Affinage

RAB35

Ras-related protein Rab-35 · UniProt Q15286

Length
201 aa
Mass
23.0 kDa
Annotated
2026-04-28
94 papers in source corpus 52 papers cited in narrative 52 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAB35 is a plasma membrane– and endosome-associated small GTPase that functions as a master coordinator of endocytic recycling, membrane identity, and actin remodeling across multiple cellular contexts including cytokinesis, cell polarity, ciliogenesis, phagocytosis, neurite outgrowth, and myelination. In its GTP-bound state, RAB35 recruits a context-dependent set of effectors—OCRL for PtdIns(4,5)P₂ hydrolysis and F-actin clearance at the intercellular bridge and newborn endosomes, ACAP2 for Arf6 inactivation during phagocytosis, neurite outgrowth, and Weibel-Palade body exocytosis, fascin for actin bundling, Hrs/NDP52 for ESCRT-dependent degradation of synaptic vesicle proteins and selective autophagy, MICAL-L1 for downstream Rab cascade scaffolding, and myotubularin phosphatases (MTMR13/MTMR2) for PI 3-phosphate turnover and mTORC1 suppression during myelin growth (PMID:16950109, PMID:21706022, PMID:26725203, PMID:22045739, PMID:19729655, PMID:27535913, PMID:28848034, PMID:25086062, PMID:32503983). RAB35 is activated by DENN-domain GEFs (DENND1A/B/C, DENND2B, FLCN) and inactivated by TBC-domain GAPs (TBC1D10A/B/C, TBC1D13), forming an ARF6-antagonistic GTPase cascade that controls surface receptor sorting, cadherin stabilization, clathrin-independent cargo fate, and epithelial polarity establishment (PMID:20159556, PMID:22226746, PMID:25988331, PMID:27040773, PMID:31992598, PMID:37454296). A homozygous R27H missense variant in the RAB35 GTPase domain causes a neurodevelopmental disorder characterized by enhanced GEF binding, reduced effector engagement, Arf6 hyperactivation, and defective cytokinesis and ciliogenesis (PMID:38432637).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2006 High

    Establishing RAB35 as a plasma membrane/endosomal GTPase required for fast endocytic recycling and cytokinesis resolved the question of where this poorly characterized Rab functions and provided the first cellular phenotype (failed abscission, vacuolar marker accumulation) linking it to membrane traffic.

    Evidence Dominant-negative/GTP-locked mutant overexpression with fluorescence microscopy and endocytic marker trafficking in human cells

    PMID:16950109

    Open questions at the time
    • Identity of Rab35 activators and inactivators unknown
    • Direct effectors mediating cytokinesis and recycling not identified
    • Mechanism of PIP2/septin bridge localization unclear
  2. 2008 High

    Identification of the DENN-domain protein RME-4 as a RAB-35 activator at clathrin-coated pits in C. elegans, and EPI64C/TBC1D10C as a mammalian Rab35 GAP, established the first GEF and GAP for RAB35 and placed it in the clathrin-mediated endocytic pathway upstream of RAB-7 and parallel to RAB-11.

    Evidence C. elegans genetic epistasis, yeast two-hybrid, and in vitro GAP assay with siRNA knockdown in Jurkat T cells

    PMID:18354496 PMID:18450757

    Open questions at the time
    • Mammalian DENN-domain GEFs not yet tested
    • Whether GAP identity varies by cell type unknown
    • Structural basis for GEF/GAP recognition undefined
  3. 2009 High

    Discovery that fascin is a direct Rab35 effector linking active Rab35 to actin bundling—demonstrated by ectopic mitochondrial targeting—answered how Rab35 remodels the actin cytoskeleton, while parallel work showed Rab35 activates Cdc42 for neurite outgrowth.

    Evidence Direct pulldown, ectopic mitochondrial recruitment in cultured cells, Drosophila bristle genetics; Cdc42 activation assays and siRNA in PC12/N1E-115 cells

    PMID:19289122 PMID:19729655

    Open questions at the time
    • Whether fascin and Cdc42 pathways are independent or connected unknown
    • Structural basis of Rab35-fascin recognition unresolved
  4. 2010 High

    Biochemical demonstration that the DENN domains of DENND1A, DENND1B, and DENND1C all catalyze GDP-to-GTP exchange on Rab35 defined the mammalian GEF family, while revealing their differential binding properties and clathrin/AP-2 association linked Rab35 activation to clathrin-coated pit dynamics.

    Evidence In vitro GEF assays, yeast two-hybrid, siRNA knockdown with endosome morphology analysis

    PMID:20154091 PMID:20159556

    Open questions at the time
    • Physiological contexts requiring each GEF isoform not determined
    • Regulation of GEF recruitment itself unresolved
  5. 2011 High

    Identification of OCRL as a direct Rab35 effector at the intercellular bridge explained how Rab35 controls PtdIns(4,5)P₂ hydrolysis and F-actin clearance for cytokinetic abscission, while parallel studies identified ACAP2 as an effector coupling Rab35 to Arf6 inactivation during phagocytosis and MICAL-L1 as a recycling endosome partner.

    Evidence GST pulldown (direct binding), siRNA depletion, PtdIns(4,5)P₂ and F-actin imaging in human cells and Lowe patient lines; phagocytic cup live imaging in macrophages; co-IP for MICAL-L1

    PMID:21706022 PMID:21951725 PMID:22045739

    Open questions at the time
    • How Rab35 selects among multiple effectors at different locations unclear
    • Whether OCRL disease mutations specifically disrupt Rab35 binding not tested
  6. 2012 High

    Dissection of the ARF6→EPI64B⊣RAB35 GTPase cascade revealed that ARF6 negatively regulates Rab35 loading at clathrin-coated pits, while Rab35 reciprocally suppresses Arf6 via ACAP2, establishing a mutual antagonism that governs integrin/cadherin surface balance, cell migration, and GLUT4 trafficking.

    Evidence Constitutive active/inactive mutant epistasis, GAP activity assays, cadherin/integrin trafficking, GLUT4 translocation in adipocytes, TBC1D13 in vitro GAP screen

    PMID:22226746 PMID:22762500 PMID:23197472 PMID:23264734

    Open questions at the time
    • Whether Rab35-Arf6 antagonism operates identically in all cell types unclear
    • Structural basis for EPI64B selectivity for Rab35 versus other Rabs unresolved
  7. 2014 High

    MICAL-L1 was established as a Rab35 effector scaffold that recruits Rab8/13/36 to Arf6-positive endosomes for non-redundant neurite outgrowth regulation, while in oligodendrocytes the Rab35→ACAP2⊣Arf6 cascade was shown to suppress differentiation, revealing cell-type-specific functional switching.

    Evidence siRNA knockdown/rescue epistasis in PC12 cells; GTPase activity measurements and myelination co-culture in oligodendrocytes

    PMID:24600047 PMID:25086062

    Open questions at the time
    • Which Rab35 effectors operate in oligodendrocyte differentiation besides ACAP2 unknown
    • How developmental signals modulate Rab35 activity level unresolved
  8. 2015 High

    TIRF imaging resolved the precise temporal sequence—DENND1A arrives, EPI64B departs, Rab35 activates, OCRL is recruited—on newborn endosomes within seconds of clathrin-coated vesicle scission, establishing the earliest post-endocytic event controlled by Rab35 and explaining CI-MPR sorting.

    Evidence Live TIRF microscopy, siRNA depletion, direct binding, cargo trafficking assays

    PMID:26725203

    Open questions at the time
    • Whether the same temporal sequence applies at all CCV populations unclear
    • How Rab35 is removed from maturing endosomes not defined
  9. 2016 High

    RAB35 was shown to directly bind the cytoplasmic tail of Podocalyxin and tether apical-determinant vesicles at the cleavage site, coupling cytokinesis to apico-basal polarity initiation in 3D epithelial cysts; separately, Hrs was identified as a neuronal Rab35 effector linking activity-dependent synaptic vesicle protein degradation to the ESCRT pathway.

    Evidence 3D MDCK cyst cultures with direct pulldown and polarity marker imaging; Co-IP and neuronal activity stimulation in hippocampal neurons

    PMID:27040773 PMID:27535913

    Open questions at the time
    • Whether Podocalyxin binding competes with other effectors at the bridge unresolved
    • Upstream signals activating Rab35 during neuronal stimulation not identified
  10. 2017 High

    NDP52 was identified as a direct Rab35 effector that promotes selective autophagy (xenophagy, mitophagy) and autophagosome maturation, with TBC1D10A opposing and TBK1 stimulating this process, broadening Rab35 function beyond recycling into degradative pathways.

    Evidence Direct binding assay, Co-IP, siRNA knockdown, bacterial infection and mitophagy models

    PMID:28848034

    Open questions at the time
    • Whether Rab35-NDP52 interaction is constitutive or stimulus-regulated unclear
    • Quantitative contribution of Rab35 versus other Rabs to NDP52 recruitment not determined
  11. 2018 High

    LRRK2 was shown to phosphorylate RAB35, and the PD-linked LRRK2 G2019S mutation enhances α-synuclein propagation through this phosphorylation, placing RAB35 as a key downstream mediator in Parkinson's disease pathobiology.

    Evidence Phosphorylation assays, LRRK2 kinase inhibitor treatment, epistasis in C. elegans and rodent models

    PMID:30150626

    Open questions at the time
    • Phosphorylation site(s) on Rab35 and their effect on effector selectivity not fully mapped
    • Whether Rab35 phosphorylation affects all effector interactions or is selective unclear
  12. 2019 High

    RAB35 was localized to primary cilia along with its GEF DENND1B and GAP TBC1D10A, and shown to regulate cilium length, Smoothened targeting, and Arl13b/INPP5E composition, with zebrafish morphants displaying left-right asymmetry defects—establishing Rab35 in ciliogenesis.

    Evidence siRNA knockdown, zebrafish morpholinos, GFP localization, ciliary membrane composition analysis

    PMID:31432619

    Open questions at the time
    • Whether Rab35 acts inside the cilium or at the ciliary base unresolved
    • Effector mediating Smoothened ciliary targeting not identified
  13. 2020 High

    A Rab35–MTMR13–MTMR2 complex was defined as the mechanism controlling PI 3-phosphate turnover and mTORC1 suppression in Schwann cells; Rab35 knockout in vivo caused focal hypermyelination rescued by mTORC1 inhibition, revealing a lipid-signaling axis for myelin growth control.

    Evidence Co-IP, in vivo Rab35 KO, PI phospholipid measurement, mTORC1 activity assay, pharmacological rescue

    PMID:32503983

    Open questions at the time
    • Whether Rab35-myotubularin axis operates in other myelinating glia unclear
    • Direct structural basis for Rab35-MTMR13 interaction unresolved
  14. 2023 High

    DENND2B was identified as a dual GEF and effector for Rab35 at the intercellular bridge, controlling ESCRT-III recruitment and the Aurora B abscission checkpoint; separately, context-specific GEF usage was defined (DENND1A for 3D polarity via ACAP2; FLCN for 2D OCRL-dependent sorting), revealing modular GEF-effector wiring.

    Evidence GEF assay, Co-IP, siRNA knockdown with cytokinesis and ESCRT-III imaging; CRISPR KO in 2D/3D MDCK cultures

    PMID:31992598 PMID:37454296

    Open questions at the time
    • How cells select among GEFs at the intercellular bridge not determined
    • Whether DENND2B dual function is unique or shared with other DENN proteins unclear
  15. 2024 High

    A human homozygous R27H variant in RAB35 was shown to cause neurodevelopmental disease by trapping RAB35 in an inactive conformation (enhanced GEF binding, reduced effector engagement), with consequent Arf6 hyperactivation, cytokinesis delay, and ciliary defects—providing the first Mendelian disease link and unifying multiple RAB35 functions in a single pathogenic mechanism.

    Evidence Exome sequencing, in vitro GEF and effector binding assays, Arf6 activation measurement, cytokinesis and cilia analysis

    PMID:38432637

    Open questions at the time
    • Number and spectrum of RAB35-associated disease families remain limited
    • Whether other hypomorphic variants produce milder phenotypes unknown
  16. 2024 High

    Congenital Rab35 KO is embryonic lethal (E7.5) in mice, and brain-specific KO causes hippocampal lamination defects and behavioral abnormalities, confirming essential in vivo roles; the Shigella effector IcsB was shown to non-canonically acylate Rab35 in its polybasic region to entrap it at bacterial vacuoles.

    Evidence Rab35 KO mice (congenital and brain-specific), behavioral testing, quantitative proteomics; STED microscopy and PTM analysis during Shigella infection

    PMID:37085665 PMID:38568808

    Open questions at the time
    • Which Rab35 effectors are critical for embryonic survival unknown
    • Whether acylation-based hijacking occurs with other pathogens not explored

Open questions

Synthesis pass · forward-looking unresolved questions
  • A comprehensive structural model of how Rab35 discriminates among its >10 effectors in a spatiotemporal manner is lacking; the phosphorylation code imposed by LRRK2 and potentially other kinases, and how it reshapes effector selectivity, remains undefined.
  • No co-crystal structure of Rab35 with any effector exists
  • Complete phosphorylation site mapping and functional consequences not determined
  • How Rab35 is partitioned between plasma membrane, endosomes, cilia, and intercellular bridge simultaneously is mechanistically unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003924 GTPase activity 7 GO:0098772 molecular function regulator activity 7
Localization
GO:0005768 endosome 4 GO:0005856 cytoskeleton 3 GO:0005886 plasma membrane 3 GO:0005929 cilium 2
Pathway
R-HSA-5653656 Vesicle-mediated transport 6 R-HSA-1640170 Cell Cycle 4 R-HSA-1266738 Developmental Biology 3 R-HSA-162582 Signal Transduction 3 R-HSA-168256 Immune System 3 R-HSA-1852241 Organelle biogenesis and maintenance 2 R-HSA-9612973 Autophagy 1
Partners
ACAP2DENND1ADENND1BDENND2BFSCN1MICAL1OCRLTBC1D10A

Evidence

Reading pass · 52 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2006 Rab35 localizes to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway; inhibition of Rab35 leads to failed cytokinesis with accumulation of endocytic markers on cytoplasmic vacuoles, and Rab35 is required for intercellular bridge localization of PIP2 and septin SEPT2 during cytokinesis. Dominant-negative/GTP-locked mutant overexpression, fluorescence microscopy, endocytic marker trafficking assays in human cells Current biology : CB High 16950109
2008 In C. elegans, RME-4 (a DENN domain protein) binds RAB-35 in its GDP-loaded conformation and is enriched on clathrin-coated pits; RME-4 recruits RAB-35 to coated pits/vesicles, and RAB-35 in turn promotes receptor recycling from early endosomes. Genetic epistasis shows RME-4 and RAB-35 function downstream of clathrin and upstream of RAB-7, acting synergistically with RAB-11 and RME-1. C. elegans genetic screens, yeast two-hybrid, GFP localization, epistasis analysis The EMBO journal High 18354496
2008 Rab35 is recruited to the immunological synapse (IS) in T cells; EPI64C (TBC1D10C) functions as a Rab35-GAP both in vitro and in transfection assays. Dominant-negative Rab35 or EPI64C impair transferrin recycling in T cells and TCR enrichment at the IS, and conjugate formation is impaired by both Rab35-DN and EPI64C. In vitro GAP assay, transfection of DN constructs, co-localization microscopy, siRNA knockdown in Jurkat T cells The Journal of biological chemistry High 18450757
2009 Rab35 directly associates with the actin-bundling protein fascin as an effector; active Rab35 recruits fascin to actin filaments and targeting Rab35 to the outer mitochondrial membrane triggers ectopic actin recruitment. Rab35 regulates actin filament assembly during Drosophila bristle development and filopodia formation in cultured cells. Direct binding assay (pulldown), ectopic mitochondrial targeting, Drosophila genetics, cell culture filopodia assay Science (New York, N.Y.) High 19729655
2009 Rab35 colocalizes with Cdc42 and can activate Cdc42 both in vivo and in vitro; activated Rab35 stimulates neurite outgrowth in PC12 and N1E-115 cells via a Cdc42-dependent pathway, and siRNA knockdown of Rab35 abolishes neurite outgrowth. In vitro Cdc42 activation assay, siRNA knockdown, neurite outgrowth quantification, co-localization microscopy FEBS letters Medium 19289122
2010 The DENN domain of connecdenn/DENND1A functions as a guanine nucleotide exchange factor (GEF) for Rab35; loss of Rab35 activity causes enlargement of early endosomes, inhibits MHC class I recycling, and prevents early endosomal recruitment of EHD1. In vitro GEF assay, siRNA knockdown, endosome morphology analysis, cargo recycling assay Molecular cell High 20159556
2010 Connecdenn 2 (DENND1B) also functions as a Rab35 GEF through its DENN domain; all three connecdenns (DENND1A-C) function as Rab35 GEFs with different activity levels. The DENN domains of connecdenn 1 and 2 bind Rab35, whereas connecdenn 3 does not, showing that Rab35 binding and activation are separable functions. All three connecdenns bind clathrin and AP-2 through their C-termini. In vitro GEF assay, yeast two-hybrid, siRNA knockdown, endosome morphology The Journal of biological chemistry High 20154091
2010 Rab35 mediates vesicle transport required for phagocytosis in Drosophila; Rab35-dependent recruitment of Cdc42 and Rac1 to sites of filopodium and lamellipodium formation occurs via microtubule tracks. Drosophila genetics, live-cell imaging, immunofluorescence in Drosophila S2 cells Molecular and cellular biology Medium 20065041
2011 GTP-bound (active) Rab35 directly interacts with OCRL (a PtdIns(4,5)P2 5-phosphatase mutated in Lowe syndrome) and controls its localization at the intercellular bridge during cytokinesis; Rab35 or OCRL depletion inhibits cytokinesis abscission with local PtdIns(4,5)P2 and F-actin accumulation, and OCRL acts as an effector of Rab35 to remodel F-actin for abscission. Direct binding assay (GST pulldown), siRNA depletion, PtdIns(4,5)P2 imaging, F-actin quantification, cytokinesis assays in human cells and Lowe patient cell lines Nature cell biology High 21706022
2011 MICAL-L1 is a Rab35-binding partner on tubular recycling endosomes; active Rab35 impairs MICAL-L1 recruitment to tubular recycling endosomes while Rab35 depletion promotes enhanced MICAL-L1 localization, indicating Rab35 is an upstream regulator of MICAL-L1. Arf6 also forms a complex with MICAL-L1 for recruitment to tubular endosomes. Co-immunoprecipitation, overexpression and depletion studies, fluorescence microscopy Traffic (Copenhagen, Denmark) Medium 21951725
2011 Rab35 is required for the early stage of FcγR-mediated phagocytosis in macrophages; active Rab35 recruits ACAP2 (an ARF6 GAP) to the phagocytic cup, and this GTP-Rab35/ACAP2 interaction is required for actin-dependent pseudopod extension and phagosome formation. siRNA knockdown, live-cell imaging, expression of GTP/GDP-locked mutants, pulldown assay, co-localization microscopy in macrophages Journal of cell science High 22045739
2011 Connecdenn 3 (DENND1C) directly binds actin via a unique C-terminal motif not found in connecdenn 1 or 2, coupling Rab35 activation to the actin cytoskeleton; connecdenn 3 co-localizes with fascin and actin filaments, identifying it as the relevant GEF for Rab35-mediated actin regulation and membrane protrusion formation. Direct actin-binding assay, GEF assay, co-localization microscopy, cell morphology analysis Molecular biology of the cell High 22072793
2011 A genome-wide RUN domain screen identified RUSC2 as a GTP-Rab35-specific binding protein; the minimal Rab35-binding site of RUSC2 (residues 982-1199) was defined and used to develop RBD35, a GTP-Rab35 trapper that specifically pulls down active Rab35 and inhibits neurite outgrowth when overexpressed. Yeast two-hybrid genome-wide screen, in vitro pull-down, PC12 cell overexpression assay Cell structure and function Medium 21737958
2012 ARF6 negatively regulates Rab35 activation via the Rab35 GAP EPI64B, which acts as an ARF6 effector; constitutively active ARF6 causes identical endocytic recycling and cytokinesis defects as GDP-Rab35. This ARF6/Rab35 GTPase cascade operates at clathrin-coated pits where activated ARF6 reduces Rab35 loading into the endocytic pathway. Constitutively active/inactive mutant expression, GAP activity assays, cytokinesis assays, endocytic recycling assays in human cells Current biology : CB High 22226746
2012 Rab35 functions as a Rab35 effector and Arf6-GAP coupling mechanism through centaurin-β2 (ACAP2): Rab35 accumulates at Arf6-positive endosomes upon NGF stimulation and recruits centaurin-β2 in a Rab35-dependent manner; the Arf6-GAP activity of centaurin-β2 at these endosomes is indispensable for NGF-induced neurite outgrowth in PC12 cells. siRNA knockdown, rescue experiments, live-cell imaging, co-localization assays in PC12 cells Journal of cell science High 22344257
2012 Rab35 suppresses Arf6 activity to downregulate Arf6-dependent recycling of β1-integrin and EGF receptors, while simultaneously maintaining cadherins at the cell surface; Rab35 knockdown promotes cell migration consistent with epithelial-mesenchymal transition by inverting this balance. siRNA knockdown, cell adhesion and migration assays, cargo recycling assays, co-immunoprecipitation Journal of cell science High 23264734
2012 Rab35 regulates cadherin trafficking and stabilization at cell-cell contacts in myoblasts and HeLa cells; Rab35 is required for N- and M-cadherin recruitment to cell contacts, their association with p120 catenin, and for PIP5KIγ accumulation at contacts for PIP2 production. Rab35 knockdown impairs myoblast fusion. siRNA knockdown, dominant-negative expression, fluorescence microscopy, co-immunoprecipitation, myoblast fusion assay Molecular biology of the cell High 23197472
2012 In Drosophila tracheal terminal cells, Rab35 and its apical membrane-localized GAP Whacked polarize seamless tube growth along the proximodistal axis; loss of Whacked (or constitutive Rab35 activation) leads to tube overgrowth at branch tips, while overexpression of Whacked or dominant-negative Rab35 causes ectopic tubes near the nucleus. Drosophila genetics (loss-of-function mutants), live imaging, epistasis analysis Nature cell biology High 22407366
2012 TBC1D13 functions as a Rab35-specific GAP in vitro; overexpression of constitutively active Rab35 (but not Rab10) reverses the TBC1D13-dependent block in insulin-stimulated GLUT4 translocation in adipocytes, implicating Rab35 in the insulin-stimulated GLUT4 trafficking pathway. In vitro GAP activity screen, yeast two-hybrid, constitutively active mutant rescue, GLUT4 translocation assay in adipocytes Traffic (Copenhagen, Denmark) High 22762500
2014 Rab35 promotes neurite outgrowth via MICAL-L1 as a scaffold: Rab35 determines the intracellular localization of MICAL-L1, which in turn recruits Rab8, Rab13, and Rab36 to Arf6-positive recycling endosomes. Each downstream Rab regulates neurite outgrowth non-redundantly downstream of Rab35 and MICAL-L1. Functional ablation (siRNA), rescue experiments, co-localization microscopy, neurite outgrowth quantification in PC12 cells Biology open High 25086062
2014 Rab35 and its effector ACAP2 negatively regulate oligodendrocyte differentiation and myelination by inactivating Arf6; as differentiation initiates, Rab35 and ACAP2 activities decrease while Arf6 activity increases. Arf6 knockdown inhibits differentiation, placing Rab35/ACAP2 upstream of Arf6 in a GTPase switching mechanism. siRNA knockdown, GTPase activity assays, morphological differentiation scoring, myelination co-culture Molecular biology of the cell High 24600047
2015 Rab35 is activated on newborn endosomes immediately after CCV scission via precise spatial-temporal loading: DENND1A is recruited first, then EPI64B disappears, allowing Rab35 activation; active Rab35 then directly recruits the OCRL phosphatase for post-scission PtdIns(4,5)P2 hydrolysis. Depletion of Rab35 or OCRL leads to retention of CI-MPR in peripheral clathrin-positive PtdIns(4,5)P2-rich endosomes. Live TIRF microscopy, siRNA depletion, cargo trafficking assays, direct binding assay, PtdIns(4,5)P2 imaging Current biology : CB High 26725203
2015 Rab35 controls endosomal sorting of clathrin-independent endocytosis (CIE) cargo; Rab35 delivered via clathrin-mediated endocytosis inactivates Arf6 through ACAPs (Arf6 GAPs), maintaining tubular endosomal networks and preventing lysosomal degradation of CIE cargos (CD44, CD98, CD147). siRNA knockdown of Rab35 recreates the altered trafficking phenotype of CME inhibition. siRNA knockdown, CME inhibition, cargo trafficking assays, Arf6-GTP measurement, rescue by Rab35 or ACAPs expression Traffic (Copenhagen, Denmark) High 25988331
2015 Two threonine residues (Thr-76 and Thr-81) in the switch II region of Rab35 are specifically required for binding ACAP2 (centaurin-β2) but dispensable for other Rab35 interactions; two asparagine residues (Asn-610 and Asn-691) in ACAP2 are key for Rab35 recognition. Binding-deficient mutants of either protein fail to support neurite outgrowth in PC12 cells. Deletion/point mutagenesis, pulldown assays, knockdown-rescue in PC12 cells The Journal of biological chemistry High 25694427
2016 Rab35 directly couples cytokinesis with initiation of apico-basal polarity in 3D MDCK cyst cultures by tethering intracellular vesicles containing apical determinants (aPKC, Cdc42, Crumbs3, Podocalyxin) at the cleavage site through a direct interaction between Rab35 and the cytoplasmic tail of Podocalyxin. Rab35 inactivation leads to complete polarity inversion in 3D cysts. 3D MDCK cyst cultures, dominant-negative expression, direct binding (pulldown), co-localization microscopy, polarity marker imaging Nature communications High 27040773
2016 Neuronal activity drives Rab35 activation and binding to the ESCRT-0 protein Hrs, identified as a novel Rab35 effector; Rab35 activation and Hrs binding recruit downstream ESCRT machinery to synaptic vesicle pools to initiate activity-dependent SV protein degradation. Co-immunoprecipitation, siRNA knockdown, live-cell imaging, neuronal activity stimulation in rat hippocampal neurons The Journal of neuroscience : the official journal of the Society for Neuroscience High 27535913
2016 Rab35 promotes axon elongation in primary neurons; PRPK (p53-related protein kinase) negatively regulates axonal elongation by targeting Rab35 for ubiquitin-proteasome degradation, and MAP1B interacts with PRPK to prevent this degradation. Rab35 also regulates Cdc42 activity in neurons. siRNA knockdown, ubiquitin-proteasome pathway assays, MAP1B KO neurons, rescue by Rab35 overexpression or PRPK inactivation, epistasis analysis The Journal of neuroscience : the official journal of the Society for Neuroscience High 27383602
2016 Rab35 in Drosophila epithelium is dynamically planar-polarized, enriched at contractile interfaces of intercalating cells; disruption of Rab35 function allows oscillations but prevents ratchet-like stabilization of contractions, so interfaces fail to shorten. Rab35 compartment initiation does not require Myosin II but compartments fail to terminate without actomyosin. Drosophila genetics, live imaging, dominant-negative expression, Myosin II perturbation Nature communications High 28883443
2017 GTP-bound Rab35 directly binds and recruits the autophagy receptor NDP52 to bacteria-containing endosomes and promotes NDP52 interaction with ubiquitin; TBC1D10A (GAP) inhibits this process while TBK1 (associated with NDP52) stimulates it. Rab35 also promotes NDP52 recruitment to damaged mitochondria and autophagosomes for mitophagy and autophagosome maturation. Direct binding assay, Co-IP, siRNA knockdown, bacterial infection model, mitophagy assay The EMBO journal High 28848034
2017 Rab35 is required for evoked Weibel-Palade body (WPB) exocytosis in endothelial cells; TBC1D10A is its GAP in this context. Rab35 acts through ACAP2 as an effector, and ACAP2 negatively regulates WPB exocytosis, while Arf6 (downstream of ACAP2) supports exocytosis. Genome-wide RabGAP screen, RabGAP overexpression, pulldown, co-IP, siRNA knockdown, WPB exocytosis assay The Journal of biological chemistry High 28566286
2018 LRRK2 kinase phosphorylates RAB35 to modulate α-synuclein propagation; the PD-linked G2019S mutation in LRRK2, which increases kinase activity, enhances α-synuclein propagation efficiency. Constitutive activation of RAB35 overrides reduced α-synuclein propagation in lrk-1 mutant C. elegans, placing RAB35 downstream of LRRK2 in this pathway. Cell culture, C. elegans genetics, rodent model, LRRK2 kinase inhibitor treatment, phosphorylation assays, epistasis Nature communications High 30150626
2018 RAB35 is required for apoptotic cell recognition and phagosome maturation in C. elegans as a third, parallel pathway to CED-1 and CED-5; RAB-35 promotes the phagosomal PI(4,5)P2-to-PI(3)P shift and recruits RAB-5 to phagosomal surfaces. GEFs FLCN-1 and RME-4, and GAP TBC-10, regulate RAB-35 in this context. C. elegans genetic screen, epistasis analysis, phosphoinositide imaging, phagosome maturation assays PLoS genetics High 30138370
2018 In C. elegans linker cell-type death (LCD), RAB-35 GTPase coordinates competitive phagocytosis onset and cell degradation; RAB-35 binds CNT-1, an ARF-6 GAP, and removes ARF-6 from phagosome membranes, facilitating PI(4,5)P2 removal and promoting phagolysosome maturation. Microfluidics-based long-term in vivo imaging, C. elegans genetics, phosphoinositide imaging, epistasis analysis Developmental cell High 30220571
2018 RAB35 directly regulates p85/PI3K activity to control oscillatory circular dorsal ruffles (CDRs) required for chemotactic migration; RAB35 is necessary and sufficient for growth factor-induced CDR waves and essential for chemotactic migration and chemoinvasion. RNAi screen, live-cell imaging, PI3K activity assay, chemotaxis assay, CDR quantification Nature communications High 29662076
2019 Rab35 controls cilium length, function, and membrane composition; Rab35 localizes to cilia along with its GEF (DENND1B) and GAP (TBC1D10A) regulators. Rab35 promotes ciliary targeting of Smoothened, limits ciliary accumulation of Arl13b and INPP5E, regulates ciliary PI(4,5)P2 levels, and interacts with Arl13b. Loss of Rab35 causes left-right asymmetry defects in zebrafish. siRNA knockdown, morpholinos (zebrafish), knockout, GFP localization, ciliary membrane composition analysis EMBO reports High 31432619
2019 IL-17A induces recruitment of Rab35 and DENND1C (GEF) to the IL-17R/Act1 complex in airway smooth muscle cells, activating Rab35; Rab35 activation is required for PKCα activation and fascin phosphorylation at Ser39, allowing F-actin/myosin stress fiber formation and enhanced smooth muscle contraction. Co-IP, siRNA knockdown, PKCα inhibitor treatment, actin stress fiber imaging, muscle contraction assay Journal of immunology (Baltimore, Md. : 1950) High 30683702
2020 Rab35 controls myelin growth by forming a complex with myotubularin-related PI 3-phosphatases MTMR13 and MTMR2; Rab35-dependent lipid turnover by myotubularins downregulates PI 3-phosphate levels and mTORC1 activity. Targeted disruption of Rab35 causes elevated PI 3-phosphates, hyperactivation of mTORC1, and focal hypermyelination in vivo, rescued by pharmacological inhibition of PI(3,5)P2 synthesis or mTORC1. Co-IP (complex formation), in vivo Rab35 knockout, PI phospholipid measurement, mTORC1 activity assay, pharmacological rescue Nature communications High 32503983
2020 The C-terminal polybasic cluster (approximately 30 amino acids) of Rab35 is critical for its plasma membrane localization; reducing the number of basic amino acids in this region shifts Rab35 localization from the plasma membrane to the Golgi membrane. Rab35-Rab10 chimera constructs, confocal microscopy of EGFP-fused chimeras Acta histochemica et cytochemica Medium 32873993
2020 Rab35 promotes tunneling nanotube (TNT) formation and TNT-mediated vesicle transfer in neuronal cells; Rab35-GTP, ACAP2, ARF6-GDP, and EHD1 act in a cascade to promote TNT formation, while MICAL-L1 (required for neurite outgrowth) is not required for TNTs, indicating distinct pathway branching. Overexpression of GTP/GDP-locked mutants, dominant-negative expression, TNT quantification, vesicle transfer assay Scientific reports Medium 33033331
2021 BAG3 interacts with TBC1D10B (EPI64B, the Rab35 GAP) via a BAG3-HSP70-TBC1D10B complex that attenuates TBC1D10B's ability to inactivate RAB35; this supports RAB35 activation and HRS recruitment to initiate ESCRT-mediated endosomal tau clearance. BAG3-TBC1D10B colocalization is reduced in AD brains. Mass spectrometry (BAG3 interactome), Co-IP, live-cell imaging, BAG3 overexpression in P301S tau transgenic mice, IHC in human AD brains Biological psychiatry High 35000752
2021 Rab35 negatively regulates Aβ production by sorting APP and BACE1 out of the endosomal network; Rab35 coordinates distinct trafficking steps for BACE1 and APP mediated by its effectors OCRL and ACAP2, respectively. Rab35 overexpression prevents amyloidogenic trafficking of APP and BACE1 induced by high glucocorticoid levels. Overexpression and knockdown, APP/BACE1 localization assays, Aβ production measurement, dominant-negative effector expression Cell death & disease High 34876559
2021 Rab35 regulates insulin secretion in pancreatic β cells and interacts with phogrin (a marker of insulin granule exocytosis); Rab35 overexpression promotes insulin secretion and decreases phogrin expression, while Rab35 silencing inhibits insulin secretion and causes phogrin redistribution. The Rab35/phogrin interaction was confirmed by GST pulldown, co-IP, and co-localization. GST pulldown, co-IP, co-localization microscopy, insulin secretion assay in β-TC-6 cells Clinical and experimental pharmacology & physiology Medium 34448213
2023 PI(4,5)P2 recruits Rab35 to migrasome formation sites by interacting with the C-terminal polybasic cluster of Rab35; active Rab35 then promotes migrasome formation by recruiting and concentrating integrin α5 at these sites, likely via direct Rab35-integrin α5 interaction. Live imaging, PI(4,5)P2 manipulation, Rab35 mutant expression, integrin α5 co-localization and pulldown Cell research High 37142675
2023 DENND2B functions as a Rab35 GEF that recruits and activates Rab35 at the intercellular bridge (ICB) during cytokinesis; DENND2B's N-terminal region also interacts with active Rab35, making it both a GEF and effector. Knockdown of DENND2B delays abscission, causes multinucleation, F-actin accumulation at the ICB, impairs ESCRT-III recruitment, forms chromatin bridges, and activates the Aurora B NoCut/abscission checkpoint. GEF activity assay, Co-IP, siRNA knockdown, cytokinesis assays, ESCRT-III imaging, Aurora B activation measurement Cell reports High 37454296
2023 The Chlamydia trachomatis effector CpoS interacts with Rab35 to suppress STING-dependent type I interferon responses; depletion of Rab35 phenocopies exacerbated interferon responses seen during CpoS-deficient infection, and CpoS mediates transport of sphingolipids to the inclusion via Rab GTPase interaction. Genetic complementation with CpoS variants, Rab35 siRNA knockdown, interferon response measurement, lipid transport assay mBio Medium 37530528
2023 DENND1A and folliculin (FLCN) function as distinct Rab35 GEFs in different cellular contexts: in 3D MDCK cysts, only DENND1A knockout phenocopies Rab35 KO for podocalyxin (PODXL) mis-sorting, while FLCN knockdown phenocopies Rab35 KD in 2D cultures. The FLCN-Rab35-OCRL and DENND1A-Rab35-ACAP2 axes define two distinct functional cascades. siRNA knockdown, CRISPR knockout, PODXL trafficking assays in 2D and 3D MDCK cultures The Journal of biological chemistry High 31992598
2023 USP32, a deubiquitylase, binds Rab35 and protects it from proteasomal degradation by reducing K48-ubiquitination; ETV1 transcription factor promotes USP32 expression in GISTs, and loss of Rab35 decreases exosome secretion and transmission of imatinib resistance. Co-IP, iTRAQ proteomics, ubiquitin assays, knockdown/overexpression, exosome quantification Oncogene High 36725886
2024 A homozygous missense variant R27H in the GTPase fold of RAB35 causes a neurodevelopmental disorder; functional analysis shows R27H-Rab35 has enhanced interaction with its GEF DENND1A and decreased interaction with effector MICAL1, indicating an inactive conformation. R27H expression activates Arf6 (under negative Rab35 control), delays cytokinesis, and alters primary cilia length, number, and Arl13b composition. Exome sequencing, in vitro binding assays (GEF and effector interactions), Arf6 activation assay, cytokinesis assay, primary cilia analysis in patient variant The Journal of biological chemistry High 38432637
2024 The Shigella effector IcsB post-translationally acylates Rab35 in its polybasic region (lysine Nε-fatty acylation), non-canonically entrapping Rab35 at the bacterial-containing vacuole (BCV); Rab35 and IcsB are required not for initial BCV breakage but for unwrapping damaged BCV remnants from Shigella after vacuolar rupture. STED super-resolution microscopy, bacterial genetics (IcsB mutants), post-translational modification analysis, infection assays Cell reports High 38568808
2024 Congenital Rab35 knockout in mice causes embryonic lethality at E7.5; brain-specific Rab35 knockout causes severe defects in hippocampal lamination due to impaired pyramidal neuron distribution, and these mice show spatial memory and anxiety-related behavioral defects. Loss of RAB35 affects other RAB proteins associated with endocytic trafficking and cell adhesion molecules including contactin-2. Rab35 knockout mice (congenital and brain-specific), quantitative proteomics, behavioral testing, histology Communications biology High 37085665
2024 Retromer directly associates with the RAB35 regulator TBC1D13 at the endosomal retrieval sub-domain, revealed by proximity proteomics and X-ray crystallography combined with biochemical validation, indicating Retromer is a hub for RAB35 GTPase cycle regulation at endosomes. Comparative proximity proteomics, X-ray crystallography, in silico predictions, biochemical validation bioRxivpreprint Medium bio_10.1101_2024.11.22.622898
2024 MICAL1, activated by Rab35 at HIV-1 budding sites, locally depolymerizes branched actin networks to promote HIV-1 budding and release; Rab35 is recruited at budding sites and functions in the same pathway as MICAL1, and both are required for viral release. MICAL1 controls timely ESCRT scission machinery recruitment during budding. Super-resolution microscopy, siRNA knockdown, Arp2/3 inhibition rescue, ESCRT recruitment imaging bioRxivpreprint Medium bio_10.1101_2024.10.07.616958

Source papers

Stage 0 corpus · 94 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Rab35 regulates an endocytic recycling pathway essential for the terminal steps of cytokinesis. Current biology : CB 311 16950109
2011 Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis. Nature cell biology 240 21706022
2019 Long non-coding RNA HOTAIR promotes exosome secretion by regulating RAB35 and SNAP23 in hepatocellular carcinoma. Molecular cancer 226 30943982
2010 The Connecdenn DENN domain: a GEF for Rab35 mediating cargo-specific exit from early endosomes. Molecular cell 164 20159556
2008 Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein. The EMBO journal 151 18354496
2012 An ARF6/Rab35 GTPase cascade for endocytic recycling and successful cytokinesis. Current biology : CB 128 22226746
2008 Rab35 and its GAP EPI64C in T cells regulate receptor recycling and immunological synapse formation. The Journal of biological chemistry 125 18450757
2012 Rab35 regulates Arf6 activity through centaurin-β2 (ACAP2) during neurite outgrowth. Journal of cell science 124 22344257
2018 LRRK2 kinase regulates α-synuclein propagation via RAB35 phosphorylation. Nature communications 121 30150626
2009 Rab35 controls actin bundling by recruiting fascin as an effector protein. Science (New York, N.Y.) 119 19729655
2016 Rab35 GTPase: A Central Regulator of Phosphoinositides and F-actin in Endocytic Recycling and Beyond. Traffic (Copenhagen, Denmark) 113 27329675
2012 Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration. Journal of cell science 105 23264734
2016 Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway. The Journal of neuroscience : the official journal of the Society for Neuroscience 100 27535913
2016 Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening. Nature communications 96 27040773
2011 MICAL-L1 is a tubular endosomal membrane hub that connects Rab35 and Arf6 with Rab8a. Traffic (Copenhagen, Denmark) 89 21951725
2009 Rab35 regulates neurite outgrowth and cell shape. FEBS letters 80 19289122
2015 Rab35 GTPase Triggers Switch-like Recruitment of the Lowe Syndrome Lipid Phosphatase OCRL on Newborn Endosomes. Current biology : CB 79 26725203
2017 Rab35 GTPase recruits NDP52 to autophagy targets. The EMBO journal 75 28848034
2010 The connecdenn family, Rab35 guanine nucleotide exchange factors interfacing with the clathrin machinery. The Journal of biological chemistry 73 20154091
2011 Rab35 regulates phagosome formation through recruitment of ACAP2 in macrophages during FcγR-mediated phagocytosis. Journal of cell science 72 22045739
2014 Rab35 promotes the recruitment of Rab8, Rab13 and Rab36 to recycling endosomes through MICAL-L1 during neurite outgrowth. Biology open 71 25086062
2023 The phosphatidylinositol (4,5)-bisphosphate-Rab35 axis regulates migrasome formation. Cell research 70 37142675
2010 Rab35 mediates transport of Cdc42 and Rac1 to the plasma membrane during phagocytosis. Molecular and cellular biology 64 20065041
2011 Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell structure and function 60 21737958
2017 Planar polarized Rab35 functions as an oscillatory ratchet during cell intercalation in the Drosophila epithelium. Nature communications 54 28883443
2013 Rab35 is required for Wnt5a/Dvl2-induced Rac1 activation and cell migration in MCF-7 breast cancer cells. Cellular signalling 50 23353182
2015 Sorting of Clathrin-Independent Cargo Proteins Depends on Rab35 Delivered by Clathrin-Mediated Endocytosis. Traffic (Copenhagen, Denmark) 49 25988331
2010 Recycling of the Ca2+-activated K+ channel, KCa2.3, is dependent upon RME-1, Rab35/EPI64C, and an N-terminal domain. The Journal of biological chemistry 48 20360009
2012 Whacked and Rab35 polarize dynein-motor-complex-dependent seamless tube growth. Nature cell biology 47 22407366
2011 Connecdenn 3/DENND1C binds actin linking Rab35 activation to the actin cytoskeleton. Molecular biology of the cell 47 22072793
2010 Rab35--a vesicular traffic-regulating small GTPase with actin modulating roles. FEBS letters 46 19931531
2012 TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes. Traffic (Copenhagen, Denmark) 40 22762500
2015 Intracellular Uropathogenic E. coli Exploits Host Rab35 for Iron Acquisition and Survival within Urinary Bladder Cells. PLoS pathogens 39 26248231
2019 Rab35 controls cilium length, function and membrane composition. EMBO reports 38 31432619
2015 Activation-Inactivation Cycling of Rab35 and ARF6 Is Required for Phagocytosis of Zymosan in RAW264 Macrophages. Journal of immunology research 37 26229970
2014 Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination. Molecular biology of the cell 37 24600047
2020 Rab35 and its effectors promote formation of tunneling nanotubes in neuronal cells. Scientific reports 35 33033331
2016 Rab35 Functions in Axon Elongation Are Regulated by P53-Related Protein Kinase in a Mechanism That Involves Rab35 Protein Degradation and the Microtubule-Associated Protein 1B. The Journal of neuroscience : the official journal of the Society for Neuroscience 35 27383602
2015 MicroRNA-720 promotes in vitro cell migration by targeting Rab35 expression in cervical cancer cells. Cell & bioscience 35 26413265
2018 Rab35 GTPase and cancer: Linking membrane trafficking to tumorigenesis. Traffic (Copenhagen, Denmark) 34 29314576
2017 Rab35 protein regulates evoked exocytosis of endothelial Weibel-Palade bodies. The Journal of biological chemistry 31 28566286
2016 Increased Rab35 expression is a potential biomarker and implicated in the pathogenesis of Parkinson's disease. Oncotarget 31 27509057
2022 Transcellular propagation of fibrillar α-synuclein from enteroendocrine to neuronal cells requires cell-to-cell contact and is Rab35-dependent. Scientific reports 28 35264710
2017 Coordination of synaptic vesicle trafficking and turnover by the Rab35 signaling network. Small GTPases 28 28129039
2020 Rab35-regulated lipid turnover by myotubularins represses mTORC1 activity and controls myelin growth. Nature communications 26 32503983
2018 The small GTPase RAB-35 defines a third pathway that is required for the recognition and degradation of apoptotic cells. PLoS genetics 26 30138370
2016 EGF-stimulated activation of Rab35 regulates RUSC2-GIT2 complex formation to stabilize GIT2 during directional lung cancer cell migration. Cancer letters 26 27238570
2016 Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation. Histochemistry and cell biology 25 26791531
2012 Rab35 regulates cadherin-mediated adherens junction formation and myoblast fusion. Molecular biology of the cell 25 23197472
2021 BAG3 Regulation of RAB35 Mediates the Endosomal Sorting Complexes Required for Transport/Endolysosome Pathway and Tau Clearance. Biological psychiatry 23 35000752
2018 A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration. Nature communications 23 29662076
2018 Rab35-dependent extracellular nanovesicles are required for induction of tumour supporting stroma. Nanoscale 22 29693684
2023 The Chlamydia effector CpoS modulates the inclusion microenvironment and restricts the interferon response by acting on Rab35. mBio 21 37530528
2021 MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells. Aging 21 34500436
2018 RAB-35 and ARF-6 GTPases Mediate Engulfment and Clearance Following Linker Cell-Type Death. Developmental cell 21 30220571
2021 Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production. Cell death & disease 20 34876559
2019 RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis. Molecular human reproduction 20 31152166
2023 Deubiquitylation of Rab35 by USP32 promotes the transmission of imatinib resistance by enhancing exosome secretion in gastrointestinal stromal tumours. Oncogene 19 36725886
2017 Folliculin Interacts with Rab35 to Regulate EGF-Induced EGFR Degradation. Frontiers in pharmacology 18 29018350
2016 Enterohaemorrhagic E. coli modulates an ARF6:Rab35 signaling axis to prevent recycling endosome maturation during infection. Journal of molecular biology 18 27261256
2012 Trafficking cascades mediated by Rab35 and its membrane hub effector, MICAL-L1. Communicative & integrative biology 17 23060965
2019 Combined Assessment of Serum Alpha-Synuclein and Rab35 is a Better Biomarker for Parkinson's Disease. Journal of clinical neurology (Seoul, Korea) 16 31591837
2019 IL-17A Recruits Rab35 to IL-17R to Mediate PKCα-Dependent Stress Fiber Formation and Airway Smooth Muscle Contractility. Journal of immunology (Baltimore, Md. : 1950) 15 30683702
2015 Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-β2/ACAP2 during neurite outgrowth of PC12 cells. The Journal of biological chemistry 15 25694427
2018 EGF Stimulates Rab35 Activation and Gastric Cancer Cell Migration by Regulating DENND1A-Grb2 Complex Formation. Frontiers in pharmacology 14 30524285
2021 Rab35 regulates skeletogenesis and gastrulation by facilitating actin remodeling and vesicular trafficking. Cells & development 12 33842922
2015 Measurement of Rab35 activity with the GTP-Rab35 trapper RBD35. Methods in molecular biology (Clifton, N.J.) 12 25800845
2021 The dual functions of Rab11 and Rab35 GTPases-regulation of cell division and promotion of tumorigenicity. American journal of cancer research 9 34094658
2020 Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures. The Journal of biological chemistry 9 31992598
2023 RAB35 is required for murine hippocampal development and functions by regulating neuronal cell distribution. Communications biology 8 37085665
2023 DENND2B activates Rab35 at the intercellular bridge, regulating cytokinetic abscission and tetraploidy. Cell reports 8 37454296
2021 miR-720 Regulates Insulin Secretion by Targeting Rab35. BioMed research international 8 33880375
2022 TRX2/Rab35 Interaction Impairs Exosome Secretion by Inducing Rab35 Degradation. International journal of molecular sciences 7 35743001
2021 Proteomic analysis reveals brain Rab35 as a potential biomarker of mitragynine withdrawal in rats. Brain research bulletin 7 33901587
2019 The small GTPase RAB-35 facilitates the initiation of phagosome maturation and acts as a robustness factor for apoptotic cell clearance. Small GTPases 6 31607221
2023 A Rab39-Klp98A-Rab35 endocytic recycling pathway is essential for rapid Golgi-dependent furrow ingression. Development (Cambridge, England) 3 37590130
2024 Rab35 Is Required for Embryonic Development and Kidney and Ureter Homeostasis through Regulation of Epithelial Cell Junctions. Journal of the American Society of Nephrology : JASN 2 38530365
2024 Coxiella burnetii-containing vacuoles interact with host recycling endosomal proteins Rab11a and Rab35 for vacuolar expansion and bacterial growth. Frontiers in cellular and infection microbiology 2 38841112
2024 Role of Rab35 in modulating lipid metabolism and viral entry during pseudorabies virus infection. International journal of biological macromolecules 2 39528177
2022 Stratum is required for both apical and basolateral transport through stable expression of Rab10 and Rab35 in Drosophila photoreceptors. Molecular biology of the cell 2 35767331
2020 Rab35 Targeting to the Plasma Membrane Is Dependent on the C-terminal Polybasic Cluster. Acta histochemica et cytochemica 2 32873993
2024 A neurodevelopmental disorder associated with a loss-of-function missense mutation in RAB35. The Journal of biological chemistry 1 38432637
2024 Post-translational targeting of Rab35 by the effector IcsB of Shigella determines intracellular bacterial niche formation. Cell reports 1 38568808
2024 New aspects of a small GTPase RAB35 in brain development and function. Neural regeneration research 1 39254551
2022 Rab35 GTPase positively regulates endocytic recycling of cardiac KATP channels. Channels (Austin, Tex.) 1 35754325
2021 Rab35 regulates insulin secretion via phogrin in pancreatic β cells. Clinical and experimental pharmacology & physiology 1 34448213
2026 RAB-35 regulates distinct steps of trogocytosis in the biting and bitten cell. bioRxiv : the preprint server for biology 0 41756979
2026 Rab35 drives the malignant progression of endometrial carcinoma by regulating the nuclear translocation of β-catenin. Experimental cell research 0 41763512
2025 A conserved Arf-GEF modulates axonal integrity through RAB-35 by altering neuron-epidermal attachment. bioRxiv : the preprint server for biology 0 40661396
2025 The unique contributions of Rab11 and Rab35 to the completion of cell division. Biological research 0 40877912
2025 Linc01271 promotes lipid synthesis and MASLD/MASH progression via miR-149-3p/RAB35 axis. Cellular and molecular life sciences : CMLS 0 40920256
2023 Rab35 is required for embryonic development and kidney and ureter homeostasis through regulation of epithelial cell junctions. bioRxiv : the preprint server for biology 0 37745459
2021 Functional variant rs17525453 within RAB35 gene promoter is possibly associated with increased risk of Parkinson's disease in Taiwanese population. Neurobiology of aging 0 34275689
2021 Rab35 controls formation of luminal projections required for bile canalicular morphogenesis. The Journal of cell biology 0 34515738