Affinage

RPL27

Large ribosomal subunit protein eL27 · UniProt P61353

Round 2 corrected
Length
136 aa
Mass
15.8 kDa
Annotated
2026-04-28
86 papers in source corpus 17 papers cited in narrative 17 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RPL27 is a conserved structural component of the large ribosomal subunit whose flexible N-terminal tail extends into the peptidyl transferase center to stabilize tRNA substrates at the A and P sites—particularly through residue K4—and to facilitate 50S subunit assembly, although it is dispensable for the chemical step of peptide-bond formation itself (PMID:9677420, PMID:16285924, PMID:26475831). High-resolution cryo-EM structures place human RPL27 within the 60S subunit at the subunit interface, where it participates in metazoan-specific structural elaborations (PMID:23636399, PMID:25901680). In Firmicutes, the L27 precursor undergoes obligatory post-translational N-terminal cleavage by the cysteine protease Prp, a processing step essential for ribosome function in Staphylococcus aureus (PMID:25388641, PMID:40766419). Loss-of-function mutations in human RPL27 impair pre-rRNA processing and erythropoiesis, causing Diamond-Blackfan anemia (PMID:25424902).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1981 Medium

    The first question was where L27 sits on the ribosome: RNA–protein cross-linking placed L27 in direct contact with 23S rRNA near the peptidyl transferase center, establishing it as a PTC-proximal protein.

    Evidence Chemical cross-linking (2-iminothiolane + UV) with RNA fragment analysis in E. coli 50S subunits, replicated by a 1988 study with antibody-based purification

    PMID:3278299 PMID:6170935

    Open questions at the time
    • Cross-linking identifies proximity but not direct functional role
    • Resolution limited to ~5-nt RNA stretches
  2. 1987 Medium

    Immuno-EM provided three-dimensional confirmation that L27 localizes to the base of the central protuberance on the interface side of the 50S subunit, consistent with PTC proximity.

    Evidence Immuno-electron microscopy of 50S subunits using anti-L27 antibodies and reconstituted heterologous subunits

    PMID:3123891

    Open questions at the time
    • Low-resolution technique (~25 Å)
    • Does not reveal atomic contacts
  3. 1989 High

    Functional conservation of L27 across kingdoms was demonstrated when mouse RPL27 fully complemented a yeast L29-null strain, showing the ribosomal role is conserved from bacteria to mammals.

    Evidence Yeast genetic complementation with mouse L27' cDNA in an L29-null background

    PMID:2643099

    Open questions at the time
    • Does not address whether L27's PTC-proximal position is conserved in eukaryotes
    • Competitive incorporation when both L27 and L29 present was unexplained mechanistically
  4. 1998 High

    Deletion of rpmA established that L27 contributes to both 50S assembly and tRNA positioning: ΔrpmA E. coli accumulate a 40S assembly intermediate, show reduced peptidyl transferase activity, and impaired A-site tRNA binding.

    Evidence Chromosomal deletion with plasmid complementation, sucrose-gradient sedimentation, peptidyl transferase and tRNA binding assays in E. coli

    PMID:9677420

    Open questions at the time
    • Could not distinguish assembly defect from direct catalytic contribution
    • P-site binding appeared unaffected, creating an asymmetry not yet explained
  5. 2001 Medium

    Heterologous complementation with Aquifex aeolicus L27 showed that the assembly and translational functions of L27 are structurally separable: the heterologous protein rescued growth but not the 50S assembly defect.

    Evidence Heterologous expression in E. coli ΔrpmA, sucrose-gradient analysis, CD and NMR

    PMID:11673426

    Open questions at the time
    • Structural basis for the separation of assembly and catalytic roles not identified
    • Single heterologous pair tested
  6. 2004 High

    The crystal structure of L27 from T. thermophilus revealed an all-β-sheet fold with a highly positively charged surface, providing the first atomic framework for understanding its RNA interactions at the PTC.

    Evidence X-ray crystallography at 2.8 Å with MAD phasing

    PMID:15340170

    Open questions at the time
    • Structure determined in isolation, not in ribosomal context
    • Disordered N-terminal tail not resolved
  7. 2005 High

    Systematic N-terminal truncation showed that even removing three residues impairs growth, PTC activity, and P-site tRNA cross-linking, pinpointing the flexible N-terminal tail as the functionally critical element that reaches into the PTC.

    Evidence Graded N-terminal truncation mutagenesis in E. coli with peptidyl transferase assay and photoreactive tRNA cross-linking

    PMID:16285924

    Open questions at the time
    • Whether the tail contributes to catalysis or solely to substrate positioning was unresolved
  8. 2012 Medium

    Single-molecule FRET revealed that L27 N-terminal residues, especially K4, stabilize peptidyl-tRNA at the P site after translocation, directly measuring the dynamic consequence of tail loss.

    Evidence smFRET analysis of tRNA dynamics on ribosomes bearing L27 N-terminal mutants

    PMID:22930421

    Open questions at the time
    • Single-lab observation awaiting independent replication
    • Mechanism by which K4 contacts tRNA not structurally resolved
  9. 2013 High

    High-resolution cryo-EM of metazoan 80S ribosomes placed RPL27 in the eukaryotic 60S subunit for the first time and revealed metazoan-specific structural elaborations around it.

    Evidence Single-particle cryo-EM of human and Drosophila 80S ribosomes with atomic model building

    PMID:23636399

    Open questions at the time
    • Functional significance of metazoan-specific L27 extensions not tested
  10. 2014 High

    Two parallel advances linked L27 to disease and to post-translational processing: a de novo RPL27 splicing mutation was identified as causing Diamond-Blackfan anemia with impaired pre-rRNA processing and erythropoiesis, while in Firmicutes the cysteine protease Prp was shown to perform obligatory N-terminal cleavage of L27 required for viability in S. aureus.

    Evidence Whole-exome sequencing of DBA patient, siRNA knockdown with pre-rRNA assay, zebrafish morpholino model (DBA); mass spectrometry, genetic deletion/complementation (Prp/L27 processing in S. aureus)

    PMID:25388641 PMID:25424902

    Open questions at the time
    • Only a single DBA family reported for RPL27
    • Mechanism linking L27 haploinsufficiency specifically to erythroid lineage not resolved
    • Whether Prp processing is universal across all Firmicutes unclear
  11. 2015 High

    Rapid kinetics experiments definitively resolved the long-standing question of whether L27 is catalytically essential: peptide-bond formation rate at physiological pH is independent of L27, establishing the ribosome as the sole catalyst and relegating L27 to a substrate-positioning role.

    Evidence Stopped-flow fluorescence with aminoacyl-tRNA and puromycin on ΔL27 E. coli ribosomes, full pH titration

    PMID:26475831

    Open questions at the time
    • Whether L27 affects catalysis under specific stress conditions not tested
    • Contribution to accuracy/fidelity of translation not assessed
  12. 2023 Medium

    An extra-ribosomal function for RPL27 was uncovered in colorectal cancer, where RPL27 silencing suppressed proliferation, stemness, and xenograft growth through downregulation of PLK1 signaling.

    Evidence siRNA knockdown in HCT116/HT29 cells, RNA-seq, FACS, western blot, sphere-formation assay, xenograft model

    PMID:37387446

    Open questions at the time
    • Mechanism linking RPL27 to PLK1 expression or stability not identified
    • Cannot fully exclude indirect effects of global translation reduction upon ribosomal protein depletion
    • Single study, not yet independently replicated
  13. 2025 Medium

    Biochemical dissection of Prp-L27 processing in S. pneumoniae revealed that Prp acts as a dimer, that binding depends on the L27 cleavage-site residue F12, and that streptococcal L27 processing differs from staphylococcal processing in dispensability.

    Evidence In vitro protease assay, site-directed mutagenesis (PrpC34S, L27-F12A), overexpression toxicity and Δprp viability in S. pneumoniae

    PMID:40766419

    Open questions at the time
    • Species-specific differences in L27 processing requirements not fully rationalized
    • Structural basis for Prp dimerization and substrate recognition not resolved at atomic level

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key open questions include: the structural basis of the L27 N-terminal tail interaction with P-site tRNA in eukaryotes, the mechanism linking human RPL27 haploinsufficiency specifically to erythroid lineage failure in DBA, and whether the reported PLK1-dependent extra-ribosomal function reflects a direct RPL27 moonlighting activity or an indirect consequence of ribosome stress.
  • No eukaryotic structure with L27 N-terminus resolved at the PTC
  • Erythroid specificity mechanism unknown
  • Extra-ribosomal function not confirmed by independent labs

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 5 GO:0003723 RNA binding 4
Localization
GO:0005840 ribosome 7 GO:0005829 cytosol 2
Pathway
R-HSA-392499 Metabolism of proteins 6 R-HSA-1643685 Disease 2
Partners
PLK1PRPRPL16RPL20RPL21
Complex memberships
60S large ribosomal subunit80S ribosome

Evidence

Reading pass · 17 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 Deletion of rpmA (encoding ribosomal protein L27) in E. coli causes a 5–6-fold growth defect, accumulation of a 40S precursor to the 50S subunit deficient in proteins L16, L20, L21, and L27, 3–4-fold reduction in peptidyl transferase activity, and impaired enzymatic binding of Phe-tRNAPhe to the A site, while P-site binding of N-Ac-Phe-tRNAPhe is largely unperturbed. These results establish that L27 contributes to both 50S subunit assembly and tRNA positioning at the peptidyl transferase center. Chromosomal deletion, plasmid complementation, sucrose-gradient sedimentation, peptidyl transferase assay, A/P-site tRNA binding assays The Journal of biological chemistry High 9677420
1981 RNA–protein cross-linking in E. coli 50S subunits using 2-iminothiolane and UV irradiation placed protein L27 in contact with 23S rRNA positions 2332–2337, locating it near the peptidyl transferase center. Chemical cross-linking (2-iminothiolane) followed by UV irradiation, RNA fragment analysis Nucleic acids research Medium 6170935
1988 A second cross-linking study refined the L27 contact site on 23S rRNA to positions 2320–2323, confirming proximity of L27 to the peptidyl transferase center region. RNA–protein cross-linking (2-iminothiolane + UV), affinity chromatography with anti-L27 antibodies, RNA fragment sequencing Nucleic acids research Medium 3278299
1987 Immuno-electron microscopy using antibodies against E. coli L27 and reconstituted 50S subunits bearing B. subtilis L27 localized L27 to the base of the central protuberance on the interface side of the 50S particle, placing it in proximity to the peptidyl transferase center and revealing a significant depression in that region. Immuno-electron microscopy, 50S reconstitution with heterologous L27 Molecular & general genetics : MGG Medium 3123891
2004 Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8 determined at 2.8 Å resolution reveals an all-β-sheet fold consisting of two sets of four-stranded β-sheets around a hydrophobic core, with a highly positively charged surface consistent with RNA binding near the peptidyl transferase center. X-ray crystallography (multiwavelength anomalous dispersion) Protein science : a publication of the Protein Society High 15340170
2005 Systematic N-terminal truncation of L27 in E. coli shows that loss of as few as three N-terminal amino acids decreases growth rate, impairs peptidyl transferase activity, and reduces cross-linking of L27 to the 3′ end of a photoreactive P-site tRNA, establishing that the flexible N-terminal tail of L27 protrudes to the peptidyl transferase active site and contributes to tRNA substrate positioning. N-terminal truncation mutagenesis, peptidyl transferase assay, photoreactive tRNA cross-linking, growth rate measurement Molecular cell High 16285924
2008 Molecular dynamics simulations of the T. thermophilus ribosome predict that L27 N-terminus interacts with the A76 phosphate of A-site tRNA to assist substrate positioning, but deletion of L27 causes only a minor rate reduction for peptidyl transfer, supporting the ribozyme model while explaining the A-site binding defect. The N-terminal α-amino group of L27 is predicted to undergo a pKa downshift during the puromycin reaction but not with aminoacyl-tRNA, potentially explaining pH-dependence differences between these substrates. Molecular dynamics / QM/MM computer simulations of the peptidyl transfer reaction Biochemistry Low 18393533
2012 Single-molecule FRET analysis of tRNA dynamics in ribosomes bearing wild-type or N-terminal L27 mutants (ΔA2H3, ΔA2H3K4, or Δ9 residues) shows that removal of the first three N-terminal residues or mutation of residue K4 reduces formation of stable peptidyl-tRNA after translocation, establishing that L27 stabilizes the peptidyl-tRNA at the P site and that residue K4 is critical for this stabilization. Single-molecule fluorescence resonance energy transfer (smFRET), N-terminal truncation and point mutagenesis Protein science : a publication of the Protein Society Medium 22930421
2015 Fast-kinetics experiments (stopped-flow) show that the rate of peptide-bond formation at physiological pH—with either aminoacyl-tRNA or the substrate analog puromycin—is independent of the presence of L27 in E. coli ribosomes. Translation of natural mRNAs is only marginally affected by L27 absence. The pH dependence of the puromycin reaction is unaltered in the absence of L27, ruling out the N-terminal α-amine as the ionizing group in catalysis. L27 is also not required for peptidyl-tRNA hydrolysis during termination. These data establish that L27 is not a catalytic component of the peptidyl transferase center. Rapid kinetics (stopped-flow fluorescence), puromycin assay, natural mRNA translation assay, pH-dependence analysis, L27 deletion ribosomes RNA (New York, N.Y.) High 26475831
2001 Expression of Aquifex aeolicus L27 in an E. coli ΔrpmA strain partially rescues growth rate and is incorporated into E. coli ribosomes, but fails to rescue the 50S subunit assembly defect seen in ΔrpmA cells. This establishes that L27's role in ribosome assembly is structurally distinct from its role in completed ribosome function. Heterologous expression complementation, sucrose-gradient sedimentation, circular dichroism, NMR Journal of bacteriology Medium 11673426
2014 A cysteine protease (Prp) in Staphylococcus aureus and related Firmicutes performs post-translational N-terminal cleavage of ribosomal protein L27, removing an N-terminal extension (absent in most Gram-negative bacteria) that is present in the encoded form but absent from mature ribosomes. This processing is required for L27 function and viability in S. aureus. Mass spectrometry identification of cleavage, protease identification by bioinformatics and genetics, in vivo deletion/complementation Molecular microbiology High 25388641
2014 Whole-exome sequencing identified a de novo splicing mutation in RPL27 in a Diamond-Blackfan anemia (DBA) patient. In vitro siRNA-mediated knockdown of rpl27 disturbed pre-ribosomal RNA processing. Zebrafish rpl27 morphant models showed impaired erythrocyte production and developmental defects in tail and/or brain, establishing RPL27 as a DBA gene whose loss disrupts pre-rRNA processing and erythropoiesis. Whole-exome sequencing, in vitro siRNA knockdown with pre-rRNA processing assay, zebrafish morpholino knockdown with erythroid and developmental phenotyping British journal of haematology High 25424902
1989 Mouse ribosomal protein L27' (ortholog of human RPL27) can functionally substitute for yeast ribosomal protein L29 in vivo: when expressed in yeast cells devoid of L29, mouse L27' supports normal growth, demonstrating assembly into functional yeast ribosomes. However, when both proteins are present, yeast L29 is preferentially incorporated, indicating competitive assembly. Yeast genetic complementation (L29-null strain rescue), cycloheximide resistance assay, growth rate measurement Proceedings of the National Academy of Sciences of the United States of America High 2643099
2023 siRNA-mediated silencing of RPL27 in human colorectal cancer cell lines (HCT116, HT29) suppresses cell proliferation, induces G2/M arrest, and promotes apoptosis. RNA sequencing revealed that RPL27 knockdown downregulates PLK1 protein and G2/M regulators (phospho-CDC25C, CDK1, cyclin B1). RPL27 silencing also reduced migration, invasion, sphere-forming capacity of parental and CD133+ cancer stem cells, and CD133/PLK1 levels. In vivo xenograft growth was significantly inhibited, establishing an extra-ribosomal oncogenic function of RPL27 mediated through PLK1 signaling. siRNA knockdown, cell proliferation assay, FACS cell cycle analysis, RNA sequencing, western blotting, xenograft mouse model, sphere-formation assay International journal of oncology Medium 37387446
2013 High-resolution cryo-EM structures of human (Homo sapiens) and Drosophila melanogaster 80S ribosomes reveal the position of RPL27 within the large (60S) ribosomal subunit, showing co-evolution of metazoan-specific ribosomal RNA and proteins including RPL27 within two additional structural layers unique to metazoan ribosomes. Single-particle cryo-electron microscopy, atomic model building Nature High 23636399
2015 Near-atomic cryo-EM structure of the human 80S ribosome at 3.6 Å average resolution (2.9 Å in stable regions) provides detailed atomic context for human RPL27 within the large subunit, including interactions at the subunit interface that remodels during rotational movements. Single-particle cryo-electron microscopy, atomic model building Nature High 25901680
2025 In S. pneumoniae, the Prp cysteine protease forms dimers that efficiently cleave the N-terminal extension of L27 in vitro. An inactive Prp variant (PrpC34S) binds L27 without cleaving it, and an L27 mutation at the cleavage site (F12A) abolishes Prp binding. Overexpression of inactive PrpC34S is detrimental to S. pneumoniae growth. Unlike in S. aureus, the Δprp strain is viable (due to cleavage by another protease), and a strain lacking the L27 N-terminal extension shows impaired but viable growth, revealing important mechanistic differences between streptococcal and staphylococcal L27 processing. In vitro protease cleavage assay, site-directed mutagenesis (PrpC34S, L27-F12A), overexpression growth assay, genetic deletion (Δprp, ΔN-extension) bioRxiv / International journal for parasitology Medium 40766419

Source papers

Stage 0 corpus · 86 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1994 A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science (New York, N.Y.) 5070 7545954
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2012 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Molecular cell 973 22681889
2005 Nucleolar proteome dynamics. Nature 934 15635413
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2004 A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nature cell biology 841 14743216
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2002 Directed proteomic analysis of the human nucleolus. Current biology : CB 780 11790298
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2018 High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies. Molecular cell 580 29395067
2017 Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science (New York, N.Y.) 533 28302793
2011 Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation. Nature cell biology 490 21423176
2013 Structures of the human and Drosophila 80S ribosome. Nature 481 23636399
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2013 The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes. The Journal of biological chemistry 413 23463506
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2004 The molecular mechanics of eukaryotic translation. Annual review of biochemistry 396 15189156
2015 Structure of the human 80S ribosome. Nature 380 25901680
2015 Proteome-wide profiling of protein assemblies by cross-linking mass spectrometry. Nature methods 370 26414014
2010 Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics. Cell 318 21145461
2016 Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics. Cell reports 306 27342126
2009 Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. Cell 281 19167051
2004 Quaternary structure, protein dynamics, and synaptic function of SAP97 controlled by L27 domain interactions. Neuron 205 15504326
2014 Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia. British journal of haematology 83 25424902
2005 A protein component at the heart of an RNA machine: the importance of protein l27 for the function of the bacterial ribosome. Molecular cell 79 16285924
1981 The use of 2-iminothiolane as an RNA-protein cross-linking agent in Escherichia coli ribosomes, and the localisation on 23S RNA of sites cross-linked to proteins L4, L6, L21, L23, L27 and L29. Nucleic acids research 72 6170935
2004 The tetrameric L27 domain complex as an organization platform for supramolecular assemblies. Nature structural & molecular biology 70 15048107
1976 Isolation of eukaryotic ribosomal proteins. Purification and characterization of the 60 S ribosomal subunit proteins L4, L5, L7, L9, L11, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35', L37, and L39. The Journal of biological chemistry 70 1002715
2001 Enhanced expression of S8, L12, L23a, L27 and L30 ribosomal protein mRNAs in human hepatocellular carcinoma. Anticancer research 69 11724303
1977 Isolation of eukaryotic ribosomal proteins. Purification and characterization of 60 S ribosomal subunit proteins L3, L6, L7', L8, L10, L15, L17, L18, L19, L23', L25, L27', L28, L29, L31, L32, L34, L35, L36, L36', and L37'. The Journal of biological chemistry 68 863909
1988 RNA-protein cross-linking in Escherichia coli 50S ribosomal subunits; determination of sites on 23S RNA that are cross-linked to proteins L2, L4, L24 and L27 by treatment with 2-iminothiolane. Nucleic acids research 66 3278299
1998 Ribosomal protein L27 participates in both 50 S subunit assembly and the peptidyl transferase reaction. The Journal of biological chemistry 58 9677420
2008 Role of ribosomal protein L27 in peptidyl transfer. Biochemistry 37 18393533
2010 Central regulation of locomotor behavior of Drosophila melanogaster depends on a CASK isoform containing CaMK-like and L27 domains. Genetics 35 21059886
2004 Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes. The EMBO journal 34 15241471
2018 Purification and characterization of ribosomal proteins L27 and L30 having antimicrobial activity produced by the Lactobacillus salivarius SGL 03. Journal of applied microbiology 33 29159916
2005 A unified assembly mode revealed by the structures of tetrameric L27 domain complexes formed by mLin-2/mLin-7 and Patj/Pals1 scaffold proteins. Proceedings of the National Academy of Sciences of the United States of America 25 15863617
2002 Coordinated folding and association of the LIN-2, -7 (L27) domain. An obligate heterodimerization involved in assembly of signaling and cell polarity complexes. The Journal of biological chemistry 25 12110687
2007 Lin-7 targets the Kir 2.3 channel on the basolateral membrane via a L27 domain interaction with CASK. American journal of physiology. Cell physiology 24 17913842
1991 Ribosomal protein L27 is identical in chick and rat. Nucleic acids research 22 1709488
1979 Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli. Journal of bacteriology 22 378941
2014 Specific N-terminal cleavage of ribosomal protein L27 in Staphylococcus aureus and related bacteria. Molecular microbiology 21 25388641
1994 Phorbol ester induction of differentiation and apoptosis in the K562 cell line is accompanied by marked decreases in the stability of globin mRNAs and decreases in the steady state level of mRNAs encoding for ribosomal proteins L35, L31, L27, and L21. Cellular & molecular biology research 20 7804323
1989 Functional substitution of mouse ribosomal protein L27' for yeast ribosomal protein L29 in yeast ribosomes. Proceedings of the National Academy of Sciences of the United States of America 19 2643099
1988 Nucleotide sequence of cloned cDNA specific for rat ribosomal protein L27. European journal of biochemistry 17 2833393
1980 Purification of Drosophila ribosomal proteins. Isolation of proteins S8, S13, S14, S16, S19, S20/L24, S22/L26, S24, S25/S27, S26, S29, L4, L10/L11, L12, L13, L16, L18, L19, L27, 1, 7/8, 9, and 11. Biochemistry 16 6773542
2015 Activities of the peptidyl transferase center of ribosomes lacking protein L27. RNA (New York, N.Y.) 15 26475831
2012 Structure of an L27 domain heterotrimer from cell polarity complex Patj/Pals1/Mals2 reveals mutually independent L27 domain assembly mode. The Journal of biological chemistry 15 22337881
2002 Simultaneous horizontal gene transfer of a gene coding for ribosomal protein l27 and operational genes in Arthrobacter sp. Journal of molecular evolution 15 12486522
1994 Cloning and nucleotide sequence of a full length cDNA encoding ribosomal protein L27 from human fetal kidney. Biochimica et biophysica acta 14 8148381
2023 RPL27 contributes to colorectal cancer proliferation and stemness via PLK1 signaling. International journal of oncology 13 37387446
2006 Promoter shuffling at a nuclear gene for mitochondrial RPL27. Involvement of interchromosome and subsequent intrachromosome recombinations. Plant physiology 11 16603668
2010 Structural basis for tandem L27 domain-mediated polymerization. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 10 20702775
1993 Cloning and nucleotide sequencing of the genes, rpIU and rpmA, for ribosomal proteins L21 and L27 of Escherichia coli. DNA sequence : the journal of DNA sequencing and mapping 10 8312607
1992 Nuclear-encoded chloroplast ribosomal protein L27 of Nicotiana tabacum: cDNA sequence and analysis of mRNA and genes. Biochemistry 10 1339289
2012 Role of the ribosomal protein L27 revealed by single-molecule FRET study. Protein science : a publication of the Protein Society 9 22930421
2004 Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8. Protein science : a publication of the Protein Society 9 15340170
1987 Localization of ribosomal protein L27 at the peptidyl transferase centre of the 50 S subunit, as determined by immuno-electron microscopy. Molecular & general genetics : MGG 8 3123891
1988 Expression of naturally occurring RNA molecules complementary to the murine L27' ribosomal protein mRNA. Gene 7 2468568
2020 Identification and characterization of SaRpAMP, a 60S ribosomal protein L27-derived antimicrobial peptide from amur catfish, Silurus asotus. Fish & shellfish immunology 6 32711152
2018 Structures of the L27 Domain of Disc Large Homologue 1 Protein Illustrate a Self-Assembly Module. Biochemistry 6 29261291
2016 Coexpression of Escherichia coli obgE, Encoding the Evolutionarily Conserved Obg GTPase, with Ribosomal Proteins L21 and L27. Journal of bacteriology 6 27137500
2010 Independent evolution of a new allele of F1 pollen sterility gene S27 encoding mitochondrial ribosomal protein L27 in Oryza nivara. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 6 20878142
2014 Characterization and site-directed mutagenesis of a novel class II 5-enopyruvylshikimate-3-phosphate (EPSP) synthase from the deep-sea bacterium Alcanivorax sp. L27. Enzyme and microbial technology 5 25039062
2001 Differential effects of replacing Escherichia coli ribosomal protein L27 with its homologue from Aquifex aeolicus. Journal of bacteriology 5 11673426
2024 Identification of a novel antimicrobial peptide from amphioxus ribosomal protein L27. Fish & shellfish immunology 4 39622458
2003 Trypanosoma cruzi: identification and characterization of a novel ribosomal protein L27 (TcrL27) that cross-reacts with an affinity-purified anti-Sm antibody. Parasitology 4 12866796
2024 The L27 domain of MPP7 enhances TAZ-YY1 cooperation to renew muscle stem cells. EMBO reports 3 39496834
2023 Effect of RPL27 knockdown on the proliferation and apoptosis of human liver cancer cells. Biochemical and biophysical research communications 3 37812860
2004 Ac His1 [D-Phe2, K15, R16, L27] VIP (3-7)/GRF (8-27)--a VPAC1 receptor antagonist--is an inverse agonist on two constitutively active truncated VPAC1 receptors. Peptides 3 15501526
2011 Crystallization and preliminary X-ray data collection of the L27(PATJ)-(L27N,L27C)(Pals1)-L27(MALS) tripartite complex. Acta crystallographica. Section F, Structural biology and crystallization communications 1 22102253
1994 The gene for ribosomal protein L27 is located on the plastid rather than the nuclear genome of the chlorophyll c-containing alga Pleurochrysis carterae. Plant molecular biology 1 8111025
2025 Cleavage of Streptococcus pneumoniae ribosomal protein L27 by the Prp protease. bioRxiv : the preprint server for biology 0 40766419
2025 Ribosomal protein L27 contributes to virulence and maduramicin resistance in Eimeria tenella. International journal for parasitology. Drugs and drug resistance 0 41004933
2024 De Novo Variant in the RPL27 Gene in a Second Infant with Diamond-Blackfan Anemia. Case reports in hematology 0 38988374
2024 Identification of Genes Associated with Familial Focal Segmental Glomerulosclerosis Through Transcriptomics and In Silico Analysis, Including RPL27, TUBB6, and PFDN5. International journal of molecular sciences 0 39519211
2023 The L27 Domain of MPP7 enhances TAZ-YY1 Cooperation to Renew Muscle Stem Cells. bioRxiv : the preprint server for biology 0 37961392
2023 The L27 Domain of MPP7 enhances TAZ-YY1 Cooperation to Renew Muscle Stem Cells. Research square 0 38077061