Affinage

RAB35

Ras-related protein Rab-35 · UniProt Q15286

Length
201 aa
Mass
23.0 kDa
Annotated
2026-06-10
95 papers in source corpus 51 papers cited in narrative 52 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAB35 is a small GTPase that operates as a master regulator of fast endocytic recycling and plasma-membrane-proximal membrane dynamics, coordinating cytokinesis, cell polarity, ciliogenesis, actin remodeling, and selective autophagy (PMID:16950109, PMID:27040773, PMID:31432619). Its activity is set by a GTPase switch controlled by multiple DENN-domain GEFs (DENND1A/B/C, DENND2B) and folliculin that activate RAB35 at clathrin-coated structures, recycling endosomes, and the cytokinetic bridge (PMID:20159556, PMID:20154091, PMID:37454296, PMID:31992598), opposed by a panel of TBC-domain GAPs (EPI64B/TBC1D10B, EPI64C/TBC1D10C, TBC1D13, TBC1D10A) (PMID:18450757, PMID:22226746, PMID:22762500, PMID:28566286); plasma-membrane and migrasome targeting depends on its C-terminal polybasic cluster, which reads PI(4,5)P2 (PMID:32873993, PMID:37142675). A central output of active RAB35 is suppression of Arf6, achieved by recruiting the Arf6-GAP ACAP2/centaurin-β2, which links RAB35 to actin remodeling, neurite outgrowth, cadherin-based adhesion, and inhibition of migration (PMID:22344257, PMID:23264734, PMID:25694427). In parallel RAB35 recruits the inositol 5-phosphatase OCRL to newborn endocytic vesicles and the cytokinetic intercellular bridge to hydrolyze PI(4,5)P2, controlling F-actin disassembly and abscission, while DENND2B-driven RAB35 activation enables ESCRT-III recruitment for bridge resolution (PMID:21706022, PMID:26725203, PMID:37454296). RAB35 effectors also include the actin-bundler fascin (PMID:19729655), MICAL-L1 scaffolding of downstream Rabs (PMID:21951725, PMID:25086062), the ESCRT-0 component Hrs and the autophagy receptor NDP52, the latter directing antibacterial autophagy and mitophagy (PMID:27535913, PMID:28848034), and the myotubularin phosphatases MTMR2/MTMR13, through which RAB35 limits PI(3)P levels and mTORC1 to control myelin growth (PMID:32503983). RAB35 establishes apico-basal polarity by tethering Podocalyxin-containing vesicles during the first division of epithelial cysts (PMID:27040773), and its activity is regulated by LRRK2 phosphorylation, linking it to α-synuclein propagation (PMID:30150626). Loss of RAB35 is embryonic-lethal in mice and causes hippocampal lamination and renal (hydronephrosis) defects (PMID:37085665, PMID:38530365), and a homozygous R27H GTPase-fold variant that locks RAB35 in an inactive conformation underlies a human neurodevelopmental disorder (PMID:38432637).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2006 High

    Established RAB35 as the GTPase governing a fast endocytic recycling route and revealed an unexpected requirement in cytokinesis, defining its core cell-biological remit.

    Evidence Dominant-negative and RNAi perturbation with live imaging and endocytic-marker assays in dividing cells

    PMID:16950109

    Open questions at the time
    • GEFs/GAPs setting RAB35 activity unidentified at this stage
    • molecular effectors mediating recycling and abscission not yet known
  2. 2008 High

    Identified the first GEF (RME-4) and a specific GAP (EPI64C/TBC1D10C), placing RAB35 within the clathrin pathway and showing the GTPase cycle controls receptor recycling and immune-cell function.

    Evidence C. elegans genetic epistasis and Y2H binding; in vitro GAP activity assay and T cell conjugate/immunological synapse assays

    PMID:18354496 PMID:18450757

    Open questions at the time
    • mammalian GEF identity not yet established
    • downstream effectors of recycling unresolved
  3. 2009 High

    Connected RAB35 to actin by identifying fascin as a direct GTP-dependent effector and a Cdc42-dependent role in neurite outgrowth, broadening RAB35 from trafficking into cytoskeletal control.

    Evidence Drosophila genetics, active-RAB35 pulldown of fascin, mitochondrial ectopic-targeting reconstitution; siRNA and Cdc42 activation assays in neuronal cell lines

    PMID:19289122 PMID:19729655

    Open questions at the time
    • how RAB35 links to Cdc42 activation mechanistically unclear
    • relationship between fascin bundling and recycling roles undefined
  4. 2010 High

    Defined the connecdenn/DENND1 family as mammalian RAB35 GEFs coupled to clathrin/AP-2, explaining how RAB35 activation is spatially tied to endocytosis and recycling.

    Evidence In vitro GEF assays for DENND1A/B/C, Co-IP/Y2H with clathrin and AP-2, siRNA endosomal-morphology and receptor recycling readouts

    PMID:20154091 PMID:20159556

    Open questions at the time
    • division of labor among the three GEF paralogs not resolved
    • structural basis of GEF specificity unknown
  5. 2011 High

    Resolved the abscission mechanism by showing active RAB35 recruits the PI(4,5)P2 5-phosphatase OCRL to the intercellular bridge to clear PI(4,5)P2 and F-actin, and catalogued additional effectors (MICAL-L1, RUSC2, ACAP2 in phagocytosis).

    Evidence GTP-RAB35 pulldown/Co-IP with OCRL, patient cells, Y2H RUN-domain screen, and RNAi/live imaging in macrophages

    PMID:21706022 PMID:21737958 PMID:21951725 PMID:22045739 PMID:22072793

    Open questions at the time
    • temporal ordering of OCRL recruitment not yet defined
    • how multiple effectors are coordinated on one GTPase unclear
  6. 2012 High

    Built the RAB35–Arf6 regulatory axis: active RAB35 recruits ACAP2 to inactivate Arf6 driving neurite outgrowth, adhesion, and migration control, while Arf6→EPI64B feedback and additional GAPs (TBC1D13) place RAB35 in defined hierarchies and the GLUT4 pathway.

    Evidence Knockdown-rescue with specific mutants, epistasis, in vitro GAP screen, and cargo/GLUT4 trafficking assays

    PMID:22226746 PMID:22344257 PMID:22407366 PMID:22762500 PMID:23197472 PMID:23264734

    Open questions at the time
    • precise residues mediating effector selection not yet mapped
    • how cadherin vs integrin sorting is partitioned mechanistically unresolved
  7. 2014 Medium

    Positioned RAB35 as a master Rab upstream of a MICAL-L1 scaffold that recruits Rab8/Rab13/Rab36, and showed RAB35–ACAP2 negatively regulates oligodendrocyte differentiation and myelination.

    Evidence siRNA knockdown-rescue with non-redundant downstream Rab tests; GTPase activity assays in oligodendrocyte cocultures

    PMID:24600047 PMID:25086062

    Open questions at the time
    • direct vs indirect recruitment of downstream Rabs not distinguished
    • in vivo relevance of myelination role not yet shown at this stage
  8. 2015 High

    Established real-time temporal logic of the GTPase cycle on nascent vesicles and mapped specific switch-II residues governing effector selection, while extending cargo scope to CIE proteins and ion channels.

    Evidence Live-imaging of timed DENND1A/EPI64B/RAB35/OCRL events, site-directed mutagenesis of T76/T81, and trafficking/flow-cytometry assays

    PMID:20360009 PMID:25694427 PMID:25988331 PMID:26725203

    Open questions at the time
    • structural mechanism of differential effector binding incomplete
    • generality of timed cascade across cargo types untested
  9. 2016 High

    Linked RAB35 to apico-basal polarity initiation via direct Podocalyxin tethering, to synaptic vesicle protein degradation via Hrs/ESCRT, and to ciliogenesis, and identified PRPK-mediated RAB35 turnover regulated by MAP1B.

    Evidence 3D MDCK cyst culture with RAB35-Podocalyxin pulldown; Co-IP of RAB35-Hrs and activity assays in neurons; GFP localization and Co-IP in cilia; proteasome-inhibitor rescue

    PMID:27040773 PMID:27383602 PMID:27535913 PMID:31432619

    Open questions at the time
    • how cytokinesis is coupled to polarity initiation mechanistically incompletely defined
    • ciliary effector hierarchy not fully resolved
  10. 2017 High

    Extended RAB35 effector logic to selective autophagy (direct NDP52 recruitment for xenophagy and mitophagy), regulated WPB exocytosis, and epithelial morphogenesis as a planar-polarized ratchet.

    Evidence Active-RAB35 pulldown of NDP52 and TBK1 linkage; genome-wide RabGAP screen with rescue for WPB; Drosophila live imaging of intercalation

    PMID:28566286 PMID:28848034 PMID:28883443

    Open questions at the time
    • upstream signal triggering RAB35 activation in autophagy partly undefined
    • structural basis of NDP52 vs other effector binding unknown
  11. 2018 High

    Connected RAB35 to neurodegeneration via LRRK2 phosphorylation driving α-synuclein propagation, and defined roles in chemotactic migration (PI3K/p85), TNT formation, and conserved phagocytic clearance pathways.

    Evidence Multi-organism LRRK2 inhibitor and constitutively active RAB35 rescue; RNAi/PI3K assays; C. elegans genetics and Co-IP of RAB-35 with CNT-1

    PMID:23264734 PMID:29662076 PMID:30138370 PMID:30150626 PMID:30220571

    Open questions at the time
    • functional consequence of RAB35 phosphorylation on its GTPase cycle not fully resolved
    • how PI3K activity is directly regulated by RAB35 unclear
  12. 2020 High

    Defined an in vivo myelin-growth-control mechanism whereby RAB35 complexes with MTMR2/MTMR13 to limit PI(3)P and mTORC1, and dissected RAB35's polybasic membrane-targeting determinant.

    Evidence Co-IP, conditional KO mice with phosphoinositide and mTORC1 readouts and pharmacological rescue; RAB35-Rab10 chimera localization

    PMID:32503983 PMID:32873993 PMID:33033331

    Open questions at the time
    • tissue specificity of MTMR complex vs other effectors unresolved
    • how membrane targeting integrates with effector choice unclear
  13. 2021 Medium

    Implicated RAB35 in proteostasis and Alzheimer-relevant trafficking, showing BAG3/HSP70 stabilizes active RAB35 for ESCRT-mediated tau clearance and that RAB35 sorts APP/BACE1 to limit Aβ via distinct OCRL and ACAP2 cascades.

    Evidence Mass spectrometry, Co-IP, AD brain/tau-mouse models; effector-specific rescue with Aβ measurements

    PMID:34876559 PMID:35000752

    Open questions at the time
    • causal contribution to human disease not established from cell models alone
    • how single RAB35 directs two cargos via different effectors mechanistically unresolved
  14. 2023 High

    Identified new regulatory inputs and outputs: PIP5K1A/PI(4,5)P2-driven RAB35 recruitment with direct integrin-α5 capture for migrasome biogenesis, DENND2B as the cytokinetic-bridge GEF, USP32 deubiquitination stabilizing RAB35, a Shigella acyltransferase that traps RAB35, and dual GEF cascades (FLCN vs DENND1A) for Podocalyxin.

    Evidence Sequential interaction/Co-IP assays, migrasome and abscission readouts, K48-ubiquitination assays, STED imaging of IcsB acylation, and CRISPR KO with GEF-domain rescue

    PMID:31992598 PMID:36725886 PMID:37142675 PMID:37454296 PMID:38568808

    Open questions at the time
    • how distinct GEFs select context-specific effector cascades unresolved
    • stoichiometry of RAB35 with integrin-α5 at migrasomes unknown
  15. 2024 High

    Cemented physiological and pathological importance through a human inactivating R27H variant causing neurodevelopmental disease, in vivo KO mice revealing embryonic lethality and brain/kidney developmental defects, and a host-pathway role in HIV-1 budding via MICAL1.

    Evidence Exome sequencing with Co-IP/Arf6/cilia readouts; conditional KO mice with histology and proteomics; siRNA/super-resolution viral-release assays (preprint for HIV-1)

    PMID:37085665 PMID:38432637 PMID:38530365

    Open questions at the time
    • genotype-phenotype spectrum of human RAB35 variants undefined
    • tissue-specific effector dependencies underlying KO phenotypes not fully dissected

Open questions

Synthesis pass · forward-looking unresolved questions
  • How a single GTPase selects among its many GEFs, GAPs, and competing effectors to execute context-specific outputs—and the structural and post-translational logic governing this selectivity—remains unresolved.
  • no unifying structural model of effector discrimination
  • post-translational modification (phosphorylation, ubiquitination, acylation) integration into the GTPase cycle incompletely mapped
  • tissue-level effector hierarchies underlying in vivo phenotypes undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003924 GTPase activity 4 GO:0098772 molecular function regulator activity 4 GO:0060089 molecular transducer activity 3
Localization
GO:0005768 endosome 4 GO:0005886 plasma membrane 3 GO:0005929 cilium 2 GO:0005794 Golgi apparatus 1
Pathway
R-HSA-1266738 Developmental Biology 3 R-HSA-162582 Signal Transduction 3 R-HSA-1640170 Cell Cycle 3 R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-9612973 Autophagy 2
Partners
ACAP2ARL13BDENND1ADENND2BMICAL-L1NDP52OCRLPODOCALYXIN
Complex memberships
BAG3-HSP70-TBC1D10B complexRAB35-MTMR2/MTMR13 phosphatase complex

Evidence

Reading pass · 52 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2006 Rab35 localizes to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway; inhibition of Rab35 leads to cytokinesis failure, and Rab35 is required for intercellular bridge localization of PIP2 and the septin SEPT2 during cytokinesis. Dominant-negative overexpression, RNAi knockdown, fluorescence microscopy, endocytic marker accumulation assay in dividing cells Current biology : CB High 16950109
2008 In C. elegans, the DENN-domain protein RME-4 functions as a GEF activator of RAB-35 (Rab35 ortholog); RME-4 binds RAB-35 in its GDP-loaded conformation, localizes to clathrin-coated pits, and recruits RAB-35 to endosomes for receptor recycling. Genetic epistasis places RAB-35 downstream of clathrin, upstream of RAB-7, and synergistically with recycling regulators RAB-11 and RME-1. C. elegans genetic screen, yeast two-hybrid binding assay, epistasis analysis, GFP localization, RNAi The EMBO journal High 18354496
2008 EPI64C (TBC1D10C) is a Rab35-specific GAP (demonstrated by in vitro GAP activity assay); both EPI64C overexpression and Rab35 dominant-negative impair transferrin recycling in T cells and inhibit immunological synapse formation. Rab35 is recruited to the immunological synapse and regulates TCR transport there. In vitro GAP activity assay, dominant-negative transfection, immunofluorescence, T cell conjugate assay, mass spectrometry identification The Journal of biological chemistry High 18450757
2009 Rab35 regulates actin filament assembly during Drosophila bristle development and filopodia formation; the actin-bundling protein fascin directly associates with GTP-bound (active) Rab35 as an effector. Targeting Rab35 to the outer mitochondrial membrane is sufficient to trigger actin recruitment. Drosophila genetics, pulldown of fascin with active Rab35, mitochondrial targeting assay, cell culture filopodia assay Science (New York, N.Y.) High 19729655
2009 Rab35 colocalizes with actin filaments and with Cdc42; active Rab35 stimulates neurite outgrowth in PC12 and N1E-115 cells via a Cdc42-dependent pathway, and siRNA knockdown of Rab35 abolishes neurite outgrowth. siRNA knockdown, dominant-active/dominant-negative transfection, Cdc42 GEF activity assay in vivo and in vitro, fluorescence colocalization FEBS letters Medium 19289122
2010 The DENN domain of connecdenn/DENND1A functions as a guanine nucleotide exchange factor (GEF) for Rab35, activating it to regulate endosomal membrane trafficking; loss of Rab35 activity enlarges early endosomes, inhibits MHC class I recycling, and prevents EHD1 recruitment to endosomal tubules. In vitro GEF activity assay, dominant-negative/siRNA knockdown, endosomal morphology assay, receptor recycling assay Molecular cell High 20159556
2010 All three connecdenn family members (DENND1A, DENND1B, DENND1C) function as GEFs for Rab35 through their DENN domains; they interact with clathrin and AP-2, linking Rab35 activation to the clathrin machinery. Connecdenn 2 knockdown enlarges early endosomes similarly to Rab35 loss. In vitro GEF activity assay, Co-IP, siRNA knockdown, yeast two-hybrid, endosomal morphology The Journal of biological chemistry High 20154091
2010 Rab35 mediates transport of Cdc42 and Rac1 to the plasma membrane during phagocytosis in Drosophila immune cells; recruitment of these Rho GTPases to filopodium and lamellipodium formation sites is Rab35-dependent and occurs via microtubule tracks. Drosophila genetic screen, fluorescence microscopy, RNAi knockdown, microtubule disruption assay Molecular and cellular biology Medium 20065041
2011 Active GTP-bound Rab35 directly interacts with the PI(4,5)P2 5-phosphatase OCRL, which is an effector of Rab35; Rab35 controls OCRL localization at the intercellular bridge during cytokinesis. Depletion of either Rab35 or OCRL causes abnormal PI(4,5)P2 and F-actin accumulation at the intercellular bridge, inhibiting abscission. Pulldown/Co-IP of GTP-Rab35 with OCRL, siRNA knockdown, live-cell imaging, F-actin/PIP2 staining, patient-derived cell lines Nature cell biology High 21706022
2011 MICAL-L1 is a Rab35 effector on tubular recycling endosomes; overexpression of active Rab35 impairs MICAL-L1 recruitment to tubular recycling endosomes, while Rab35 depletion promotes enhanced MICAL-L1 localization there. Arf6 also forms a complex with MICAL-L1, and Rab35 acts as an upstream regulator of both MICAL-L1 and Arf6 to control Rab8a function. Co-IP, siRNA knockdown, overexpression, fluorescence colocalization Traffic (Copenhagen, Denmark) Medium 21951725
2011 Rab35 regulates phagosome formation during FcγR-mediated phagocytosis in macrophages by recruiting ACAP2 (an ARF6 GAP) to the phagocytic cup in a GTP-Rab35-dependent manner; GTP-Rab35-dependent ACAP2 recruitment controls actin-mediated pseudopod extension. RNAi knockdown, dominant-negative/GTP-locked mutant expression, live-cell imaging, co-localization Journal of cell science Medium 22045739
2011 Connecdenn 3 (DENND1C) directly binds actin via a unique C-terminal motif and couples Rab35 activation to the actin cytoskeleton, producing membrane extensions. Connecdenn 3 colocalizes with fascin and actin filaments, identifying it as the relevant GEF for Rab35's actin-bundling activity. Direct actin-binding assay, Rab35 GEF assay, Co-IP, fluorescence colocalization, cell morphology assay Molecular biology of the cell Medium 22072793
2011 Genome-wide RUN domain screen identified RUSC2 as a GTP-Rab35-specific binding protein; the Rab35-binding domain (RBD35, residues 982-1199 of RUSC2) specifically traps active Rab35 but not other Rabs, and RBD35 overexpression in PC12 cells inhibits NGF-dependent neurite outgrowth. Yeast two-hybrid genome-wide screen, in vitro pulldown, cell overexpression assay Cell structure and function Medium 21737958
2012 ARF6 GTPase negatively regulates Rab35 activation through EPI64B, a Rab35 GAP that is an ARF6 effector; constitutively active ARF6 reduces Rab35 loading into the endocytic pathway at clathrin-coated pits, establishing a hierarchical ARF6→EPI64B→Rab35 cascade controlling endocytic recycling and cytokinesis. Dominant mutant expression, epistasis analysis, fluorescence microscopy, GFP-ARF6 and Rab35 colocalization Current biology : CB High 22226746
2012 Rab35 functions as an effector of Rab35 → centaurin-β2 (ACAP2) → ARF6-GAP cascade during neurite outgrowth: Rab35 accumulates at Arf6-positive endosomes upon NGF stimulation and recruits centaurin-β2 (ACAP2), whose ARF6-GAP activity is required for neurite outgrowth of PC12 cells. siRNA knockdown and rescue experiments, fluorescence colocalization, dominant mutant expression, NGF stimulation assay Journal of cell science High 22344257
2012 Rab35 maintains cadherins at the cell surface to promote cell-cell adhesion and suppresses Arf6 activity, thereby downregulating Arf6-dependent recycling of β1-integrin and EGF receptors to inhibit cell migration; this coordinates inverse intracellular sorting of cadherin and integrin. siRNA knockdown, fluorescence microscopy, receptor trafficking assays, cell migration/adhesion assays Journal of cell science Medium 23264734
2012 In Drosophila tracheal terminal cells, Rab35 and its apical membrane-localized GAP Whacked polarize seamless tube growth along the proximodistal axis; constitutive Rab35 activation (or Whacked loss) causes tube overgrowth at tips, while dominant-negative Rab35 (or Whacked overexpression) causes ectopic tubes. Dynein motor complex is also required for seamless tube growth. Drosophila genetics, loss-of-function and gain-of-function mutants, live imaging Nature cell biology High 22407366
2012 Rab35 regulates cadherin-mediated adherens junction formation: Rab35 accumulates at cell-cell contacts in a cadherin-dependent manner; its knockdown impairs N- and M-cadherin recruitment and their association with p120 catenin, and Rab35 function is required for PIP5KIγ accumulation and PI(4,5)P2 production at cell-cell contacts. Rab35 knockdown also blocks myoblast fusion. siRNA knockdown, dominant-negative expression, Co-IP, fluorescence microscopy, biochemical fractionation Molecular biology of the cell Medium 23197472
2012 TBC1D13 is a Rab35-specific GAP (demonstrated in comprehensive in vitro screen); TBC1D13 displays in vivo GAP activity toward Rab35 and inhibits insulin-stimulated GLUT4 translocation in adipocytes. Constitutively active Rab35 (but not Rab10) reverses this block, placing Rab35 in the insulin-stimulated GLUT4 pathway. In vitro GAP activity screen, in vivo GAP activity assay, constitutively active rescue, GLUT4 translocation assay Traffic (Copenhagen, Denmark) High 22762500
2014 Rab35 promotes NGF-induced neurite outgrowth by recruiting MICAL-L1 to Arf6-positive recycling endosomes, which in turn recruits Rab8, Rab13, and Rab36; Rab35 acts as a master Rab upstream of MICAL-L1-dependent scaffolding of downstream Rabs. Knockdown-rescue experiments confirmed non-redundant roles of each downstream Rab. siRNA knockdown and rescue experiments, fluorescence colocalization, NGF stimulation assay Biology open Medium 25086062
2014 Rab35 and its effector ACAP2 (ARF6 GAP) negatively regulate oligodendrocyte differentiation and myelination by inactivating Arf6; during differentiation, Rab35 and ACAP2 activities decrease while Arf6 activity increases. Knockdown of Rab35 or ACAP2 promotes differentiation, while Arf6 knockdown inhibits it. siRNA knockdown, GTPase activity assays, oligodendrocyte-neuron cocultures, myelination assay Molecular biology of the cell Medium 24600047
2015 Rab35 GTPase acts as a precise spatial and temporal switch for OCRL recruitment on newborn clathrin-coated vesicles immediately after scission from the plasma membrane; Rab35 loading on CCVs follows DENND1A recruitment and EPI64B disappearance. Depletion of Rab35 or OCRL retains internalized receptors (CI-MPR) in peripheral clathrin-positive endosomes with abnormal PI(4,5)P2 and actin. Live-cell imaging, siRNA knockdown, fluorescence microscopy with timed events, receptor trafficking assay Current biology : CB High 26725203
2015 Two threonine residues (Thr-76 and Thr-81) in the switch II region of Rab35 are critical for binding ACAP2/centaurin-β2; two asparagine residues (Asn-610 and Asn-691) of centaurin-β2 are key for specific Rab35 recognition. Neither a centaurin-β2-binding-deficient Rab35(T76S/T81A) mutant nor a Rab35-binding-deficient centaurin-β2(N610A/N691A) mutant can support neurite outgrowth. Deletion and site-directed mutagenesis, pulldown interaction assay, knockdown-rescue functional assay The Journal of biological chemistry High 25694427
2015 Rab35 delivered by clathrin-mediated endocytosis is required for sorting of clathrin-independent endocytosis (CIE) cargo proteins (CD44, CD98, CD147) into Arf6-associated tubules; Rab35 (or its effector ACAPs) inhibits Arf6-GTP to prevent lysosomal degradation of CIE cargo. Rab35 siRNA knockdown elevates Arf6-GTP levels and mimics the phenotype of CME inhibition. CME inhibition, siRNA knockdown, constitutively active Rab35 expression, GTPase activity assay, flow cytometry Traffic (Copenhagen, Denmark) Medium 25988331
2015 Recycling of the KCa2.3 channel depends on Rab35; dominant-negative Rab35 or EPI64C (Rab35 GAP) overexpression traps KCa2.3 in an intracellular compartment. Co-IP confirmed association between KCa2.3 and Rab35. EPI64C's effect was dependent on its GAP activity. Dominant-negative expression, Co-IP, surface biotinylation, EPI64C knockdown, fluorescence microscopy The Journal of biological chemistry Medium 20360009
2016 Rab35 plays a crucial role in polarity initiation and apical lumen positioning during the first cell division of MDCK cyst development; Rab35 physically couples cytokinesis with apico-basal polarity initiation by tethering vesicles containing aPKC, Cdc42, Crumbs3, and Podocalyxin through a direct interaction between Rab35 and the cytoplasmic tail of Podocalyxin. Rab35 inactivation leads to complete polarity inversion. 3D MDCK cyst culture, dominant-negative/constitutively active mutants, pulldown/Co-IP for Rab35-Podocalyxin interaction, fluorescence microscopy Nature communications High 27040773
2016 Neuronal activity drives Rab35 activation; activated Rab35 binds the ESCRT-0 protein Hrs (a novel Rab35 effector), recruiting the downstream ESCRT machinery to synaptic vesicle pools to initiate activity-dependent SV protein degradation. Rab35 activation assay in neurons, Co-IP of Rab35-Hrs, siRNA knockdown, immunofluorescence, degradation assay The Journal of neuroscience Medium 27535913
2016 PRPK (p53-related protein kinase) negatively regulates axon elongation by promoting Rab35 protein degradation via the ubiquitin-proteasome pathway; MAP1B interacts with PRPK to prevent this degradation and thereby allows Rab35-mediated axonal elongation. Rab35 also regulates Cdc42 activity in neurons. MAP1B Co-IP with PRPK, proteasome inhibitor rescue, Rab35 overexpression rescue in MAP1B KO neurons, epistasis The Journal of neuroscience Medium 27383602
2016 Rab35 controls cilium length and membrane composition; GFP-Rab35 localizes to cilia, and Rab35 loss (siRNA, morpholinos, knockout) reduces cilium length in mammalian cells and zebrafish. Rab35 controls ciliary levels of Smoothened, Arl13b, and INPP5E, and interacts with Arl13b. GEF (DENND1B) and GAP (TBC1D10A) for Rab35 also localize to cilia and regulate ciliary length. siRNA knockdown, morpholino in zebrafish, KO cells, GFP localization, immunofluorescence, Co-IP EMBO reports High 31432619
2017 Active GTP-bound Rab35 accumulates on bacteria-containing endosomes and directly binds and recruits the autophagy receptor NDP52 to internalized bacteria; Rab35 also promotes NDP52 interaction with ubiquitin. Rab35 regulates NDP52 recruitment to damaged mitochondria and autophagosomes for mitophagy and autophagosome maturation; TBC1D10A (GAP for Rab35) inhibits and TBK1 stimulates this process. Active Rab35 pulldown with NDP52, Co-IP, Rab35 knockdown, GFP-Rab35 localization, mitophagy assay The EMBO journal High 28848034
2017 Rab35 is dynamically planar polarized in Drosophila epithelial intercalation; Rab35 compartments are enriched at contractile interfaces and act as a ratchet ensuring unidirectional interface shortening. Actomyosin disruption causes Rab35 compartments to fail to terminate, while loss of Rab35 allows contraction but prevents ratcheting. Mesoderm invagination also fails when Rab35 is compromised. Live imaging in Drosophila embryo, genetic loss-of-function, Myosin II disruption, quantitative interface analysis Nature communications High 28883443
2017 Rab35 regulates evoked exocytosis of Weibel-Palade bodies (WPB) in endothelial cells; TBC1D10A is a Rab35 GAP in these cells, and Rab35(Q67A) (GAP-insensitive mutant) rescues TBC1D10A-mediated inhibition. ACAP2 is a Rab35 effector in this context (Co-IP/pulldown), acting as a negative regulator of WPB exocytosis upstream of Arf6. Genome-wide RabGAP screen, pulldown/Co-IP of Rab35-ACAP2, Rab35 siRNA knockdown, dominant-negative Rab35, WPB exocytosis assay The Journal of biological chemistry High 28566286
2018 LRRK2 kinase phosphorylates RAB35, and this phosphorylation mediates LRRK2's role in α-synuclein propagation; the PD-linked G2019S LRRK2 mutation (increased kinase activity) enhances propagation. Constitutive activation of RAB35 overrides reduced α-synuclein propagation in lrk-1 mutant C. elegans. LRRK2 kinase inhibitor reduced α-synuclein aggregation via enhanced lysosomal degradation. Cell culture, C. elegans genetics, rodent models; LRRK2 kinase inhibitor treatment, constitutively active RAB35 rescue, phosphorylation assay Nature communications High 30150626
2018 Rab35 maintains cadherins at cell surface and suppresses Arf6 to coordinate cell adhesion vs. migration; conversely, Rab35 knockdown promotes EMT-like behavior. RAB35 expression is suppressed in cancers characterized by Arf6 hyperactivity. siRNA knockdown, receptor trafficking assay, Arf6 activity assay, cancer cell migration assay Journal of cell science Medium 23264734
2018 RAB35-p85/PI3K axis controls oscillatory apical protrusions (CDRs) required for chemotactic migration; RAB35 is necessary and sufficient for CDR waves and directly regulates p85/PI3K activity polarity to guide directional chemotaxis and chemoinvasion. RNAi screen, live imaging, PI3K activity assay, chemotaxis assay, RAB35 overexpression Nature communications Medium 29662076
2018 In C. elegans, RAB-35 binds CNT-1 (an ARF-6 GAP) and removes ARF-6 from phagosome membranes, facilitating PI(4,5)P2 removal and phagolysosome maturation to eliminate cells dying by linker cell-type death (LCD). Epistasis analysis showed this pathway is distinct from apoptotic engulfment genes. C. elegans genetics, in vivo imaging, epistasis analysis, Co-IP of RAB-35 and CNT-1 Developmental cell High 30220571
2018 In C. elegans, RAB-35 defines a third apoptotic cell clearance pathway acting in parallel to CED-1 and CED-5 pathways; RAB-35 promotes the switch of phagosomal PI(4,5)P2 to PI(3)P and recruitment of RAB-5 to phagosomal surfaces. Candidate GAP TBC-10 and GEFs FLCN-1 and RME-4 regulate RAB-35 activity in this context. C. elegans genetic screen, epistasis analysis, phosphoinositide imaging, cell corpse clearance assay, GFP localization PLoS genetics High 30138370
2019 IL-17A induces recruitment of Rab35 and its GEF DENND1C to the IL-17R/Act1 complex in airway smooth muscle cells; activated Rab35 is required for PKCα activation and fascin phosphorylation at Ser39, allowing F-actin interaction with myosin to form stress fibers and enhance smooth muscle contraction. Co-IP of Rab35 with IL-17R/Act1, Rab35 knockdown, PKCα activity assay, fascin phosphorylation assay, contraction assay Journal of immunology Medium 30683702
2020 Rab35 promotes formation of tunneling nanotubes (TNTs) and TNT-mediated vesicle transfer in neuronal cells; Rab35-GTP, ACAP2, ARF6-GDP, and EHD1 act in a cascade to promote TNT formation. MICAL-L1, required for Rab35's effect on neurite outgrowth, has no effect on TNTs, indicating distinct downstream pathways. Overexpression, dominant-negative expression, quantitative TNT assay, vesicle transfer assay Scientific reports Medium 33033331
2020 Rab35 controls myelin growth by forming a complex with myotubularin-related phosphatases MTMR13 and MTMR2, downregulating PI 3-phosphate levels and thereby suppressing mTORC1 activation. Targeted disruption of Rab35 leads to elevated PI 3-phosphates, hyperactive mTORC1 signaling, and focal hypermyelination in vivo; pharmacological inhibition of PI(3,5)P2 synthesis or mTORC1 rescues this. Co-IP of Rab35-MTMR13/MTMR2, conditional KO mice, phosphoinositide measurements, mTORC1 activity assay, pharmacological rescue Nature communications High 32503983
2020 Rab35's C-terminal polybasic cluster is required for plasma membrane targeting; exchanging the C-terminal region of Rab35 with Rab10 shifts localization, and reducing basic amino acids in this region shifts Rab35 to Golgi membranes. Rab35-Rab10 chimera construction, confocal microscopy of RAW264 cells expressing EGFP-fused chimeras Acta histochemica et cytochemica Medium 32873993
2021 BAG3 forms a complex with HSP70 and TBC1D10B (EPI64B, a Rab35 GAP); this complex attenuates TBC1D10B's ability to inactivate RAB35, supporting RAB35 activation and HRS recruitment for ESCRT-mediated endosomal tau clearance. Loss of BAG3 leads to impaired tau clearance. Mass spectrometry of BAG3 interactors, Co-IP, live-cell imaging, immunohistochemistry in AD brains, P301S tau mouse model Biological psychiatry Medium 35000752
2021 Rab35 negatively regulates Aβ production by sorting APP and BACE1 out of the endosomal network; Rab35 coordinates distinct trafficking steps for BACE1 (via effector OCRL) and APP (via effector ACAP2). Rab35 overexpression prevents amyloidogenic trafficking induced by high glucocorticoid levels. Rab35 overexpression/knockdown, APP/BACE1 trafficking assay, Aβ production measurement, effector-specific rescue Cell death & disease Medium 34876559
2023 Prior to migrasome formation, PIP5K1A generates PI(4,5)P2 at migrasome formation sites; accumulated PI(4,5)P2 recruits Rab35 via interaction with the C-terminal polybasic cluster of Rab35. Active Rab35 then promotes migrasome formation by recruiting and concentrating integrin α5, mediated by a direct Rab35-integrin α5 interaction. PIP5K1A recruitment assay, PI(4,5)P2 measurement, Rab35 recruitment/localization, Co-IP of Rab35-integrin α5, migrasome quantification Cell research High 37142675
2023 DENND2B functions as a Rab35 GEF that recruits and activates Rab35 at the intercellular cytokinetic bridge (ICB); DENND2B also interacts with active Rab35 as an effector. DENND2B knockdown delays abscission, causes F-actin accumulation at the ICB, impairs ESCRT-III recruitment, and triggers Aurora B kinase activation (NoCut checkpoint). GEF activity assay, Co-IP, siRNA knockdown, live-cell imaging, ESCRT-III localization assay, Aurora B activity assay Cell reports High 37454296
2023 The Shigella effector IcsB (a lysine Nε-fatty acylase) post-translationally acylates Rab35 in its polybasic region, non-canonically entrapping Rab35 at the bacterial-containing vacuole (BCV). Rab35 and IcsB are dispensable for initial BCV breakage but required for unwrapping damaged BCV remnants from Shigella. STED super-resolution imaging, IcsB fatty acylase activity assay, post-translational modification assay, genetic complementation, Shigella invasion assay Cell reports High 38568808
2023 DENND1A and folliculin (FLCN) are distinct Rab35 GEFs required for Podocalyxin (PODXL) trafficking under different conditions: in 3D cysts, only DENND1A KO causes polarity inversion similar to Rab35 KO; in 2D culture, FLCN knockdown causes PODXL accumulation in actin-rich structures as in Rab35 knockdown. This defines two functional cascades: FLCN-Rab35-OCRL (2D) and DENND1A-Rab35-ACAP2 (3D). CRISPR KO, siRNA knockdown, rescue with GEF-activity domain, fluorescence microscopy, MDCK cell culture The Journal of biological chemistry High 31992598
2023 USP32 (a deubiquitylating enzyme) binds Rab35 and protects it from proteasomal degradation by reducing Lys48-ubiquitination of Rab35, thereby maintaining elevated Rab35 levels in imatinib-resistant GISTs and promoting exosome secretion and drug resistance transmission. Co-IP of USP32-Rab35, ubiquitination assay (K48-specific), proteasome inhibitor rescue, siRNA knockdown, exosome secretion assay Oncogene Medium 36725886
2024 A homozygous missense variant in the GTPase fold of RAB35 (R27H) found in a patient with neurodevelopmental disorder causes enhanced interaction with DENND1A and decreased interaction with effector MICAL1, indicating an inactive conformation. This variant activates Arf6 (normally under negative Rab35 control), delays cytokinesis, and alters primary cilia length, number, and Arl13b composition. Exome sequencing, Co-IP interaction assays for DENND1A and MICAL1, Arf6 activity assay, cytokinesis assay, cilia analysis The Journal of biological chemistry High 38432637
2024 RAB35 is essential for early embryogenesis in mice (homozygous KO arrests at E7.5); conditional brain-specific KO causes severe hippocampal lamination defects due to impaired pyramidal neuron distribution. Loss of RAB35 affects levels of other RABs and cell adhesion molecules including contactin-2. Conditional KO mice, LacZ reporter, histology, immunofluorescence, quantitative proteomics Communications biology High 37085665
2024 Congenital Rab35 KO in mice causes embryonic lethality; conditional loss in kidney/ureter causes hydronephrosis associated with disrupted actin, altered Arf6 polarity, reduced adherens junctions, loss of tight junctions, defective EGFR localization, and shortened primary cilia. Conditional KO mice, histology, immunofluorescence, western blotting Journal of the American Society of Nephrology : JASN High 38530365
2024 MICAL1 is recruited by active Rab35 to HIV-1 budding sites, where it locally depolymerizes cortical actin to promote viral budding and ESCRT scission machinery recruitment; depletion of Rab35 impairs viral release and mimics MICAL1 depletion. siRNA depletion, super-resolution microscopy, live imaging, viral release assay, ESCRT recruitment assay bioRxivpreprint Medium bio_10.1101_2024.10.07.616958

Source papers

Stage 0 corpus · 95 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Rab35 regulates an endocytic recycling pathway essential for the terminal steps of cytokinesis. Current biology : CB 311 16950109
2011 Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis. Nature cell biology 241 21706022
2019 Long non-coding RNA HOTAIR promotes exosome secretion by regulating RAB35 and SNAP23 in hepatocellular carcinoma. Molecular cancer 230 30943982
2010 The Connecdenn DENN domain: a GEF for Rab35 mediating cargo-specific exit from early endosomes. Molecular cell 164 20159556
2008 Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein. The EMBO journal 151 18354496
2012 An ARF6/Rab35 GTPase cascade for endocytic recycling and successful cytokinesis. Current biology : CB 129 22226746
2008 Rab35 and its GAP EPI64C in T cells regulate receptor recycling and immunological synapse formation. The Journal of biological chemistry 126 18450757
2012 Rab35 regulates Arf6 activity through centaurin-β2 (ACAP2) during neurite outgrowth. Journal of cell science 124 22344257
2018 LRRK2 kinase regulates α-synuclein propagation via RAB35 phosphorylation. Nature communications 122 30150626
2009 Rab35 controls actin bundling by recruiting fascin as an effector protein. Science (New York, N.Y.) 119 19729655
2016 Rab35 GTPase: A Central Regulator of Phosphoinositides and F-actin in Endocytic Recycling and Beyond. Traffic (Copenhagen, Denmark) 114 27329675
2012 Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration. Journal of cell science 106 23264734
2016 Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway. The Journal of neuroscience : the official journal of the Society for Neuroscience 101 27535913
2016 Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening. Nature communications 96 27040773
2011 MICAL-L1 is a tubular endosomal membrane hub that connects Rab35 and Arf6 with Rab8a. Traffic (Copenhagen, Denmark) 91 21951725
2009 Rab35 regulates neurite outgrowth and cell shape. FEBS letters 80 19289122
2015 Rab35 GTPase Triggers Switch-like Recruitment of the Lowe Syndrome Lipid Phosphatase OCRL on Newborn Endosomes. Current biology : CB 79 26725203
2023 The phosphatidylinositol (4,5)-bisphosphate-Rab35 axis regulates migrasome formation. Cell research 76 37142675
2017 Rab35 GTPase recruits NDP52 to autophagy targets. The EMBO journal 75 28848034
2010 The connecdenn family, Rab35 guanine nucleotide exchange factors interfacing with the clathrin machinery. The Journal of biological chemistry 73 20154091
2011 Rab35 regulates phagosome formation through recruitment of ACAP2 in macrophages during FcγR-mediated phagocytosis. Journal of cell science 72 22045739
2014 Rab35 promotes the recruitment of Rab8, Rab13 and Rab36 to recycling endosomes through MICAL-L1 during neurite outgrowth. Biology open 71 25086062
2010 Rab35 mediates transport of Cdc42 and Rac1 to the plasma membrane during phagocytosis. Molecular and cellular biology 64 20065041
2011 Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell structure and function 60 21737958
2017 Planar polarized Rab35 functions as an oscillatory ratchet during cell intercalation in the Drosophila epithelium. Nature communications 54 28883443
2013 Rab35 is required for Wnt5a/Dvl2-induced Rac1 activation and cell migration in MCF-7 breast cancer cells. Cellular signalling 50 23353182
2015 Sorting of Clathrin-Independent Cargo Proteins Depends on Rab35 Delivered by Clathrin-Mediated Endocytosis. Traffic (Copenhagen, Denmark) 49 25988331
2010 Recycling of the Ca2+-activated K+ channel, KCa2.3, is dependent upon RME-1, Rab35/EPI64C, and an N-terminal domain. The Journal of biological chemistry 48 20360009
2012 Whacked and Rab35 polarize dynein-motor-complex-dependent seamless tube growth. Nature cell biology 47 22407366
2011 Connecdenn 3/DENND1C binds actin linking Rab35 activation to the actin cytoskeleton. Molecular biology of the cell 47 22072793
2010 Rab35--a vesicular traffic-regulating small GTPase with actin modulating roles. FEBS letters 46 19931531
2012 TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes. Traffic (Copenhagen, Denmark) 40 22762500
2015 Intracellular Uropathogenic E. coli Exploits Host Rab35 for Iron Acquisition and Survival within Urinary Bladder Cells. PLoS pathogens 39 26248231
2020 Rab35 and its effectors promote formation of tunneling nanotubes in neuronal cells. Scientific reports 38 33033331
2019 Rab35 controls cilium length, function and membrane composition. EMBO reports 38 31432619
2015 Activation-Inactivation Cycling of Rab35 and ARF6 Is Required for Phagocytosis of Zymosan in RAW264 Macrophages. Journal of immunology research 37 26229970
2014 Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination. Molecular biology of the cell 37 24600047
2016 Rab35 Functions in Axon Elongation Are Regulated by P53-Related Protein Kinase in a Mechanism That Involves Rab35 Protein Degradation and the Microtubule-Associated Protein 1B. The Journal of neuroscience : the official journal of the Society for Neuroscience 35 27383602
2015 MicroRNA-720 promotes in vitro cell migration by targeting Rab35 expression in cervical cancer cells. Cell & bioscience 35 26413265
2018 Rab35 GTPase and cancer: Linking membrane trafficking to tumorigenesis. Traffic (Copenhagen, Denmark) 34 29314576
2017 Rab35 protein regulates evoked exocytosis of endothelial Weibel-Palade bodies. The Journal of biological chemistry 31 28566286
2016 Increased Rab35 expression is a potential biomarker and implicated in the pathogenesis of Parkinson's disease. Oncotarget 31 27509057
2022 Transcellular propagation of fibrillar α-synuclein from enteroendocrine to neuronal cells requires cell-to-cell contact and is Rab35-dependent. Scientific reports 28 35264710
2017 Coordination of synaptic vesicle trafficking and turnover by the Rab35 signaling network. Small GTPases 28 28129039
2020 Rab35-regulated lipid turnover by myotubularins represses mTORC1 activity and controls myelin growth. Nature communications 26 32503983
2018 The small GTPase RAB-35 defines a third pathway that is required for the recognition and degradation of apoptotic cells. PLoS genetics 26 30138370
2016 EGF-stimulated activation of Rab35 regulates RUSC2-GIT2 complex formation to stabilize GIT2 during directional lung cancer cell migration. Cancer letters 26 27238570
2012 Rab35 regulates cadherin-mediated adherens junction formation and myoblast fusion. Molecular biology of the cell 26 23197472
2023 The Chlamydia effector CpoS modulates the inclusion microenvironment and restricts the interferon response by acting on Rab35. mBio 25 37530528
2016 Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation. Histochemistry and cell biology 25 26791531
2021 BAG3 Regulation of RAB35 Mediates the Endosomal Sorting Complexes Required for Transport/Endolysosome Pathway and Tau Clearance. Biological psychiatry 24 35000752
2021 Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production. Cell death & disease 23 34876559
2018 A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration. Nature communications 23 29662076
2018 Rab35-dependent extracellular nanovesicles are required for induction of tumour supporting stroma. Nanoscale 23 29693684
2023 Deubiquitylation of Rab35 by USP32 promotes the transmission of imatinib resistance by enhancing exosome secretion in gastrointestinal stromal tumours. Oncogene 21 36725886
2021 MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells. Aging 21 34500436
2018 RAB-35 and ARF-6 GTPases Mediate Engulfment and Clearance Following Linker Cell-Type Death. Developmental cell 21 30220571
2019 RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis. Molecular human reproduction 20 31152166
2017 Folliculin Interacts with Rab35 to Regulate EGF-Induced EGFR Degradation. Frontiers in pharmacology 18 29018350
2016 Enterohaemorrhagic E. coli modulates an ARF6:Rab35 signaling axis to prevent recycling endosome maturation during infection. Journal of molecular biology 18 27261256
2012 Trafficking cascades mediated by Rab35 and its membrane hub effector, MICAL-L1. Communicative & integrative biology 18 23060965
2019 Combined Assessment of Serum Alpha-Synuclein and Rab35 is a Better Biomarker for Parkinson's Disease. Journal of clinical neurology (Seoul, Korea) 16 31591837
2019 IL-17A Recruits Rab35 to IL-17R to Mediate PKCα-Dependent Stress Fiber Formation and Airway Smooth Muscle Contractility. Journal of immunology (Baltimore, Md. : 1950) 15 30683702
2015 Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-β2/ACAP2 during neurite outgrowth of PC12 cells. The Journal of biological chemistry 15 25694427
2018 EGF Stimulates Rab35 Activation and Gastric Cancer Cell Migration by Regulating DENND1A-Grb2 Complex Formation. Frontiers in pharmacology 14 30524285
2021 Rab35 regulates skeletogenesis and gastrulation by facilitating actin remodeling and vesicular trafficking. Cells & development 12 33842922
2015 Measurement of Rab35 activity with the GTP-Rab35 trapper RBD35. Methods in molecular biology (Clifton, N.J.) 12 25800845
2021 The dual functions of Rab11 and Rab35 GTPases-regulation of cell division and promotion of tumorigenicity. American journal of cancer research 9 34094658
2020 Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures. The Journal of biological chemistry 9 31992598
2023 RAB35 is required for murine hippocampal development and functions by regulating neuronal cell distribution. Communications biology 8 37085665
2023 DENND2B activates Rab35 at the intercellular bridge, regulating cytokinetic abscission and tetraploidy. Cell reports 8 37454296
2021 miR-720 Regulates Insulin Secretion by Targeting Rab35. BioMed research international 8 33880375
2021 Proteomic analysis reveals brain Rab35 as a potential biomarker of mitragynine withdrawal in rats. Brain research bulletin 8 33901587
2022 TRX2/Rab35 Interaction Impairs Exosome Secretion by Inducing Rab35 Degradation. International journal of molecular sciences 7 35743001
2019 The small GTPase RAB-35 facilitates the initiation of phagosome maturation and acts as a robustness factor for apoptotic cell clearance. Small GTPases 7 31607221
2024 Coxiella burnetii-containing vacuoles interact with host recycling endosomal proteins Rab11a and Rab35 for vacuolar expansion and bacterial growth. Frontiers in cellular and infection microbiology 4 38841112
2023 A Rab39-Klp98A-Rab35 endocytic recycling pathway is essential for rapid Golgi-dependent furrow ingression. Development (Cambridge, England) 3 37590130
2024 Rab35 Is Required for Embryonic Development and Kidney and Ureter Homeostasis through Regulation of Epithelial Cell Junctions. Journal of the American Society of Nephrology : JASN 2 38530365
2024 Post-translational targeting of Rab35 by the effector IcsB of Shigella determines intracellular bacterial niche formation. Cell reports 2 38568808
2024 Role of Rab35 in modulating lipid metabolism and viral entry during pseudorabies virus infection. International journal of biological macromolecules 2 39528177
2022 Stratum is required for both apical and basolateral transport through stable expression of Rab10 and Rab35 in Drosophila photoreceptors. Molecular biology of the cell 2 35767331
2020 Rab35 Targeting to the Plasma Membrane Is Dependent on the C-terminal Polybasic Cluster. Acta histochemica et cytochemica 2 32873993
2024 A neurodevelopmental disorder associated with a loss-of-function missense mutation in RAB35. The Journal of biological chemistry 1 38432637
2024 New aspects of a small GTPase RAB35 in brain development and function. Neural regeneration research 1 39254551
2022 Rab35 GTPase positively regulates endocytic recycling of cardiac KATP channels. Channels (Austin, Tex.) 1 35754325
2021 Rab35 regulates insulin secretion via phogrin in pancreatic β cells. Clinical and experimental pharmacology & physiology 1 34448213
2026 RAB-35 regulates distinct steps of trogocytosis in the biting and bitten cell. bioRxiv : the preprint server for biology 0 41756979
2026 Rab35 drives the malignant progression of endometrial carcinoma by regulating the nuclear translocation of β-catenin. Experimental cell research 0 41763512
2026 Levetiracetam reverses temozolomide resistance in glioblastoma by blocking drug efflux through RAB5A/CD63-RAB35 axis. Cancer letters 0 42097495
2025 A conserved Arf-GEF modulates axonal integrity through RAB-35 by altering neuron-epidermal attachment. bioRxiv : the preprint server for biology 0 40661396
2025 The unique contributions of Rab11 and Rab35 to the completion of cell division. Biological research 0 40877912
2025 Linc01271 promotes lipid synthesis and MASLD/MASH progression via miR-149-3p/RAB35 axis. Cellular and molecular life sciences : CMLS 0 40920256
2023 Rab35 is required for embryonic development and kidney and ureter homeostasis through regulation of epithelial cell junctions. bioRxiv : the preprint server for biology 0 37745459
2021 Functional variant rs17525453 within RAB35 gene promoter is possibly associated with increased risk of Parkinson's disease in Taiwanese population. Neurobiology of aging 0 34275689
2021 Rab35 controls formation of luminal projections required for bile canalicular morphogenesis. The Journal of cell biology 0 34515738

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