosome transport and retromer-mediated recycling (PMID:30926795), deubiquitylates Rab35 (K48-linked chains) to promote exosome secretion (PMID:36725886), and removes non-proteolytic ubiquitin from the Ragulator subunit LAMTOR1 to sustain vacuolar H⁺-ATPase interaction and mTORC1 recruitment at lysosomes (PMID:36476874). USP32 also stabilizes oncogenic substrates including SLC35F2 (via ER-associated degradation) (PMID:34815782), BAG3 (activating the RAF–MEK–ERK cascade) (PMID:39030175), and SMAD2 (PMID:32226309), linking its deubiquitinase activity to cancer cell proliferation, migration, and drug resistance across multiple tumor types.

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 2003 Medium

    Establishing the evolutionary identity of USP32 as an ancient, broadly expressed DUB that served as the ancestral template for the hominoid-specific oncogene USP6 (Tre2) resolved the gene's origin and indicated functional conservation across vertebrates.

    Evidence Phylogenetic and genomic analysis of USP32/TBC1D3/USP6 loci with expression profiling

    PMID:12604796

    Open questions at the time
    • No biochemical DUB activity demonstrated at this point
    • Endogenous substrates unknown
  2. 2010 Medium

    Demonstrating that USP32 possesses conserved catalytic residues, localizes to the Golgi apparatus, and is required for normal cell proliferation and migration established its enzymatic competence and subcellular context.

    Evidence GFP-fusion fluorescence protection assay with BODIPY-TR Golgi costaining, shRNA knockdown with proliferation/migration assays

    PMID:20549504

    Open questions at the time
    • In vitro DUB activity not directly measured
    • No substrates identified
    • Golgi localization not confirmed by endogenous staining
  3. 2017 Medium

    Showing that USP32 silencing arrests cells in G0/G1 (via CDK4/Cyclin D1 loss and p21 induction), activates apoptosis, and suppresses EMT markers in SCLC cells expanded the proliferative phenotype to a defined cell-cycle and apoptotic mechanism.

    Evidence siRNA knockdown with flow cytometry, Western blotting for cell-cycle regulators and EMT markers, invasion assays in SCLC lines

    PMID:28597490

    Open questions at the time
    • Direct DUB substrates mediating cell-cycle effects unidentified
    • Single cancer type examined
  4. 2019 High

    Identification of Rab7 as a direct USP32 substrate via SILAC ubiquitome profiling, and demonstration that USP32-dependent deubiquitylation toggles Rab7 between RILP-mediated transport and retromer-mediated recycling, provided the first mechanistic link between USP32 and endosomal trafficking.

    Evidence SILAC ubiquitome profiling in USP32-KO hTERT-RPE1 cells, live-cell imaging, cargo recycling assays, co-IP with RILP and retromer subunits

    PMID:30926795

    Open questions at the time
    • Ubiquitin chain type on Rab7 not fully defined
    • Upstream signals regulating USP32-Rab7 axis unknown
    • Impact on in vivo endosomal trafficking not tested
  5. 2019 Medium

    Validation that miR-let-7a directly targets the USP32 3′-UTR to suppress its expression, with USP32 re-expression rescuing proliferation, established a post-transcriptional regulatory input controlling USP32 abundance.

    Evidence Dual-luciferase 3′-UTR reporter assay, miRNA mimic/inhibitor transfection, rescue experiments in MCF-7 cells

    PMID:35116927

    Open questions at the time
    • Physiological contexts where let-7a–USP32 axis operates are uncharacterized
    • Other miRNAs regulating USP32 not surveyed
  6. 2020 Medium

    Demonstration that USP32 stabilizes SMAD2 in gastric cancer, with SMAD2 re-expression rescuing proliferation/migration upon USP32 loss, expanded the substrate repertoire to TGF-β signaling components.

    Evidence shRNA knockdown with SMAD2 rescue, in vivo xenograft, IHC

    PMID:32226309

    Open questions at the time
    • Direct deubiquitylation of SMAD2 not shown with in vitro DUB assay
    • Ubiquitin chain type on SMAD2 undetermined
  7. 2021 High

    An unbiased CRISPR screen identified USP32 as the principal determinant of SLC35F2 protein stability, revealing that USP32 paradoxically promotes ER-associated degradation of SLC35F2 to confer drug resistance, uncovering a non-canonical substrate relationship.

    Evidence Genome-scale CRISPR KO screen, USP32 KO validation, co-IP, ubiquitination assays, in vivo xenograft

    PMID:34815782

    Open questions at the time
    • Mechanism by which a DUB promotes ERAD of SLC35F2 is not fully resolved
    • Whether USP32 acts on an intermediate rather than SLC35F2 directly is unclear
  8. 2022 High

    Discovery that USP32 deubiquitylates LAMTOR1 to maintain Ragulator–v-ATPase interaction and mTORC1 lysosomal recruitment, confirmed cross-species by CYK-3 depletion in C. elegans, established USP32 as a gatekeeper of nutrient-sensing signaling.

    Evidence USP32 CRISPR-KO, ubiquitome proteomics, mTORC1 activity assays (S6K/4E-BP1 phosphorylation), v-ATPase co-IP, C. elegans CYK-3 depletion

    PMID:36476874

    Open questions at the time
    • Ubiquitin chain type on LAMTOR1 not specified
    • Whether USP32 acts at lysosomes or Golgi for LAMTOR1 deubiquitylation is unresolved
  9. 2023 High

    Identification of Rab35 as a USP32 substrate (K48-linked ubiquitin removal) that links USP32 to exosome-mediated transmission of imatinib resistance in GISTs extended the DUB's trafficking role to the exosome biogenesis pathway and revealed ETV1 as a transcriptional activator of USP32.

    Evidence K48-linkage-specific ubiquitination assays, Rab35 KD/OE, exosome quantification, ETV1 ChIP and reporter assays in GIST cells

    PMID:36725886

    Open questions at the time
    • Whether USP32–Rab35 axis operates outside the GIST context is untested
    • Other chain types on Rab35 not examined
  10. 2024 Medium

    Identification of BAG3 as a direct USP32 substrate that activates RAF–MEK–ERK signaling in NSCLC established a new oncogenic axis and demonstrated substrate-level epistasis through BAG3 rescue experiments.

    Evidence AP-MS, co-IP, ubiquitination assays, shRNA KD with BAG3 rescue, RAF/MEK/ERK phosphorylation Western blots, in vivo xenograft

    PMID:39030175

    Open questions at the time
    • Ubiquitin chain type on BAG3 not determined
    • Whether BAG3 stabilization connects to the mTORC1/autophagy axis is unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • A unified structural and regulatory model explaining how USP32 selects among its diverse substrates (Rab7, Rab35, LAMTOR1, BAG3, SMAD2, SLC35F2), whether substrate access is determined by Golgi/endosome localization or by adaptor proteins, and how USP32's own catalytic activity is regulated post-translationally, remains to be established.
  • No crystal structure or cryo-EM structure available
  • No post-translational regulatory mechanism for USP32 activity defined
  • Substrate selectivity determinants unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 6 GO:0016787 hydrolase activity 5
Localization
GO:0005764 lysosome 1 GO:0005768 endosome 1 GO:0005794 Golgi apparatus 1
Pathway
R-HSA-392499 Metabolism of proteins 5 R-HSA-162582 Signal Transduction 2 R-HSA-5653656 Vesicle-mediated transport 2 R-HSA-9612973 Autophagy 1
Partners
BAG3LAMTOR1RAB35RAB7ASLC35F2SMAD2

Evidence

Reading pass · 11 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2010 USP32 encodes an active deubiquitinating enzyme with conserved catalytic USP-domain residues (asparagine, cysteine, histidine, aspartic acid) and localizes to the Golgi apparatus, as demonstrated by GFP-fusion fluorescence protection assay and BODIPY-TR staining. Stable silencing of USP32 significantly decreases cell proliferation and migration rates. GFP-fusion fluorescence protection assay, BODIPY-TR Golgi staining, stable shRNA knockdown with proliferation and migration assays Mammalian Genome Medium 20549504
2003 USP32 (also known as NY-REN-60) is an ancient, highly conserved deubiquitinase gene that served as the ancestral template for the hominoid-specific oncogene Tre2 (USP6), which arose through chimeric fusion of USP32 with TBC1D3. USP32 is broadly expressed across tissues, in contrast to the testis-specific Tre2. Phylogenetic analysis, genomic sequencing, expression profiling Proceedings of the National Academy of Sciences Medium 12604796
2009 Global proteomic analysis of deubiquitinase interaction networks identified USP32 as a DUB with candidate interacting proteins, placing it within the broader DUB interaction landscape. Affinity purification–mass spectrometry (AP-MS) with CompPASS bioinformatics Cell Low 19615732
2017 Silencing of USP32 in small cell lung cancer (SCLC) cells inhibits proliferation and migration, arrests the cell cycle at G0/G1 phase by decreasing CDK4/Cyclin D1 complex and elevating p21, induces apoptosis via activation of cleaved caspase-3 and cleaved PARP, and reduces cell invasiveness by altering EMT marker expression. siRNA knockdown, cell viability assay, cell cycle analysis by flow cytometry, Western blotting for CDK4/Cyclin D1/p21/caspase-3/PARP, invasion assay Cell Proliferation Medium 28597490
2019 USP32 deubiquitylates Rab7, the small GTPase central to late endosome (LE) biology. Loss of USP32 inhibits late endosome transport and recycling of LE cargos, resulting in dispersion and swelling of the late endosomal compartment. SILAC-based ubiquitome profiling identified Rab7 as a USP32 substrate. The LE transport effector RILP preferentially binds ubiquitylation-deficient Rab7, while retromer-mediated LE recycling requires an intact cycle of Rab7 ubiquitylation, suggesting USP32-dependent reversible ubiquitylation switches Rab7 between its transport and recycling functions. SILAC-based ubiquitome profiling, USP32 knockout/knockdown, live-cell imaging, cargo recycling assays, Co-IP with RILP and retromer Nature Communications High 30926795
2020 USP32 knockdown or depletion significantly inhibits gastric cancer (GC) cell proliferation and migration in vitro and in vivo. Mechanistically, USP32 silencing causes decreased expression of SMAD2, and re-introduction of SMAD2 rescues the inhibitory effects, indicating USP32 promotes GC progression through stabilization of SMAD2. siRNA/shRNA knockdown, in vivo xenograft, immunohistochemistry, Western blotting, SMAD2 rescue experiments International Journal of Biological Sciences Medium 32226309
2021 CRISPR-based dual screening identified USP32 as a deubiquitinase that destabilizes SLC35F2 (a solute-carrier protein essential for uptake of the anticancer compound YM155), thereby conferring YM155 resistance. USP32 and SLC35F2 expression are negatively correlated; targeted USP32 knockout verified it as the main determinant of SLC35F2 protein stability in vitro and in vivo. USP32 promotes ER-associated degradation of SLC35F2. Genome-scale CRISPR-Cas9 knockout screen, Western blotting, in vivo xenograft, co-immunoprecipitation, ubiquitination assays Theranostics High 34815782
2022 USP32 knockout in hTERT-RPE1 cells results in hyperubiquitination of LAMTOR1, a Ragulator complex subunit. Accumulation of LAMTOR1 ubiquitination impairs its interaction with the vacuolar H+-ATPase, reduces Ragulator function, limits mTORC1 recruitment to lysosomes, decreases mTORC1 activity, and induces autophagy. Depletion of the USP32 homolog CYK-3 in C. elegans similarly results in mTOR inhibition and autophagy induction. USP32 knockout (CRISPR), ubiquitome proteomics, mTOR localization by immunofluorescence, mTORC1 activity assays (S6K/4E-BP1 phosphorylation), v-ATPase interaction Co-IP, C. elegans CYK-3 depletion Cell Reports High 36476874
2023 USP32 deubiquitylates Rab35 by removing K48-linked ubiquitin chains, protecting it from proteasomal degradation. Elevated Rab35 in imatinib-resistant GISTs increases exosome secretion that transmits drug resistance to sensitive tumors. The transcription factor ETV1 promotes USP32 expression in GISTs. Co-immunoprecipitation, ubiquitination assays (K48-linkage specific), Rab35 knockdown/overexpression, exosome secretion quantification, iTRAQ proteomics, ETV1 ChIP/reporter assays Oncogene High 36725886
2019 miR-let-7a directly targets the 3'-UTR of USP32 mRNA to suppress its protein expression, and overexpression of USP32 reverses let-7a-mediated inhibition of MCF-7 breast cancer cell proliferation, placing USP32 downstream of let-7a in a proliferation-regulatory axis. miRNA mimic/inhibitor transfection, dual-luciferase reporter assay (3'-UTR), Western blotting, MTT proliferation assay, colony formation Translational Cancer Research Medium 35116927
2024 USP32 interacts with BAG3 and deubiquitinates and stabilizes BAG3 protein in a deubiquitinating activity-dependent manner in non-small cell lung cancer (NSCLC) cells. USP32-mediated BAG3 stabilization increases phosphorylation of the RAF/MEK/ERK signaling pathway. Restoration of BAG3 abrogates antitumor effects of USP32 silencing, establishing USP32→BAG3→RAF/MEK/ERK as an oncogenic axis in NSCLC. AP-MS to identify interacting proteins, Co-IP, ubiquitination assays, shRNA knockdown, BAG3 rescue experiments, Western blotting for RAF/MEK/ERK phosphorylation, in vivo xenograft Oncogenesis Medium 39030175

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 2861 17081983
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2009 Defining the human deubiquitinating enzyme interaction landscape. Cell 1282 19615732
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13. Cell 689 21962518
2003 Human and mouse proteases: a comparative genomic approach. Nature reviews. Genetics 680 12838346
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2010 USP10 regulates p53 localization and stability by deubiquitinating p53. Cell 510 20096447
2016 G3BP-Caprin1-USP10 complexes mediate stress granule condensation and associate with 40S subunits. The Journal of cell biology 507 27022092
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2010 Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics. Cell 318 21145461
2018 An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Nature communications 201 29568061
2020 USP10 Promotes Proliferation of Hepatocellular Carcinoma by Deubiquitinating and Stabilizing YAP/TAZ. Cancer research 158 32217697
2001 Ras-GAP SH3 domain binding protein (G3BP) is a modulator of USP10, a novel human ubiquitin specific protease. Oncogene 158 11439350
2018 Discovery and Characterization of ZUFSP/ZUP1, a Distinct Deubiquitinase Class Important for Genome Stability. Molecular cell 150 29576527
1999 Antigens recognized by autologous antibody in patients with renal-cell carcinoma. International journal of cancer 150 10508479
2017 RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination. BMC biology 135 29117863
2016 Deubiquitination and Activation of AMPK by USP10. Molecular cell 130 26876938
2019 The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2. Molecular cell 124 30639242
2003 The Tre2 (USP6) oncogene is a hominoid-specific gene. Proceedings of the National Academy of Sciences of the United States of America 123 12604796
2017 Inhibition of USP10 induces degradation of oncogenic FLT3. Nature chemical biology 120 28967922
2017 An Interaction Landscape of Ubiquitin Signaling. Molecular cell 119 28190767
2019 The deubiquitinase USP10 regulates KLF4 stability and suppresses lung tumorigenesis. Cell death and differentiation 116 31748695
2020 The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation. Molecular cell 109 31981475
2010 Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Molecular medicine (Cambridge, Mass.) 108 20379614
2009 The deubiquitinating enzyme USP10 regulates the post-endocytic sorting of cystic fibrosis transmembrane conductance regulator in airway epithelial cells. The Journal of biological chemistry 100 19398555
2022 EZH2 depletion potentiates MYC degradation inhibiting neuroblastoma and small cell carcinoma tumor formation. Nature communications 99 35013218
2018 Ubiquitin-Specific Peptidase 10 (USP10) Inhibits Hepatic Steatosis, Insulin Resistance, and Inflammation Through Sirt6. Hepatology (Baltimore, Md.) 98 29698567
2022 Hypoxia-induced circWSB1 promotes breast cancer progression through destabilizing p53 by interacting with USP10. Molecular cancer 95 35351136
2015 TRAF Family Member-associated NF-κB Activator (TANK) Inhibits Genotoxic Nuclear Factor κB Activation by Facilitating Deubiquitinase USP10-dependent Deubiquitination of TRAF6 Ligase. The Journal of biological chemistry 91 25861989
2021 NLRP7 deubiquitination by USP10 promotes tumor progression and tumor-associated macrophage polarization in colorectal cancer. Journal of experimental & clinical cancer research : CR 87 33838681
2020 Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains. Cell reports 79 32814053
2018 Association of USP10 with G3BP2 Inhibits p53 Signaling and Contributes to Poor Outcome in Prostate Cancer. Molecular cancer research : MCR 79 29378906
2013 HTLV-1 Tax oncoprotein stimulates ROS production and apoptosis in T cells by interacting with USP10. Blood 78 23775713
2020 When the chains do not break: the role of USP10 in physiology and pathology. Cell death & disease 77 33277473
2018 Oncogene-induced senescence mediated by c-Myc requires USP10 dependent deubiquitination and stabilization of p14ARF. Cell death and differentiation 73 29472714
2019 Deubiquitinating enzyme USP10 promotes hepatocellular carcinoma metastasis through deubiquitinating and stabilizing Smad4 protein. Molecular oncology 72 31721429
2017 USP10 inhibits lung cancer cell growth and invasion by upregulating PTEN. Molecular and cellular biochemistry 69 28852924
2023 Stabilization of IGF2BP1 by USP10 promotes breast cancer metastasis via CPT1A in an m6A-dependent manner. International journal of biological sciences 66 36632454
2019 USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7. Nature communications 65 30926795
2005 The ubiquitin-specific protease USP10 modulates androgen receptor function. Molecular and cellular endocrinology 63 16368182
2021 The deubiquitinase USP10 restores PTEN activity and inhibits non-small cell lung cancer cell proliferation. The Journal of biological chemistry 62 34416231
2018 USP10 suppresses tumor progression by inhibiting mTOR activation in hepatocellular carcinoma. Cancer letters 61 30056112
2014 The palmitoyl acyltransferase HIP14 shares a high proportion of interactors with huntingtin: implications for a role in the pathogenesis of Huntington's disease. Human molecular genetics 58 24705354
2022 HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway. Journal of experimental & clinical cancer research : CR 55 35277183
2020 Suppression of the USP10/CCND1 axis induces glioblastoma cell apoptosis. Acta pharmacologica Sinica 55 33184448
2023 INKILN is a Novel Long Noncoding RNA Promoting Vascular Smooth Muscle Inflammation via Scaffolding MKL1 and USP10. Circulation 54 37199168
2022 Structure and hepatoprotective activity of Usp10/NF-κB/Nrf2 pathway-related Morchella esculenta polysaccharide. Carbohydrate polymers 54 36657860
2020 The USP10-HDAC6 axis confers cisplatin resistance in non-small cell lung cancer lacking wild-type p53. Cell death & disease 53 32382008
2010 USP32 is an active, membrane-bound ubiquitin protease overexpressed in breast cancers. Mammalian genome : official journal of the International Mammalian Genome Society 52 20549504
2022 Physical and functional interactome atlas of human receptor tyrosine kinases. EMBO reports 50 35384245
2024 Modulation of YBX1-mediated PANoptosis inhibition by PPM1B and USP10 confers chemoresistance to oxaliplatin in gastric cancer. Cancer letters 47 38364962
2022 USP10 as a Potential Therapeutic Target in Human Cancers. Genes 47 35627217
2020 RIG-I regulates myeloid differentiation by promoting TRIM25-mediated ISGylation. Proceedings of the National Academy of Sciences of the United States of America 47 32513696
2016 RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation. Nature communications 47 27558965
2022 NUDT21 limits CD19 levels through alternative mRNA polyadenylation in B cell acute lymphoblastic leukemia. Nature immunology 46 36138187
2017 Generation and Validation of Intracellular Ubiquitin Variant Inhibitors for USP7 and USP10. Journal of molecular biology 46 28587923
2022 Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma. Cell death and differentiation 45 36526897
2016 Ubiquitin-specific Peptidase 10 (USP10) Deubiquitinates and Stabilizes MutS Homolog 2 (MSH2) to Regulate Cellular Sensitivity to DNA Damage. The Journal of biological chemistry 44 26975374
2014 MicroRNA-191 promotes pancreatic cancer progression by targeting USP10. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 44 25168367
2019 TRAF4 Promotes Fibroblast Proliferation in Keloids by Destabilizing p53 via Interacting with the Deubiquitinase USP10. The Journal of investigative dermatology 43 30940456
2018 USP10 Is a Driver of Ubiquitinated Protein Aggregation and Aggresome Formation to Inhibit Apoptosis. iScience 43 30469013
2021 BioID reveals an ATG9A interaction with ATG13-ATG101 in the degradation of p62/SQSTM1-ubiquitin clusters. EMBO reports 42 34369648
2012 Direct and indirect control of mitogen-activated protein kinase pathway-associated components, BRAP/IMP E3 ubiquitin ligase and CRAF/RAF1 kinase, by the deubiquitylating enzyme USP15. The Journal of biological chemistry 42 23105109
2020 Potent USP10/13 antagonist spautin-1 suppresses melanoma growth via ROS-mediated DNA damage and exhibits synergy with cisplatin. Journal of cellular and molecular medicine 41 32129945
2019 Long noncoding RNA Mirt2 upregulates USP10 expression to suppress hepatic steatosis by sponging miR-34a-5p. Gene 41 30898698
2021 USP10 alleviates sepsis-induced acute kidney injury by regulating Sirt6-mediated Nrf2/ARE signaling pathway. Journal of inflammation (London, England) 40 34412625
2020 Wu-5, a novel USP10 inhibitor, enhances crenolanib-induced FLT3-ITD-positive AML cell death via inhibiting FLT3 and AMPK pathways. Acta pharmacologica Sinica 39 32694757
2015 Protein interactome mining defines melatonin MT1 receptors as integral component of presynaptic protein complexes of neurons. Journal of pineal research 39 26514267
2014 Deubiquitination and stabilization of T-bet by USP10. Biochemical and biophysical research communications 39 24845384
2022 Melatonin inhibits ESCC tumor growth by mitigating the HDAC7/β-catenin/c-Myc positive feedback loop and suppressing the USP10-maintained HDAC7 protein stability. Military Medical Research 37 36163081
2018 USP10 regulates the stability of the EMT-transcription factor Slug/SNAI2. Biochemical and biophysical research communications 36 29803676
2010 The deubiquitinating enzyme USP10 regulates the endocytic recycling of CFTR in airway epithelial cells. Channels (Austin, Tex.) 36 20215869
2023 A microprotein N1DARP encoded by LINC00261 promotes Notch1 intracellular domain (N1ICD) degradation via disrupting USP10-N1ICD interaction to inhibit chemoresistance in Notch1-hyperactivated pancreatic cancer. Cell discovery 34 37714834
2019 USP10 regulates Musashi-2 stability via deubiquitination and promotes tumour proliferation in colon cancer. FEBS letters 34 30604502
2023 USP10 deubiquitinates RUNX1 and promotes proneural-to-mesenchymal transition in glioblastoma. Cell death & disease 33 36949071
2020 USP32 promotes tumorigenesis and chemoresistance in gastric carcinoma via upregulation of SMAD2. International journal of biological sciences 33 32226309
2023 LINC00240 in the 6p22.1 risk locus promotes gastric cancer progression through USP10-mediated DDX21 stabilization. Journal of experimental & clinical cancer research : CR 31 37072811
2017 Downregulation of USP32 inhibits cell proliferation, migration and invasion in human small cell lung cancer. Cell proliferation 30 28597490
2020 Inhibition of the deubiquitinase USP10 induces degradation of SYK. British journal of cancer 28 32015510
2019 USP10 protects against cerebral ischemia injury by suppressing inflammation and apoptosis through the inhibition of TAK1 signaling. Biochemical and biophysical research communications 28 31301769
2019 G3BP1 inhibits ubiquitinated protein aggregations induced by p62 and USP10. Scientific reports 28 31501480
2021 The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity. The Journal of biological chemistry 27 33577797
2023 The AEG-1-USP10-PARP1 axis confers radioresistance in esophageal squamous cell carcinoma via facilitating homologous recombination-dependent DNA damage repair. Cancer letters 26 37838281
2021 Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84. Bioengineered 26 34753388
2023 Deubiquitinating PABPC1 by USP10 upregulates CLK2 translation to promote tumor progression in pancreatic ductal adenocarcinoma. Cancer letters 25 37757903
2017 The deubiquitylase USP10 regulates integrin β1 and β5 and fibrotic wound healing. Journal of cell science 25 28851806
2010 USP10: friend and foe. Cell 25 20144755
2023 KIF15 is essential for USP10-mediated PGK1 deubiquitination during the glycolysis of pancreatic cancer. Cell death & disease 24 36807568
2023 USP10 Regulates ZEB1 Ubiquitination and Protein Stability to Inhibit ZEB1-Mediated Colorectal Cancer Metastasis. Molecular cancer research : MCR 24 36940483
2022 Limonin stabilises sirtuin 6 (SIRT6) by activating ubiquitin specific peptidase 10 (USP10) in cardiac hypertrophy. British journal of pharmacology 24 35727596
2023 USP32 deubiquitinase: cellular functions, regulatory mechanisms, and potential as a cancer therapy target. Cell death discovery 23 37679322
2021 USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2. Theranostics 23 34815782
2022 USP10 regulates B cell response to SARS-CoV-2 or HIV-1 nanoparticle vaccines through deubiquitinating AID. Signal transduction and targeted therapy 22 34983926
2022 USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction. Cell reports 22 36476874
2024 Stabilization of MOF (KAT8) by USP10 promotes esophageal squamous cell carcinoma proliferation and metastasis through epigenetic activation of ANXA2/Wnt signaling. Oncogene 21 38317006
2023 USP10 is a potential mediator for vagus nerve stimulation to alleviate neuroinflammation in ischaemic stroke by inhibiting NF-κB signalling pathway. Frontiers in immunology 20 37153558
2021 LncRNA GASAL1 promotes hepatocellular carcinoma progression by up-regulating USP10-stabilized PCNA. Experimental cell research 20 34914965
2023 Deubiquitylation of Rab35 by USP32 promotes the transmission of imatinib resistance by enhancing exosome secretion in gastrointestinal stromal tumours. Oncogene 19 36725886
2021 FoxO4 negatively modulates USP10 transcription to aggravate the apoptosis and oxidative stress of hypoxia/reoxygenation-induced cardiomyocytes by regulating the Hippo/YAP pathway. Journal of bioenergetics and biomembranes 19 34251583
2020 USP10 deletion inhibits macrophage-derived foam cell formation and cellular-oxidized low density lipoprotein uptake by promoting the degradation of CD36. Aging 19 33197885
2024 DCAF7 Acts as A Scaffold to Recruit USP10 for G3BP1 Deubiquitylation and Facilitates Chemoresistance and Metastasis in Nasopharyngeal Carcinoma. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 18 38973296
2023 USP10 strikes down β-catenin by dual-wielding deubiquitinase activity and phase separation potential. Cell chemical biology 18 37611590
2022 USP10 Inhibits Aberrant Cytoplasmic Aggregation of TDP-43 by Promoting Stress Granule Clearance. Molecular and cellular biology 18 35007165
2025 USP10/XAB2/ANXA2 axis promotes DNA damage repair to enhance chemoresistance to oxaliplatin in colorectal cancer. Journal of experimental & clinical cancer research : CR 17 40069750
2023 USP10 Contributes to Colon Carcinogenesis via mTOR/S6K Mediated HIF-1α but Not HIF-2α Protein Synthesis. Cells 17 37371055
2023 Downregulated circPOKE promotes breast cancer metastasis through activation of the USP10-Snail axis. Oncogene 17 37717099
2019 RNAi Screening-based Identification of USP10 as a Novel Regulator of Paraptosis. Scientific reports 17 30894572
2024 The USP10/13 inhibitor, spautin-1, attenuates the progression of glioblastoma by independently regulating RAF-ERK mediated glycolysis and SKP2. Biochimica et biophysica acta. Molecular basis of disease 16 38857836
2024 FOXP4-AS1 promotes CD8+ T cell exhaustion and esophageal cancer immune escape through USP10-stabilized PD-L1. Immunologic research 15 38687433
2024 USP32 facilitates non-small cell lung cancer progression via deubiquitinating BAG3 and activating RAF-MEK-ERK signaling pathway. Oncogenesis 15 39030175
2024 NDR1 mediates PD-L1 deubiquitination to promote prostate cancer immune escape via USP10. Cell communication and signaling : CCS 15 39227807
2021 USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells. The Journal of biological chemistry 15 34599966
2024 Tuning Immune-Cold Tumor by Suppressing USP10/B7-H4 Proteolytic Axis Reinvigorates Therapeutic Efficacy of ADCs. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 14 39206932
2024 Recruitment of USP10 by GCS1 to deubiquitinate GRP78 promotes the progression of colorectal cancer via alleviating endoplasmic reticulum stress. Journal of experimental & clinical cancer research : CR 14 39267084
2023 USP10 regulates macrophage inflammation responses via stabilizing NEMO in LPS-induced sepsis. Inflammation research : official journal of the European Histamine Research Society ... [et al.] 14 37436447
2024 Stress-induced epinephrine promotes hepatocellular carcinoma progression via the USP10-PLAGL2 signaling loop. Experimental & molecular medicine 13 38689092
2024 Deubiquitinase USP10 promotes osteosarcoma autophagy and progression through regulating GSK3β-ULK1 axis. Cell & bioscience 13 39218913
2022 USP10 Alleviates Palmitic Acid-induced Steatosis through Autophagy in HepG2 Cells. Journal of clinical and translational hepatology 13 36406315
2023 The deubiquitinase USP10 mediates crosstalk between the LKB1/AMPK axis and Wnt/β-catenin signaling in cancer. FEBS letters 12 37873736
2022 Loss of Ras GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) inhibits the progression of ovarian cancer in coordination with ubiquitin-specific protease 10 (USP10). Bioengineered 12 34967276
2019 MicroRNA let-7a inhibits proliferation of breast cancer cell by downregulating USP32 expression. Translational cancer research 12 35116927
2025 AGD1/USP10/METTL13 complexes enhance cancer stem cells proliferation and diminish the therapeutic effect of docetaxel via CD44 m6A modification in castration resistant prostate cancer. Journal of experimental & clinical cancer research : CR 11 39806412
2024 USP10 drives cancer stemness and enables super-competitor signalling in colorectal cancer. Oncogene 11 39443725
2022 The deubiquitinase USP10 protects pancreatic cancer cells from endoplasmic reticulum stress. NPJ precision oncology 11 36543867