Affinage

NUCKS1

Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 · UniProt Q9H1E3

Length
243 aa
Mass
27.3 kDa
Annotated
2026-06-10
50 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NUCKS1 is a vertebrate-specific, chromatin-associated nuclear phosphoprotein that couples mitogenic and DNA-damage signaling to cell-cycle control through dual roles as a chromatin-binding transcriptional regulator and a homologous-recombination repair factor (PMID:24931609, PMID:26323318, PMID:34845229). Its DNA-binding activity is mediated by a basic GRP-motif domain that inserts into the DNA major groove, and a Cdk1 phosphorylation site within this domain switches DNA binding off, linking its chromatin engagement to cell-cycle kinase activity (PMID:12413487). Nuclear import depends on a bipartite NLS that engages importin alpha3/alpha5 (PMID:17604136), and the protein is one of the most heavily post-translationally modified proteins known, carrying numerous phosphorylation, acetylation, methylation and formylation sites with cell-cycle-dependent dynamics (PMID:18491381); phosphorylation is imposed by CK2, CDK1, and the DNA-damage kinases ATM and DNA-PK (PMID:28011258). As a transcriptional activator recruited to chromatin upon mitogenic stimulation, NUCKS1 activates SKP2 to drive p21/p27 degradation and S-phase entry, a circuit that is reversed by p53-dependent transcriptional repression of NUCKS1 following DNA damage (the NUCKS1-SKP2-p21/p27 axis) (PMID:34845229). In DNA repair, NUCKS1 acts as a RAD51AP1 paralog that binds DNA and stimulates RAD54 ATPase activity and RAD51-RAD54-mediated strand invasion during D-loop formation, promoting homologous recombination, replication fork progression, and suppression of radiation-induced lymphomagenesis (PMID:26323318, PMID:32876692, PMID:27542204). NUCKS1 also functions as a transcriptional activator of insulin/IGF signaling components, and Nucks1-knockout mice display impaired insulin signaling, obesity, glucose intolerance and insulin resistance, with hypothalamic NUCKS1 governing peripheral glucose homeostasis (PMID:24931609, PMID:26205492). Across multiple cancers NUCKS1 promotes proliferation, invasion and metastasis by transcriptionally upregulating target genes including IGF-1R, ASNS, CXCL8, Cdc42 and S100A9 (PMID:30371738, PMID:37528150, PMID:38636892, PMID:40226459, PMID:40946978).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2001 High

    Established NUCKS1 as a bona fide nuclear phosphoprotein and Cdk1 substrate, defining the first molecular handle on a cell-cycle-linked function.

    Evidence Phosphopeptide mapping and back-phosphorylation of HeLa interphase versus metaphase protein

    PMID:11298763

    Open questions at the time
    • Functional consequence of mitotic phosphorylation not defined
    • No DNA-binding or transcriptional role established yet
  2. 2002 Medium

    Identified the DNA-binding determinant and showed it is regulated by Cdk1 phosphorylation, connecting kinase modification to chromatin engagement.

    Evidence Synthetic peptide DNA-binding assays, NMR, docking, and in vitro phosphorylation

    PMID:12413487

    Open questions at the time
    • In vitro peptide model rather than full-length protein in cells
    • Sequence specificity of DNA binding not resolved
  3. 2004 Medium

    Demonstrated vertebrate conservation of NUCKS1 as a CDK1/CK2 substrate and characterized its promoter architecture.

    Evidence In vitro kinase assays on fish ortholog, immunoblotting, database analysis

    PMID:15381070

    Open questions at the time
    • No invertebrate ortholog characterized
    • Functional role still undefined
  4. 2007 High

    Defined the nuclear import mechanism, showing a bipartite NLS drives receptor-mediated import via importin alpha3/alpha5.

    Evidence GFP-fusion localization, mutagenesis, pull-down, splice-variant analysis

    PMID:17604136

    Open questions at the time
    • Regulation of import during cell cycle not addressed
    • Function of NLS2 not fully resolved
  5. 2008 High

    Mapped the extraordinary PTM landscape of NUCKS1 with cell-cycle-dependent dynamics, framing it as a heavily regulated signaling hub.

    Evidence SILAC quantitative proteomics and mass spectrometry across cell-cycle states

    PMID:18491381

    Open questions at the time
    • Functional role of most individual PTMs unknown
    • Responsible enzymes for many sites unidentified
  6. 2014 High

    Revealed NUCKS1 as a transcriptional activator of insulin-signaling genes with a defined whole-animal metabolic phenotype.

    Evidence ChIP-seq plus Nucks1-knockout mice with glucose/insulin tolerance testing

    PMID:24931609

    Open questions at the time
    • Direct DNA-binding specificity of NUCKS1 at these promoters not resolved
    • Cofactors mediating Pol II recruitment unknown
  7. 2014 Medium

    Showed NUCKS1 acts as a host coactivator of HIV-1 Tat, extending its transcriptional regulatory role to viral chromatin.

    Evidence Yeast two-hybrid, reciprocal co-IP, ChIP at the HIV-1 LTR, reporter and knockdown assays

    PMID:25116364

    Open questions at the time
    • Single lab, mechanism of Tat stabilization at TAR unresolved
    • Direct versus indirect chromatin recruitment unclear
  8. 2015 High

    Established NUCKS1 as a RAD51AP1 paralog functioning in homologous recombination and replication, defining its genome-stability role.

    Evidence siRNA knockdown HR assays, DNA fiber, MMC sensitivity, recombinant DNA-binding assays

    PMID:26323318

    Open questions at the time
    • Mechanistic step within HR not yet defined
    • Lower DNA-binding affinity than RAD51AP1 implies distinct mode
  9. 2015 High

    Localized the metabolic function to hypothalamic NUCKS1, showing tissue specificity of its insulin/glucose control.

    Evidence Conditional Nkx2.1-Cre and immune-cell-specific knockouts with metabolic phenotyping

    PMID:26205492

    Open questions at the time
    • Hypothalamic neuronal target genes not mapped
    • Link to central insulin action mechanistically incomplete
  10. 2016 High

    Identified the kinases imposing NUCKS1 phosphorylation and linked ATM/DNA-PK modification to the DNA-damage response.

    Evidence In vitro/in vivo kinase assays, MS, phosphospecific antibodies, inhibitors, irradiation

    PMID:28011258

    Open questions at the time
    • Functional consequence of ATM phosphorylation on HR not directly shown
    • Downstream effectors of CK2 sites unknown
  11. 2016 Medium

    Provided in vivo genetic evidence that NUCKS1 suppresses radiation-induced tumorigenesis, consistent with a DNA-damage-response tumor-suppressor role.

    Evidence Trp53+/- Nucks1+/- compound mutant mice, irradiation, tumor and LOH analysis

    PMID:27542204

    Open questions at the time
    • Single lab haploinsufficiency model
    • Mechanistic link to HR defect in vivo not established
  12. 2017 Medium

    Extended NUCKS1 function to NF-kB-driven inflammatory cytokine expression in an in vivo injury model.

    Evidence Nucks1-knockout mice, corneal alkali burn, LPS-NF-kB assays, cytokine profiling

    PMID:28106169

    Open questions at the time
    • Direct versus indirect effect on NF-kB unclear
    • No chromatin-level mechanism at cytokine promoters
  13. 2020 High

    Resolved the biochemical step NUCKS1 performs in HR, showing it stimulates RAD54 ATPase and RAD51-RAD54 strand invasion.

    Evidence Co-IP, reconstituted ATPase and D-loop assays, focus resolution in cells

    PMID:32876692

    Open questions at the time
    • Structural basis of RAD54 stimulation not determined
    • Regulation of the NUCKS1/RAD51AP1 partner choice unclear
  14. 2021 High

    Defined the NUCKS1-SKP2-p21/p27 axis integrating mitogenic and p53-dependent DNA-damage signals at the G1/S transition.

    Evidence ChIP, knockdown, reporter assays, cell-cycle analysis, multi-component epistasis

    PMID:34845229

    Open questions at the time
    • DNA sequence motif bound by NUCKS1 at SKP2 not defined
    • Coactivator machinery at SKP2 promoter unknown
  15. 2025 Medium

    Showed that NUCKS1 drives diverse oncogenic programs by transcriptionally upregulating effector genes and stabilizing protumorigenic proteins across cancer types.

    Evidence ChIP/promoter reporter and epistasis for IGF-1R, ASNS, CXCL8, Cdc42, S100A9, plus HDAC2 stabilization; xenograft and metastasis models

    PMID:30371738 PMID:37528150 PMID:38636892 PMID:40226459 PMID:40527876 PMID:40946978

    Open questions at the time
    • Each target characterized in a single tumor context by a single lab
    • Direct DNA-binding specificity across these promoters not unified
    • Mechanism of protein stabilization (HDAC2, via NUCB2) incompletely defined
  16. 2026 Medium

    Placed NUCKS1 downstream of a HERC2-DDX21 module that controls its transcription to govern cancer-cell dormancy via p21/p27.

    Evidence Proteomics, ChIP of DDX21 at the NUCKS1 promoter, ubiquitination assays, cell-cycle analysis

    PMID:42071007

    Open questions at the time
    • Single lab
    • Generality beyond dormancy-like colorectal cells unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NUCKS1's chromatin-binding mode achieves promoter selectivity, and how its many PTMs coordinate the switch between transcriptional regulation and HR repair, remain unresolved.
  • No consensus DNA-binding motif defined despite many direct promoter targets
  • No structural model of NUCKS1 on chromatin or in the RAD54 complex
  • PTM code that toggles transcription versus repair function not deciphered

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 3 GO:0003677 DNA binding 2 GO:0098772 molecular function regulator activity 1
Localization
GO:0005634 nucleus 2 GO:0005694 chromosome 2
Pathway
R-HSA-162582 Signal Transduction 2 R-HSA-73894 DNA Repair 2 R-HSA-74160 Gene expression (Transcription) 2 R-HSA-1640170 Cell Cycle 1
Partners
CDK1IMPORTIN ALPHA3IMPORTIN ALPHA5NUCB2RAD51AP1RAD54TAT (HIV-1)

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 NUCKS1 (then called P1/NUCKS) is a nuclear phosphoprotein that serves as a substrate for Cdk1 during mitosis; phosphopeptide mapping and back-phosphorylation experiments using HeLa interphase and metaphase cell-derived protein demonstrated mitosis-specific phosphorylation of threonine residues by Cdk1. Phosphopeptide mapping, back-phosphorylation assays, molecular cloning European journal of biochemistry High 11298763
2002 NUCKS1 contains a putative DNA-binding domain with an extended GRP motif that forms an alpha helix and fits into the major groove of DNA via basic residues; phosphorylation of the Cdk1 site within this domain completely abolishes DNA binding in vitro. Synthetic peptide DNA-binding assay, NMR structural analysis, docking modeling, in vitro phosphorylation Archives of biochemistry and biophysics Medium 12413487
2004 The NUCKS1 protein is a vertebrate-specific protein whose fish ortholog is also a substrate for CDK1 and CK-2 in vitro, indicating conserved kinase substrate function across vertebrates. The gene lacks a TATA box but contains Inr elements, GC boxes, and an E2F-1 binding site. In vitro kinase assay, Western immunoblotting, database searches Biochemical and biophysical research communications Medium 15381070
2007 NUCKS1 contains two functional nuclear localization signals (NLS1 and NLS2); NLS1 is the primary bipartite NLS and is required for nuclear import. NLS1 mediates binding to importin alpha3 and importin alpha5 in vitro, indicating receptor-mediated nuclear import. A splice variant lacking NLS1 fails to enter the nucleus. GFP-fusion subcellular localization, site-directed mutagenesis, GFP-immunoprecipitation, GST pull-down, immunofluorescence Biochimica et biophysica acta High 17604136
2008 NUCKS1 is one of the most heavily post-translationally modified proteins known; SILAC-based quantitative proteomics identified 25 phosphorylation sites (6 novel), plus multiple acetylation, methylation, and formylation sites. Cell-cycle-dependent changes include increased phosphorylation and decreased acetylation in mitotically arrested cells. At least 36 of 243 residues can be modified (57 PTMs total). SILAC quantitative proteomics, mass spectrometry, phosphopeptide mapping Proteins High 18491381
2014 NUCKS1 functions as a transcriptional activator of the insulin receptor (IR) and other insulin signaling components by regulating chromatin accessibility and RNA Pol II recruitment to their promoters. NUCKS1 knockout mice show impaired insulin signaling, obesity, glucose intolerance and insulin resistance, phenotypes worsened by high-fat diet. Genome-wide ChIP-seq, NUCKS1 knockout mouse, glucose/insulin tolerance tests, knockdown in endocrine cells Cell reports High 24931609
2014 NUCKS1 physically interacts with HIV-1 Tat protein (identified by yeast two-hybrid and confirmed by co-immunoprecipitation) and acts as a Tat coactivator; NUCKS1 knockdown reduces Tat accumulation at the TAR region of the HIV-1 LTR (by ChIP assay) and diminishes viral transcription and replication, without affecting Tat nuclear localization or Cyclin T1 interaction. Yeast two-hybrid, co-immunoprecipitation, chromatin immunoprecipitation (ChIP), reporter assay, siRNA knockdown Retrovirology Medium 25116364
2015 NUCKS1 shares extensive sequence homology with RAD51AP1 and functions as a paralog in homologous recombination (HR) DNA repair; NUCKS1 depletion impairs HR, increases sensitivity to mitomycin C, increases chromatid breaks, and slows replication fork progression with increased new origin firing. Recombinant NUCKS1 binds DNA with the same preference as RAD51AP1 but with lower affinity. siRNA knockdown, HR assay, chromatid break analysis, DNA fiber assay, recombinant protein DNA-binding assay, MMC sensitivity assay Nucleic acids research High 26323318
2015 Hypothalamic NUCKS1 specifically regulates peripheral glucose homeostasis and insulin signaling; hypothalamus-specific NUCKS1 knockout mice show glucose intolerance on normal chow and exacerbated obesity and insulin resistance on high-fat diet, while immune-cell-specific NUCKS1 knockout does not produce these phenotypes. Conditional tissue-specific Cre-lox knockout mice (Nkx2.1-Cre), glucose tolerance test, insulin tolerance test, intracerebroventricular insulin injection The Biochemical journal High 26205492
2016 NUCKS1 is phosphorylated on 11 sites by CK2 (at least 7 confirmed in vivo) and on two sites by ATM kinase and DNA-PK in vitro; ATM-mediated phosphorylation of NUCKS1 occurs in vivo in gamma-irradiated cells, linking NUCKS1 to the DNA damage response via PIKK kinases. In vitro and in vivo phosphorylation assays, mass spectrometry, phosphopeptide mapping, phosphoamino acid analysis, phosphospecific antibodies, kinase inhibitors Biochimica et biophysica acta. Proteins and proteomics High 28011258
2016 In a Trp53-deficient background, loss of one Nucks1 allele accelerates radiation-induced thymic lymphoma development in mice, and wild-type Nucks1 levels are required to suppress radiation-induced lymphomagenesis, consistent with NUCKS1 functioning in the DNA damage response as a tumor suppressor. Mouse genetics (Trp53+/- Nucks1+/- compound mutant), X-irradiation, tumor analysis, LOH analysis, flow cytometry Oncotarget Medium 27542204
2017 NUCKS1 regulates NF-κB-mediated cytokine expression in corneal epithelial cells; NUCKS1 knockout reduces LPS-induced NF-κB activation and downstream expression of IL6, IP10, and TNFα in vitro, and suppresses inflammation and neovascularization in an in vivo corneal alkali burn model. NUCKS1 knockout mice, alkali burn model, in vitro LPS-NF-κB assay, cytokine profiling, siRNA silencing Scientific reports Medium 28106169
2019 NUCKS1 promotes gastric cancer cell proliferation and invasion via transcriptional upregulation of IGF-1R, which activates the PI3K/Akt/mTOR pathway; IGF-1R knockdown eliminates the pro-tumorigenic effects of NUCKS1 overexpression, placing NUCKS1 upstream of IGF-1R/PI3K/Akt/mTOR signaling. siRNA knockdown, ectopic overexpression, xenograft mouse model, Western blotting, cell invasion/proliferation assays Carcinogenesis Medium 30371738
2020 NUCKS1 physically and functionally interacts with the DNA motor protein RAD54; NUCKS1 stimulates the ATPase activity of RAD54 and the RAD51-RAD54-mediated strand invasion step during D-loop formation in vitro. In cells, NUCKS1 controls resolution of RAD54 foci after DNA damage and prevents inappropriate engagement of RAD54 with RAD51AP1 in unperturbed cells. Co-immunoprecipitation, in vitro ATPase assay, in vitro D-loop assay, focus formation/resolution assay, cell-based DNA damage assays The Journal of cell biology High 32876692
2020 NUCKS1 knockdown in gastric cancer cells induces autophagy through the mTOR-Beclin1 pathway; this autophagy induction is rescued by NUCKS1 restoration, and Beclin1 silencing in NUCKS1-knockdown cells, or rapamycin treatment in NUCKS1-overexpressing cells, confirms NUCKS1 acts upstream of mTOR to suppress Beclin1-dependent autophagy. siRNA knockdown, genetic rescue, rapamycin treatment, Western blotting, autophagy assays, xenograft Journal of experimental & clinical cancer research Medium 32958058
2020 NUCKS1 physically interacts with CDK1 (verified by co-immunoprecipitation) and promotes CDK1 expression in NSCLC cells; CDK1 knockdown or inhibition alleviates the pro-proliferative and pro-invasive effects of NUCKS1 overexpression. Co-immunoprecipitation, shRNA knockdown, CDK1 inhibitor, cell proliferation/invasion assays, xenograft Cancer management and research Medium 33380837
2021 NUCKS1 functions as a transcription factor that is recruited to chromatin in response to mitogenic stimulation to activate SKP2 expression, leading to degradation of p21 and p27 and S phase entry. DNA damage induces p53-dependent transcriptional repression of NUCKS1, causing SKP2 downregulation, p21/p27 accumulation, and cell cycle arrest (NUCKS1-SKP2-p21/p27 axis). ChIP, siRNA/shRNA knockdown, reporter assay, cell cycle analysis, epistasis by genetic manipulation of each component Nature communications High 34845229
2023 NUCKS1 transcriptionally upregulates asparagine synthetase (ASNS), elevating asparagine levels in osteosarcoma cells to promote proliferation and metastasis; ASNS inhibition or asparagine reduction diminishes the pro-tumorigenic effects of NUCKS1. siRNA knockdown, overexpression, asparagine measurement, ASNS inhibition, xenograft, in vitro functional assays Cell death & disease Medium 37528150
2024 NUCB2 interacts with NUCKS1 protein to inhibit its degradation (stabilization), which in turn allows NUCKS1 to transcriptionally upregulate CXCL8 expression in osteosarcoma, promoting PD-L1 expression and immunosuppression. Co-immunoprecipitation, knockdown, Western blotting, reporter assay, in vivo tumor model Cancer letters Medium 38636892
2025 NUCKS1 directly binds to the promoter of Cdc42 and transcriptionally upregulates Cdc42 expression in hepatocellular carcinoma, promoting cancer cell proliferation and migration; Cdc42 expression is positively correlated with NUCKS1 in HCC tissues. ChIP (promoter binding), siRNA knockdown, overexpression, in vitro and in vivo functional assays American journal of cancer research Medium 40226459
2025 NUCKS1 stabilizes HDAC2 by inhibiting lysosomal degradation, leading to AKT activation and promotion of colorectal cancer invasion and metastasis; HDAC2 inhibition or AKT inhibition rescues the pro-metastatic effects of NUCKS1 overexpression both in vitro and in vivo. Knockdown/overexpression, HDAC2 inhibitor (Santacruzamate A), AKT inhibitor (LY294002), in vivo tail-vein metastasis model, Western blotting Oncogenesis Medium 40527876
2025 NUCKS1 directly binds to the promoter of S100A9 and transcriptionally upregulates its expression in HNSCC, enhancing malignancy; NUCKS1 ablation decreases S100A9 promoter activity and expression. Promoter reporter assay, siRNA knockdown, overexpression, in vitro and in vivo functional assays Biochemical pharmacology Medium 40946978
2026 DDX21 binds the NUCKS1 promoter and its downregulation reduces NUCKS1 transcription, leading to elevated p21/p27 levels and G0/G1 arrest in dormancy-like colorectal cancer cells; HERC2 mediates K48/K63-linked polyubiquitination and degradation of DDX21, placing DDX21 upstream of NUCKS1 in a HERC2-DDX21-NUCKS1-p21/p27 axis. Proteomics, ChIP (DDX21 binding to NUCKS1 promoter), ubiquitination assay, knockdown/overexpression, cell cycle analysis Cell death & disease Medium 42071007

Source papers

Stage 0 corpus · 50 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Molecular cloning of a mammalian nuclear phosphoprotein NUCKS, which serves as a substrate for Cdk1 in vivo. European journal of biochemistry 66 11298763
2021 The NUCKS1-SKP2-p21/p27 axis controls S phase entry. Nature communications 63 34845229
2015 NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic acids research 54 26323318
2019 NUCKS1 promotes gastric cancer cell aggressiveness by upregulating IGF-1R and subsequently activating the PI3K/Akt/mTOR signaling pathway. Carcinogenesis 47 30371738
2008 Constitutive and dynamic phosphorylation and acetylation sites on NUCKS, a hypermodified nuclear protein, studied by quantitative proteomics. Proteins 39 18491381
2014 NUCKS is a positive transcriptional regulator of insulin signaling. Cell reports 37 24931609
2004 Characterisation of the NUCKS gene on human chromosome 1q32.1 and the presence of a homologous gene in different species. Biochemical and biophysical research communications 37 15381070
2009 NUCKS overexpression in breast cancer. Cancer cell international 33 19664271
2020 NUCKS1 Promotes Proliferation, Invasion and Migration of Non-Small Cell Lung Cancer by Upregulating CDK1 Expression. Cancer management and research 31 33380837
2014 NUCKS1 overexpression is a novel biomarker for recurrence-free survival in cervical squamous cell carcinoma. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 30 24819170
2020 NUCKS promotes cell proliferation and suppresses autophagy through the mTOR-Beclin1 pathway in gastric cancer. Journal of experimental & clinical cancer research : CR 28 32958058
2020 NUCKS1 promotes RAD54 activity in homologous recombination DNA repair. The Journal of cell biology 27 32876692
2007 Identification and characterization of two putative nuclear localization signals (NLS) in the DNA-binding protein NUCKS. Biochimica et biophysica acta 27 17604136
2022 miR-641 Inhibited Cell Proliferation and Induced Apoptosis by Targeting NUCKS1/PI3K/AKT Signaling Pathway in Breast Cancer. Computational and mathematical methods in medicine 23 35069784
2018 Roles of NUCKS1 in Diseases: Susceptibility, Potential Biomarker, and Regulatory Mechanisms. BioMed research international 23 29619377
2017 Inhibition of NUCKS Facilitates Corneal Recovery Following Alkali Burn. Scientific reports 23 28106169
2016 Identification of NUCKS1 as a putative oncogene and immunodiagnostic marker of hepatocellular carcinoma. Gene 23 26968889
2002 A putative DNA-binding domain in the NUCKS protein. Archives of biochemistry and biophysics 23 12413487
2019 NUCKS1 is a novel regulator of milk synthesis in and proliferation of mammary epithelial cells via the mTOR signaling pathway. Journal of cellular physiology 21 30710349
2014 NUCKS1, a novel Tat coactivator, plays a crucial role in HIV-1 replication by increasing Tat-mediated viral transcription on the HIV-1 LTR promoter. Retrovirology 21 25116364
2015 NUCKS: a potential biomarker in cancer and metabolic disease. Clinical science (London, England : 1979) 20 25760963
2017 Absence of NUCKS augments paracrine effects of mesenchymal stem cells-mediated cardiac protection. Experimental cell research 19 28412246
2014 Effect of NUCKS-1 overexpression on cytokine profiling in obese women with breast cancer. Asian Pacific journal of cancer prevention : APJCP 19 24568505
2023 NUCKS1, a LINC00629-upregulated gene, facilitated osteosarcoma progression and metastasis by elevating asparagine synthesis. Cell death & disease 17 37528150
2020 MiR-142-3p targeting NUCKS1 inhibits proliferation and invasion of pancreatic cancer cells. Artificial cells, nanomedicine, and biotechnology 16 31914803
2022 LncRNA CBR3-AS1 promotes osteosarcoma progression through the network of miR-140-5p/DDX54-NUCKS1-mTOR signaling pathway. Molecular therapy oncolytics 15 35592388
2019 A microRNA profile of pediatric glioblastoma: The role of NUCKS1 upregulation. Molecular and clinical oncology 15 30847170
2022 NUCKS1 is a highly modified, chromatin-associated protein involved in a diverse set of biological and pathophysiological processes. The Biochemical journal 14 35695515
2014 The comparison of nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) with Ki67 proliferation marker expression in common skin tumors. Polish journal of pathology : official journal of the Polish Society of Pathologists 13 25119009
2013 Dynamic expression of the vertebrate-specific protein Nucks during rodent embryonic development. Gene expression patterns : GEP 13 24140890
2024 NUCB2 inhibition antagonizes osteosarcoma progression and promotes anti-tumor immunity through inactivating NUCKS1/CXCL8 axis. Cancer letters 12 38636892
2018 Nondysplastic Ulcerative Colitis Has High Levels of the Homologous Recombination Repair Protein NUCKS1 and Low Levels of the DNA Damage Marker Gamma-H2AX. Inflammatory bowel diseases 12 29462394
2017 Genetic Polymorphism of NUCKS1 Is Associated With the Susceptibility of Adolescent Idiopathic Scoliosis. Spine 12 28338576
2023 NUCKS1 promotes the progression of colorectal cancer via activating PI3K/AKT/mTOR signaling pathway. Neoplasma 10 37226932
2015 Hypothalamic NUCKS regulates peripheral glucose homoeostasis. The Biochemical journal 9 26205492
2016 Nucks1 synergizes with Trp53 to promote radiation lymphomagenesis in mice. Oncotarget 8 27542204
2016 Identification of kinases phosphorylating 13 sites in the nuclear, DNA-binding protein NUCKS. Biochimica et biophysica acta. Proteins and proteomics 8 28011258
2021 CircRNA circ_0008037 facilitates tumor growth and the Warburg effect via upregulating NUCKS1 by binding to miR-433-3p in non-small cell lung cancer. Thoracic cancer 7 34850570
2019 Functional association between NUCKS1 gene and Parkinson disease: A potential susceptibility biomarker. Bioinformation 7 31719764
2021 NUCKS Promotes the Proliferation, Migration and Invasion of Lung Cancer Cells Through Pi3k/Akt Signalling Pathway. Clinical and investigative medicine. Medecine clinique et experimentale 4 34152708
2023 Roles of increased NUCKS1 expression in endometriosis. BMC women's health 3 37582772
2021 Ese-3 Inhibits the Proliferation, Migration, and Invasion of HaCaT Cells by Downregulating PSIP1 and NUCKS1. Annals of clinical and laboratory science 3 34452885
2021 Nucks1 gene polymorphism rs823114 is associated with the positive symptoms and neurocognitive function of patients with schizophrenia in parts of southern China. Psychiatric genetics 2 34030174
2025 NUCKS1 promotes invasion and metastasis of colorectal cancer by stabilizing HDAC2 and activating AKT. Oncogenesis 1 40527876
2025 BDNF improves fracture healing through promoting osteoblasts proliferation and migration via trkb/akt regulated NUCKS1 expression. Scientific reports 1 40715274
2024 Silencing lncRNA GABPB1-AS1 alleviates cerebral ischemia reperfusion injury through the miR-641/NUCKS1 axis. American journal of translational research 1 39114718
2026 Ubiquitination of DDX21 by HERC2 induces a dormancy-like phenotype via the NUCKS1-p21/p27 axis to promote radio-resistance in colorectal cancer cells. Cell death & disease 0 42071007
2025 NUCKS1 exacerbates hepatocellular carcinoma cell proliferation and metastasis via the upregulation of Cdc42. American journal of cancer research 0 40226459
2025 NUCKS1, a gene targeted by miR-497-5p, suppresses the antitumor effect of Brusatol and enhances malignancy of HNSCC via upregulating S100A9 expression. Biochemical pharmacology 0 40946978
2024 Targeting NUCKS1 with a fragment of tRNAAsn(GUU) of Chinese yew for the treatment of colorectal cancer. Non-coding RNA research 0 39736854

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