Affinage

SRSF1

Serine/arginine-rich splicing factor 1 · UniProt Q07955

Length
248 aa
Mass
27.7 kDa
Annotated
2026-04-28
100 papers in source corpus 52 papers cited in narrative 52 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SRSF1 is a multifunctional SR protein that couples pre-mRNA splicing with mRNA export, translation, nonsense-mediated decay, and genome stability maintenance. Its two RRMs bind exonic splicing enhancers (RRM1 preferring CN motifs) and U1 snRNA stem-loop 3 to recruit U1 snRNP and U2AF65 for splice-site recognition, while the RS domain mediates phosphorylation-dependent protein–protein interactions essential for spliceosome assembly; sequential SRPK1-directed C-to-N-terminal phosphorylation governs nuclear import and splicing competence, whereas PP1/PP2A-mediated dephosphorylation and PRMT1-catalyzed arginine methylation control cytoplasmic shuttling, TAP/NXF1-TREX recruitment for mRNA export, and cap-dependent translation via mTORC1/4E-BP regulation (PMID:8223480, PMID:9404896, PMID:18155240, PMID:15184380, PMID:37938975, PMID:18439897). SRSF1 prevents co-transcriptional R-loop accumulation to safeguard genomic integrity—demonstrated by R-loop-dependent DNA damage upon its depletion—and interacts with FANCD2 to couple mRNA export with the Fanconi anemia pathway (PMID:16096057, PMID:38165804). As a direct MYC transcriptional target, SRSF1 functions as a proto-oncogene by producing oncogenic splice isoforms of BIN1, MNK2, S6K1, Ron, and MYO1B, activating mTORC1 to drive cellular transformation that is reversible by rapamycin (PMID:17310252, PMID:18832178, PMID:22545246).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1993 High

    Establishing the domain architecture of SRSF1 resolved how a single SR protein contributes to both constitutive and alternative splicing: two synergistic RRMs provide RNA binding specificity while the RS domain is essential for constitutive but dispensable for alternative splicing.

    Evidence In vitro splicing reconstitution with systematic domain deletion and point mutagenesis of RRM and RS domains

    PMID:8223480 PMID:8223481

    Open questions at the time
    • Structural basis for RRM1 vs RRM2 RNA specificity unknown at this stage
    • How the RS domain mechanistically promotes constitutive splicing not resolved
  2. 1995 High

    Demonstrating that SRSF1 forms a ternary complex with U1 snRNP and pre-mRNA at 5′ splice sites established the early spliceosome assembly mechanism: SRSF1 bridges exonic sequences to the splicing machinery.

    Evidence Reconstituted ternary complex assembly with purified U1 snRNP; UV crosslinking and gel-shift binding assays with 5′ splice-site mutants

    PMID:7512732 PMID:7667103 PMID:8253084

    Open questions at the time
    • Whether SRSF1 contacts U1 snRNA directly or only via U1-C protein was unresolved
    • Mechanism of U2AF65 recruitment by SRSF1 not yet addressed
  3. 1998 High

    The finding that both phosphorylation (for spliceosome assembly) and dephosphorylation (for catalysis) of the RS domain are required resolved why splicing is a phosphorylation-cycle-dependent process and identified SRPK1/2 as cognate kinases controlling nuclear localization.

    Evidence Thiophosphorylated SRSF1 blocks catalytic step in vitro; SRPK1/2 binding and kinase assays; dominant-negative SRPK2 causes cytoplasmic retention

    PMID:10196197 PMID:9404896 PMID:9799243

    Open questions at the time
    • Identity of the phosphatase(s) catalyzing splicing-coupled dephosphorylation unknown
    • How many RS-domain serines must cycle was not mapped
  4. 2001 High

    Showing that SRSF1 recruits U2AF65 to weak polypyrimidine tracts via exonic splicing enhancers—even without its RS domain—revealed a second, RS-independent mechanism for splice-site activation and a dual role for RRM2 in both activation and position-dependent repression.

    Evidence In vitro splicing and U2AF65 binding assays with RS-deleted SRSF1; MS2-tethering assays identifying SWQDLKD motif in RRM2 as repressor determinant

    PMID:11575921 PMID:11801589

    Open questions at the time
    • Structural basis for the SWQDLKD-mediated repression not determined
    • Whether RS-independent recruitment operates on endogenous targets in vivo unknown
  5. 2005 High

    The discovery that SRSF1 depletion causes R-loop-dependent DNA damage established a genome-stability function independent of splicing regulation, showing that co-transcriptional mRNP packaging by SRSF1 prevents RNA:DNA hybrid accumulation.

    Evidence siRNA depletion with comet assay and R-loop detection; in vitro transcription reconstitution; RNase H overexpression rescue

    PMID:16096057

    Open questions at the time
    • Whether R-loop prevention requires specific RNA binding or generic mRNP coating was unclear
    • Which genomic loci are most vulnerable to SRSF1-depletion-induced R-loops not mapped
  6. 2005 High

    Identification of oncogenic splice targets (Ron/ΔRon, BIN1, MNK2, S6K1) and demonstration that SRSF1 overexpression transforms cells established SRSF1 as a proto-oncogene acting through alternative splicing of tumor-relevant transcripts.

    Evidence Overexpression transformation and nude mouse xenografts; minigene splicing analysis; shRNA rescue of transformation by S6K1 isoform-2 knockdown

    PMID:16364913 PMID:17310252

    Open questions at the time
    • Full catalogue of oncogenic splice targets not determined
    • Whether transformation requires all targets simultaneously or single events suffice unclear
  7. 2007 High

    Elucidation of SRPK1's directional, processive C-to-N phosphorylation mechanism on the RS1 segment explained how graded phosphorylation generates functionally distinct SRSF1 phospho-states.

    Evidence Engineered protease footprinting and single-turnover kinetics with systematic Arg-to-Lys and Ser-to-Ala mutations

    PMID:18155240 PMID:18687337 PMID:19477182

    Open questions at the time
    • How CLK-family kinases cooperate with SRPKs to complete RS2 phosphorylation not addressed
    • Structural basis of the initiation box not visualized
  8. 2008 High

    Demonstrating that SRSF1 activates mTORC1 and promotes cap-dependent translation via 4E-BP suppression linked its cytoplasmic functions to signaling and explained the non-splicing component of its oncogenic activity.

    Evidence Co-IP of SRSF1 with mTOR and PP2A; rapamycin reversal of transformation in vitro and in vivo; 4E-BP phosphorylation assays

    PMID:18439897 PMID:18832178

    Open questions at the time
    • Direct mechanism by which SRSF1 activates mTORC1 (kinase vs adaptor) not resolved
    • Whether translational targets overlap with splicing targets unclear
  9. 2010 High

    Multiple studies converged to reveal a homeostatic autoregulatory network: SRSF1 represses its own translation via RRM2 and its ultraconserved 3′UTR, promotes pri-miR-7 processing that feeds back to suppress SRSF1, and is regulated by Sam68-mediated AS-NMD and PRMT-dependent arginine methylation controlling nucleo-cytoplasmic distribution.

    Evidence 3′UTR reporter and polyribosome assays; Drosha cleavage reconstitution; Sam68 OE/KD with splice-variant RT-PCR; methylation-site mutagenesis with localization imaging

    PMID:20139984 PMID:20308322 PMID:20385090 PMID:20876280

    Open questions at the time
    • Quantitative contribution of each autoregulatory arm to steady-state SRSF1 levels unknown
    • Whether miR-7 feedback operates in all tissues not established
  10. 2012 High

    Identification of MYC as a direct transcriptional activator of SRSF1 established a MYC→SRSF1→oncogenic splicing axis and showed that SRSF1 cooperates with MYC to transform mammary epithelial cells via eIF4E potentiation.

    Evidence ChIP of MYC at SRSF1 promoter E-boxes; 3D mammary acinar culture and orthotopic transplantation; RRM domain requirement mapping

    PMID:22245967 PMID:22545246

    Open questions at the time
    • Whether MYC-SRSF1 axis is universally oncogenic or tissue-specific not resolved
    • Contribution of translational vs splicing functions in mammary transformation not separated
  11. 2018 High

    Demonstrating that SRSF1 enhances NMD by recruiting UPF1 downstream of premature termination codons, bypassing UPF2, unified SRSF1's splicing and mRNA surveillance functions and showed parallels to EJC-dependent NMD.

    Evidence UV-crosslinking RNA-IP for UPF1 binding; tethered-function NMD reporter assays with UPF2-independence demonstration

    PMID:29768215

    Open questions at the time
    • Whether SRSF1-dependent NMD operates on its autoregulatory transcripts in the same manner unknown
    • Structural basis of SRSF1-UPF1 interaction not determined
  12. 2018 High

    NMR identification of the PP1-binding surface on RRM1 and its role in RS domain dephosphorylation and speckle dynamics established how phosphatase access is structurally encoded, complementing prior kinase studies.

    Evidence NMR spectroscopy of RRM1–PP1 interface; mutagenesis of binding residues with phosphorylation state and speckle dynamics analysis

    PMID:30185622

    Open questions at the time
    • Whether PP2A uses the same or different binding surface not addressed
    • In vivo contribution of PP1 vs PP2A to splicing-coupled dephosphorylation not distinguished
  13. 2021 High

    Structural determination of the RRM1–RNA complex (CN motif preference) and demonstration that SRSF1 is recruited to exons independently via U1 snRNA stem-loop 3 contact resolved the bimodal RNA-recognition mechanism underlying exon definition.

    Evidence NMR solution structure of RRM1–RNA; E87N gain-of-function mutagenesis for SMN exon 7; single-molecule co-localization assays with U1 snRNA SL3 mutations

    PMID:33462199 PMID:34779515

    Open questions at the time
    • Full structural model of SRSF1 simultaneously engaging ESE and U1 snRNA not available
    • Whether SL3-mediated recruitment occurs for all SRSF1-dependent exons unknown
  14. 2021 High

    Tissue-specific conditional knockouts revealed essential in vivo roles: SRSF1 cytoplasmic shuttling drives multiciliogenesis-related mRNA translation, SRSF1 maintains hepatocyte genome stability by preventing R-loops, and SRSF1 is required for neuromuscular junction maturation.

    Evidence NRS knock-in mouse with ciliary defects and polyribosome profiling; liver-specific KO with R-loop detection, eCLIP, and NASH-like pathology; myogenic progenitor KO with NMJ electrophysiology

    PMID:32888503 PMID:34338635 PMID:36759613

    Open questions at the time
    • Whether translational and splicing functions can be genetically separated in vivo not tested
    • Cell-type-specific target repertoires not systematically compared
  15. 2024 High

    Discovery that SRSF1 physically interacts with FANCD2 to stimulate its monoubiquitination, which in turn promotes SRSF1–NXF1 export complex assembly, linked mRNA export to the Fanconi anemia DNA repair pathway and explained how cancer-associated SRSF1 mutations cause R-loop accumulation.

    Evidence Reciprocal co-IP of SRSF1–FANCD2; monoubiquitination assays; mRNA export measurements; R-loop detection with cancer-associated SRSF1 mutants

    PMID:38165804

    Open questions at the time
    • Whether FANCD2 monoubiquitination by SRSF1 occurs on chromatin or in soluble nucleoplasm not determined
    • Structural basis of SRSF1–FANCD2 interaction unknown
    • Whether other SR proteins similarly engage the FA pathway untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • An integrated structural model showing how phosphorylation, methylation, and RNA binding cooperatively regulate SRSF1 transitions between splicing, export, translation, and genome-stability functions—and how these are deregulated in specific cancers—remains to be constructed.
  • No full-length SRSF1 structure in complex with physiological RNA and protein partners
  • Quantitative models relating phospho-state distribution to functional output lacking
  • Systematic identification of cancer-type-specific pathogenic splice targets incomplete

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 6 GO:0098772 molecular function regulator activity 4 GO:0060090 molecular adaptor activity 3
Localization
GO:0005634 nucleus 4 GO:0005654 nucleoplasm 4 GO:0005829 cytosol 4
Pathway
R-HSA-8953854 Metabolism of RNA 7 R-HSA-162582 Signal Transduction 4 R-HSA-1643685 Disease 4 R-HSA-73894 DNA Repair 4 R-HSA-392499 Metabolism of proteins 3 R-HSA-74160 Gene expression (Transcription) 2 R-HSA-9612973 Autophagy 1
Partners
FANCD2MYCNXF1PPP1CAPRMT1SRPK1U2AF2UPF1
Complex memberships
TREX mRNA export complexU1 snRNP (ternary complex with pre-mRNA)mTORC1

Evidence

Reading pass · 52 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 SF2/ASF has two functional domains: an N-terminal RNA recognition motif (RRM) with specific protein-RNA contacts (Phe56 and Phe58 in RNP-1 submotif essential for constitutive splicing) and a C-terminal RS domain required for constitutive splicing; the two RRMs act synergistically for RNA binding, while alternative splicing activity does not require the RS domain. In vitro splicing assays with site-directed mutagenesis of RRM and RS domain; RNA binding assays with recombinant mutant proteins The EMBO journal High 8223480
1993 ASF/SF2 contains two diverged RBDs both essential for constitutive splicing activity; the RS domain is required for essential splicing but not for splice site switching; deletion isoforms lacking either domain act as dominant-negative splicing repressors. In vitro splicing assays with deletion and substitution mutants of ASF/SF2 The EMBO journal High 8223481
1993 SF2/ASF enhances downstream 5' splice site use by increasing the strength of U1 snRNP binding indiscriminately to all sites, as shown by ribonuclease H protection experiments. Ribonuclease H protection assay; U1 snRNP cleavage and site mutation experiments The EMBO journal High 8253084
1994 ASF/SF2 can specifically recognize pre-mRNA 5' splice sites in a sequence-specific manner; point mutations in the 5' splice site consensus reduce binding up to 100-fold. UV crosslinking and gel shift assays with purified recombinant ASF/SF2 and mutant pre-mRNAs Proceedings of the National Academy of Sciences of the United States of America High 7512732
1995 ASF/SF2 forms a ternary complex with U1 snRNP and pre-mRNA dependent on a functional 5' splice site; complex formation requires one functional RNA binding domain and the RS domain of ASF/SF2, and the U1-C protein of U1 snRNP. Reconstituted ternary complex assembly with purified components; domain deletion analysis Nucleic acids research High 7667103
1997 Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro: phosphorylated ASF/SF2 promotes spliceosome assembly, while dephosphorylation is required for the first transesterification reaction; thiophosphorylated ASF/SF2 (resistant to dephosphorylation) blocks the first catalytic step. In vitro splicing assays with phosphorylated, unphosphorylated, and thiophosphorylated ASF/SF2 in SR-depleted HeLa extracts RNA (New York, N.Y.) High 9404896
1998 Phosphorylation of the RS domain of ASF/SF2 differentially affects its protein-protein interactions: it positively affects interaction with U1 snRNP 70K, and differentially (positively, negatively, or not at all) regulates interactions with other splicing factors; phosphorylation is required for constitutive splicing but not for splicing activation. Co-immunoprecipitation and interaction assays comparing phosphorylated vs. unphosphorylated ASF/SF2 The EMBO journal High 9799243
1998 cdc2 kinase (cdc2-cyclin B complex) phosphorylates the RS domain of SF2/ASF specifically at serines 227, 238, and 199, suggesting cell-cycle-dependent regulation of splicing. In vitro kinase assay with recombinant cdc2 kinase and mutant SF2/ASF proteins; phosphopeptide mapping Biochemical and biophysical research communications Medium 9731229
1999 SRPK1 and SRPK2 physically bind to and phosphorylate SF2/ASF; binding maps to an (Arg-Ser)8 repeat region beginning at residue 204; SRPKs bind unphosphorylated but not phosphorylated SF2/ASF; expression of kinase-inactive SRPK2 causes cytoplasmic accumulation of SF2/ASF, establishing that SRPK-mediated phosphorylation regulates SF2/ASF nuclear localization. GST-SF2/ASF affinity chromatography from HeLa cells; recombinant SRPK1/2 in vitro kinase and binding assays; co-IP in COS7 cells; far-Western analysis; dominant-negative SRPK2 expression with localization imaging The Journal of biological chemistry High 10196197
2001 SF2/ASF binds to an exonic splicing enhancer (ESE3) in HIV-1 tat exon and stimulates U2AF65 binding to a suboptimal polypyrimidine tract in an ESE3-dependent manner; this activity does not require the RS domain, demonstrating an RS-domain-independent mechanism for U2AF recruitment. In vitro splicing assays; U2AF65 binding assay; deletion mutants of SF2/ASF lacking RS domain Journal of molecular biology High 11575921
2002 The second RNA binding domain (RBD2) of ASF/SF2 is both necessary and sufficient for splicing repressor function; the SWQDLKD motif within RBD2 is essential for splicing repression but not for RNA binding; repressor activity is position-dependent (intronic but not exonic). MS2-ASF/SF2 fusion protein tethering assay; domain deletion and substitution mutagenesis; in vitro splicing The Journal of biological chemistry High 11801589
2004 Hypophosphorylated ASF/SF2 preferentially binds the mRNA export receptor TAP; overexpression of ASF/SF2 relocalizes TAP to nuclear speckles; ASF/SF2 in mature mRNP is hypophosphorylated while nucleoplasmic ASF/SF2 is hyperphosphorylated, indicating dephosphorylation promotes incorporation into mRNPs and TAP recruitment for mRNA export. Immunoprecipitation with phospho-specific conditions; subnuclear fractionation; nuclear speckle co-localization imaging The Journal of biological chemistry High 15184380
2004 SF2/ASF recruitment to nuclear stress bodies (nSBs) upon heat shock is mediated by its second RNA recognition motif (RRM2); mutations in the first alpha-helix of RRM2 abrogate association with nSBs and disrupt alternative splicing activity in vivo; SF2/ASF binds satellite III transcripts via RRM2. Mutagenesis of RRM2 with immunofluorescence localization; in vivo alternative splicing assay; gel mobility shift assay with RRM2 domain Nucleic acids research High 15302913
2005 Depletion of ASF/SF2 in vivo causes DNA double-strand breaks and R-loop formation (RNA:DNA hybrids) on the non-template strand of transcribed genes; ASF/SF2 prevents R-loop formation in a reconstituted in vitro transcription system; stable RNase H overexpression suppresses the DNA fragmentation and hypermutation phenotypes. siRNA depletion with comet assay and DNA fragmentation analysis; in vitro transcription reconstitution; RNase H overexpression rescue Cell High 16096057
2005 SF2/ASF controls alternative splicing of Ron proto-oncogene by binding a purine-rich exonic splicing enhancer in exon 12, promoting skipping of exon 11 to produce the constitutively active DeltaRon isoform; this drives epithelial-to-mesenchymal transition and cell motility. Overexpression and RNAi experiments; minigene splicing assays; cell motility assays; DeltaRon-specific knockdown rescue Molecular cell High 16364913
2007 SF2/ASF is a proto-oncogene that transforms immortal rodent fibroblasts; it controls alternative splicing of BIN1 (removing tumor suppressor activity), MNK2 (producing a MAP kinase-independent eIF4E kinase isoform), and S6K1 (producing an oncogenic isoform); knockdown of either SF2/ASF or S6K1 isoform-2 reverses transformation. Overexpression transformation assays; nude mouse xenograft; alternative splicing minigene analysis; shRNA knockdown Nature structural & molecular biology High 17310252
2007 SRPK1 phosphorylates ASF/SF2 RS domain in a directional, processive manner from C-terminus to N-terminus (RS1 region), using a 'grab-and-pull' mechanism; initiation requires a docking groove near the C-terminus of RS1 and proceeds N-terminally. Region-specific phosphorylation monitoring via engineered protease cleavage sites; Arg-to-Lys and Ser-to-Ala mutations; in vitro kinase assays with single-turnover analysis Journal of molecular biology High 18155240
2008 SF2/ASF promotes translation initiation of bound mRNAs by suppressing 4E-BP activity through interactions with both mTOR and phosphatase PP2A, functioning as an adaptor to regulate cap-dependent translation of specific mRNAs; this requires eIF4E. In vivo translation assays; co-immunoprecipitation of SF2/ASF with mTOR and PP2A; 4E-BP phosphorylation assays Molecular cell High 18439897
2008 SF2/ASF activates the mTORC1 branch of the mTOR signaling pathway (measured by S6K and eIF4EBP1 phosphorylation), bypassing upstream PI3K/Akt signaling; mTORC1 activation is essential for SF2/ASF-mediated transformation, as rapamycin blocks transformation in vitro and in vivo. S6K/eIF4EBP1 phosphorylation assays; rapamycin treatment; shRNA knockdown of mTOR, Raptor, Rictor; in vitro transformation and nude mouse tumor assays Proceedings of the National Academy of Sciences of the United States of America High 18832178
2008 SRPK1 uses adaptable molecular interactions including an electropositive pocket stabilizing newly phosphorylated serines to facilitate processive phosphorylation of ASF/SF2; the RRM1-RRM2 module fine-tunes processivity during later phases of phosphorylation. Single-turnover and multiturnover kinetic methods; deletion studies; mutational analysis of SRPK1-SF2/ASF interface Journal of molecular biology High 18687337
2009 SRPK1 initiates phosphorylation of ASF/SF2 in an 'initiation box' at the C-terminal end of the RS1 segment (middle of RS domain) and proceeds N-terminally; this requires both a docking groove in SRPK1's large lobe and RRM2 on the N-terminal flank of the RS domain. Engineered footprinting; single-turnover kinetic experiments; Arg-to-Lys and deletion mutations Journal of molecular biology High 19477182
2010 SF2/ASF negatively autoregulates its own expression through multiple post-transcriptional mechanisms: unproductive alternative splicing generating NMD-targeted isoforms, and primarily translational repression via the ultraconserved 3'UTR mediated by RRM2; overexpression shifts target mRNA to monoribosomes. Alternative splicing isoform characterization; reporter assays with 3'UTR constructs; polyribosome fractionation; Dicer-independent translation repression analysis Nature structural & molecular biology High 20139984
2010 Sam68 promotes AS-NMD (alternative splicing coupled nonsense-mediated decay) of SF2/ASF transcripts, reducing SF2/ASF protein levels during EMT; Sam68 phosphorylation by ERK1/2 signaling controls this regulation. Overexpression and siRNA knockdown of Sam68; RT-PCR analysis of SF2/ASF splice variants; ERK1/2 inhibition; phosphorylation assays The Journal of cell biology High 20876280
2010 SF2/ASF promotes pri-miR-7 maturation by directly interacting with the primary transcript to facilitate Drosha cleavage, independently of SF2/ASF's splicing function; mature miR-7 in turn targets SF2/ASF 3'UTR to repress its translation, forming a negative feedback loop. miRNA deep sequencing; RNA immunoprecipitation; Drosha cleavage assay; reporter assays for miR-7 targeting; splicing-deficient SF2/ASF mutant analysis Molecular cell High 20385090
2010 Arginine methylation at R93, R97, and R109 of SF2/ASF (by PRMT) controls its subcellular localization; mutations blocking methylation and positive charge cause cytoplasmic accumulation, reducing nuclear alternative splicing activity, increasing translation stimulation, and abrogating NMD enhancement. Site-directed mutagenesis of methylation sites; subcellular fractionation; localization imaging; alternative splicing assays; translation and NMD reporter assays Molecular and cellular biology High 20308322
2010 Akt phosphorylates SRSF1 (SRp30a) at serines 199, 201, 227, and 234 to mediate exclusion of Caspase-9 exon 3,4,5,6 cassette; PI3K/Akt pathway inhibition dramatically increases the Casp9a/9b ratio in NSCLC cells. Site-directed mutagenesis of SRSF1 phosphorylation sites; PI3K/Akt inhibitor treatment; minigene splicing assays Cancer research High 21045158
2011 IL-17 signaling stabilizes CXCL1 mRNA through a pathway involving Act1-TRAF2/TRAF5-SF2/ASF complex formation; SF2/ASF normally binds chemokine mRNA and shortens its half-life, but IL-17 stimulation dissociates SF2/ASF from mRNA, prolonging stability. mRNA stability assays; co-immunoprecipitation of TRAF5-TRAF2-Act1-SF2/ASF complex; SF2/ASF overexpression and siRNA depletion; RNA-IP Nature immunology High 21822258
2011 SRSF1 phosphorylation is modulated by replicational stress via ATM kinase; hyperphosphorylation of SRSF1 in DNA ligase I-deficient cells (which have chronic DNA damage) alters its subnuclear distribution and shifts alternative splicing patterns of target genes. 2D phosphoproteomic analysis; ATM inhibition with caffeine; immunofluorescence localization; RT-PCR of alternative splicing targets Nucleic acids research Medium 21984412
2012 SRSF1 overexpression transforms mammary epithelial cells requiring the first RRM and nuclear functions; SRSF1 promotes alternative splicing of BIM and BIN1 to produce isoforms lacking pro-apoptotic functions; SRSF1 cooperates with MYC to transform cells partly by potentiating eIF4E activation. 3D mammary acinar culture; orthotopic transplantation; alternative splicing analysis; eIF4E/MYC cooperation assays; RRM domain mutant analysis Nature structural & molecular biology High 22245967
2012 SRSF1 is a direct transcriptional target of MYC; MYC activates SRSF1 transcription through two non-canonical E-boxes in the SRSF1 promoter; increased SRSF1 protein mediates alternative splicing of MKNK2 and TEAD1; SRSF1 knockdown reduces MYC's oncogenic activity. ChIP assay showing MYC binding to SRSF1 promoter; promoter reporter assays; MYC knockdown with SRSF1 expression measurement; alternative splicing analysis Cell reports High 22545246
2013 SRSF1 and SRSF9 promote β-catenin accumulation by recruiting β-catenin mRNA and enhancing its translation in an mTOR-dependent manner, contributing to Wnt signaling-mediated tumorigenesis. mRNA-protein co-immunoprecipitation; translation assays; mTOR inhibition; overexpression studies EMBO molecular medicine Medium 23592547
2013 SF2/ASF is recruited to the IL-2 promoter independently of TCR/CD3 signaling, increases IL-2 transcriptional activity, and enhances IL-2 production in SLE T cells; SF2/ASF also limits production of an unstable CD3ζ splice variant to enhance CD3ζ expression. Chromatin immunoprecipitation of SF2/ASF at IL-2 promoter; overexpression with IL-2 promoter reporter; RT-PCR of CD3ζ isoforms Proceedings of the National Academy of Sciences of the United States of America Medium 23319613
2014 SRSF1 binds to a regulatory element in CD6 intron 4 and activates exon 5 splicing; T cell activation causes SRSF1 downregulation, leading to decreased SRSF1 recruitment to CD6 transcript (shown by RNA-IP), combined with increased transcription rate, resulting in exon 5 skipping. RNA immunoprecipitation; chromatin immunoprecipitation; histone deacetylase inhibitor treatment; minigene and overexpression experiments Journal of immunology High 24890719
2014 SRSF1 inhibits HIV-1 Tat transactivation by competing for overlapping binding sequences within TAR RNA; SRSF1 can also increase basal viral transcription in the absence of Tat by recruiting P-TEFb to TAR from the 7SK snRNP. RNA binding competition assays; Tat transactivation reporter assays; P-TEFb co-immunoprecipitation Nucleic acids research Medium 25416801
2017 SRSF1 regulates DBF4B pre-mRNA splicing by binding and promoting inclusion of exon 6; the exon 6-containing isoform (DBF4B-FL) is required for cancer cell proliferation and genomic stability maintenance; DBF4B-FL overexpression rescues DNA damage induced by SRSF1 knockdown. RNA-IP; siRNA knockdown with DNA damage assays; rescue overexpression; tumor growth assays in mice Cell reports High 29262322
2017 SRSF1 promotes VSMC proliferation through a Δ133p53/EGR1/KLF5 pathway: SRSF1 favors induction of the truncated p53 isoform Δ133p53, which transcriptionally activates KLF5 via the Δ133p53-EGR1 complex, accelerating cell-cycle progression. Conditional SMC-specific Srsf1 knockout mice with wire injury model; overexpression in rat arteries; siRNA knockdown in human VSMCs; alternative splicing and pathway analysis Nature communications High 28799539
2018 SRSF1 promotes NMD by increasing UPF1 binding to mRNAs when SRSF1 is positioned downstream of a PTC; this mechanism bypasses UPF2 recruitment, is enhanced by splicing/EJC deposition, and resembles EJC-dependent NMD. UV-crosslinking RNA-IP for UPF1 binding; NMD reporter assays with SRSF1 tethered downstream of PTC; UPF2-independent mechanism demonstration Cell reports High 29768215
2018 PP1 dephosphorylates the SRSF1 RS domain; two electrostatic residues in helix α2 and a hydrophobic residue in helix α1 of RRM1 constitute the PP1 binding surface; disruption of this interaction reduces RS domain phosphorylation and increases SRSF1 diffusion from speckles, shifting alternative splicing patterns. NMR spectroscopy to identify PP1-binding surface; mutagenesis of identified residues; phosphorylation state and localization analysis; alternative splicing assays The Journal of biological chemistry High 30185622
2018 SRSF1 modulates PTPMT1 alternative splicing; reduced SRSF1 upon irradiation favors the short PTPMT1 isoform, which promotes AMPK phosphorylation and DNA double-strand breaks, sensitizing cancer cells to radiation. SRSF1 knockdown with RNA-seq; irradiation assays; AMPK phosphorylation measurement; tumor xenograft radiosensitivity EBioMedicine Medium 30429088
2019 SRSF1 promotes gliomagenesis by switching the alternative splicing of MYO1B to produce a membrane-localized oncogenic isoform (MYO1B-fl) in a position-dependent manner; MYO1B-fl activates PDK1/AKT and PAK/LIMK pathways to increase tumorigenic potential. RNA-Seq; motif analysis; SRSF1 KD with functional rescue using MYO1B isoforms; pathway activation assays; in vivo glioma model The Journal of clinical investigation High 30481162
2019 T cell-restricted Srsf1 knockout mice develop systemic autoimmunity with increased mTORC1 activity and reduced PTEN expression; SRSF1 overexpression rescues PTEN and suppresses mTORC1 activation, establishing a direct SRSF1-PTEN-mTORC1 regulatory axis in T cells. Conditional T cell-specific Srsf1 knockout mice; flow cytometry; mTOR pathway inhibition with rapamycin; SRSF1 overexpression rescue in human T cells The Journal of clinical investigation High 31487268
2021 The RRM1 of SRSF1 binds preferentially to a CN motif (N = any nucleotide); a single E87N mutation in RRM1 enables binding to uridines and activates SMN exon 7 inclusion; the flexible inter-RRM linker allows RRM1 to bind RNA on both sides of the RRM2 binding site, revealing a bimodal mode of interaction. NMR solution structure of RRM1-RNA complex; site-directed mutagenesis (E87N); in vitro and cell-based SMN exon 7 splicing assays; RNA binding measurements Nature communications High 33462199
2021 SRSF1 inhibits autophagy by (1) promoting splicing of Bcl-xL long isoform that interacts with Beclin1 to disrupt the Beclin1-PIK3C3 complex, and (2) directly interacting with PIK3C3 to prevent Beclin1-PIK3C3 interaction; SRSF1 itself is degraded by autophagy via interaction with LC3-II, creating a positive feedback loop. Autophagosome/LC3-II quantification; co-immunoprecipitation of Beclin1-PIK3C3; alternative splicing analysis of Bcl-x; SRSF1-LC3 interaction by co-IP Signal transduction and targeted therapy High 33664238
2021 SRSF1 nucleo-cytoplasmic shuttling is required for efficient translation of a subset of mRNAs involved in multiciliogenesis; a knock-in mouse model with nuclear-retained SRSF1 (NRS insertion) shows small body size, hydrocephalus, and immotile sperm due to ciliary defects. Genome-editing knock-in of nuclear retention signal (NRS) in Srsf1; polyribosome profiling; proteomics; ciliary structure and motility assays eLife High 34338635
2021 SRSF1 is recruited by U1 snRNP via direct contact with U1 snRNA stem-loop 3, independently of exon sequences and separately from ESE binding; this exon-independent recruitment is required for splicing and underlies exon definition. Single-molecule co-localization assays; structural NMR and cross-linking analysis; functional splicing assays with U1 snRNA stem-loop 3 mutations The EMBO journal High 34779515
2021 SRSF1 is essential for hepatocyte function through maintenance of genome stability; SRSF1 depletion in mouse liver causes excessive R-loop formation, DNA damage, global inhibition of mRNA transcription and protein synthesis, lipid accumulation (via impaired lipid metabolism/trafficking), and NASH-like pathology with necroptotic cell death. Liver-specific Srsf1 knockout mice; hepatocyte transcriptome, proteome, RNA-binding analysis (eCLIP); R-loop detection assays; lipid staining; histopathology Nature communications High 36759613
2021 SRSF1-dependent nuclear export of pathological C9ORF72-repeat transcripts is a key mechanism in ALS; partial SRSF1 depletion inhibits repeat RNA nuclear export and confers neuroprotection in C9ORF72-ALS patient-derived neurons and Drosophila models. Gene therapy-based SRSF1 depletion; genome-wide RNA sequencing in patient-derived neurons and Drosophila; functional motor neuron assays Molecular neurodegeneration Medium 34376242
2021 SRSF1 and SRSF7 bind to a clustered motif region (CAR-N) in the intronless lncRNA NKILA to recruit the TREX complex (via UAP56 and ALYREF) for nuclear export via the TAP pathway; removal of CAR-N causes nuclear retention and abolishes NKILA's function in inhibiting breast cancer cell migration. In vitro RNP purification with mass spectrometry; siRNA screening; RNA-IP; protein-IP; knock-in models with CAR-N deletion Nucleic acids research High 34096602
2021 SRSF1 is required for satellite cell proliferation and functional neuromuscular junction (NMJ) formation in postnatal mice; conditional deletion in myogenic progenitors prevents NMJ maturation, causing muscle weakness and premature death, associated with disrupted tissue-specific alternative splicing. Myogenic progenitor-specific Srsf1 conditional knockout mice; RNA-seq of alternative splicing; NMJ morphology and electrophysiology; satellite cell proliferation assays Stem cell reports High 32888503
2023 SRSF1 activates MAPK signaling by upregulating IL1R1 through alternative-splicing-regulated mRNA stability; SRSF1 is destabilized via negative feedback in normal epithelial cells expressing KRASG12D; hyperactive MYC overrides this negative feedback to facilitate PDAC tumorigenesis. Pancreas-specific SRSF1 transgenic and conditional KO mice; KRASG12D mouse model; pancreas organoids; RNA-seq; MAPK pathway assays; MYC-SRSF1 interaction analysis Cancer discovery High 37098965
2023 PRMT1 methylates SRSF1, which is critical for SRSF1 phosphorylation and RNA binding, and promotes exon inclusion in breast cancer; a selective PRMT1 inhibitor (iPRMT1) reduces SRSF1 arginine methylation, exon inclusion, and cancer cell growth. PRMT1 methylome profiling; in vitro methylation assays; phosphorylation assays after PRMT1 inhibition; RNA binding assays; tumor growth assays Cell reports High 37938975
2024 SRSF1 interacts physically with FANCD2 and stimulates FANCD2 monoubiquitination in an RNA-dependent manner; monoubiquitinated FANCD2 in turn is crucial for assembly of the SRSF1-NXF1 nuclear export complex and mRNA export; SRSF1 cancer-associated mutants fail to interact with FANCD2, leading to R-loop accumulation. Co-immunoprecipitation of SRSF1-FANCD2; monoubiquitination assays; mRNA export measurements; R-loop detection; cancer-associated SRSF1 mutant analysis Cell reports High 38165804

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2010 SF1 and SF2 helicases: family matters. Current opinion in structural biology 758 20456941
2007 The gene encoding the splicing factor SF2/ASF is a proto-oncogene. Nature structural & molecular biology 756 17310252
2005 Inactivation of the SR protein splicing factor ASF/SF2 results in genomic instability. Cell 627 16096057
2012 The splicing factor SRSF1 regulates apoptosis and proliferation to promote mammary epithelial cell transformation. Nature structural & molecular biology 360 22245967
2005 Cell motility is controlled by SF2/ASF through alternative splicing of the Ron protooncogene. Molecular cell 319 16364913
2016 Long Noncoding RNA MALAT1 Promotes Hepatocellular Carcinoma Development by SRSF1 Upregulation and mTOR Activation. Cancer research 266 27993818
1993 Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains. The EMBO journal 233 8223480
2008 The splicing factor SF2/ASF regulates translation initiation by enhancing phosphorylation of 4E-BP1. Molecular cell 226 18439897
1997 The splicing factor SRp20 modifies splicing of its own mRNA and ASF/SF2 antagonizes this regulation. The EMBO journal 226 9305649
2011 Treatment with IL-17 prolongs the half-life of chemokine CXCL1 mRNA via the adaptor TRAF5 and the splicing-regulatory factor SF2 (ASF). Nature immunology 203 21822258
1993 Pathways for selection of 5' splice sites by U1 snRNPs and SF2/ASF. The EMBO journal 183 8253084
1993 Functional domains of the human splicing factor ASF/SF2. The EMBO journal 173 8223481
1995 Overexpression of the SR proteins ASF/SF2 and SC35 influences alternative splicing in vivo in diverse ways. RNA (New York, N.Y.) 171 7489505
2012 Oncogenic splicing factor SRSF1 is a critical transcriptional target of MYC. Cell reports 170 22545246
2016 Genomic Landscape Survey Identifies SRSF1 as a Key Oncodriver in Small Cell Lung Cancer. PLoS genetics 169 27093186
1998 Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2. The EMBO journal 167 9799243
2010 SF2/ASF autoregulation involves multiple layers of post-transcriptional and translational control. Nature structural & molecular biology 163 20139984
1997 Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro. RNA (New York, N.Y.) 163 9404896
2010 A splicing-independent function of SF2/ASF in microRNA processing. Molecular cell 160 20385090
2014 MALAT1 promotes cell proliferation in gastric cancer by recruiting SF2/ASF. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 148 24857172
2010 Sam68 regulates EMT through alternative splicing-activated nonsense-mediated mRNA decay of the SF2/ASF proto-oncogene. The Journal of cell biology 143 20876280
1999 atSRp30, one of two SF2/ASF-like proteins from Arabidopsis thaliana, regulates splicing of specific plant genes. Genes & development 135 10215626
2019 Splicing factor SRSF1 promotes gliomagenesis via oncogenic splice-switching of MYO1B. The Journal of clinical investigation 121 30481162
1999 The subcellular localization of SF2/ASF is regulated by direct interaction with SR protein kinases (SRPKs). The Journal of biological chemistry 117 10196197
2010 Alternative splicing of caspase 9 is modulated by the phosphoinositide 3-kinase/Akt pathway via phosphorylation of SRp30a. Cancer research 111 21045158
1997 The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A. The EMBO journal 111 9130721
2008 The splicing-factor oncoprotein SF2/ASF activates mTORC1. Proceedings of the National Academy of Sciences of the United States of America 106 18832178
1994 The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5' splice sites. Proceedings of the National Academy of Sciences of the United States of America 106 7512732
1995 U1 snRNP-ASF/SF2 interaction and 5' splice site recognition: characterization of required elements. Nucleic acids research 103 7667103
2014 Mutual inhibition between YAP and SRSF1 maintains long non-coding RNA, Malat1-induced tumourigenesis in liver cancer. Cellular signalling 102 24468535
2013 SRSF1 and SRSF9 RNA binding proteins promote Wnt signalling-mediated tumorigenesis by enhancing β-catenin biosynthesis. EMBO molecular medicine 101 23592547
2021 SRSF1 inhibits autophagy through regulating Bcl-x splicing and interacting with PIK3C3 in lung cancer. Signal transduction and targeted therapy 97 33664238
2004 Hypophosphorylated ASF/SF2 binds TAP and is present in messenger ribonucleoproteins. The Journal of biological chemistry 97 15184380
2018 SRSF1 modulates PTPMT1 alternative splicing to regulate lung cancer cell radioresistance. EBioMedicine 93 30429088
2010 Arginine methylation controls the subcellular localization and functions of the oncoprotein splicing factor SF2/ASF. Molecular and cellular biology 87 20308322
2017 SRSF1 promotes vascular smooth muscle cell proliferation through a Δ133p53/EGR1/KLF5 pathway. Nature communications 83 28799539
1995 Identification of a plant serine-arginine-rich protein similar to the mammalian splicing factor SF2/ASF. Proceedings of the National Academy of Sciences of the United States of America 80 7644475
2012 Regulation of Mcl-1 by SRSF1 and SRSF5 in cancer cells. PloS one 78 23284704
2010 The A-repeat links ASF/SF2-dependent Xist RNA processing with random choice during X inactivation. Nature structural & molecular biology 72 20657585
2019 Splicing factor SRSF1 controls T cell hyperactivity and systemic autoimmunity. The Journal of clinical investigation 70 31487268
1976 Isolation and properties of the DNA of African swine fever (ASF) virus. The Journal of general virology 65 823295
2017 SRSF1 Prevents DNA Damage and Promotes Tumorigenesis through Regulation of DBF4B Pre-mRNA Splicing. Cell reports 63 29262322
2004 Relative amounts of antagonistic splicing factors, hnRNP A1 and ASF/SF2, change during neoplastic lung growth: implications for pre-mRNA processing. Molecular carcinogenesis 63 15390079
2003 The CD44 alternative v9 exon contains a splicing enhancer responsive to the SR proteins 9G8, ASF/SF2, and SRp20. The Journal of biological chemistry 63 12826680
2010 Identification of ASF/SF2 as a critical, allele-specific effector of the cyclin D1b oncogene. Cancer research 62 20460515
2018 Mechanism of Nonsense-Mediated mRNA Decay Stimulation by Splicing Factor SRSF1. Cell reports 60 29768215
1999 SF2 and SRp55 regulation of CD45 exon 4 skipping during T cell activation. European journal of immunology 59 10092085
2023 Splicing Factor SRSF1 Promotes Pancreatitis and KRASG12D-Mediated Pancreatic Cancer. Cancer discovery 58 37098965
2022 Circular RNA circPVT1 promotes nasopharyngeal carcinoma metastasis via the β-TrCP/c-Myc/SRSF1 positive feedback loop. Molecular cancer 56 36199071
2004 Tra2 beta, SF2/ASF and SRp30c modulate the function of an exonic splicing enhancer in exon 10 of tau pre-mRNA. Genes to cells : devoted to molecular & cellular mechanisms 56 15009090
1996 Novel exploitation of a nuclear function by influenza virus: the cellular SF2/ASF splicing factor controls the amount of the essential viral M2 ion channel protein in infected cells. The EMBO journal 54 8895585
2022 METTL3-stabilized lncRNA SNHG7 accelerates glycolysis in prostate cancer via SRSF1/c-Myc axis. Experimental cell research 53 35405116
2012 Correlation of SRSF1 and PRMT1 expression with clinical status of pediatric acute lymphoblastic leukemia. Journal of hematology & oncology 53 22839530
1997 HIV Rev-dependent binding of SF2/ASF to the Rev response element: possible role in Rev-mediated inhibition of HIV RNA splicing. Proceedings of the National Academy of Sciences of the United States of America 53 9023367
2001 SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain. Journal of molecular biology 50 11575921
2015 Posttranscriptional Regulation of Splicing Factor SRSF1 and Its Role in Cancer Cell Biology. BioMed research international 47 26273603
2013 Splicing factor SF2/ASF rescues IL-2 production in T cells from systemic lupus erythematosus patients by activating IL-2 transcription. Proceedings of the National Academy of Sciences of the United States of America 47 23319613
2021 Structure of SRSF1 RRM1 bound to RNA reveals an unexpected bimodal mode of interaction and explains its involvement in SMN1 exon7 splicing. Nature communications 46 33462199
2010 Multiple ASF/SF2 sites in the human papillomavirus type 16 (HPV-16) E4-coding region promote splicing to the most commonly used 3'-splice site on the HPV-16 genome. Journal of virology 46 20519389
2008 Human papillomavirus type 16 E2 protein transcriptionally activates the promoter of a key cellular splicing factor, SF2/ASF. Journal of virology 46 18945764
2007 Ordered multi-site phosphorylation of the splicing factor ASF/SF2 by SRPK1. Journal of molecular biology 46 18155240
2008 Adaptable molecular interactions guide phosphorylation of the SR protein ASF/SF2 by SRPK1. Journal of molecular biology 44 18687337
2014 HIV-1 transcription is regulated by splicing factor SRSF1. Nucleic acids research 42 25416801
2021 Long noncoding RNA DGCR5 involves in tumorigenesis of esophageal squamous cell carcinoma via SRSF1-mediated alternative splicing of Mcl-1. Cell death & disease 41 34099633
2019 Long non-coding RNA MIR205HG regulates KRT17 and tumor processes in cervical cancer via interaction with SRSF1. Experimental and molecular pathology 41 31655037
2023 Splicing factor SRSF1 deficiency in the liver triggers NASH-like pathology and cell death. Nature communications 40 36759613
2004 RNA recognition motif 2 directs the recruitment of SF2/ASF to nuclear stress bodies. Nucleic acids research 40 15302913
2002 Human splicing factor ASF/SF2 encodes for a repressor domain required for its inhibitory activity on pre-mRNA splicing. The Journal of biological chemistry 38 11801589
2021 Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia function. eLife 37 34338635
2020 Splicing factor SRSF1 controls T cell homeostasis and its decreased levels are linked to lymphopenia in systemic lupus erythematosus. Rheumatology (Oxford, England) 34 32206811
2000 Overexpression of essential splicing factor ASF/SF2 blocks the temporal shift in adenovirus pre-mRNA splicing and reduces virus progeny formation. Journal of virology 33 10982344
2021 Splicing factor SRSF1 is indispensable for regulatory T cell homeostasis and function. Cell reports 32 34233194
2021 Exon-independent recruitment of SRSF1 is mediated by U1 snRNP stem-loop 3. The EMBO journal 32 34779515
2022 circSLC41A1 Resists Porcine Granulosa Cell Apoptosis and Follicular Atresia by Promoting SRSF1 through miR-9820-5p Sponging. International journal of molecular sciences 31 35163432
2015 Dynamic Distribution and Interaction of the Arabidopsis SRSF1 Subfamily Splicing Factors. Plant physiology 31 26697894
2011 Phosphorylation of SRSF1 is modulated by replicational stress. Nucleic acids research 31 21984412
1998 cdc2 kinase-mediated phosphorylation of splicing factor SF2/ASF. Biochemical and biophysical research communications 31 9731229
2023 Targeting PRMT1-mediated SRSF1 methylation to suppress oncogenic exon inclusion events and breast tumorigenesis. Cell reports 30 37938975
2014 T cell activation regulates CD6 alternative splicing by transcription dynamics and SRSF1. Journal of immunology (Baltimore, Md. : 1950) 30 24890719
2009 Regiospecific phosphorylation control of the SR protein ASF/SF2 by SRPK1. Journal of molecular biology 29 19477182
2015 XPB: An unconventional SF2 DNA helicase. Progress in biophysics and molecular biology 28 25641424
2010 Crystal structure of the phage T4 recombinase UvsX and its functional interaction with the T4 SF2 helicase UvsW. Journal of molecular biology 28 21035462
2008 SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells. Journal of thrombosis and haemostasis : JTH 28 18315555
2004 An erythroid differentiation-specific splicing switch in protein 4.1R mediated by the interaction of SF2/ASF with an exonic splicing enhancer. Blood 28 15522963
2019 SRSF1-dependent alternative splicing attenuates BIN1 expression in non-small cell lung cancer. Journal of cellular biochemistry 27 31478261
2013 Structure and Mechanisms of SF2 DNA Helicases. Advances in experimental medicine and biology 27 23161006
2011 Predicting sequence and structural specificities of RNA binding regions recognized by splicing factor SRSF1. BMC genomics 26 22369183
2024 The FANCI/FANCD2 complex links DNA damage response to R-loop regulation through SRSF1-mediated mRNA export. Cell reports 25 38165804
2021 CCL21 activation of the MALAT1/SRSF1/mTOR axis underpins the development of gastric carcinoma. Journal of translational medicine 25 34001131
2021 SRSF1-dependent inhibition of C9ORF72-repeat RNA nuclear export: genome-wide mechanisms for neuroprotection in amyotrophic lateral sclerosis. Molecular neurodegeneration 25 34376242
2005 New talents for an old acquaintance: the SR protein splicing factor ASF/SF2 functions in the maintenance of genome stability. Cell cycle (Georgetown, Tex.) 25 16294036
2021 Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway. Nucleic acids research 24 34096602
2018 Molecular interactions connecting the function of the serine-arginine-rich protein SRSF1 to protein phosphatase 1. The Journal of biological chemistry 24 30185622
2021 Splicing factor SRSF1 limits IFN-γ production via RhoH and ameliorates experimental nephritis. Rheumatology (Oxford, England) 22 32810232
2021 lncRNA LINC01296 Promotes Oral Squamous Cell Carcinoma Development by Binding with SRSF1. BioMed research international 22 34195277
2021 LINC00857 regulated by ZNF460 enhances the expression of CLDN12 by sponging miR-150-5p and recruiting SRSF1 for alternative splicing to promote epithelial-mesenchymal transformation of pancreatic adenocarcinoma cells. RNA biology 22 35442145
2007 Deletion of the N-terminus of SF2/ASF permits RS-domain-independent pre-mRNA splicing. PloS one 22 17786225
2021 Propofol suppresses colorectal cancer development by the circ-PABPN1/miR-638/SRSF1 axis. Analytical biochemistry 21 34453920
2020 Splicing Factor SRSF1 Is Essential for Satellite Cell Proliferation and Postnatal Maturation of Neuromuscular Junctions in Mice. Stem cell reports 21 32888503
2017 miR‑30c suppresses prostate cancer survival by targeting the ASF/SF2 splicing factor oncoprotein. Molecular medicine reports 21 28677791