Affinage

PPP1CA

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit · UniProt P62136

Round 2 corrected
Length
330 aa
Mass
37.5 kDa
Annotated
2026-04-28
101 papers in source corpus 46 papers cited in narrative 46 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PPP1CA encodes the catalytic subunit of protein phosphatase 1-alpha (PP1α), a major serine/threonine phosphatase that dephosphorylates a broad spectrum of substrates—including pRB, Cdk9/P-TEFb, YAP/TAZ, AKT, eIF2α, caspase-9, FOXP3, NCC, TFEB, Keap1, histone H4S47, AMPK, and tau—to regulate cell cycle progression, transcription elongation, Hippo signaling, stress responses, apoptosis, autophagy, ion transport, and memory consolidation (PMID:8384581, PMID:18483222, PMID:21909427, PMID:37605006, PMID:37676724). Substrate specificity and subcellular targeting are conferred by a large family of regulatory/targeting subunits that engage a conserved RVxF-binding groove on the PP1α surface, while 14-3-3ζ binding and PML nuclear body sequestration control its cytoplasmic–nuclear distribution (PMID:11839776, PMID:24956593, PMID:29335436). PP1α catalytic activity is temporally modulated by inhibitory Thr-320 phosphorylation (Cdk2), PKC-mediated Ser-325 phosphorylation, CARM1-dependent Arg-23 methylation, SUV39H2-dependent Lys-141 methylation, and reversible GSH-mediated disulfide formation that protects against irreversible oxidation (PMID:10506210, PMID:9399575, PMID:37649137, PMID:37605006, PMID:30531830). PP1α is also co-opted by viral proteins—HSV-1 γ₁34.5 and Zika NS2B redirect it to dephosphorylate eIF2α, overcoming host translational arrest and stress granule formation to promote viral replication (PMID:26621722, PMID:38935808).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1984 High

    Establishing PP1 as a signal-regulated phosphatase: discovery that phospho-DARPP-32 potently inhibits PP1 at nanomolar concentrations placed PP1 downstream of dopamine/cAMP signaling and demonstrated that endogenous inhibitor proteins gate PP1 activity.

    Evidence Biochemical inhibition assay with purified PP1 and phospho-DARPP-32

    PMID:6087160

    Open questions at the time
    • Isoform specificity of DARPP-32 inhibition (PP1α vs. PP1β/γ) not resolved
    • In vivo stoichiometry and tissue-specific relevance not addressed
  2. 1993 High

    Linking PP1α to cell cycle control: identification of PP1α as a direct pRB-binding partner that preferentially associates with hypophosphorylated pRB from mitosis to early G1 established PP1α as a candidate pRB-activating phosphatase.

    Evidence Yeast two-hybrid screen, in vitro binding, and cell cycle synchronization in mammalian cells

    PMID:8384581

    Open questions at the time
    • Direct dephosphorylation of pRB by PP1α not demonstrated in this study
    • Regulatory subunit mediating the pRB–PP1α interaction unidentified
  3. 1999 High

    Resolving how PP1α is temporally restrained during S-phase entry: Cdk2-mediated Thr-320 phosphorylation inhibits PP1α at G1/S, preventing premature pRB dephosphorylation and establishing a feedback loop between the cell cycle kinase and phosphatase arms.

    Evidence Phospho-specific antibodies, in vitro Cdk2 kinase assay, co-IP with pRB, cell cycle synchronization

    PMID:10506210

    Open questions at the time
    • Phosphatase that reverses Thr-320 not identified
    • Whether Thr-320 phosphorylation affects PP1α activity toward non-pRB substrates unclear
  4. 2000 High

    Extending PP1α to cardiac physiology: demonstration that PP1 resides in the RyR2 macromolecular complex on the sarcoplasmic reticulum linked PP1 to excitation–contraction coupling and heart failure pathophysiology.

    Evidence Co-sedimentation, co-IP, and single-channel recordings in human heart

    PMID:10830164

    Open questions at the time
    • Relative contributions of PP1α vs. other PP1 isoforms at RyR2 not resolved
    • Direct dephosphorylation sites on RyR2 not mapped in this study
  5. 2002 High

    Defining the modular targeting logic of PP1: comprehensive analysis established that >50 regulatory subunits engage the conserved RVxF-binding groove on PP1c to direct it to distinct substrates and subcellular compartments, providing the central paradigm for PP1 specificity.

    Evidence Structural analysis, biochemical interaction mapping, and mutational studies across multiple labs (review synthesis)

    PMID:11839776

    Open questions at the time
    • Many targeting subunits lack identified substrates
    • How combinatorial regulation by multiple regulators is integrated remains unclear
  6. 2006 High

    Connecting PP1α to apoptotic signaling: PP1α dephosphorylates caspase-9 at Thr125 upon IL-2 withdrawal, activating the intrinsic apoptosis cascade in T cells and revealing two novel PP1α-binding sites on caspase-9.

    Evidence In vitro and in vivo co-IP, cell-free caspase activation assay, siRNA/inhibitor experiments

    PMID:16888006

    Open questions at the time
    • Identity of the regulatory subunit targeting PP1α to caspase-9 unknown
    • Generalizability beyond IL-2-dependent T cells not tested
  7. 2008 High

    Revealing PP1α as a transcription elongation regulator: PP1α dephosphorylates Cdk9 T-loop (Thr186) cooperatively with PP2B-mediated conformational priming of 7SK snRNP, releasing P-TEFb to activate RNA Pol II elongation.

    Evidence Co-IP, in vitro phosphatase assay, siRNA knockdown in UV/HMBA-stimulated cells

    PMID:18483222

    Open questions at the time
    • Whether PP1α acts constitutively or only under stress/signal-induced conditions unclear
    • Regulatory subunit mediating PP1α recruitment to 7SK snRNP not identified
  8. 2011 High

    Placing PP1α in Hippo pathway regulation: PP1α directly dephosphorylates YAP at Ser127, promoting its nuclear accumulation and transcriptional activation—a finding subsequently confirmed for TAZ in Wnt signaling and extended to hepatocellular carcinoma and melanoma contexts.

    Evidence Co-IP, in vitro dephosphorylation, okadaic acid, subcellular fractionation, reporter assays; replicated for TAZ (2014) and in HCC (2025)

    PMID:21909427 PMID:24510127 PMID:40513845

    Open questions at the time
    • Whether a specific regulatory subunit directs PP1α to YAP/TAZ or whether LACTB competition is the primary control is unresolved
    • LATS-independent vs. LATS-dependent contributions not fully dissected
  9. 2013 High

    Identifying PP1α as a chromatin regulator: PP1α dephosphorylates histone H4 at Ser47 and interacts with the H4S47 kinase Pak2, controlling histone H3.3 deposition and linking PP1α to epigenetic regulation.

    Evidence Co-IP, in vitro binding, ChIP, siRNA depletion, mass spectrometry

    PMID:23828041

    Open questions at the time
    • Genomic loci most affected by PP1α-dependent H3.3 deposition not mapped
    • Functional consequences for gene expression programs not characterized
  10. 2014 Medium

    Defining cytoplasmic retention as a major mode of PP1α regulation: 14-3-3ζ binds PP1α (residues 159–279) independently of phosphorylation and retains it in the cytosol; disruption causes nuclear PP1α accumulation, establishing subcellular partitioning as a regulatory axis.

    Evidence Co-IP, GST pulldown, deletion mapping, dominant-negative 14-3-3ζ, subcellular fractionation

    PMID:24956593

    Open questions at the time
    • Signals that release PP1α from 14-3-3ζ under physiological conditions not defined
    • Whether 14-3-3ζ binding is isoform-specific for PP1α over PP1β/γ untested
  11. 2015 High

    Demonstrating viral hijacking of PP1α for pathogenesis: HSV-1 γ₁34.5 redirects PP1α to dephosphorylate eIF2α, overcoming translational arrest; this is essential for disseminated neonatal disease, defining PP1α as a host factor exploited by viruses.

    Evidence PP1α-binding mutant virus, IFN-KO mice, pharmacological eIF2α dephosphorylation inhibition, brain slice culture

    PMID:26621722

    Open questions at the time
    • Whether host regulatory subunits compete with γ₁34.5 for PP1α binding in vivo not assessed
    • Contribution of PP1α vs. PP1γ to viral eIF2α targeting unclear
  12. 2017 High

    Integrating PP1α into MAPK signaling and tumor suppression: PP1α activates B-Raf via dephosphorylation in a S6K-dependent pathway, and PML sequesters PP1α in nuclear bodies to repress this activity; PPP1CA amplification in metastatic prostate cancer established its oncogenic potential.

    Evidence Co-IP, phosphorylation assays, subcellular fractionation, MAPK assays, human cancer genomics, xenograft

    PMID:29335436

    Open questions at the time
    • Direct B-Raf dephosphorylation sites by PP1α not mapped
    • Frequency and clinical impact of PPP1CA amplification across cancer types not comprehensively analyzed
  13. 2018 Medium

    Uncovering redox-mediated self-regulation: PP1α undergoes transient GSH-mediated disulfide formation (Cys39–Cys127) that reversibly inactivates it under oxidative stress, protecting against irreversible over-oxidation.

    Evidence Cross-over-read mass spectrometry for disulfide identification, activity assays under defined redox conditions

    PMID:30531830

    Open questions at the time
    • In vivo relevance of this disulfide mechanism not demonstrated
    • Whether other PP1 isoforms share this redox sensitivity unknown
  14. 2019 High

    Revealing post-translational control of PP1α protein levels: USP11 deubiquitinates PP1α to prevent proteasomal degradation, stabilizing PP1α and promoting ERK/MAPK signaling in colorectal cancer.

    Evidence LC-MS/MS, co-IP, ubiquitination assays, in vitro and in vivo tumor models

    PMID:31521612

    Open questions at the time
    • E3 ubiquitin ligase responsible for PP1α ubiquitination not identified
    • Whether USP11-mediated stabilization is tissue-specific unclear
  15. 2023 High

    Discovering lysine methylation as a substrate-selective switch: SUV39H2 mono-methylates PP1α at K141, disrupting its interaction with TFEB via regulatory subunit PPP1R9B, blocking TFEB nuclear translocation and autophagy—establishing post-translational methylation as a mechanism that rewires PP1α substrate specificity.

    Evidence Proteomic analysis, co-IP, in vitro dephosphorylation, K141 methylation identification, autophagy flux assays, in vivo disc degeneration model

    PMID:37605006

    Open questions at the time
    • Whether K141 methylation affects other PP1α substrates besides TFEB not tested
    • Demethylase that reverses K141 methylation unidentified
  16. 2023 High

    Expanding PP1α to renal ion homeostasis and blood pressure control: dietary potassium upregulates PP1α expression and suppresses its inhibitor I-1 (PPP1R1A) in the kidney, enabling direct PP1α-mediated dephosphorylation of the NaCl cotransporter (NCC) independently of WNK/SPAK.

    Evidence Transcriptomic screen, constitutively active SPAK knockin mice, direct PP1α–NCC co-IP, potassium manipulation, blood pressure measurement

    PMID:37676724

    Open questions at the time
    • Regulatory subunit targeting PP1α to NCC not identified
    • Whether this pathway operates in humans not confirmed
  17. 2024 High

    Confirming viral exploitation as a general strategy: Zika NS2B scaffolds PP1α to eIF2α, promoting dephosphorylation that inhibits stress granule formation and enhances viral replication—paralleling the HSV-1 γ₁34.5 mechanism and generalizing PP1α as a convergent target for viral immune evasion.

    Evidence Co-IP, NS2B mutagenesis, stress granule imaging, brain organoid model, viral replication assays

    PMID:38935808

    Open questions at the time
    • Whether other flaviviruses use a similar NS2B–PP1α mechanism not tested
    • Structural basis of NS2B–PP1α interaction unresolved
  18. 2025 Medium

    NY-ESO-1 recruits OTUB1 deubiquitinase to PP1α, forming a ternary complex that stabilizes PP1α and activates ERK to promote anoikis resistance and metastasis—extending the theme of PP1α protein level regulation by deubiquitinases.

    Evidence Co-IP, ubiquitination assays, OTUB1 catalytic mutants, PP1α knockdown, in vivo metastasis model

    PMID:41053024

    Open questions at the time
    • Whether OTUB1 and USP11 act redundantly or on different PP1α pools is unknown
    • Specific ubiquitin chain types removed by OTUB1 from PP1α not characterized

Open questions

Synthesis pass · forward-looking unresolved questions
  • Despite extensive characterization of individual PP1α–substrate interactions, how combinatorial post-translational modifications (Thr-320 phosphorylation, R23 methylation, K141 methylation, Cys39–Cys127 disulfide) are integrated to produce context-dependent substrate selectivity remains undefined, and a comprehensive structural model of a holo-PP1α complex with simultaneous regulatory subunit and substrate engagement is lacking.
  • No structure of a ternary PP1α–regulatory subunit–substrate complex
  • How multiple post-translational modifications crosstalk on PP1α to tune activity is untested
  • Systematic identification of the E3 ligase(s) responsible for PP1α turnover is incomplete

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 17
Localization
GO:0005634 nucleus 5 GO:0005829 cytosol 4 GO:0005886 plasma membrane 2 GO:0005739 mitochondrion 1 GO:0005815 microtubule organizing center 1
Pathway
R-HSA-162582 Signal Transduction 14 R-HSA-1640170 Cell Cycle 3 R-HSA-1643685 Disease 2 R-HSA-168256 Immune System 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-74160 Gene expression (Transcription) 2 R-HSA-4839726 Chromatin organization 1 R-HSA-9612973 Autophagy 1
Partners
GADD34LIMS1MYO18APPP1R1BRB1USP11YAP1YWHAZ
Complex memberships
7SK snRNP/P-TEFbGADD34/PP1α/eIF2αRyR2 macromolecular complexSmad4-MYO18A-PP1A

Evidence

Reading pass · 46 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 PPP1CA (PP1α) was identified as a binding partner of the retinoblastoma protein (pRB) using yeast two-hybrid screening and in vitro binding assays. PP1α isoforms preferentially bind the hypophosphorylated form of pRB, and this association occurs from mitosis to early G1, suggesting PP1α regulates pRB function through the cell cycle. Yeast two-hybrid, in vitro binding assay, cell cycle synchrony experiments Genes & development High 8384581
1984 DARPP-32 (a dopamine-regulated neuronal phosphoprotein) is a potent inhibitor of PP1 (protein phosphatase-1, including PP1α) at nanomolar concentrations, and this inhibition is active only in the phosphorylated form of DARPP-32, establishing PP1 as a key effector downstream of dopamine signaling. Biochemical inhibition assay with purified proteins Nature High 6087160
1999 PPP1CA (PP1α) is phosphorylated at Thr-320 during M-phase and again in late G1 through early S-phase by Cdk2 (but not Cdk4) in a pRB-dependent manner. This inhibitory phosphorylation is required to prevent untimely pRB reactivation during the G1/S transition; PP1α phosphorylated at Thr-320 co-precipitates with pRB during G1/S. In vivo phosphorylation with isoform-specific antibodies, in vitro Cdk2 kinase assay, co-immunoprecipitation, cell cycle synchronization The Journal of biological chemistry High 10506210
2000 PP1 (including PPP1CA) is a component of the macromolecular RyR2 signaling complex on the sarcoplasmic reticulum, demonstrated by co-sedimentation and co-immunoprecipitation. PKA phosphorylation of RyR2 regulates channel open probability, and in failing human hearts RyR2 is PKA hyperphosphorylated with defective channel function. Co-sedimentation, co-immunoprecipitation, single-channel recordings Cell High 10830164
2002 PP1 (PPP1CA) is required for β-adrenergic regulation of the KCNQ1-KCNE1 (IKS) potassium channel. PP1 is targeted to hKCNQ1 through the anchoring protein yotiao via a leucine zipper motif. Disruption of this interaction by an LQTS mutation (hKCNQ1-G589D) impairs channel regulation. Co-immunoprecipitation, mutagenesis, electrophysiology Science High 11799244
2002 PP1 (PPP1CA catalytic subunit) is recruited to substrates through over fifty regulatory/targeting subunits that interact with a conserved RVxF motif-binding hydrophobic groove on the PP1c surface. This targeting mechanism directs PP1c to specific subcellular locations and modulates its activity/specificity. Biochemical interaction mapping, structural analysis of PP1c complexes, mutational analysis Journal of cell science High 11839776
2003 PP1 (including PPP1CA) resides in a mitochondrial macromolecular complex with BAD, PKA, WAVE-1, and glucokinase in liver. BAD is required to assemble this complex; its phosphorylation status regulates glucokinase activity and integrates glycolysis with apoptosis. Proteomic analysis, co-immunoprecipitation, genetics (Bad-deficient hepatocytes), functional metabolic assays Nature High 12931191
2005 PP1 (including PPP1CA) dephosphorylates tau at multiple sites (Ser199, Ser202, Thr205, Thr212, Ser214, Ser235, Ser262, Ser396, Ser404, Ser409) in vitro, contributing approximately 11% of total tau phosphatase activity in human brain, with Km values of 8-12 μM. In vitro phosphatase assay with purified proteins and phosphorylation site-specific antibodies, Km determination The European journal of neuroscience High 16262633
2008 PPP1CA (PP1α) acts cooperatively with PP2B downstream of Ca2+ signaling to release P-TEFb from the 7SK snRNP complex. PP1α dephosphorylates phospho-Thr186 in the Cdk9 T-loop, releasing P-TEFb to activate RNA Pol II transcription elongation. PP2B acts first to induce a conformational change in 7SK snRNP that facilitates PP1α access. Co-immunoprecipitation, in vitro phosphatase assay, siRNA knockdown, analysis of UV/HMBA-induced P-TEFb release Genes & development High 18483222
2008 PPP1CA contributes to oncogenic Ras-induced senescence by promoting pRb dephosphorylation downstream of ceramide accumulation. Loss of PPP1CA function (via antisense RNA or shRNA) bypasses ras/p53-induced growth arrest; PPP1CA shRNA impairs p53-dependent p21 induction after DNA damage and blocks pRb dephosphorylation. Retroviral genetic screen, shRNA knockdown, cell cycle analysis, ceramide treatment Carcinogenesis Medium 18204081
2008 Drosophila Uri (a conserved molecular chaperone) binds PP1α with much higher affinity than PP1β, and this PP1α-selective binding is conserved in humans. Uri is the first PP1α-specific binding protein identified in Drosophila; uri mutants show transcriptional defects, reduced cell viability in the germline, and accumulation of DNA damage. Binding affinity assays, loss-of-function genetics in Drosophila, immunostaining BMC molecular biology Medium 18412953
2008 Hippocampal knockdown of PP1 (PPP1CA) by siRNA injection in mice was sufficient to enhance contextual and temporal long-term memory formation, demonstrating that PP1 acts as a negative regulator of memory consolidation in vivo. In vivo siRNA injection into hippocampus, contextual fear conditioning behavioral assay Genes, brain, and behavior Medium 19191880
2010 PINCH1 directly binds PP1α (PPP1CA) and inhibits its phosphatase activity, resulting in increased Akt1 phosphorylation and enhanced radioresistance. PINCH1-mediated inhibition of PP1α perpetuates Akt1 activity in tumor cells adhered to ECM. Co-immunoprecipitation, in vitro phosphatase activity assay, siRNA knockdown, in vitro and in vivo irradiation experiments The Journal of clinical investigation High 20530873
2011 PP1A (PPP1CA) directly interacts with and dephosphorylates YAP2 at Ser127 in vitro and in vivo, promoting YAP2 nuclear accumulation and transcriptional activation. Inhibition of PP1 by okadaic acid increases YAP2 phosphorylation and cytoplasmic retention. PP1A-mediated YAP2 dephosphorylation enhances cell survival in ovarian cancer cells. Co-immunoprecipitation, in vitro dephosphorylation assay, okadaic acid inhibition, subcellular fractionation, transcriptional reporter assay PloS one High 21909427
2011 Taperin (C9orf75), encoded by a nonsyndromic deafness gene, preferentially docks PP1α (over PP1β/γ) through a classic RVxF motif and suppresses PP1α general phosphatase activity. Taperin-PP1α complexes are found in both nucleus and cytoplasm. SILAC mass spectrometry revealed taperin associates with DNA damage response proteins Ku70, Ku80, PARP, and topoisomerases, and taperin is actively recruited to sites of DNA damage. Biochemical purification, co-immunoprecipitation, SILAC mass spectrometry, phosphatase activity assay, mutagenesis of RVxF motif, live-cell imaging of DNA damage recruitment Biology open High 23213405
2013 PP1α (PPP1CA), PP1β, and Wip1 phosphatases regulate histone H4 Ser47 phosphorylation (H4S47ph) levels and thereby control deposition of histone variant H3.3. PP1α and PP1β bind H3-H4 in vitro and in vivo and also interact with the H4S47 kinase Pak2, suggesting they regulate both Pak2 activity and direct H4S47 dephosphorylation. Depletion of PP1α increases H3.3 occupancy and deposition. Co-immunoprecipitation, in vitro binding assay, ChIP, siRNA depletion, mass spectrometry Nucleic acids research High 23828041
2013 In the ERK/PP1α/PLB/SERCA2a pathway, luteolin activates ERK1/2 which upregulates PP1α expression, leading to phospholamban (PLB) upregulation and relief of SERCA2a inhibition, improving cardiomyocyte contractile function after ischemia/reperfusion injury. Isolated heart and cardiomyocyte functional assay, western blotting for phosphorylation status, pharmacological inhibitors (PD98059, SP600125), rat I/R model PloS one Medium 24386130
2014 PP1A (PPP1CA) mediates canonical Wnt3a-induced TAZ dephosphorylation during osteogenic differentiation. Wnt3a signaling (through GSK3β inhibition) facilitates TAZ dephosphorylation by PP1A, which stabilizes TAZ, prevents 14-3-3 binding, and induces nuclear localization. Depletion of PP1A blocks Wnt3a-induced TAZ stabilization and osteoblast differentiation. siRNA depletion, western blotting for phospho-TAZ, subcellular fractionation, co-immunoprecipitation, differentiation assays Cell death and differentiation High 24510127
2014 14-3-3ζ binds PPP1CA (PP1α) within residues 159-279 (independent of PP1α phosphorylation status) and retains PP1α in the cytosol, thereby regulating its nuclear trafficking. Expression of dominant-negative 14-3-3ζ (K49E) causes PP1α nuclear accumulation. 14-3-3ζ binding does not affect PP1α catalytic activity. Co-immunoprecipitation, GST pulldown, deletion mutant mapping, subcellular fractionation, dominant-negative expression Archives of biochemistry and biophysics Medium 24956593
2005 PP1 inhibitor-3 (Inh3) co-localizes with PPP1CA (PP1α) specifically at centrosomes (not nucleoli) in interphase HEK293 cells, and co-immunoprecipitates with PP1α but not PP1β in vitro. Nucleolar localization of Inh3 is with PP1γ1. This demonstrates isoform-specific subcellular targeting of Inh3 to different PP1 complexes. Immunofluorescence co-localization, co-immunoprecipitation, site-directed mutagenesis of localization signals, laser scanning cytometry Archives of biochemistry and biophysics Medium 16256067
2006 PP1α (PPP1CA) is identified as a regulator of caspase-9 activation. In IL-2-proliferating T cells, phosphorylated PP1α associates with phosphorylated caspase-9 (at Thr125). IL-2 deprivation induces PP1α dephosphorylation and activation, which then dephosphorylates caspase-9 Thr125 causing caspase-9 (and caspase-3) activation. PP1α associates with caspase-9 in vitro and in vivo; two novel PP1α-binding sites on caspase-9 were identified. Co-immunoprecipitation in vivo and in vitro, cell-free caspase activation assay, siRNA/phosphatase inhibitor experiments, identification of novel binding motifs Journal of immunology High 16888006
2013 FOXP3 transcriptional activity in regulatory T cells is regulated by dephosphorylation at Ser418 by protein phosphatase 1 (PP1, including PPP1CA). TNF-α in rheumatoid arthritis synovium induces PP1 expression and activity, leading to FOXP3 Ser418 dephosphorylation and impaired Treg cell function. Western blotting with phospho-specific antibodies, PP1 activity assay, siRNA knockdown, functional Treg suppression assay, patient samples Nature medicine High 23396208
2015 HSV-1 γ34.5 protein targets host PP1α (PPP1CA) to reverse translational arrest; this PP1α-targeting is required for HSV-1 to cause disseminated disease in neonatal mice. The PP1α-binding mutant of HSV-1 replicated in visceral organs early but failed to cause full disease. PP1α targeting by γ34.5 mediates eIF2α dephosphorylation to overcome the integrated stress response. Murine neonatal infection model, PP1α-binding mutant virus, type I IFN-deficient mice, pharmacological inhibition of eIF2α dephosphorylation in brain slice culture PNAS High 26621722
2016 PP1α (PPP1CA) was identified as a negative regulator of TRPV1 channel activity through an HSV-based cDNA library screen. Vectors expressing PP1α reduced thermal sensitivity in rat footpads following virus injection, demonstrating that PP1α-mediated negative regulation of TRPV1 is functional in vivo with specificity for thermal pain. HSV-based library screen for TRPV1 inhibitors, in vitro capsaicin-based selection, in vivo thermal pain behavioral assay Molecular therapy. Methods & clinical development Medium 27382601
2017 PP1α (PPP1CA) acts as a B-Raf activating phosphatase in a S6K/PP1α/B-Raf signaling pathway leading to MAPK activation. PML tumor suppressor sequesters PP1α into PML nuclear bodies, repressing S6K-dependent PP1α phosphorylation, 14-3-3 binding, and cytoplasmic accumulation. PPP1CA gene amplification is enriched in metastatic prostate cancer, and loss of PML derepresses this pathway. Co-immunoprecipitation, phosphorylation assays, subcellular fractionation, MAPK activity assays, genomic analysis of human cancer, mouse xenograft models Nature communications High 29335436
2017 Oxidative stress (arsenite) promotes SIRT1 recruitment to the GADD34/PP1α complex to form a cytoplasmic GADD34/PP1α/eIF2α/SIRT1 complex. The GADD34/PP1α complex dephosphorylates both eIF2α (pSer51) and SIRT1 (pSer47); SIRT1 dephosphorylation activates its deacetylase activity. GADD34 mutants unable to bind PP1α fail to restore eIF2α or SIRT1 dephosphorylation. Mass spectrometry, co-immunoprecipitation, in vitro phosphatase assay, GADD34 knockout MEFs, rescue experiments with PP1α-binding mutant Cell death and differentiation High 28984870
2018 The reduced activity of PP-1α under redox/oxidative stress is caused by transient disulfide bond formation (involving Cys39 and Cys127 in a dimerization event) mediated by glutathione (GSH). This shelters PP-1α against irreversible over-oxidation. The disulfide forms preferentially in the absence of substrate and the electron transport proceeds from the PP-1α catalytic core to the surface. Cross-over-read mass spectrometry for disulfide identification, biochemical activity assays under redox conditions, GSH treatment Scientific reports Medium 30531830
2019 USP11 deubiquitinase stabilizes PPP1CA by deubiquitinating it and protecting it from proteasome-mediated degradation. The USP11/PPP1CA complex promotes colorectal cancer progression by activating the ERK/MAPK signaling pathway. LC-MS/MS protein interaction identification, co-immunoprecipitation, ubiquitination assays, in vitro and in vivo tumor assays EBioMedicine High 31521612
2019 PP1A (PPP1CA) interacts with and dephosphorylates the transcriptional cofactor NACA at Thr-89, Ser-151, and Thr-174. NACA dephosphorylation increases its nuclear localization and, together with PP1A, cooperatively potentiates cJUN transcriptional activity of AP-1 target genes. This is required for osteoblast differentiation and matrix mineralization. Affinity purification mass spectrometry, co-immunoprecipitation with regulatory subunits (PPP1R9B, PPP1R12A, PPP1R18), MS-based phosphorylation mapping, Ala/Asp NACA mutants, transcriptional reporter assays, MC3T3-E1 osteoblast differentiation The Journal of biological chemistry High 30948508
2019 Cavin-3 released from caveolae upon UV stress interacts with and inhibits PP1α (PPP1CA), resulting in increased H2AX phosphorylation and promotion of apoptosis. This defines a pro-apoptotic signaling pathway from plasma membrane caveolae to the nucleus via PP1α. Biotin affinity proteomics, mass spectrometry, cell-free expression system, protein-protein binding assays, co-immunoprecipitation, functional apoptosis assays Nature communications High 31332168
2019 MIIP (migration and invasion inhibitory protein) interacts with PP1α (PPP1CA) via its C-terminal domain and enhances PP1-mediated AKT dephosphorylation, suppressing AKT-mTOR signaling in prostate cancer. Silencing PP1α reverses MIIP's inhibitory effect on AKT phosphorylation and cell growth; MIIPC lacking the PP1α-interaction domain loses its function. Western blot, immunofluorescence co-localization, co-immunoprecipitation, domain deletion mutants, PP1α siRNA, xenograft tumor model Cell communication and signaling Medium 31092266
2021 The Smad4-MYO18A-PP1A complex dephosphorylates PAK1 at Thr423, which prevents PAK1-mediated phosphorylation of β-catenin at Ser675 and its nuclear translocation. MYO18A serves as the PP1-interacting protein for substrate recognition, binding PP1A via an RVFFR motif and Smad4 via its CC domain. LC-MS/MS, co-immunoprecipitation, biochemical dephosphorylation assays, RVFFR motif mutagenesis, in vitro and in vivo functional assays Cell death and differentiation High 34799729
2021 SRPK1/2 and PP1α exert opposing functions on SRSF1 phosphorylation and nuclear translocation, which governs MKNK2 alternative splicing in colon adenocarcinoma. Decreased PP1α activity leads to enhanced SRSF1 phosphorylation and nuclear localization, promoting MKNK2b isoform expression. Western blotting, PCR-based splicing assays, kinase/phosphatase inhibitor treatment, siRNA knockdown, xenograft models Journal of experimental & clinical cancer research Medium 33602301
2021 SNORD12B snoRNA interacts with PP-1α (PPP1CA) and disrupts the interaction between 14-3-3ζ and PP-1α, abolishing 14-3-3ζ-mediated cytoplasmic retention of PP-1α and causing its translocation to the nucleus. Nuclear sequestration of PP-1α reduces AKT dephosphorylation in the cytosol and activates AKT-mTOR-4EBP1 signaling to promote esophageal squamous cell carcinoma progression. Co-immunoprecipitation, subcellular fractionation, RNA immunoprecipitation, western blotting for phospho-AKT, in vitro and in vivo tumor models Oncogene Medium 33941854
2021 LACTB directly binds PP1A (PPP1CA) and attenuates the PP1A-YAP interaction, resulting in decreased YAP dephosphorylation, increased phospho-YAP (Ser127), and prevention of YAP nuclear translocation, suppressing melanoma progression in a LATS1-independent manner. Co-immunoprecipitation, western blotting for phospho-YAP, subcellular fractionation, overexpression/knockdown functional assays, in vivo xenograft Cancer letters Medium 33675985
2023 CARM1 methyltransferase methylates PPP1CA at arginine 23 (R23), which affects PPP1CA-mediated dephosphorylation of AKT at Thr450 and AMPK at Thr172, leading to increased glycolytic flux (via phosphofructokinase-1 and PFKFB3 activation), promoting osteogenic differentiation and inhibiting osteoclastic differentiation. In vitro methylation assay identifying R23 methylation site, CRISPR/Cas9 knockout, lentiviral overexpression, transcriptomics, metabolomics, western blotting, in vivo osteoporosis model Clinical and translational medicine High 37649137
2023 SUV39H2 methyltransferase mono-methylates PPP1CA at K141, disrupting its interaction with TFEB (a newly identified PPP1CA substrate) and blocking TFEB dephosphorylation and nuclear translocation, leading to autophagy deficiency and nucleus pulposus cell senescence in intervertebral disc degeneration. PPP1R9B (neurabin II) serves as a regulatory subunit facilitating PPP1CA-TFEB targeting. Proteomic analysis, co-immunoprecipitation, in vitro dephosphorylation, K141 methylation-site identification, TFEB nuclear translocation assays, autophagy flux assays, in vivo disc degeneration model Cell death and differentiation High 37605006
2023 Dietary potassium upregulates Ppp1Ca (PP1A) expression in the kidney and its negative regulator Ppp1r1a is suppressed. PP1A directly binds to and dephosphorylates the NaCl cotransporter (NCC), and this dephosphorylation is potassium-regulated and occurs in a WNK/SPAK-independent manner. Potassium-dependent suppression of inhibitory subunit 1 (I1) amplifies PP1A activity toward NCC, providing a mechanism for high-K+ diet to lower blood pressure. Transcriptomics screen, constitutively active SPAK knockin mice, direct PP1A-NCC co-immunoprecipitation, potassium manipulation experiments, blood pressure measurements The Journal of clinical investigation High 37676724
2024 A peptide (PDHK1-241aa) encoded by circPDHK1 interacts with PPP1CA and causes its relocalization to the nucleus, thereby inhibiting AKT dephosphorylation in the cytosol and activating the AKT-mTOR signaling pathway to promote clear cell renal cell carcinoma progression. IP-MS, immunofluorescence, western blot, subcellular fractionation, siRNA/lentiviral expression, co-immunoprecipitation, xenograft and metastasis models Molecular cancer Medium 38360682
2024 Zika virus NS2B acts as a scaffold mediating interaction between PP1α (PPP1CA) and eIF2α, promoting PP1α-dependent dephosphorylation of eIF2α to inhibit stress granule formation and boost viral replication. The NS2B-PP1α complex is resistant to ubiquitin-induced degradation. NS2BV35A mutant interacting only with eIF2α fails to inhibit stress granule formation. Co-immunoprecipitation, phosphorylation assays (phospho-eIF2α western blot), NS2B mutagenesis, stress granule imaging, brain organoid model, viral replication assays PLoS pathogens High 38935808
1997 B-cell receptor (BCR) stimulation via surface IgM transiently decreases PP1 activity (predominantly PP1α). PP1α undergoes unique phosphorylation changes after BCR stimulation and PMA treatment; PP1α is a better substrate for PKC than PP1γ isoforms, being phosphorylated by PKC at Ser-325 in its C-terminal region. PP1δ does not contain this phosphorylation site. This suggests PP1α is specifically involved in BCR signal transduction via PKC-mediated phosphorylation. Phosphatase activity assay, 2D electrophoresis/western blot, in vitro PKC phosphorylation assay with C-terminal peptides, subcellular distribution analysis Journal of biochemistry Medium 9399575
2025 NY-ESO-1 acts as a scaffold to recruit deubiquitinase OTUB1 to PP1α (PPP1CA), forming a ternary complex that prevents PP1α polyubiquitination and promotes its stability. OTUB1 deubiquitinase catalytic activity (not its E2-suppressing function) is required to stabilize PP1α. Accumulated PP1α then activates downstream ERK1/2 to promote anoikis resistance and tumor metastasis. Knockdown of PP1α antagonizes NY-ESO-1-mediated anoikis resistance. Co-immunoprecipitation, ubiquitination assays, OTUB1 catalytic mutants, PP1α knockdown, soft agar colony formation, lung metastasis mouse model Cell death & disease Medium 41053024
2025 IIIG9 (PP1R32) forms a protein complex with PP1α (PPP1CA) at adherens junctions and cilia in polarized ependymal and MDCK cells. FRET analysis confirmed high-efficiency interaction between IIIG9 and PP1α in the cytoplasm and near the plasma membrane. The IIIG9-PP1α interaction at adherens junctions is proposed to regulate cell polarity. RT-qPCR with laser microdissection, confocal co-localization, FRET analysis, proximity ligation assay (PLA), co-immunoprecipitation Fluids and barriers of the CNS Medium 41566508
2025 PP1A removes activation-loop phosphorylation from AMPK (α-subunit) while AMPK autophosphorylation sites remain protected, establishing an asymmetric phosphatase control that creates persistent regulatory states in kinase activation. Hybrid precision mass spectrometry (intact mass + bottom-up + top-down MS), in vitro phosphatase competition assay bioRxiv preprintpreprint Medium bio_10.1101_2025.10.10.681638
2025 PPP1CA interacts with YAP1 and dephosphorylates it, inducing YAP1 nuclear translocation and activation of Hippo pathway target oncogenes in hepatocellular carcinoma. PPP1CA knockdown combined with YAP1 inhibitors synergistically suppresses HCC tumor growth in vivo. Co-immunoprecipitation, phospho-YAP western blot, nuclear fractionation, xenograft mouse model, synergy assay with YAP1 inhibitors Cellular signalling Medium 40513845
2025 PP1A (PPP1CA) inhibits ferroptosis in hepatocellular carcinoma cells by dephosphorylating Keap1 at site 104 (S104), which disrupts Keap1-Nrf2 interaction and enhances transcription of ferroptosis-related markers and immune checkpoint PD-L1. PP1A knockdown alleviates T cell exhaustion and improves the efficacy of Lenvatinib plus immune checkpoint inhibitor combination therapy. Transcriptomic analysis, ferroptosis metabolite profiling (ROS, Fe2+, lipid-ROS, GSH), PP1A knockdown, Keap1 phosphorylation western blot, single-cell sequencing, co-culture T cell assays, in vivo tumor models Advanced science Medium 40344394

Source papers

Stage 0 corpus · 101 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 PKA phosphorylation dissociates FKBP12.6 from the calcium release channel (ryanodine receptor): defective regulation in failing hearts. Cell 1641 10830164
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
1993 The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes & development 1351 8384581
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2005 Nucleolar proteome dynamics. Nature 934 15635413
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2002 Protein phosphatase 1--targeted in many directions. Journal of cell science 750 11839776
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2005 Contributions of protein phosphatases PP1, PP2A, PP2B and PP5 to the regulation of tau phosphorylation. The European journal of neuroscience 617 16262633
2002 Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel. Science (New York, N.Y.) 591 11799244
2003 BAD and glucokinase reside in a mitochondrial complex that integrates glycolysis and apoptosis. Nature 587 12931191
2005 High-throughput mapping of a dynamic signaling network in mammalian cells. Science (New York, N.Y.) 553 15761153
1984 DARPP-32, a dopamine-regulated neuronal phosphoprotein, is a potent inhibitor of protein phosphatase-1. Nature 543 6087160
2017 Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science (New York, N.Y.) 533 28302793
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2015 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell 433 26638075
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2013 Phosphorylation of FOXP3 controls regulatory T cell function and is inhibited by TNF-α in rheumatoid arthritis. Nature medicine 430 23396208
2010 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.) 421 20360068
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
1995 Protein kinase C is regulated in vivo by three functionally distinct phosphorylations. Current biology : CB 403 8749392
1996 Normalization and subtraction: two approaches to facilitate gene discovery. Genome research 401 8889548
2013 Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions. Science signaling 383 24255178
2008 PP2B and PP1alpha cooperatively disrupt 7SK snRNP to release P-TEFb for transcription in response to Ca2+ signaling. Genes & development 153 18483222
2019 USP11 promotes growth and metastasis of colorectal cancer via PPP1CA-mediated activation of ERK/MAPK signaling pathway. EBioMedicine 109 31521612
2014 Canonical Wnt signalling activates TAZ through PP1A during osteogenic differentiation. Cell death and differentiation 96 24510127
2020 Processing of the SARS-CoV pp1a/ab nsp7-10 region. The Biochemical journal 91 32083638
2010 PINCH1 regulates Akt1 activation and enhances radioresistance by inhibiting PP1alpha. The Journal of clinical investigation 85 20530873
2006 Proteolytic maturation of replicase polyprotein pp1a by the nsp4 main proteinase is essential for equine arteritis virus replication and includes internal cleavage of nsp7. The Journal of general virology 85 17098961
1999 Processing of the human coronavirus 229E replicase polyproteins by the virus-encoded 3C-like proteinase: identification of proteolytic products and cleavage sites common to pp1a and pp1ab. Journal of virology 84 9847320
1999 Inhibitory phosphorylation of PP1alpha catalytic subunit during the G(1)/S transition. The Journal of biological chemistry 81 10506210
2011 PP1A-mediated dephosphorylation positively regulates YAP2 activity. PloS one 73 21909427
2021 SRPK1/2 and PP1α exert opposite functions by modulating SRSF1-guided MKNK2 alternative splicing in colon adenocarcinoma. Journal of experimental & clinical cancer research : CR 68 33602301
2019 Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis. Nature communications 54 31332168
2008 PPP1CA contributes to the senescence program induced by oncogenic Ras. Carcinogenesis 54 18204081
2013 ERK/PP1a/PLB/SERCA2a and JNK pathways are involved in luteolin-mediated protection of rat hearts and cardiomyocytes following ischemia/reperfusion. PloS one 53 24386130
2008 RNA interference in hippocampus demonstrates opposing roles for CREB and PP1alpha in contextual and temporal long-term memory. Genes, brain, and behavior 44 19191880
2023 Arginine methylation of PPP1CA by CARM1 regulates glucose metabolism and affects osteogenic differentiation and osteoclastic differentiation. Clinical and translational medicine 42 37649137
2021 The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma. Cell death and differentiation 40 34799729
2024 A novel peptide PDHK1-241aa encoded by circPDHK1 promotes ccRCC progression via interacting with PPP1CA to inhibit AKT dephosphorylation and activate the AKT-mTOR signaling pathway. Molecular cancer 39 38360682
2018 Deregulated PP1α phosphatase activity towards MAPK activation is antagonized by a tumor suppressive failsafe mechanism. Nature communications 39 29335436
2017 Lineage A Betacoronavirus NS2 Proteins and the Homologous Torovirus Berne pp1a Carboxy-Terminal Domain Are Phosphodiesterases That Antagonize Activation of RNase L. Journal of virology 35 28003490
2017 Oxidative stress promotes SIRT1 recruitment to the GADD34/PP1α complex to activate its deacetylase function. Cell death and differentiation 35 28984870
2008 Drosophila Uri, a PP1alpha binding protein, is essential for viability, maintenance of DNA integrity and normal transcriptional activity. BMC molecular biology 33 18412953
2021 LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction. Cancer letters 31 33675985
2018 Involvement of PKA/DARPP-32/PP1α and β- arrestin/Akt/GSK-3β Signaling in Cadmium-Induced DA-D2 Receptor-Mediated Motor Dysfunctions: Protective Role of Quercetin. Scientific reports 29 29410441
2006 Identification of PP1alpha as a caspase-9 regulator in IL-2 deprivation-induced apoptosis. Journal of immunology (Baltimore, Md. : 1950) 28 16888006
2005 Protein phosphatase-1 inhibitor-3 is co-localized to the nucleoli and centrosomes with PP1gamma1 and PP1alpha, respectively. Archives of biochemistry and biophysics 28 16256067
2023 Dietary potassium stimulates Ppp1Ca-Ppp1r1a dephosphorylation of kidney NaCl cotransporter and reduces blood pressure. The Journal of clinical investigation 27 37676724
2021 Oncogenic SNORD12B activates the AKT-mTOR-4EBP1 signaling in esophageal squamous cell carcinoma via nucleus partitioning of PP-1α. Oncogene 27 33941854
2022 Comprehensive analysis of PPPCs family reveals the clinical significance of PPP1CA and PPP4C in breast cancer. Bioengineered 25 34964699
2023 Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration. Cell death and differentiation 24 37605006
2014 Sensitive biosensor based on recombinant PP1α for microcystin detection. Biosensors & bioelectronics 23 25459056
2017 Integrin-FAK-CDC42-PP1A signaling gnaws at YAP/TAZ activity to control incisor stem cells. BioEssays : news and reviews in molecular, cellular and developmental biology 22 28891248
2021 Potential 3-chymotrypsin-like cysteine protease cleavage sites in the coronavirus polyproteins pp1a and pp1ab and their possible relevance to COVID-19 vaccine and drug development. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 21 33913206
2021 PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy. Experimental eye research 21 34280391
2014 Standardised extract of Bacopa monniera (CDRI-08) improves contextual fear memory by differentially regulating the activity of histone acetylation and protein phosphatases (PP1α, PP2A) in hippocampus. Cellular and molecular neurobiology 21 24610280
2021 Downregulation of lncRNA TSLNC8 promotes melanoma resistance to BRAF inhibitor PLX4720 through binding with PP1α to re-activate MAPK signaling. Journal of cancer research and clinical oncology 20 33389075
2010 Glioblastoma-specific protein interaction network identifies PP1A and CSK21 as connecting molecules between cell cycle-associated genes. Cancer research 20 20663907
2007 Regulation of intracellular calcium release and PP1alpha in a mechanism for 4-hydroxytamoxifen-induced cytotoxicity. Molecular and cellular biochemistry 20 17646931
2019 The effect of decitabine on the expression and methylation of the PPP1CA, BTG2, and PTEN in association with changes in miR-125b, miR-17, and miR-181b in NALM6 cell line. Journal of cellular biochemistry 18 30912184
2023 CircGPRC5A enhances colorectal cancer progress by stabilizing PPP1CA and inducing YAP dephosphorylation. Journal of experimental & clinical cancer research : CR 17 38057879
2019 Dephosphorylation of the transcriptional cofactor NACA by the PP1A phosphatase enhances cJUN transcriptional activity and osteoblast differentiation. The Journal of biological chemistry 16 30948508
2015 HSV targeting of the host phosphatase PP1α is required for disseminated disease in the neonate and contributes to pathogenesis in the brain. Proceedings of the National Academy of Sciences of the United States of America 16 26621722
2011 Taperin (c9orf75), a mutated gene in nonsyndromic deafness, encodes a vertebrate specific, nuclear localized protein phosphatase one alpha (PP1α) docking protein. Biology open 16 23213405
2019 MIIP inhibits the growth of prostate cancer via interaction with PP1α and negative modulation of AKT signaling. Cell communication and signaling : CCS 13 31092266
1993 Selective high expression of protein phosphatase pp1-alpha messenger-RNA in rat poorly differentiated ascites hepatomas. International journal of oncology 13 21573543
2014 Development and validation of a robust and sensitive assay for the discovery of selective inhibitors for serine/threonine protein phosphatases PP1α (PPP1C) and PP5 (PPP5C). Assay and drug development technologies 12 25383722
2014 14-3-3ζ regulates nuclear trafficking of protein phosphatase 1α (PP1α) in HEK-293 cells. Archives of biochemistry and biophysics 11 24956593
1999 Up-regulation of nuclear PP1alpha and PP1delta in hepatoma cells. International journal of oncology 11 9863017
2013 PP1α, PP1β and Wip-1 regulate H4S47 phosphorylation and deposition of histone H3 variant H3.3. Nucleic acids research 10 23828041
2023 KIF18A interacts with PPP1CA to promote the malignant development of glioblastoma. Experimental and therapeutic medicine 9 36911368
2018 The reduced activity of PP-1α under redox stress condition is a consequence of GSH-mediated transient disulfide formation. Scientific reports 9 30531830
2024 METTL14-mediated upregulation of lncRNA HOTAIR represses PP1α expression by promoting H3K4me1 demethylation in oxycodone-treated mice. CNS neuroscience & therapeutics 8 39046182
2022 PP1A prevents ROS-induced pyroptosis by inhibiting MAPK/caspase-3 in mouse adipose tissue. The FEBS journal 8 35080339
2016 An HSV-based library screen identifies PP1α as a negative TRPV1 regulator with analgesic activity in models of pain. Molecular therapy. Methods & clinical development 8 27382601
1995 Comparative mapping of the gene encoding the catalytic subunit of protein phosphatase type 1 alpha (PPP1CA) to human, rat, and mouse chromosomes. Cytogenetics and cell genetics 8 7736790
2024 The NS2B-PP1α-eIF2α axis: Inhibiting stress granule formation and Boosting Zika virus replication. PLoS pathogens 7 38935808
2023 E2F1-mediated ectopic expression of PP1A promotes breast cancer progression via activation of YAP1. The international journal of biochemistry & cell biology 6 36787863
2022 Expression and methylation status of BTG2, PPP1CA, and PEG3 genes in colon adenocarcinoma cell lines: promising treatment targets. Gastroenterology and hepatology from bed to bench 4 36762213
1997 Alterations in type-1 serine/threonine protein phosphatase PP1alpha in response to B-cell receptor stimulation. Journal of biochemistry 4 9399575
2025 PP1A Modulates the Efficacy of Lenvatinib Plus ICIs Therapy by Inhibiting Ferroptosis in Hepatocellular Carcinoma. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 3 40344394
2013 PP-1α and PP-1γ display antagonism and differential roles in tumorigenicity of lung cancer cells. Current molecular medicine 2 23176181
2025 PPP1CA promotes hepatocellular carcinoma progression in a YAP1-dependent way. Cellular signalling 1 40513845
2025 NY-ESO-1 facilitates anoikis resistance and tumor metastasis by hijacking deubiquitinase OTUB1 to stabilize PP1α. Cell death & disease 1 41053024
2025 Exosomal miR-212-5p promotes tendon repair via targeting FOXO1 to activate PP1A/YAP1 signaling. Communications biology 1 41326793
2024 In-silico analysis of potential anticancer drug for NEK7 and PPP1CA proteins overexpressed in pancreatic ductal adenocarcinoma. Journal of biomolecular structure & dynamics 1 38469816
2024 The epigenetically regulated PP1α expression by KDM1A may contribute to oxycodone conditioned place preference in mice. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 1 38870630
2024 S100A4 targets PPP1CA/IL-17 to inhibit the senescence of sheep endometrial epithelial cells. Frontiers in veterinary science 1 39664902
2023 Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9. Stem cell research 1 36965407
2002 Further in vitro characterization of mouse hepatitis virus papain-like proteinase 1: cleavage sequence requirements within pp1a. Journal of neurovirology 1 11935466
2026 IIIG9 and PP1α form a protein complex in the adherens junctions of polarized ependymal and MDCK cells. Fluids and barriers of the CNS 0 41566508
2026 ELFN2 inhibits YAP-driven lymph node metastasis in gastric cancer by blocking PP1A-mediated dephosphorylation. Experimental cell research 0 41967793
2024 Nodularin-R Synergistically Enhances Abiraterone Against Castrate- Resistant Prostate Cancer via PPP1CA Inhibition. Journal of cellular and molecular medicine 0 39550701