Affinage

RAD51AP1

RAD51-associated protein 1 · UniProt Q96B01

Length
352 aa
Mass
38.5 kDa
Annotated
2026-04-28
46 papers in source corpus 21 papers cited in narrative 21 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAD51AP1 is an accessory factor in homologous recombination that stimulates RAD51- and DMC1-mediated joint molecule formation by binding branched DNA structures, stabilizing recombinase-ssDNA filaments, and promoting synaptic complex assembly with duplex DNA templates including chromatinized substrates (PMID:17996711, PMID:17996710, PMID:21307306, PMID:34058198, PMID:41337480). Cryo-EM analysis reveals that RAD51AP1 contacts three sites spanning two adjacent RAD51 protomers, stabilizing the filament's N-terminal domain and protomer interface to promote nucleation and strand exchange, while CDK2-mediated phosphorylation at S277/S282 ensures dynamic engagement across consecutive HDR steps (PMID:41337480, PMID:41534830). RAD51AP1 forms a complex with UAF1 via a SUMO-interacting motif, enhancing RAD51-mediated DNA pairing and supporting DNA-dependent FANCD2 deubiquitination in the Fanconi anemia interstrand crosslink repair pathway (PMID:27239033, PMID:31253762). At ALT telomeres, MMS21-dependent SUMOylation stabilizes RAD51AP1, which binds TERRA RNA to promote R-loop and D-loop formation, generating G-quadruplex intermediates that drive RAD52-independent break-induced replication for telomere maintenance (PMID:31400850, PMID:36265486, PMID:36265488).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2006 Medium

    Establishing RAD51AP1 as a RAD51-interacting protein defined the gene's entry into the HR field: a conserved C-terminal motif was mapped as the minimal RAD51-binding region, and cellular depletion sensitized cells to crosslink damage.

    Evidence Yeast two-hybrid and co-IP in HEK293 cells with truncation/point mutation mapping; siRNA knockdown with MMC survival and metaphase spread analysis

    PMID:16920159 PMID:16990250

    Open questions at the time
    • No biochemical activity demonstrated at this stage
    • HR reporter results were ambiguous (no reduction despite increased breaks)
    • RAD51AP1's role relative to known RAD51 mediators was unclear
  2. 2007 High

    Two independent biochemical reconstitutions simultaneously demonstrated that RAD51AP1 directly stimulates RAD51-mediated D-loop formation by binding branched DNA intermediates and acting after RAD51-ssDNA filament assembly, establishing its core enzymatic function.

    Evidence In vitro D-loop assays with purified proteins, structure-specific DNA-binding assays, epistasis with XRCC3 via RNAi, RAD51 focus analysis

    PMID:17996710 PMID:17996711

    Open questions at the time
    • Structural basis of RAD51AP1–RAD51 interaction unknown
    • Mechanism of branched-DNA recognition unresolved
    • In vivo requirement for D-loop stimulation not formally tested
  3. 2011 High

    Discovery that RAD51AP1 also stimulates DMC1-mediated D-loop formation through a distinct binding interface (WVPP motif) extended the protein's role from mitotic to meiotic recombination, establishing it as a dual-recombinase accessory factor.

    Evidence Purified DMC1–RAD51AP1 reconstitution, WVPP motif mutagenesis, immunofluorescence co-localization in mouse spermatocytes

    PMID:21307306 PMID:21903585

    Open questions at the time
    • Meiotic phenotype in RAD51AP1-deficient animals not tested
    • Whether RAD51 and DMC1 binding is simultaneous or mutually exclusive in vivo was unclear
  4. 2012 High

    Mapping two distinct DNA-binding domains in RAD51AP1, both required for full activity, resolved why simple RAD51 interaction was insufficient and showed that cellular complementation requires both DNA-binding functions.

    Evidence Domain truncation/point mutation with EMSA, in vitro D-loop assay, and cellular rescue

    PMID:22375013

    Open questions at the time
    • Structural basis of each DNA-binding domain not determined
    • How the two domains coordinate during strand exchange was unknown
  5. 2014 High

    Genetic knockout revealed a replication fork maintenance function for RAD51AP1: loss slows fork elongation and delays RAD51 focus resolution, separating its replication-support role from initial RAD51 filament assembly.

    Evidence DT40 and human cell knockouts, DNA fiber assay, RAD51 focus kinetics, clonogenic survival

    PMID:25288561

    Open questions at the time
    • Whether fork slowing is a direct effect or secondary to unresolved HR intermediates was unclear
    • Relationship to fork reversal mechanisms not explored
  6. 2016 High

    Identification of a UAF1–RAD51AP1 complex formed via SUMO-like/SIM interactions revealed that UAF1 enhances RAD51-mediated synaptic complex assembly through RAD51AP1, linking deubiquitinase scaffold proteins to HR.

    Evidence Reconstituted UAF1–RAD51AP1–RAD51 complex, in vitro D-loop/synaptic assays, SIM mutagenesis, cellular complementation and RAD51 focus kinetics

    PMID:27239033 PMID:27463890

    Open questions at the time
    • Whether UAF1's HR-stimulatory role is catalytically linked to USP1 deubiquitinase activity was unresolved
    • In vivo stoichiometry of trimeric complex unknown
  7. 2019 High

    Two key expansions of RAD51AP1 function emerged: (1) its DNA-binding activity supports FANCD2 deubiquitination in the Fanconi anemia pathway via the USP1–UAF1–RAD51AP1 complex, and (2) it is essential for ALT telomere maintenance through SUMOylation-regulated HR and break-induced replication.

    Evidence Reconstituted FANCD2 deubiquitination assay with DNA-binding mutants and cellular validation; RAD51AP1 KO in ALT+ cells with telomere length, cGAS-STING, and SUMOylation assays

    PMID:31253762 PMID:31400850

    Open questions at the time
    • How RAD51AP1 coordinates FANCD2 deubiquitination timing with HR in vivo was unknown
    • Identity of the MMS21-SUMOylated lysine's downstream effectors beyond stabilization was unclear
  8. 2021 High

    Demonstration that RAD51AP1 binds nucleosome core particles and promotes joint molecule formation with chromatinized templates showed how it assists homology search in a physiological chromatin context.

    Evidence In vitro NCP-binding EMSA, histone octamer pulldown, D-loop assay with chromatinized template, C-terminal deletion mutants

    PMID:34058198

    Open questions at the time
    • Whether NCP binding occurs through histone contacts or wrapped DNA was not resolved
    • In vivo contribution of NCP binding vs. naked-DNA binding not separated
  9. 2022 High

    RAD51AP1 was shown to bind TERRA RNA and drive telomeric R-loop formation that generates G-quadruplexes enabling RAD52-independent D-loop formation—a non-canonical RNA-mediated mechanism for ALT telomere maintenance—while operating in a parallel sub-pathway to RAD54L downstream of RAD51.

    Evidence In vitro R-loop assays with TERRA, DRIP at telomeres, G4 stabilization experiments, RAD51AP1/RAD52 double KO, RAD51AP1/RAD54L double deletion epistasis with PARP inhibitors

    PMID:35652094 PMID:36265486 PMID:36265488

    Open questions at the time
    • Structural basis of TERRA recognition by RAD51AP1 unknown
    • Whether G4-mediated D-loop pathway operates at non-telomeric loci was untested
    • Relative contribution of RAD51AP1 vs. RAD54L sub-pathways in different genomic contexts unknown
  10. 2024 Medium

    Discovery that pre-rRNA binds RAD51AP1 and facilitates its recruitment to DSBs via liquid-liquid phase separation expanded the RNA-interaction repertoire beyond TERRA and linked RNA Pol I transcription to HR efficiency.

    Evidence RNA immunoprecipitation, DSB co-localization, RNA Pol I inhibitor treatment, in vitro LLPS assay with purified proteins, HR reporter

    PMID:38403248

    Open questions at the time
    • Phase separation functional importance not confirmed by mutagenesis
    • Whether pre-rRNA interaction is direct or mediated by nucleolar factors needs clarification
    • Physiological relevance of LLPS at DSBs versus nucleolar function not distinguished
  11. 2025 High

    Cryo-EM structures and CDK2 phosphorylation studies resolved the molecular mechanism: RAD51AP1 spans two RAD51 protomers via three binding sites to stabilize filaments, and CDK2-mediated phosphorylation at S277/S282 is required for dynamic turnover across HR steps, explaining why constitutive DNA binding is deleterious.

    Evidence Cryo-EM of RAD51-ssDNA filaments ± RAD51AP1, strand exchange assays, S277/282A and S277/282D mutagenesis with in vitro and cellular rescue, CDK2 kinase assay

    PMID:41337480 PMID:41534830

    Open questions at the time
    • Full-length RAD51AP1 structure not determined
    • How phosphorylation modulates NCP and RNA binding is unknown
    • Structural basis of the DMC1-binding WVPP interface remains unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: the structural basis of RAD51AP1's dual RNA-binding (TERRA, pre-rRNA) specificity, the in vivo meiotic phenotype of RAD51AP1 deficiency, how CDK2 phosphorylation coordinates with SUMOylation to regulate RAD51AP1 at telomeres versus genomic DSBs, and whether the phase-separation activity is functionally required for HR foci.
  • No animal knockout model phenotype reported
  • Phase separation mechanism lacks mutagenic validation
  • Interplay between CDK2 phosphorylation and MMS21-SUMOylation untested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 4 GO:0098772 molecular function regulator activity 4 GO:0003723 RNA binding 3 GO:0008092 cytoskeletal protein binding 1
Localization
GO:0005634 nucleus 3 GO:0005694 chromosome 3
Pathway
R-HSA-73894 DNA Repair 8 R-HSA-1852241 Organelle biogenesis and maintenance 3 R-HSA-1643685 Disease 2 R-HSA-69306 DNA Replication 2 R-HSA-1640170 Cell Cycle 1
Partners
DMC1MMS21NUCKS1RAD51RAD54LUAF1USP1
Complex memberships
USP1-UAF1-RAD51AP1

Evidence

Reading pass · 21 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2007 RAD51AP1 binds both dsDNA and D-loop structures, and stimulates the RAD51-mediated D-loop reaction only when able to physically interact with RAD51; epistasis experiments placed RAD51AP1 in the same pathway as XRCC3, and biochemical/cytological data showed it acts subsequent to RAD51-ssDNA nucleoprotein filament assembly. In vitro D-loop assay with purified proteins, DNA-binding assays, RNAi knockdown + epistasis with XRCC3, cytological RAD51 focus analysis Molecular cell High 17996711
2007 RAD51AP1 selectively binds branched DNA structures (obligatory intermediates in joint molecule formation) and physically contacts RAD51 to stimulate joint molecule/D-loop formation; both activities together confer its ability to promote homologous recombination. Purified protein DNA-binding assays with branched vs. linear substrates, in vitro joint molecule formation assay, Co-IP/pulldown with RAD51 Molecular cell High 17996710
2011 RAD51AP1 physically associates with the meiosis-specific recombinase DMC1 and stimulates DMC1-mediated D-loop reaction by enhancing the ability of the DMC1 presynaptic filament to capture duplex DNA and assemble the synaptic complex; distinct epitopes in RAD51AP1 mediate RAD51 vs. DMC1 interactions; RAD51AP1 colocalizes with DMC1 foci in mouse spermatocytes. Pulldown/Co-IP of RAD51AP1-DMC1, in vitro D-loop assay with purified DMC1, immunofluorescence co-localization in mouse spermatocytes, truncation/point mutation mapping Proceedings of the National Academy of Sciences of the United States of America High 21307306
2011 A conserved WVPP motif in RAD51AP1 is critical for DMC1 interaction but dispensable for RAD51 association, defining two distinct binding interfaces on RAD51AP1 for its two recombinase partners. Truncation and point mutation analysis of RAD51AP1, pulldown assays The Journal of biological chemistry High 21903585
2012 RAD51AP1 harbors two distinct DNA-binding domains, both required for maximal stimulation of RAD51 recombinase activity under physiological conditions; mutants impaired in either domain are non-functional in cells. Domain mapping via truncations and point mutations, in vitro DNA-binding assays (EMSA), in vitro D-loop assays, cellular complementation assays The Journal of biological chemistry High 22375013
2006 RAD51AP1 (PIR51/Pir51) interacts with RAD51 through a conserved C-terminal motif; the same structural motif is shared with RAD51AP2, and truncations/point mutations in both proteins defined the minimal RAD51-binding region (~40 amino acids). Yeast two-hybrid, co-immunoprecipitation in HEK293 cells, truncation/point mutation analysis Nucleic acids research Medium 16990250
2014 RAD51AP1 is required to maintain wild-type replication fork progression speed; RAD51AP1-deficient vertebrate cells (human and chicken DT40 knockouts) show slowed replication fork elongation, increased new-origin firing, and delayed resolution (but not impaired formation) of DNA damage-induced RAD51 foci. Targeted gene knockout in DT40 cells, DNA fiber assay (replication fork speed), RAD51 focus kinetics by immunofluorescence, clonogenic survival assays DNA repair High 25288561
2015 NUCKS1, a paralog of RAD51AP1, shares extensive sequence homology and the same DNA-binding preference as RAD51AP1 but binds DNA with reduced affinity; NUCKS1 knockdown phenocopies RAD51AP1 knockdown (impaired HR, increased MMC sensitivity, chromatid breaks, slowed replication forks). Sequence homology analysis, in vitro DNA-binding assays, siRNA knockdown, HR reporter assay, DNA fiber assay, cytogenetics Nucleic acids research Medium 26323318
2016 RAD51AP1 forms a dimeric complex with UAF1 (mediated by SUMO-like domains in UAF1 and a SUMO-interacting motif in RAD51AP1) and a trimeric complex with RAD51 through RAD51AP1; UAF1 enhances RAD51-mediated homologous DNA pairing in a RAD51AP1-dependent but USP1-independent manner; the RAD51AP1-UAF1 complex cooperates with RAD51 to assemble the synaptic complex. Purified protein complex reconstitution, in vitro D-loop/synaptic complex assays, mutagenesis of SUMO-interacting motif, cellular complementation with interaction-deficient mutants Cell reports High 27239033
2016 UAF1 mediates interaction between USP1 and RAD51AP1; USP1 or UAF1 depletion decreases RAD51AP1 stability; UAF1-interaction-deficient RAD51AP1 mutants show persistent RAD51 foci after DNA damage and increased chromosomal aberrations, indicating USP1-UAF1 regulates a late step in HR via RAD51AP1. Proteomic pulldown/mass spectrometry of UAF1 interactors, Co-IP, protein stability assays, RAD51 focus kinetics, chromosomal aberration analysis with interaction-deficient point mutants Cell cycle (Georgetown, Tex.) Medium 27463890
2019 Efficient FANCD2 deubiquitination by the USP1-UAF1 complex is DNA-dependent and requires DNA binding by UAF1; RAD51AP1's DNA-binding activity can substitute for UAF1's DNA-binding activity to support FANCD2 deubiquitination in a reconstituted biochemical system and in cells, revealing that the USP1-UAF1-RAD51AP1 complex participates in the Fanconi anemia pathway through DNA-dependent FANCD2 deubiquitination. Reconstituted biochemical deubiquitination assays with purified USP1-UAF1-RAD51AP1, DNA-binding mutants, cellular FANCD2 ubiquitination assays Nature communications High 31253762
2019 RAD51AP1 is essential for ALT-mediated telomere elongation; its disruption causes generational telomere shortening in ALT+ cells through loss of both RAD51-dependent HR and RAD52-POLD3-dependent break-induced DNA synthesis; RAD51AP1 protein levels in ALT+ cells are elevated by MMS21-associated SUMOylation, and mutation of a single SUMO-targeted lysine perturbs telomere dynamics. RAD51AP1 knockout in ALT+ cells, telomere length assays, cGAS-STING activation readout, SUMOylation assays with MMS21, site-directed mutagenesis of SUMO-targeted lysine Molecular cell High 31400850
2021 RAD51AP1 binds nucleosome core particles (NCPs) through its C-terminal DNA-binding domain; it can promote duplex DNA capture and joint molecule formation with NCP and chromatinized template DNA in vitro, suggesting it assists RAD51-mediated homology search in chromatin by anchoring the nucleosomal DNA template to the RAD51-ssDNA filament. In vitro NCP-binding assay (EMSA), pulldown of RAD51AP1 with histone octamer, in vitro D-loop assay with chromatinized template, C-terminal deletion mutants The Journal of biological chemistry High 34058198
2022 RAD51AP1 interacts with TERRA RNA and promotes telomeric R-loop formation; RAD51AP1 depletion reduces R-loop formation at telomere DNA breaks; RAD51AP1-mediated TERRA R-loops also generate G-quadruplexes (G4s) that persist after R-loop resolution, enabling D-loop formation without RAD52 and thus driving a RAD52-independent ALT pathway. In vitro R-loop formation assay with TERRA and RAD51AP1, telomere-specific R-loop immunoprecipitation (DRIP), G4 stabilization experiments, RAD51AP1 and RAD52 KO cells, BIR activity assays Molecular cell High 36265486
2022 RAD51AP1 regulates TERRA R-loop homeostasis at ALT telomeres; its interaction with TERRA and telomeric chromatin suppresses transcription-replication collisions during ALT-HDR; this non-canonical function requires RAD51AP1's intrinsic SUMO-SIM regulatory axis. Proteomic analysis, TERRA RNA immunoprecipitation, R-loop immunoprecipitation at telomeres, RAD51AP1 depletion with SUMO-SIM mutants, replication-transcription collision assays Molecular cell High 36265488
2022 RAD51AP1 and RAD54L operate in two distinct parallel sub-pathways downstream of RAD51 in homologous recombination; simultaneous deletion of both genes further sensitizes cancer cells to PARP inhibitors, mitomycin C, and hydroxyurea beyond single deletions, establishing a synthetic interaction. Double-gene deletion in human cancer cell lines, clonogenic survival assays with olaparib/MMC/HU, epistasis analysis Frontiers in cell and developmental biology Medium 35652094
2024 RAD51AP1 associates with pre-rRNA; both N- and C-termini of RAD51AP1 bind pre-rRNA; pre-rRNA co-localizes with RAD51AP1 at DSBs and facilitates RAD51AP1 recruitment to DSBs; inhibition of RNA Pol I (pre-rRNA synthesis) suppresses RAD51AP1 DSB recruitment and HR; RAD51AP1 forms liquid-liquid phase separation in the presence of pre-rRNA in vitro, potentially underlying foci formation. RNA immunoprecipitation, co-localization at DSBs, RNA Pol I inhibitor treatment, LLPS assay in vitro with purified proteins, HR reporter assay The Journal of biological chemistry Medium 38403248
2025 RAD51AP1 activity in homology-directed repair is regulated by CDK2-mediated phosphorylation at S277/282; phospho-ablating S277/282A mutations increase DNA/nucleosome binding and D-loop stimulation in vitro but fail to rescue RAD51AP1 deficiency in cells, whereas phosphomimetic S277/282D fully rescues; this indicates phosphorylation ensures dynamic RAD51AP1 engagement across consecutive HDR steps. Site-directed mutagenesis (S277/282A and S277/282D), in vitro D-loop assay, EMSA with ssDNA/dsDNA/NCP, cellular toxicity and DNA replication assays with mutant rescue, CDK2 kinase assay The Journal of biological chemistry High 41534830
2025 Cryo-EM/structural analysis reveals RAD51AP1 possesses at least three RAD51-binding sites that span two adjacent RAD51 protomers; RAD51AP1 stabilizes the RAD51 N-terminal domain and protomer interface within filaments, promotes RAD51-ssDNA filament nucleation, stabilization, and strand exchange; ATP hydrolysis to ADP causes RAD51 filament expansion and reduces RAD51-DNA binding. Cryo-EM structural analysis of RAD51-ssDNA filaments with/without RAD51AP1, biochemical strand exchange assays, mutagenesis of binding sites, structural analysis of Mg2+-ATP vs. Mg2+-ADP states Proceedings of the National Academy of Sciences of the United States of America High 41337480
2006 RAD51AP1 (Pir51) is a nuclear protein whose expression is cell-cycle regulated similarly to RAD51; siRNA depletion sensitizes cells to mitomycin C and increases double-strand breaks in metaphase spreads without reducing HR repair efficiency, placing RAD51AP1 in a RAD51-associated pathway that specifically responds to DNA crosslink damage. siRNA knockdown, clonogenic survival assays, metaphase chromosome spread analysis, HR reporter assay (I-SceI) Mutation research Medium 16920159
2025 TERRA R-loop formation at telomeres requires non-redundant functions of both RAD51 and RAD51AP1; TERRA R-loops interfere with semiconservative DNA replication, promoting break-induced replication (BIR) for telomere maintenance. TERRA overexpression system, DRIP-seq, RAD51AP1 depletion, BIR activity assay, telomere maintenance assays bioRxivpreprint Medium bio_10.1101_2025.01.09.632133

Source papers

Stage 0 corpus · 46 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 Promotion of homologous recombination and genomic stability by RAD51AP1 via RAD51 recombinase enhancement. Molecular cell 120 17996711
2007 RAD51AP1 is a structure-specific DNA binding protein that stimulates joint molecule formation during RAD51-mediated homologous recombination. Molecular cell 100 17996710
2022 TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch. Molecular cell 89 36265486
2004 Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma. World journal of gastroenterology 59 14966907
2019 RAD51AP1 Is an Essential Mediator of Alternative Lengthening of Telomeres. Molecular cell 56 31400850
2019 DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nature communications 54 31253762
2015 NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic acids research 54 26323318
2022 RAD51AP1 regulates ALT-HDR through chromatin-directed homeostasis of TERRA. Molecular cell 52 36265488
2016 Promotion of RAD51-Mediated Homologous DNA Pairing by the RAD51AP1-UAF1 Complex. Cell reports 49 27239033
2017 Role of RAD51AP1 in homologous recombination DNA repair and carcinogenesis. DNA repair 44 28963981
2012 Mechanistic insights into RAD51-associated protein 1 (RAD51AP1) action in homologous DNA repair. The Journal of biological chemistry 39 22375013
2011 Molecular basis for enhancement of the meiotic DMC1 recombinase by RAD51 associated protein 1 (RAD51AP1). Proceedings of the National Academy of Sciences of the United States of America 37 21307306
2006 Pir51, a Rad51-interacting protein with high expression in aggressive lymphoma, controls mitomycin C sensitivity and prevents chromosomal breaks. Mutation research 35 16920159
2016 The USP1-UAF1 complex interacts with RAD51AP1 to promote homologous recombination repair. Cell cycle (Georgetown, Tex.) 31 27463890
2020 RAD51AP1 Deficiency Reduces Tumor Growth by Targeting Stem Cell Self-Renewal. Cancer research 29 32665355
2020 RAD51AP1 promotes progression of ovarian cancer via TGF-β/Smad signalling pathway. Journal of cellular and molecular medicine 27 33314567
2014 RAD51AP1-deficiency in vertebrate cells impairs DNA replication. DNA repair 26 25288561
2006 RAD51AP2, a novel vertebrate- and meiotic-specific protein, shares a conserved RAD51-interacting C-terminal domain with RAD51AP1/PIR51. Nucleic acids research 26 16990250
2022 RAD51AP1 and RAD54L Can Underpin Two Distinct RAD51-Dependent Routes of DNA Damage Repair via Homologous Recombination. Frontiers in cell and developmental biology 25 35652094
2011 RAD51-associated protein 1 (RAD51AP1) interacts with the meiotic recombinase DMC1 through a conserved motif. The Journal of biological chemistry 23 21903585
2021 RAD51AP1 Loss Attenuates Colorectal Cancer Stem Cell Renewal and Sensitizes to Chemotherapy. Molecular cancer research : MCR 20 34099522
2019 Silencing of RAD51AP1 suppresses epithelial-mesenchymal transition and metastasis in non-small cell lung cancer. Thoracic cancer 19 31317661
2019 Single-cell RNA-seq reveals RAD51AP1 as a potent mediator of EGFRvIII in human glioblastomas. Aging 18 31532757
2023 Identification of the E2F1-RAD51AP1 axis as a key factor in MGMT-methylated GBM TMZ resistance. Cancer biology & medicine 16 37283490
2020 A Novel Neoplastic Fusion Transcript, RAD51AP1-DYRK4, Confers Sensitivity to the MEK Inhibitor Trametinib in Aggressive Breast Cancers. Clinical cancer research : an official journal of the American Association for Cancer Research 15 33172895
2021 RAD51AP1 mediates RAD51 activity through nucleosome interaction. The Journal of biological chemistry 12 34058198
2021 ZEB1 induces non-small cell lung cancer development by targeting microRNA-320a to increase the expression of RAD51AP1. Experimental cell research 10 34097859
2008 Germline mutations in RAD51, RAD51AP1, RAD51B, RAD51C,RAD51D, RAD52 and RAD54L do not contribute to familial chronic lymphocytic leukemia. Leukemia & lymphoma 9 18203022
2024 Identification of RAD51AP1 as a key gene in hepatitis B virus-associated hepatocellular carcinoma. Heliyon 7 39850418
2022 Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro. Journal of personalized medicine 6 35207688
2024 Pre-rRNA facilitates the recruitment of RAD51AP1 to DNA double-strand breaks. The Journal of biological chemistry 5 38403248
2024 RRM1 promotes homologous recombination and radio/chemo-sensitivity via enhancing USP11 and E2F1-mediated RAD51AP1 transcription. Cell death discovery 5 39695160
2023 miR-383-5p Regulates Preadipocyte Proliferation and Differentiation by Targeting RAD51AP1. International journal of molecular sciences 5 37762324
2022 Knockdown of RAD51AP1 suppressed cell proliferation and invasion in esophageal squamous cell carcinoma. Discover oncology 5 36197550
2025 Epigenetically elevated RAD51AP1 regulates the RAD51-UAF1 complex contributing to temozolomide resistance in EGFRvIII glioblastoma. Chinese medical journal 2 40211735
2023 E2F7/RAD51AP1 Axis Inhibits Endometrial Cancer Sensitivity to 5-FU via the Fatty Acid Metabolic Pathway. Anticancer research 2 37909953
2023 The gene RAD51AP1 promotes the progression of pancreatic cancer via the PI3K/Akt/NF-κB signaling pathway. Neoplasma 2 37962862
2023 [Research Advances of RAD51AP1 in Tumor Progression and Drug Resistance]. Zhongguo fei ai za zhi = Chinese journal of lung cancer 2 37985156
2022 TERRA and RAD51AP1 at the R&D-loop department of ALT telomeres. Molecular cell 2 36332602
2024 Knocking down RAD51AP1 enhances chemosensitivity by inhibiting the self-renewal of CD133 positive ovarian cancer stem-like cells. Discover oncology 1 39235706
2026 Phosphoregulation of RAD51AP1 function in homology-directed repair. The Journal of biological chemistry 0 41534830
2026 DLGAP5 protects glioblastoma cells against DNA damage through E2F1-transcripted RAD51AP1. Biochimica et biophysica acta. Molecular basis of disease 0 42002087
2025 Transcriptomic analysis of laser-capture microdissected tumors reveals RAD51AP1 as a tumor-specific marker associated progression from pancreatic intraepithelial neoplasia to invasive pancreatic cancer. Cancer cell international 0 40495122
2025 Phosphoregulation of RAD51AP1 function in homology-directed repair. bioRxiv : the preprint server for biology 0 40964358
2025 Research Progress of RAD51AP1 in Malignant Tumors of the Female Reproductive System. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 0 41109978
2025 RAD51AP1 is a versatile RAD51 modulator. Proceedings of the National Academy of Sciences of the United States of America 0 41337480