Affinage

RANBP1

Ran-specific GTPase-activating protein · UniProt P43487

Length
201 aa
Mass
23.3 kDa
Annotated
2026-04-28
56 papers in source corpus 31 papers cited in narrative 31 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RANBP1 is a cytoplasmic co-activator of the Ran GTPase cycle that governs nucleocytoplasmic transport and mitotic spindle assembly. It binds Ran-GTP with nanomolar affinity via recognition of the Ran C-terminal acidic tail, accelerates the off-rate of Ran-GTP from importin-β–family receptors and CRM1 export complexes by >100-fold to generate a transient Ran-GTP·RanBP1 intermediate that is then hydrolyzed by RanGAP1, and inhibits RCC1-mediated nucleotide exchange on Ran (PMID:7882974, PMID:9428644, PMID:10330396, PMID:31021318). During mitosis, RanBP1 forms a trimeric RCC1/Ran/RanBP1 complex that spatially controls RCC1 chromatin association and thereby shapes the Ran-GTP gradient required for spindle assembly factor localization, chromosome segregation, and post-mitotic nuclear reformation; loss of Ranbp1 in mice causes microcephaly owing to impaired cortical progenitor M-phase progression (PMID:25458009, PMID:32594833, PMID:17940066, PMID:25452572). RanBP1 also regulates cell polarity and directed migration by controlling nuclear export of the kinase LKB1 through the CRM1 pathway (PMID:30184488, PMID:36206829).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1995 High

    Identification of RanBP1 as a new class of GTPase regulator resolved how Ran-GTP hydrolysis is accelerated beyond RanGAP1 alone, establishing the co-activator paradigm for Ran cycling.

    Evidence In vitro GTPase, nucleotide-exchange, and affinity-purification assays with recombinant proteins; Ran C-terminal mutagenesis

    PMID:7782302 PMID:7882974

    Open questions at the time
    • No structural basis for the co-activation mechanism at this stage
    • Whether RanBP1 operates on karyopherin-bound Ran was untested
  2. 1996 High

    Discovery of the cytoplasmic localization signal (NES) in RanBP1 and the trimeric RanBP1·Ran·importin-β complex explained how spatial restriction of RanBP1 to the cytoplasm drives directional transport.

    Evidence Co-IP from HeLa extracts, recombinant reconstitution, permeabilized-cell import assay; NES deletion/mutation with GFP-tagged constructs and LMB treatment

    PMID:8794858 PMID:8909533

    Open questions at the time
    • Export receptor for the RanBP1 NES not yet identified as CRM1
    • How the NES-dependent cytoplasmic pool is maintained quantitatively was unclear
  3. 1997 High

    Quantitative kinetic analysis established RanBP1 as the principal disassembly factor for receptor–RanGTP complexes, increasing the off-rate >100-fold across multiple importin-β family members and CRM1, and defined the transient RanGTP·RanBP1 intermediate as the substrate for RanGAP1.

    Evidence In vitro kinetic binding/hydrolysis assays with importin-β, CAS, exportin; yeast complementation of yrb1(ts); ternary complex reconstitution; biophysical measurements (SPR, fluorescence); NES-CRM1 export reporter assays

    PMID:10660567 PMID:8995296 PMID:9111043 PMID:9271386 PMID:9315840 PMID:9428644

    Open questions at the time
    • Crystal structure of RanBP1 in complex with Ran and a karyopherin not yet obtained
    • Relative contributions of RanBP1 vs. RanBP2 in vivo were unresolved
  4. 1997 High

    The trimeric RCC1/Ran/RanBP1 complex was identified and shown to be functionally required for both nuclear assembly and transport, revealing that RanBP1 balances RCC1 activity to maintain Ran-GTP homeostasis.

    Evidence Xenopus egg extract immunodepletion and add-back; in vitro binding and exchange assays; yeast overexpression toxicity in RCC1-deficient background

    PMID:7616957 PMID:9348536

    Open questions at the time
    • Whether RanBP1 regulates RCC1 chromatin association was unknown
    • Phospho-regulation of this complex was not addressed
  5. 1999 High

    RanBP1 was shown to promote the terminal step of CRM1-mediated nuclear export by releasing CRM1 from cytoplasmic nucleoporins (Nup214/Can), establishing RanBP1's role at the cytoplasmic face of the NPC.

    Evidence Permeabilized-cell export reconstitution with RanQ69L, nucleoporin fractionation, immunofluorescence

    PMID:10330396

    Open questions at the time
    • Whether RanBP1 acts via direct contact with CRM1 or solely through Ran sequestration was unresolved
  6. 2000 High

    RanBP1 was found to shuttle between nucleus and cytoplasm by a non-classical, Ran-GTP–dependent mechanism, and yeast complementation proved that co-activation of RanGAP is the essential biological function, not Ran-GTP binding per se.

    Evidence LMB treatment, microinjection, dominant-negative Ran mutants, RanBP1 point mutants in permeabilized cells; random mutagenesis with yeast complementation

    PMID:10660567 PMID:10779340

    Open questions at the time
    • Import receptor for RanBP1 not identified
    • Regulation of shuttling dynamics under physiological conditions uncharacterized
  7. 2007 High

    Loss-of-function in human cells revealed that RanBP1 is required for proper spindle dynamics and chromosome segregation, specifically for recruiting cyclin B1 and positioning HURP at microtubule plus-ends.

    Evidence RNAi knockdown in human cells with immunofluorescence, spindle stability assay, chromosome segregation scoring

    PMID:17940066

    Open questions at the time
    • Whether RanBP1 acts through modulating the Ran-GTP gradient or through a spindle-local mechanism was unclear
    • Centrosome-localized fraction's function not delineated
  8. 2014 High

    The RCC1/Ran/RanBP1 trimeric complex was shown to spatially control RCC1 chromatin dynamics and Ran-GTP gradient magnitude during mitosis, and Ranbp1 knockout mice demonstrated an essential role in cortical progenitor M-phase progression and brain development.

    Evidence Xenopus egg extract FRAP and spindle assembly assays; targeted Ranbp1−/− mouse with BrdU/EdU proliferation and cortical layer analysis

    PMID:25452572 PMID:25458009

    Open questions at the time
    • RanBP1 phosphorylation sites controlling the metaphase-anaphase transition not fully mapped
    • Whether microcephaly phenotype is solely mitotic or also involves transport defects was unresolved
  9. 2018 Medium

    RanBP1 was placed upstream of a polarity signaling cascade (LKB1→STK25→GM130) that controls Golgi orientation and axonogenesis, extending its function beyond transport/mitosis to cell polarity.

    Evidence RNAi in cultured neurons and in vivo electroporation; LKB1 localization and axon morphometry

    PMID:30184488

    Open questions at the time
    • Direct physical interaction between RanBP1 and LKB1 not demonstrated
    • Whether RanBP1 regulates LKB1 export through CRM1 directly or indirectly was unclear
  10. 2019 High

    Comparative reconstitution resolved a long-standing question: animal RanBP1 disassembles export complexes by sequestering RanGTP (forming a 1:1:2 CRM1:RanBP1:RanGTP complex) rather than by directly contacting CRM1 as in fungi, explaining evolutionary divergence in export complex disassembly.

    Evidence In vitro reconstitution with size-exclusion chromatography, binding assays, comparative mutagenesis between animal and fungal orthologs

    PMID:31021318

    Open questions at the time
    • Structural model of the animal 1:1:2 complex not yet available
    • Whether RanBP2 uses the same or different mechanism in animals not tested
  11. 2020 High

    Acute degradation of RanBP1 in human somatic cells confirmed that its regulation of RCC1 chromatin dynamics and HURP localization during mitosis is a conserved mechanism, not limited to embryonic extracts.

    Evidence Auxin-induced degron for acute RanBP1 depletion, FRAP/FLIP of RCC1-GFP, HURP immunofluorescence in human cells

    PMID:32594833

    Open questions at the time
    • How RanBP1 degradation at telophase is regulated remains uncharacterized
    • Downstream spindle assembly factors beyond HURP not systematically assessed
  12. 2022 Medium

    RanBP1 was shown to regulate directed collective migration of neural crest cells via LKB1 nuclear export and to physically interact with CD147, linking it to microtubule stability and paclitaxel sensitivity.

    Evidence Morpholino knockdown in Xenopus neural crest with chemotaxis assay; Co-IP/FRET/SPR of CD147–RanBP1 interaction with microtubule dynamics readout

    PMID:34974521 PMID:36206829

    Open questions at the time
    • CD147–RanBP1 interaction not validated in non-cancer cell types
    • How RanBP1 simultaneously coordinates transport, polarity, and microtubule dynamics is not integrated
  13. 2023 Medium

    Oxidative stress was found to trigger EGFR/PKA-dependent nuclear accumulation of RanBP1 via phosphorylation at Ser60 and Tyr103, identifying a stress-responsive regulatory input into RanBP1 localization.

    Evidence EGFR/PKA inhibitors, site-directed mutagenesis of Ser60/Tyr103, subcellular fractionation under oxidative stress

    PMID:38011756

    Open questions at the time
    • Direct kinase-substrate relationship (EGFR or PKA phosphorylating Ser60/Tyr103) not demonstrated with purified kinases
    • Functional consequences of stress-induced nuclear RanBP1 accumulation on transport or mitosis not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis of the animal-specific 1:1:2 CRM1:RanBP1:RanGTP disassembly complex, how RanBP1 degradation timing at telophase is controlled, the identity of RanBP1's nuclear import receptor, and whether RanBP1's roles in polarity and microtubule regulation are mechanistically separable from its canonical transport-cycle function.
  • No high-resolution structure of animal RanBP1 in an export disassembly complex
  • Import receptor for RanBP1 remains unidentified
  • Integration of transport, mitotic, and polarity functions into a unified regulatory model is lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 6 GO:0060090 molecular adaptor activity 3
Localization
GO:0005829 cytosol 3 GO:0005634 nucleus 2 GO:0005815 microtubule organizing center 1
Pathway
R-HSA-9609507 Protein localization 6 R-HSA-1640170 Cell Cycle 4 R-HSA-1266738 Developmental Biology 2
Partners
BSGKPNB1RANRANBP5RANGAP1RCC1XPO1
Complex memberships
RCC1/Ran/RanBP1 trimeric complexRanBP1/Ran/importin-β trimeric complex

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 RanBP1 (23 kDa) binds tightly to Ran-GTP but not Ran-GDP, co-activates RanGAP1-induced GTP hydrolysis on Ran by ~10-fold, inhibits RCC1-stimulated nucleotide exchange on Ran-GTP, and forms a stable complex with nucleotide-free RCC1-Ran; it represents a new class of GTPase regulators distinct from GDIs. In vitro GTPase assay, nucleotide exchange assay, affinity purification, protein biochemistry The EMBO journal High 7882974
1995 The acidic C-terminal -DEDDDL sequence of Ran is required for high-affinity interaction with RanBP1 (HTF9A); without this domain, RanBP1 acts as a RanGAP inhibitor rather than co-activator, and the domain undergoes a nucleotide-dependent conformational change. In vitro binding assay, site-directed mutagenesis of Ran C-terminus, GTPase assays The Journal of biological chemistry High 7782302
1996 RanBP1 forms a trimeric complex with Ran and p97 (importin beta) in HeLa cell extracts and when reconstituted from recombinant proteins; RanBP1 increases the affinity of both Ran-GDP and Ran-GTP for p97 to the same level and stabilizes receptor-pore interactions, stimulating nuclear protein import. Co-immunoprecipitation from HeLa extracts, gel filtration, recombinant reconstitution, permeabilized cell import assay The Journal of cell biology High 8909533
1996 RanBP1 is primarily cytoplasmic due to a C-terminal nuclear export signal (NES); the isolated Ran-binding domain (RBD) accumulates in the nucleus, and the NES is necessary (but not sufficient) for nuclear export of a functional RBD. Cytoplasmic RanBP1 promotes glucocorticoid receptor nuclear import whereas isolated RBD inhibits it. Subcellular fractionation, transient transfection with GFP/epitope-tagged constructs, microinjection, leptomycin B treatment, nuclear export signal deletion/mutation The Journal of cell biology High 8794858
1997 RanBP1 is the key disassembly factor for receptor-RanGTP complexes (importin beta, CAS/CSE1L, exportin): RanBP1 increases the off-rate at the receptor/RanGTP interface by >100-fold, generating a transient RanGTP·RanBP1 complex that is then acted upon by RanGAP to hydrolyze GTP, preventing receptor rebinding. Release of importin beta from RanGTP additionally requires importin alpha. In vitro kinetic binding assays, nucleotide hydrolysis assay, reconstitution with purified components FEBS letters High 9428644
1997 RanBP1 forms a ternary complex with Ran and karyopherin beta (importin beta) at distinct non-competing sites on Ran; RanBP1 partially rescues karyopherin beta-mediated inhibition of RanGAP activity through a combination of competitive and noncompetitive interactions, enabling GTP hydrolysis on Ran complexed to importin beta. Solution binding assays, RanGAP activity kinetics, ternary complex reconstitution The Journal of biological chemistry High 8995296
1997 The NES in RanBP1 is functional and can replace the Rev NES, mediating CRM1-dependent nuclear export; mutations inactivating the RanBP1 NES cause nuclear retention and abolish Rev-mediated HIV-1 post-transcriptional regulation, indicating RanBP1 and Rev share the same CRM1 export pathway. NES mutagenesis, Rev-reporter assay in transfected cells, subcellular localization by immunofluorescence The Journal of biological chemistry Medium 9111043
1997 RanBP1 and RCC1 exist in a co-depletion complex in Xenopus egg extracts; the balance between RanBP1 and RCC1 is critical for nuclear assembly and nuclear transport—restoring only one of the two to co-depleted extracts inhibits these processes, while restoring both rescues them. Xenopus egg extract immunodepletion, nuclear assembly assay, nuclear import assay, DNA replication assay, add-back of recombinant proteins Molecular biology of the cell High 9348536
1997 RanBP1 binds RCC1 only in the presence of Ran (forming a trimeric complex), and inhibits RCC1-stimulated nucleotide release from Ran in vitro; overexpression of RanBP1 is selectively toxic to RCC1-deficient cells, confirming negative regulation of RCC1 in vivo. Two-hybrid, in vitro binding, in vitro nucleotide exchange assay, yeast and mammalian cell overexpression Molecular & general genetics Medium 7616957
1997 Two distinct but overlapping binding domains for Ran-GTP and Ran-GDP/RanBP1 exist on p97 (importin beta); an acidic sequence and Cys-158 in p97 are specifically required for Ran-GDP/RanBP1 binding but not for Ran-GTP binding, and the Cys-158 mutant cannot support nuclear import. Site-directed mutagenesis and deletion analysis of p97, in vitro binding assays, permeabilized cell import assay The Journal of biological chemistry High 9045717
1997 RanBP1 binds RanBP5 (importin beta-related) as part of a trimeric RanBP1-Ran-RanBP5 complex; RanBP1 can relieve RanBP5-mediated GAP resistance of RanGTP, suggesting a general role in disassembling importin beta-family/Ran complexes. Yeast two-hybrid with RanBP1 as bait, overlay assay, in vitro GTPase assays, gel filtration Molecular and cellular biology Medium 9271386
1997 Kinetic and equilibrium analysis shows RanBP1 binds Ran-GTP with nanomolar affinity, while Ran-GDP binding is ~10 µM (largely due to a dramatically increased dissociation rate constant); Ran lacking its C-terminal five residues (RanΔC) loses nanomolar affinity for RanBP1 in GTP-bound state. Complex formation induces significant secondary structure changes. Fluorescence spectroscopy with fluorescent GTP analogues, surface plasmon resonance (BIAcore), circular dichroism Biochemistry High 9315840
1999 RanBP1 promotes the terminal step of nuclear export by releasing CRM1 from the cytoplasmic face of the nuclear pore complex (NPC), where CRM1 accumulates in association with Nup214/Can and the p62 complex in a RanGTP-dependent manner; RanBP2 Ran-binding domains have the same activity. Permeabilized cell nuclear export reconstitution, dominant-negative RanQ69L pre-incubation, biochemical fractionation, in vitro nucleoporin binding assay, immunofluorescence The Journal of cell biology High 10330396
1999 Conformational analysis by 31P NMR shows Ran-GTP exists in two slowly interconverting states; RanBP1 binding stabilizes one conformational state (state 2) of Ran-GTP, which corresponds to the GTP-bound form seen in the Ran-RanBP1 crystal structure. 31P NMR spectroscopy, comparison of wild-type and mutant Ran Biochemistry Medium 10471274
2000 RanBP1 shuttles between nucleus and cytoplasm by an active, non-classical mechanism requiring Ran-GTP for nuclear accumulation (not a classical NLS-dependent import); leptomycin B causes nuclear accumulation, low temperature blocks import, and nuclear Ran-GTP is required for translocation. A RanBP1 E37K mutant cannot import into the nucleus despite forming ternary complexes with Ran and importin beta. Leptomycin B treatment, microinjection, permeabilized cell assay, dominant-negative Ran mutants, RanBP1 point mutants, fluorescence microscopy Molecular and cellular biology High 10779340
2000 The essential biological activity of RanBP1 in yeast correlates with its capacity to potentiate RanGAP-mediated GTP hydrolysis on Ran complexed to karyopherins, not with Ran-GTP binding per se or formation of ternary complexes; truncated RanBP1 lacking its NES still complements yrb1(ts) lethality. Random mutagenesis, in vitro biochemical assays (binding, RanGAP stimulation), yeast complementation of yrb1 temperature-sensitive mutant The Journal of biological chemistry High 10660567
2001 Importin beta binding to RanGTP induces exposure of the Ran C-terminal tail and stimulates RanBP1 binding; the basic patch (HRKK142) of Ran is required for CRM1 binding but its mutation increases RanBP1 affinity and decreases importin beta binding, indicating distinct and partially competing binding interfaces on Ran. Limited proteolysis/protection assay, solution binding, mutant Ran proteins Journal of molecular biology Medium 11124902
2001 XMog1, in combination with RanBP1, promotes selective GTP loading onto Ran and facilitates RCC1-catalysed nucleotide exchange from Ran-GDP to Ran-GTP; neither protein alone has this activity, revealing a cooperative mechanism for nuclear Ran-GTP generation. In vitro nucleotide exchange and GTPase assays, Xenopus oocyte two-hybrid, co-expression rescue of S. pombe mog1 mutant Journal of cell science Medium 11686304
2002 FRET biosensor analysis in intact cells detects a cytoplasmic Ran-GDP·RanBP1·importin beta ternary complex; importin beta, RanBP1, and CRM1 complexes with Ran-GTP all show reduced FRET consistent with C-terminal tail extension of Ran, indicating tail displacement is a general mechanism facilitating RanBP1 binding to karyopherin-Ran complexes. FRET with GFP-Ran and Alexa546, microinjection into living cells, purified protein in vitro FRET The Journal of biological chemistry Medium 12034733
2003 RanBP1 overexpression in mammalian cells causes loss of centrosome cohesion during mitosis, specifically splitting of mother and daughter centrioles, which individually organize functional spindle poles, leading to multipolar spindles; this requires microtubule integrity and Eg5 activity, and a fraction of RanBP1 localizes to the centrosome. Overexpression, immunofluorescence, centrosome co-localization, pharmacological inhibition (taxol, monastrol), live cell imaging Journal of cell science Medium 12840069
2005 RanGAP1 is phosphorylated in vivo and in vitro by casein kinase II (CK2) at Serine-358; although this phosphorylation does not alter GAP catalytic activity, it stabilizes formation of a ternary complex of RanGAP1 with Ran and RanBP1 in vivo. MALDI-TOF-MS phosphosite identification, site-directed mutagenesis, in vitro kinase assay with purified CK2, kinase inhibitors (DRB, apigenin), co-immunoprecipitation Cell structure and function Medium 16428860
2007 RANBP1 depletion in human cells yields hyperstable mitotic spindles that fail to recruit cyclin B1 and mis-localize HURP (DLG7) away from microtubule plus-ends; RANBP1-depleted cells show lagging chromosomes in anaphase consistent with unresolved merotelic attachments, indicating RANBP1 is required for proper localization of microtubule-regulatory factors on spindles. RNAi knockdown, immunofluorescence, mitotic spindle stability assay, chromosome segregation analysis Journal of cell science High 17940066
2010 RanBP1 downregulation at mid-to-late telophase is required for nuclear reformation after mitosis; mild RanBP1 overexpression causes persistence of RanBP1 in late mitosis, blocking chromatin decondensation, nuclear expansion, nuclear lamina reorganisation, nuclear pore reassembly, and NLS cargo nuclear relocalisation; co-expression of importin beta mitigates these defects. Overexpression, RNAi, immunofluorescence time-course through mitosis, NLS cargo import assay, nuclear lamina staining Chromosoma Medium 20658144
2014 RanBP1 forms a heterotrimeric RCC1/Ran/RanBP1 complex in M-phase Xenopus egg extracts; this complex controls RCC1 enzymatic activity and its partitioning between chromatin-bound and soluble pools, thereby defining the spatial distribution and magnitude of Ran-GTP gradients that guide mitotic spindle assembly. RanBP1 phosphorylation drives changes in chromatin-bound RCC1 dynamics at the metaphase-anaphase transition. Xenopus egg extract biochemistry, immunoprecipitation, FRAP, spindle assembly assay, phosphorylation analysis Developmental cell High 25458009
2014 Loss of Ranbp1 in mice selectively impairs M-phase progression of cortical progenitors, reduces basal progenitor populations at E14.5, and substantially diminishes layer 2/3 (but not 5/6) cortical projection neurons, causing microcephaly and exencephaly, demonstrating a required mitotic role for RanBP1 in cortical neurogenesis. Targeted mouse knockout (Ranbp1-/-), BrdU/EdU proliferation assays, immunofluorescence of progenitor markers, cell cycle analysis Cerebral cortex High 25452572
2018 RanBP1 regulates the cytoplasmic levels of the polarity kinase LKB1/Par4 via nuclear export machinery; downstream of RanBP1, LKB1 signals through the STK25-GM130 pathway to control Golgi orientation and axonogenesis, establishing RanBP1 as a regulator of cortical neuron polarity and radial migration. RNAi knockdown in cultured neurons and in vivo electroporation, immunofluorescence, LKB1 localization assay, axon/dendrite morphometry Cell reports Medium 30184488
2019 Animal RanBP1 disassembles nuclear export complexes by sequestering RanGTP away from export complexes (not by direct CRM1-RanBP1 interaction as in fungi); animal RanBP1, CRM1 and RanGTP form a 1:1:2 complex (two RanGTP per complex) whereas fungal counterparts form 1:1:1; the mechanistic switch is due to loss of conserved residues mediating RanBP1-CRM1 interaction in animals. In vitro reconstitution, size exclusion chromatography, binding assays, comparative mutagenesis between fungal and animal proteins eLife High 31021318
2020 RanBP1 controls mitotic RCC1 dynamics (chromatin-bound vs. soluble pools) in human somatic cells (not only embryonic systems); RanBP1 degradation via auxin-induced degron alters HURP relocalization in metaphase cells, confirming that RanBP1-dependent RCC1 regulation functionally modulates spindle assembly factor activities in somatic human cells. Auxin-induced degron (AID) for acute RanBP1 degradation, FRAP/FLIP of RCC1-GFP, immunofluorescence of HURP Cell cycle High 32594833
2022 RanBP1 is required for directed (front-to-rear polarity-dependent) collective migration of neural crest cells; RanBP1 function in chemotaxis involves regulated nuclear export of LKB1/PAR4, placing RanBP1 in a polarity pathway upstream of LKB1 during directed cell migration. Morpholino knockdown in Xenopus neural crest, chemotaxis assay, LKB1 localization assay, rescue experiments Developmental biology Medium 36206829
2022 CD147 interacts with RanBP1 through its intracellular domain (CD147ICD) binding the C-terminal tail of RanBP1, as shown by Co-IP, FRET, and SPR; this interaction mediates CD147-regulated microtubule stability and dynamics and determines response to paclitaxel. Co-immunoprecipitation, FRET, surface plasmon resonance (SPR), FRAP of microtubule dynamics, truncation analysis Oncogene Medium 34974521
2023 Oxidative stress causes nuclear accumulation of RanBP1 via EGFR and PKA signaling pathways; pharmacological inhibition of EGFR or PKA diminishes oxidant-induced RanBP1 nuclear relocation; Ser60 and Tyr103 of RanBP1 are required for nuclear accumulation during oxidant exposure, while cysteine residues are not essential. Oxidative stress treatment, EGFR/PKA inhibitors, site-directed mutagenesis of RanBP1 (Ser60, Tyr103, cysteines), subcellular fractionation and immunofluorescence European journal of cell biology Medium 38011756

Source papers

Stage 0 corpus · 56 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. The EMBO journal 342 7882974
1997 RanBP1 is crucial for the release of RanGTP from importin beta-related nuclear transport factors. FEBS letters 222 9428644
1999 A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export. The Journal of cell biology 181 10330396
1996 RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import. The Journal of cell biology 164 8909533
1996 A nuclear export signal is essential for the cytosolic localization of the Ran binding protein, RanBP1. The Journal of cell biology 135 8794858
1997 Ran-binding protein 1 (RanBP1) forms a ternary complex with Ran and karyopherin beta and reduces Ran GTPase-activating protein (RanGAP) inhibition by karyopherin beta. The Journal of biological chemistry 123 8995296
1995 The C terminus of the nuclear RAN/TC4 GTPase stabilizes the GDP-bound state and mediates interactions with RCC1, RAN-GAP, and HTF9A/RANBP1. The Journal of biological chemistry 109 7782302
1997 Different binding domains for Ran-GTP and Ran-GDP/RanBP1 on nuclear import factor p97. The Journal of biological chemistry 82 9045717
1997 Ran-binding protein 5 (RanBP5) is related to the nuclear transport factor importin-beta but interacts differently with RanBP1. Molecular and cellular biology 75 9271386
2003 Mammalian RanBP1 regulates centrosome cohesion during mitosis. Journal of cell science 73 12840069
2000 Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1. Molecular and cellular biology 69 10779340
1997 Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1. The Journal of biological chemistry 56 9111043
2012 Sgk1 enhances RANBP1 transcript levels and decreases taxol sensitivity in RKO colon carcinoma cells. Oncogene 54 23108393
1997 Dynamic and equilibrium studies on the interaction of Ran with its effector, RanBP1. Biochemistry 54 9315840
2007 RANBP1 localizes a subset of mitotic regulatory factors on spindle microtubules and regulates chromosome segregation in human cells. Journal of cell science 52 17940066
1997 Expression of the murine RanBP1 and Htf9-c genes is regulated from a shared bidirectional promoter during cell cycle progression. The Biochemical journal 49 9224656
2014 Ranbp1, Deleted in DiGeorge/22q11.2 Deletion Syndrome, is a Microcephaly Gene That Selectively Disrupts Layer 2/3 Cortical Projection Neuron Generation. Cerebral cortex (New York, N.Y. : 1991) 45 25452572
1998 EVH1/WH1 domains of VASP and WASP proteins belong to a large family including Ran-binding domains of the RanBP1 family. FEBS letters 42 9883880
2014 RanBP1 governs spindle assembly by defining mitotic Ran-GTP production. Developmental cell 40 25458009
1999 Conformational states of the nuclear GTP-binding protein Ran and its complexes with the exchange factor RCC1 and the effector protein RanBP1. Biochemistry 36 10471274
1997 The balance of RanBP1 and RCC1 is critical for nuclear assembly and nuclear transport. Molecular biology of the cell 36 9348536
2002 Fluorescence resonance energy transfer biosensors that detect Ran conformational changes and a Ran x GDP-importin-beta -RanBP1 complex in vitro and in intact cells. The Journal of biological chemistry 35 12034733
1997 Deregulated expression of the RanBP1 gene alters cell cycle progression in murine fibroblasts. Journal of cell science 34 9410874
1995 RanBP1, a Ras-like nuclear G protein binding to Ran/TC4, inhibits RCC1 via Ran/TC4. Molecular & general genetics : MGG 34 7616957
2009 RanBP1 downregulation sensitizes cancer cells to taxol in a caspase-3-dependent manner. Oncogene 32 19270727
1995 The RCC1 protein interacts with Ran, RanBP1, hsc70, and a 340-kDa protein in Xenopus extracts. The Journal of biological chemistry 31 7738003
2017 SGK1 affects RAN/RANBP1/RANGAP1 via SP1 to play a critical role in pre-miRNA nuclear export: a new route of epigenomic regulation. Scientific reports 30 28358001
1999 Two E2F sites control growth-regulated and cell cycle-regulated transcription of the Htf9-a/RanBP1 gene through functionally distinct mechanisms. The Journal of biological chemistry 26 10187822
1998 A RanBP1 mutation which does not visibly affect nuclear import may reveal additional functions of the ran GTPase system. Experimental cell research 25 9770360
2010 Nuclear reformation after mitosis requires downregulation of the Ran GTPase effector RanBP1 in mammalian cells. Chromosoma 20 20658144
2002 RanBP1, a velocardiofacial/DiGeorge syndrome candidate gene, is expressed at sites of mesenchymal/epithelial induction. Mechanisms of development 20 11804793
2000 Random mutagenesis and functional analysis of the Ran-binding protein, RanBP1. The Journal of biological chemistry 20 10660567
2018 RanBP1 Couples Nuclear Export and Golgi Regulation through LKB1 to Promote Cortical Neuron Polarity. Cell reports 18 30184488
2023 RANBP1 (RAN Binding Protein 1): The Missing Genetic Piece in Cancer Pathophysiology and Other Complex Diseases. Cancers 17 36672435
2021 RANBP1 promotes colorectal cancer progression by regulating pre-miRNA nuclear export via a positive feedback loop with YAP. Oncogene 17 34615998
2020 RanBP1 controls the Ran pathway in mammalian cells through regulation of mitotic RCC1 dynamics. Cell cycle (Georgetown, Tex.) 16 32594833
2005 Phosphorylation of RanGAP1 stabilizes its interaction with Ran and RanBP1. Cell structure and function 16 16428860
2001 A role for the basic patch and the C terminus of RanGTP in regulating the dynamic interactions with importin beta, CRM1 and RanBP1. Journal of molecular biology 16 11124902
2001 Ran binding protein RanBP1 in zebrafish embryonic development. Molecular reproduction and development 16 11424209
2001 XMog1, a nuclear ran-binding protein in Xenopus, is a functional homologue of Schizosaccharomyces pombe mog1p that co-operates with RanBP1 to control generation of Ran-GTP. Journal of cell science 16 11686304
2019 Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals. eLife 15 31021318
1998 Interactions with single-stranded and double-stranded DNA-binding factors and alternative promoter conformation upon transcriptional activation of the Htf9-a/RanBP1 and Htf9-c genes. The Journal of biological chemistry 14 9417108
2022 CD147 supports paclitaxel resistance via interacting with RanBP1. Oncogene 12 34974521
2022 RanBP1 plays an essential role in directed migration of neural crest cells during development. Developmental biology 10 36206829
2019 Nicotiana benthamiana RanBP1-1 Is Involved in the Induction of Disease Resistance via Regulation of Nuclear-Cytoplasmic Transport of Small GTPase Ran. Frontiers in plant science 9 30906303
2023 RanBP1: A Potential Therapeutic Target for Cancer Stem Cells in Lung Cancer and Glioma. International journal of molecular sciences 8 37047826
1999 Isolated mammalian and Schizosaccharomyces pombe ran-binding domains rescue S. pombe sbp1 (RanBP1) genomic mutants. Molecular biology of the cell 8 10397757
2023 RANBP1, a member of the nuclear-cytoplasmic trafficking-regulator complex, is the terminal-striking point of the SGK1-dependent Th17+ pathological differentiation. Frontiers in immunology 7 37441077
2023 Oxidative stress and signaling through EGFR and PKA pathways converge on the nuclear transport factor RanBP1. European journal of cell biology 4 38011756
2025 RANBP1 Regulates NOTCH3-Mediated Autophagy in High Glucose-Induced Vascular Smooth Muscle Cells. Frontiers in bioscience (Landmark edition) 3 40018934
2023 Ranbp1 modulates morphogenesis of the craniofacial midline in mouse models of 22q11.2 deletion syndrome. Human molecular genetics 3 36790128
2001 Molecular cloning of CaYRB1, the Candida albicans RanBP1/YRB1 homologue. Yeast (Chichester, England) 2 11447597
2024 Unveiling the Movement of RanBP1 During the Cell Cycle and Its Interaction with a Cyclin-Dependent Kinase (CDK) in Plants. International journal of molecular sciences 1 39795905
2025 The role of RANBP1 in regulating MiRNA expression and apoptosis in breast cancer cells. Genes & genomics 0 40839321
2025 RANBP1 promotes immune evasion in triple-negative breast cancer by suppressing T cell infiltration via the miR-769-5p/PRUNE2 axis. Discover oncology 0 41186818
2024 Quantitative proteomic landscape of the pathophysiology of adhesive arachnoiditis and its clinical significance: Structure and mechanism of TNC and RANBP1 proteins. International journal of biological macromolecules 0 39662555