Affinage

PPP1CA

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit · UniProt P62136

Length
330 aa
Mass
37.5 kDa
Annotated
2026-06-10
71 papers in source corpus 34 papers cited in narrative 34 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 5/5 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PPP1CA (PP1α) is a broadly acting serine/threonine phosphatase whose substrate selection, subcellular localization, and activity are dictated by interchangeable regulatory/targeting subunits and a layered code of post-translational modifications, allowing one catalytic core to control transcription, cell-cycle progression, survival signaling, the translational stress response, and ion transport (PMID:18483222, PMID:37676724, PMID:28984870). Its substrate repertoire is dictated by partner-directed targeting: it dephosphorylates Cdk9-Thr186 to release active P-TEFb from the 7SK snRNP downstream of Ca2+/PP2B signaling (PMID:18483222), caspase-9-Thr125 to license apoptosis upon IL-2 withdrawal (PMID:16888006), YAP-Ser127 and TAZ to drive Hippo-effector nuclear accumulation (PMID:21909427, PMID:24510127), PAK1-Thr423 within a Smad4–MYO18A–PP1A holoenzyme (PMID:34799729), the NaCl cotransporter NCC in a potassium-regulated manner to modulate blood pressure (PMID:37676724), NACA to potentiate AP-1 transcription (PMID:30948508), and eIF2α (via the GADD34/PP1α complex) to restore translation after oxidative stress (PMID:28984870). Activity and access to substrates are gated by inhibitory phosphorylation at Thr320 by Cdk2 across cell-cycle transitions (PMID:10506210) and at Ser325 by PKC during B-cell receptor signaling (PMID:9399575), by redox-driven intramolecular disulfide formation protected by glutathione (PMID:30531830), and by CARM1-mediated arginine-23 methylation and SUV39H2-mediated lysine-141 methylation, the latter disrupting PP1α–TFEB and PP1α–spinophilin engagement to impair autophagy (PMID:37649137, PMID:37605006). Nucleocytoplasmic partitioning is set by competing interactors—14-3-3ζ enforces cytoplasmic retention while diverse RNAs, peptides, and viral/oncogenic proteins (SNORD12B, PDHK1-241aa, PML) relocalize the enzyme (PMID:24956593, PMID:33941854, PMID:38360682, PMID:29335436)—and its abundance is stabilized by the deubiquitinases USP11 and OTUB1, which feed ERK/MAPK-driven tumor progression (PMID:31521612, PMID:41053024). PP1α functions as a regulatory node in oncogenic Ras-induced senescence, requiring PPP1CA for p53/p21-dependent pRb dephosphorylation (PMID:18204081), and is hijacked by HSV-1 (γ34.5) and Zika virus (NS2B) to dephosphorylate eIF2α and overcome translational arrest during infection (PMID:26621722, PMID:38935808).

Mechanistic history

Synthesis pass · year-by-year structured walk · 31 steps
  1. 1997 Medium

    Established that PP1α activity is regulated by signal-dependent C-terminal phosphorylation, identifying PKC-Ser325 as an isoform-specific modification linking BCR signaling to transient phosphatase suppression.

    Evidence In vitro PKC phosphorylation of C-terminal peptides, 2D electrophoresis, PP1 activity assays in a B-cell stimulation model

    PMID:9399575

    Open questions at the time
    • Downstream substrate consequences of Ser325 phosphorylation not defined
    • Physiological PP1α targets in BCR signaling not mapped
  2. 1999 High

    Defined Cdk2-mediated Thr320 phosphorylation as a cell-cycle switch creating distinct PP1α subpopulations governing G1/S versus M-phase transitions.

    Evidence In vitro kinase assay with Cdk2/Cdk4, phospho-specific antibodies, co-IP with pRB, T320A mutagenesis, cell-cycle synchronization

    PMID:10506210

    Open questions at the time
    • Direct cell-cycle substrates dephosphorylated by the Thr320-controlled pool not enumerated
    • Phosphatase that reverses Thr320 not identified
  3. 2005 Medium

    Demonstrated isoform-selective targeting of PP1α by inhibitor-3 to centrosomes, an early example of regulatory-subunit-driven spatial control distinguishing PP1α from PP1β/γ.

    Evidence Immunofluorescence co-localization, co-IP, mutagenesis of nuclear/nucleolar targeting signals in HEK293 cells

    PMID:16256067

    Open questions at the time
    • Functional consequence of centrosomal PP1α localization unresolved
    • Centrosomal substrates not identified
  4. 2006 High

    Linked PP1α to apoptotic commitment by showing it directly dephosphorylates caspase-9-Thr125 upon survival-signal withdrawal, with novel non-RVxF binding sites on the substrate.

    Evidence In vivo/in vitro co-IP, cell-free caspase activation reconstitution, siRNA knockdown in an IL-2 deprivation model

    PMID:16888006

    Open questions at the time
    • Regulatory subunit directing PP1α to caspase-9 not identified
    • Upstream signal activating PP1α after IL-2 withdrawal not fully resolved
  5. 2008 High

    Placed PP1α as the effector phosphatase that releases active P-TEFb from the 7SK snRNP, connecting Ca2+/PP2B signaling to transcriptional elongation via Cdk9-Thr186 dephosphorylation.

    Evidence Co-IP, in vitro phosphatase assays on Cdk9 T186, siRNA knockdown, UV/HMBA stimulation

    PMID:18483222

    Open questions at the time
    • Targeting subunit recruiting PP1α to 7SK snRNP not defined
    • How PP2B-induced conformational change grants PP1α access at structural level unresolved
  6. 2008 High

    Established PP1α as a positive effector of oncogene-induced senescence, required for p53/p21-driven pRb dephosphorylation and ceramide-mediated growth arrest.

    Evidence Retroviral genetic screen, shRNA knockdown, p21/pRb phospho-blotting, ceramide addition, tumor growth assays

    PMID:18204081

    Open questions at the time
    • Direct vs indirect role of PP1α in pRb dephosphorylation not separated
    • Mechanism linking ceramide to PP1α activation unclear
  7. 2008 Medium

    Demonstrated conserved isoform-discriminating binding of URI for PP1α over PP1β, reinforcing that PP1α partner selectivity has functional consequences for transcription and genome stability.

    Evidence Binding affinity measurements (Drosophila and human URI), uri loss-of-function allele, viability/transcription/DNA-damage assays

    PMID:18412953

    Open questions at the time
    • Human URI–PP1α functional consequences not directly tested
    • Substrates of the URI–PP1α complex not defined
  8. 2011 High

    Identified PP1α as a Hippo-pathway phosphatase dephosphorylating YAP-Ser127 to drive nuclear accumulation and transcriptional activation, opposing cytoplasmic sequestration.

    Evidence Co-IP, in vitro dephosphorylation, subcellular fractionation, okadaic acid treatment, reporter assays, siRNA knockdown

    PMID:21909427

    Open questions at the time
    • Regulatory subunit targeting PP1α to YAP not identified
    • Relationship to LATS kinase signaling not delineated here
  9. 2010 High

    Defined PP1α as an Akt1-dephosphorylating phosphatase at integrin adhesions whose activity is restrained by direct binding of PINCH1, coupling adhesion signaling to survival/radioresistance.

    Evidence Direct binding assay, PP1α activity assays, Akt1 phospho-measurement, siRNA knockdown, in vivo irradiation survival

    PMID:20530873

    Open questions at the time
    • Akt residue dephosphorylated by PP1α not specified
    • Generalizability beyond adhesion-site context untested
  10. 2013 Medium

    Implicated PP1α in chromatin regulation by showing it binds H3-H4 and the Pak2 kinase and controls histone H4S47 phosphorylation and H3.3 deposition.

    Evidence siRNA knockdown, co-IP for H3-H4 and Pak2, ChIP for H3.3 occupancy, H4S47ph blotting

    PMID:23828041

    Open questions at the time
    • Direct dephosphorylation of H4S47 by PP1α not isolated from Pak2 regulation
    • Targeting subunit for chromatin localization unknown
  11. 2014 High

    Extended PP1α's Hippo-effector role to TAZ during Wnt3a signaling, showing dephosphorylation blocks 14-3-3 binding, stabilizes TAZ, and drives osteoblastic differentiation.

    Evidence siRNA knockdown, TAZ phospho-blotting, 14-3-3 co-IP, nuclear fractionation, osteogenic differentiation assays

    PMID:24510127

    Open questions at the time
    • Targeting subunit recruiting PP1α to TAZ not identified
    • Crosstalk with the YAP arm not resolved
  12. 2014 Medium

    Established 14-3-3ζ as a phosphorylation-independent cytoplasmic retention factor for PP1α, defining a key node in PP1α nucleocytoplasmic partitioning without affecting catalytic activity.

    Evidence Co-IP, GST pulldown, deletion mapping (residues 159-279), in vitro activity assay, subcellular fractionation, dominant-negative 14-3-3ζ (K49E)

    PMID:24956593

    Open questions at the time
    • Signals that release PP1α from 14-3-3ζ not defined here
    • Substrate consequences of localization shift not measured
  13. 2017 Medium

    Revealed redox control of PP1α via reversible intramolecular disulfide formation (Cys39/Cys127) that, under glutathione, protects the enzyme from irreversible over-oxidation while lowering activity.

    Evidence Disulfide-targeted mass spectrometry, in vitro activity assays under oxidative conditions, site-specific cysteine analysis

    PMID:30531830

    Open questions at the time
    • Physiological redox conditions triggering this in cells not established
    • Substrate-level consequences not tested
  14. 2017 High

    Showed the GADD34/PP1α complex acts on dual stress substrates—eIF2α-pSer51 and SIRT1-pSer47—coupling translational recovery to SIRT1 deacetylase activation after oxidative stress.

    Evidence GADD34 acetylation MS, co-IP of GADD34/PP1α/eIF2α/SIRT1, rescue in GADD34-/- MEFs with PP1α-binding-deficient mutant, deacetylase activity assays

    PMID:28984870

    Open questions at the time
    • Whether SIRT1 is a direct PP1α substrate vs GADD34-bridged not fully separated
    • Selectivity over CReP-targeted PP1α not addressed
  15. 2015 High

    Demonstrated that HSV-1 γ34.5 hijacks PP1α to dephosphorylate eIF2α and reverse translational arrest, with PP1α-binding required for disseminated neonatal disease in vivo.

    Evidence Neonatal murine infection, PP1α-binding mutant virus, type I IFN-deficient rescue, brain-slice pharmacologic inhibition

    PMID:26621722

    Open questions at the time
    • Structural basis of γ34.5–PP1α recruitment not detailed
    • Host eIF2α kinase counteracted not specified in this work
  16. 2016 Medium

    Identified PP1α as a negative regulator of TRPV1 thermal channel activity, expanding its role to sensory signaling.

    Evidence HSV-based cDNA screen, viral replication rescue, in vivo rat thermal sensitivity assays

    PMID:27382601

    Open questions at the time
    • Biochemical mechanism of TRPV1 regulation (direct dephosphorylation?) not defined
    • TRPV1 phosphosite not identified
  17. 2019 High

    Mapped a multi-site PP1α–NACA dephosphorylation event (Thr89, Ser151, Thr174) through a defined holoenzyme (PPP1R9B/R12A/R18) that drives NACA nuclear localization and AP-1 transcriptional potentiation.

    Evidence AP-MS of holoenzyme, co-IP, phosphosite MS, phosphomimetic/alanine mutants, AP-1 reporter and osteogenic assays

    PMID:30948508

    Open questions at the time
    • Which specific regulatory subunit targets NACA in vivo not isolated
    • Kinase counteracted on NACA not defined
  18. 2019 Medium

    Defined deubiquitinase-mediated stabilization of PP1α by USP11, linking PP1α abundance to ERK/MAPK-driven colorectal cancer progression.

    Evidence LC-MS/MS interactome, co-IP, ubiquitination assays, proteasome inhibition, in vitro/in vivo tumor assays

    PMID:31521612

    Open questions at the time
    • Ubiquitin ligase targeting PP1α not identified
    • How ERK activation links to PP1α catalytic substrates unresolved
  19. 2019 High

    Showed Cavin-3, released from caveolae upon UV stress, binds and inhibits PP1α to promote H2AX phosphorylation and apoptosis, defining a surface-to-nucleus pro-apoptotic relay.

    Evidence Biotin affinity proteomics, MS, cell-free binding, co-IP, UV stress, H2AX phospho-measurement

    PMID:31332168

    Open questions at the time
    • Whether H2AX is a direct PP1α substrate not established
    • Quantitative contribution of PP1α inhibition to apoptosis not isolated
  20. 2019 Medium

    Established MIIP as a positive regulator that enhances PP1α-mediated Akt dephosphorylation, with its tumor-suppressive function dependent on the PP1α-interacting C-terminus.

    Evidence Co-IP, co-localization, Akt phospho-blotting, PP1α siRNA epistasis, MIIPΔC mutants, xenografts

    PMID:31092266

    Open questions at the time
    • Akt residue targeted not specified
    • Direct vs scaffolding role of MIIP not resolved
  21. 2021 Medium

    Identified PP1α as a regulator of SRSF1 phosphorylation/localization opposing SRPK1/2, controlling MKNK2 alternative splicing in colon cancer.

    Evidence PP1α activity assays, siRNA knockdown, splicing PCR, IHC, xenografts

    PMID:33602301

    Open questions at the time
    • Direct SRSF1 dephosphorylation site by PP1α not mapped
    • Targeting subunit for SRSF1 not identified
  22. 2021 High

    Reconstituted a Smad4–MYO18A–PP1A holoenzyme in which MYO18A serves as the RVFFR-motif regulatory subunit directing PP1A to dephosphorylate PAK1-Thr423 and suppress β-catenin nuclear translocation.

    Evidence LC-MS/MS, co-IP, complex reconstitution, RVFFR mutagenesis, PAK1/β-catenin phospho-assays, in vivo functional assays

    PMID:34799729

    Open questions at the time
    • Generalizability of the ternary complex beyond cholangiocarcinoma untested
    • Structural detail of substrate presentation absent
  23. 2021 Medium

    Showed competing interactors reposition PP1α: LACTB displaces PP1A from YAP to increase phospho-YAP, while the noncoding RNA SNORD12B disrupts the 14-3-3ζ–PP1α interaction to drive nuclear relocalization and cytosolic AKT-mTOR activation.

    Evidence Co-IP, subcellular fractionation, YAP/AKT phospho-blotting, RNAi/overexpression, xenografts

    PMID:33675985 PMID:33941854

    Open questions at the time
    • Quantitative kinetics of competitive displacement not measured
    • Direct vs indirect AKT regulation upon relocalization not separated
  24. 2023 Medium

    Demonstrated arginine methylation of PP1α at R23 by CARM1 reprograms substrate output toward AKT-T450/AMPK-T172 dephosphorylation and glycolytic flux, controlling bone-cell differentiation.

    Evidence In vitro methylation assays, CRISPR KO, lentiviral OE, transcriptomic/metabolomic profiling, AKT/AMPK phospho-blotting

    PMID:37649137

    Open questions at the time
    • Mechanism by which R23 methylation alters substrate selectivity unresolved
    • Whether methylation alters partner binding not tested
  25. 2023 High

    Established lysine methylation of PP1α at K141 by SUV39H2 as an inhibitory mark that disrupts PP1α–TFEB and PP1α–spinophilin (PPP1R9B) engagement, blocking TFEB dephosphorylation, autophagy, and accelerating senescence.

    Evidence Proteomics, in vitro methylation, co-IP disruption, TFEB phospho/localization assays, CRISPR KO/OE, autophagy assays, IDD animal models

    PMID:37605006

    Open questions at the time
    • Demethylase reversing K141 not identified
    • Structural basis for K141-dependent partner discrimination unresolved
  26. 2023 High

    Defined PP1A as the potassium-regulated NCC phosphatase in kidney, directly dephosphorylating NCC with its negative regulator I1 (PPP1R1A) suppressed by dietary potassium, modulating blood pressure.

    Evidence Transcriptomics, direct binding assay, in vivo dephosphorylation in SPAK-constitutively-active mice, high-K diet, blood pressure measurement

    PMID:37676724

    Open questions at the time
    • Targeting subunit directing PP1A to NCC not fully defined
    • Relative contribution vs PP3/calcineurin not isolated
  27. 2024 Medium

    Showed the circPDHK1-encoded peptide PDHK1-241aa relocalizes PPP1CA to the nucleus to relieve cytoplasmic AKT dephosphorylation and activate AKT-mTOR in renal cell carcinoma, a second relocalization-based hijack mechanism.

    Evidence IP-MS, co-IP, immunofluorescence, fractionation, AKT phospho-blotting, ccRCC cell and nude-mouse assays

    PMID:38360682

    Open questions at the time
    • AKT residue and direct substrate status not specified
    • Competition with 14-3-3ζ for PP1α not examined
  28. 2024 High

    Demonstrated Zika NS2B scaffolds PP1α onto eIF2α to promote dephosphorylation, suppress stress granules, and stabilize PP1α against degradation, mirroring viral subversion of the translational stress response.

    Evidence Co-IP of NS2B-PP1α-eIF2α complex, eIF2α dephosphorylation and stress-granule assays, NS2BV35A mutagenesis, ubiquitination-resistance and viral replication assays, brain organoids

    PMID:38935808

    Open questions at the time
    • Whether NS2B alters PP1α catalytic activity vs only localization unresolved
    • Host factors competing for PP1α during infection not mapped
  29. 2025 Medium

    Identified OTUB1 (recruited by NY-ESO-1) as a second PP1α-stabilizing deubiquitinase whose catalytic, not E2-suppressing, activity prevents PP1α polyubiquitination and drives ERK1/2-dependent metastasis.

    Evidence Ternary-complex co-IP, ubiquitination/deubiquitination assays, PP1α knockdown rescue, ERK1/2 activity, in vivo metastasis assays

    PMID:41053024

    Open questions at the time
    • Ubiquitin ligase opposing OTUB1 on PP1α not identified
    • Link between PP1α accumulation and ERK substrates not mechanistically detailed
  30. 2025 Medium

    Showed PP1A dephosphorylates Keap1-Ser104 to disrupt Keap1-Nrf2, upregulating ferroptosis markers and PD-L1 and limiting Lenvatinib-induced ferroptosis in hepatocellular carcinoma.

    Evidence Transcriptomics, ferroptosis metabolite profiling, PP1A knockdown, Keap1-Nrf2 co-IP, PD-L1 blotting, single-cell sequencing, T-cell exhaustion co-culture, in vivo models

    PMID:40344394

    Open questions at the time
    • Targeting subunit recruiting PP1A to Keap1 not defined
    • Direct dephosphorylation of Keap1 vs indirect effect not fully isolated
  31. 2025 Medium

    Provided site-resolved kinetics showing PP1α asymmetrically removes AMPK activation-loop phosphorylation while sparing autophosphorylation sites, refining how PP1α shapes AMPK regulatory states.

    Evidence In vitro phosphatase competition assay with integrated bottom-up/top-down MS proteoform analysis (preprint)

    PMID:bio_10.1101_2025.10.10.681638

    Open questions at the time
    • Not yet peer-reviewed
    • Cellular relevance of site selectivity not validated
    • Regulatory subunit directing AMPK dephosphorylation unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the combinatorial PTM code (Thr320/Ser325 phosphorylation, R23/K141 methylation, Cys oxidation) is integrated with the >100 possible regulatory subunits to specify substrate choice and localization in any given cell remains unresolved.
  • No structural model linking PP1α PTM state to subunit selectivity
  • Quantitative subunit occupancy in vivo not measured
  • Reciprocal regulation between localization controllers (14-3-3ζ, PML, RNAs) not unified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 10 GO:0016787 hydrolase activity 6 GO:0042393 histone binding 1
Localization
GO:0005634 nucleus 4 GO:0005829 cytosol 3 GO:0005815 microtubule organizing center 1
Pathway
R-HSA-162582 Signal Transduction 5 R-HSA-392499 Metabolism of proteins 3 R-HSA-1640170 Cell Cycle 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-74160 Gene expression (Transcription) 2 R-HSA-8953897 Cellular responses to stimuli 2
Partners
14-3-3ΖGADD34MYO18ANACAOTUB1PINCH1PPP1R9BUSP11
Complex memberships
7SK snRNPGADD34/PP1α complexNY-ESO-1/OTUB1/PP1α complexSmad4-MYO18A-PP1A complex

Evidence

Reading pass · 34 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2008 PP1α (PPP1CA) dephosphorylates phospho-Thr186 in the Cdk9 T-loop, thereby releasing P-TEFb from the inactive 7SK snRNP complex. This occurs downstream of Ca2+/PP2B signaling: PP2B first induces a conformational change in 7SK snRNP that enables PP1α access, and PP1α then dephosphorylates Cdk9 Thr186. The released, dephosphorylated P-TEFb is subsequently recruited by Brd4 to the preinitiation complex. Co-immunoprecipitation, in vitro phosphatase assays, siRNA knockdown, UV/HMBA stimulation of P-TEFb release Genes & development High 18483222
1999 PP1α (PPP1CA) is phosphorylated at Thr-320 by Cdk2 (but not Cdk4) during M-phase and again at the G1/S transition in a pRB-dependent manner. PP1α phosphorylated at Thr-320 co-precipitates with pRB during G1/S but is found in pRB-immunodepleted extracts in M-phase, indicating distinct subpopulations of PP1α regulate different cell cycle transitions. Expression of a T320A phosphorylation-resistant PP1α mutant in pRB-expressing cells causes G1 arrest. In vitro kinase assay with Cdk2/Cdk4, phospho-specific antibodies, co-immunoprecipitation with pRB, inhibitor treatments (Cdk2 inhibitor), cell cycle synchronization The Journal of biological chemistry High 10506210
2006 PP1α (PPP1CA) associates with caspase-9 in vitro and in vivo in IL-2-proliferating cells. IL-2 deprivation induces PP1α dephosphorylation and activation, leading to dephosphorylation of phospho-Thr125 on caspase-9, thereby activating caspase-9 and subsequent caspase-3 cleavage. PP1α is indispensable for triggering caspase-9 in cell-free systems. Two novel PP1α-binding sites on caspase-9 distinct from the canonical RVxF motif were identified. Co-immunoprecipitation in vivo and in vitro, cell-free caspase activation assays, siRNA knockdown of PP1α, IL-2 deprivation model Journal of immunology High 16888006
2010 PINCH1 directly binds PP1α and inhibits its phosphatase activity, resulting in increased Akt1 phosphorylation and enhanced cell survival/radioresistance. PP1α acts as an Akt1-dephosphorylating phosphatase that is negatively regulated by PINCH1 at integrin adhesion sites. Direct binding assay (PINCH1-PP1α interaction), PP1α activity assays, Akt1 phosphorylation measurements, siRNA knockdown, in vitro and in vivo irradiation survival assays The Journal of clinical investigation High 20530873
2014 PP1A (PPP1CA) dephosphorylates TAZ during canonical Wnt3a signaling. Wnt3a-induced dephosphorylation of TAZ by PP1A prevents TAZ from binding 14-3-3 proteins, stabilizes TAZ, and induces its nuclear localization, thereby promoting osteoblastic differentiation. Depletion of PP1A blocks Wnt3a-induced TAZ stabilization. siRNA knockdown of PP1A, western blotting for TAZ phosphorylation, co-immunoprecipitation with 14-3-3, nuclear fractionation, osteogenic differentiation assays Cell death and differentiation High 24510127
2011 PP1A (PPP1CA) interacts with and dephosphorylates YAP2 at Serine-127 in vitro and in vivo. PP1A-mediated YAP2 dephosphorylation induces nuclear accumulation and transcriptional activation of YAP2. Inhibition of PP1 by okadaic acid increases YAP2 phospho-Ser127 and promotes its cytoplasmic retention. Co-immunoprecipitation, in vitro dephosphorylation assay, subcellular fractionation, okadaic acid treatment, YAP2 transcriptional reporter assays, siRNA knockdown PloS one High 21909427
2008 PPP1CA (PP1α) contributes to oncogenic Ras-induced senescence. Loss of PPP1CA function (via antisense RNA or shRNA) bypasses ras/p53-induced growth arrest and senescence. PPP1CA is required for p53-dependent induction of p21 after DNA damage and subsequent pRb dephosphorylation. Oncogenic Ras increases intracellular ceramide levels and PPP1CA protein levels; ceramide-induced senescence is blocked by PPP1CA downregulation. Retroviral genetic screen, shRNA knockdown of PPP1CA, western blotting for p21 and pRb phosphorylation, ceramide addition experiments, tumor growth assays Carcinogenesis High 18204081
2018 PP1α (PPP1CA) acts as a B-Raf activating phosphatase in an S6K/PP1α/B-Raf signaling pathway that leads to MAPK activation. PML sequesters PP1α into PML nuclear bodies, repressing S6K-dependent PP1α phosphorylation, 14-3-3 binding, and cytoplasmic accumulation. PPP1CA gene is genomically amplified in metastatic prostate cancer. Co-immunoprecipitation, subcellular fractionation, in vitro phosphatase assays, genomic amplification analysis, PML knockdown/overexpression, B-Raf activity assays Nature communications High 29335436
2019 USP11 stabilizes PPP1CA by deubiquitinating it, protecting PPP1CA from proteasome-mediated degradation. The USP11/PPP1CA complex activates the ERK/MAPK signaling pathway to promote colorectal cancer progression. LC-MS/MS interactome, co-immunoprecipitation, ubiquitination assays, proteasome inhibitor treatment, siRNA/overexpression experiments, in vitro and in vivo tumor assays EBioMedicine Medium 31521612
2019 Cavin-3, upon UV-induced release from caveolae, interacts with and inhibits PP1α. This interaction increases H2AX phosphorylation to stimulate apoptosis, defining a pro-apoptotic signaling pathway from surface caveolae to the nucleus. Biotin affinity proteomics, mass spectrometry, cell-free protein binding assays, co-immunoprecipitation, UV stress experiments, H2AX phosphorylation measurement Nature communications High 31332168
2021 PP1A (PPP1CA) dephosphorylates SRSF1, and reduced PP1α activity enhances SRSF1 phosphorylation and nucleus translocation, causing a switch in MKNK2 alternative splicing from MKNK2a to MKNK2b in colon adenocarcinoma. PP1α and SRPK1/2 exert opposing functions balancing SRSF1 phosphorylation and localization. Western blotting, immunohistochemistry, PP1α activity assays, siRNA knockdown, alternative splicing analysis by PCR, xenograft models Journal of experimental & clinical cancer research Medium 33602301
2021 The Smad4-MYO18A-PP1A complex dephosphorylates PAK1 at Thr-423. MYO18A functions as the PP1-interacting protein (regulatory subunit) for PP1A substrate recognition in cholangiocarcinoma, binding PP1A via its RVFFR motif and Smad4 via its CC domain. PP1A-mediated PAK1-T423 dephosphorylation inhibits β-catenin-S675 phosphorylation and its nuclear translocation. LC-MS/MS, co-immunoprecipitation, biochemical complex reconstitution, site-directed mutagenesis (RVFFR motif), phosphorylation assays for PAK1 and β-catenin, in vitro and in vivo functional assays Cell death and differentiation High 34799729
2023 CARM1 methylates PPP1CA at Arg-23 (R23). This arginine methylation affects PPP1CA-mediated dephosphorylation of AKT-T450 and AMPK-T172, and increases activities of phosphofructokinase-1 and PFKFB3, upregulating glycolytic flux. This mechanism promotes osteogenic differentiation and inhibits osteoclastic differentiation. In vitro methylation assays (identifying R23 as methylation site), CRISPR/Cas9 knockout, lentiviral overexpression, transcriptomic and metabolomic assays, western blotting for AKT and AMPK phosphorylation Clinical and translational medicine Medium 37649137
2023 SUV39H2 methyltransferase monomethylates PPP1CA at Lys-141 (K141). K141 methylation disrupts PPP1CA's interaction with TFEB and blocks TFEB dephosphorylation and nuclear translocation, leading to autophagy deficiency and nucleus pulposus cell senescence. PPP1R9B (spinophilin) facilitates PPP1CA-TFEB targeting, and K141 methylation also impairs PPP1R9B binding to PPP1CA. Proteomic analysis (identifying SUV39H2 as writer), in vitro methylation assays, co-immunoprecipitation, western blotting for TFEB phosphorylation and localization, CRISPR/KO and overexpression, autophagy assays, animal models of IDD Cell death and differentiation High 37605006
2023 PP1A (PPP1CA/Ppp1Ca) directly binds to and dephosphorylates the NaCl cotransporter (NCC) in the kidney in a potassium-regulated manner, reducing blood pressure. Dietary potassium upregulates Ppp1Ca expression and suppresses its negative regulator Ppp1r1a (I1), shifting the balance toward PP1A-mediated NCC dephosphorylation. Confirmed using genetically engineered mice with constitutively active SPAK kinase. Transcriptomics screen, direct binding assay (PP1A-NCC), in vivo dephosphorylation experiments in SPAK-constitutively active transgenic mice, high-potassium dietary intervention, blood pressure measurements The Journal of clinical investigation High 37676724
2008 Drosophila URI binds PP1alpha with much higher affinity than PP1beta, and this isoform-discriminating ability is conserved in humans. URI loss-of-function in Drosophila causes transcriptional defects, reduced cell viability, germline differentiation defects, and nuclear DNA damage accumulation. Binding affinity measurements (Drosophila and human URI vs PP1alpha/PP1beta), generation of uri loss-of-function allele, viability assays, transcriptional analysis, DNA damage markers BMC molecular biology Medium 18412953
2017 PP1α activity is regulated by oxidative stress via formation of transient intramolecular disulfide bonds (involving Cys39 and Cys127), which protect the enzyme from irreversible over-oxidation. Glutathione (GSH) mediates this protection mechanism, reducing PP1α activity under redox stress. The dimerization of PP1α via these cysteines protects the active surface. Mass spectrometry with cross-over-read search for disulfide-linked species, in vitro PP1α activity assays under oxidative conditions, site-specific cysteine analysis Scientific reports Medium 30531830
2013 PP1α (PPP1CA), PP1β, and Wip1 phosphatases regulate histone H4 Ser47 phosphorylation (H4S47ph) levels and H3.3 deposition. Depletion of each phosphatase increases H4S47ph. PP1α and PP1β bind H3-H4 in vitro and in vivo and also interact with the Pak2 kinase in vivo, suggesting regulation at the level of both the substrate H4S47 and the kinase Pak2. Depletion of these phosphatases increases H3.3 occupancy and chaperone (HIRA/Daxx) association. siRNA knockdown of PP1α, PP1β, and Wip1; co-immunoprecipitation for H3-H4 and Pak2 binding; ChIP for H3.3 occupancy; western blotting for H4S47ph levels Nucleic acids research Medium 23828041
2017 The GADD34/PP1α complex dephosphorylates both eIF2α (phosphoserine-51) and SIRT1 (phosphoserine-47) in response to arsenite-induced oxidative stress. A GADD34 mutant unable to bind PP1α fails to restore eIF2α or SIRT1 dephosphorylation in GADD34-/- MEFs. SIRT1 dephosphorylation by the GADD34/PP1α complex increases SIRT1 deacetylase activity, affecting cell fate after oxidative stress. Mass spectrometry of GADD34 acetylation, co-immunoprecipitation of GADD34/PP1α/eIF2α/SIRT1 complex, rescue experiments in GADD34-/- MEFs with WT vs PP1α-binding-deficient GADD34 mutant, in vitro and in-cell SIRT1 deacetylase activity assays Cell death and differentiation High 28984870
2005 PP1 inhibitor-3 (Inh3) co-localizes with PP1alpha at centrosomes (and with PP1gamma1 at nucleoli) in interphase HEK 293 cells. Inh3 co-immunoprecipitates with PP1alpha and PP1gamma1 but not PP1beta in vitro, demonstrating isoform-specific interactions. The nuclear localization signal and nucleolar targeting signals of Inh3 were mapped by site-directed mutagenesis. Immunofluorescence co-localization, co-immunoprecipitation, site-directed mutagenesis of nuclear/nucleolar localization signals, laser scanning cytometry Archives of biochemistry and biophysics Medium 16256067
2014 14-3-3ζ binds PP1α within residues 159–279 in a phosphorylation-independent manner. 14-3-3ζ does not affect PP1α catalytic activity in vitro, but causes cytoplasmic retention of PP1α: cells expressing both PP1α and 14-3-3ζ have a significantly higher cytoplasmic/nuclear PP1α ratio. Expression of dominant-negative 14-3-3ζ (K49E) causes nuclear accumulation of PP1α. Co-immunoprecipitation from HEK-293 lysates, GST pulldown, PP1α deletion mutant mapping, in vitro phosphatase activity assay, subcellular fractionation, dominant-negative 14-3-3ζ expression Archives of biochemistry and biophysics Medium 24956593
2015 HSV-1 γ34.5 protein targets PP1α to reverse translational arrest (dephosphorylation of eIF2α) during viral infection. HSV-1 carrying a PP1α-binding mutation in γ34.5 shows complete absence of disseminated disease in neonatal mice and increased survival in brain infection models. PP1α targeting by HSV-1 is required for later stages of disseminated infection but not for early replication in visceral organs. Murine neonatal infection model, HSV-1 PP1α-binding mutant virus, type I IFN-deficient mouse rescue experiments, brain slice culture pharmacologic inhibition of eIF2α dephosphorylation Proceedings of the National Academy of Sciences of the United States of America High 26621722
2019 PP1A (PPP1CA) directly dephosphorylates NACA (nascent polypeptide-associated complex and co-regulator α) at Thr-89, Ser-151, and Thr-174. NACA interacts with the PPP1CA catalytic subunit and regulatory subunits PPP1R9B, PPP1R12A, and PPP1R18. NACA dephosphorylation promotes nuclear localization of NACA, recruitment of BTF3/BTF3L4, and cooperatively potentiates cJUN/AP-1 transcriptional activity and osteoblast differentiation. Affinity purification-MS (identifying PP1A holoenzyme components), co-immunoprecipitation in HEK293T and MC3T3-E1 cells, phosphosite MS analysis, phosphomimetic and alanine substitution mutants, AP-1 reporter assays, osteogenic differentiation assays The Journal of biological chemistry High 30948508
2011 Taperin (C9orf75) preferentially docks the alpha isoform of PP1 over PP1beta via a classic RVxF motif, suppresses the general phosphatase activity of PP1α, and is found complexed to PP1α in both nuclear and cytoplasmic compartments. Taperin shuttles between nucleus and cytoplasm and is recruited to sites of DNA damage, interacting with Ku70, Ku80, PARP and topoisomerases. Biochemical binding assays, co-immunoprecipitation, SILAC MS for interactors, PP1 isoform specificity assays, subcellular fractionation, DNA damage recruitment assays Biology open Medium 23213405
2016 PP1α is a negative regulator of TRPV1 (transient receptor potential vanilloid 1) channel activity. HSV-based coexpression of PP1α rescued viral replication suppressed by TRPV1 in a cell-based screen. In vivo, HSV vectors expressing PP1α reduced thermal sensitivity in rat footpads, demonstrating functional specificity for thermal but not chemical pain modalities. HSV-based cDNA library screen for TRPV1 negative regulators, viral rescue of replication, in vivo rat thermal sensitivity assays Molecular therapy. Methods & clinical development Medium 27382601
2021 LACTB directly binds PP1A and attenuates the interaction between PP1A and YAP, reducing YAP dephosphorylation and increasing phospho-YAP (Ser127), which prevents YAP nuclear translocation in a LATS1-independent manner. This constitutes an SOX10/LACTB/PP1A signaling cascade controlling melanoma progression. Co-immunoprecipitation (LACTB-PP1A and PP1A-YAP interactions), western blotting for YAP phosphorylation, subcellular fractionation, siRNA/overexpression, in vivo xenograft experiments Cancer letters Medium 33675985
2021 SNORD12B interacts with PP1α, disrupts the interaction between 14-3-3ζ and PP1α, and causes PP1α to relocalize from the cytosol to the nucleus. Nuclear sequestration of PP1α leads to enhanced cytosolic AKT phosphorylation and activation of AKT-mTOR-4EBP1 signaling in esophageal squamous cell carcinoma. Co-immunoprecipitation (SNORD12B-PP1α and 14-3-3ζ-PP1α interactions), subcellular fractionation, western blotting for AKT phosphorylation, RNA interference and overexpression, in vivo tumor models Oncogene Medium 33941854
2024 The peptide PDHK1-241aa (encoded by circPDHK1) interacts with PPP1CA and causes its relocation to the nucleus. This nuclear relocation of PPP1CA inhibits AKT dephosphorylation and activates the AKT-mTOR signaling pathway to promote clear cell renal cell carcinoma progression. Immunoprecipitation-mass spectrometry (identifying PPP1CA as PDHK1-241aa interactor), co-immunoprecipitation, immunofluorescence, subcellular fractionation, AKT phosphorylation western blot, functional assays in ccRCC cells and nude mice Molecular cancer Medium 38360682
2019 MIIP interacts with PP1α via its C-terminal domain but does not affect PP1α protein level. MIIP interaction with PP1α enhances PP1α-mediated AKT dephosphorylation. Silencing PP1α reverses MIIP's inhibitory effect on AKT phosphorylation and cell growth; MIIPC lacking the PP1α-interacting C-terminus loses MIIP's tumor-suppressive function. Co-immunoprecipitation, immunofluorescence co-localization, western blotting for AKT phosphorylation, siRNA knockdown of PP1α, MIIP deletion mutants (MIIP∆C), xenograft assays Cell communication and signaling Medium 31092266
1997 PP1α is phosphorylated by PKC at Ser-325 (in its C-terminal region) in response to B-cell receptor (IgM) stimulation. PP1 activity transiently decreases after B-cell stimulation. PP1alpha undergoes isoform-specific modification (altered isoelectric point pattern on 2D gels) upon BCR stimulation or PMA treatment, while PP1delta does not. The C-terminal peptide of PP1alpha is a better PKC substrate than PP1gamma1/2. 2D electrophoresis + western blot, in vitro phosphorylation of C-terminal peptides by PKC with [gamma-32P]ATP, PP1 activity assays, B-cell stimulation model Journal of biochemistry Medium 9399575
2024 In Zika virus infection, NS2B acts as a scaffold protein mediating interaction between PP1α and eIF2α. This NS2B-PP1α complex promotes eIF2α dephosphorylation by PP1α, inhibiting stress granule formation and promoting viral replication. The NS2B-PP1α complex is stabilized against ubiquitin-induced degradation. The NS2BV35A mutant interacts only with eIF2α and fails to inhibit stress granule formation. Co-immunoprecipitation of NS2B-PP1α-eIF2α complex, eIF2α dephosphorylation assays, stress granule formation assays, NS2BV35A mutagenesis, ubiquitination resistance assay, viral replication assays, brain organoid experiments PLoS pathogens High 38935808
2025 NY-ESO-1 acts as a scaffold protein recruiting the deubiquitinase OTUB1 to PP1α, forming a ternary NY-ESO-1/OTUB1/PP1α complex. OTUB1 deubiquitinase activity (not its E2-suppressing function) prevents PP1α polyubiquitination and promotes its stability. Accumulated PP1α then activates ERK1/2 signaling to promote anoikis resistance and tumor metastasis. Co-immunoprecipitation of ternary complex, ubiquitination assays, OTUB1 deubiquitinase activity assay, PP1α knockdown rescue experiments, ERK1/2 activity measurements, in vivo metastasis assays Cell death & disease Medium 41053024
2025 PP1A dephosphorylates Keap1 at site Ser104 (site 104), disrupting the Keap1-Nrf2 interaction, enhancing transcription of ferroptosis-related markers and immune checkpoint PD-L1, thereby inhibiting Lenvatinib-induced ferroptosis in hepatocellular carcinoma. Transcriptomic analysis, ferroptosis metabolite profiling (ROS, Fe2+, lipid-ROS, GSH), PP1A knockdown, western blotting for Keap1-Nrf2 interaction, PD-L1 expression, single-cell sequencing, co-culture T-cell exhaustion assays, in vivo tumor models Advanced science Medium 40344394
2025 PP1α selectively removes activation-loop phosphorylation from AMPK (dephosphorylating the activation-loop sites) while autophosphorylation sites remain protected, establishing asymmetric phosphatase control of AMPK regulatory states. Phosphatase competition assay (PP1A treatment of phosphorylated AMPK), integrated bottom-up and top-down mass spectrometry for site-specific kinetics, proteoform characterization bioRxivpreprint Medium bio_10.1101_2025.10.10.681638

Source papers

Stage 0 corpus · 71 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2008 PP2B and PP1alpha cooperatively disrupt 7SK snRNP to release P-TEFb for transcription in response to Ca2+ signaling. Genes & development 154 18483222
2019 USP11 promotes growth and metastasis of colorectal cancer via PPP1CA-mediated activation of ERK/MAPK signaling pathway. EBioMedicine 110 31521612
2014 Canonical Wnt signalling activates TAZ through PP1A during osteogenic differentiation. Cell death and differentiation 96 24510127
2020 Processing of the SARS-CoV pp1a/ab nsp7-10 region. The Biochemical journal 93 32083638
2010 PINCH1 regulates Akt1 activation and enhances radioresistance by inhibiting PP1alpha. The Journal of clinical investigation 85 20530873
2006 Proteolytic maturation of replicase polyprotein pp1a by the nsp4 main proteinase is essential for equine arteritis virus replication and includes internal cleavage of nsp7. The Journal of general virology 85 17098961
1999 Processing of the human coronavirus 229E replicase polyproteins by the virus-encoded 3C-like proteinase: identification of proteolytic products and cleavage sites common to pp1a and pp1ab. Journal of virology 84 9847320
1999 Inhibitory phosphorylation of PP1alpha catalytic subunit during the G(1)/S transition. The Journal of biological chemistry 81 10506210
2011 PP1A-mediated dephosphorylation positively regulates YAP2 activity. PloS one 73 21909427
2021 SRPK1/2 and PP1α exert opposite functions by modulating SRSF1-guided MKNK2 alternative splicing in colon adenocarcinoma. Journal of experimental & clinical cancer research : CR 70 33602301
2019 Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis. Nature communications 54 31332168
2008 PPP1CA contributes to the senescence program induced by oncogenic Ras. Carcinogenesis 54 18204081
2013 ERK/PP1a/PLB/SERCA2a and JNK pathways are involved in luteolin-mediated protection of rat hearts and cardiomyocytes following ischemia/reperfusion. PloS one 53 24386130
2023 Arginine methylation of PPP1CA by CARM1 regulates glucose metabolism and affects osteogenic differentiation and osteoclastic differentiation. Clinical and translational medicine 46 37649137
2008 RNA interference in hippocampus demonstrates opposing roles for CREB and PP1alpha in contextual and temporal long-term memory. Genes, brain, and behavior 44 19191880
2024 A novel peptide PDHK1-241aa encoded by circPDHK1 promotes ccRCC progression via interacting with PPP1CA to inhibit AKT dephosphorylation and activate the AKT-mTOR signaling pathway. Molecular cancer 41 38360682
2021 The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma. Cell death and differentiation 41 34799729
2018 Deregulated PP1α phosphatase activity towards MAPK activation is antagonized by a tumor suppressive failsafe mechanism. Nature communications 39 29335436
2017 Lineage A Betacoronavirus NS2 Proteins and the Homologous Torovirus Berne pp1a Carboxy-Terminal Domain Are Phosphodiesterases That Antagonize Activation of RNase L. Journal of virology 36 28003490
2017 Oxidative stress promotes SIRT1 recruitment to the GADD34/PP1α complex to activate its deacetylase function. Cell death and differentiation 35 28984870
2021 LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction. Cancer letters 33 33675985
2008 Drosophila Uri, a PP1alpha binding protein, is essential for viability, maintenance of DNA integrity and normal transcriptional activity. BMC molecular biology 33 18412953
2023 Dietary potassium stimulates Ppp1Ca-Ppp1r1a dephosphorylation of kidney NaCl cotransporter and reduces blood pressure. The Journal of clinical investigation 32 37676724
2018 Involvement of PKA/DARPP-32/PP1α and β- arrestin/Akt/GSK-3β Signaling in Cadmium-Induced DA-D2 Receptor-Mediated Motor Dysfunctions: Protective Role of Quercetin. Scientific reports 29 29410441
2023 Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration. Cell death and differentiation 28 37605006
2006 Identification of PP1alpha as a caspase-9 regulator in IL-2 deprivation-induced apoptosis. Journal of immunology (Baltimore, Md. : 1950) 28 16888006
2005 Protein phosphatase-1 inhibitor-3 is co-localized to the nucleoli and centrosomes with PP1gamma1 and PP1alpha, respectively. Archives of biochemistry and biophysics 28 16256067
2021 Oncogenic SNORD12B activates the AKT-mTOR-4EBP1 signaling in esophageal squamous cell carcinoma via nucleus partitioning of PP-1α. Oncogene 27 33941854
2022 Comprehensive analysis of PPPCs family reveals the clinical significance of PPP1CA and PPP4C in breast cancer. Bioengineered 25 34964699
2014 Sensitive biosensor based on recombinant PP1α for microcystin detection. Biosensors & bioelectronics 23 25459056
2021 PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy. Experimental eye research 22 34280391
2017 Integrin-FAK-CDC42-PP1A signaling gnaws at YAP/TAZ activity to control incisor stem cells. BioEssays : news and reviews in molecular, cellular and developmental biology 22 28891248
2021 Downregulation of lncRNA TSLNC8 promotes melanoma resistance to BRAF inhibitor PLX4720 through binding with PP1α to re-activate MAPK signaling. Journal of cancer research and clinical oncology 21 33389075
2021 Potential 3-chymotrypsin-like cysteine protease cleavage sites in the coronavirus polyproteins pp1a and pp1ab and their possible relevance to COVID-19 vaccine and drug development. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 21 33913206
2014 Standardised extract of Bacopa monniera (CDRI-08) improves contextual fear memory by differentially regulating the activity of histone acetylation and protein phosphatases (PP1α, PP2A) in hippocampus. Cellular and molecular neurobiology 21 24610280
2010 Glioblastoma-specific protein interaction network identifies PP1A and CSK21 as connecting molecules between cell cycle-associated genes. Cancer research 20 20663907
2007 Regulation of intracellular calcium release and PP1alpha in a mechanism for 4-hydroxytamoxifen-induced cytotoxicity. Molecular and cellular biochemistry 20 17646931
2019 The effect of decitabine on the expression and methylation of the PPP1CA, BTG2, and PTEN in association with changes in miR-125b, miR-17, and miR-181b in NALM6 cell line. Journal of cellular biochemistry 18 30912184
2023 CircGPRC5A enhances colorectal cancer progress by stabilizing PPP1CA and inducing YAP dephosphorylation. Journal of experimental & clinical cancer research : CR 17 38057879
2019 Dephosphorylation of the transcriptional cofactor NACA by the PP1A phosphatase enhances cJUN transcriptional activity and osteoblast differentiation. The Journal of biological chemistry 16 30948508
2015 HSV targeting of the host phosphatase PP1α is required for disseminated disease in the neonate and contributes to pathogenesis in the brain. Proceedings of the National Academy of Sciences of the United States of America 16 26621722
2011 Taperin (c9orf75), a mutated gene in nonsyndromic deafness, encodes a vertebrate specific, nuclear localized protein phosphatase one alpha (PP1α) docking protein. Biology open 16 23213405
2019 MIIP inhibits the growth of prostate cancer via interaction with PP1α and negative modulation of AKT signaling. Cell communication and signaling : CCS 13 31092266
1993 Selective high expression of protein phosphatase pp1-alpha messenger-RNA in rat poorly differentiated ascites hepatomas. International journal of oncology 13 21573543
2014 Development and validation of a robust and sensitive assay for the discovery of selective inhibitors for serine/threonine protein phosphatases PP1α (PPP1C) and PP5 (PPP5C). Assay and drug development technologies 12 25383722
2018 The reduced activity of PP-1α under redox stress condition is a consequence of GSH-mediated transient disulfide formation. Scientific reports 11 30531830
2014 14-3-3ζ regulates nuclear trafficking of protein phosphatase 1α (PP1α) in HEK-293 cells. Archives of biochemistry and biophysics 11 24956593
1999 Up-regulation of nuclear PP1alpha and PP1delta in hepatoma cells. International journal of oncology 11 9863017
2013 PP1α, PP1β and Wip-1 regulate H4S47 phosphorylation and deposition of histone H3 variant H3.3. Nucleic acids research 10 23828041
2024 The NS2B-PP1α-eIF2α axis: Inhibiting stress granule formation and Boosting Zika virus replication. PLoS pathogens 9 38935808
2023 KIF18A interacts with PPP1CA to promote the malignant development of glioblastoma. Experimental and therapeutic medicine 9 36911368
2022 PP1A prevents ROS-induced pyroptosis by inhibiting MAPK/caspase-3 in mouse adipose tissue. The FEBS journal 9 35080339
2024 METTL14-mediated upregulation of lncRNA HOTAIR represses PP1α expression by promoting H3K4me1 demethylation in oxycodone-treated mice. CNS neuroscience & therapeutics 8 39046182
2016 An HSV-based library screen identifies PP1α as a negative TRPV1 regulator with analgesic activity in models of pain. Molecular therapy. Methods & clinical development 8 27382601
1995 Comparative mapping of the gene encoding the catalytic subunit of protein phosphatase type 1 alpha (PPP1CA) to human, rat, and mouse chromosomes. Cytogenetics and cell genetics 8 7736790
2023 E2F1-mediated ectopic expression of PP1A promotes breast cancer progression via activation of YAP1. The international journal of biochemistry & cell biology 6 36787863
2025 PP1A Modulates the Efficacy of Lenvatinib Plus ICIs Therapy by Inhibiting Ferroptosis in Hepatocellular Carcinoma. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 4 40344394
2022 Expression and methylation status of BTG2, PPP1CA, and PEG3 genes in colon adenocarcinoma cell lines: promising treatment targets. Gastroenterology and hepatology from bed to bench 4 36762213
1997 Alterations in type-1 serine/threonine protein phosphatase PP1alpha in response to B-cell receptor stimulation. Journal of biochemistry 4 9399575
2025 NY-ESO-1 facilitates anoikis resistance and tumor metastasis by hijacking deubiquitinase OTUB1 to stabilize PP1α. Cell death & disease 3 41053024
2013 PP-1α and PP-1γ display antagonism and differential roles in tumorigenicity of lung cancer cells. Current molecular medicine 3 23176181
2025 PPP1CA promotes hepatocellular carcinoma progression in a YAP1-dependent way. Cellular signalling 1 40513845
2025 Exosomal miR-212-5p promotes tendon repair via targeting FOXO1 to activate PP1A/YAP1 signaling. Communications biology 1 41326793
2024 In-silico analysis of potential anticancer drug for NEK7 and PPP1CA proteins overexpressed in pancreatic ductal adenocarcinoma. Journal of biomolecular structure & dynamics 1 38469816
2024 The epigenetically regulated PP1α expression by KDM1A may contribute to oxycodone conditioned place preference in mice. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 1 38870630
2024 S100A4 targets PPP1CA/IL-17 to inhibit the senescence of sheep endometrial epithelial cells. Frontiers in veterinary science 1 39664902
2023 Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9. Stem cell research 1 36965407
2002 Further in vitro characterization of mouse hepatitis virus papain-like proteinase 1: cleavage sequence requirements within pp1a. Journal of neurovirology 1 11935466
2026 IIIG9 and PP1α form a protein complex in the adherens junctions of polarized ependymal and MDCK cells. Fluids and barriers of the CNS 0 41566508
2026 ELFN2 inhibits YAP-driven lymph node metastasis in gastric cancer by blocking PP1A-mediated dephosphorylation. Experimental cell research 0 41967793
2024 Nodularin-R Synergistically Enhances Abiraterone Against Castrate- Resistant Prostate Cancer via PPP1CA Inhibition. Journal of cellular and molecular medicine 0 39550701

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