Affinage

NUP153

Nuclear pore complex protein Nup153 · UniProt P49790

Length
1475 aa
Mass
153.9 kDa
Annotated
2026-06-10
67 papers in source corpus 53 papers cited in narrative 53 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NUP153 is a nuclear-basket nucleoporin that organizes the nucleoplasmic face of the nuclear pore complex (NPC) and serves as a hub coupling nucleocytoplasmic transport to nuclear envelope architecture, mitotic NPC dynamics, and chromatin regulation (PMID:8794857, PMID:9531546). It is targeted to the NPC by its N-terminal domain and exposes an FG/FXFG-repeat C-terminal domain that provides the principal docking sites for transport receptors: it is a major physiological binding site for importin β, accommodates complete importin α/β–NLS import complexes, and carries separable binding sites for the classical and transportin (M9) import pathways, while a zinc-finger Ran-binding motif and RanGTP regulation gate receptor association and direct cargo toward the nuclear interior (PMID:9531546, PMID:9889100, PMID:10202161, PMID:15632149). Through these activities NUP153 mediates import of specific cargos including 53BP1, PU.1, Smad2, and β-catenin and is required for export of mRNA, snRNA, 5S rRNA, and NES-bearing proteins; a conserved N-terminal RNA-binding domain (aa 250–400) binds single-stranded RNA with loose sequence selectivity (PMID:10069809, PMID:11567018, PMID:12191473, PMID:15632149, PMID:22075984, PMID:22110128). NUP153 anchors the basket components Tpr and Nup50 and connects the NPC to the nuclear lamina by binding A- and B-type lamins (PMID:10921874, PMID:12802065, PMID:21983083, PMID:23007389). It drives interphase NPC assembly by binding the inner nuclear membrane via an N-terminal amphipathic helix to recruit the Nup107-160 complex, and participates in mitotic envelope breakdown by recruiting COPI and in post-mitotic envelope reassembly by directing B-type lamin and SUN1 targeting (PMID:26051542, PMID:12967567, PMID:36044344). Beyond transport, NUP153 acts in gene regulation: it binds developmental gene promoters and recruits PRC1 in embryonic stem cells and interacts with Sox2 to shape chromatin accessibility and cell-fate decisions (PMID:26080816, PMID:28919367). NUP153 is a direct docking site for the HIV-1 capsid, engaging the assembled CA lattice through an FG motif and a triple-arginine (RRR) motif at residues lining the capsid hydrophobic pocket (capsid N57), enabling nuclear import of viral cores into non-dividing cells (PMID:19369352, PMID:21593146, PMID:24130490, PMID:36943880). Its abundance is controlled by SPOP–Cul3-mediated ubiquitylation and proteasomal degradation and by sumoylation balanced by SENP1/SENP2 (PMID:39785820, PMID:22688647).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1994 Medium

    Establishing the primary architecture defined NUP153 as an FG-repeat, zinc-finger nucleoporin, framing the structural basis for its later transport and Ran-binding functions.

    Evidence cDNA sequencing and sequence analysis of human NUP153

    PMID:8110839

    Open questions at the time
    • No functional assignment to the repeat or zinc-finger domains
    • No localization data
  2. 1996 High

    Localization to the nuclear basket and domain dissection answered where NUP153 resides and which domain does what, linking N-terminal targeting to NPC anchoring and the C-terminal FG region to mRNA export.

    Evidence GFP/domain overexpression, poly(A)+ in situ hybridization, and subcellular targeting assays in BHK cells

    PMID:8794857

    Open questions at the time
    • Mechanism of FG-dependent export not resolved
    • No receptor partners yet identified
  3. 1999 High

    Identifying importin β, transportin, and Ran interactions established NUP153 as a Ran-regulated docking platform with pathway-specific receptor binding sites, the core of its import function.

    Evidence IP from Xenopus extracts, recombinant binding, dominant-negative fragment inhibition, phage display, GST pulldowns

    PMID:10202161 PMID:9531546 PMID:9889100

    Open questions at the time
    • In vivo cargo specificity not yet defined
    • Stoichiometry of receptor docking at native pores not resolved
  4. 1999 High

    Antibody injection and RNA-binding assays defined NUP153 as required for export of multiple RNA classes and NES-proteins and as an intrinsic RNA-binding nucleoporin, distinguishing it functionally from other Nups.

    Evidence Anti-Nup153 antibody injection in Xenopus oocytes, multi-substrate export assays, homoribopolymer binding

    PMID:10069809 PMID:11567018 PMID:14681581 PMID:17242408

    Open questions at the time
    • Physiological RNA targets in cells not comprehensively mapped
    • Sequence determinants only loosely defined
  5. 2003 High

    Depletion and interaction mapping showed NUP153 anchors Tpr and Nup50 and is required for basket integrity and even NPC distribution, defining its structural-scaffold role.

    Evidence RNAi, immunodepletion/reconstitution in Xenopus, yeast two-hybrid, immunogold EM, domain mapping

    PMID:11598013 PMID:12802065 PMID:23007389

    Open questions at the time
    • How scaffolding feeds back on transport efficiency only partly resolved
  6. 2003 High

    Reconstitution assays placed NUP153 in mitotic and assembly events: it recruits COPI to drive NE breakdown, and binds lamins to couple the NPC to the lamina.

    Evidence Xenopus NE-breakdown assays, dominant-negative perturbation, co-IP, lamin Ig-fold mutant analysis

    PMID:10921874 PMID:12967567 PMID:16314393 PMID:28] placeholder

    Open questions at the time
    • Redundancy with Nup358 in COPI recruitment not fully delineated
    • Lamin-NPC connection mechanism in vivo not fully resolved
  7. 2011 High

    Identification of specific cargos (53BP1, PU.1, Smad2, β-catenin) connected NUP153 transport activity to DNA-damage response and signaling pathways, giving its receptor-docking role physiological output.

    Evidence siRNA screens, co-IP, GST pulldowns, import/export and IR-foci assays

    PMID:12191473 PMID:15632149 PMID:22075984 PMID:22110128

    Open questions at the time
    • Generality of cargo selectivity unclear
    • Direct vs. indirect contributions to 53BP1 function debated across studies
  8. 2015 High

    ChIP-seq and stem-cell assays revealed a transport-independent gene-regulatory role: NUP153 binds promoters, recruits PRC1, and binds Sox2 to control developmental gene expression and cell fate.

    Evidence RNAi/shRNA, ChIP-seq, RNA-seq, ATAC-seq, co-IP, in vivo differentiation assays

    PMID:20174442 PMID:26080816 PMID:28919367

    Open questions at the time
    • Mechanism coupling NPC residence to chromatin binding not resolved
    • Direct vs. indirect promoter binding not fully separated
  9. 2015 High

    Membrane-binding and domain mapping resolved how NUP153 initiates interphase NPC assembly, via an N-terminal amphipathic helix recruiting the Nup107-160 complex.

    Evidence RNAi, in vitro membrane binding, domain mapping, Ran/transportin perturbation

    PMID:26051542

    Open questions at the time
    • Structural basis of helix-membrane insertion not solved
    • Coupling to lipid composition unclear
  10. 2018 High

    Crystallographic and lattice-binding studies defined NUP153 as a direct HIV-1 capsid docking factor, with FG and RRR motifs engaging the CA hexamer/tri-hexamer to enable nuclear import into non-dividing cells.

    Evidence X-ray crystallography, cryo-EM, capsid mutagenesis, competition with PF74/CPSF6, infection assays

    PMID:19369352 PMID:21593146 PMID:24130490 PMID:29997211 PMID:36943880

    Open questions at the time
    • How capsid uncoating couples to NUP153 release not resolved
    • In vivo contribution relative to other Nups not fully quantified
  11. 2025 High

    Identifying SPOP-Cul3 ubiquitylation and SENP1/SENP2-balanced sumoylation established post-translational control of NUP153 abundance, with downstream effects on Mad1 tethering at the NE.

    Evidence Co-IP, ubiquitylation assays, SPOP F102C mutant, RNAi, sumoylation assays, dominant-negative SENPs

    PMID:22688647 PMID:39785820

    Open questions at the time
    • Physiological signals triggering NUP153 turnover unknown
    • Functional consequences of sumoylation cycling not fully defined
  12. 2025 Medium

    Recent work extended NUP153 roles to additional viruses (HBV, orthoflavivirus, SIV/HIV-2 Vpx) and to nuclear migration and mRNA-export machinery, broadening its functional reach.

    Evidence RNAi/CRISPR knockdown, CLIP-seq, mass spectrometry, co-IP, reporter and infection assays, in vivo models

    PMID:31913756 PMID:39666457 PMID:40014546 PMID:40943515 PMID:41951628

    Open questions at the time
    • Mechanistic links to ERK/HNF4α and viral RNA binding are early
    • Tissue-specific significance not established

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NUP153's separable activities—transport docking, basket scaffolding, membrane binding, and chromatin/promoter regulation—are spatially and temporally coordinated within a single protein remains unresolved.
  • No integrated structural model linking domains to all functions
  • Switching between NPC-resident and chromatin-bound pools not understood

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 5 GO:0003723 RNA binding 4 GO:0001618 virus receptor activity 3 GO:0005198 structural molecule activity 2 GO:0140110 transcription regulator activity 2
Localization
GO:0005635 nuclear envelope 4 GO:0000228 nuclear chromosome 3 GO:0005654 nucleoplasm 2
Pathway
R-HSA-1643685 Disease 6 R-HSA-9609507 Protein localization 5 R-HSA-1640170 Cell Cycle 4 R-HSA-73894 DNA Repair 3 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-4839726 Chromatin organization 2 R-HSA-8953854 Metabolism of RNA 2
Partners
IMPORTIN-ΒLMNA/LMNBMAD1NUP50SENP1SPOPTPRTRANSPORTIN-1
Complex memberships
nuclear pore complex (nuclear basket)

Evidence

Reading pass · 53 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 Human NUP153 (hnup153) encodes a nucleoporin with 33 copies of the XFXFG repeat and four zinc finger motifs; the protein bears O-linked N-acetylglucosamine moieties characteristic of nucleoporins. cDNA sequencing and sequence analysis Biochimica et biophysica acta Medium 8110839
1996 Nup153 localizes to the nucleoplasmic face (basket) of the nuclear pore complex; its N-terminal domain is sufficient for NPC targeting (can redirect cytoplasmic pyruvate kinase to the nuclear face); overexpression of Nup153 causes accumulation of nuclear poly(A)+ RNA, indicating inhibition of mRNA export, dependent on the C-terminal FG-repeat domain. Overexpression of GFP/domain constructs in BHK cells, in situ hybridization for poly(A)+ RNA, subcellular targeting assays The Journal of cell biology High 8794857
1998 Nup153 N-terminal domain contains two distinct targeting regions: one for NPC assembly and one for targeting to the inner face of the nuclear envelope; the zinc finger and C-terminal domains have no role in targeting. Deletion analysis with reporter fusion constructs expressed in mammalian cells Chromosoma Medium 9745047
1998 Nup153 and Tpr are the major physiological binding sites for importin β at the nuclear face of the NPC; importin β binds directly to multiple sites in the Nup153 FXFG repeat region; Nup153 can accommodate a complete import complex (importin α, β, and NLS substrate); GMP-PNP disassembles Nup153–importin β complexes. Immunoprecipitation from Xenopus egg extracts and isolated nuclei, direct binding assays with recombinant proteins, competition experiments The Journal of cell biology High 9531546
1998 Nup153 contains separate binding sites for importin α/β (classical NLS import pathway) and transportin (M9 import pathway); dominant-negative Nup153 fragments selectively block one pathway without affecting the other. Dominant-negative fragment inhibition assays in Xenopus import assays, binding studies Current biology High 9889100
1999 Nup153 N-terminus contains an M9 NLS that binds transportin 1 (TRN1); Nup153 interacts with both import and export receptors in a RanGTP-regulated manner (RanGTP dissociates import receptor complexes, but is required for export receptor interactions); Nup153 binds RanGDP via its zinc finger domain (zinc finger Ran-binding motif); Nup153 shuttles between nuclear and cytoplasmic faces of the NPC. Phage display, co-immunoprecipitation, GST pulldown, mobility assays The EMBO journal High 10202161
1999 Nup153 is required for nuclear export of snRNA, mRNA, 5S rRNA, and NES-mediated protein export (HIV Rev pathway), but not for tRNA export or importin β recycling; Nup153 uniquely among tested nucleoporins associates with poly(G) and poly(U) RNA in vitro. Anti-Nup153 antibody injection into Xenopus oocytes, export assays for multiple RNA/protein classes, RNA-binding assays with homoribopolymers Molecular biology of the cell High 10069809
2000 Nup153 is incorporated into the nuclear envelope at the same time as lamina assembly during nuclear reconstitution; lamin B3 co-immunoprecipitates with Nup153 and interacts specifically with its C-terminal domain; blocking lamina assembly prevents Nup153 NE recruitment; disrupting pre-assembled lamina displaces Nup153 but not other nucleoporins. Cell-free Xenopus egg extract nuclear assembly assays, co-immunoprecipitation, dominant-negative lamin mutant (XlaminBΔ2+) The EMBO journal High 10921874
2000 Nup153, along with RanBP2, emerin, and LBR, is recruited to reforming nuclear envelopes early in telophase (5 min after anaphase onset) prior to recovery of nuclear import function (8 min), establishing its role in early NPC reassembly. Live fluorescence imaging of GFP-tagged proteins and immunofluorescence at timed intervals in HeLa cells Journal of cell science Medium 10671368
2001 Nup153 depletion during nuclear reconstitution causes loss of several nuclear basket components, uneven NPC distribution in the NE, and NPC mobility within the NE; importin α/β-mediated import is strongly reduced (due to defective import complex translocation, not receptor recycling), while transportin-mediated import is unaffected. Nup153 immunodepletion from Xenopus egg extracts, nuclear reconstitution, immunogold electron microscopy, nuclear import assays The EMBO journal High 11598013
2001 A conserved RNA binding domain mapping to amino acids 250–400 within the Nup153 N-terminal region directly binds RNA and associates with endogenous RNA targets; this domain is functionally conserved across Drosophila, Xenopus, and human Nup153. Domain deletion/mapping with recombinant fragments, RNA-binding assays in vitro, cross-species comparison The Journal of biological chemistry Medium 11567018
2002 Smad2 directly interacts with nucleoporins CAN/Nup214 and Nup153; these interactions mediate constitutive nucleocytoplasmic shuttling of Smad2; Nup153 competes with the cytoplasmic retention factor SARA and nuclear partner FAST-1 for binding to the hydrophobic corridor on the MH2 surface of Smad2; TGFβ receptor phosphorylation does not alter Smad2 affinity for Nup153. Co-immunoprecipitation, GST pulldown with purified proteins, nuclear import/export assays Molecular cell High 12191473
2003 Nup153 directly binds to Tpr; cellular depletion of Nup153 by RNAi mislocalizes Tpr to the nuclear interior; the Nup153-Tpr interaction is sensitive to specific Tpr amino acid substitution mutations; Nup153 depletion also mislocalizes Nup50 but not other nucleoporins. RNAi depletion, affinity chromatography, yeast two-hybrid, sequential NPC assembly analysis Molecular biology of the cell High 12802065
2003 Nup153 recruits the COPI coatomer complex to the nuclear membrane during mitosis, directing nuclear envelope breakdown; COPI plays a role in NE breakdown via vesiculation. Xenopus in vitro nuclear envelope breakdown assay, co-immunoprecipitation, dominant-negative experiments Developmental cell High 12967567
2004 The Nup153 RNA binding domain (aa 250-400) preferentially associates with single-stranded RNA with little sequence preference, as demonstrated by testing a range of RNA substrates with different structural features. In vitro RNA binding assays with recombinant Nup153 domain and diverse RNA substrates RNA Medium 14681581
2004 Nup153 and Nup98 exchange dynamically on and off the NPC in a transcription-dependent manner; inhibition of Pol I and Pol II transcription blocks Nup153 exchange; distinct domains within Nup153 link its mobility to different RNA polymerases. FRAP (fluorescence recovery after photobleaching) with GFP-Nup153, transcription inhibition, domain mapping Molecular biology of the cell Medium 14718558
2005 The Nup153 FXFG repeat region mediates nuclear entry of BR granule mRNPs into the nuclear basket; entry into the basket is a two-step process: mRNP first binds to basket fibril tips, then is transferred into the basket in a Nup153-dependent step. Anti-Nup153 antibody injection into Chironomus tentans salivary glands, electron microscopy tracking of Balbiani ring granules Molecular biology of the cell Medium 16195343
2005 PU.1 nuclear import requires energy but not soluble carriers; PU.1 interacts directly with Nup153 and Nup62; binding of PU.1 to Nup153 (but not Nup62) increases dramatically in the presence of RanGTP, forming a PU.1-RanGTP-Nup153 complex; RanGTP propels PU.1 toward the nuclear side of the NPC by increasing its affinity for Nup153. GST pulldown, nuclear import assays in permeabilized cells, ultrastructural immunogold localization, RanGMPPNP competition The Journal of biological chemistry High 15632149
2005 Nup358 zinc finger domain binds COPI and inhibits nuclear envelope breakdown; both Nup153 and Nup358 play non-redundant roles in COPI recruitment to the nuclear rim during NE breakdown; a single zinc finger is the minimal interface for COPI association, though tandem zinc fingers are optimal. Xenopus in vitro NE breakdown assay, dominant-negative zinc finger domain expression, antibody inhibition, COPI recruitment assays Molecular biology of the cell Medium 16314393
2007 Nup153 RNA binding domain discriminates between RNA targets based on a loose sequence motif rather than general single-stranded RNA preference; specific subregions of a cellular mRNA account for its association with Nup153. Systematic RNA mutagenesis, in vitro binding assays with synthetic RNA oligonucleotides and recombinant Nup153 RNA-binding domain The Journal of biological chemistry Medium 17242408
2007 In Drosophila, Nup153 FG repeats are specifically required for importin α/β-mediated nuclear import; the remainder of the protein maintains pore integrity; Nup153 depletion selectively impairs import but not CRM1-dependent export. RNAi knockdown in Drosophila S2 cells, import/export functional assays, domain rescue experiments The Journal of cell biology Medium 17682050
2009 HIV-1 integrase (IN) binds directly to the FxFG-rich C-terminal domain of NUP153 (NUP153C); NUP153C added in excess inhibits nuclear import of IN; known NUP153C binding partners compete with IN for NUP153 binding and inhibit IN nuclear import; overexpression of NUP153C in cells reduces HIV vector infectivity by blocking nuclear translocation of viral cDNA. Semipermeabilized cell nuclear import assay, direct binding (GST pulldown/co-immunoprecipitation), competition assays, infectivity assays Journal of virology High 19369352
2009 Nup153 depletion reduces HIV-1 2-LTR circles moderately and integrated proviruses substantially, suggesting NUP153 acts at the nuclear import step of HIV-1 PIC; capsid mutations N74D and P90A render HIV-1 insensitive to NUP153 depletion; simultaneous depletion of NUP153 and TNPO3 yields synergistic inhibitory effects. siRNA knockdown, quantitative PCR for viral replication intermediates (late RT products, 2-LTR circles, integrated proviruses), chimeric MLV/HIV-1 viruses Journal of virology High 21593146
2009 Nup153 depletion causes a delay in late mitosis with increased unresolved midbodies; more severe depletion causes a pronounced early mitotic defect and accumulation of cells with multilobed nuclei; the FG-rich region of Nup153 is required to rescue defects in late mitosis but not the multilobed nuclei phenotype, indicating two separable Nup153 functions in mitosis. siRNA knockdown in HeLa cells, live cell imaging, rescue with FG-domain mutants Molecular biology of the cell Medium 19158386
2010 Nup153 and Megator (Mtor) bind to ~25% of the Drosophila genome in continuous domains (NARs); these domains are enriched for active transcription marks (high RNA Pol II, H4K16ac); RNAi knockdown of Nup153 alters expression of ~5,700 genes with pronounced downregulation within NARs; Nup153 depletion abolishes dosage compensation complex function on the male X chromosome; chromatin binding is independent of sub-nuclear localization. ChIP-chip (chromatin immunoprecipitation with microarray), RNAi knockdown, 3D imaging PLoS genetics High 20174442
2010 Nup153 interacts directly with Mad1 (spindle assembly checkpoint protein) via Nup153's N-terminal domain; Nup153 overexpression causes multinucleated cells, multipolar spindles, and spindle checkpoint inactivation via Mad1 hypophosphorylation; Nup153 depletion reduces Mad1 at nuclear pores and delays Mad1 dissociation from kinetochores, keeping the spindle checkpoint active. In vitro binding assays, overexpression/RNAi in HeLa cells, immunofluorescence, phosphorylation analysis Nucleus Medium 21327106
2010 Nup153 depletion by RNAi alters nuclear lamina organization and Sun1 localization, and causes cytoskeletal rearrangement that impairs cell migration in human breast carcinoma cells. RNAi knockdown, immunofluorescence, cell migration assays FEBS letters Medium 20561986
2011 NUP153 is specifically required for nuclear import of 53BP1; NUP153 knockdown prevents 53BP1 from entering nuclei in newly forming daughter cells, leading to decreased IR-induced 53BP1 foci, delayed DNA repair, and impaired survival after IR; the C-terminal part of NUP153 is required for 53BP1 import through an NUP153–importin-β interplay. siRNA screen, live-cell imaging, co-immunoprecipitation, domain mapping, IR-induced foci assays Cell death and differentiation High 22075984
2011 Nup153 exhibits multiple binding sites for both A-type and B-type lamins; both the N-terminal domain and C-terminal domain of Nup153 directly interact with the Ig-fold domain of lamin A and B; specific mutations in the lamin A Ig-fold domain selectively affect Nup153 binding. GST pulldown assays, blot overlay assays with purified proteins, lamin Ig-fold mutant analysis Nucleus Medium 21983083
2011 β-catenin directly interacts with the FG repeats of Nup153 (as well as Nup62 and Nup98) via specific Arm repeat sequences (R3-8 for import, R10-12 for export/import); knockdown of Nup153 and Nup62 impedes β-catenin nuclear import/export; Tyr-654 phosphorylation stimulates Arm R10-12 transport activity. FRAP assays in live cells, in vitro binding assays with purified components, siRNA knockdown, proteomics The Journal of biological chemistry Medium 22110128
2012 The Nup153–Nup50 interface involves a dual site: (1) Nup50 N-terminal domain binds a unique N-terminal region of Nup153 critical for Nup50 NPC localization; (2) a second site at the distal Nup153 tail depends on importin α; disruption of the Nup153–Nup50 interface decreases nuclear import efficiency. Domain deletion mapping, co-immunoprecipitation, NPC localization assays, import efficiency assays The Journal of biological chemistry Medium 23007389
2012 Nup153 binds both SUMO proteases SENP1 and SENP2 via two distinct sites (N-terminal domain and C-terminal FG-rich region); Nup153 is itself a substrate for sumoylation, kept in check by SENP1 and SENP2; depletion of SENP1/SENP2 or dominant-negative mutants increase Nup153 sumoylation; SENP1 levels are influenced by Nup153 abundance, but SENP2 is not. Co-immunoprecipitation, domain mapping, RNAi, sumoylation assays, dominant-negative SENP constructs Nucleus Medium 22688647
2013 The HIV-1 capsid (CA) N-terminal domain directly interacts with the FG-repeat-enriched Nup153 C-terminal domain (NUP153C); different FG motifs mediate binding to HIV-1 versus EIAV capsids; HIV-1 CA binding maps to residues lining the α-helix 3/4 hydrophobic pocket (Asn57 critical); this pocket is shared with PF74 inhibitor and CPSF6; PF74 and CPSF6 compete with NUP153C for binding to the HIV-1 CA pocket; NUP153C-CA interaction underlies HIV-1 infection of non-dividing cells. Trim-NUP153C restriction assay, in vitro binding with purified proteins, mutagenesis, competition assays with PF74/CPSF6, infection assays in dividing/non-dividing cells PLoS pathogens High 24130490
2015 Nup153 depletion in mouse embryonic stem cells causes derepression of developmental genes and early differentiation without defects in nuclear import of pluripotency factors; Nup153 binds around transcriptional start sites of developmental genes and mediates recruitment of polycomb-repressive complex 1 (PRC1) to a subset of its target loci. RNAi depletion, ChIP-seq, RNA-seq, nuclear import assays for pluripotency factors Genes & development High 26080816
2015 Nup153 is required for interphase NPC assembly but not for post-mitotic NPC reassembly; Nup153 binds directly to the inner nuclear membrane via an N-terminal amphipathic helix during interphase, facilitating recruitment of the Nup107-160 complex to NPC assembly sites; transportin and Ran regulate Nup153–membrane interaction. RNAi depletion, in vitro membrane binding assays, domain mapping, fluorescence microscopy, Ran/transportin perturbation Developmental cell High 26051542
2016 Prelamin A accumulation causes mislocalization of NUP153, disrupts the Ran gradient, and thereby impairs nuclear import of 53BP1, resulting in DNA damage response defects in vascular smooth muscle cells; NUP153 is important for nuclear localization of Ran. siRNA knockdown, immunofluorescence, nuclear fractionation, 53BP1 import assays in cells expressing prelamin A Aging cell Medium 27464478
2017 Nup153 interacts with Sox2 in adult neural progenitor cells; depletion of Nup153 disrupts Sox2 genomic localization and open chromatin configurations at Nup153 target genes; Nup153 binding to gene promoters correlates with increased expression, while binding to transcriptional end sites correlates with decreased expression; Nup153 depletion promotes gliogenic fate switch in vivo. Co-immunoprecipitation, ChIP-seq, ATAC-seq, shRNA knockdown, in vivo neural progenitor differentiation assays Cell stem cell High 28919367
2017 Nup153 and Nup50 promote 53BP1 recruitment to DNA damage foci by antagonizing BRCA1-dependent events; the requirement for Nup153 in 53BP1 foci formation is abrogated in BRCA1/BARD1-deficient cells but not BRCA2-deficient cells, placing Nup153 in a pathway that counteracts BRCA1 activity. siRNA knockdown, 53BP1 foci assays with etoposide/olaparib, genetic epistasis with BRCA1/BARD1/BRCA2 depletion Journal of cell science Medium 28751496
2017 Nup153 depletion results in reduced SUMO1 modification of 53BP1 and displacement of SENP1 from NPCs; artificial NPC tethering of SENP1 restores NHEJ and 53BP1 sumoylation in Nup153-depleted cells; Nup153 is specifically required for NHEJ (not HR through SENP1), while Tpr contributes to both NHEJ and HR, and Nup50 only to NHEJ. siRNA knockdown, SUMO modification assays, NHEJ/HR reporter assays, forced NPC targeting of SENP1 Journal of cell science Medium 28576968
2018 Crystal structures of hexameric HIV-1 capsid in complex with NUP153-derived FG peptides reveal that capsid N57 residue is critical for NUP153 interaction; HIV-1 viruses with N57 substitutions infect dividing but not non-dividing cells, blocking nuclear translocation while allowing reverse transcription; N57 mutant capsids lose interaction with both Nup153 and CPSF6; small molecules PF74 and BI-2 prevent FG-nucleoporin interactions with HIV-1 core. X-ray crystallography, capsid mutagenesis, infection assays in dividing/non-dividing cells, viral replication intermediate quantification, integration site analysis Journal of virology High 29997211
2018 Seh1 (SEH1L) is required for association of both the GATOR2 complex and Nup153 with mitotic chromosomes, but not for the Nup107 complex association with mitotic chromosomes. Chemical genetics (auxin-inducible degron), quantitative chromosome proteomics Journal of cell science Medium 29618633
2020 Vpx of SIVsmPBj1.9 physically interacts with NUP153; MAPK/ERK2-mediated phosphorylation of Vpx is required for its interaction with NUP153; MAPK/ERK2-packaging defective SIV fails to efficiently import its viral genome into nuclei; Vpx-NUP153 interaction is evolutionarily conserved in SIV isolates and HIV-2. Co-immunoprecipitation, superresolution structured illumination microscopy, viral infection assays with MAPK/ERK2-deficient virions Molecular biology of the cell Medium 31913756
2020 Mad1 depletion delays recruitment of Nup153 to anaphase chromatin; the Nup153-Mad1 interaction requires a nuclear envelope to be present; both Nup153 and Mad1 depletion alter NE architecture (membrane curvature at NPCs, expanded inner-outer membrane spacing); Nup153 depletion causes interphase NPC assembly defects with cytoplasmic nucleoporin displacement, but Mad1 depletion does not. RNAi depletion, time-lapse microscopy, electron microscopy, 3D-SIM, proximity ligation assay Journal of cell science Medium 33023979
2022 Nup153 disruption during telophase interferes with ongoing addition of B-type lamins, lamin B receptor, and SUN1 to the expanding NE, but minimally affects lamin A and SUN2 targeting, revealing two functionally separable phases of NE formation and a role for Nup153 in recruiting a specific subset of NE proteins. Nup153 functional disruption, live-cell imaging, immunofluorescence during telophase Molecular biology of the cell Medium 36044344
2022 NUP153 promotes HCC cell proliferation via the c-Myc/P15 axis: NUP153 knockdown upregulates P15INK4b and downregulates c-Myc; c-Myc overexpression partially reverses P15 upregulation and G1/S arrest caused by NUP153 silencing. siRNA knockdown, rescue with c-Myc overexpression, qRT-PCR, Western blot, cell cycle analysis Digestive and liver disease Low 35288064
2023 NUP153 engages the assembled HIV-1 CA lattice through a bipartite motif: a canonical FG motif targeting the CA hexamer, and a novel triple-arginine (RRR) motif targeting the CA tri-hexamer interface; both motifs contribute to HIV-1 nuclear import; NUP153 stabilizes tubular CA assemblies in vitro. Cryo-EM/X-ray crystallography of CA lattice-NUP153 complexes, mutagenesis, HIV-1 infection assays, in vitro CA assembly stabilization assay Proceedings of the National Academy of Sciences of the United States of America High 36943880
2023 RTEL1 interacts with NUP153 via a distinct C-terminal domain, and this interaction can promote nuclear internalization of peptides that diffuse through the nuclear pore; RTEL1 depletion leads to nuclear envelope destabilization in S-phase cells. Co-immunoprecipitation, domain mapping, peptide nuclear import assays, RTEL1 knockout/knockdown, high-resolution microscopy Cells Low 38132118
2024 Nup153 is the anchor for kinesin Kif1a at the nuclear envelope in radial glial progenitors, mediating G1-specific basal nuclear migration (interkinetic nuclear migration); identified by co-immunoprecipitation and functional rescue experiments. Co-immunoprecipitation, RNAi knockdown, live imaging of nuclear migration in brain slices Cell reports Medium 39666457
2025 SPOP (Cul3 substrate adaptor) directly binds Nup153 in a complex, multivalent interaction, and targets it for ubiquitylation and proteasomal degradation; SPOP substrate-binding mutant (F102C) fails to degrade Nup153; RNAi depletion of SPOP stabilizes Nup153; loss of SPOP activity strengthens Mad1 localization at the nuclear envelope (which is tethered there by Nup153). Co-immunoprecipitation, ubiquitylation assays, RNAi depletion, SPOP mutant (F102C) expression, Mad1 localization assays Molecular biology of the cell High 39785820
2025 NUP153 promotes HBV replication by enhancing HBV core promoter activity through upregulation of HNF4α via ERK signaling; NUP153 knockdown inhibits HBV replication without affecting cccDNA levels; NUP153 overexpression increases cccDNA transcription and virion production. RNAi knockdown, CRISPR/Cas9 knockout, luciferase reporter assays, cytoplasmic/nuclear fractionation, mouse hydrodynamic injection model Journal of medical virology Medium 40014546
2025 NUP153 is recruited to the orthoflavivirus amplification site on the ER; NUP153 interacts with viral proteins NS3 and NS5 and a conserved G-rich motif on viral RNA; these interactions specifically promote structural viral protein production, leading to efficient virion assembly and release. Fluorescence microscopy, knockdown, crosslinking immunoprecipitation sequencing (CLIP-seq), mass spectrometry, in vitro and biophysical binding assays Nature communications High 41951628
2024 Nup153 and TPR are required for TREX-2-dependent export of hsp70 mRNA in Drosophila; Nup153 knockdown causes TPR relocation to the nucleoplasm; Nup153 depletion causes TREX-2 subunits to relocate from nuclear pores to the nucleoplasm; both nucleoporins are required for TREX-2 subunit association with nuclear pores. RNAi knockdown, mRNA export assays, immunofluorescence localization of TREX-2 subunits International journal of molecular sciences Medium 40943515
2024 Nup153 docks splicing machinery to the nuclear pore; BioID proximity labeling reveals enrichment of spliceosome complex components (E, A, B, B*, P) near Nup153; splicing components' presence at NPC is reduced upon splicing inhibition and depends partly on Nup153; Nup153-bound genes (~500) show Nup153-dependent splicing defects, suggesting co-transcriptional splicing at the NPC. BioID proximity labeling, in situ proximity ligation assay, STED microscopy, DamID, RNAi knockdown with splicing assays bioRxivpreprint Medium bio_10.1101_2024.09.30.615666

Source papers

Stage 0 corpus · 67 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2013 Nucleoporin NUP153 phenylalanine-glycine motifs engage a common binding pocket within the HIV-1 capsid protein to mediate lentiviral infectivity. PLoS pathogens 250 24130490
2002 Smad2 nucleocytoplasmic shuttling by nucleoporins CAN/Nup214 and Nup153 feeds TGFbeta signaling complexes in the cytoplasm and nucleus. Molecular cell 207 12191473
2010 Nuclear pore proteins nup153 and megator define transcriptionally active regions in the Drosophila genome. PLoS genetics 205 20174442
1999 Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain. The EMBO journal 198 10202161
2011 The requirement for nucleoporin NUP153 during human immunodeficiency virus type 1 infection is determined by the viral capsid. Journal of virology 188 21593146
2001 The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins. The EMBO journal 188 11598013
1996 Targeting and function in mRNA export of nuclear pore complex protein Nup153. The Journal of cell biology 167 8794857
1998 Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr. The Journal of cell biology 166 9531546
2000 Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes. Journal of cell science 160 10671368
2003 Direct interaction with nup153 mediates binding of Tpr to the periphery of the nuclear pore complex. Molecular biology of the cell 153 12802065
2015 The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing. Genes & development 128 26080816
1999 The nucleoporin nup153 plays a critical role in multiple types of nuclear export. Molecular biology of the cell 119 10069809
2000 Incorporation of the nuclear pore basket protein nup153 into nuclear pore structures is dependent upon lamina assembly: evidence from cell-free extracts of Xenopus eggs. The EMBO journal 118 10921874
2015 Nup153 Recruits the Nup107-160 Complex to the Inner Nuclear Membrane for Interphasic Nuclear Pore Complex Assembly. Developmental cell 117 26051542
1998 Separate nuclear import pathways converge on the nucleoporin Nup153 and can be dissected with dominant-negative inhibitors. Current biology : CB 108 9889100
2017 Nup153 Interacts with Sox2 to Enable Bimodal Gene Regulation and Maintenance of Neural Progenitor Cells. Cell stem cell 96 28919367
2004 Distinct functional domains within nucleoporins Nup153 and Nup98 mediate transcription-dependent mobility. Molecular biology of the cell 95 14718558
2018 Nup153 Unlocks the Nuclear Pore Complex for HIV-1 Nuclear Translocation in Nondividing Cells. Journal of virology 94 29997211
2009 Integrase interacts with nucleoporin NUP153 to mediate the nuclear import of human immunodeficiency virus type 1. Journal of virology 90 19369352
2005 Versatility at the nuclear pore complex: lessons learned from the nucleoporin Nup153. Chromosoma 74 16133350
2011 Distinct association of the nuclear pore protein Nup153 with A- and B-type lamins. Nucleus (Austin, Tex.) 71 21983083
2009 The nucleoporin Nup153 has separable roles in both early mitotic progression and the resolution of mitosis. Molecular biology of the cell 68 19158386
2011 Specific armadillo repeat sequences facilitate β-catenin nuclear transport in live cells via direct binding to nucleoporins Nup62, Nup153, and RanBP2/Nup358. The Journal of biological chemistry 65 22110128
2003 The COPI complex functions in nuclear envelope breakdown and is recruited by the nucleoporin Nup153. Developmental cell 65 12967567
2011 Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1. Cell death and differentiation 62 22075984
2012 The Nup153-Nup50 protein interface and its role in nuclear import. The Journal of biological chemistry 51 23007389
2007 Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport. The Journal of cell biology 51 17682050
2010 The nucleoporin Nup153 affects spindle checkpoint activity due to an association with Mad1. Nucleus (Austin, Tex.) 50 21327106
2010 The nucleoporin Nup153 maintains nuclear envelope architecture and is required for cell migration in tumor cells. FEBS letters 48 20561986
2016 Prelamin A impairs 53BP1 nuclear entry by mislocalizing NUP153 and disrupting the Ran gradient. Aging cell 46 27464478
1998 Amino-terminal sequences that direct nucleoporin nup153 to the inner surface of the nuclear envelope. Chromosoma 43 9745047
2005 Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules. Molecular biology of the cell 41 16314393
1994 Sequence analysis of a cDNA encoding a human nuclear pore complex protein, hnup153. Biochimica et biophysica acta 41 8110839
2023 The capsid lattice engages a bipartite NUP153 motif to mediate nuclear entry of HIV-1 cores. Proceedings of the National Academy of Sciences of the United States of America 39 36943880
2012 Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2. Nucleus (Austin, Tex.) 38 22688647
2005 Carrier-independent nuclear import of the transcription factor PU.1 via RanGTP-stimulated binding to Nup153. The Journal of biological chemistry 38 15632149
2019 Altered Nup153 Expression Impairs the Function of Cultured Hippocampal Neural Stem Cells Isolated from a Mouse Model of Alzheimer's Disease. Molecular neurobiology 35 30689197
2005 The functionally conserved nucleoporins Nup124p from fission yeast and the human Nup153 mediate nuclear import and activity of the Tf1 retrotransposon and HIV-1 Vpr. Molecular biology of the cell 35 15659641
2014 Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy. Nucleus (Austin, Tex.) 33 25485891
2005 Nup153 affects entry of messenger and ribosomal ribonucleoproteins into the nuclear basket during export. Molecular biology of the cell 32 16195343
2017 Localisation of Nup153 and SENP1 to nuclear pore complexes is required for 53BP1-mediated DNA double-strand break repair. Journal of cell science 31 28576968
2001 RNA association defines a functionally conserved domain in the nuclear pore protein Nup153. The Journal of biological chemistry 20 11567018
2017 Nup153 and Nup50 promote recruitment of 53BP1 to DNA repair foci by antagonizing BRCA1-dependent events. Journal of cell science 19 28751496
2000 Recombinant Nup153 incorporates in vivo into Xenopus oocyte nuclear pore complexes. Journal of structural biology 17 10806081
2018 Seh1 targets GATOR2 and Nup153 to mitotic chromosomes. Journal of cell science 16 29618633
2007 Multiple conserved domains of the nucleoporin Nup124p and its orthologs Nup1p and Nup153 are critical for nuclear import and activity of the fission yeast Tf1 retrotransposon. Molecular biology of the cell 14 17615301
2007 Sequence preference in RNA recognition by the nucleoporin Nup153. The Journal of biological chemistry 13 17242408
2004 The RNA binding domain within the nucleoporin Nup153 associates preferentially with single-stranded RNA. RNA (New York, N.Y.) 11 14681581
2022 NUP153 promotes HCC cells proliferation via c-Myc-mediated downregulation of P15INK4b. Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 9 35288064
2022 A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents. Molecular biology of the cell 9 36044344
2020 Mitotic checkpoint protein Mad1 is required for early Nup153 recruitment to chromatin and nuclear envelope integrity. Journal of cell science 6 33023979
2025 Cul3 substrate adaptor SPOP targets Nup153 for degradation. Molecular biology of the cell 4 39785820
2024 Nup153 is not required for anchoring heterochromatic DSBs to the nuclear periphery. microPublication biology 4 38737725
2023 Human RTEL1 Interacts with KPNB1 (Importin β) and NUP153 and Connects Nuclear Import to Nuclear Envelope Stability in S-Phase. Cells 4 38132118
2021 Expression of NR5A2, NUP153, HNF4A, USP15 and FNDC3B is consistent with their use as novel biomarkers for bovine mammary stem/progenitor cells. Journal of molecular histology 4 33400051
2020 Viral protein X unlocks the nuclear pore complex through a human Nup153-dependent pathway to promote nuclear translocation of the lentiviral genome. Molecular biology of the cell 4 31913756
2025 Nup358 and Nup153 Facilitate nuclear import of BmNPV nucleocapsids in Bombyx mori cells. Journal of invertebrate pathology 3 40120667
2022 Effect of Genetic Polymorphism Including NUP153 and SVEP1 on the Pharmacokinetics and Pharmacodynamics of Ticagrelor in Healthy Chinese Subjects. Clinical drug investigation 3 35501592
2025 The role of ABI2 in modulating nuclear proteins: Therapeutic implications for NUP54 and NUP153 in TNBC. Advances in protein chemistry and structural biology 2 39843146
2025 Novel Function of NUP153 in HNF4α Transcriptional Upregulation Contributes to Promoting HBV Replication. Journal of medical virology 2 40014546
2025 The functional and clinical significance of nucleoporin NUP153 across human cancers: a systematic study based on multi-omics analysis and bench work validation. Frontiers in immunology 2 40607419
2026 Proviral NUP153 binding to viral proteins and RNA regulates structural-nonstructural protein ratios in orthoflavivirus infection. Nature communications 0 41951628
2026 Progerin-induced nuclear envelope remodeling is shaped by cell division and NUP153. Journal of cell science 0 42237879
2025 Nup153 and TPR/Megator Interact with TREX-2 Subunits and Are Essential for TREX-2-Dependent Nuclear Export of hsp70 mRNA in Drosophila. International journal of molecular sciences 0 40943515
2024 The nucleoporin Nup153 is the anchor for Kif1a during basal nuclear migration in brain progenitor cells. Cell reports 0 39666457
2023 Expression of the NUP153 and YWHAB genes from their canonical promoters and alternative promoters of the LINE-1 retrotransposon in the placenta of the first trimester of pregnancy. Vavilovskii zhurnal genetiki i selektsii 0 36923475
2023 Cul3 substrate adaptor SPOP targets Nup153 for degradation. bioRxiv : the preprint server for biology 0 37293018

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