Affinage

RANBP1

Ran-specific GTPase-activating protein · UniProt P43487

Length
201 aa
Mass
23.3 kDa
Annotated
2026-06-10
57 papers in source corpus 37 papers cited in narrative 37 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RanBP1 is a conserved cytoplasmic Ran-GTP-binding protein that defines a distinct class of Ran GTPase regulators governing the directionality of the Ran nucleotide cycle and, through it, nucleocytoplasmic transport and mitotic spindle assembly (PMID:7882974). It binds Ran-GTP with nanomolar affinity through a Ran-binding domain that recognizes the acidic C-terminal -DEDDDL tail of Ran, co-activates RanGAP1-mediated GTP hydrolysis ~10-fold, and inhibits RCC1-catalyzed nucleotide exchange, forming a stable RCC1/Ran/RanBP1 ternary complex (PMID:7882974, PMID:7782302, PMID:7616957, PMID:9315840). Mechanistically RanBP1 acts as a disassembly factor: it forms transient trimeric complexes with importin-β-family receptors and Ran-GTP, accelerating release of Ran-GTP from transport receptors by >100-fold to be handed to RanGAP for hydrolysis, and in animal cells it dismantles CRM1 export complexes by sequestering Ran-GTP away from CRM1 and promoting CRM1 release from the cytoplasmic face of the nuclear pore (PMID:9428644, PMID:10330396, PMID:31021318). A leucine-rich NES confines RanBP1 to the cytoplasm where it supports nuclear import, and the protein shuttles through the pore via a Ran-GTP-dependent, importin-independent route (PMID:8794858, PMID:10779340). In mitosis RanBP1 controls the spatial Ran-GTP gradient by regulating the activity and chromatin partitioning of RCC1 through the RCC1/Ran/RanBP1 heterotrimer and through phosphorylation at the metaphase-anaphase transition, thereby governing spindle microtubule dynamics, centrosome cohesion, chromosome segregation, and nuclear reformation after mitosis (PMID:17940066, PMID:20658144, PMID:25458009, PMID:32594833, PMID:12840069). RanBP1 abundance is cell-cycle regulated by an S-phase-activated E2F/Sp1 bidirectional promoter, and its level must decline in late mitosis to permit nuclear envelope and NPC reassembly (PMID:9224656, PMID:10187822, PMID:20658144). In the developing nervous system RanBP1 is required for M-phase progression of cortical progenitors and for axon specification and directed migration via nuclear-export control of the polarity kinase LKB1 acting through the STK25-GM130 pathway (PMID:25452572, PMID:30184488, PMID:36206829).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1995 High

    Established the foundational biochemical identity of RanBP1 as a novel Ran regulator that both potentiates RanGAP and antagonizes RCC1, resolving how Ran's GTP/GDP state is biased.

    Evidence In vitro GTPase and nucleotide exchange assays with purified recombinant proteins; two-hybrid and yeast genetics for RCC1 interaction

    PMID:7616957 PMID:7782302 PMID:7882974

    Open questions at the time
    • Did not resolve how these opposing activities are coordinated in cells
    • Structural basis of the Ran C-terminal tail recognition not yet defined
  2. 1996 High

    Showed RanBP1 is not merely a GAP cofactor but a transport-cycle component that forms trimeric complexes with importin-β and Ran and is held cytoplasmic by an NES, linking its biochemistry to nuclear import.

    Evidence Immunoadsorption, gel filtration, recombinant reconstitution, permeabilized cell import assays, and NES deletion/domain-swap localization

    PMID:8794858 PMID:8909533

    Open questions at the time
    • Whether trimer formation drives complex assembly or disassembly was not yet distinguished
    • Kinetics of receptor release not quantified
  3. 1997 High

    Defined RanBP1's core mechanistic role as a disassembly catalyst that strips Ran-GTP from transport receptors and as a thermodynamic switch tuned by Ran's C-terminus.

    Evidence Kinetic dissociation assays with multiple transport receptors, fluorescence/SPR affinity measurements, and ternary-complex reconstitutions including RanBP5 and karyopherin-β

    PMID:8995296 PMID:9045717 PMID:9271386 PMID:9315840 PMID:9428644

    Open questions at the time
    • In vivo confirmation of >100-fold off-rate enhancement was indirect
    • How disassembly is spatially restricted to the cytoplasmic NPC face unaddressed
  4. 1997 Medium

    Connected RanBP1 dosage to cell-cycle physiology, showing its balance with RCC1 and its S-phase-regulated expression are essential for proper nuclear assembly, replication, and mitotic exit.

    Evidence Xenopus egg extract immunodepletion/add-back, promoter dissection with reporter assays, and overexpression phenotyping in fibroblasts

    PMID:9224656 PMID:9348536 PMID:9410874

    Open questions at the time
    • Molecular cause of mitotic-exit defects upon overexpression not yet mechanistic
    • Promoter studies did not link expression timing to functional demand
  5. 1997 Medium

    Extended RanBP1's reach to CRM1-dependent export by showing its NES-dependent cytoplasmic localization intersects the Rev/CRM1 export pathway.

    Evidence NES mutational analysis and Rev/CTE reporter assays in transfected cells

    PMID:9111043

    Open questions at the time
    • Single lab, cell-based assay only
    • Direct biochemical competition with CRM1 not yet demonstrated
  6. 1999 Medium

    Provided structural and reconstitution evidence that RanBP1 promotes CRM1 release from the NPC and induces a defined Ran conformational state, mechanizing its export-terminating role.

    Evidence Permeabilized cell export reconstitution, biochemical fractionation of CRM1-nucleoporin complexes, and 31P NMR of Ran complexes

    PMID:10330396 PMID:10471274

    Open questions at the time
    • Stoichiometry of the RanBP1-CRM1-RanGTP species not yet determined
    • NMR state assignment from a single lab
  7. 2000 High

    Resolved which RanBP1 activity is essential and showed RanBP1 itself shuttles through the pore, separating Ran binding from the catalytically essential GAP-co-activation function.

    Evidence Random mutagenesis correlated across binding/GAP/ternary assays with yeast complementation, plus leptomycin B, microinjection, and permeabilized-cell shuttling assays

    PMID:10397757 PMID:10660567 PMID:10779340

    Open questions at the time
    • Why RanBP1 needs to enter the nucleus mechanistically unclear
    • Discrepancy with yeast (cytoplasmic confinement dispensable) not reconciled
  8. 2001 High

    Clarified the allosteric logic of RanBP1 recruitment, showing importin-β binding exposes Ran's C-terminus to stimulate RanBP1 association and that CRM1 uses distinct Ran determinants.

    Evidence Limited proteolysis protection, solution binding with Ran basic-patch and tail mutants, and FRET tail-extension measurements in vitro and in living cells

    PMID:11124902 PMID:11686304 PMID:12034733

    Open questions at the time
    • In vivo physiological relevance of XMog1 cooperation in mammals untested
    • Quantitative contribution of each Ran determinant to in vivo flux unmeasured
  9. 2007 High

    Established RanBP1's mitotic spindle function, linking its loss to hyperstable microtubules, spindle-factor mislocalization, and chromosome mis-segregation.

    Evidence siRNA knockdown with immunofluorescence and spindle stability assays in human cells; centrosome overexpression phenotyping with Eg5 inhibitor epistasis

    PMID:12840069 PMID:17940066

    Open questions at the time
    • Whether spindle defects derive solely from Ran-GTP gradient disruption not isolated
    • Direct centrosome targets of RanBP1 unknown
  10. 2010 High

    Demonstrated that timely decline of RanBP1 in late mitosis is required for nuclear envelope and NPC reassembly via restoration of importin-β cargo import.

    Evidence Inducible overexpression with temporal microscopy across cell-cycle stages and importin-β co-expression rescue

    PMID:20658144

    Open questions at the time
    • Mechanism degrading RanBP1 at telophase not identified
    • Direct importin-β cargo set affected not enumerated
  11. 2014 High

    Mechanized RanBP1's mitotic role as control of RCC1 chromatin dynamics and the spatial Ran-GTP gradient, and showed organismal requirement in cortical neurogenesis.

    Evidence Xenopus extract heterotrimer reconstitution, chromatin fractionation and phosphorylation analysis; targeted mouse knockout with M-phase and cortical-layer readouts

    PMID:25452572 PMID:25458009

    Open questions at the time
    • Kinase responsible for metaphase-anaphase RanBP1 phosphorylation not identified
    • Link between gradient control and specific spindle assembly factors incomplete
  12. 2019 High

    Revealed an animal-specific export-disassembly mechanism in which RanBP1 sequesters Ran-GTP from CRM1 rather than co-binding CRM1 as in fungi, redefining cross-species mechanistic models.

    Evidence In vitro reconstitution with stoichiometry determination and mutational analysis of interface residues

    PMID:31021318

    Open questions at the time
    • In vivo consequence of the 1:1:2 complex in animal cells not tested
    • Whether divergence affects export cargo selectivity unknown
  13. 2020 High

    Confirmed in human somatic cells via acute depletion that RanBP1 directly governs mitotic RCC1 exchange dynamics and downstream spindle-factor localization.

    Evidence Auxin-inducible degron depletion with FRAP/FLIP of RCC1-GFP and HURP immunolocalization

    PMID:32594833

    Open questions at the time
    • Quantitative Ran-GTP gradient changes inferred, not directly imaged
    • Phosphoregulation in human cells not yet dissected
  14. 2022 Medium

    Identified new physical partners and disease-relevant functions, linking RanBP1 to CD147-regulated microtubule dynamics and to caspase-3-dependent apoptosis and chemosensitivity.

    Evidence Co-IP, FRET, SPR, FRAP and xenografts for CD147; siRNA with caspase-3 epistasis and taxol response across cell lines

    PMID:19270727 PMID:34974521

    Open questions at the time
    • How CD147 binding to the RanBP1 C-terminus alters Ran-cycle activity unresolved
    • Molecular link from RanBP1 loss to caspase-3 activation undefined
  15. 2023 Medium

    Showed RanBP1 localization is signal-regulated, with oxidative stress driving nuclear accumulation through EGFR/PKA signaling and specific phosphosites.

    Evidence Subcellular fractionation, EGFR/PKA pharmacological inhibition, and S60A/Y103F mutational analysis with microscopy

    PMID:38011756

    Open questions at the time
    • Functional consequence of stress-induced nuclear RanBP1 not determined
    • Direct kinase acting on S60/Y103 not identified

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RanBP1's distinct activities—transport-complex disassembly, mitotic RCC1/Ran-GTP gradient control, and neuronal LKB1 export—are differentially deployed and post-translationally switched in space and time remains unresolved.
  • The mitotic kinase and degradation machinery acting on RanBP1 are unidentified
  • Whether a single biochemical activity underlies all phenotypes or separable functions exist is unclear
  • No high-resolution structure of the animal RanBP1-CRM1-RanGTP export complex

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 5 GO:0003924 GTPase activity 3 GO:0060090 molecular adaptor activity 3 GO:0140313 molecular sequestering activity 3
Localization
GO:0005634 nucleus 3 GO:0005829 cytosol 3 GO:0005635 nuclear envelope 2 GO:0005815 microtubule organizing center 1
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-9609507 Protein localization 4 R-HSA-1266738 Developmental Biology 3 R-HSA-5357801 Programmed Cell Death 1
Partners
BSG (CD147)CRM1 (XPO1)IMPORTIN-Β (KPNB1)LKB1 (STK11)RANRANBP5 (IPO5)RANGAP1RCC1
Complex memberships
RCC1/Ran/RanBP1 heterotrimerRanBP1/CRM1/Ran-GTP export complexRanBP1/Ran-GTP/importin-β ternary complexRanGAP1/Ran/RanBP1 ternary complex

Evidence

Reading pass · 37 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 RanBP1 (23 kDa) binds tightly to Ran-GTP but not Ran-GDP, co-activates RanGAP1-induced GTP hydrolysis by ~10-fold, inhibits RCC1-stimulated nucleotide exchange on Ran, and forms a stable complex with nucleotide-free RCC1-Ran. It defines a new class of GTPase regulators distinct from GDIs. In vitro GTPase and nucleotide exchange assays with purified recombinant proteins; biochemical fractionation The EMBO journal High 7882974
1995 The acidic C-terminal -DEDDDL sequence of Ran is required for high-affinity interaction with RanBP1 (HTF9A); deletion of this domain reduces RanBP1 affinity to ~10 µM and converts RanBP1 from a RanGAP co-activator to a RanGAP inhibitor. In vitro binding assays, GTPase activity assays with C-terminal deletion mutants of Ran The Journal of biological chemistry High 7782302
1995 RanBP1 binds RCC1 only in the presence of Ran (forming a trimeric complex) and inhibits RCC1-stimulated guanine nucleotide release from Ran in vitro. Overexpression of RanBP1 is detrimental in RCC1-deficient cells, establishing it as a negative regulator of RCC1. Two-hybrid interaction screen, in vitro GST pulldown, in vitro nucleotide exchange assay, yeast genetics (rcc1 mutant complementation/suppression) Molecular & general genetics : MGG High 7616957
1996 RanBP1 forms a trimeric complex with p97 (importin-β) and Ran, stabilizes the interaction of Ran-GDP with p97, promotes nuclear import by stabilizing receptor docking at the pore, and stimulates translocation in a permeabilized cell import assay. Immunoadsorption from HeLa cell extracts, gel filtration, recombinant protein reconstitution, permeabilized cell import assay The Journal of cell biology High 8909533
1996 RanBP1 contains a leucine-rich nuclear export signal (NES) C-terminal to its Ran-binding domain that is necessary for its cytoplasmic localization; the isolated RBD lacking the NES accumulates in the nucleus. The cytoplasmic localization of RanBP1 is important for nuclear protein import. Subcellular fractionation, transfection of deletion/domain-swap constructs, permeabilized cell assay The Journal of cell biology High 8794858
1997 RanBP1 forms a ternary complex with Ran-GTP and karyopherin beta (importin-β) and partially relieves the complete inhibition of RanGAP activity imposed by karyopherin beta, acting through competitive and non-competitive kinetic mechanisms at distinct sites on Ran. Solution binding assays, kinetic analysis of RanGAP activity in the presence of purified components The Journal of biological chemistry High 8995296
1997 RanBP1 acts as a key disassembly intermediate for importin-β-related transport receptor–RanGTP complexes: RanBP1 stimulates the off-rate of RanGTP from the receptor by >100-fold, transiently releasing RanGTP·RanBP1 which is then driven by RanGAP to hydrolyze GTP. Release of importin-β additionally requires importin-α. In vitro binding and dissociation kinetics with purified transport receptors, RanGTP, and RanBP1; functional nuclear transport assays FEBS letters High 9428644
1997 RanBP1 NES mutations that abolish cytoplasmic localization block Rev-mediated HIV-1 nuclear export, demonstrating that RanBP1 competes with or shares the CRM1-dependent nuclear export pathway used by Rev. This inhibitory effect is independent of RanBP1's ability to bind Ran. Mutational analysis of NES; reporter assays for Rev-mediated and CTE-mediated HIV-1 expression in transfected cells The Journal of biological chemistry Medium 9111043
1997 RanBP1 binds Ran-GTP with nanomolar affinity and Ran-GDP with ~10 µM affinity; the difference is primarily due to a dramatically faster dissociation rate constant for the GDP-bound form. The C-terminal five residues of Ran are required for high-affinity RanBP1 binding to the GTP form. Fluorescence spectroscopy with nucleotide analogues, surface plasmon resonance (BIAcore), circular dichroism Biochemistry High 9315840
1997 RanBP5, identified through a two-hybrid screen using RanBP1 as bait, binds RanBP1 as part of a trimeric RanBP1–Ran–RanBP5 complex; RanBP1 can relieve GAP-resistance of the RanBP5–RanGTP complex. Yeast two-hybrid, overlay assay, in vitro GAP assay with purified components Molecular and cellular biology Medium 9271386
1997 Two distinct but overlapping binding domains for Ran-GTP and Ran-GDP/RanBP1 exist on p97 (importin-β); Cys-158 of p97 is required for Ran-GDP/RanBP1 binding but not Ran-GTP binding, and a Cys158Ala mutant p97 cannot support nuclear import. Site-directed mutagenesis and deletion analysis of p97; permeabilized cell import assay The Journal of biological chemistry High 9045717
1997 The balance between RanBP1 and RCC1 is critical: restoring only one of these two proteins to co-depleted Xenopus egg extracts causes abnormal nuclear assembly and inhibits transport and DNA replication, rescued by addition of the other protein. The GTP/GDP-Ran balance is the essential functional parameter. Immunodepletion from Xenopus egg extracts, recombinant protein add-back, nuclear assembly and import assays Molecular biology of the cell High 9348536
1997 Both mRNA species from the bidirectional Htf9-a/RanBP1 and Htf9-c promoter peak in S phase; cell cycle-dependent transcription is controlled at the transcriptional level by an S-phase-activated bidirectional promoter containing E2F and Sp1 recognition sites. Northern blotting of cell cycle fractions, transient reporter assays with promoter deletion constructs The Biochemical journal Medium 9224656
1997 Deregulated (forced) expression of RanBP1 in murine fibroblasts disrupts cell cycle progression: it inhibits DNA replication, causes defective mitotic exit, and impairs chromatin decondensation at telophase-to-interphase transition. Stable transfection, BrdU incorporation, FACS analysis, microscopy Journal of cell science Medium 9410874
1998 The RanBP1 Ran-binding domain (RBD) belongs structurally to the EVH1/WH1 domain superfamily shared by VASP, WASP, and Homer proteins. Computational sequence/structural analysis (domain recognition) FEBS letters Low 9883880
1998 The distal E2F-b site plus a neighboring Sp1 element actively drive RanBP1 transcriptional upregulation in S phase, while the proximal E2F-c site mediates repression upon growth arrest; each site interacts with distinct E2F family members. Site-directed mutagenesis of promoter E2F sites, transient reporter assays, protein-binding assays The Journal of biological chemistry Medium 10187822
1999 RanBP1 restores nuclear export of NFAT after RanQ69L-induced accumulation of CRM1 at the cytoplasmic face of the NPC, and both RanBP1 and the Ran-binding domains of RanBP2 promote release of CRM1 from the NPC. RanGTP is required for targeting export complexes to the cytoplasmic face of the NPC. Permeabilized cell export reconstitution assay, biochemical fractionation, in vitro reconstitution of CRM1-nucleoporin interactions The Journal of cell biology High 10330396
1999 RanGTP bound to RanBP1 adopts a specific conformational state (state 2) distinct from free Ran-GTP; the RCC1–Ran–nucleotide ternary complex intermediate is detectable by 31P NMR, providing structural evidence for the exchange mechanism. 31P NMR spectroscopy of purified Ran complexes; conformational analysis Biochemistry Medium 10471274
1999 Isolated RBD from mammalian RanBP1 or S. pombe sbp1p is sufficient to rescue growth of sbp1-null fission yeast, and the RBD localizes to the nucleus rather than cytoplasm, indicating that cytoplasmic confinement is not required for essential RanBP1 function in yeast. Genetic complementation of sbp1 null yeast, subcellular localization of exogenous constructs Molecular biology of the cell Medium 10397757
2000 RanBP1 shuttles actively through the nuclear pore: it accumulates in nuclei upon leptomycin B treatment (CRM1 inhibition), its import requires nuclear Ran-GTP (but not classical importin pathway), and an E37K mutation abolishes nuclear accumulation despite preserving ternary complex formation with Ran and importin-β. Leptomycin B treatment, cytoplasmic microinjection, permeabilized cell accumulation assay, nuclear import with exogenous karyopherins and RCC1 Molecular and cellular biology High 10779340
2000 The essential biological activity of RanBP1 in yeast correlates specifically with its capacity to potentiate RanGAP activity toward Ran-GTP within karyopherin complexes, not with Ran-GTP binding per se or ternary complex formation. Mutants crippled for RanGAP co-activation cannot rescue growth even if they form ternary complexes. Random mutagenesis, in vitro biochemical assays (Ran binding, RanGAP stimulation, ternary complex formation), complementation of temperature-sensitive yrb1 yeast The Journal of biological chemistry High 10660567
2001 Importin-β binding to Ran-GTP disrupts an intramolecular interaction between the basic patch (HRKK142) and the C-terminal DEDDDL motif of Ran, exposing the C-terminus and stimulating RanBP1 binding. Mutating the basic patch increases RanBP1 affinity and enables importin-β release without importin-α. CRM1 binding requires the basic patch but uses different Ran determinants than importin-β. Limited proteolysis protection assays, solution binding measurements with Ran mutants, in vitro dissociation assays Journal of molecular biology High 11124902
2001 XMog1 (Xenopus Mog1) co-operates with RanBP1 to promote selective GTP loading onto Ran from GDP, and to facilitate RCC1-catalyzed generation of Ran-GTP in the nucleus; alone, neither protein is sufficient for this activity. Two-hybrid screening, in vitro nucleotide exchange and GTPase assays with purified components, yeast genetic rescue Journal of cell science Medium 11686304
2002 FRET measurements show that binding of RanGTP to importin-β, RanBP1, or CRM1 all extend the C-terminal tail of Ran (reduced FRET between N-terminal GFP and C-terminal Alexa546). A Ran-GDP·RanBP1·importin-β ternary complex with extended tail is detected both in vitro and in intact cells via cytoplasmic FRET. FRET using Ran-GFP labeled with Alexa546; co-injection into living cells; in vitro reconstitution The Journal of biological chemistry High 12034733
2003 RanBP1 overexpression specifically in mitosis induces splitting of mother and daughter centrioles at spindle poles, generating multipolar spindles; this requires microtubule integrity and Eg5 activity. A fraction of RanBP1 localizes at the centrosome during mitosis. Overexpression in mammalian cells, microscopy (centrosome and spindle staining), drug treatments (nocodazole, monastrol) Journal of cell science Medium 12840069
2005 RanGAP1 is phosphorylated in vivo at Ser-358 by casein kinase II (CK2); this phosphorylation does not alter GAP catalytic activity but stabilizes formation of the ternary RanGAP1·Ran·RanBP1 complex in vivo. MALDI-TOF-MS phosphosite identification, site-directed mutagenesis at S358, in vitro kinase assay with CK2, co-immunoprecipitation of ternary complex Cell structure and function Medium 16428860
2007 RANBP1 depletion in human cells causes prolonged prometaphase, hyperstable spindle microtubules, failure to recruit cyclin B1 to spindles, mislocalization of HURP (DLG7) away from plus-ends, and frequent lagging chromosomes in anaphase, indicating roles in microtubule dynamics regulation and prevention of merotelic attachments. siRNA knockdown in human cells, immunofluorescence microscopy, spindle stability assays Journal of cell science High 17940066
2009 RanBP1 downregulation by RNAi activates caspase-3-dependent apoptosis in multiple transformed cell lines and increases their apoptotic response to taxol; this effect is absent in caspase-3-deficient MCF-7 cells, placing RanBP1 upstream of caspase-3 in the apoptotic pathway. siRNA knockdown, flow cytometry for apoptosis, taxol treatment, caspase-3 activity/deficiency comparison across cell lines Oncogene Medium 19270727
2010 RanBP1 protein abundance peaks in mitosis and must decline in mid-to-late telophase for nuclear reformation. Mild RanBP1 overexpression persisting into late mitosis blocks chromatin decondensation, nuclear expansion, nuclear lamina reorganization, and NPC reassembly, with associated failure of importin-β-dependent NLS cargo reimport. Co-expression of importin-β mitigates these defects. Stable transfection with inducible overexpression, immunofluorescence, quantitative microscopy across cell cycle stages, importin-β co-expression rescue Chromosoma High 20658144
2014 RanBP1 controls RCC1 enzymatic activity and partitioning between chromatin-bound and soluble pools in M-phase Xenopus egg extracts by forming a heterotrimeric RCC1/Ran/RanBP1 complex. This mechanism governs the spatial Ran-GTP gradient that guides spindle assembly. Additionally, phosphorylation of RanBP1 drives changes in chromatin-bound RCC1 dynamics at the metaphase-anaphase transition. Xenopus egg extract biochemistry, chromatin fractionation, in vitro exchange assays, immunodepletion/reconstitution, phosphorylation analysis Developmental cell High 25458009
2014 Loss of Ranbp1 in mice selectively disrupts M phase of the cell cycle in cortical progenitors (both apical at E10.5 and basal at E14.5), resulting in microcephaly and specific reduction of layer 2/3 cortical projection neurons; Ranbp1-/- mice are not recovered live at birth and >60% are exencephalic. Targeted mouse knockout, BrdU/Ki67 proliferation assays, M-phase immunostaining (phospho-H3), cortical layer marker analysis Cerebral cortex High 25452572
2018 RanBP1 regulates cortical neuron axon specification by controlling the cytoplasmic levels of the polarity kinase LKB1/Par4 through the nuclear export machinery; downstream of RanBP1, LKB1 acts via the STK25-GM130 pathway to regulate Golgi organization and promote axonogenesis. shRNA knockdown in cultured cortical neurons and in vivo, LKB1 localization assay, Golgi morphology analysis, epistasis with STK25 and GM130 Cell reports Medium 30184488
2019 In animal cells (in contrast to fungi), RanBP1 dissociates nuclear export complexes by sequestering RanGTP away from the CRM1 export receptor, rather than via RanBP1-CRM1-RanGTP sequestration as in fungi. Animal RanBP1 forms a 1:1:2 (RanBP1:CRM1:RanGTP) nuclear export complex, whereas fungal RanBP1 forms a 1:1:1 complex; this mechanistic divergence is due to loss of affinity between animal RanBP1-RanGTP and CRM1, caused by non-conservation of key residues. In vitro reconstitution of export complexes, stoichiometry determination, mutational analysis of interface residues, biochemical binding assays eLife High 31021318
2020 RanBP1 controls mitotic RCC1 dynamics in human somatic cells: RanBP1 degradation (auxin-inducible degron) alters metaphase chromatin-bound RCC1 exchange rates (FRAP/FLIP) and causes re-localization of the spindle assembly factor HURP, consistent with altered Ran-GTP gradients. Auxin-inducible degron (AID) depletion, FRAP and FLIP of RCC1-GFP, HURP immunolocalization in metaphase cells Cell cycle (Georgetown, Tex.) High 32594833
2022 CD147 interacts with RanBP1 via its intracellular domain (CD147ICD) binding to the C-terminal tail of RanBP1; this interaction mediates CD147-regulated microtubule stability/dynamics and paclitaxel sensitivity in cancer cells. Co-immunoprecipitation, FRET, surface plasmon resonance (SPR), FRAP of microtubule dynamics, truncation analysis, xenograft models Oncogene High 34974521
2022 RanBP1 is required for directional chemotaxis and front-to-rear polarity of migrating neural crest cells during development; this function involves LKB1/PAR4 regulated export downstream of Ran/RanBP1. Morpholino/RNAi knockdown in Xenopus neural crest cells, live cell chemotaxis assays, epistasis with LKB1 Developmental biology Medium 36206829
2023 Oxidative stress concentrates RanBP1 in the nucleus through EGFR and PKA signaling pathways; pharmacological inhibition of EGFR or PKA reduces this relocalization, and mutational analysis identifies Ser-60 and Tyr-103 as critical residues for oxidant-induced nuclear accumulation. Subcellular fractionation, pharmacological inhibitors (EGFR/PKA), site-directed mutagenesis (S60A, Y103F), fluorescence microscopy European journal of cell biology Medium 38011756

Source papers

Stage 0 corpus · 57 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. The EMBO journal 342 7882974
1997 RanBP1 is crucial for the release of RanGTP from importin beta-related nuclear transport factors. FEBS letters 223 9428644
1999 A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export. The Journal of cell biology 181 10330396
1996 RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import. The Journal of cell biology 164 8909533
1996 A nuclear export signal is essential for the cytosolic localization of the Ran binding protein, RanBP1. The Journal of cell biology 135 8794858
1997 Ran-binding protein 1 (RanBP1) forms a ternary complex with Ran and karyopherin beta and reduces Ran GTPase-activating protein (RanGAP) inhibition by karyopherin beta. The Journal of biological chemistry 123 8995296
1995 The C terminus of the nuclear RAN/TC4 GTPase stabilizes the GDP-bound state and mediates interactions with RCC1, RAN-GAP, and HTF9A/RANBP1. The Journal of biological chemistry 109 7782302
1997 Different binding domains for Ran-GTP and Ran-GDP/RanBP1 on nuclear import factor p97. The Journal of biological chemistry 82 9045717
1997 Ran-binding protein 5 (RanBP5) is related to the nuclear transport factor importin-beta but interacts differently with RanBP1. Molecular and cellular biology 75 9271386
2003 Mammalian RanBP1 regulates centrosome cohesion during mitosis. Journal of cell science 73 12840069
2000 Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1. Molecular and cellular biology 70 10779340
1997 Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1. The Journal of biological chemistry 56 9111043
2012 Sgk1 enhances RANBP1 transcript levels and decreases taxol sensitivity in RKO colon carcinoma cells. Oncogene 54 23108393
1997 Dynamic and equilibrium studies on the interaction of Ran with its effector, RanBP1. Biochemistry 54 9315840
2007 RANBP1 localizes a subset of mitotic regulatory factors on spindle microtubules and regulates chromosome segregation in human cells. Journal of cell science 52 17940066
1997 Expression of the murine RanBP1 and Htf9-c genes is regulated from a shared bidirectional promoter during cell cycle progression. The Biochemical journal 49 9224656
2014 Ranbp1, Deleted in DiGeorge/22q11.2 Deletion Syndrome, is a Microcephaly Gene That Selectively Disrupts Layer 2/3 Cortical Projection Neuron Generation. Cerebral cortex (New York, N.Y. : 1991) 46 25452572
1998 EVH1/WH1 domains of VASP and WASP proteins belong to a large family including Ran-binding domains of the RanBP1 family. FEBS letters 42 9883880
2014 RanBP1 governs spindle assembly by defining mitotic Ran-GTP production. Developmental cell 40 25458009
1999 Conformational states of the nuclear GTP-binding protein Ran and its complexes with the exchange factor RCC1 and the effector protein RanBP1. Biochemistry 36 10471274
1997 The balance of RanBP1 and RCC1 is critical for nuclear assembly and nuclear transport. Molecular biology of the cell 36 9348536
2002 Fluorescence resonance energy transfer biosensors that detect Ran conformational changes and a Ran x GDP-importin-beta -RanBP1 complex in vitro and in intact cells. The Journal of biological chemistry 35 12034733
1997 Deregulated expression of the RanBP1 gene alters cell cycle progression in murine fibroblasts. Journal of cell science 34 9410874
1995 RanBP1, a Ras-like nuclear G protein binding to Ran/TC4, inhibits RCC1 via Ran/TC4. Molecular & general genetics : MGG 34 7616957
2009 RanBP1 downregulation sensitizes cancer cells to taxol in a caspase-3-dependent manner. Oncogene 32 19270727
1995 The RCC1 protein interacts with Ran, RanBP1, hsc70, and a 340-kDa protein in Xenopus extracts. The Journal of biological chemistry 31 7738003
2017 SGK1 affects RAN/RANBP1/RANGAP1 via SP1 to play a critical role in pre-miRNA nuclear export: a new route of epigenomic regulation. Scientific reports 30 28358001
1999 Two E2F sites control growth-regulated and cell cycle-regulated transcription of the Htf9-a/RanBP1 gene through functionally distinct mechanisms. The Journal of biological chemistry 26 10187822
1998 A RanBP1 mutation which does not visibly affect nuclear import may reveal additional functions of the ran GTPase system. Experimental cell research 25 9770360
2010 Nuclear reformation after mitosis requires downregulation of the Ran GTPase effector RanBP1 in mammalian cells. Chromosoma 20 20658144
2002 RanBP1, a velocardiofacial/DiGeorge syndrome candidate gene, is expressed at sites of mesenchymal/epithelial induction. Mechanisms of development 20 11804793
2000 Random mutagenesis and functional analysis of the Ran-binding protein, RanBP1. The Journal of biological chemistry 20 10660567
2018 RanBP1 Couples Nuclear Export and Golgi Regulation through LKB1 to Promote Cortical Neuron Polarity. Cell reports 18 30184488
2023 RANBP1 (RAN Binding Protein 1): The Missing Genetic Piece in Cancer Pathophysiology and Other Complex Diseases. Cancers 17 36672435
2021 RANBP1 promotes colorectal cancer progression by regulating pre-miRNA nuclear export via a positive feedback loop with YAP. Oncogene 17 34615998
2020 RanBP1 controls the Ran pathway in mammalian cells through regulation of mitotic RCC1 dynamics. Cell cycle (Georgetown, Tex.) 16 32594833
2005 Phosphorylation of RanGAP1 stabilizes its interaction with Ran and RanBP1. Cell structure and function 16 16428860
2001 A role for the basic patch and the C terminus of RanGTP in regulating the dynamic interactions with importin beta, CRM1 and RanBP1. Journal of molecular biology 16 11124902
2001 Ran binding protein RanBP1 in zebrafish embryonic development. Molecular reproduction and development 16 11424209
2001 XMog1, a nuclear ran-binding protein in Xenopus, is a functional homologue of Schizosaccharomyces pombe mog1p that co-operates with RanBP1 to control generation of Ran-GTP. Journal of cell science 16 11686304
2019 Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals. eLife 15 31021318
1998 Interactions with single-stranded and double-stranded DNA-binding factors and alternative promoter conformation upon transcriptional activation of the Htf9-a/RanBP1 and Htf9-c genes. The Journal of biological chemistry 14 9417108
2022 CD147 supports paclitaxel resistance via interacting with RanBP1. Oncogene 12 34974521
2022 RanBP1 plays an essential role in directed migration of neural crest cells during development. Developmental biology 10 36206829
2023 RanBP1: A Potential Therapeutic Target for Cancer Stem Cells in Lung Cancer and Glioma. International journal of molecular sciences 9 37047826
2019 Nicotiana benthamiana RanBP1-1 Is Involved in the Induction of Disease Resistance via Regulation of Nuclear-Cytoplasmic Transport of Small GTPase Ran. Frontiers in plant science 9 30906303
1999 Isolated mammalian and Schizosaccharomyces pombe ran-binding domains rescue S. pombe sbp1 (RanBP1) genomic mutants. Molecular biology of the cell 8 10397757
2023 RANBP1, a member of the nuclear-cytoplasmic trafficking-regulator complex, is the terminal-striking point of the SGK1-dependent Th17+ pathological differentiation. Frontiers in immunology 7 37441077
2023 Oxidative stress and signaling through EGFR and PKA pathways converge on the nuclear transport factor RanBP1. European journal of cell biology 5 38011756
2025 RANBP1 Regulates NOTCH3-Mediated Autophagy in High Glucose-Induced Vascular Smooth Muscle Cells. Frontiers in bioscience (Landmark edition) 4 40018934
2023 Ranbp1 modulates morphogenesis of the craniofacial midline in mouse models of 22q11.2 deletion syndrome. Human molecular genetics 3 36790128
2001 Molecular cloning of CaYRB1, the Candida albicans RanBP1/YRB1 homologue. Yeast (Chichester, England) 2 11447597
2025 The role of RANBP1 in regulating MiRNA expression and apoptosis in breast cancer cells. Genes & genomics 1 40839321
2024 Unveiling the Movement of RanBP1 During the Cell Cycle and Its Interaction with a Cyclin-Dependent Kinase (CDK) in Plants. International journal of molecular sciences 1 39795905
2026 Serum- and Glucocorticoid-Regulated Kinase 1 Regulates Spindle Assembly Through RanBP1 During Oocyte Meiosis. Journal of cellular physiology 0 42206511
2025 RANBP1 promotes immune evasion in triple-negative breast cancer by suppressing T cell infiltration via the miR-769-5p/PRUNE2 axis. Discover oncology 0 41186818
2024 Quantitative proteomic landscape of the pathophysiology of adhesive arachnoiditis and its clinical significance: Structure and mechanism of TNC and RANBP1 proteins. International journal of biological macromolecules 0 39662555

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