Affinage

WDR48

WD repeat-containing protein 48 · UniProt Q8TAF3

Length
677 aa
Mass
76.2 kDa
Annotated
2026-06-11
100 papers in source corpus 18 papers cited in narrative 18 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

WDR48 (UAF1) is a WD40-repeat protein that functions as a shared allosteric activator subunit for a subfamily of deubiquitinating enzymes—USP1, USP12, and USP46—stimulating their catalytic turnover (kcat) without altering substrate affinity (KM) (PMID:18082604, PMID:19075014). Structural studies define the mechanism: UAF1 docks onto the distal end of the substrate USP Fingers domain via a high-affinity site, triggering a cascade of conformational changes that reach a ubiquitin-contacting loop near the catalytic cleft (PMID:27373336, PMID:27650958). In the USP12/USP46 complexes a second WD40 protein, WDR20, joins to form a ternary assembly that further stimulates activity, whereas USP1/UAF1 lacks WDR20 (PMID:20147737). The USP1/UAF1 complex is the central effector in DNA repair: it is imported to the nucleus via USP1 nuclear localization signals, its activity is gated by CDK1 phosphorylation of USP1-Ser313, and it deubiquitinates the Fanconi anemia and replication substrates FANCD2-Ub and PCNA-Ub to support homologous recombination repair (PMID:23116119, PMID:24001775, PMID:22701671). Efficient FANCD2 deubiquitination depends on UAF1 DNA binding and an extensive UAF1–FANCI interface that remodels the FANCI-FANCD2 substrate (PMID:31253762, PMID:33795880). UAF1 also acts through SUMO-like domains to recruit RAD51AP1, enhancing RAD51-mediated DNA pairing and promoting a later step of HR—a function that can proceed independently of USP1 (PMID:27239033, PMID:27463890). Beyond genome maintenance, the USP12/UAF1 complex deubiquitinates and stabilizes the tumor suppressor PHLPP1 to suppress PI3K/Akt signaling, and UAF1-containing complexes remove K48-linked ubiquitin from immune regulators TBK1 and NLRP3 to control interferon and inflammasome responses (PMID:24145035, PMID:29138248, PMID:33247121). WDR48 is also targeted by HPV E1 helicase, which relocalizes it to the nucleus to maintain viral episomes (PMID:18032488).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2007 High

    Established that WDR48/UAF1 is not a passive scaffold but a catalytic activator, answering how USP1 achieves physiological deubiquitinase activity.

    Evidence Affinity purification from HeLa cells with in vitro reconstitution and kinetic analysis using Ub-AMC and monoubiquitinated FANCD2

    PMID:18082604

    Open questions at the time
    • Structural basis of activation unresolved at this stage
    • Did not address whether other USPs share this regulation
  2. 2007 Medium

    Identified WDR48 as a host factor hijacked by HPV E1 helicase, linking it to viral genome persistence.

    Evidence TAP-MS, co-IP, colocalization, and HPV episome maintenance assay in keratinocytes

    PMID:18032488

    Open questions at the time
    • Mechanism by which WDR48 supports episome maintenance not defined
    • Single lab; whether DUB activity is required is unaddressed
  3. 2008 High

    Generalized UAF1's role by showing it is a shared activating subunit of three distinct DUB complexes (USP1, USP12, USP46), defining a regulated DUB subfamily.

    Evidence Reciprocal co-IP, complex purification, and in vitro DUB activity assays across three complexes

    PMID:19075014

    Open questions at the time
    • Substrates of USP12/UAF1 and USP46/UAF1 not yet identified
    • Did not explain selectivity of partner usage
  4. 2010 High

    Resolved complex-specific composition by showing WDR20 selectively joins USP12/USP46—but not USP1—complexes and provides additional activation.

    Evidence Affinity purification, co-IP, siRNA, and in vitro DUB assays with and without WDR20

    PMID:20147737

    Open questions at the time
    • Structural basis of WDR20 contribution not determined here
    • Functional consequence of WDR20 absence in USP1 complex unclear
  5. 2011 High

    Placed the USP1/UAF1 complex functionally within DNA double-strand break repair by showing it promotes HR partly by suppressing NHEJ.

    Evidence Gene knockout in chicken DT40 cells, chemical sensitivity assays, and epistasis with NHEJ-deficient mutants

    PMID:21482670

    Open questions at the time
    • Molecular mechanism linking DUB activity to NHEJ suppression not defined
    • Relevant substrate driving the HR phenotype not pinpointed
  6. 2012 High

    Defined how the complex is spatially and temporally controlled: USP1 NLSs import UAF1 into the nucleus, and CDK1 phosphorylation of USP1-Ser313 gates complex formation and activity.

    Evidence Cellular relocation assays, deletion mutagenesis, immunofluorescence, kinase/phosphatase and DUB activity assays

    PMID:22701671 PMID:23116119

    Open questions at the time
    • Cell-cycle dynamics of activation in vivo not fully mapped
    • Phosphatase responsible for inactivation in cells not identified
  7. 2013 High

    Demonstrated UAF1 is essential in vivo and confirmed FANCD2-Ub and PCNA-Ub as physiological substrates underlying genome stability.

    Evidence Uaf1 knockout mice, MEF characterization, comet and HR reporter assays, flow cytometry for ubiquitinated DNA repair markers

    PMID:24001775

    Open questions at the time
    • Cause of E7.5 embryonic lethality not mechanistically dissected
    • Did not separate USP1-dependent from USP1-independent contributions
  8. 2013 High

    Extended UAF1 function beyond DNA repair by showing the USP12/UAF1 complex stabilizes PHLPP1 to suppress Akt signaling, with a cancer mutation defective in this role.

    Evidence TAP-MS, co-IP, ubiquitination, viability/apoptosis assays, and L580F mutagenesis

    PMID:24145035

    Open questions at the time
    • WDR20 contribution to PHLPP1 regulation not addressed
    • Tumor relevance of L580F beyond cell models untested
  9. 2013 High

    Confirmed evolutionary conservation and a neuronal role by showing C. elegans WDR-48 activates USP-46 to control GLR-1 glutamate receptor abundance and behavior.

    Evidence In vitro DUB assays plus in vivo genetic loss-of-function and receptor abundance microscopy in worms

    PMID:24356955

    Open questions at the time
    • Mammalian neuronal relevance not established here
    • Direct deubiquitination of the receptor not shown biochemically
  10. 2016 High

    Solved the structural basis of allosteric activation, showing UAF1 binds the Fingers domain and transmits conformational changes to the catalytic cleft via a high-affinity site.

    Evidence X-ray crystallography of USP12–UAF1, ITC, mutagenesis, and DUB activity assays

    PMID:27373336 PMID:27650958

    Open questions at the time
    • Function of the second low-affinity UAF1 site undefined
    • Structures of full-length substrate-engaged complexes still lacking at this point
  11. 2016 High

    Uncovered a USP1-independent UAF1 function in HR through DNA binding and SUMO-like-domain-mediated recruitment of RAD51AP1 to enhance RAD51 strand pairing.

    Evidence Biochemical reconstitution, co-IP, DNA binding assays, RAD51 strand-exchange assays, and cellular RAD51 focus/chromosomal aberration analysis

    PMID:27239033 PMID:27463890

    Open questions at the time
    • Interplay between USP1-dependent and RAD51AP1-dependent HR roles not fully resolved
    • Regulation of UAF1 DNA binding in cells unknown
  12. 2017 Medium

    Expanded the substrate repertoire to innate immunity, showing USP1-UAF1 stabilizes TBK1 by removing K48 ubiquitin to promote interferon responses.

    Evidence Co-IP, ubiquitination assays, siRNA, IFN-β reporter assays, and ML323 pharmacological inhibition

    PMID:29138248

    Open questions at the time
    • No in vitro reconstitution of TBK1 deubiquitination
    • Single lab; in vivo immune phenotype not tested here
  13. 2019 High

    Mechanistically explained why UAF1 DNA binding matters, showing it is required for efficient FANCD2 deubiquitination and that RAD51AP1 can substitute for this function in a trimeric complex.

    Evidence Fully reconstituted biochemical system with DNA binding and deubiquitination assays plus cellular validation

    PMID:31253762

    Open questions at the time
    • How DNA binding positions the substrate not visualized at this stage
    • Physiological switch between UAF1- and RAD51AP1-dependent modes unclear
  14. 2020 Medium

    Defined opposing UAF1 complex roles in inflammasome signaling: USP1/UAF1 stabilizes NLRP3 while USP12/46 complexes stabilize p65 to drive transcription.

    Evidence Co-IP, ubiquitination assays, siRNA, Uaf1-deficient mice, and NLRP3 inflammasome activation assays

    PMID:33247121

    Open questions at the time
    • No in vitro reconstitution of NLRP3 or p65 deubiquitination
    • Single lab; substrate specificity across complexes not biochemically separated
  15. 2021 High

    Provided the substrate-engaged structure, revealing that an extensive UAF1–FANCI interface, not direct catalysis by UAF1, drives FANCD2 deubiquitination through substrate remodeling.

    Evidence X-ray crystallography of USP1-UAF1 and cryo-EM of the complex bound to monoubiquitinated FANCI-FANCD2, with mutagenesis and biochemical assays

    PMID:33795880

    Open questions at the time
    • Structural basis of the RAD51AP1-substituted pathway not resolved
    • How CDK1 phosphorylation alters this assembly structurally unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How UAF1 complex composition and substrate selection are coordinated across its diverse roles—DNA repair, Akt suppression, innate immunity, and viral episome maintenance—within a single cell remains unresolved.
  • No unifying model for context-specific substrate routing among USP1/12/46 complexes
  • Immune substrate deubiquitination lacks in vitro reconstitution
  • Regulation balancing USP1-dependent vs USP1-independent HR functions undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 5 GO:0060090 molecular adaptor activity 4 GO:0003677 DNA binding 2
Localization
GO:0005634 nucleus 2 GO:0005829 cytosol 2
Pathway
R-HSA-73894 DNA Repair 3 R-HSA-168256 Immune System 2 R-HSA-392499 Metabolism of proteins 2 R-HSA-162582 Signal Transduction 1
Partners
FANCIHPV E1PHLPP1RAD51AP1USP1USP12USP46WDR20
Complex memberships
USP1/UAF1 complexUSP12-UAF1-WDR20 ternary complexUSP12/UAF1 complexUSP46/UAF1 complex

Evidence

Reading pass · 18 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2007 WDR48 (UAF1) is a stoichiometric subunit of the USP1 deubiquitinating enzyme complex; UAF1 activates USP1 by increasing its catalytic turnover (kcat) without altering substrate affinity (KM), as demonstrated by in vitro reconstitution with Ub-AMC and purified monoubiquitinated FANCD2 as substrates. Affinity purification from HeLa cells, in vitro reconstitution of DUB activity, kinetic analysis (kcat and KM measurements) Molecular cell High 18082604
2008 UAF1 (WDR48) is a shared activating subunit of at least three distinct deubiquitinating enzyme complexes—USP1/UAF1, USP12/UAF1, and USP46/UAF1—functioning as a bona fide regulatory subunit that stimulates enzymatic activity in each complex. Immunoprecipitation, complex purification, in vitro DUB activity assays The Journal of biological chemistry High 19075014
2010 WDR20, a second WD40-repeat protein, associates exclusively with the USP12/UAF1 and USP46/UAF1 complexes (not USP1/UAF1) to form a ternary complex; WDR20 further stimulates USP12 enzymatic activity beyond that provided by UAF1 alone. Affinity purification, co-immunoprecipitation, in vitro DUB activity assays, siRNA knockdown The Journal of biological chemistry High 20147737
2011 The USP1/UAF1 complex promotes homologous recombination (HR)-mediated double-strand break repair; UAF1-deficient cells are hypersensitive to camptothecin and PARP inhibitors, and disruption of NHEJ in UAF1-deficient cells restores resistance, indicating UAF1 promotes HR at least partly by suppressing NHEJ. Gene knockout in chicken DT40 cells, chemical sensitivity assays, epistasis with NHEJ-deficient mutants Molecular and cellular biology High 21482670
2013 WDR48 (UAF1) in complex with USP12 deubiquitinates PHLPP1, stabilizing it and thereby suppressing PI3K/Akt signaling and promoting apoptosis; a cancer-associated WDR48 somatic mutation (L580F) is defective in stabilizing PHLPP1. Tandem affinity purification, co-immunoprecipitation, ubiquitination assays, cell viability and apoptosis assays, mutagenesis The Journal of biological chemistry High 24145035
2013 C. elegans WDR-48 (orthologue of WDR48/UAF1) binds and stimulates USP-46 catalytic activity in vitro; in vivo, WDR-48 and WDR-20 overexpression increases GLR-1 glutamate receptor abundance in the ventral nerve cord by reducing its ubiquitination, and loss-of-function wdr-48 mutants show decreased GLR-1 and altered locomotion. In vitro DUB activity assays, in vivo receptor abundance measurements (fluorescence microscopy), genetic loss-of-function mutants, ubiquitin conjugate analysis The Journal of biological chemistry High 24356955
2016 Crystal structures of the USP12–UAF1 complex reveal that UAF1 binds the distal end of the USP12 Fingers domain and allosterically activates the enzyme via a cascade of structural changes reaching a ubiquitin-contacting loop near the catalytic cleft, without increasing substrate affinity. X-ray crystallography, biochemical activity assays, mutagenesis Molecular cell High 27373336
2016 UAF1 (WDR48) binds DNA and forms a dimeric complex with RAD51AP1 via SUMO-like domains in UAF1 engaging a SUMO-interacting motif in RAD51AP1; UAF1 enhances RAD51-mediated homologous DNA pairing through this complex in a manner independent of USP1. Biochemical reconstitution, co-immunoprecipitation, DNA binding assays, RAD51-mediated strand exchange assays Cell reports High 27239033
2019 Efficient FANCD2 deubiquitination by the USP1-UAF1 complex requires DNA binding by UAF1; RAD51AP1, whose DNA binding activity can substitute for that of UAF1, forms a UAF1-RAD51AP1-USP1 trimeric complex that enables DNA-dependent FANCD2 deubiquitination. Reconstituted biochemical system, DNA binding assays, deubiquitination assays, cell-based FANCD2 deubiquitination experiments Nature communications High 31253762
2021 Crystal structures of USP1-UAF1 and cryo-EM reconstruction of USP1-UAF1 bound to monoubiquitinated FANCI-FANCD2 reveal that an extensive UAF1–FANCI interface (not direct UAF1 catalytic involvement) is required for FANCD2 deubiquitination, and USP1-UAF1 induces conformational changes in the FANCI-FANCD2 substrate. X-ray crystallography, cryo-EM, mutagenesis, biochemical deubiquitination assays Nature structural & molecular biology High 33795880
2012 Serine 313 phosphorylation of USP1 by CDK1 is required for USP1 interaction with UAF1; an inserted domain (amino acids 235–408) in USP1 mediates the UAF1 interaction in a pSer313-dependent manner; protein phosphatase treatment of USP1 inactivates the USP1/UAF1 complex. Mutagenesis, phosphomimetic substitution, co-immunoprecipitation, in vitro kinase/phosphatase assays, DUB activity assays Biochemistry High 23116119
2013 Homozygous Uaf1 knockout mice die at embryonic day 7.5; Uaf1+/- cells show increased FANCD2-Ub and PCNA-Ub levels, chromosome instability, genotoxin hypersensitivity, and defective homologous recombination repair, establishing UAF1 as essential for embryonic development and HR-mediated DNA repair in vivo. Mouse knockout, MEF characterization, comet assay, HR reporter assays, flow cytometry for DNA damage markers Molecular and cellular biology High 24001775
2017 USP1-UAF1 complex stabilizes TBK1 by removing its K48-linked polyubiquitination, preventing proteasomal degradation; this enhances IRF3 activation and IFN-β secretion during viral infection, and the USP1-UAF1 inhibitor ML323 attenuates IFN-β expression and enhances viral replication. Co-immunoprecipitation, ubiquitination assays, siRNA knockdown, IFN-β reporter assays, pharmacological inhibition The Journal of experimental medicine Medium 29138248
2020 The UAF1/USP1 complex selectively removes K48-linked polyubiquitination from NLRP3 to suppress its degradation; UAF1/USP12 and UAF1/USP46 complexes remove K48-linked polyubiquitination from p65 NF-κB to promote NLRP3 and pro-IL-1β transcription; Uaf1 deficiency in vivo attenuates NLRP3 inflammasome activation and IL-1β secretion. Co-immunoprecipitation, ubiquitination assays, siRNA knockdown, Uaf1-deficient mice, NLRP3 inflammasome activation assays Nature communications Medium 33247121
2007 WDR48 (p80) interacts with the N-terminal 40 amino acids of HPV E1 helicase; this interaction is conserved among anogenital HPV types; E1 redistributes WDR48 from the cytoplasm to the nucleus via E1's nuclear localization signal; mutations in E1 that abrogate p80 binding prevent maintenance of HPV episomes in keratinocytes. Tandem affinity purification/mass spectrometry, co-immunoprecipitation, colocalization microscopy, mutagenesis, HPV episome maintenance assay in keratinocytes Journal of virology Medium 18032488
2012 USP1 nuclear localization signals (NLSs) mediate nuclear import of the USP1/UAF1 complex; UAF1 alone localizes to the cytoplasm but is carried into the nucleus by USP1; a conserved 100 amino acid motif in USP1 mediates the UAF1-binding interaction. Cellular relocation assay, deletion/mutagenesis analysis, immunofluorescence microscopy PloS one Medium 22701671
2016 Crystal structure of USP12-Ub/UAF1 reveals two UAF1 binding sites on USP12 (stoichiometry 1:2 in solution); the high-affinity site (Kd ~4 nM) at the Fingers subdomain is required for activation, while the second lower-affinity site (Kd ~325 nM) does not affect activation; this two-step binding is conserved in USP1. X-ray crystallography, isothermal titration calorimetry, mutagenesis, in vitro DUB activity assays Journal of structural biology High 27650958
2016 UAF1 mediates interaction between USP1 and RAD51AP1; depletion of USP1 or UAF1 decreases RAD51AP1 stability; cells expressing a UAF1-interaction-deficient RAD51AP1 mutant show increased chromosomal aberrations and persistent RAD51 foci after DNA damage, indicating the USP1-UAF1 complex promotes a later step of HR repair through RAD51AP1. Co-immunoprecipitation, protein interaction mapping, siRNA knockdown, chromosomal aberration assay, RAD51 focus analysis Cell cycle Medium 27463890

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 ALK1 and p80 expression and chromosomal rearrangements involving 2p23 in inflammatory myofibroblastic tumor. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 443 11406658
1991 Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin. The Journal of experimental medicine 329 2033369
2007 A UAF1-containing multisubunit protein complex regulates the Fanconi anemia pathway. Molecular cell 306 18082604
2002 Expression of ALK1 and p80 in inflammatory myofibroblastic tumor and its mesenchymal mimics: a study of 135 cases. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 268 12218210
1996 Characterization of the transforming activity of p80, a hyperphosphorylated protein in a Ki-1 lymphoma cell line with chromosomal translocation t(2;5). Proceedings of the National Academy of Sciences of the United States of America 249 8633037
1990 Antibodies against the CD44 p80, lymphocyte homing receptor molecule augment human peripheral blood T cell activation. Journal of immunology (Baltimore, Md. : 1950) 238 1967267
2014 A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nature chemical biology 227 24531842
1989 Monoclonal antibodies against the CD44 [In(Lu)-related p80], and Pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors. Journal of immunology (Baltimore, Md. : 1950) 196 2646376
2011 Selective and cell-active inhibitors of the USP1/ UAF1 deubiquitinase complex reverse cisplatin resistance in non-small cell lung cancer cells. Chemistry & biology 194 22118673
1996 High incidence of the t(2;5)(p23;q35) translocation in anaplastic large cell lymphoma and its lack of detection in Hodgkin's disease. Comparison of cytogenetic analysis, reverse transcriptase-polymerase chain reaction, and P-80 immunostaining. Blood 193 8547653
1997 Differential regulation of the p80 tumor necrosis factor receptor in human obesity and insulin resistance. Diabetes 187 9032102
1997 Enhancement of TNF receptor p60-mediated cytotoxicity by TNF receptor p80: requirement of the TNF receptor-associated factor-2 binding site. Journal of immunology (Baltimore, Md. : 1950) 163 9036990
2008 UAF1 is a subunit of multiple deubiquitinating enzyme complexes. The Journal of biological chemistry 159 19075014
1994 TNF induces internalization of the p60 receptor and shedding of the p80 receptor. Journal of immunology (Baltimore, Md. : 1950) 156 8144934
1995 Pestivirus NS3 (p80) protein possesses RNA helicase activity. Journal of virology 151 7853509
1996 Recombinant soluble tumor necrosis factor receptor (p80) fusion protein: toxicity and dose finding trial in refractory rheumatoid arthritis. The Journal of rheumatology 143 8923355
1998 Overexpression of the p80 TNF receptor leads to TNF-dependent apoptosis, nuclear factor-kappa B activation, and c-Jun kinase activation. Journal of immunology (Baltimore, Md. : 1950) 139 9531270
1995 Mutational analysis of p80 coilin indicates a functional interaction between coiled bodies and the nucleolus. The Journal of cell biology 137 7490287
1990 Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome. Proceedings of the National Academy of Sciences of the United States of America 137 2308937
2000 Two domains of p80 katanin regulate microtubule severing and spindle pole targeting by p60 katanin. Journal of cell science 127 10751153
2000 A crucial role for p80 TNF-R2 in amplifying p60 TNF-R1 apoptosis signals in T lymphocytes. European journal of immunology 125 10671223
1983 Differentiation of human T lymphocytes. I. Acquisition of a novel human cell surface protein (p80) during normal intrathymic T cell maturation. Journal of immunology (Baltimore, Md. : 1950) 120 6224850
1997 Anaplastic large cell lymphoma: a distinct molecular pathologic entity: a reappraisal with special reference to p80(NPM/ALK) expression. The American journal of surgical pathology 118 9414185
2020 UAF1 deubiquitinase complexes facilitate NLRP3 inflammasome activation by promoting NLRP3 expression. Nature communications 112 33247121
2011 The USP1/UAF1 complex promotes double-strand break repair through homologous recombination. Molecular and cellular biology 107 21482670
1995 p80/85 cortactin associates with the Src SH2 domain and colocalizes with v-Src in transformed cells. The Journal of biological chemistry 106 7592885
1993 RNA-stimulated NTPase activity associated with the p80 protein of the pestivirus bovine viral diarrhea virus. Virology 103 8382392
1986 Identification of Ki (Ku, p70/p80) autoantigens and analysis of anti-Ki autoantibody reactivity. Journal of immunology (Baltimore, Md. : 1950) 102 2419410
1999 Expression and localization of p80 and p68 interleukin-1 receptor proteins in the brain of adult mice. Journal of neuroimmunology 88 10378883
2014 Katanin p80 regulates human cortical development by limiting centriole and cilia number. Neuron 86 25521379
1994 Chromosomal location and expression of the genes coding for Ku p70 and p80 in human cell lines and normal tissues. Cytogenetics and cell genetics 86 8258294
2004 Adhesion or plasmin regulates tyrosine phosphorylation of a novel membrane glycoprotein p80/gp140/CUB domain-containing protein 1 in epithelia. The Journal of biological chemistry 84 14739293
2012 An essential role for katanin p80 and microtubule severing in male gamete production. PLoS genetics 81 22654669
2010 WDR20 regulates activity of the USP12 x UAF1 deubiquitinating enzyme complex. The Journal of biological chemistry 81 20147737
2004 The effects of ketamine vary among inbred mouse strains and mimic schizophrenia for the P80, but not P20 or N40 auditory ERP components. Neurochemical research 79 15176475
1992 Antibodies to the p70/p80 (Ku) antigens in systemic lupus erythematosus. Rheumatic diseases clinics of North America 74 1626075
2017 USP1-UAF1 deubiquitinase complex stabilizes TBK1 and enhances antiviral responses. The Journal of experimental medicine 69 29138248
2007 Differential roles of p80- and p130-angiomotin in the switch between migration and stabilization of endothelial cells. Biochimica et biophysica acta 69 18164266
1998 Identification of two domains of the p70 Ku protein mediating dimerization with p80 and DNA binding. The Journal of biological chemistry 67 9422740
1993 Analysis of p60 and p80 tumor necrosis factor-alpha receptor messenger RNA and protein in human placentas. The American journal of pathology 64 8214007
2013 WD repeat protein WDR48 in complex with deubiquitinase USP12 suppresses Akt-dependent cell survival signaling by stabilizing PH domain leucine-rich repeat protein phosphatase 1 (PHLPP1). The Journal of biological chemistry 62 24145035
1989 CD44 antibody against In(Lu)-related p80, lymphocyte-homing receptor molecule inhibits the binding of human erythrocytes to T cells. Journal of immunology (Baltimore, Md. : 1950) 62 2480381
2011 Preclinical prophylactic efficacy testing of Sm-p80-based vaccine in a nonhuman primate model of Schistosoma mansoni infection and immunoglobulin G and E responses to Sm-p80 in human serum samples from an area where schistosomiasis is endemic. The Journal of infectious diseases 61 21921206
2004 PF15p is the chlamydomonas homologue of the Katanin p80 subunit and is required for assembly of flagellar central microtubules. Eukaryotic cell 61 15302820
2016 Allosteric Activation of Ubiquitin-Specific Proteases by β-Propeller Proteins UAF1 and WDR20. Molecular cell 60 27373336
2016 Food Additive P-80 Impacts Mouse Gut Microbiota Promoting Intestinal Inflammation, Obesity and Liver Dysfunction. SOJ microbiology & infectious diseases 58 27430014
2003 Enhancement of Sm-p80 (large subunit of calpain) induced protective immunity against Schistosoma mansoni through co-delivery of interleukin-2 and interleukin-12 in a DNA vaccine formulation. Vaccine 58 12798631
2014 Synthesis and structure-activity relationship studies of N-benzyl-2-phenylpyrimidin-4-amine derivatives as potent USP1/UAF1 deubiquitinase inhibitors with anticancer activity against nonsmall cell lung cancer. Journal of medicinal chemistry 57 25229643
1994 Intratracheal administration of endotoxin and cytokines. VII. The soluble interleukin-1 receptor and the soluble tumor necrosis factor receptor II (p80) inhibit acute inflammation. Clinical immunology and immunopathology 56 8020186
2019 DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nature communications 55 31253762
1998 Interaction of recombinant Tetrahymena telomerase proteins p80 and p95 with telomerase RNA and telomeric DNA substrates. Genes & development 55 9499406
2009 Prime-boost and recombinant protein vaccination strategies using Sm-p80 protects against Schistosoma mansoni infection in the mouse model to levels previously attainable only by the irradiated cercarial vaccine. Parasitology research 54 19809833
1994 Human p80-coilin is targeted to sphere organelles in the amphibian germinal vesicle. Molecular biology of the cell 54 7532471
1990 Antigenic determinants of the Ku (p70/p80) autoantigen are poorly conserved between species. Journal of immunology (Baltimore, Md. : 1950) 54 1701785
2003 Induction of protective immunity against Schistosoma mansoni via DNA priming and boosting with the large subunit of calpain (Sm-p80): adjuvant effects of granulocyte-macrophage colony-stimulating factor and interleukin-4. Infection and immunity 53 12819068
1991 Epitopes of the p70 and p80 (Ku) lupus autoantigens. Journal of immunology (Baltimore, Md. : 1950) 53 1707916
1990 In vivo modulation with anti-interleukin-1 (IL-1) receptor (p80) antibody 35F5 of the response to IL-1. The relationship of radioprotection, colony-stimulating factor, and IL-6. Blood 53 2142008
2009 Protective and antifecundity effects of Sm-p80-based DNA vaccine formulation against Schistosoma mansoni in a nonhuman primate model. Vaccine 52 19366570
2018 Sm-p80-based schistosomiasis vaccine: double-blind preclinical trial in baboons demonstrates comprehensive prophylactic and parasite transmission-blocking efficacy. Annals of the New York Academy of Sciences 51 30133707
2016 Promotion of RAD51-Mediated Homologous DNA Pairing by the RAD51AP1-UAF1 Complex. Cell reports 51 27239033
2001 Genetic deletion of the tumor necrosis factor receptor p60 or p80 abrogates ligand-mediated activation of nuclear factor-kappa B and of mitogen-activated protein kinases in macrophages. The Journal of biological chemistry 50 11438547
1992 Heterogeneous expression of the non-structural protein p80/p125 in cells infected with different pestiviruses. The Journal of general virology 48 1309861
1992 Immunolocalization of Ku-proteins (p80/p70): localization of p70 to nucleoli and periphery of both interphase nuclei and metaphase chromosomes. Experimental cell research 48 1639139
1994 Structure, expression and chromosomal localization of human p80-coilin gene. Nucleic acids research 47 7971277
2021 Structural basis of FANCD2 deubiquitination by USP1-UAF1. Nature structural & molecular biology 45 33795880
1992 Inhibition of ligand binding and antiproliferative effects of tumor necrosis factor and lymphotoxin by soluble forms of recombinant P60 and P80 receptors. Biochemical and biophysical research communications 44 1310388
2016 Proteomic Analysis of the Mammalian Katanin Family of Microtubule-severing Enzymes Defines Katanin p80 subunit B-like 1 (KATNBL1) as a Regulator of Mammalian Katanin Microtubule-severing. Molecular & cellular proteomics : MCP 42 26929214
2014 Use of an Sm-p80-based therapeutic vaccine to kill established adult schistosome parasites in chronically infected baboons. The Journal of infectious diseases 42 24436452
2009 Sm-p80-based DNA vaccine formulation induces potent protective immunity against Schistosoma mansoni. Parasite immunology 42 19222788
2002 p80 ROKalpha binding protein is a novel splice variant of CRMP-1 which associates with CRMP-2 and modulates RhoA-induced neuronal morphology. FEBS letters 42 12482610
2010 Sm-p80-based DNA vaccine provides baboons with levels of protection against Schistosoma mansoni infection comparable to those achieved by the irradiated cercarial vaccine. The Journal of infectious diseases 41 20187746
1994 Assembly and DNA binding of recombinant Ku (p70/p80) autoantigen defined by a novel monoclonal antibody specific for p70/p80 heterodimers. Journal of cell science 41 7699019
2013 The WD40-repeat proteins WDR-20 and WDR-48 bind and activate the deubiquitinating enzyme USP-46 to promote the abundance of the glutamate receptor GLR-1 in the ventral nerve cord of Caenorhabditis elegans. The Journal of biological chemistry 39 24356955
2007 LAPSER1 is a putative cytokinetic tumor suppressor that shows the same centrosome and midbody subcellular localization pattern as p80 katanin. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39 17351128
2007 Human papillomavirus E1 helicase interacts with the WD repeat protein p80 to promote maintenance of the viral genome in keratinocytes. Journal of virology 38 18032488
1995 p80-coilin: a component of coiled bodies and interchromatin granule-associated zones. Journal of cell science 38 7622600
1992 Nucleotide sequence and transcriptional analysis of the p80 gene of Autographa californica nuclear polyhedrosis virus: a homologue of the Orgyia pseudotsugata nuclear polyhedrosis virus capsid-associated gene. Virology 38 1529529
1996 Differential sensitivity to Escherichia coli infection in mice lacking tumor necrosis factor p55 or interleukin-1 p80 receptors. Archives of surgery (Chicago, Ill. : 1960) 37 8911263
1992 Down-modulation of cell surface expression of p80 form of the tumor necrosis factor receptor by human immunodeficiency virus-1 tat gene. Lymphokine and cytokine research 37 1335762
2017 Katanin p80, NuMA and cytoplasmic dynein cooperate to control microtubule dynamics. Scientific reports 35 28079116
2013 Inactivation of Uaf1 causes defective homologous recombination and early embryonic lethality in mice. Molecular and cellular biology 35 24001775
2012 Serine phosphorylation is critical for the activation of ubiquitin-specific protease 1 and its interaction with WD40-repeat protein UAF1. Biochemistry 35 23116119
1997 The t(2;5)-associated p80 NPM/ALK fusion protein in nodal and cutaneous CD30+ lymphoproliferative disorders. Journal of cutaneous pathology 34 9449486
1995 Role of free p70 (Ku) subunit in posttranslational stabilization of newly synthesized p80 during DNA-dependent protein kinase assembly. European journal of cell biology 34 7774599
1994 Detection of bovine viral diarrhoea virus p80 protein in subpopulations of bovine leukocytes. The Journal of general virology 34 8176381
2014 Longevity of Sm-p80-specific antibody responses following vaccination with Sm-p80 vaccine in mice and baboons and transplacental transfer of Sm-p80-specific antibodies in a baboon. Parasitology research 32 24728521
2010 Protective effects of Sm-p80 in the presence of resiquimod as an adjuvant against challenge infection with Schistosoma mansoni in mice. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 32 20630783
2003 Genetic deletion of the tumor necrosis factor receptor p60 or p80 sensitizes macrophages to lipopolysaccharide-induced nuclear factor-kappa B, mitogen-activated protein kinases, and apoptosis. The Journal of biological chemistry 32 12695514
2016 The USP1-UAF1 complex interacts with RAD51AP1 to promote homologous recombination repair. Cell cycle (Georgetown, Tex.) 31 27463890
2011 Role of antibodies in Sm-p80-mediated protection against Schistosoma mansoni challenge infection in murine and nonhuman primate models. Vaccine 31 21277404
2009 Angiomotin p80/p130 ratio: a new indicator of exercise-induced angiogenic activity in skeletal muscles from obese and non-obese rats? The Journal of physiology 31 19546164
2005 The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle. Nucleic acids research 31 15701761
2017 Cross-species prophylactic efficacy of Sm-p80-based vaccine and intracellular localization of Sm-p80/Sm-p80 ortholog proteins during development in Schistosoma mansoni, Schistosoma japonicum, and Schistosoma haematobium. Parasitology research 30 29026995
2012 Two nuclear localization signals in USP1 mediate nuclear import of the USP1/UAF1 complex. PloS one 30 22701671
2018 Sm-p80-based vaccine trial in baboons: efficacy when mimicking natural conditions of chronic disease, praziquantel therapy, immunization, and Schistosoma mansoni re-encounter. Annals of the New York Academy of Sciences 29 29888790
2016 A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme. Journal of structural biology 29 27650958
2012 Role of antibody dependent cell mediated cytotoxicity (ADCC) in Sm-p80-mediated protection against Schistosoma mansoni. Vaccine 29 23000221
2005 Characterization of the immune response to DNA vaccination strategies for schistosomiasis candidate antigen, Sm-p80 in the baboon. Vaccine 29 15670880
1999 The immunohistochemical expression of DNA-PKCS and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity. International journal of radiation oncology, biology, physics 29 10571209
1996 Dual expression of p80 type I and p68 type II interleukin-I receptors on anterior pituitary cells synthesizing growth hormone. Endocrinology 29 8756580

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