Affinage

SNRNP70

U1 small nuclear ribonucleoprotein 70 kDa · UniProt P08621

Length
437 aa
Mass
51.6 kDa
Annotated
2026-06-10
52 papers in source corpus 27 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SNRNP70/U1-70K is the U1 snRNP-specific RNA-binding protein that nucleates early spliceosome assembly by directly and sequence-specifically contacting loop I of U1 snRNA through its RNP-consensus RNA recognition motif (PMID:2531275). Beyond the core RRM, flanking elements of the extended RRM (an N-terminal helix and a C-terminal disordered region) cooperatively stabilize the U1-70K/stem-loop RNA complex (PMID:35876068). U1-70K bridges U1 snRNP to SR splicing regulators: its RS domain binds SRSF1/ASF (SF2) (PMID:9685421), and at the 5' splice site an RRM-RRM contact between U1-70K and SRSF1, together with a dominant phosphorylated-RS-domain/BAD1-domain interaction, recruits U1 snRNP to enable E-complex formation (PMID:21536904, PMID:39023093). These interactions are governed by phosphorylation switches: CLK1 phosphorylation of Ser-226 releases U1-70K from subnuclear granules and frees the RRM to engage SRSF1, with SRPK1 recycling CLK1 (PMID:33811140), while phosphorylation of the SRSF1 RS domain releases an intramolecular RRM-RS contact to permit intermolecular binding (PMID:21536904). The phosphorylation state of U1-70K itself is critical for catalysis, as dephosphorylation-resistant 70K permits spliceosome assembly but blocks splicing at a pre-catalytic step (PMID:8387646). U1-70K additionally suppresses polyadenylation by directly inhibiting poly(A) polymerase when bound within U1 snRNP (PMID:9659922), and acts with ZFC3H1 to retain 5'-splice-site-containing mRNAs in nuclear speckles, preventing their export (PMID:35351812). It associates RNA-independently with the SMN complex via its N-terminal tail and is required for nuclear gem integrity (PMID:25052091). A cytoplasmic axonal pool regulates local transcript abundance and alternative splicing to control motor axon growth and synaptogenesis (PMID:36384140). Its C-terminal low-complexity domains drive liquid-liquid phase separation that can transition to aggregation (PMID:31723601), and in Alzheimer's disease U1-70K undergoes LC1/LC2-dependent aggregation and proteolytic cleavage to a neurotoxic N40K fragment (PMID:25355317, PMID:24902715).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1987 Medium

    Establishing the molecular identity of U1-70K—an RNA-binding protein of true mass ~52 kDa with multiple alternatively spliced isoforms—provided the foundational reagent and revealed isoform diversity at the gene's outset.

    Evidence cDNA cloning, in vitro RNA-binding, SDS-PAGE and RNA blotting

    PMID:2447561

    Open questions at the time
    • Did not map the RNA-binding interface
    • Functional distinction among isoforms unresolved
  2. 1989 High

    Mapping the RNA target answered how U1-70K is targeted within U1 snRNP, showing its RNP-motif RRM binds loop I of U1 snRNA directly and sequence-specifically.

    Evidence Deletion and point mutagenesis of fusion and native proteins in in vitro RNA-binding assays

    PMID:2531275

    Open questions at the time
    • No structure of the bound complex
    • Contribution of flanking regions not addressed
  3. 1993 High

    Demonstrating that 70K phosphorylation state gates catalysis distinguished spliceosome assembly from activation, placing U1-70K phosphorylation at a pre-catalytic checkpoint.

    Evidence Thiophosphorylation and depletion-reconstitution of U1 snRNPs in HeLa splicing extracts with assembly readouts

    PMID:8387646

    Open questions at the time
    • The responsible kinase not identified at the time
    • Relevant phosphosites unmapped
  4. 1998 High

    Identifying the U1-70K RS domain as the SRSF1-binding module and a substrate of SRPK1 connected U1 snRNP to SR-protein splicing regulators through a phosphorylation-controlled interface.

    Evidence Yeast two-hybrid, far-Western, and mutagenesis (idx4); in vitro PAP inhibition and interaction assays (idx3)

    PMID:9659922 PMID:9685421

    Open questions at the time
    • Structural basis of RS-domain binding unresolved at this stage
    • Connection between PAP inhibition and SR-protein recruitment unclear
  5. 2011 High

    Defining the SRSF1 RRM-U1-70K RRM interaction as a phosphorylation-controlled switch explained mechanistically how SR proteins recruit U1 snRNP to the 5' splice site during E-complex formation.

    Evidence Co-IP, GST pulldown, in vitro E-complex assays, RRM point mutations and phospho-state analysis

    PMID:21536904

    Open questions at the time
    • Did not resolve the dominant RS/BAD1 contact later identified
    • In vivo dynamics of the switch unaddressed
  6. 2021 High

    Identifying CLK1 phosphorylation of Ser-226 as the trigger that releases U1-70K from granules answered how the RRM is freed to engage SRSF1, with SRPK1 recycling the kinase.

    Evidence Phosphoproteomics, Co-IP, subnuclear imaging, in vitro kinase assays, SRPK1 overexpression

    PMID:33811140

    Open questions at the time
    • Upstream signals controlling CLK1 activity unknown
    • Quantitative kinetics of granule release unresolved
  7. 2024 High

    Resolving the phosphorylated-RS/BAD1 interaction as dominant, with RRM-RRM stabilizing, and a conformational switch in BAD1, refined the architecture and regulation of the U1-70K/SRSF1 interface.

    Evidence Circular dichroism, in vitro binding with phosphoproteins, in vitro splicing, in-cell CRISPR saturated domain scanning

    PMID:39023093

    Open questions at the time
    • High-resolution structure of the assembled interface lacking
    • Interplay with U1-70K Ser-226 phosphorylation not fully integrated
  8. 2022 Medium

    Characterizing the extended RRM showed that disordered flanking regions act collectively to stabilize RNA binding, extending the RNA-recognition determinants beyond the canonical RRM.

    Evidence Thermal dissociation, laser T-jump kinetics, all-atom MD simulations, truncation analysis

    PMID:35876068

    Open questions at the time
    • No experimental structure of the eRRM-RNA complex
    • Cellular consequences of flanking-region truncation untested
  9. 2014 Medium

    Mapping the SMN-complex binding to the N-terminal tail and showing a requirement for gem integrity expanded U1-70K's role into snRNP biogenesis bodies.

    Evidence RNA-independent reciprocal Co-IP, deletion mapping, immunofluorescence, knockdown rescue

    PMID:25052091

    Open questions at the time
    • Direct vs. indirect SMN contact not distinguished
    • Mechanism by which U1-70K maintains gem structure unknown
  10. 2022 Medium

    Discovery of a cytoplasmic axonal pool established an extra-nuclear function in local transcript regulation and synaptogenesis, broadening U1-70K beyond nuclear splicing.

    Evidence Live imaging and knockdown in zebrafish with axonal transcript, AChR clustering, and agrin splicing readouts

    PMID:36384140

    Open questions at the time
    • Mechanism of axonal targeting unknown
    • Whether axonal splicing occurs locally or upstream unresolved
  11. 2022 Medium

    Linking U1-70K with ZFC3H1 in nuclear-speckle retention of 5'-splice-site-containing mRNAs defined a quality-control function preventing export of misprocessed transcripts.

    Evidence Fractionation sequencing, reporter retention assays, knockdowns, speckle disassembly

    PMID:35351812

    Open questions at the time
    • Direct vs. indirect U1-70K/ZFC3H1 association not shown
    • How retained transcripts are subsequently resolved unclear
  12. 2014 Medium

    Identifying LC1/LC2-dependent aggregation and proteolytic cleavage to a neurotoxic N40K fragment in Alzheimer's disease connected U1-70K low-complexity domains to neurodegeneration.

    Evidence Sarkosyl fractionation, cross-linking MS, domain deletion (idx14); LC-MS/MS site mapping and primary neuron toxicity assay (idx15)

    PMID:24902715 PMID:25355317

    Open questions at the time
    • Protease responsible for N40K cleavage unidentified
    • Causal role of aggregation in disease pathogenesis not established
  13. 2019 Medium

    Demonstrating that the low-complexity domain undergoes ampholytic-motif-driven phase separation that can convert to aggregation provided a biophysical basis for both speckle/granule partitioning and pathological aggregation.

    Evidence In vitro and in vivo LLPS assays with mutagenesis of basic-acidic motifs

    PMID:31723601

    Open questions at the time
    • Link between LLPS and physiological splicing activity untested
    • Cellular triggers of the LLPS-to-aggregate transition unknown
  14. 2024 Medium

    Showing SNRNP70 directly binds CD55 pre-mRNA and drives osteosarcoma proliferation and metastasis implicated its splicing activity in cancer pathology.

    Evidence RNA-IP, splicing analysis, overexpression/knockdown in vitro and in mouse xenografts

    PMID:39704173

    Open questions at the time
    • Whether CD55 splicing change is sufficient for the phenotype untested
    • Generality across cancer types unknown
  15. 2015 Medium

    Identifying SETMAR-mediated monomethylation of Lys-130 added a non-histone PTM to U1-70K, raising the question of how methylation modulates its function.

    Evidence Quantitative methyl-lysine proteomics, in vitro methylation, cellular MS confirmation

    PMID:25795785

    Open questions at the time
    • Functional consequence of K130 methylation unknown
    • Whether methylation affects RNA binding or splicing untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the multiple regulatory layers—phosphorylation switches, methylation, LLPS, SMN/speckle partitioning, and the cytoplasmic axonal pool—are integrated to control U1-70K function in different cellular contexts remains unresolved.
  • No unified structural model of regulated U1 snRNP/SR-protein assembly
  • Crosstalk between K130 methylation and Ser phosphorylation unexplored
  • Trigger linking physiological LLPS to pathological aggregation in human brain undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 2 GO:0060090 molecular adaptor activity 2 GO:0140110 transcription regulator activity 2 GO:0098772 molecular function regulator activity 1
Localization
GO:0005654 nucleoplasm 2 GO:0005634 nucleus 1 GO:0005829 cytosol 1
Pathway
R-HSA-8953854 Metabolism of RNA 3 R-HSA-74160 Gene expression (Transcription) 2
Partners
CLK1PAPSETMARSMNSRPK1SRSF1ZFC3H1
Complex memberships
SMN complexU1 snRNP

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1989 U1-70K binds directly and sequence-specifically to loop I of U1 snRNA; the central RNP consensus sequence (including the conserved eight-amino-acid RNP motif) is necessary and sufficient for this binding, and at least 8 of the 10 bases in loop I are required. Deletion and mutation analysis of beta-galactosidase/U1-70K fusion proteins and native HeLa U1-70K protein in RNA-binding assays in vitro Molecular and cellular biology High 2531275
1987 Human U1-70K protein binds RNA in vitro; its actual molecular mass is ~52 kDa (not 70 kDa); the gene produces multiple mRNA isoforms via alternative splicing of at least four alternative exon segments, suggesting multiple protein isoforms may exist in vivo. cDNA cloning, in vitro RNA-binding assay, SDS-PAGE, RNA blotting Nucleic acids research Medium 2447561
1993 Phosphorylation state of U1-70K is critical for pre-mRNA splicing: U1 snRNPs bearing thiophosphorylated (dephosphorylation-resistant) 70K fully reconstitute spliceosome assembly but completely block splicing at a pre-catalytic step. An associated kinase activity selectively phosphorylates U1-70K in vitro. In vitro thiophosphorylation with ATP-γS, depletion-reconstitution of U1 snRNPs in HeLa nuclear splicing extracts, spliceosome assembly assays Nature High 8387646
1998 U1-70K, when bound to U1 snRNA within U1 snRNP, directly interacts with and inhibits poly(A) polymerase (PAP), thereby suppressing polyadenylation; U1A within the same snRNP does not contribute to this PAP inhibition. In vitro polyadenylation assays, direct protein-protein interaction assays between U1 70K and PAP Molecular cell High 9659922
1998 The RS domain of U1-70K (residues Arg240–Asp270, containing a repeated Arg-Arg-Arg-Ser-Arg-Ser-Arg-Asp motif) is necessary and sufficient for binding to ASF/SF2 (SRSF1); multiple arginines within this domain are critical for the interaction and are also substrates for phosphorylation by SRPK1. Yeast two-hybrid, far-Western assay, deletion and point mutagenesis of U1-70K The Journal of biological chemistry Medium 9685421
1991 The SR protein SF2/ASF contains an RS domain similar to that of U1-70K, and both contain an RNP-type RNA recognition motif; this structural homology implicates the RS domain of U1-70K as part of a conserved interface linking U1 snRNP to SR splicing regulators. cDNA cloning, recombinant protein expression in bacteria, in vitro splicing assay, sequence comparison Cell Medium 1830244
2011 Early spliceosome (E complex) assembly is mediated by RRM-RRM interaction between SRSF1 and U1-70K; phosphorylation of the SRSF1 RS domain acts as a molecular switch, releasing an intramolecular RRM-RS contact and permitting intermolecular binding to U1-70K RRM, thereby enabling U1 snRNP recruitment to the 5' splice site. Co-immunoprecipitation, GST pulldown, in vitro E-complex formation assay, specific RRM point mutations that disrupt RRM-RRM interaction, phosphorylation-state analysis Proceedings of the National Academy of Sciences of the United States of America High 21536904
2021 CLK1 phosphorylates Ser-226 in the C terminus of U1-70K, releasing U1-70K from subnuclear granules and enabling it to interact with U1 snRNP and SRSF1; this phosphorylation breaks intramolecular contacts between the C terminus and the RRM, freeing the RRM to bind SRSF1. Subsequent nuclear induction of SRPK1 facilitates CLK1 dissociation from U1-70K, recycling the kinase. Quantitative proteomic phosphoproteomics, co-immunoprecipitation, imaging of subnuclear localization, in vitro kinase assays, SRPK1 overexpression Proceedings of the National Academy of Sciences of the United States of America High 33811140
2024 Phosphorylated SRSF1 RS domain interacts with U1-70K BAD1 domain (dominant interaction), while SRSF1 RRM1 interacts with U1-70K RRM (stabilizing interaction); phosphorylation of U1-70K BAD1 inhibits the U1-70K/SRSF1 interaction. BAD1 adopts an α-helical conformation that switches to β-strand/random coil upon RS binding. Circular dichroism, in vitro binding assays with phosphorylated proteins, in vitro splicing assays, in-cell CRISPR-based saturated domain scanning Protein science : a publication of the Protein Society High 39023093
2015 The lysine methyltransferase SETMAR methylates snRNP70 (U1-70K) at lysine 130 in vitro (primarily monomethylation) and in cells, identifying U1-70K as a non-histone substrate of SETMAR. Quantitative proteomic analysis of methylated lysine (mass spectrometry), in vitro methylation assay, cellular confirmation by mass spectrometry The Journal of biological chemistry Medium 25795785
2009 Native mass spectrometry of human U1 snRNP reveals that U1-70K isoforms differentially control subunit dynamics: unstructured, post-translationally modified C-terminal tails of Sm-B/B' and U1-C regulate their dynamic interactions with the Sm core, and these interactions are controlled by binding to different U1-70K isoforms and their phosphorylation status. Native mass spectrometry of intact U1 snRNP complexes; comparison of native vs. recombinant assemblies PloS one Medium 19784376
2008 During early apoptosis, U1-70K undergoes increased phosphorylation at Ser140 (within the RRM), followed by caspase-dependent, PP1-mediated dephosphorylation of other serine residues; the Ser140-phosphorylated form clusters in ectopic RNP-derived structures that are extruded into apoptotic bodies, linking specific phosphorylation events to subcellular redistribution. Immunofluorescence, phospho-specific antibodies, caspase and PP1 inhibitor treatments, cell fractionation, electron microscopy Cell death and differentiation Medium 18202700
2014 U1-70K interacts with the SMN complex in an RNA-independent manner; the SMN complex binding site maps to the unstructured N-terminal tail of U1-70K; U1-70K localizes to nuclear gems and is required for gem integrity. Co-immunoprecipitation (RNA-independent), deletion mapping of U1-70K, immunofluorescence localization, knockdown rescue experiments Journal of cell science Medium 25052091
2019 The low-complexity (LC) domain of U1-70K undergoes liquid-liquid phase separation (LLPS) driven by repetitive basic-acidic (ampholytic) motifs, independent of nucleotides; LLPS can transition to aggregation in vitro and in vivo, with the balance determined by the content of ampholytic motifs. In vitro phase separation assays, in vivo transfection imaging, mutagenesis of basic-acidic motifs Science advances Medium 31723601
2014 AD brain homogenates induce soluble U1-70K to become Sarkosyl-insoluble in a manner dependent on aggregated protein (not RNA); the C-terminal LC1 and LC2 domains of U1-70K are necessary and sufficient for aggregation; a U1-70K fragment harboring the LC1 domain directly interacts with aggregated U1-70K from AD brain. Sarkosyl fractionation, proteinase K treatment, recombinant domain deletion analysis, protein cross-linking and mass spectrometry The Journal of biological chemistry Medium 25355317
2014 U1-70K is proteolytically cleaved in ~50% of Alzheimer's disease cases to an N-terminal ~40 kDa fragment (N40K); the cleavage site maps to a repetitive hydrophilic domain near Arg300 (±6 residues); expression of N40K causes substantial degeneration of rat primary hippocampal neurons. LC-MS/MS with stable isotope labeling, Western blotting with recombinant truncation ladder, primary neuron toxicity assay Journal of proteome research Medium 24902715
2013 U1C protein levels regulate alternative splicing of U1-70K pre-mRNA via a U1C-dependent alternative 3' splice site requiring an adjacent cluster of regulatory 5' splice sites and intact U1 snRNP binding; the non-productive isoform is degraded by NMD, reducing U1-70K mRNA/protein levels and impairing U1C incorporation, establishing a feedback loop controlling U1-70K/U1C homeostasis and U1 snRNP assembly. RNA-Seq after U1C knockdown, minigene mutational analysis, antisense morpholino splice-site blocking, in vitro binding experiments PLoS genetics High 24146627
2001 Yeast U1-70K homolog Snp1p physically associates with the essential spliceosomal protein Prp8p (by co-immunoprecipitation), suggesting Snp1p has functions late in spliceosome development; Snp1p also interacts with Exo84p, a subunit of the exocyst secretion complex, which is itself required for pre-mRNA splicing and prespliceosome formation in vitro. Yeast two-hybrid, co-immunoprecipitation, in vitro splicing assay with temperature-sensitive exo84 mutant, RT-PCR pre-mRNA quantification The Journal of biological chemistry Medium 11425851
1995 In yeast, the N-terminal domain of Snp1 (yeast U1-70K) is necessary and sufficient for complementation of snp1-null growth and splicing defects and for in vivo association with the U1 snRNP particle; the conserved RRM and glycine-rich domains are dispensable for these functions. Yeast genetic complementation with deletion alleles of SNP1, in vivo U1 snRNP association assay Molecular and cellular biology Medium 7565787
1992 The yeast SNP1 gene product binds directly and specifically to the first 47 nucleotides of yeast U1 RNA (including stem-loop I), establishing Snp1p as the yeast ortholog of mammalian U1-70K. Bacterial expression of SNP1 fusion protein, gel-shift RNA-binding assay Nucleic acids research Medium 1387202
2005 The PSI protein (P-element somatic inhibitor) binds U1-70K via two homologous 'A and B box' sequences near its C terminus that interact with a short proline-rich sequence at the C terminus of U1-70K; NMR shows the B box forms an anti-parallel helical hairpin with a hydrophobic cluster of four aromatic residues that contacts the proline-rich region of U1-70K. NMR structure determination, deletion mapping of both PSI and U1-70K interaction domains Journal of molecular biology High 15990112
2004 In Drosophila, loss of U1-70K causes embryonic lethality; the arginine-rich RS domain of U1-70K is dispensable for viability and splicing in otherwise wild-type animals, but becomes essential for viability when combined with mutations in another U1 snRNP component, demonstrating a redundant but context-dependent role for the RS domain in U1 snRNP function. Drosophila genetics: null alleles, RS-domain deletion transgenic rescue, double-mutant analysis Genetics Medium 15611175
2022 U1-70K/SNRNP70 localizes to RNA-associated granules in zebrafish axons (cytoplasmic pool); this extra-nuclear SNRNP70 regulates motor axon growth, acetylcholine receptor clustering, and neuromuscular synaptogenesis; it protects a subset of axonal transcripts and regulates splice variants of agrin to control synapse formation. Live imaging in zebrafish, knockdown experiments, transcript abundance and trafficking measurements in axons, splicing analysis of agrin Current biology : CB Medium 36384140
2022 U1-70K and ZFC3H1 function in the same pathway to retain mRNAs containing 5' splice site motifs (e.g., IPA transcripts) in nuclear speckles, preventing their nuclear export; disassembly of nuclear speckles impairs this nuclear retention. High-throughput sequencing of cellular fractions, reporter mRNA nuclear retention assays, knockdown of U1-70K and ZFC3H1, nuclear speckle disassembly RNA (New York, N.Y.) Medium 35351812
2022 The extended RRM (eRRM) of U1-70K, including the N-terminal flanking helix (N-helix) and C-terminal intrinsically disordered region (C-IDR), is required for full stability of the U1-70K/SL1 RNA complex; the N-helix strongly contributes to overall binding, while the C-IDR affects the local binding site; all-atom simulations show flanking regions act via collective interactions with RNA rather than through direct hydrogen bond contributions. Thermal dissociation assays, laser temperature-jump kinetics, long-time all-atom molecular dynamics simulations, truncation analysis Nucleic acids research Medium 35876068
2024 SNRNP70 directly interacts with CD55 pre-mRNA and modulates its alternative splicing; SNRNP70 overexpression promotes OS cell proliferation and metastasis in vitro, while its depletion reduces these capabilities in vivo. RNA immunoprecipitation (direct interaction with CD55), splicing analysis, overexpression and knockdown functional assays in vitro and in vivo (mouse xenograft) JCI insight Medium 39704173
2025 In yeast, U1 snRNP interaction with RNA polymerase II is mediated predominantly by Prp40 rather than U1-70K (Snp1); residues on yeast U1-70K involved in pol II interaction in humans are not conserved in yeast, and U1-70K makes minimal contribution to U1 snRNP's association with pol II in yeast. Co-immunoprecipitation of pol II with U1/U2 snRNPs, domain deletion analysis of Prp40, comparison with human U1 snRNP cryo-EM data bioRxivpreprint Low 40909591

Source papers

Stage 0 corpus · 52 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1991 Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators. Cell 477 1830244
1998 U1 snRNP inhibits pre-mRNA polyadenylation through a direct interaction between U1 70K and poly(A) polymerase. Molecular cell 250 9659922
2011 Interaction between the RNA binding domains of Ser-Arg splicing factor 1 and U1-70K snRNP protein determines early spliceosome assembly. Proceedings of the National Academy of Sciences of the United States of America 184 21536904
1987 The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding. Nucleic acids research 149 2447561
1993 Thiophosphorylation of U1-70K protein inhibits pre-mRNA splicing. Nature 137 8387646
1989 Direct, sequence-specific binding of the human U1-70K ribonucleoprotein antigen protein to loop I of U1 small nuclear RNA. Molecular and cellular biology 82 2531275
2019 Low-complexity domain of U1-70K modulates phase separation and aggregation through distinctive basic-acidic motifs. Science advances 59 31723601
1988 Structure and expression of a Xenopus gene encoding an snRNP protein (U1 70K). The EMBO journal 57 2468488
2014 Aggregation properties of the small nuclear ribonucleoprotein U1-70K in Alzheimer disease. The Journal of biological chemistry 50 25355317
1998 A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding. The Journal of biological chemistry 48 9685421
2009 Isoforms of U1-70k control subunit dynamics in the human spliceosomal U1 snRNP. PloS one 44 19784376
2005 Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease. Arthritis research & therapy 44 15743477
2000 Characterization of SNP1, a cell wall-degrading trypsin, produced during infection by Stagonospora nodorum. Molecular plant-microbe interactions : MPMI 39 10796020
2014 Integrated approaches for analyzing U1-70K cleavage in Alzheimer's disease. Journal of proteome research 38 24902715
2008 Apoptosis-linked changes in the phosphorylation status and subcellular localization of the spliceosomal autoantigen U1-70K. Cell death and differentiation 38 18202700
2020 Phylogenetic comparison of 5' splice site determination in central spliceosomal proteins of the U1-70K gene family, in response to developmental cues and stress conditions. The Plant journal : for cell and molecular biology 31 32133712
2015 A Proteomic Strategy Identifies Lysine Methylation of Splicing Factor snRNP70 by the SETMAR Enzyme. The Journal of biological chemistry 30 25795785
2013 A novel intra-U1 snRNP cross-regulation mechanism: alternative splicing switch links U1C and U1-70K expression. PLoS genetics 28 24146627
2001 New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. The Journal of biological chemistry 25 11425851
2022 ZFC3H1 and U1-70K promote the nuclear retention of mRNAs with 5' splice site motifs within nuclear speckles. RNA (New York, N.Y.) 23 35351812
1995 The amino-terminal domain of yeast U1-70K is necessary and sufficient for function. Molecular and cellular biology 23 7565787
2021 CLK1 reorganizes the splicing factor U1-70K for early spliceosomal protein assembly. Proceedings of the National Academy of Sciences of the United States of America 22 33811140
1995 Members of a family of proteins (the RD family) detected by a U1 70K monoclonal antibody are present in spliceosomal complexes. Nucleic acids research 22 7479068
2022 Cytoplasmic pool of U1 spliceosome protein SNRNP70 shapes the axonal transcriptome and regulates motor connectivity. Current biology : CB 20 36384140
2004 Intramolecular T cell spreading in unprimed MRL/lpr mice: importance of the U1-70k protein sequence 131-151. Arthritis and rheumatism 20 15476231
2004 The Drosophila U1-70K protein is required for viability, but its arginine-rich domain is dispensable. Genetics 20 15611175
1999 Key residues revealed in a major conformational epitope of the U1-70K protein. Proceedings of the National Academy of Sciences of the United States of America 20 10588732
1992 The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene. Nucleic acids research 20 1387202
2003 Deletion of the SNP1 trypsin protease from Stagonospora nodorum reveals another major protease expressed during infection. Fungal genetics and biology : FG & B 19 12553935
2014 The splicing factor U1-70K interacts with the SMN complex and is required for nuclear gem integrity. Journal of cell science 18 25052091
2015 Structure-function analysis and genetic interactions of the Yhc1, SmD3, SmB, and Snp1 subunits of yeast U1 snRNP and genetic interactions of SmD3 with U2 snRNP subunit Lea1. RNA (New York, N.Y.) 17 25897024
1990 Human U1-70K ribonucleoprotein antigen gene: organization, nucleotide sequence, and mapping to locus 19q13.3. Genomics 15 2147422
2005 Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase. Journal of molecular biology 14 15990112
2000 Characterization of recombinant human autoantibody fragments directed toward the autoantigenic U1-70K protein. European journal of immunology 14 11069087
1997 Autoepitope-mapping of the U1-70K protein with human-Drosophila chimeric proteins. Journal of autoimmunity 12 9451595
2014 Genome-wide RNA-binding analysis of the trypanosome U1 snRNP proteins U1C and U1-70K reveals cis/trans-spliceosomal network. Nucleic acids research 11 24748659
2022 Dramatic response to entrectinib in a patient with malignant peripheral nerve sheath tumor harboring novel SNRNP70-NTRK3 fusion gene. Genes, chromosomes & cancer 10 35906852
2015 Mapping epitopes of U1-70K autoantibodies at single-amino acid resolution. Autoimmunity 9 26333287
1999 Inhibitor peptide SNP-1 binds to a soluble form of BST-1/CD157 at a 2:2 stoichiometry. European journal of biochemistry 9 10491089
2015 Two Routes to Genetic Suppression of RNA Trimethylguanosine Cap Deficiency via C-Terminal Truncation of U1 snRNP Subunit Snp1 or Overexpression of RNA Polymerase Subunit Rpo26. G3 (Bethesda, Md.) 8 25911228
2024 The U1-70K and SRSF1 interaction is modulated by phosphorylation during the early stages of spliceosome assembly. Protein science : a publication of the Protein Society 7 39023093
2022 A specific gene-splicing alteration in the SNRNP70 gene as a hallmark of an ALS subtype. Gene 7 35101583
2021 Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family. Scientific reports 6 34140531
2022 Spliceosomal SL1 RNA binding to U1-70K: the role of the extended RRM. Nucleic acids research 4 35876068
2024 SNRNP70 regulates the splicing of CD55 to promote osteosarcoma progression. JCI insight 3 39704173
2025 U1-70K and U1A and tau pathogenesis in demented and non-demented individuals with Down syndrome. Alzheimer's & dementia : the journal of the Alzheimer's Association 2 40762109
2022 The regulation of lipid and carbohydrate storage by the splicing factor gene snRNP-U1-70K in the Drosophila fat body. microPublication biology 2 35655607
2019 Is the T-G-CT-G SNRNP70 haplotype another proof that mixed connective tissue disease is distinct from systemic lupus erythematosus and systemic sclerosis? A novel gene variant in SNRNP70 gene. Clinical and experimental rheumatology 2 31573470
1995 The RRM domain is dispensable for yeast U1-70K function. Nucleic acids symposium series 1 8643384
2026 Novel activating SNRNP70-ALK fusion in congenital infant-type hemispheric glioma displays clinical response to lorlatinib: a case-report. NPJ precision oncology 0 41748687
2025 U1 snRNP and RNA polymerase II interaction is predominantly mediated by Prp40 rather than U1-70K in yeast. bioRxiv : the preprint server for biology 0 40909591
2024 Expression and Purification of Human U1-70K (snRNP70) and its BAD Domains Using an E. coli Expression System. Current protocols 0 38896106

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