Affinage

USP1

Ubiquitin carboxyl-terminal hydrolase 1 · UniProt O94782

Length
785 aa
Mass
88.2 kDa
Annotated
2026-06-10
100 papers in source corpus 62 papers cited in narrative 62 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

USP1 is a nuclear cysteine-protease deubiquitinase that operates as a central regulator of the DNA damage response, removing monoubiquitin from FANCD2 (at K561) and PCNA (at K164) to control Fanconi anemia interstrand-crosslink repair, translesion synthesis, and homologous recombination (PMID:15694335, PMID:19217432, PMID:30456385). It functions as an obligate heterodimer with the activator UAF1/WDR48, which binds a conserved motif in the USP1 Fingers subdomain and activates catalysis by lowering the pKa of the catalytic histidine to enable general-base catalysis at neutral pH, with the regulatory insert L1 serving as an allosteric hub that integrates both UAF1-mediated and substrate (DNA/PCNA)-driven activation (PMID:22439892, PMID:22701671, PMID:33619839). Substrate engagement is sculpted further by accessory factors and intrinsic features: a USP1 N-terminal sequence confers FANCD2 specificity, DNA binding by UAF1 (or substitutively by RAD51AP1) is required for efficient FANCD2 deubiquitination, and ELG1 directs the complex specifically toward PCNA (PMID:20147293, PMID:31253762, PMID:30456385). Cryo-EM of USP1-UAF1 bound to monoubiquitinated FANCI-FANCD2 shows that catalysis is accompanied by UAF1-FANCI interface contacts and conformational changes in the substrate (PMID:33795880). USP1 abundance and chromatin residence are tightly gated: it is degraded in G1 by APC/C(Cdh1) to permit PCNA monoubiquitination, protected in mitosis by CDK phosphorylation, and recycled from active replication forks via autocleavage at a diglycine motif, with the metalloprotease Spartan counteracting USP1 trapping (PMID:21768287, PMID:23159736, PMID:35365626, PMID:25744535). Because persistent PCNA monoubiquitination and RAD18-dependent ssDNA gap accumulation are lethal in BRCA1/2-deficient cells, USP1 is a synthetic-lethal target, and selective inhibitors (ML323, KSQ-4279) act through a cryptic hydrophobic pocket that rearranges the active site (PMID:30576655, PMID:36228090, PMID:36170365, PMID:38885312, PMID:39190802). Beyond DNA repair, USP1-UAF1 deubiquitinates and stabilizes ID1/2/3 to maintain stem-like states and centrosome control, and stabilizes substrates spanning innate immune signaling (TBK1, cGAS), autophagy (ULK1), and transcriptional/metabolic regulators, placing it at the intersection of genome maintenance, stemness, and signaling (PMID:21925315, PMID:29138248, PMID:30335599, PMID:26822809, PMID:38054892).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1998 High

    Established that USP1 is a bona fide ubiquitin-specific protease, defining its core biochemical identity before any cellular role was known.

    Evidence recombinant USP1 cleaving Ub-beta-galactosidase in vitro

    PMID:9806842

    Open questions at the time
    • No physiological substrate identified
    • No regulatory partner or cellular pathway known at this stage
  2. 2005 High

    Identified the first physiological substrate and pathway by showing USP1 deubiquitinates monoubiquitinated FANCD2, linking the enzyme to Fanconi anemia crosslink repair.

    Evidence RNAi screen, Co-IP, chromatin fractionation in human cells

    PMID:15694335

    Open questions at the time
    • Did not define the activator requirement for catalysis
    • Mechanism of substrate recognition unresolved
  3. 2009 High

    Confirmed in an organism that USP1 acts downstream in the FA pathway and is required for HR, moving beyond cell-line correlation to genetic epistasis.

    Evidence Usp1 mouse knockout, MEF analysis, Usp1/Fancd2 double KO epistasis

    PMID:19217432

    Open questions at the time
    • Molecular basis of HR defect not separated from FANCD2 deubiquitination
    • Perinatal lethality mechanism not dissected
  4. 2011 High

    Resolved how USP1 activity is restricted to the correct cell-cycle window and how substrate targeting is diversified, and broadened its substrate range to ID proteins and CHK1.

    Evidence APC/C(Cdh1)-mediated G1 degradation assays, ELG1-directed PCNA deubiquitination, ID1/2/3 and CHK1 stability assays, DT40 HR knockouts

    PMID:20147293 PMID:21389083 PMID:21482670 PMID:21768287 PMID:21925315

    Open questions at the time
    • Structural basis of UAF1/ELG1 specificity not yet defined
    • ID-protein stabilization mechanism in stemness not generalized across tissues
  5. 2012 High

    Defined the enzymatic and regulatory mechanism of the complex—UAF1 activation chemistry, nuclear import, and autocleavage-coupled turnover—explaining how catalysis and protein recycling are controlled.

    Evidence in vitro kinetics/proton inventory, NLS mutagenesis and relocation, autocleavage and N-end-rule degradation assays

    PMID:22439892 PMID:22701671 PMID:23159736

    Open questions at the time
    • Allosteric route from UAF1 to active site not visualized structurally
    • Physiological trigger of autocleavage at forks unclear at this stage
  6. 2014 High

    Provided pharmacological proof that USP1-UAF1 deubiquitination is required for the PCNA/FANCD2 DNA damage response, establishing the complex as a druggable node.

    Evidence selective inhibitor ML323 with DUB selectivity profiling and cellular ubiquitination readouts

    PMID:24531842

    Open questions at the time
    • Inhibitor binding mode unknown
    • Therapeutic context for inhibition not yet defined
  7. 2016 High

    Connected USP1 catalytic activity to replication-fork protection and genome stability, showing DNA binding and autocleavage are functionally required and that fork-associated PCNA-Ub persistence kills BRCA1-deficient cells.

    Evidence DNA-binding and truncation mutants, DNA fiber fork-protection assays, autocleavage separation-of-function mutants, RAD51AP1 interactome, centrosome assays

    PMID:26783108 PMID:26822809 PMID:27463890 PMID:30576655

    Open questions at the time
    • Distinction between catalytic and scaffolding contributions to HR incompletely resolved
    • Mechanism coupling DNA binding to activity not yet structural
  8. 2019 High

    Reconstituted FANCD2 deubiquitination from purified components to establish that DNA binding by UAF1 (or RAD51AP1) is mechanistically required for efficient substrate processing.

    Evidence fully reconstituted in vitro deubiquitination with DNA-binding mutants plus cellular validation

    PMID:31253762

    Open questions at the time
    • Substrate conformational requirements not yet visualized
    • How DNA binding is coupled to catalysis at atomic level unresolved
  9. 2021 High

    Delivered the structural and allosteric framework—crystal/cryo-EM structures and the insert L1 regulatory hub—explaining how UAF1, substrate, and DNA converge to control USP1 activity.

    Evidence X-ray and cryo-EM of USP1-UAF1 and the FANCI-FANCD2 complex, insert-deletion biochemical activity assays

    PMID:33619839 PMID:33795880

    Open questions at the time
    • Dynamics of insert L1 allostery in cells not directly observed
    • Structural basis of PCNA versus FANCD2 selectivity not co-resolved
  10. 2022 High

    Defined the proximal mediators of USP1 synthetic lethality and visualized inhibitor mechanism, mechanistically grounding USP1 inhibitors as cancer therapeutics.

    Evidence genome-wide CRISPR screen with PCNA K164R rescue (RAD18/UBE2K), live-cell single-molecule tracking of autocleavage-dependent recycling with Spartan epistasis, cryo-EM of ML323 cryptic-site binding

    PMID:35365626 PMID:36170365 PMID:36228090

    Open questions at the time
    • Determinants of which BRCA-deficient tumors respond not fully defined
    • Resistance mechanisms incompletely mapped
  11. 2024 High

    Linked USP1 dependency to a measurable ssDNA-gap phenotype and refined the medicinal-chemistry basis of clinical-stage inhibitors.

    Evidence ssDNA gap assays with RAD18 and MRE11/EXO1 epistasis, patient-derived organoids, cryo-EM of KSQ-4279 binding with thermal-stability profiling

    PMID:38180818 PMID:38885312 PMID:39190802

    Open questions at the time
    • Biomarker translation to patient selection not established in the corpus
    • Combination strategies not mechanistically resolved here

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how USP1 substrate selectivity and ubiquitin-linkage specificity are determined across its large non-DNA-repair substrate set, and which of these many reported substrates reflect direct, physiologically dominant deubiquitination events.
  • Most non-canonical substrate studies are single-lab Co-IP/ubiquitination assays without reconstitution
  • Linkage specificity (K48/K63/K11/K27) varies by substrate and lacks a unifying structural rationale
  • Tissue- and context-dependence of substrate choice unmapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 6 GO:0003677 DNA binding 3 GO:0016787 hydrolase activity 3 GO:0098772 molecular function regulator activity 2
Localization
GO:0000228 nuclear chromosome 3 GO:0005634 nucleus 2 GO:0005829 cytosol 1
Pathway
R-HSA-73894 DNA Repair 5 R-HSA-69306 DNA Replication 4 R-HSA-1640170 Cell Cycle 3 R-HSA-168256 Immune System 3 R-HSA-392499 Metabolism of proteins 3
Partners
ELG1FANCD2FANCIID1PCNARAD51AP1TBK1UAF1
Complex memberships
USP1-UAF1 (WDR48) deubiquitinase complex

Evidence

Reading pass · 62 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2005 USP1 is a deubiquitinating enzyme that physically associates with FANCD2 and deubiquitinates monoubiquitinated FANCD2; inhibition of USP1 leads to hyperaccumulation of monoubiquitinated FANCD2, and USP1 co-localizes with FANCD2 in chromatin after DNA damage. RNAi screen, Co-IP, chromatin fractionation, chromosomal aberration assay Molecular cell High 15694335
2009 Targeted deletion of mouse Usp1 results in elevated perinatal lethality, male infertility, crosslinker hypersensitivity, and an FA phenotype; Usp1−/− MEFs show heightened monoubiquitinated Fancd2 in chromatin, impaired Fancd2 foci assembly, and a defect in homologous recombination repair. Double knockout of Usp1 and Fancd2 produces a more severe phenotype than either single knockout, placing Usp1 downstream in the FA pathway. Targeted gene knockout (mouse), MEF analysis, epistasis (double KO), chromosomal aberration assay, HR repair assay Developmental cell High 19217432
1998 USP1 encodes a 785-amino-acid ubiquitin-specific protease; recombinant USP1 protein displays genuine UBP deubiquitinase activity, correctly cleaving Ub-beta-galactosidase to produce ubiquitin and beta-galactosidase. Recombinant protein expression, in vitro enzymatic cleavage assay Genomics High 9806842
2010 Human ELG1 interacts with the USP1-UAF1 deubiquitinase complex and specifically directs it toward PCNA deubiquitination (but not FANCD2 deubiquitination); a conserved N-terminal domain of ELG1 is required for USP1-UAF1 interaction and PCNA monoubiquitination downregulation. Co-IP, RNAi knockdown, domain mapping The Journal of biological chemistry Medium 20147293
2011 USP1 acts in complex with UAF1 to promote homologous recombination (HR)-mediated double-strand break repair; USP1/UAF1-deficient chicken DT40 cells show sensitivity to camptothecin and PARP inhibitors, and disruption of NHEJ in UAF1-deficient cells restores resistance, indicating that USP1/UAF1 promotes HR at least partly by suppressing NHEJ. Gene knockout in chicken DT40 cells, drug sensitivity assay, epistasis with NHEJ disruption Molecular and cellular biology High 21482670
2011 USP1 deubiquitinates ID1, ID2, and ID3 proteins (inhibitors of DNA binding), thereby stabilizing them and promoting a stem cell-like mesenchymal state in osteosarcoma; USP1 knockdown precipitates ID protein destabilization, cell-cycle arrest, and osteogenic differentiation. Co-IP, ubiquitination assay, RNAi knockdown, protein stability assay, mouse knockout phenotype (osteopenia) Cell High 21925315
2011 USP1 is degraded in G1 phase via APC/C(Cdh1)-mediated ubiquitination; low USP1 levels in G1 are required to permit PCNA monoubiquitination in response to UV damage; expression of a degradation-resistant USP1 mutant inhibits PCNA monoubiquitination during G1, compromising translesion synthesis polymerase recruitment. Cell-cycle synchronization, protein stability assay, APC/C(Cdh1) substrate analysis, PCNA ubiquitination assay The Journal of cell biology High 21768287
2011 USP1 depletion stimulates DDB1-dependent degradation of phosphorylated CHK1; USP1 maintains total and phosphorylated CHK1 levels in response to genotoxic stress, both in a FANCD2-monoubiquitination-dependent and -independent manner, placing USP1 in a feedback circuit that downregulates CHK1 after DNA damage. RNAi knockdown, protein stability assay, epistasis with monoubiquitinated FANCD2 Human molecular genetics Medium 21389083
2012 USP1 undergoes autocleavage at a conserved G670/G671 diglycine motif, producing a C-terminal fragment (Gln-Usp1(Ct)) that is targeted for degradation by the Arg/N-end rule pathway through deamidation of its N-terminal Gln by Ntaq1; metabolic stabilization of Gln-Usp1(Ct) decreases PCNA monoubiquitination and causes hypersensitivity to UV irradiation. In vivo autocleavage assay, N-end rule pathway analysis, PCNA ubiquitination assay, UV sensitivity assay Molecular cell High 23159736
2012 UAF1 activates USP1 by modulating its active site conformation: UAF1 lowers the pKa of the catalytic histidine in USP1 by ~0.43 pH units (without changing the catalytic cysteine pKa), facilitating general base catalysis at neutral pH. USP1/UAF1 uses a general base catalysis mechanism (not an ion-pair mechanism), with a kinetic isotope effect of ~2.8–3.0 and single proton transfer in the transition state. In vitro kinetic analysis (pH-dependent inactivation, solvent kinetic isotope effect, proton inventory), active site mutagenesis Biochemistry High 22439892
2012 USP1 contains two nuclear localization signals (NLSs) that mediate nuclear import of the USP1/UAF1 complex; USP1 and UAF1 form a complex in the cytoplasm that subsequently translocates to the nucleus via USP1 NLSs. The UAF1-binding site was mapped to a conserved ~100 amino acid motif in the Fingers subdomain of USP1 (residues 420–520). Nuclear localization signal mutagenesis, cellular relocation assay, deletion mapping PloS one Medium 22701671
2013 CAPNS1 (calpain regulatory subunit) is required for USP1 stability; calpain stabilizes USP1 by activating Cdk5, which in turn inhibits Cdh1 and thereby prevents APC/C(Cdh1)-mediated USP1 degradation. Loss of CAPNS1 increases ubiquitinated PCNA, favors polymerase-η loading on chromatin, and increases mutagenesis. RNAi knockdown (CAPNS1), protein stability assay, rescue with Cdk5/p25 overexpression, PCNA ubiquitination and pol-η chromatin loading assay Molecular and cellular biology Medium 23589330
2014 The USP1-UAF1 complex is a key regulator of the DNA translesion synthesis (TLS) and Fanconi anemia (FA) deubiquitination pathways; ML323, a potent and selective inhibitor of USP1-UAF1, increases monoubiquitinated PCNA and FANCD2 in cells after UV/cisplatin damage, demonstrating that deubiquitination by this complex is required for the DNA damage response. Selective small-molecule inhibitor (ML323) with selectivity profiling against panel of DUBs, cell-based PCNA/FANCD2 ubiquitination assay Nature chemical biology High 24531842
2016 USP1 binds to and is stimulated by fork DNA; a truncated USP1 lacking its DNA-binding region is not stimulated by DNA and fails to localize to and protect replication forks. Persistence of monoubiquitinated PCNA at stalled replication forks is the mechanism of cell death in BRCA1-deficient cells lacking USP1. DNA-binding assay, truncation mutant analysis, replication fork protection assay (DNA fiber), live-cell imaging/localization, USP1 knockdown/inhibition in BRCA1-deficient cells Molecular cell High 30576655
2016 The USP1-UAF1 complex interacts with RAD51AP1 through UAF1; depletion of USP1 or UAF1 destabilizes RAD51AP1. The USP1-UAF1-RAD51AP1 interaction promotes a late step of HR repair (RAD51 foci resolution) independently of FANCD2 deubiquitination. Proteomics (UAF1 interactome), Co-IP, domain mapping, RNAi knockdown, RAD51 foci analysis, chromosomal aberration assay Cell cycle (Georgetown, Tex.) Medium 27463890
2016 PDGF signaling upregulates USP1 transcription through E2F transcription factors, which directly bind the Usp1 promoter; USP1 then stabilizes ID2, which is required for glioma cell survival in a PDGF-driven mouse model. Comparative transcriptomics, ChIP (E2F binding to Usp1 promoter), genetic ablation of Id2/Usp1, pharmacological USP1 inhibition, mouse glioma model Cancer research Medium 26951930
2017 The USP1-UAF1 complex stabilizes TBK1 by removing its K48-linked polyubiquitination, thereby preventing TBK1 degradation and enhancing TLR3/4 and RIG-I-induced IRF3 activation and IFN-β secretion during antiviral responses. Co-IP, ubiquitination assay (K48-linked), RNAi knockdown, IFN-β reporter assay, ML323 inhibitor in vitro/in vivo The Journal of experimental medicine Medium 29138248
2018 USP1 deubiquitinates and stabilizes KPNA2; USP1-mediated stabilization of KPNA2 is required for USP1's pro-metastatic function in breast cancer cells in vitro and in vivo. Co-IP, ubiquitination assay, RNAi knockdown, rescue experiments, mouse lung metastasis model Oncogene Medium 30531833
2018 USP1 deubiquitinates CHK1 to maintain its phosphorylated levels; USP1 also stabilizes Survivin/BIRC5 in glioblastoma stem cells and Survivin is shown to be downstream of USP1 in EWS cells; USP1 depletion reduces ID1 and CHK1 protein stability in glioblastoma. RNAi knockdown, protein stability assay, USP1 inhibitor (pimozide), in vivo mouse model Neuro-oncology Medium 26032834
2018 Upon platinum treatment, USP1 is phosphorylated by ATM and ATR kinases, binds to Snail, deubiquitinates and stabilizes Snail protein, thereby promoting tumor dissemination and conferring platinum resistance in ovarian cancer. USP1 knockout or inhibition increased platinum sensitivity and decreased metastatic dissemination in a Snail-dependent manner. Co-IP, ubiquitination assay, kinase inhibitor epistasis (ATM/ATR), in vivo mouse model, USP1 knockout/inhibition Science advances Medium 31086816
2019 Efficient FANCD2 deubiquitination by USP1-UAF1 requires DNA and DNA binding by UAF1; the DNA-binding activity of RAD51AP1 can substitute for that of UAF1 in FANCD2 deubiquitination in a reconstituted biochemical system. Both UAF1 and RAD51AP1 DNA-binding activities are important for FANCD2 deubiquitination in cells. Reconstituted biochemical deubiquitination assay with purified components, DNA-binding mutant analysis, cellular assay with UAF1/RAD51AP1 mutants Nature communications High 31253762
2020 USP1 removes K63-linked polyubiquitin chains on Akt to restrict PI3K-Akt-FoxO signaling in mouse muscle during prolonged starvation; a USP1 complex containing Dab2, TSC1/TSC2, and PHLPP1 is identified, with Dab2 essential for Akt recruitment to this complex for PI3K-Akt-FoxO inhibition. DUB screening, Co-IP, mass spectrometry, RNAi knockdown in mouse muscle, glucose uptake assay, ubiquitin linkage analysis EMBO reports Medium 32133736
2020 USP1 regulates AKT phosphorylation by stabilizing PHLPP1 (a phosphatase that dephosphorylates AKT); USP1 interacts with PHLPP1, and USP1 silencing decreases PHLPP1 half-life and increases AKT1 phosphorylation. Co-IP, GST pull-down, CHX stability assay, RNAi knockdown Journal of cancer research and clinical oncology Medium 22426999
2021 Crystal structures of human USP1-UAF1 with and without ubiquitin, and cryo-EM of USP1-UAF1 bound to monoubiquitinated FANCI-FANCD2, reveal plasticity in USP1, an extensive interface between UAF1 and FANCI required for substrate recognition, and USP1-UAF1-driven conformational changes in the FANCI-FANCD2 substrate during deubiquitination. Mutagenesis confirmed the UAF1-FANCI interface. X-ray crystallography, cryo-EM, site-directed mutagenesis, biochemical deubiquitination assay Nature structural & molecular biology High 33795880
2021 Insert L1 in USP1 is a central regulatory hub: it limits intrinsic USP1 activity (together with insert L3), and this inhibition is relieved by UAF1 binding; insert L1 also conveys substrate-dependent (DNA and PCNA) increases in USP1 activity independently of UAF1-mediated activation. Biochemical activity assays with USP1 insert deletion mutants, UAF1 activation assays, DNA/PCNA stimulation assays EMBO reports High 33619839
2021 USP1 deubiquitinates and stabilizes BCAT2 at the K229 site; BCAA increases USP1 protein at the translational level via the GCN2-eIF2α pathway, forming a feedforward axis that promotes pancreatic cancer development. DUB library screen, Co-IP, ubiquitination assay (site-specific), in vitro deubiquitination, in vivo orthotopic mouse model National science review Medium 35663242
2021 USP1 interacts with and deubiquitinates EZH2, stabilizing it downstream of a β-catenin/TCF4 transcriptional axis (β-catenin/TCF4 → USP1 transcription → EZH2 stabilization → H3K27me3-mediated gene repression) to drive glioma tumorigenesis. Co-IP, ubiquitination assay, ChIP, reporter assay, RNAi/knockdown, in vivo tumor model Cancer research Medium 30425057
2021 The molecular chaperone GRP75 recruits USP1 to inhibit K48-linked polyubiquitination of SIX1, forming a GRP75-USP1-SIX1 trimeric complex; the C-terminus of GRP75 (433–679 aa peptide-binding domain) is required for complex formation. Co-IP, ubiquitination assay, domain mapping (deletion mutants), in vitro and in vivo tumor models Oncogene Medium 34079090
2022 USP1 inhibition causes accumulation of mono- and polyubiquitinated PCNA and leads to reduced PCNA protein levels; ectopic expression of WT PCNA (but not ubiquitin-dead K164R PCNA) reverses USP1 inhibitor sensitivity. RAD18 and UBE2K (promoting PCNA mono- and polyubiquitination respectively) are mediators of USP1 dependency in BRCA1/2-mutant tumors. Genome-wide CRISPR-Cas9 screen, PCNA ubiquitination assay, rescue with PCNA K164R mutant, cell viability assay Molecular cancer therapeutics High 36228090
2022 Cells with an autocleavage-defective USP1 mutant (still able to deubiquitinate PCNA) experience more replication fork-stalling and premature fork termination; super-resolution microscopy and live-cell single-molecule tracking show that USP1 autocleavage is required for its proper recycling from sites of active DNA synthesis ('USP1-trapping'); the metalloprotease Spartan facilitates removal of USP1 molecules from DNA to counteract USP1-trapping cytotoxicity. Autocleavage-defective mutant knock-in, super-resolution microscopy, live-cell single-molecule tracking, DNA fiber assay, Spartan epistasis Nature communications High 35365626
2022 Cryo-EM at 2.5 Å resolution of the USP1-UAF1-ML323 inhibitor complex reveals that ML323 binds to a cryptic hydrophobic site in USP1, disrupting part of its hydrophobic core and inducing conformational changes in secondary structure that subtly rearrange the active site to achieve inhibition. Cryo-EM structure determination, with and without inhibitor Science advances High 36170365
2022 USP1 interacts with MAX and maintains MAX protein stability through deubiquitination; this promotes transcription of MYC target genes and contributes to chemotherapy resistance in B-cell lymphoma. Co-IP, ubiquitination assay, RNAi/shRNA knockdown, in vivo mouse lymphoma model Leukemia Medium 36352191
2022 USP1 identifies MAST1 as a substrate; USP1 interacts with MAST1, prevents its K48-linked polyubiquitination, and extends its half-life, thereby stabilizing MAST1 and promoting MAST1-mediated MEK1 activation and cisplatin resistance. CRISPR/Cas9 genome-wide dual screen, Co-IP, in vitro deubiquitination assay, ubiquitination linkage analysis (K48), half-life analysis, xenograft mouse model Theranostics Medium 35966591
2018 N-terminus of USP1 contains a FANCD2-specific binding sequence required for deubiquitination of K561 on FANCD2, whereas the N-terminus is not required for PCNA or FANCI deubiquitination; the N-terminus of USP1 is sufficient to engineer substrate specificity into a more promiscuous USP. Reconstituted in vitro deubiquitination of purified monoubiquitinated FANCD2/FANCI/PCNA, N-terminal deletion and chimera mutants Life science alliance High 30456385
2015 USP1 is phosphorylated by CDKs in mitosis, and this phosphorylation may prevent premature APC/C(Cdh1)-mediated degradation during normal cell cycle progression. The S313 phosphorylation site is not critical for PCNA deubiquitination or UAF1 binding in a cellular environment. The UAF1-binding site in USP1 maps to residues 420–520 (Fingers subdomain). Autocleavage of USP1 can occur in cis and is disrupted by the cancer-associated mutation L669P. Structure-function analysis with USP1 mutants (S313A, S313D, GG/AA, cancer-associated mutations), nuclear relocation assay, PCNA deubiquitination assay, Co-IP Molecular cancer Medium 25744535
2011 USP1 protein levels are phosphorylated by CDKs during mitosis, and this phosphorylation event may prevent premature degradation by APC/C(Cdh1) during normal cell cycle progression. Cell cycle synchronization, phosphorylation assay, CDK inhibitor treatment Cell cycle (Georgetown, Tex.) Medium 22101265
2016 USP1 autocleavage (at the GG/AA diglycine motif) is specifically required for USP1 function in ICL repair but not UV repair; an autocleavage-defective mutant Usp1(GG/AA) corrects monoubiquitinated Fancd2 levels and UV resistance in Usp1−/− MEFs, but only partially rescues MMC sensitivity and shows defective Fancd2 foci formation and HR. Autocleavage mutant knock-in in Usp1−/− MEFs, MMC/UV sensitivity assay, Fancd2 foci analysis, HR assay FEBS letters Medium 26783108
2019 TonEBP regulates PCNA polyubiquitination by sequentially recruiting E3 ligase SHPRH and then deubiquitinase USP1 to DNA damage sites; the Rel-homology domain of TonEBP is essential for interaction with both SHPRH and USP1, PCNA polyubiquitination, and cell survival after DNA damage. Co-IP, recruitment assay (chromatin/DNA damage foci), RNAi knockdown, domain deletion mapping iScience Medium 31376680
2020 UAF1 deubiquitinates and stabilizes METTL3 by removing K48-linked polyubiquitination after spinal cord injury, and the USP1/UAF1 complex specifically binds METTL3 to maintain its stability. Co-IP, ubiquitination assay (K48-linked), conditional knockout, in vivo SCI model The Journal of neuroscience Medium 36653190
2021 USP1 identifies ULK1 as a substrate, removing K63-linked ubiquitination from ULK1; USP1 depletion or inhibition causes ULK1 compartmentalization into a detergent-insoluble (urea-soluble) fraction enriched in SQSTM1/p62 and HDAC6, forming protein aggregates, and inhibits canonical autophagic flux while promoting an alternative lysosomal-mediated degradation route. Co-IP, ubiquitin linkage analysis (K63), cellular fractionation, immunofluorescence, RNAi knockdown, pimozide inhibition Autophagy Medium 30335599
2016 USP1 plays a role in regulating centrosome duplication; ectopic expression of catalytically active (but not C90S inactive) Usp1 induces centrosome amplification, abnormal mitotic spindles, chromosome missegregation, and aneuploidy. Loss of ID1 suppresses Usp1-induced centrosome amplification, placing ID1 downstream of USP1 in this process. Usp1 overexpression and C90S catalytic mutant, centrosome counting, mitotic spindle analysis, ID1 knockdown epistasis in Usp1−/− MEFs Cell cycle (Georgetown, Tex.) Medium 26822809
2020 USP1 interacts with TAZ/WWTR1 and increases TAZ protein stability by preventing its poly-ubiquitination; loss of USP1 reduces TAZ protein levels and decreases cell proliferation and migration of breast cancer cells. siRNA DUB library screen, Co-IP, ubiquitination assay, RNAi knockdown Cancers Medium 33114077
2023 USP1 interacts with the WW domain of TAZ and suppresses K11-linked polyubiquitination of TAZ to enhance TAZ stability and Hippo/TAZ signaling in HCC; USP1 depletion leads to decreased TAZ nuclear accumulation and downstream Hippo target gene expression. DUB siRNA screen, Co-IP, domain mapping (WW domain), ubiquitin linkage-specific assay (K11), RNAi knockdown, in vivo tumor model Cell death & disease Medium 37041150
2023 USP1 interacts with PARP1 and removes the ubiquitin chain at K197 of PARP1 to prevent its proteasomal degradation; GCN5 acetyltransferase acetylates USP1 at K130, enhancing USP1-PARP1 affinity and further increasing PARP1 stabilization. Proteomics/ubiquitylome analysis, Co-IP, GST pull-down, immunofluorescence, deletion mutant mapping, in vitro/in vivo tumor models Cell death & disease Medium 37821462
2024 USP1 removes K63-linked polyubiquitin chains from PARP1 and controls PARP1 chromatin trapping and PARylation activity, thereby regulating sensitivity to PARP inhibitors. Co-IP, ubiquitin linkage analysis (K63), chromatin trapping assay, PARylation assay, RNAi/knockout Science advances Medium 39536107
2024 USP1 inhibition promotes accumulation of ssDNA gaps at replication forks in BRCA1-deficient cells via RAD18-dependent PCNA monoubiquitination; RAD18 knockdown causes USP1 inhibitor resistance and suppresses ssDNA gap accumulation, defining RAD18-dependent PCNA ubiquitination as the proximal mediator of USP1 dependency. ssDNA gap assay, PCNA ubiquitination assay, RAD18 knockdown rescue, patient-derived organoid assay Cancer research High 38885312
2024 USP1 inhibitor KSQ-4279 (RO7623066) binds to the same cryptic hydrophobic site as ML323 in USP1 but disrupts the protein structure in subtly different ways; inhibitor binding drives a substantial increase in USP1 thermal stability, mediated through filling a hydrophobic tunnel-like pocket. Cryo-EM structure determination, biochemical assays, thermal stability assay Journal of medicinal chemistry High 39190802
2024 USP1 depletion suppresses expansion of PRIMPOL-generated ssDNA gaps by preventing MRE11 and EXO1 nuclease-mediated bidirectional gap expansion; USP1's role in promoting gap accumulation is linked to its deubiquitination of PCNA, as PCNA ubiquitination prevents gap accumulation during replication. USP1 depletion, PCNA ubiquitination assay, MRE11/EXO1 epistasis, ssDNA gap assay, DSB assay Nucleic acids research Medium 38180818
2023 USP1 interacts with Aurora B and deubiquitinates it, maintaining Aurora B stability and mediating T-ALL glucocorticoid resistance; ALKBH5 enhances USP1 expression by reducing m6A modification on USP1 mRNA. Co-IP, ubiquitination assay, in vivo mouse model, ALKBH5 knockdown/overexpression, m6A assay Molecular carcinogenesis Medium 34169564
2023 USP1 interacts with PLK1, deubiquitinates it, and increases its stability; PLK1 in turn regulates LDHA expression to promote aerobic glycolysis in T-ALL cells. Co-IP, ubiquitination assay, overexpression/knockdown epistasis, metabolic assay (lactate) Blood advances Medium 36912760
2021 USP1 deubiquitinates RPS16 at K48-linked ubiquitin chains to stabilize it; USP1-C90A catalytic mutant fails to reduce K48-linked ubiquitinated RPS16, confirming enzymatic activity requirement. USP1 depletion mimics RPS16 deficiency in inhibiting HCC growth and metastasis. Mass spectrometry, Co-IP, ubiquitination assay, catalytic mutant (C90A), rescue experiments, xenograft mouse model Journal of experimental & clinical cancer research Medium 34154657
2023 USP1 deubiquitinates and stabilizes C/EBPβ; USP1 catalytic mutant fails to affect C/EBPβ stability or ubiquitination, demonstrating enzymatic activity requirement. USP1 directly interacts with C/EBPβ and its upregulation during adipocyte differentiation promotes lipid accumulation. Co-IP, ubiquitination assay, catalytic mutant (C90A), overexpression/knockdown, USP1 inhibitor (ML323) in vivo mouse model Cell death & disease Medium 38012162
2024 USP1 removes SAR1A K27-linked oligo-ubiquitination, thereby blocking SAR1A-mediated STING-COP-II transport complex assembly and STING trafficking from ER to Golgi; USP1 inhibition sustains SAR1A ubiquitination and enhances cGAS-STING pathway activation and Type I IFN production. Chemical screen (DUB inhibitors), Co-IP, ubiquitination linkage analysis (K27), STING trafficking assay, IFN reporter assay, USP1 inhibitor (ML323) Advanced science Medium 39976106
2024 UAF1-USP1 deubiquitinase complex interacts with cGAS, selectively cleaves K48-linked polyubiquitination of cGAS, and stabilizes cGAS protein expression in macrophages; this provides positive feedback for cGAS-dependent antiviral type I IFN responses. Co-IP, ubiquitin linkage analysis (K48), Uaf1 conditional knockout, ML323 inhibitor, IFN reporter assay in vivo Journal of immunology Medium 38054892
2021 USP1 deubiquitinates and stabilizes TBLR1, and this promotes survival of circulating tumor cells in hepatocellular carcinoma by regulating Wnt signaling. Co-IP, ubiquitination assay, USP1 knockout, in vitro and in vivo CTC survival assay Frontiers in oncology Low 33102219
2023 USP1 interacts with Bcr-Abl oncoprotein in the nucleus of K562 CML cells; inhibition of USP1 activity by ML323 reduces Bcr-Abl protein levels. Co-IP, Western blot, confocal microscopy, USP1 inhibitor treatment Experimental oncology Low 32602291
2021 USP1-WDR48 (UAF1) complex deubiquitinates TAK1 in vitro, reducing its polyubiquitination and stabilizing it, thereby enhancing TGF-β-mediated EMT and migration in TNBC cells. Co-IP, in vitro ubiquitination/deubiquitination assay, RNAi knockdown Cell cycle (Georgetown, Tex.) Low 33461373
2025 USP1 can be harnessed as a deubiquitinase-targeting chimera (DUBTAC) anchor; USP1-recruiting DUBTACs stabilize CFTR and the tumor suppressor UTX in a USP1-dependent manner, demonstrating that USP1's deubiquitinating activity can be re-directed to non-natural substrates via bifunctional molecules. DUBTAC chemical biology, protein stability assay, knockdown epistasis (USP1 depletion reverses stabilization), cellular assays Journal of the American Chemical Society Medium 40252079
2022 USP1 deubiquitinates and stabilizes survivin/BIRC5 in renal cell carcinoma and Ewing sarcoma; USP1 inhibition downregulates survivin by removing ubiquitin, and ML323 also upregulates DR5 by decreasing miR-216a-5p. Co-IP, ubiquitination assay, siRNA knockdown, USP1 inhibitor (ML323/pimozide), in vivo mouse xenograft Cell death & disease Medium 36153316
2020 USP1 interacts with ERα and promotes ERα stability by inhibiting ERα K48-linked poly-ubiquitination in breast cancer cells. DUB siRNA library screen, Co-IP, ubiquitination assay (K48-linked), RNAi knockdown Journal of Cancer Low 33123289
2023 USP1 stabilizes NLRP3 and AIM2 inflammasome components through deubiquitination, promoting pyroptosis in thyroid follicular cells; USP1 also facilitates p65 nuclear translocation to modulate NLRP3 transcription. Co-IP, ubiquitination assay, USP1 knockdown/inhibition, caspase-1/GSDMD-N assay, mouse HT model, AIM2 rescue epistasis Molecular medicine Low 39134949
2025 USP1 deubiquitinates and stabilizes KIF11 at the K77 site; high interstitial fluid pressure stabilizes KIF11 in HCC through USP1-mediated deubiquitination. USP1 inhibition by ML323 reduces KIF11 protein levels and suppresses tumor progression in mice. Co-IP, proteomic analysis, ubiquitination assay (site-specific K77), in vivo orthotopic mouse model, USP1 inhibitor Journal of translational medicine Low 39810156

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 The deubiquitinating enzyme USP1 regulates the Fanconi anemia pathway. Molecular cell 488 15694335
2014 A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nature chemical biology 227 24531842
2011 USP1 deubiquitinates ID proteins to preserve a mesenchymal stem cell program in osteosarcoma. Cell 224 21925315
2009 Inactivation of murine Usp1 results in genomic instability and a Fanconi anemia phenotype. Developmental cell 211 19217432
2011 Selective and cell-active inhibitors of the USP1/ UAF1 deubiquitinase complex reverse cisplatin resistance in non-small cell lung cancer cells. Chemistry & biology 194 22118673
2018 USP1 Is Required for Replication Fork Protection in BRCA1-Deficient Tumors. Molecular cell 158 30576655
2013 Small-molecule inhibitors of USP1 target ID1 degradation in leukemic cells. Molecular cancer therapeutics 156 24130053
2013 USP1 deubiquitinase: cellular functions, regulatory mechanisms and emerging potential as target in cancer therapy. Molecular cancer 150 23937906
2010 Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. The Journal of biological chemistry 122 20147293
2019 USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. Science advances 111 31086816
2011 The USP1/UAF1 complex promotes double-strand break repair through homologous recombination. Molecular and cellular biology 107 21482670
2020 USP1 deubiquitinates Akt to inhibit PI3K-Akt-FoxO signaling in muscle during prolonged starvation. EMBO reports 106 32133736
2018 USP1 inhibition destabilizes KPNA2 and suppresses breast cancer metastasis. Oncogene 103 30531833
2010 Hematopoietic stem cell defects in mice with deficiency of Fancd2 or Usp1. Stem cells (Dayton, Ohio) 96 20506303
2015 USP1 targeting impedes GBM growth by inhibiting stem cell maintenance and radioresistance. Neuro-oncology 92 26032834
2017 Blockade of Deubiquitylating Enzyme USP1 Inhibits DNA Repair and Triggers Apoptosis in Multiple Myeloma Cells. Clinical cancer research : an official journal of the American Association for Cancer Research 82 28270494
2017 USP1-UAF1 deubiquitinase complex stabilizes TBK1 and enhances antiviral responses. The Journal of experimental medicine 69 29138248
2023 Ubiquitinated PCNA Drives USP1 Synthetic Lethality in Cancer. Molecular cancer therapeutics 62 36228090
2012 The auto-generated fragment of the Usp1 deubiquitylase is a physiological substrate of the N-end rule pathway. Molecular cell 62 23159736
2011 APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage. The Journal of cell biology 58 21768287
2014 Synthesis and structure-activity relationship studies of N-benzyl-2-phenylpyrimidin-4-amine derivatives as potent USP1/UAF1 deubiquitinase inhibitors with anticancer activity against nonsmall cell lung cancer. Journal of medicinal chemistry 57 25229643
2011 USP1 deubiquitinase maintains phosphorylated CHK1 by limiting its DDB1-dependent degradation. Human molecular genetics 56 21389083
2019 DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nature communications 55 31253762
2018 USP1 (ubiquitin specific peptidase 1) targets ULK1 and regulates its cellular compartmentalization and autophagy. Autophagy 54 30335599
2021 Diet high in branched-chain amino acid promotes PDAC development by USP1-mediated BCAT2 stabilization. National science review 53 35663242
2018 Aberrant Activation of β-Catenin Signaling Drives Glioma Tumorigenesis via USP1-Mediated Stabilization of EZH2. Cancer research 53 30425057
2021 USP1-dependent RPS16 protein stability drives growth and metastasis of human hepatocellular carcinoma cells. Journal of experimental & clinical cancer research : CR 49 34154657
2012 A noncanonical cysteine protease USP1 is activated through active site modulation by USP1-associated factor 1. Biochemistry 46 22439892
2022 USP1-trapping lesions as a source of DNA replication stress and genomic instability. Nature communications 45 35365626
2021 Structural basis of FANCD2 deubiquitination by USP1-UAF1. Nature structural & molecular biology 45 33795880
2020 USP1 Regulates TAZ Protein Stability Through Ubiquitin Modifications in Breast Cancer. Cancers 45 33114077
2024 The USP1 Inhibitor KSQ-4279 Overcomes PARP Inhibitor Resistance in Homologous Recombination-Deficient Tumors. Cancer research 41 39402989
2023 USP1-regulated reciprocal differentiation of Th17 cells and Treg cells by deubiquitinating and stabilizing TAZ. Cellular & molecular immunology 40 36600049
2022 Inhibition of USP1 activates ER stress through Ubi-protein aggregation to induce autophagy and apoptosis in HCC. Cell death & disease 39 36357365
2012 USP1 regulates AKT phosphorylation by modulating the stability of PHLPP1 in lung cancer cells. Journal of cancer research and clinical oncology 39 22426999
2022 CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells. Theranostics 38 35966591
2022 Inhibition of USP1 enhances anticancer drugs-induced cancer cell death through downregulation of survivin and miR-216a-5p-mediated upregulation of DR5. Cell death & disease 38 36153316
1998 Identification and chromosomal assignment of USP1, a novel gene encoding a human ubiquitin-specific protease. Genomics 38 9806842
2020 Targeting USP1-dependent KDM4A protein stability as a potential prostate cancer therapy. Cancer science 36 32133742
2021 A new role of GRP75-USP1-SIX1 protein complex in driving prostate cancer progression and castration resistance. Oncogene 35 34079090
2023 Deubiquitinase USP1 enhances CCAAT/enhancer-binding protein beta (C/EBPβ) stability and accelerates adipogenesis and lipid accumulation. Cell death & disease 34 38012162
2016 Gene silencing of USP1 by lentivirus effectively inhibits proliferation and invasion of human osteosarcoma cells. International journal of oncology 34 27840911
2009 Inactivation of PadR, the repressor of the phenolic acid stress response, by molecular interaction with Usp1, a universal stress protein from Lactobacillus plantarum, in Escherichia coli. Applied and environmental microbiology 34 19542339
2016 Translational regulation of the mRNA encoding the ubiquitin peptidase USP1 involved in the DNA damage response as a determinant of Cisplatin resistance. Cell cycle (Georgetown, Tex.) 33 26825230
2023 USP1 modulates hepatocellular carcinoma progression via the Hippo/TAZ axis. Cell death & disease 32 37041150
2022 Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site. Science advances 32 36170365
2020 The deubiquitinating enzyme USP1 modulates ERα and modulates breast cancer progression. Journal of Cancer 32 33123289
2018 MicroRNA-192-5p suppresses the initiation and progression of osteosarcoma by targeting USP1. Oncology letters 31 29731868
2016 PDGF Engages an E2F-USP1 Signaling Pathway to Support ID2-Mediated Survival of Proneural Glioma Cells. Cancer research 31 26951930
2016 The USP1-UAF1 complex interacts with RAD51AP1 to promote homologous recombination repair. Cell cycle (Georgetown, Tex.) 31 27463890
2024 CircUSP1 as a novel marker promotes gastric cancer progression via stabilizing HuR to upregulate USP1 and Vimentin. Oncogene 30 38366146
2023 USP1 promotes cholangiocarcinoma progression by deubiquitinating PARP1 to prevent its proteasomal degradation. Cell death & disease 30 37821462
2022 Inhibition of USP1 reverses the chemotherapy resistance through destabilization of MAX in the relapsed/refractory B-cell lymphoma. Leukemia 30 36352191
2021 ALKBH5-mediated m6A-demethylation of USP1 regulated T-cell acute lymphoblastic leukemia cell glucocorticoid resistance by Aurora B. Molecular carcinogenesis 30 34169564
2012 Two nuclear localization signals in USP1 mediate nuclear import of the USP1/UAF1 complex. PloS one 30 22701671
2022 USP1 inhibition suppresses the progression of osteosarcoma via destabilizing TAZ. International journal of biological sciences 29 35637948
2023 USP1/UAF1-Stabilized METTL3 Promotes Reactive Astrogliosis and Improves Functional Recovery after Spinal Cord Injury through m6A Modification of YAP1 mRNA. The Journal of neuroscience : the official journal of the Society for Neuroscience 28 36653190
2024 USP1-dependent nucleolytic expansion of PRIMPOL-generated nascent DNA strand discontinuities during replication stress. Nucleic acids research 26 38180818
2020 USP1 Maintains the Survival of Liver Circulating Tumor Cells by Deubiquitinating and Stabilizing TBLR1. Frontiers in oncology 25 33102219
2013 CAPNS1 regulates USP1 stability and maintenance of genome integrity. Molecular and cellular biology 25 23589330
2011 Insights into phosphorylation-dependent mechanisms regulating USP1 protein stability during the cell cycle. Cell cycle (Georgetown, Tex.) 25 22101265
2018 Specificity for deubiquitination of monoubiquitinated FANCD2 is driven by the N-terminus of USP1. Life science alliance 24 30456385
2024 Single-Stranded DNA Gap Accumulation Is a Functional Biomarker for USP1 Inhibitor Sensitivity. Cancer research 23 38885312
2021 USP1-WDR48 deubiquitinase complex enhances TGF-β induced epithelial-mesenchymal transition of TNBC cells via stabilizing TAK1. Cell cycle (Georgetown, Tex.) 22 33461373
2021 Inhibition of USP1 induces apoptosis via ID1/AKT pathway in B-cell acute lymphoblastic leukemia cells. International journal of medical sciences 21 33390793
2015 Structure-function analysis of USP1: insights into the role of Ser313 phosphorylation site and the effect of cancer-associated mutations on autocleavage. Molecular cancer 21 25744535
2024 Structural and Biochemical Insights into the Mechanism of Action of the Clinical USP1 Inhibitor, KSQ-4279. Journal of medicinal chemistry 20 39190802
2025 USP1 inhibition: A journey from target discovery to clinical translation. Pharmacology & therapeutics 19 40274197
2023 USP1 promotes the aerobic glycolysis and progression of T-cell acute lymphoblastic leukemia via PLK1/LDHA axis. Blood advances 19 36912760
2024 USP1 deubiquitinates PARP1 to regulate its trapping and PARylation activity. Science advances 18 39536107
2023 Human cytomegalovirus UL138 interaction with USP1 activates STAT1 in infection. PLoS pathogens 18 37289831
2023 The role of USP1 deubiquitinase in the pathogenesis and therapy of cancer. Acta biochimica Polonica 18 37331010
2019 Circular RNA USP1 regulates the permeability of blood-tumour barrier via miR-194-5p/FLI1 axis. Journal of cellular and molecular medicine 18 31654502
2021 Insert L1 is a central hub for allosteric regulation of USP1 activity. EMBO reports 17 33619839
2018 Loss of Deubiquitinase USP1 Blocks Pancreatic β-Cell Apoptosis by Inhibiting DNA Damage Response. iScience 17 30227958
2022 ML323, a USP1 inhibitor triggers cell cycle arrest, apoptosis and autophagy in esophageal squamous cell carcinoma cells. Apoptosis : an international journal on programmed cell death 16 35654870
2019 TonEBP Regulates PCNA Polyubiquitination in Response to DNA Damage through Interaction with SHPRH and USP1. iScience 16 31376680
2022 USP1 Inhibits NF-κB/NLRP3 Induced Pyroptosis through TRAF6 in Osteoblastic MC3T3-E1 Cells. Journal of musculoskeletal & neuronal interactions 15 36458391
2023 USP1 Expression Driven by EWS::FLI1 Transcription Factor Stabilizes Survivin and Mitigates Replication Stress in Ewing Sarcoma. Molecular cancer research : MCR 14 37478161
2021 USP1 Promotes GC Metastasis via Stabilizing ID2. Disease markers 14 34873426
2024 Design, synthesis, and biological evaluation of pyrido[2,3-d]pyrimidin-7(8H)-one derivatives as potent USP1 inhibitors. European journal of medicinal chemistry 13 38889606
2025 Characterization of TNG348: A Selective, Allosteric USP1 Inhibitor That Synergizes with PARP Inhibitors in Tumors with Homologous Recombination Deficiency. Molecular cancer therapeutics 12 39886906
2020 Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells. Experimental oncology 12 32602291
1988 Progesterone and antiprogesterone (RU 38486) modulation of estrogen-inducible glycoprotein (USP-1) synthesis and secretion in rat uterine epithelial cells. Endocrinology 12 3345728
2024 Multi-regulatory potency of USP1 on inflammasome components promotes pyroptosis in thyroid follicular cells and contributes to the progression of Hashimoto's thyroiditis. Molecular medicine (Cambridge, Mass.) 11 39134949
2015 Cheminformatics models based on machine learning approaches for design of USP1/UAF1 abrogators as anticancer agents. Systems and synthetic biology 11 25972987
2025 USP1 in regulation of DNA repair pathways. DNA repair 10 39848025
2025 Targeting USP1 Potentiates Radiation-Induced Type I IFN-Dependent Antitumor Immunity by Enhancing Oligo-Ubiquitinated SAR1A-Mediated STING Trafficking and Activation. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10 39976106
2022 ML323 suppresses the progression of ovarian cancer via regulating USP1-mediated cell cycle. Frontiers in genetics 10 35923700
2020 The DNA-binding activity of USP1-associated factor 1 is required for efficient RAD51-mediated homologous DNA pairing and homology-directed DNA repair. The Journal of biological chemistry 10 32350107
2019 Interaction of the Human Papillomavirus E1 Helicase with UAF1-USP1 Promotes Unidirectional Theta Replication of Viral Genomes. mBio 10 30890612
2016 The role of USP1 autocleavage in DNA interstrand crosslink repair. FEBS letters 10 26783108
2025 High interstitial fluid pressure enhances USP1-dependent KIF11 protein stability to promote hepatocellular carcinoma progression. Journal of translational medicine 9 39810156
2024 The UAF1-USP1 Deubiquitinase Complex Stabilizes cGAS and Facilitates Antiviral Responses. Journal of immunology (Baltimore, Md. : 1950) 9 38054892
2023 Inhibition of USP1 ameliorates hypertensive nephropathy through regulating oxidative stress and ferroptosis: A precise treatment via SJB3-019A nanodelivery. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 9 37949326
2016 A novel role for the deubiquitinase USP1 in the control of centrosome duplication. Cell cycle (Georgetown, Tex.) 9 26822809
2025 Harnessing the Deubiquitinase USP1 for Targeted Protein Stabilization. Journal of the American Chemical Society 8 40252079
2022 Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway. The Biochemical journal 8 36240066
2020 USP1 inhibitor ML323 enhances osteogenic potential of human dental pulp stem cells. Biochemical and biophysical research communications 8 32546349
2017 The Role of the Complex USP1/WDR48 in Differentiation and Proliferation Processes in Cancer Stem Cells. Current stem cell research & therapy 8 28302046

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