Affinage

KPNB1

Importin subunit beta-1 · UniProt Q14974

Length
876 aa
Mass
97.2 kDa
Annotated
2026-06-10
38 papers in source corpus 23 papers cited in narrative 24 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KPNB1 (karyopherin β1/importin β1) is the principal nuclear import receptor that ferries a structurally and functionally diverse set of cargo proteins through the nuclear pore complex, operating both through the classical importin α (KPNA2) adaptor pathway and through direct, importin-α-independent recognition of cargo nuclear localization signals (PMID:23906023, PMID:35635291, PMID:39604846). It drives nuclear translocation of inducible transcription factors—NF-κB/p65 following TNF-α stimulation (PMID:23906023), the PER/CRY repressor complex to close the negative feedback loop of the mammalian circadian clock (PMID:26319354), E2F1 (PMID:31819526), the tonicity-responsive factor NFAT5 via direct binding to a unique NFAT5-NLS (PMID:35635291), and ATF4 via recognition of residues 280-299 of its NLS to license downstream BNIP3-dependent mitophagy (PMID:39604846). The same receptor imports nuclear-localized PD-L1, IRF1, YBX1, VCP, and HMGB2, coupling KPNB1 to immune-evasion programs and DNA damage repair (PMID:33139930, PMID:34069326, PMID:38254206, PMID:40339118, PMID:40082556). As an apical hub for nucleocytoplasmic transport, KPNB1 functions as a master regulator of cell-cycle proteins, and its genetic or pharmacological inhibition triggers cytosolic retention of nuclear cargo, polyubiquitination, aggresome-like structure formation, and chronic unfolded protein response signaling through the eIF2α/ATF4 axis, culminating in Bcl-2-family-mediated apoptosis (PMID:28811376, PMID:29520102). KPNB1 protein abundance is set by competing ubiquitination events—USP7-mediated deubiquitination and TMEM209-mediated shielding from RCHY1-dependent K48-linked degradation stabilize the receptor, while an EZH2-controlled miR-30a/miR-30d axis represses it post-transcriptionally (PMID:24132643, PMID:25890085, PMID:38254206, PMID:39414762). Withaferin A covalently engages Cys158 of KPNB1 to block VCP import and impair DNA damage repair, defining a druggable site on the receptor (PMID:40339118).

Mechanistic history

Synthesis pass · year-by-year structured walk · 23 steps
  1. 2013 High

    Established which importin β family member carries activated NF-κB into the nucleus, defining KPNB1 as the dominant receptor for inflammatory transcription factor import.

    Evidence High-content siRNA screen across 17 importin β members with nuclear fractionation and NF-κB reporter assays in TNF-α-stimulated cells

    PMID:23906023

    Open questions at the time
    • Did not resolve whether p65 import is fully importin-α dependent or partly direct
    • No structural detail of cargo recognition
  2. 2014 High

    Connected KPNB1 abundance to an upstream epigenetic/miRNA circuit, showing KPNB1 is a survival effector downstream of EZH2 via miR-30d derepression.

    Evidence Genetic epistasis with EZH2 knockdown, miR-30d overexpression, KPNB1 rescue, 3'UTR luciferase reporter, and xenografts in MPNST

    PMID:24132643

    Open questions at the time
    • Generalizability beyond MPNST not tested
    • Direct miRNA-mRNA binding inferred from reporter only
  3. 2015 High

    Demonstrated KPNB1 can act independently of importin α, importing the PER/CRY repressor to complete circadian negative feedback.

    Evidence RNAi with fractionation and reciprocal Co-IP in human cells plus inducible importin β inhibition in Drosophila lateral neurons

    PMID:26319354

    Open questions at the time
    • NLS/binding interface on PER not mapped
    • Stoichiometry of PER/CRY complex import unresolved
  4. 2017 High

    Defined KPNB1 as a master regulator of cell-cycle protein localization whose loss causes multiphase arrest and death, validating it as a cancer dependency.

    Evidence Orthogonal shRNA dropout and genome-wide CRISPR/Cas9 screens with proteomics and pharmacological inhibition in ovarian cancer

    PMID:28811376

    Open questions at the time
    • Direct vs indirect effects on p21/p27/APC/C not fully separated
    • Which cargo losses are rate-limiting for arrest unclear
  5. 2017 Medium

    Showed KPNB1 inhibition simultaneously blocks NF-κB and AP-1 import, linking the receptor to cytokine output and invasion via a small-molecule inhibitor.

    Evidence siRNA and INI-43 inhibition with fractionation, reporter assays, and Transwell migration/invasion in cervical cancer

    PMID:28427184

    Open questions at the time
    • Inhibitor selectivity not exhaustively profiled
    • Single lab, single tumor type
  6. 2018 High

    Mechanistically explained how KPNB1 loss kills cells: cytosolic cargo accumulation triggers proteotoxic UPR and Bcl-2-family-driven apoptosis.

    Evidence Genetic and pharmacological inhibition with ubiquitination/UPR Western blots and rescue by KPNB1 overexpression or translation inhibitors in glioblastoma

    PMID:29520102

    Open questions at the time
    • Identity of cargo whose mislocalization initiates UPR not pinpointed
    • Tumor-type specificity of UPR threshold unknown
  7. 2019 Medium

    Extended the death mechanism to TRAIL sensitization and identified an autophagy-mediated resistance counterforce upon KPNB1 inhibition.

    Evidence siRNA and pharmacological inhibition with caspase assays, DISC component blots, and autophagy flux inhibitors in glioblastoma

    PMID:30742128

    Open questions at the time
    • Whether autophagy counteraction generalizes beyond glioblastoma untested
    • Single lab
  8. 2019 Medium

    Identified E2F1 as a KPNB1 cargo, linking import to c-Myc/KPNA2 expression and G2/M control in CML.

    Evidence siRNA and importazole inhibition with E2F1 immunofluorescence, target Western blots, and flow cytometry

    PMID:31819526

    Open questions at the time
    • Direct KPNB1-E2F1 binding not shown
    • Importazole off-target effects not excluded
  9. 2020 Medium

    Revealed a non-canonical nuclear role for PD-L1 trafficked by KPNB1, coupling it to Sp1-driven Gas6/MerTK proliferative signaling.

    Evidence Co-IP, fractionation, PD-L1 knockdown, ChIP on Gas6 promoter, and in vivo proliferation assays in NSCLC

    PMID:33139930

    Open questions at the time
    • NLS on PD-L1 recognized by KPNB1 not mapped
    • Single lab
  10. 2020 Medium

    Confirmed canonical KPNA2/KPNB1 cooperation for an oncogenic cargo (NLS-RARα) controlling myeloid differentiation.

    Evidence IP-MS, Co-IP validation, siRNA, fractionation, and immunofluorescence

    PMID:32696739

    Open questions at the time
    • Relative contribution of α vs β not quantified
    • Single lab
  11. 2021 Medium

    Placed KPNB1 in the radiation-induced immune-evasion axis by importing IRF1 to upregulate surface PD-L1.

    Evidence IRF1 siRNA, importazole inhibition, IRF1 localization imaging, and surface PD-L1 flow cytometry

    PMID:34069326

    Open questions at the time
    • Direct KPNB1-IRF1 binding not demonstrated
    • Importazole specificity caveat
  12. 2022 High

    Provided clean evidence for importin-α-independent cargo recognition via a unique NFAT5-NLS, sharpening KPNB1's direct-binding mode.

    Evidence Importin-family siRNA screen, Co-IP, fractionation, and NLS mutagenesis for tonicity-responsive NFAT5 import

    PMID:35635291

    Open questions at the time
    • Structural basis of the KPNB1-NFAT5-NLS interface not solved
  13. 2022 Medium

    Uncovered an import-independent function: KPNB1 binding to ataxin-3 lowers polyQ aggregate load via CLPP-dependent fragmentation.

    Evidence Co-IP, label-free proteomics, KPNB1 and CLPP perturbation across MJD mouse and iPSC models

    PMID:35794401

    Open questions at the time
    • How KPNB1 routes ataxin-3 to mitochondrial CLPP mechanistically unclear
    • Single lab
  14. 2022 Medium

    Showed adaptor proteins can tune KPNB1 cargo flux, with PCDH1 enhancing p65 import to drive PDAC.

    Evidence PCDH1-KPNB1 Co-IP, p65 fractionation, NF-κB reporter, and proliferation/invasion assays

    PMID:35864095

    Open questions at the time
    • Whether PCDH1 acts as cargo, adaptor, or stabilizer not resolved
    • Mechanism partially indirect
  15. 2023 Medium

    Linked KPNB1-mediated import machinery to nuclear envelope integrity during S-phase through an RTEL1 C-terminal interaction.

    Evidence Co-IP, domain truncation mapping, high-resolution microscopy, and nuclear envelope stability assays

    PMID:38132118

    Open questions at the time
    • Functional consequence of RTEL1-KPNB1 binding for import cargo unknown
    • Single lab
  16. 2024 High

    Established USP7 as a positive regulator of KPNB1 stability and identified YBX1 as a cargo driving NLGN3-dependent glioblastoma progression.

    Evidence Ubiquitination assays, IP-MS, fractionation, immunofluorescence, ChIP on NLGN3 promoter, and intracranial tumor model

    PMID:38254206

    Open questions at the time
    • Site of USP7 deubiquitination on KPNB1 not mapped
    • Direct YBX1-NLS recognition not shown
  17. 2024 Medium

    Defined the degradative arm of KPNB1 stability control: RCHY1 K48-ubiquitination opposed by TMEM209 shielding, feeding Wnt/β-catenin oncogenesis.

    Evidence TMEM209-KPNB1 and KPNB1-RCHY1 Co-IP, ubiquitination assays, Western blot, and xenografts in HCC

    PMID:39414762

    Open questions at the time
    • Direct ubiquitination site on KPNB1 not identified
    • Single lab
  18. 2024 High

    Mapped a precise ATF4 NLS (residues 280-299) recognized by KPNB1, connecting receptor function to BNIP3 mitophagy and odontoblastic differentiation.

    Evidence IP-MS, NLS wild-type/mutant constructs, dual-luciferase and ChIP promoter mapping, and in vivo implantation

    PMID:39604846

    Open questions at the time
    • Structural interface of KPNB1-ATF4-NLS not solved
    • Whether other stress contexts use the same NLS untested
  19. 2024 Low

    Proposed an RNA-modification route (NAT10/ac4C) for upregulating KPNB1 to drive PD-L1 import and radiotherapy resistance.

    Evidence NAT10 knockdown, ac4C assays, KPNB1 overexpression, PD-L1 fractionation, and immune co-culture in NSCLC

    PMID:40109769

    Open questions at the time
    • Direct ac4C deposition on KPNB1 mRNA not biochemically confirmed
    • Effect inferred indirectly from knockdown
    • Single lab
  20. 2025 High

    Identified VCP as a KPNB1 cargo essential for DNA damage repair and defined Cys158 as a covalent druggable site exploited by withaferin A.

    Evidence Chemical pulldown with IP-MS, Co-IP, Cys158 mutagenesis, and in vivo xenograft DDR assays

    PMID:40339118

    Open questions at the time
    • VCP NLS recognized by KPNB1 not mapped
    • Selectivity of Cys158 engagement across karyopherins untested
  21. 2025 Medium

    Extended KPNB1's DDR role to HMGB2 import in AML, providing a venetoclax-sensitization strategy via import blockade.

    Evidence Co-IP, fractionation, siRNA/importazole inhibition, patient-derived viability assays, and xenografts

    PMID:40082556

    Open questions at the time
    • Direct KPNB1-HMGB2 binding interface not defined
    • Single lab
  22. 2025 Medium

    Showed the KPNA2/KPNB1 complex can act protectively by cytoplasmic sequestration of toxic C9orf72 polyPR dipeptides.

    Evidence FITC-PR20 tracking with KPNB1/KPNA2 siRNA, overexpression rescue, and JQ1-mediated downregulation

    PMID:39891383

    Open questions at the time
    • Whether sequestration vs import dominates in neurons untested
    • Single lab
  23. 2025 Medium

    Identified KPNB1 as a reader of O-GlcNAcylated RNA Pol II CTD, linking metabolic modification to polymerase nuclear entry and global transcription.

    Evidence WGA lectin pulldown with MS, structural modeling, wChIP, and siRNA/inhibitor functional assays (preprint)

    Open questions at the time
    • Preprint, not yet peer-reviewed
    • Direct CTD-Ser5-O-GlcNAc binding by KPNB1 needs structural confirmation

Open questions

Synthesis pass · forward-looking unresolved questions
  • The structural rules governing how a single receptor distinguishes importin-α-dependent versus direct-NLS cargo, and how its many described stability inputs are integrated in vivo, remain unresolved.
  • No experimental structure of KPNB1-cargo NLS complexes in the corpus
  • Hierarchy among USP7, RCHY1, TMEM209, and miR-30 control not reconciled
  • Cargo selection determinants for direct vs adaptor-mediated import undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140104 molecular carrier activity 5
Localization
GO:0005634 nucleus 3 GO:0005829 cytosol 3 GO:0005635 nuclear envelope 1
Pathway
R-HSA-9609507 Protein localization 4 R-HSA-1640170 Cell Cycle 2 R-HSA-168256 Immune System 2 R-HSA-73894 DNA Repair 2 R-HSA-8953897 Cellular responses to stimuli 2 R-HSA-9909396 Circadian clock 1
Partners
ATF4KPNA2PCDH1RCHY1RTEL1TMEM209USP7VCP
Complex memberships
KPNA2/KPNB1 importin complex

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2013 KPNB1 is the major importin β receptor mediating NF-κB/p65 nuclear import in a NLS-dependent manner following TNF-α stimulation, acting via the canonical KPNA2/KPNB1 pathway; siRNA knockdown of KPNB1 reduced nuclear p65 and decreased NF-κB transcriptional activity. High-content siRNA screening of 17 importin β family members, followed by nuclear fractionation and reporter assays Traffic (Copenhagen, Denmark) High 23906023
2015 KPNB1 mediates nuclear translocation of PER proteins (and the PER/CRY repressor complex) in a circadian fashion and independently of importin α, thereby enabling negative feedback regulation of the mammalian circadian clock; RNAi depletion of KPNB1 traps PER/CRY in the cytoplasm and disrupts clock function. RNAi knockdown in human cells with cytoplasmic/nuclear fractionation and co-immunoprecipitation; inducible inhibition of Drosophila importin β in lateral neurons abolished behavioral rhythms eLife High 26319354
2014 EZH2 epigenetically suppresses miR-30d transcription, which relieves repression of KPNB1 (miR-30d targets KPNB1 3′ UTR), resulting in elevated KPNB1 that promotes MPNST cell survival; forced KPNB1 overexpression rescues apoptosis induced by EZH2 knockdown, placing KPNB1 downstream of the EZH2-miR-30d axis. Genetic epistasis: EZH2 knockdown, miR-30d overexpression, KPNB1 overexpression rescue; 3′ UTR luciferase reporter assay; xenograft tumor model The Journal of pathology High 24132643
2015 miR-30a also targets the KPNB1 3′ UTR in MPNST cells, contributing to EZH2/miR-30a,d/KPNB1 signaling axis; pharmacological EZH2 inhibition (DZNep) downregulates KPNB1 protein and suppresses tumor growth. 3′ UTR luciferase reporter assay; Western blot; xenograft mouse model with IHC Molecular cancer Medium 25890085
2017 KPNB1 acts as a master regulator of cell cycle-related proteins (including p21, p27, and APC/C); genetic and pharmacological inhibition of KPNB1 causes multiphase cell cycle arrest and apoptosis induction in epithelial ovarian cancer cells in vitro and in vivo. shRNA dropout screen in xenografts, CRISPR/Cas9 genome-wide screen, pharmacological inhibition, proteomics Proceedings of the National Academy of Sciences of the United States of America High 28811376
2017 KPNB1 is required for translocation of NF-κB and AP-1 transcription factors into the nucleus in cervical cancer cells; siRNA or small-molecule inhibition of KPNB1 (INI-43) causes cytoplasmic retention of NF-κB and decreases AP-1 transcriptional activity, reducing expression of IL-6, IL-1β, TNF-α, and GM-CSF, and impairing cancer cell migration and invasion. siRNA knockdown; small-molecule inhibition with INI-43; nuclear fractionation; transcriptional reporter assays; Transwell migration/invasion assays Oncotarget Medium 28427184
2018 KPNB1 inhibition in glioblastoma cells causes cytosolic retention of cargo proteins, elevated polyubiquitination, aggresome-like-induced structure (ALIS) formation, and unfolded protein response (UPR) activation; chronic UPR (eIF2α/ATF4 cascade) upregulates pro-apoptotic Bcl-2 family members Puma and Noxa, frees Bax/Bak from Mcl-1, and induces MOMP and apoptosis. siRNA knockdown; pharmacological inhibition; Western blot for ubiquitination and UPR markers; rescue by KPNB1 overexpression or protein synthesis inhibitors; combination with proteasome/autophagy inhibitors Oncogene High 29520102
2019 KPNB1 inhibition in glioblastoma potentiates TRAIL-induced apoptosis via UPR-mediated ATF4 upregulation of DR5 and 4E-BP1 (suppressing cap-dependent translation of FLIPL/FLIPS), freeing Bax/Bak from Mcl-1, and promoting DISC assembly; KPNB1 inhibition-induced autophagy counteracts by degrading cleaved caspase-8. Genetic (siRNA) and pharmacological KPNB1 inhibition; caspase activity assays; Western blot for DISC components; autophagy flux inhibitors Cell death & disease Medium 30742128
2019 KPNB1 mediates nuclear import of E2F1 in CML cells; KPNB1 inhibition (siRNA or importazole) blocks E2F1 nuclear translocation, reduces c-Myc and KPNA2 expression downstream of E2F1, and induces G2/M arrest and apoptosis. siRNA knockdown; pharmacological inhibition (importazole); immunofluorescence for E2F1 localization; Western blot for E2F1 targets; flow cytometry OncoTargets and therapy Medium 31819526
2020 KPNB1 facilitates nuclear translocation of PD-L1 in NSCLC cells; nuclear PD-L1 (nPD-L1), coupled with transcription factor Sp1, drives Gas6 mRNA synthesis and secretion, activating MerTK signaling to promote cancer cell proliferation. Co-immunoprecipitation (KPNB1-PD-L1 binding); nuclear fractionation; PD-L1 knockdown; ChIP for Sp1/nPD-L1 on Gas6 promoter; in vitro and in vivo proliferation assays Cell death and differentiation Medium 33139930
2021 KPNB1 mediates nuclear import of IRF1 following ionizing radiation; KPNB1 inhibitor importazole reduces both IRF1 protein upregulation and its nuclear localization, thereby attenuating radiation-increased PD-L1 surface expression on tumor cells. siRNA knockdown of IRF1; pharmacological KPNB1 inhibition (importazole); immunofluorescence and Western blot for IRF1 nuclear localization; flow cytometry for surface PD-L1 Current issues in molecular biology Medium 34069326
2022 KPNB1 mediates nuclear import of NFAT5 via direct interaction with a unique NFAT5 nuclear localization signal (NFAT5-NLS), independently of karyopherin α; siRNA screening showed only KPNB1 (not karyopherin α) is required for tonicity-responsive NFAT5 nuclear import. siRNA screening of importin family; Co-IP; nuclear fractionation; NLS mutagenesis Journal of cell science High 35635291
2022 KPNB1 directly interacts with both wild-type and polyQ-expanded ataxin-3 but modulating KPNB1 does not alter ataxin-3 intracellular localization; instead, KPNB1 overexpression reduces ataxin-3 protein levels and aggregate load via protein fragmentation dependent on mitochondrial protease CLPP, improving cell viability in MJD models. Co-immunoprecipitation; label-free quantitative proteomics; KPNB1 knockdown/overexpression; CLPP knockdown; MJD mouse model and iPSC analysis Cellular and molecular life sciences : CMLS Medium 35794401
2022 PCDH1 interacts with KPNB1 and enhances p65 nuclear localization, thereby activating NF-κB signaling to promote pancreatic ductal adenocarcinoma progression. Co-immunoprecipitation (PCDH1-KPNB1); nuclear fractionation of p65; NF-κB reporter assay; cell proliferation/invasion assays Cell death & disease Medium 35864095
2024 USP7 stabilizes KPNB1 through deubiquitination (K48-linked ubiquitination removal); KPNB1 mediates nuclear import of transcription factor YBX1, which then binds the NLGN3 promoter to drive NLGN3 expression and promote glioblastoma progression. Ubiquitination assays; Co-IP/mass spectrometry (KPNB1-YBX1 interaction); nuclear-cytoplasmic fractionation; immunofluorescence; ChIP for YBX1 on NLGN3 promoter; intracranial tumor model Journal of experimental & clinical cancer research : CR High 38254206
2024 TMEM209 binds to KPNB1 and competitively blocks the interaction between KPNB1 and E3 ubiquitin ligase RCHY1, preventing K48-linked ubiquitination-dependent degradation of KPNB1; stabilized KPNB1 then activates the Wnt/β-catenin signaling pathway to promote hepatocellular carcinoma progression. Co-immunoprecipitation (TMEM209-KPNB1 and KPNB1-RCHY1); ubiquitination assays; Western blot; xenograft tumor model Cell death discovery Medium 39414762
2024 KPNB1 specifically recognizes amino acids 280-299 within the NLS of transcription factor ATF4, mediating ATF4 nuclear import; nuclear ATF4 then binds the BNIP3 promoter (at approximately -1292 to -1279 bp and -1185 to -1172 bp) to drive BNIP3-dependent mitophagy and promote odontoblastic differentiation of dental pulp stem cells. IP-mass spectrometry (KPNB1-ATF4); NLS wild-type/mutant plasmid constructs; dual-luciferase reporter and ChIP for ATF4-BNIP3 promoter; nuclear fractionation; mitochondrial function assays; in vivo implantation model Cellular & molecular biology letters High 39604846
2025 KPNB1 directly transports VCP (valosin-containing protein) into the nucleus to support DNA damage repair; withaferin A (WA) covalently binds CYS 158 of KPNB1 to retard VCP nuclear localization and impair DDR. Chemical pulldown combined with immunoprecipitation-mass spectrometry; biochemical Co-IP; covalent binding site mapping (CYS 158 mutagenesis); in vivo xenograft DDR assay Proceedings of the National Academy of Sciences of the United States of America High 40339118
2025 KPNB1 mediates nuclear import of HMGB2 in AML cells; KPNB1 inhibition impairs HMGB2 nuclear entry, leading to compromised DNA damage repair and sensitization of AML cells to venetoclax. Co-immunoprecipitation; nuclear fractionation; siRNA/importazole inhibition; xenograft AML mouse model; patient-derived cell viability assays Oncogene Medium 40082556
2023 RTEL1 interacts with KPNB1 through a distinct C-terminal domain (separate from its NUP153-binding domain), and this interaction connects protein nuclear import to nuclear envelope stability during S-phase; overexpression of the RTEL1 C-terminal domain protects against nuclear envelope anomalies caused by protein import inhibition. Co-immunoprecipitation; domain deletion/truncation mapping; high-resolution microscopy (KPNB1 localization at nuclear pore); nuclear envelope stability assays Cells Medium 38132118
2020 NLS-RARα is transported into the nucleus by binding to both KPNA2 (importin α1) and KPNB1 (importin β1); knockdown of KPNA2/KPNB1 inhibits nuclear accumulation of NLS-RARα, and nuclear NLS-RARα inhibits myeloid cell differentiation. Mass spectrometry of co-immunoprecipitated proteins; Co-IP validation; siRNA knockdown; nuclear/cytoplasmic fractionation; immunofluorescence Xi bao yu fen zi mian yi xue za zhi Medium 32696739
2025 KPNB1 is identified as a reader protein for O-GlcNAcylated RNA Polymerase II CTD (at Ser-5); OGT-mediated O-GlcNAcylation of RNA Pol II promotes its nuclear entry in a KPNB1-dependent manner, connecting metabolic state to global transcription regulation. WGA lectin pulldown combined with mass spectrometry (O-GlcNAcylated CTD interactome); structural modeling; ChIP (wChIP technique); siRNA/inhibitor functional assays bioRxivpreprint Medium
2025 KPNA2/KPNB1 complex sequesters C9orf72 poly(proline-arginine) (polyPR) in the cytoplasm, protecting cells from polyPR nuclear toxicity; genetic silencing of KPNB1 converts PR20-resistant cells to sensitive cells, and KPNB1 overexpression rescues JQ1-induced PR20 nuclear entry and cell death. FITC-labeled PR20 tracking; siRNA knockdown of KPNB1/KPNA2; overexpression rescue; JQ1-mediated KPNB1 downregulation Cell biochemistry and function Medium 39891383
2024 NAT10 upregulates KPNB1 expression through ac4C RNA modification of KPNB1 mRNA; elevated KPNB1 in turn facilitates PD-L1 nuclear translocation to promote immune escape and radiotherapy resistance in NSCLC. NAT10 knockdown; ac4C RNA modification assays; KPNB1 overexpression; nuclear fractionation for PD-L1; immune cell co-culture assays Open life sciences Low 40109769

Source papers

Stage 0 corpus · 38 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2020 KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway. Cell death and differentiation 121 33139930
2017 In vivo loss-of-function screens identify KPNB1 as a new druggable oncogene in epithelial ovarian cancer. Proceedings of the National Academy of Sciences of the United States of America 92 28811376
2013 KPNB1, XPO7 and IPO8 mediate the translocation ofNF-κB/p65 into the nucleus. Traffic (Copenhagen, Denmark) 90 23906023
2014 EZH2-miR-30d-KPNB1 pathway regulates malignant peripheral nerve sheath tumour cell survival and tumourigenesis. The Journal of pathology 58 24132643
2015 KPNB1 mediates PER/CRY nuclear translocation and circadian clock function. eLife 43 26319354
2018 KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells. Oncogene 42 29520102
1998 Strain IMB-1, a novel bacterium for the removal of methyl bromide in fumigated agricultural soils. Applied and environmental microbiology 32 9750123
2015 Antitumor effects of pharmacological EZH2 inhibition on malignant peripheral nerve sheath tumor through the miR-30a and KPNB1 pathway. Molecular cancer 30 25890085
2019 MiR-101 relates to chronic peripheral neuropathic pain through targeting KPNB1 and regulating NF-κB signaling. The Kaohsiung journal of medical sciences 29 30887716
2017 KPNB1-mediated nuclear import is required for motility and inflammatory transcription factor activity in cervical cancer cells. Oncotarget 26 28427184
2019 Targeting KPNB1 overcomes TRAIL resistance by regulating DR5, Mcl-1 and FLIP in glioblastoma cells. Cell death & disease 24 30742128
2017 Decreased KPNB1 Expression is Induced by PLK1 Inhibition and Leads to Apoptosis in Lung Adenocarcinoma. Journal of Cancer 23 29187890
1999 Oxidation of methyl halides by the facultative methylotroph strain IMB-1. Applied and environmental microbiology 20 10543820
2024 Stabilization of KPNB1 by deubiquitinase USP7 promotes glioblastoma progression through the YBX1-NLGN3 axis. Journal of experimental & clinical cancer research : CR 18 38254206
2022 PCDH1 promotes progression of pancreatic ductal adenocarcinoma via activation of NF-κB signalling by interacting with KPNB1. Cell death & disease 18 35864095
2019 Inhibition of KPNB1 Inhibits Proliferation and Promotes Apoptosis of Chronic Myeloid Leukemia Cells Through Regulation of E2F1. OncoTargets and therapy 18 31819526
2001 Identification of methyl halide-utilizing genes in the methyl bromide-utilizing bacterial strain IMB-1 suggests a high degree of conservation of methyl halide-specific genes in gram-negative bacteria. Applied and environmental microbiology 18 11282657
2023 KPNB1-mediated nuclear import in cancer. European journal of pharmacology 17 37473981
2021 CircPITX1 Regulates Proliferation, Angiogenesis, Migration, Invasion, and Cell Cycle of Human Glioblastoma Cells by Targeting miR-584-5p/KPNB1 Axis. Journal of molecular neuroscience : MN 17 33763840
2016 Identification of KPNB1 as a Cellular Target of Aminothiazole Derivatives with Anticancer Activity. ChemMedChem 11 27241026
2021 KPNB1 Inhibitor Importazole Reduces Ionizing Radiation-Increased Cell Surface PD-L1 Expression by Modulating Expression and Nuclear Import of IRF1. Current issues in molecular biology 10 34069326
2015 miR-9 enhances the transactivation of nuclear factor of activated T cells by targeting KPNB1 and DYRK1B. American journal of physiology. Cell physiology 9 25696812
2024 TMEM209 promotes hepatocellular carcinoma progression by activating the Wnt/β-catenin signaling pathway through KPNB1 stabilization. Cell death discovery 7 39414762
2025 NAT10 promotes radiotherapy resistance in non-small cell lung cancer by regulating KPNB1-mediated PD-L1 nuclear translocation. Open life sciences 6 40109769
2022 Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2. Journal of cell science 6 35635291
2023 Human RTEL1 Interacts with KPNB1 (Importin β) and NUP153 and Connects Nuclear Import to Nuclear Envelope Stability in S-Phase. Cells 4 38132118
2025 Targeting KPNB1 suppresses AML cells by inhibiting HMGB2 nuclear import. Oncogene 3 40082556
2025 VCP's nuclear journey: Initiated by interacting with KPNB1 to repair DNA damage. Proceedings of the National Academy of Sciences of the United States of America 3 40339118
2024 Astragaloside Ⅳ mediates the effect and mechanism of KPNB1 on biological behavior and tumor growth in prostate cancer. Heliyon 3 39027542
2024 KPNB1-ATF4 induces BNIP3-dependent mitophagy to drive odontoblastic differentiation in dental pulp stem cells. Cellular & molecular biology letters 3 39604846
2024 KPNA2/KPNB1 promotes the malignant progression of gastric cancer induced by M2 macrophage polarization. Tissue & cell 3 39765137
2015 Up-Regulation of KPNB1 Involves in Neuronal Apoptosis Following Intracerebral Hemorrhage in Adult Rats. Neurochemical research 3 26303509
2024 Targeting KPNB1 with genkwadaphnin suppresses gastric cancer progression through the Nur77-mediated signaling pathway. European journal of pharmacology 2 38823760
2023 MicroRNA-130a-3p impedes the progression of papillary thyroid carcinoma through downregulation of KPNB1 by targeting PSME3. Endocrine 2 37166548
2022 KPNB1 modulates the Machado-Joseph disease protein ataxin-3 through activation of the mitochondrial protease CLPP. Cellular and molecular life sciences : CMLS 2 35794401
2020 [NLS-RARα transports into the nucleus by binding to importin α1/β2(KPNA2/KPNB1) and inhibits the differentiation of U937 cells]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 2 32696739
2025 Super-Enhancer Protects Cells From Toxicity of C9orf72 Poly(proline-arginine) by Inducing the Expression of KPNA2/KPNB1. Cell biochemistry and function 0 39891383
2017 [The nuclear import of Newcastle disease virus matrix protein depends on KPNB1 and Ran protein]. Wei sheng wu xue bao = Acta microbiologica Sinica 0 29746765

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