Affinage

DRG1

Protein NDRG1 · UniProt Q92597

Length
394 aa
Mass
42.8 kDa
Annotated
2026-06-09
33 papers in source corpus 19 papers cited in narrative 19 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

DRG1 is a conserved nucleotide-binding protein that has been characterized in two principal mechanistic contexts: AAA-ATPase-driven ribosome maturation in yeast and GTPase/microtubule-associated functions in mammalian cells (PMID:17646390, PMID:28855639). In yeast, the AAA-ATPase Drg1 is essential for cytoplasmic maturation of the pre-60S ribosomal subunit, where it releases the shuttling adaptor Rlp24 from exported pre-60S particles; Rlp24 itself recruits Drg1 and stimulates its ATPase activity, hydrolysis in the D2 domain drives substrate release, and subsequent D1 hydrolysis dissociates Drg1 to reset the cycle, with nucleoporin Nup116 promoting the reaction (PMID:17646390, PMID:23185031). Cryo-EM structures show Drg1 hexamers cycling between planar and helical states with pore-loops forming a spiral staircase that engages polypeptide sequence-independently, defining Drg1 as an unfoldase that threads substrate through its central pore; the inhibitor diazaborine forms covalent ATP adducts in the ATPase domains to lock a non-productive conformation (PMID:24371142, PMID:36351914). In mammalian cells DRG1 is a potassium-dependent GTPase whose stability and catalytic activity are governed by its binding partner DFRP1, which blocks DRG1 poly-ubiquitination and proteolysis and stimulates GTPase activity ~4-fold (PMID:15676025, PMID:23711155). DRG1 also acts as a microtubule-associated protein that diffuses on microtubules, promotes their polymerization, bundling and stabilization, localizes to centrosomes, and supports spindle formation and the p53-dependent spindle checkpoint independently of GTP hydrolysis (PMID:15247272, PMID:28855639). At the cellular level, DRG1 functions as a metastasis suppressor by transcriptionally downregulating ATF3, maintains epithelial junction integrity through RAC1 regulation, and its mRNA stability is controlled by METTL3/ELAVL1-dependent m6A modification (PMID:17178897, PMID:33471252, PMID:32266933). Biallelic loss-of-function DRG1 variants that impair GTPase activity and disrupt binding to the DFRP1 ortholog ZC3H15 cause a recessive neurodevelopmental disorder, and Drg1 knockout is lethal in mice, establishing the protein as essential for mammalian development (PMID:37179472).

Mechanistic history

Synthesis pass · year-by-year structured walk · 19 steps
  1. 2007 High

    Established that Drg1 ATPase activity is essential for a defined step of ribosome biogenesis, answering what cellular process this AAA-ATPase serves.

    Evidence Genetic inactivation and dominant-negative D2 domain mutant with subcellular fractionation in S. cerevisiae

    PMID:17646390

    Open questions at the time
    • Did not identify the direct substrate released
    • Mechanism of substrate engagement unknown
  2. 2012 High

    Resolved how Drg1 acts on pre-60S particles by identifying Rlp24 as the specific extracted substrate and dissecting the ordered D2-then-D1 hydrolysis cycle, answering the directionality and recruitment logic.

    Evidence In vitro reconstitution with purified pre-60S particles, ATPase assays, and domain mutagenesis

    PMID:23185031

    Open questions at the time
    • Structural basis of substrate threading not yet resolved
    • Role of Nup116 interaction not structurally defined
  3. 2013 High

    Identified diazaborine's mechanism of action as ATP-dependent binding into the D2 domain, validating Drg1 as a druggable target and confirming D2 hydrolysis as the critical step.

    Evidence Drug-target identification, biochemical inhibition assays, and ATPase domain mutagenesis in yeast

    PMID:24371142

    Open questions at the time
    • Structural detail of the inhibited state addressed only later
    • Selectivity over other AAA-ATPases not fully defined
  4. 2017 Medium

    Showed the N-domain regulates ATPase activity via inter-domain communication with D1, answering how Drg1's catalytic cycle is internally coordinated.

    Evidence Mutagenesis of N-domain/D1 interface with ATPase and Rlp24-binding assays in yeast

    PMID:28303975

    Open questions at the time
    • Limited structural validation in this study
    • Physiological consequences of dysregulation not tested in vivo
  5. 2022 High

    Provided the structural mechanism: cryo-EM revealed planar-to-helical hexamer transitions and a substrate-engaged spiral pore-loop staircase, establishing Drg1 as a polypeptide-threading unfoldase and explaining diazaborine inhibition.

    Evidence Cryo-EM in multiple nucleotide and inhibitor states with structure-based mutagenesis

    PMID:36351914

    Open questions at the time
    • Identity of the threaded substrate region within pre-60S not defined
    • Coupling to downstream maturation factors unresolved
  6. 2025 Medium

    Connected the yeast Drg1 paradigm to humans by showing the metazoan AAA-ATPase SPATA5 evolved from Drg1 and performs cytoplasmic pre-60S maturation within the 55LCC complex.

    Evidence Interaction screen, cryo-EM, crystallography, and acute depletion with ATPase mutant swap-in (preprint)

    PMID:bio_10.1101_2025.11.07.687187

    Open questions at the time
    • DRG1 itself studied as evolutionary ancestor rather than directly
    • Preprint, not peer-reviewed
    • Whether human DRG1 retains any pre-60S role unaddressed
  7. 2005 High

    Identified DFRP1 as the specific stabilizing partner of mammalian DRG1, answering how DRG1 protein levels are controlled.

    Evidence Co-immunoprecipitation, dfrp1 knockout cell lines, and immunofluorescence

    PMID:15676025

    Open questions at the time
    • Identity of the ubiquitin ligase acting on DRG1 unknown
    • Functional output of stabilization not yet defined
  8. 2009 Medium

    Showed the DRG1/DFRP1 complex co-sediments with polysomes whereas DRG2/DFRP2 does not, distinguishing DRG1 as the translation-associated paralog.

    Evidence Polysome fractionation and co-sedimentation in mammalian cells

    PMID:19819225

    Open questions at the time
    • Direct role in translation not functionally demonstrated
    • Single lab
  9. 2013 High

    Defined DRG1 as a potassium-dependent GTPase and pinpointed the DFRP domain as the 4-fold catalytic stimulator, establishing the enzymatic regulation of the mammalian protein.

    Evidence Purified recombinant GTPase assays with domain deletion and point mutants

    PMID:23711155

    Open questions at the time
    • Physiological GTPase substrate/effector unknown
    • Cellular consequence of GTP hydrolysis not addressed here
  10. 2004 Medium

    First linked DRG1 to mitotic fidelity, showing it is a centrosome- and microtubule-associated protein required for spindle integrity in a p53-dependent checkpoint.

    Evidence Immunofluorescence, siRNA knockdown, ectopic overexpression, and flow cytometry with spindle inhibitors

    PMID:15247272

    Open questions at the time
    • Molecular basis of p53 dependence unresolved
    • Single lab
  11. 2017 High

    Reconstituted DRG1's direct microtubule activities, showing it diffuses on, polymerizes, bundles and stabilizes microtubules independently of GTP hydrolysis, separating this function from its GTPase cycle.

    Evidence In vitro polymerization and TIRF assays, siRNA in HeLa, live imaging, and domain truncations

    PMID:28855639

    Open questions at the time
    • How non-catalytic microtubule activity integrates with GTPase function unclear
    • Single lab
  12. 2004 Medium

    Placed DRG1 downstream of PTEN/Akt signaling, showing PTEN transcriptionally upregulates DRG1, linking it to a tumor-suppressor pathway.

    Evidence PTEN overexpression/knockdown and Akt inhibitor treatment in prostate and breast cancer cells

    PMID:15520163

    Open questions at the time
    • Transcription factor mediating PTEN-dependent induction unidentified
    • Single lab
  13. 2006 Medium

    Identified ATF3 as a downstream effector, establishing transcriptional ATF3 repression as a mechanism of DRG1 metastasis suppression.

    Evidence Microarray, siRNA, ATF3 promoter luciferase assay, and in vivo SCID metastasis model

    PMID:17178897

    Open questions at the time
    • Mechanism by which DRG1 represses the ATF3 promoter unknown
    • Single lab
  14. 2006 Medium

    Identified a cytoplasmic DRG1–NADE interaction and a proliferation-promoting role antagonized by NADE, expanding DRG1's interactome.

    Evidence Co-IP, in vitro binding, domain mapping, and cell-cycle flow cytometry

    PMID:16777077

    Open questions at the time
    • Mechanistic link between binding and proliferation unresolved
    • Single lab, limited depth
  15. 2020 Medium

    Established post-transcriptional control of DRG1, showing METTL3-deposited m6A and ELAVL1 binding stabilize DRG1 mRNA.

    Evidence m6A measurement, METTL3/ELAVL1 knockdown, and mRNA stability assays in osteosarcoma cells

    PMID:32266933

    Open questions at the time
    • Functional output of DRG1 in osteosarcoma not mechanistically tied to m6A
    • Single lab
  16. 2021 Medium

    Defined a DRG1 role in epithelial barrier maintenance through RAC1 regulation, broadening its physiological function.

    Evidence Interactome MS, DRG1 silencing/overexpression, permeability assays, RAC1 inhibitor, and in vivo NEC model

    PMID:33471252

    Open questions at the time
    • Direct biochemical link between DRG1 and RAC1 activation not established
    • Single lab
  17. 2022 Medium

    Demonstrated evolutionary conservation of DRG1's monomeric state, DFRP1 complex formation, nucleic acid binding, and tubulin influence from sponge to human.

    Evidence Recombinant protein purification, binding assays, immunofluorescence, and α-tubulin dynamics assay

    PMID:35790840

    Open questions at the time
    • Biological significance of nonspecific nucleic acid binding unclear
    • Single lab
  18. 2023 High

    Established DRG1 as a Mendelian disease gene, showing biallelic loss-of-function variants impair GTPase activity and ZC3H15/DFRP1 binding and cause a recessive neurodevelopmental disorder, with mouse knockout lethal.

    Evidence Patient fibroblast analysis, GTPase assays on mutants, Co-IP binding, and mouse Drg1 knockout

    PMID:37179472

    Open questions at the time
    • Cellular pathway whose disruption causes neurodevelopmental phenotype unresolved
    • Tissue-specific requirements not defined
  19. 2025 Low

    Linked DRG1 to cellular senescence via a NO2/ROS-induced interaction with CDK5 in bronchial epithelium.

    Evidence Co-IP, siRNA, NAC treatment, β-galactosidase and cell-cycle assays

    PMID:42070819

    Open questions at the time
    • Single Co-IP for DRG1-CDK5 without reciprocal validation
    • Indirect mechanistic placement
    • Direct functional consequence of the interaction undefined

Open questions

Synthesis pass · forward-looking unresolved questions
  • Whether mammalian DRG1 retains a direct role in cytoplasmic pre-60S ribosome maturation analogous to its yeast ortholog, and how its GTPase, microtubule, and ribosome-associated activities are integrated, remains unresolved.
  • No human GTPase effector/substrate identified
  • Relationship between DRG1 and the metazoan SPATA5/55LCC pre-60S machinery undefined
  • Mechanism connecting molecular activities to the neurodevelopmental phenotype unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 3 GO:0140657 ATP-dependent activity 3 GO:0003924 GTPase activity 2 GO:0008092 cytoskeletal protein binding 2 GO:0140110 transcription regulator activity 1
Localization
GO:0005829 cytosol 2 GO:0005856 cytoskeleton 2 GO:0005815 microtubule organizing center 1 GO:0005840 ribosome 1
Pathway
R-HSA-8953854 Metabolism of RNA 3 R-HSA-1640170 Cell Cycle 2 R-HSA-1266738 Developmental Biology 1
Partners
CDK5DFRP1NADENUP116RAC1RLP24ZC3H15
Complex memberships
DRG1–DFRP1 complex

Evidence

Reading pass · 19 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2007 The AAA-ATPase Drg1 is essential for 60S ribosomal subunit maturation in yeast; functional inactivation of Drg1 causes cytoplasmic accumulation of shuttling pre-60S maturation factors (Rlp24, Arx1, Tif6, Nog1), and dominant-negative mutation in the D2 ATPase domain recapitulates this phenotype, demonstrating that ATPase activity is required for release of shuttling proteins from pre-60S particles shortly after nuclear export. Genetic inactivation, dominant-negative D2 ATPase domain mutant, subcellular fractionation, fluorescence microscopy in S. cerevisiae Molecular and cellular biology High 17646390
2012 The shuttling protein Rlp24 recruits Drg1 to pre-60S particles and stimulates its ATPase activity; ATP hydrolysis in the second AAA domain (D2) of Drg1 is required to release shuttling proteins. In vitro, Drg1 specifically and exclusively extracts Rlp24 from purified pre-60S particles in an ATP-dependent manner promoted by nucleoporin Nup116 interaction. Subsequent ATP hydrolysis in the first AAA domain (D1) dissociates Drg1 from Rlp24, enabling consecutive activity cycles. In vitro reconstitution with purified pre-60S particles, ATPase assays, domain mutagenesis, biochemical fractionation The Journal of cell biology High 23185031
2013 Diazaborine inhibits ribosome biogenesis by targeting the AAA-ATPase Drg1; the drug binds into the second AAA domain (D2) of Drg1 in an ATP-dependent manner, inhibiting ATP hydrolysis at D2, thereby blocking Rlp24 release from pre-60S particles and preventing cytoplasmic preribosome maturation. Drug-target identification, biochemical inhibition assays, ATPase domain mutagenesis in yeast The Journal of biological chemistry High 24371142
2022 Cryo-EM structures of yeast Drg1 in different nucleotide-binding states and benzo-diazaborine-treated states reveal that Drg1 hexamers transition between planar and helical conformations; benzo-diazaborine forms covalent adducts with ATP in both ATPase domains, locking the hexamer in a symmetric non-productive conformation that inhibits inter-protomer and inter-ring communication. A substrate-engaged mutant structure shows conserved pore-loops forming a spiral staircase interacting with polypeptide in a sequence-independent manner, suggesting Drg1 functions as an unfoldase that threads a substrate protein within the pre-60S particle. Cryo-EM structure determination, structure-based mutagenesis, biochemical inhibition assays Nature communications High 36351914
2005 DFRP1 (DRG family regulatory protein 1) specifically binds DRG1 and stabilizes it by blocking poly-ubiquitination that would otherwise lead to proteolysis; the DRG1–DFRP1 interaction occurs in the cytoplasm. Knockout of dfrp1 reduces endogenous DRG1 expression, confirming DFRP1 is a specific in vivo regulator of DRG1 stability. Co-immunoprecipitation, transient transfection, dfrp1 knockout cell lines, immunofluorescence Genes to cells : devoted to molecular & cellular mechanisms High 15676025
2009 The DRG1/DFRP1 complex co-sediments with polysomes in mammalian cells, whereas the DRG2/DFRP2 complex does not associate with ribosomal fractions, indicating that DRG1/DFRP1 specifically modulates protein synthesis mechanisms distinct from DRG2/DFRP2. Polysome fractionation, co-sedimentation analysis, biochemical characterization in mammalian cells Biochemical and biophysical research communications Medium 19819225
2013 Human DRG1 is a potassium-dependent GTPase; potassium strongly stimulates GTPase activity without changing DRG1 monomeric status. The DFRP domain of Lerepo4 (DFRP1) is solely responsible for a 4-fold stimulation of DRG1 catalytic activity and increased thermal stability, without affecting nucleotide affinity, possibly by favoring switch I reorientation via the TGS domain. Purified recombinant protein GTPase assays, domain deletion/point mutants, biochemical characterization The FEBS journal High 23711155
2004 DRG1/Rit42 is a microtubule-associated protein that localizes to centrosomes and participates in the spindle checkpoint in a p53-dependent manner; ectopic expression inhibits polyploidy in p53-deficient tumor cells and increases mitotic arrest upon spindle inhibitor treatment. siRNA knockdown of endogenous Rit42 in normal mammary epithelial cells causes loss of astral microtubules, failure of spindle fiber formation, and microtubule inhibitor-induced reduplication leading to polyploidy. Immunofluorescence localization, siRNA knockdown, ectopic overexpression, flow cytometry, spindle inhibitor treatment The Journal of biological chemistry Medium 15247272
2017 Human DRG1 is a microtubule-binding protein that can diffuse on microtubules, promote their polymerization, drive microtubule bundling, and stabilize microtubules in vitro. In HeLa cells, reduced DRG1 levels delay progression from prophase to anaphase due to slowed spindle formation. GTP hydrolysis by DRG1 is not required for these microtubule-associated functions, but all protein domains are required for activities beyond microtubule binding. In vitro microtubule polymerization assays, TIRF microscopy, siRNA knockdown in HeLa cells, live-cell imaging, domain truncation analysis Scientific reports High 28855639
2017 The N-terminal domain of Drg1 regulates ATPase activity through inter-domain communication with the adjacent D1 AAA domain; mutations at the N-domain/D1 interface dysregulate ATPase activity and alter interaction with the substrate Rlp24, resembling pathological mutations in the related AAA-ATPase p97 that cause IBMPFD. Mutagenesis of N-domain/D1 interface, ATPase activity assays, binding assays with Rlp24 in yeast Scientific reports Medium 28303975
2004 PTEN up-regulates DRG1 expression through an Akt-dependent pathway at the transcriptional level; overexpression of PTEN augments endogenous DRG1 protein, while siRNA knockdown of PTEN decreases DRG1, and a phospho-Akt inhibitor restores DRG1 expression in prostate and breast cancer cells. PTEN overexpression and siRNA knockdown, Akt inhibitor treatment, protein expression analysis, promoter/transcription level assessment Cancer research Medium 15520163
2006 DRG-1 suppresses the expression of ATF3 (activating transcription factor 3) at the transcriptional level in prostate cancer cells; DRG-1 induction suppresses ATF3 mRNA/protein, DRG-1 siRNA up-regulates ATF3, and DRG-1 suppresses ATF3 promoter activity. ATF3 overexpression promotes invasiveness and enhances spontaneous lung metastasis in SCID mice, identifying ATF3 downregulation as a downstream mechanism of DRG-1 metastasis suppression. Microarray analysis, siRNA knockdown, ATF3 promoter luciferase assay, overexpression, in vivo SCID mouse metastasis model Cancer research Medium 17178897
2006 DRG-1 interacts with human NADE (p75NTR-associated cell death executor) in vivo and in vitro; the interaction occurs in the cytoplasm and requires the N-terminal of DRG-1 and the C-terminal of NADE. Stable DRG-1 expression promotes cell proliferation (increased S phase population) in 293 and PC12 cells, and this promotion is suppressed by NADE overexpression. Co-immunoprecipitation, in vitro binding, immunofluorescence co-localization, domain mapping, flow cytometry cell cycle analysis Brain research Medium 16777077
2021 DRG1 maintains intestinal epithelial cell junctions and barrier function by regulating RAC1 activity; DRG1 deficiency destabilizes E-cadherin and occludin at the cell membrane and increases epithelial monolayer permeability, while DRG1 overexpression prevents LPS-induced disruption. RAC1 inhibition with NSC23766 attenuates intestinal injury consistent with DRG1 acting upstream of RAC1. Immunoprecipitation/mass spectrometry interactome, stable DRG1 silencing/overexpression in Caco2 and FHs74Int cells, permeability assays, RAC1 inhibitor treatment, in vivo NEC model Digestive diseases and sciences Medium 33471252
2023 Loss-of-function DRG1 germline variants (stop-gained and missense) cause a recessive neurodevelopmental disorder; patient-derived fibroblasts show severely disrupted DRG1 mRNA/protein stability, impaired GTPase activity of the mutant protein, and compromised binding to partner protein ZC3H15 (DFRP1 ortholog). Targeted inactivation of mouse Drg1 results in preweaning lethality, establishing that DRG1 is essential for mammalian development. Patient-derived fibroblast analysis, in vitro GTPase activity assays on mutant proteins, co-immunoprecipitation binding assay, mouse Drg1 knockout Genetics in medicine High 37179472
2020 METTL3-mediated m6A modification of DRG1 mRNA promotes its stability via ELAVL1 (HuR) binding; knockdown of METTL3 decreases m6A levels and DRG1 mRNA, while ELAVL1 knockdown impairs DRG1 mRNA stability, reducing both mRNA and protein levels in osteosarcoma cells. m6A measurement, siRNA knockdown of METTL3 and ELAVL1, mRNA stability assays, qPCR and western blot in osteosarcoma cells Bioscience reports Medium 32266933
2025 NO2/NaNO3 promotes the interaction between DRG1 and CDK5 during cellular senescence, as demonstrated by co-immunoprecipitation. ROS-dependent upregulation of DRG1 and CDK5 mediates NO2-induced bronchial epithelial senescence, and si-DRG1 treatment alleviates senescence phenotypes including G1 arrest and senescence marker expression. Co-immunoprecipitation, siRNA knockdown, ROS inhibitor (NAC) treatment, β-galactosidase activity assay, cell cycle analysis Journal of environmental sciences (China) Low 42070819
2022 Both recombinant sponge and human DRG1 are predominantly monomers that form complexes with DFRP1 and bind non-specifically to RNA and DNA; DRG1 influences α-tubulin dynamics and its intracellular localization is cytosolic, conserved between sponge and human. Recombinant protein purification, biochemical binding assays, immunofluorescence localization, α-tubulin dynamics assay Scientific reports Medium 35790840
2025 SPATA5 (a metazoan-specific AAA-ATPase) evolved from the yeast Drg1 ortholog and operates within the eumetazoan-specific SPATA5-SPATA5L1-CINP-C1ORF109 (55LCC) complex for cytoplasmic pre-60S maturation in human cells; acute depletion of each component impairs pre-60S maturation, and SPATA5 ATPase activity is functionally more important than SPATA5L1's. Protein-protein interaction screen, cryo-EM, X-ray crystallography, acute depletion, ATPase mutant swap-in bioRxivpreprint Medium bio_10.1101_2025.11.07.687187

Source papers

Stage 0 corpus · 33 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Drg-1 as a differentiation-related, putative metastatic suppressor gene in human colon cancer. Cancer research 285 10676663
2003 The Drg-1 gene suppresses tumor metastasis in prostate cancer. Cancer research 271 12702552
2004 Role of the putative tumor metastasis suppressor gene Drg-1 in breast cancer progression. Oncogene 162 15184886
1999 Differential expression of the RTP/Drg1/Ndr1 gene product in proliferating and growth arrested cells. Biochimica et biophysica acta 131 10395947
2004 PTEN up-regulates the tumor metastasis suppressor gene Drg-1 in prostate and breast cancer. Cancer research 115 15520163
1999 Identification of new genes ndr2 and ndr3 which are related to Ndr1/RTP/Drg1 but show distinct tissue specificity and response to N-myc. Biochemical and biophysical research communications 108 10581191
2006 The tumor metastasis suppressor gene Drg-1 down-regulates the expression of activating transcription factor 3 in prostate cancer. Cancer research 101 17178897
2007 Cytoplasmic recycling of 60S preribosomal factors depends on the AAA protein Drg1. Molecular and cellular biology 90 17646390
1999 The differentiation-related gene 1, Drg1, is markedly upregulated by androgens in LNCaP prostatic adenocarcinoma cells. FEBS letters 86 10428464
2012 Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation. The Journal of cell biology 61 23185031
2005 Drg1 expression in 131 colorectal liver metastases: correlation with clinical variables and patient outcomes. Clinical cancer research : an official journal of the American Association for Cancer Research 57 15867226
2002 Drg1, a novel target for modulating sensitivity to CPT-11 in colon cancer cells. Cancer research 54 12124325
2013 The drug diazaborine blocks ribosome biogenesis by inhibiting the AAA-ATPase Drg1. The Journal of biological chemistry 53 24371142
2004 Function of Drg1/Rit42 in p53-dependent mitotic spindle checkpoint. The Journal of biological chemistry 53 15247272
2005 Identification of DRG family regulatory proteins (DFRPs): specific regulation of DRG1 and DRG2. Genes to cells : devoted to molecular & cellular mechanisms 50 15676025
2020 m6A-dependent up-regulation of DRG1 by METTL3 and ELAVL1 promotes growth, migration, and colony formation in osteosarcoma. Bioscience reports 49 32266933
2001 Regulation of the differentiation-related gene Drg-1 during mouse skin carcinogenesis. Molecular carcinogenesis 36 11746822
2017 Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics. Scientific reports 24 28855639
2009 Independent stabilizations of polysomal Drg1/Dfrp1 complex and non-polysomal Drg2/Dfrp2 complex in mammalian cells. Biochemical and biophysical research communications 24 19819225
2016 DRG1 is a potential oncogene in lung adenocarcinoma and promotes tumor progression via spindle checkpoint signaling regulation. Oncotarget 19 27626498
2013 Human Drg1 is a potassium-dependent GTPase enhanced by Lerepo4. The FEBS journal 15 23711155
2023 A Glu209Lys substitution in DRG1/TaACT7, which disturbs F-actin organization, reduces plant height and grain length in bread wheat. The New phytologist 11 37668262
2022 Structural dynamics of AAA + ATPase Drg1 and mechanism of benzo-diazaborine inhibition. Nature communications 10 36351914
2015 Identification of DRG-1 As a Melanoma-Associated Antigen Recognized by CD4+ Th1 Cells. PloS one 9 25993655
2021 DRG1 Maintains Intestinal Epithelial Cell Junctions and Barrier Function by Regulating RAC1 Activity in Necrotizing Enterocolitis. Digestive diseases and sciences 7 33471252
2006 Characterization of human dopamine responsive protein DRG-1 that binds to p75NTR-associated cell death executor NADE. Brain research 7 16777077
2022 Structure and function of cancer-related developmentally regulated GTP-binding protein 1 (DRG1) is conserved between sponges and humans. Scientific reports 6 35790840
2017 A conserved inter-domain communication mechanism regulates the ATPase activity of the AAA-protein Drg1. Scientific reports 5 28303975
2023 Transfection of Sponge Cells and Intracellular Localization of Cancer-Related MYC, RRAS2, and DRG1 Proteins. Marine drugs 4 36827160
2012 Association of Differentiation-Related Gene-1 (DRG1) with Breast Cancer Survival and in Vitro Impact of DRG1 Suppression. Cancers 4 24213460
2024 Anti-metastatic Effects of Bee Venom and Melittin in Breast Cancer Cells by Upregulation of BRMS1 and DRG1 Genes. Chemical biology & drug design 3 39396919
2023 Inactivation of DRG1, encoding a translation factor GTPase, causes a recessive neurodevelopmental disorder. Genetics in medicine : official journal of the American College of Medical Genetics 2 37179472
2025 Ambient NO2 induces premature pulmonary senescence in rats: The role of the ROS-DRG1/CDK5 axis. Journal of environmental sciences (China) 0 42070819

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