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RAD51AP1

RAD51-associated protein 1 · UniProt Q96B01

Length
352 aa
Mass
38.5 kDa
Annotated
2026-06-10
46 papers in source corpus 20 papers cited in narrative 20 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAD51AP1 is a structure-specific DNA-binding accessory factor that promotes RAD51-mediated homologous recombination (HR) at a step downstream of presynaptic filament assembly (PMID:17996711, PMID:17996710, PMID:25288561). It binds branched and D-loop DNA intermediates through two distinct DNA-binding domains, both required for full activity, and stimulates joint-molecule and D-loop formation only when it can simultaneously make direct physical contact with RAD51 (PMID:17996710, PMID:22375013). Cryo-EM defines the structural basis: RAD51AP1 engages at least three RAD51-binding sites spanning two adjacent RAD51 protomers, stabilizes the RAD51 N-terminal domain and protomer interface, promotes filament nucleation and stabilization, and stimulates strand exchange (PMID:41337480). It extends these functions to chromatinized templates by binding nucleosome core particles to anchor donor DNA to the filament (PMID:34058198), and CDK2-mediated phosphorylation at S277/S282 modulates its DNA- and nucleosome-binding avidity to ensure dynamic engagement across consecutive HDR steps (PMID:41534830). Beyond mitotic HR, RAD51AP1 cooperates with the meiotic recombinase DMC1 through a conserved WVPP motif distinct from its RAD51-binding epitope (PMID:21307306, PMID:21903585), and forms a SUMO-SIM-dependent complex with UAF1 that further enhances RAD51-mediated pairing and contributes DNA-binding activity to FANCD2 deubiquitination by USP1-UAF1 (PMID:27239033, PMID:31253762). At ALT telomeres, RAD51AP1 binds TERRA RNA to assemble R-loops that generate persistent G-quadruplexes, driving an R-to-D-loop switch that enables RAD52-independent break-induced replication and telomere maintenance, a function sustained by MMS21-dependent SUMOylation (PMID:31400850, PMID:36265488, PMID:36265486). RAD51AP1 also associates with pre-rRNA, which facilitates its recruitment to double-strand breaks and supports phase separation into foci (PMID:38403248).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2007 High

    Established RAD51AP1 as a post-synaptic HR factor: the question was where in the recombination pathway it acts, and reconstitution showed it stimulates D-loop/joint-molecule formation after filament assembly via combined structure-specific DNA binding and RAD51 contact.

    Evidence In vitro D-loop and joint-molecule assays with purified protein, EMSA on branched vs linear substrates, RNAi HR assays, XRCC3 epistasis

    PMID:17996710 PMID:17996711

    Open questions at the time
    • Did not resolve the structural basis of RAD51 contact
    • Number and location of RAD51-binding sites unknown
  2. 2006 Medium

    Mapped the RAD51-interaction module to a conserved C-terminal motif shared with RAD51AP2, defining the molecular determinant of recombinase binding.

    Evidence Yeast two-hybrid, truncation and point mutagenesis in HEK293, Co-IP

    PMID:16990250

    Open questions at the time
    • Functional consequence of motif mutations in HR not assessed here
    • Single-lab interaction mapping
  3. 2011 High

    Extended RAD51AP1 function to meiotic recombination by showing it stimulates DMC1 and uses a distinct WVPP epitope, separating its mitotic and meiotic recombinase partnerships.

    Evidence In vitro DMC1 D-loop/synaptic complex assays, Co-IP, WVPP mutagenesis, spermatocyte immunofluorescence

    PMID:21307306 PMID:21903585

    Open questions at the time
    • In vivo meiotic requirement not tested via knockout
    • Structural basis of WVPP-DMC1 contact unknown
  4. 2012 High

    Defined RAD51AP1 as a bipartite DNA-binding protein, showing two distinct DNA-binding domains are both required for cellular activity.

    Evidence Domain mapping by mutagenesis, EMSA, cellular complementation

    PMID:22375013

    Open questions at the time
    • Distinct biochemical roles of the two domains not separated
    • No structural model of DNA engagement
  5. 2014 High

    Connected RAD51AP1 to replication-fork integrity in vertebrate cells and confirmed it acts after RAD51 foci formation by showing impaired foci resolution rather than assembly.

    Evidence DT40 gene inactivation, DNA fiber assay, RAD51 foci kinetics, complementation

    PMID:25288561

    Open questions at the time
    • Mechanism linking HR defect to fork progression not defined
    • Origin-firing increase mechanism unresolved
  6. 2016 High

    Identified UAF1 as a stable partner, showing a SUMO-SIM interface builds a RAD51AP1-UAF1-RAD51 complex that enhances pairing and a separable role in regulating a later HR step and protein stability.

    Evidence In vitro synaptic complex/D-loop assays, SUMO-SIM mutagenesis, reciprocal Co-IP, RAD51 foci and stability assays

    PMID:27239033 PMID:27463890

    Open questions at the time
    • Relationship between USP1-independent pairing role and USP1-UAF1 deubiquitination role unclear at this stage
  7. 2019 High

    Demonstrated RAD51AP1's DNA-binding activity feeds into the Fanconi pathway by substituting for UAF1 DNA binding in FANCD2 deubiquitination.

    Evidence Reconstituted USP1-UAF1-RAD51AP1-FANCD2 deubiquitination assay, separation-of-function mutants, cellular assays

    PMID:31253762

    Open questions at the time
    • How DNA binding stimulates the catalytic deubiquitination step mechanistically not resolved
  8. 2019 High

    Established RAD51AP1 as essential for ALT telomere maintenance and showed its levels are controlled by MMS21-dependent SUMOylation, linking it to RAD52-POLD3 break-induced synthesis.

    Evidence ALT+ cell KO, telomere FISH, cGAS activation, SUMOylation assay, SUMO-site mutagenesis

    PMID:31400850

    Open questions at the time
    • Molecular substrate engaged at ALT telomeres not yet defined here
    • How SUMOylation alters activity unresolved
  9. 2021 High

    Showed RAD51AP1 binds nucleosomes, providing a mechanism to anchor chromatinized donor DNA to the RAD51 filament.

    Evidence In vitro NCP/octamer binding, joint-molecule assays with chromatinized DNA, domain mapping

    PMID:34058198

    Open questions at the time
    • In vivo contribution of nucleosome binding to HR not isolated
    • Histone-contact residues not pinpointed
  10. 2022 High

    Revealed an RNA-directed function at ALT telomeres: RAD51AP1 binds TERRA to build R-loops that generate G-quadruplexes and drive an R-to-D-loop switch enabling RAD52-independent break-induced replication.

    Evidence In vitro and cellular TERRA R-loop assays, S9.6 quantification, G4 detection, D-loop assays in RAD52 KO, SUMO-SIM mutants, proteomics

    PMID:36265486 PMID:36265488

    Open questions at the time
    • How the SUMO-SIM axis switches RAD51AP1 between DNA and RNA functions unresolved
    • G4-resolution step not defined
  11. 2022 Medium

    Defined RAD51AP1 and RAD54L as non-redundant HR sub-pathways downstream of RAD51 through synthetic genotoxic sensitivity.

    Evidence Double KO clonogenic survival with olaparib, MMC, hydroxyurea

    PMID:35652094

    Open questions at the time
    • Molecular divergence point between the two sub-pathways not mapped
    • Single-lab epistasis
  12. 2024 Medium

    Identified pre-rRNA as a binding partner that facilitates RAD51AP1 recruitment to DSBs and promotes its phase separation into foci.

    Evidence RIP, DSB colocalization imaging, Pol I inhibition, in vitro phase separation

    PMID:38403248

    Open questions at the time
    • Functional necessity of phase separation for HR not established
    • Single-lab finding
  13. 2024 Medium

    Placed RAD51AP1 in a transcriptionally driven drug-resistance circuit, with E2F1/H3K27ac/SOX9 inducing its expression to activate RAD51-UAF1 HR and confer temozolomide resistance in EGFRvIII glioblastoma.

    Evidence CRISPR synthetic-lethal screen, ChIP-seq/PCR, KD/OE HR and drug-sensitivity assays

    PMID:40211735

    Open questions at the time
    • Generality beyond EGFRvIII glioblastoma untested
    • Single-lab
  14. 2025 High

    Resolved the structural mechanism of RAD51 stimulation: cryo-EM showed RAD51AP1 uses three binding sites across two protomers to stabilize the filament interface and promote nucleation and strand exchange.

    Evidence Cryo-EM of RAD51-ssDNA filaments +/- RAD51AP1 in ATP and ADP states, biochemical strand-exchange and stabilization assays

    PMID:41337480

    Open questions at the time
    • Structure of RAD51AP1 DNA-binding domains on substrate not resolved
    • Meiotic DMC1 filament structure not addressed
  15. 2025 High

    Showed CDK2 phosphorylation at S277/S282 tunes RAD51AP1 binding avidity to ensure dynamic, productive cycling across consecutive HDR steps rather than maximal static binding.

    Evidence Phospho-deficient and phosphomimetic mutants, in vitro D-loop/EMSA, cellular toxicity and replication rescue, CDK2 identification

    PMID:41534830

    Open questions at the time
    • Spatiotemporal control of CDK2 phosphorylation during HR not mapped
    • Phosphatase reversing the mark unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RAD51AP1 integrates its competing DNA, RNA, nucleosome, and partner interactions into a single regulated switch — and how SUMOylation, phosphorylation, and SUMO-SIM signals are coordinated across mitotic HR, meiosis, and ALT telomere maintenance — remains unresolved.
  • No unified model coupling RNA-binding and DNA-binding modes
  • In vivo meiotic and ALT requirements not dissected by domain
  • Interplay of CDK2 and SUMO regulation undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 4 GO:0003723 RNA binding 3 GO:0098772 molecular function regulator activity 3 GO:0060090 molecular adaptor activity 2 GO:0042393 histone binding 1
Localization
GO:0000228 nuclear chromosome 3 GO:0005634 nucleus 2
Pathway
R-HSA-73894 DNA Repair 4 R-HSA-1474165 Reproduction 2 R-HSA-69306 DNA Replication 1
Partners
CDK2DMC1MMS21RAD51UAF1USP1
Complex memberships
RAD51AP1-UAF1 complexUSP1-UAF1-RAD51AP1 complex

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2007 RAD51AP1 binds both dsDNA and D-loop structures and, only when able to interact with RAD51, greatly stimulates the RAD51-mediated D-loop reaction. RAD51AP1 functions at a step subsequent to assembly of the RAD51-ssDNA nucleoprotein filament. RAD51AP1 is epistatic to the HR protein XRCC3. In vitro D-loop assay with purified RAD51AP1, EMSA for DNA binding, RNAi knockdown with HR repair assay and chromosomal break analysis, epistasis analysis with XRCC3 Molecular cell High 17996711
2007 RAD51AP1 is a structure-specific DNA binding protein with selective affinity for branched-DNA structures (obligatory intermediates during joint molecule formation) and stimulates RAD51-mediated joint molecule formation through the combination of structure-specific DNA binding and direct physical contact with RAD51. In vitro joint molecule formation assay, DNA binding assays (EMSA) with branched vs. linear substrates, Co-IP/physical interaction mapping, cellular DNA damage sensitivity assays Molecular cell High 17996710
2011 RAD51AP1 physically associates with the meiosis-specific recombinase DMC1 and stimulates the DMC1-mediated D-loop reaction by enhancing DMC1 presynaptic filament capacity to capture duplex-DNA and assemble the synaptic complex. Functional cooperation requires complex formation between DMC1 and RAD51AP1, and distinct epitopes in RAD51AP1 mediate interactions with RAD51 and DMC1. RAD51AP1 foci colocalize with a subset of DMC1 foci in mouse spermatocytes. In vitro D-loop assay with purified DMC1 and RAD51AP1, synaptic complex assembly assay, Co-IP, immunofluorescence in mouse spermatocytes Proceedings of the National Academy of Sciences of the United States of America High 21307306
2011 A highly conserved WVPP motif in RAD51AP1 is critical for DMC1 interaction but dispensable for RAD51 association, demonstrating that RAD51AP1 uses distinct epitopes to interact with RAD51 versus DMC1. This WVPP motif is reminiscent of the FVPP motif in BRCA2 that mediates DMC1 interaction. Series of truncation and point mutations in RAD51AP1 analyzed by Co-IP and interaction assays The Journal of biological chemistry High 21903585
2012 RAD51AP1 harbors two distinct DNA binding domains that are both required for maximal protein activity under physiological conditions; mutant variants impaired in either or both DNA binding domains are non-functional in cells. Domain mapping by truncation and point mutagenesis, EMSA, cellular complementation assays The Journal of biological chemistry High 22375013
2006 RAD51AP1/PIR51 and RAD51AP2 use the same conserved C-terminal structural motif (~57 residues in RAD51AP2, mapped to 40 aa in RAD51AP1) for RAD51 binding. Point mutations in this motif abolish RAD51 interaction. Yeast two-hybrid, truncation and point mutation analysis in HEK293 cells, Co-IP Nucleic acids research Medium 16990250
2016 UAF1 binds DNA and forms a dimeric complex with RAD51AP1 through SUMO-like domains in UAF1 and a SUMO-interacting motif (SIM) in RAD51AP1, and a trimeric complex with RAD51 through RAD51AP1. The RAD51AP1-UAF1 complex cooperates with RAD51 to assemble the synaptic complex and enhances RAD51-mediated homologous DNA pairing in a manner dependent on RAD51AP1 but independent of USP1. In vitro D-loop/synaptic complex assay with purified proteins, Co-IP, domain interaction mapping with SUMO-SIM mutations, cellular HR assay Cell reports High 27239033
2016 USP1-UAF1 complex interacts with RAD51AP1 via UAF1, which mediates the interaction; depletion of USP1 or UAF1 reduces RAD51AP1 stability. A UAF1 interaction-deficient mutant of RAD51AP1 causes persistent RAD51 foci following DNA damage, indicating RAD51AP1-UAF1 regulates a later step in HR repair. Proteomic pulldown of UAF1-interacting proteins, Co-IP, protein stability assays, RAD51 foci analysis after DNA damage, chromosomal aberration assay Cell cycle (Georgetown, Tex.) Medium 27463890
2019 Efficient FANCD2 deubiquitination by the USP1-UAF1 complex requires DNA and DNA binding by UAF1. The DNA binding activity of UAF1-associated RAD51AP1 can substitute for that of UAF1 in FANCD2 deubiquitination in a reconstituted biochemical system. DNA binding by UAF1 and RAD51AP1 is important for FANCD2 deubiquitination in cells. Reconstituted biochemical deubiquitination assay with purified USP1-UAF1-RAD51AP1 and FANCD2, cellular deubiquitination assay with separation-of-function mutants Nature communications High 31253762
2019 RAD51AP1 is required for both RAD51-dependent HR and RAD52-POLD3-dependent break-induced DNA synthesis at ALT telomeres. RAD51AP1 KO in ALT+ cells causes generational telomere shortening, telomere dysfunction, and cytosolic telomeric DNA that activates cGAS. RAD51AP1 protein levels are elevated in ALT+ cells due to MMS21-associated SUMOylation; mutation of a single SUMO-targeted lysine residue perturbs telomere dynamics. RAD51AP1 KO in ALT+ cancer cells, telomere FISH, cGAS activation assay, autophagy assays, SUMOylation assay with MMS21, SUMO-site mutagenesis Molecular cell High 31400850
2014 RAD51AP1 deficiency in vertebrate cells impairs DNA replication fork progression and causes increased replication origin firing. In RAD51AP1-deficient cells, resolution of DNA damage-induced RAD51 foci is greatly slowed while their formation is not impaired, placing RAD51AP1 function after RAD51 filament assembly. Targeted RAD51AP1 gene inactivation in chicken DT40 cells, DNA fiber assay for replication fork speed, RAD51 foci kinetics by immunofluorescence, complementation with human/chicken RAD51AP1 DNA repair High 25288561
2021 RAD51AP1 binds to nucleosome core particles (NCPs) through a C-terminal region including its DNA-binding domain, and can promote duplex DNA capture and joint-molecule formation with chromatinized template DNA in vitro, suggesting RAD51AP1 anchors the DNA template through nucleosome affinity to the RAD51-ssDNA filament. In vitro binding assays (EMSA, pulldown) with purified NCPs and histone octamers, in vitro D-loop/joint molecule assay with chromatinized DNA, domain mapping by truncation The Journal of biological chemistry High 34058198
2022 RAD51AP1 interacts with TERRA RNA and uses it to generate D-loop and R-loop HR intermediates at telomeres. RAD51AP1 depletion reduces R-loop formation at telomere DNA breaks. RAD51AP1-mediated TERRA R-loop homeostasis suppresses TERRA transcription and prevents transcription-replication collisions during ALT-HDR. Both TERRA binding and this chromatin-directed function require RAD51AP1's intrinsic SUMO-SIM regulatory axis. TERRA R-loop assays in vitro and at telomeres, RAD51AP1 KD with R-loop measurement (S9.6 antibody), proteomics of RAD51AP1-associated proteins, SUMO-SIM mutant analysis Molecular cell High 36265488
2022 TERRA forms R-loops in vitro and at telomeres in a RAD51AP1-dependent manner. RAD51AP1-assembled TERRA R-loops generate G-quadruplexes (G4s) at telomeres, which persist after R-loop resolution and enable D-loop formation without RAD52, orchestrating an R-to-D-loop switch that promotes break-induced replication in the RAD52-independent ALT pathway. In vitro R-loop formation assay with purified TERRA and RAD51AP1, telomeric R-loop quantification in RAD51AP1-dependent knockdown cells, G4 detection, D-loop assay in RAD52 KO cells Molecular cell High 36265486
2022 RAD51AP1 and RAD54L define two distinct sub-pathways downstream of RAD51 in HR; simultaneous deletion of RAD51AP1 and RAD54L shows synthetic sensitivity to PARP inhibitor (olaparib), mitomycin C, and hydroxyurea, establishing that the two proteins underpin non-redundant HR routes. Double KO of RAD51AP1 and RAD54L in human cancer cell lines, clonogenic survival with olaparib, MMC, hydroxyurea; comparison with RAD54B single KO Frontiers in cell and developmental biology Medium 35652094
2024 RAD51AP1 associates with pre-rRNA through both its N-terminus and C-terminus. Pre-rRNA colocalizes with RAD51AP1 at double-strand breaks and facilitates RAD51AP1 recruitment to DSBs. RAD51AP1 forms liquid-liquid phase separation in the presence of pre-rRNA in vitro, which may underlie RAD51AP1 foci formation. RNA immunoprecipitation, colocalization at DSBs by imaging, RNA polymerase I inhibitor treatment, in vitro phase separation assay with purified RAD51AP1 and pre-rRNA The Journal of biological chemistry Medium 38403248
2025 RAD51AP1 possesses at least three RAD51-binding sites that span two adjacent RAD51 molecules. RAD51AP1 stabilizes the RAD51 N-terminal domain and protomer interface in filaments, promotes RAD51-ssDNA filament nucleation and stabilization, and stimulates strand exchange. Structural data show RAD51AP1 binding induces conformational changes promoting RAD51 DNA association and oligomerization. RAD51-ssDNA filaments expand upon ATP hydrolysis to ADP, explaining reduced RAD51-DNA binding in the ADP state. Cryo-EM structures of RAD51-ssDNA filaments with and without RAD51AP1 in Mg2+-ATP and Mg2+-ADP states, biochemical strand exchange assays, filament stabilization assays Proceedings of the National Academy of Sciences of the United States of America High 41337480
2025 RAD51AP1 activity in HDR is regulated by CDK2-mediated phosphorylation at S277 and S282. A phospho-deficient S277/282A mutant more avidly binds ssDNA, dsDNA, and nucleosome core particles and is more proficient in D-loop stimulation in vitro, but fails to rescue RAD51AP1 deficiency in cellular toxicity and DNA replication assays. A phosphomimetic S277/282D mutant fully rescues RAD51AP1 deficiency in cells, suggesting phosphorylation ensures dynamic RAD51AP1 engagement across consecutive HDR steps. Site-directed mutagenesis (S277A, S282A, S277/282D), in vitro D-loop assay, EMSA with ssDNA/dsDNA/NCP, cellular toxicity and DNA replication assays, CDK2 phosphorylation identification The Journal of biological chemistry High 41534830
2025 RAD51AP1 is required for TERRA R-loop formation in telomerase-expressing cells; both RAD51 and RAD51AP1 contribute non-redundant functions to TERRA R-loop formation. TERRA R-loops interfere with semiconservative DNA replication and promote break-induced replication for telomere maintenance. TERRA overexpression in telomerase+ cells, RAD51AP1 depletion, R-loop measurement, DNA replication interference assay, BIR assay bioRxivpreprint Medium bio_10.1101_2025.01.09.632133
2024 E2F1 transcription factor drives RAD51AP1 expression in EGFRvIII-positive glioblastoma cells in response to TMZ treatment; RAD51AP1 promotes formation of the RAD51-UAF1 complex to activate homologous recombination and thereby confers TMZ resistance. H3K27ac and SOX9 together induce RAD51AP1 transcription in EGFRvIII cells. CRISPR/Cas9 synthetic lethal screen, ChIP-seq and ChIP-PCR for H3K27ac and SOX9 at RAD51AP1 promoter, RAD51AP1 KD and OE with HR and drug sensitivity assays Chinese medical journal Medium 40211735

Source papers

Stage 0 corpus · 46 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 Promotion of homologous recombination and genomic stability by RAD51AP1 via RAD51 recombinase enhancement. Molecular cell 121 17996711
2007 RAD51AP1 is a structure-specific DNA binding protein that stimulates joint molecule formation during RAD51-mediated homologous recombination. Molecular cell 100 17996710
2022 TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch. Molecular cell 92 36265486
2004 Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma. World journal of gastroenterology 59 14966907
2019 RAD51AP1 Is an Essential Mediator of Alternative Lengthening of Telomeres. Molecular cell 56 31400850
2019 DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nature communications 55 31253762
2022 RAD51AP1 regulates ALT-HDR through chromatin-directed homeostasis of TERRA. Molecular cell 54 36265488
2015 NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic acids research 54 26323318
2016 Promotion of RAD51-Mediated Homologous DNA Pairing by the RAD51AP1-UAF1 Complex. Cell reports 51 27239033
2017 Role of RAD51AP1 in homologous recombination DNA repair and carcinogenesis. DNA repair 46 28963981
2012 Mechanistic insights into RAD51-associated protein 1 (RAD51AP1) action in homologous DNA repair. The Journal of biological chemistry 40 22375013
2011 Molecular basis for enhancement of the meiotic DMC1 recombinase by RAD51 associated protein 1 (RAD51AP1). Proceedings of the National Academy of Sciences of the United States of America 37 21307306
2006 Pir51, a Rad51-interacting protein with high expression in aggressive lymphoma, controls mitomycin C sensitivity and prevents chromosomal breaks. Mutation research 35 16920159
2016 The USP1-UAF1 complex interacts with RAD51AP1 to promote homologous recombination repair. Cell cycle (Georgetown, Tex.) 31 27463890
2020 RAD51AP1 Deficiency Reduces Tumor Growth by Targeting Stem Cell Self-Renewal. Cancer research 29 32665355
2020 RAD51AP1 promotes progression of ovarian cancer via TGF-β/Smad signalling pathway. Journal of cellular and molecular medicine 27 33314567
2014 RAD51AP1-deficiency in vertebrate cells impairs DNA replication. DNA repair 27 25288561
2022 RAD51AP1 and RAD54L Can Underpin Two Distinct RAD51-Dependent Routes of DNA Damage Repair via Homologous Recombination. Frontiers in cell and developmental biology 26 35652094
2006 RAD51AP2, a novel vertebrate- and meiotic-specific protein, shares a conserved RAD51-interacting C-terminal domain with RAD51AP1/PIR51. Nucleic acids research 26 16990250
2011 RAD51-associated protein 1 (RAD51AP1) interacts with the meiotic recombinase DMC1 through a conserved motif. The Journal of biological chemistry 23 21903585
2021 RAD51AP1 Loss Attenuates Colorectal Cancer Stem Cell Renewal and Sensitizes to Chemotherapy. Molecular cancer research : MCR 20 34099522
2019 Silencing of RAD51AP1 suppresses epithelial-mesenchymal transition and metastasis in non-small cell lung cancer. Thoracic cancer 19 31317661
2019 Single-cell RNA-seq reveals RAD51AP1 as a potent mediator of EGFRvIII in human glioblastomas. Aging 19 31532757
2023 Identification of the E2F1-RAD51AP1 axis as a key factor in MGMT-methylated GBM TMZ resistance. Cancer biology & medicine 17 37283490
2020 A Novel Neoplastic Fusion Transcript, RAD51AP1-DYRK4, Confers Sensitivity to the MEK Inhibitor Trametinib in Aggressive Breast Cancers. Clinical cancer research : an official journal of the American Association for Cancer Research 16 33172895
2021 RAD51AP1 mediates RAD51 activity through nucleosome interaction. The Journal of biological chemistry 12 34058198
2021 ZEB1 induces non-small cell lung cancer development by targeting microRNA-320a to increase the expression of RAD51AP1. Experimental cell research 10 34097859
2008 Germline mutations in RAD51, RAD51AP1, RAD51B, RAD51C,RAD51D, RAD52 and RAD54L do not contribute to familial chronic lymphocytic leukemia. Leukemia & lymphoma 9 18203022
2024 Identification of RAD51AP1 as a key gene in hepatitis B virus-associated hepatocellular carcinoma. Heliyon 8 39850418
2024 RRM1 promotes homologous recombination and radio/chemo-sensitivity via enhancing USP11 and E2F1-mediated RAD51AP1 transcription. Cell death discovery 6 39695160
2023 miR-383-5p Regulates Preadipocyte Proliferation and Differentiation by Targeting RAD51AP1. International journal of molecular sciences 6 37762324
2022 Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro. Journal of personalized medicine 6 35207688
2024 Pre-rRNA facilitates the recruitment of RAD51AP1 to DNA double-strand breaks. The Journal of biological chemistry 5 38403248
2022 Knockdown of RAD51AP1 suppressed cell proliferation and invasion in esophageal squamous cell carcinoma. Discover oncology 5 36197550
2025 Epigenetically elevated RAD51AP1 regulates the RAD51-UAF1 complex contributing to temozolomide resistance in EGFRvIII glioblastoma. Chinese medical journal 3 40211735
2023 E2F7/RAD51AP1 Axis Inhibits Endometrial Cancer Sensitivity to 5-FU via the Fatty Acid Metabolic Pathway. Anticancer research 2 37909953
2023 The gene RAD51AP1 promotes the progression of pancreatic cancer via the PI3K/Akt/NF-κB signaling pathway. Neoplasma 2 37962862
2023 [Research Advances of RAD51AP1 in Tumor Progression and Drug Resistance]. Zhongguo fei ai za zhi = Chinese journal of lung cancer 2 37985156
2022 TERRA and RAD51AP1 at the R&D-loop department of ALT telomeres. Molecular cell 2 36332602
2025 RAD51AP1 is a versatile RAD51 modulator. Proceedings of the National Academy of Sciences of the United States of America 1 41337480
2024 Knocking down RAD51AP1 enhances chemosensitivity by inhibiting the self-renewal of CD133 positive ovarian cancer stem-like cells. Discover oncology 1 39235706
2026 Phosphoregulation of RAD51AP1 function in homology-directed repair. The Journal of biological chemistry 0 41534830
2026 DLGAP5 protects glioblastoma cells against DNA damage through E2F1-transcripted RAD51AP1. Biochimica et biophysica acta. Molecular basis of disease 0 42002087
2025 Transcriptomic analysis of laser-capture microdissected tumors reveals RAD51AP1 as a tumor-specific marker associated progression from pancreatic intraepithelial neoplasia to invasive pancreatic cancer. Cancer cell international 0 40495122
2025 Phosphoregulation of RAD51AP1 function in homology-directed repair. bioRxiv : the preprint server for biology 0 40964358
2025 Research Progress of RAD51AP1 in Malignant Tumors of the Female Reproductive System. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 0 41109978

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