Affinage

USP11

Ubiquitin carboxyl-terminal hydrolase 11 · UniProt P51784

Length
963 aa
Mass
109.8 kDa
Annotated
2026-06-10
97 papers in source corpus 61 papers cited in narrative 61 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

USP11 is an X-linked ubiquitin-specific protease (cysteine-protease deubiquitinase) that controls the stability and activity of a broad set of nuclear and cytoplasmic regulatory proteins, thereby governing DNA damage repair, cell-cycle progression, chromatin organization, and growth-factor signaling (PMID:15314155, PMID:29666278). Its substrate selectivity is structurally encoded: a noncatalytic UBL2 domain bearing a peptide-binding interface absent from paralogs USP4/USP15 directs USP11 toward K48-linked polyubiquitin chains and modulates its DNA-repair function (PMID:30373771, PMID:41207628). In the genome-maintenance arena, USP11 acts in BRCA2-dependent homologous recombination and is required for proper RAD51/53BP1 focus formation (PMID:15314155, PMID:20233726); it deubiquitinates the DNA-damage substrates γH2AX, SPRTN, and senataxin to terminate repair signaling, resolve DNA-protein cross-links, and clear R-loops, and supports nucleotide excision repair by stabilizing XPC (PMID:26507658, PMID:33567341, PMID:34526504, PMID:29228550). As a histone deubiquitinase acting on H2AK119ub/H2BK120ub in concert with the NuRD complex, USP11 couples chromatin reorganization to repair termination and also regulates transcription of target loci such as AR and c-Myc (PMID:31504778, PMID:39052835). USP11 is a central rheostat of cell-cycle and tumor-suppressor circuits, stabilizing p21, p53, PTEN, and VGLL4 while also deubiquitinating MDM2 within the MDM2–p53–p21 axis (PMID:29666278, PMID:30733438, PMID:28042509, PMID:38901057). It amplifies TGFβ signaling by stabilizing the receptors ALK5 and TβRII to drive SMAD2/3 activation and EMT (PMID:22773947, PMID:27853171), and it tunes NF-κB signaling through IκBα and IKKα (PMID:19874889, PMID:17897950). USP11 further restrains autophagy by limiting class III PI3K complex activity and stabilizing mTOR, while stabilizing Beclin-1, NRF2, and Sirt3 to modulate autophagic flux and ferroptosis (PMID:34600886, PMID:34839355, PMID:33531626, PMID:37099926). ERK1/2 phosphorylation at Ser905 redistributes USP11 to the cytoplasm to stabilize cytoplasmic p21, linking signaling input to subcellular substrate choice (PMID:35414784). In vivo, Usp11 stabilizes the transcription factor Sox11 to support cortical neurogenesis and neuronal migration, with a disease-associated mutant failing to rescue these functions (PMID:33579706).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2002 High

    Established USP11 as a functional deubiquitinase with a defined nuclear substrate, answering whether the enzyme protects specific proteins from proteasomal turnover.

    Evidence Y2H, Co-IP, in vitro/in vivo deubiquitination and pulse-chase of RanBPM

    PMID:12084015

    Open questions at the time
    • Did not define chain-linkage specificity
    • No structural basis for substrate recognition
  2. 2004 High

    Placed USP11 in the BRCA2 DNA-damage repair pathway, addressing whether the enzyme has a genome-maintenance role beyond isolated substrate stabilization.

    Evidence Immunopurification-MS, reciprocal Co-IP, catalytic mutant, RNAi, mitomycin C survival in BRCA2-dependent manner

    PMID:15314155

    Open questions at the time
    • Direct repair substrate within the pathway not defined
    • Mechanism of recruitment to damage sites unknown
  3. 2010 High

    Demonstrated that USP11 catalytic activity is required for homologous recombination and proper RAD51/53BP1 focus formation, establishing a direct functional requirement in DSB repair.

    Evidence RNAi, HR reporter assay, PARP inhibitor/IR sensitivity, immunofluorescence foci

    PMID:20233726

    Open questions at the time
    • Repair substrate deubiquitinated at foci not yet identified
    • Distinction between HR and NHEJ roles unclear
  4. 2010 High

    Defined USP11 as a deubiquitinase of IκBα and a regulator of NF-κB, with evidence for both catalytic and non-catalytic functions in signaling.

    Evidence Recombinant in vitro DUB assay, knockdown/overexpression, catalytic mutant, NF-κB reporter

    PMID:17897950 PMID:19874889

    Open questions at the time
    • Molecular basis of the non-catalytic NF-κB function unresolved
    • Relationship between IKKα transcriptional and IκBα post-translational roles unclear
  5. 2012 High

    Showed USP11 amplifies TGFβ signaling by deubiquitinating and stabilizing the receptor ALK5, establishing a receptor-level mechanism for promoting EMT.

    Evidence Reciprocal Co-IP with SMAD7, in vitro/in vivo DUB assay, SMAD2/3 phosphorylation and EMT readouts

    PMID:22773947

    Open questions at the time
    • Whether SMAD7 recruits USP11 to the receptor not fully resolved
  6. 2014 High

    Extended USP11 substrate range to tumor-suppressor and apoptotic regulators (PML, p53), and showed transcriptional repression of USP11 by Notch/Hey1 links it to glioma malignancy.

    Evidence RNAi screen, Co-IP, in vivo/in vitro DUB assays, orthotopic mouse model (PML); Co-IP and DUB assay (p53)

    PMID:24487962 PMID:25471832

    Open questions at the time
    • p53 finding rests on limited orthogonal methods
    • Competition between USP11 and the RNF4/KLHL20 ligases not quantified
  7. 2016 High

    Consolidated USP11 as a stabilizer of apoptosis inhibitors and growth regulators (cIAP2, XIAP, VGLL4, TβRII), connecting it to apoptosis resistance and YAP/TGFβ axes in cancer.

    Evidence Co-IP/MS, in vitro/in vivo DUB assays, mutagenesis (XIAP BIR2 Leu207), domain mapping, anoikis/apoptosis and xenograft assays

    PMID:25613375 PMID:27853171 PMID:28040451 PMID:28042509

    Open questions at the time
    • Determinants of substrate selectivity (e.g. cIAP2 over cIAP1) not structurally explained
    • Tumor-suppressor vs oncogenic role appears context-dependent
  8. 2018 High

    Defined USP11 as a master stabilizer of the CDK inhibitor p21 against multiple E3 ligases, establishing direct control over G1/S transition and the G2 DNA-damage checkpoint.

    Evidence In vivo/in vitro DUB assays reversing SCF-SKP2, CRL4-CDT2, APC/C-CDC20 ubiquitylation; cell-cycle and DNA-damage analyses; in vivo tumor model

    PMID:29666278

    Open questions at the time
    • How USP11 outcompetes diverse ligases on a single substrate not defined
  9. 2018 High

    Solved a crystal structure revealing a unique UBL-region peptide-binding site absent in USP4/USP15 that modulates HR repair, providing the first structural basis for paralog-selective function.

    Evidence Phage display, ITC, X-ray crystallography, reporter assay with binding-pocket double mutant

    PMID:30373771

    Open questions at the time
    • Endogenous ligand of the binding pocket not identified
    • Link between pocket occupancy and catalytic output not resolved
  10. 2019 High

    Identified USP11 as a histone deubiquitinase acting with the NuRD complex, and as a node in a PTEN feedforward loop, integrating chromatin reorganization and PI3K/FOXO signaling.

    Evidence In vitro/in vivo histone DUB assays (H2AK119ub/H2BK120ub), Co-IP with NuRD, DNA-repair readouts; Co-IP, DUB assay and Usp11 KO mouse tumor model for PTEN

    PMID:30733438 PMID:31504778

    Open questions at the time
    • Recruitment of USP11 to specific chromatin loci not defined
    • Apparent substrate-specificity discrepancy with earlier γH2AX-only finding [#10] unreconciled
  11. 2021 High

    Expanded the genome-maintenance role to DNA-protein cross-link repair and R-loop homeostasis through deubiquitination of SPRTN and senataxin.

    Evidence In vitro DUB assays on monoubiquitinated SPRTN and K48-ubiquitinated SETX, KO cells, DPC/R-loop/DSB functional readouts, KEAP1 epistasis

    PMID:33567341 PMID:34526504

    Open questions at the time
    • Timing of USP11 action relative to substrate function not resolved
    • Whether these activities require chromatin recruitment cofactors unknown
  12. 2021 High

    Defined a role for USP11 in restraining autophagy and in spindle integrity and cortical neurogenesis, broadening its physiological reach to cell division and neural development.

    Evidence KO cells with autophagic-flux and PI3K activity assays, C. elegans model (autophagy); spindle localization and multipolar-spindle phenotype (RAE1); Usp11 KO mouse with Sox11 DUB assay, neurogenesis, migration and behavior

    PMID:29293652 PMID:33579706 PMID:34600886

    Open questions at the time
    • Direct vs indirect effects on the class III PI3K complex not fully separated
    • Whether a defined Mendelian disease arises from the Sox11-linked mutant not established here
  13. 2022 Medium

    Established phosphorylation-controlled subcellular partitioning of USP11, linking ERK1/2 signaling to cytoplasmic substrate engagement.

    Evidence Phospho-site mapping (Ser905), subcellular fractionation, Co-IP and DUB assay of cytoplasmic p21, proliferation readout

    PMID:35414784

    Open questions at the time
    • Single lab
    • How cytoplasmic vs nuclear p21 pools differ functionally not fully resolved
  14. 2024 High

    Demonstrated dual transcriptional control by USP11 (substrate protein stabilization plus histone-mark erasure at target promoters), unifying its post-translational and chromatin functions for AR and c-Myc.

    Evidence RNA-seq, ChIP-seq with catalytic-inactive mutant, Co-IP, in vivo DUB assays for AR/c-Myc protein and H2A-K119Ub at promoters

    PMID:39052835

    Open questions at the time
    • Generality of the dual mechanism beyond AR/c-Myc loci untested
    • Recruitment specificity to these promoters not defined
  15. 2025 High

    Mapped UBL2 as the determinant of K48-linkage selectivity and identified selective small-molecule inhibitors, providing a biochemical and pharmacological handle on USP11.

    Evidence In vitro Ub-tetramer cleavage, UBL2 deletion/mutation, paralog comparison, AI-based virtual screening and inhibitor assays (Fenoldopam, Olanzapine)

    PMID:41207628

    Open questions at the time
    • Structural mechanism of inhibitor binding not detailed
    • How K48 selectivity reconciles with reported K63-substrate activity unresolved
  16. 2025 Medium

    Revealed regulation of USP11 itself, including IR-induced RRM1-dependent nuclear import for E2F1-driven HR transcription and non-canonical ubiquitination at Cys318 controlling mitotic behavior.

    Evidence Co-IP, nuclear fractionation/NPC binding, ChIP, HR assay (RRM1/E2F1); ubiquitination-site mapping and spindle/slippage assays (SPRYD3-MYCBP2 at C318)

    PMID:39695160 PMID:41052634

    Open questions at the time
    • Both rest on single-lab datasets
    • Functional integration of import control with cytoplasmic ERK-phospho redistribution unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How USP11 achieves substrate and linkage selectivity across its very large substrate set in vivo, and how its many context-dependent oncogenic versus tumor-suppressive roles are determined, remain unresolved.
  • No unifying model reconciling K48 selectivity with reported K63 substrates
  • Determinants directing USP11 to a given substrate in a given cell type unknown
  • Whether a defined human Mendelian disorder is causally linked not established in this corpus

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 12 GO:0016787 hydrolase activity 4 GO:0042393 histone binding 3
Localization
GO:0005634 nucleus 4 GO:0005829 cytosol 3 GO:0005730 nucleolus 1 GO:0005815 microtubule organizing center 1
Pathway
R-HSA-73894 DNA Repair 7 R-HSA-162582 Signal Transduction 5 R-HSA-1640170 Cell Cycle 4 R-HSA-392499 Metabolism of proteins 4 R-HSA-4839726 Chromatin organization 2 R-HSA-9612973 Autophagy 2
Partners
BRCA2E2F1MDM2P53RANBPMRNF4SMAD7XIAP
Complex memberships
NuRD complex

Evidence

Reading pass · 61 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 USP11 localizes primarily to the nucleus in non-dividing cells and physically interacts with RanBPM (a RanGTP-binding protein) as confirmed by yeast two-hybrid and co-immunoprecipitation. USP11 deubiquitinates RanBPM in vitro and in vivo, protecting it from proteasomal degradation. Yeast two-hybrid, co-immunoprecipitation, in vitro/in vivo ubiquitination assay, pulse-chase analysis, immunofluorescence The Biochemical journal High 12084015
2004 USP11 forms a specific complex with BRCA2 (identified by immunopurification-mass spectrometry) and can deubiquitinate BRCA2 in overexpression assays; a catalytically inactive USP11 mutant had no effect on BRCA2 ubiquitination or protein levels. Loss of USP11 function (dominant-negative or RNAi) increased cellular sensitivity to mitomycin C in a BRCA2-dependent manner, placing USP11 in the BRCA2 DNA damage repair pathway. Immunopurification-mass spectrometry, co-immunoprecipitation, catalytic mutant analysis, RNAi knockdown, cell survival assay Molecular and cellular biology High 15314155
2007 USP11 knockdown attenuates IKKα expression at the transcriptional level and enhances NF-κB activation in response to TNFα. USP11 knockdown or IKKα knockdown abrogates p53 induction upon TNFα exposure; ectopic IKKα rescues p53 expression in USP11-silenced cells, establishing USP11 as an upstream regulator of an IKKα-p53 signaling pathway. siRNA knockdown, immunoblotting, reporter assays, rescue experiments The Journal of biological chemistry Medium 17897950
2008 USP11 interacts with HPV-16 E7 (identified by yeast two-hybrid) and stabilizes E7 by removing ubiquitin chains and reducing degradation; a catalytically inactive USP11 mutant abolished deubiquitinating activity and returned E7 to normal degradation rates. USP11-mediated E7 stabilization modulated E7's effects on cell growth. Yeast two-hybrid, co-immunoprecipitation, in vitro deubiquitination assay, catalytic mutant analysis, immunoblotting The Journal of biological chemistry High 18408009
2010 USP11 acts as a deubiquitinase for IκBα: recombinant USP11 catalyzes IκBα deubiquitination in vitro; USP11 overexpression inhibits IκBα ubiquitination and USP11 knockdown enhances TNFα-induced IκBα ubiquitination and NF-κB activation. A catalytically inactive USP11 mutant partially inhibits NF-κB activation, indicating a non-catalytic function as well. Proteomic approach (co-IP identification), in vitro deubiquitination assay, siRNA knockdown, overexpression, catalytic mutant analysis, reporter assay Cellular signalling High 19874889
2010 USP11 participates in homologous recombination (HR) repair of DNA double-strand breaks. RNAi silencing of USP11 causes spontaneous DNA damage response activation, hypersensitivity to PARP inhibition and ionizing radiation, and defective HR repair. USP11 catalytic activity is required for proper recruitment of RAD51 and 53BP1 to repair foci. RNAi screen, siRNA knockdown, PARP inhibitor sensitivity assay, HR repair assay, immunofluorescence foci analysis The Journal of biological chemistry High 20233726
2012 USP11 interacts with SMAD7 and deubiquitylates the type I TGFβ receptor ALK5, thereby stabilizing it and enhancing TGFβ-induced gene transcription. USP11 deubiquitylase activity is required to augment TGFβ signaling. RNAi depletion of USP11 inhibits TGFβ-induced SMAD2/3 phosphorylation, transcriptional responses, and epithelial-to-mesenchymal transition. Co-immunoprecipitation, in vitro/in vivo deubiquitylation assay, RNAi knockdown, reporter assay, SMAD2/3 phosphorylation assay, EMT assay Open biology High 22773947
2013 Mitoxantrone was identified as an inhibitor of USP11 enzymatic activity via high-throughput biochemical screening of 2,000 FDA-approved compounds; mitoxantrone inhibits USP11 activity in vitro with IC50 < 10 nM for PDA cell survival. High-throughput enzymatic screening, in vitro USP11 activity assay, cell viability assay Molecular cancer research : MCR Medium 23696131
2014 USP11 deubiquitinates and stabilizes PML protein, counteracting PML ubiquitin ligases RNF4 and KLHL20-Cul3-Roc1. In glioma, the Notch/Hey1 pathway transcriptionally represses USP11, leading to PML destabilization; Hey1 upregulation correlates with USP11 and PML downregulation and high-grade malignancy. RNAi screening, co-immunoprecipitation, in vivo/in vitro deubiquitination assay, knockdown/overexpression, orthotopic mouse model Nature communications High 24487962
2014 USP11 forms a complex with p53, deubiquitinates p53, and stabilizes it; USP11 knockdown dramatically attenuates p53 induction in response to DNA damage stress. Co-immunoprecipitation, in vivo deubiquitination assay, siRNA knockdown, immunoblotting Journal of Zhejiang University. Science. B Medium 25471832
2015 USP11 deubiquitylates γH2AX (but not canonical ubH2A-K119 or ubH2B-K120 in vitro); USP11 interacts with γH2AX both in vivo and in vitro. USP11 ablation enhances γH2AX ubiquitylation and causes misregulation and prolonged retention of 53BP1 and ubiquitin-conjugated proteins at DSB sites, with cellular hypersensitivity to γ-irradiation. Co-immunoprecipitation, in vitro/in vivo deubiquitylation assay, siRNA knockdown, immunofluorescence foci analysis, clonogenic survival assay The Journal of biological chemistry High 26507658
2015 USP11 interacts with RNF4 (a SUMO-targeted ubiquitin E3 ligase) and deubiquitinates hybrid SUMO-ubiquitin chains to counteract RNF4 activity. USP11 stabilizes SUMO-enriched nuclear bodies, and in response to MMS-induced DNA damage, USP11 counteracts RNF4 to inhibit dissolution of nuclear bodies. Proteomic approach, co-immunoprecipitation, in vitro deubiquitination of SUMO-ubiquitin chains, immunofluorescence The Journal of biological chemistry Medium 25969536
2015 USP11 deubiquitylates and stabilizes cIAP2 specifically (not cIAP1), protecting it from proteasomal degradation. High USP11 expression confers resistance to Smac mimetic-induced cIAP2 degradation and apoptosis; the TNFα/JNK pathway induces USP11 expression to maintain cIAP2 stability. Co-immunoprecipitation, in vitro/in vivo deubiquitylation assay, siRNA knockdown, apoptosis assay, xenograft model Cell death and differentiation High 25613375
2016 USP11 interacts with XIAP (identified by protein complex purification coupled with mass spectrometry) and deubiquitylates it, stabilizing XIAP and thereby inhibiting anoikis and apoptosis to promote mammary tumorigenesis. Leu207 on the BIR2 domain of XIAP is required for interaction with USP11. DUB screen (transformation assay), protein complex purification + MS, co-immunoprecipitation, in vitro deubiquitylation assay, mutagenesis, anoikis/apoptosis assay, tumor colony formation assay EBioMedicine High 28040451
2016 USP11 deubiquitinates and stabilizes VGLL4 protein; the USP domain of USP11 and the N-terminal region of VGLL4 are required for binding. USP11 knockdown promotes cell growth and migration in a YAP-dependent manner, suggesting USP11 acts as a tumor suppressor through the VGLL4/YAP-TEAD regulatory loop. Co-immunoprecipitation, in vivo deubiquitination assay, domain mapping, knockdown/overexpression, cell growth and migration assays American journal of cancer research Medium 28042509
2016 USP11 deubiquitinates and stabilizes TβRII (TGF-β receptor type II), promoting TGFβ-1 signaling including SMAD2/3 phosphorylation and expression of fibronectin and smooth muscle actin; inhibition or knockdown of USP11 increases TβRII ubiquitination and reduces stability. Co-immunoprecipitation, in vitro/in vivo deubiquitination assay, siRNA knockdown, overexpression, immunoblotting Cell death & disease High 27853171
2017 USP11 positively regulates nucleotide excision repair (NER) by deubiquitinating XPC and promoting its retention at UV-induced DNA damage sites. UV irradiation induces USP11 recruitment to chromatin and USP11 interaction with XPC in an XPC-ubiquitination-dependent manner. Co-immunoprecipitation, in vivo deubiquitination assay, chromatin fractionation, UV irradiation assay, NER functional assay Oncotarget Medium 29228550
2018 USP11 deubiquitylates p21 in the nucleus, removing polyubiquitin chains and stabilizing p21 protein. USP11 reverses p21 polyubiquitylation mediated by SCF-SKP2, CRL4-CDT2, and APC/C-CDC20 in a cell-cycle-independent manner. Loss of USP11 causes p21 destabilization, inducing G1/S transition; DNA damage-induced p21 accumulation is abolished in USP11-depleted cells, abrogating the G2 checkpoint. Co-immunoprecipitation, in vivo and in vitro deubiquitylation assay, siRNA knockdown, cell cycle analysis, DNA damage assay, in vivo tumor model Proceedings of the National Academy of Sciences of the United States of America High 29666278
2018 FASN-induced PI3K-S6Kinase signaling phosphorylates USP11, enhancing its interaction with eIF4B. USP11 stabilizes eIF4B by deubiquitination, thereby promoting oncogenic protein translation in DLBCL. Biochemical co-immunoprecipitation, mass spectrometry, pharmacological and genetic inhibition, in vitro/in vivo deubiquitination assay, translation assay Nature communications High 29483509
2018 USP11 deubiquitylates TβRII and stabilizes it in breast cancer cells, enhancing TGFβ downstream signaling and EMT. Modulating USP11 expression altered TβRII stability and breast cancer cell migratory capacity in vitro and metastasis in vivo. Co-immunoprecipitation, in vivo deubiquitination assay, siRNA/overexpression, migration assay, in vivo metastasis model Molecular cancer research : MCR Medium 29724812
2018 USP11 deubiquitinates Snail and stabilizes it, promoting epithelial-to-mesenchymal transition in ovarian cancer cells. Co-immunoprecipitation, in vitro/in vivo deubiquitination assay, functional invasion/migration assay Oncology reports Medium 30569152
2018 A crystal structure of USP11 in complex with a phage-display-identified peptide revealed a previously unknown binding site in USP11's noncatalytic UBL region that is absent in paralogs USP4 and USP15. This binding site modulates USP11's function in homologous recombination-mediated DNA repair as shown by reporter assays with a binding pocket-deficient double mutant. Phage display (NGPD), isothermal titration calorimetry, X-ray crystallography, reporter assay with mutagenesis The Journal of biological chemistry High 30373771
2019 PTEN transcriptionally upregulates USP11 via the PI3K/FOXO pathway; USP11 in turn deubiquitinates and stabilizes PTEN, creating a feedforward regulatory loop. Mice lacking Usp11 display increased susceptibility to PTEN-dependent tumor initiation, growth, and metastasis. Co-immunoprecipitation, in vivo deubiquitination assay, knockout mouse model, tumor assays, FOXO pathway analysis Nature communications High 30733438
2019 USP11 acts as a histone deubiquitinase that catalyzes H2AK119 and H2BK120 deubiquitination. USP11 physically associates with the NuRD chromatin remodeling complex; USP11-mediated histone deubiquitination and NuRD-associated histone deacetylation coordinate to allow timely termination of DNA repair and chromatin reorganization. Co-immunoprecipitation, in vitro/in vivo histone deubiquitination assay, chromatin condensation assay, DNA repair assay Nucleic acids research High 31504778
2019 USP11 stabilizes PPP1CA by deubiquitinating and protecting it from proteasomal degradation; the USP11/PPP1CA complex promotes colorectal cancer progression by activating the ERK/MAPK signaling pathway. LC-MS/MS protein interaction identification, co-immunoprecipitation, in vivo deubiquitination assay, knockdown/overexpression, in vitro and in vivo tumor assays EBioMedicine Medium 31521612
2020 USP11 deubiquitinates ARID1A and stabilizes it; the E3 ligase TRIM32 opposes USP11 by targeting ARID1A for degradation. USP11 depletion promotes ARID1A degradation and SCC development, while TRIM32 depletion inhibits SCC by stabilizing ARID1A. Syndecan-2 (SDC2) is identified as a downstream target of both ARID1A and USP11. Co-immunoprecipitation, in vivo ubiquitination assay, siRNA knockdown, tumor proliferation/metastasis assays Cell reports Medium 31914402
2020 USP11 interacts with NF90 and deubiquitinates it, stabilizing NF90 protein levels in HCC cells; USP11-promoted HCC proliferation and metastasis is dependent on NF90. Mass spectrometry, co-immunoprecipitation, in vivo ubiquitination assay, siRNA knockdown, cell proliferation/metastasis assays American journal of cancer research Medium 32509388
2020 CRL4-DCAF8 E3 ligase and USP11 oppositely regulate the stability of myeloid leukemia factors MLF1 and MLF2: DCAF8 promotes their degradation through ubiquitin-proteasome pathway while USP11 associates with MLF2 (and MLF1) and increases their stability. Co-immunoprecipitation, in vivo ubiquitination assay, immunoblotting Biochemical and biophysical research communications Low 32703400
2021 USP11 deubiquitinates and stabilizes Beclin 1, promoting autophagy activation which in turn enhances ferroptosis after spinal cord ischemia-reperfusion injury. USP11 knockout reduces neuronal ferroptosis and improves functional recovery; autophagy inhibition weakens USP11-mediated ferroptosis. Co-immunoprecipitation, in vivo deubiquitination assay, USP11 KO mouse model, autophagy/ferroptosis functional assays, in vitro knockdown/overexpression Cell death and differentiation Medium 34839355
2021 USP11 deubiquitinates and stabilizes NRF2; USP11 depletion suppresses cell proliferation and induces ferroptotic cell death due to ROS-mediated stress, effects that are largely abrogated by NRF2 overexpression. Co-immunoprecipitation, in vivo deubiquitination assay, siRNA knockdown, overexpression rescue, cell death/proliferation assays Oncogene Medium 33531626
2021 USP11 deubiquitinates SPRTN metalloprotease by cleaving monoubiquitinated SPRTN in cells and in vitro. USP11 depletion impairs SPRTN deubiquitination, promotes SPRTN auto-proteolysis, causes accumulation of unrepaired DNA-protein cross-links, and hypersensitivity to DPC-inducing agents. Co-immunoprecipitation, in vitro deubiquitination assay, siRNA knockdown, DPC repair assay, formaldehyde sensitivity assay The Journal of biological chemistry High 33567341
2021 USP11 deubiquitinates and stabilizes senataxin (SETX), preventing K48-ubiquitination and protein turnover. Loss of USP11 decreases SETX steady-state levels, reduces R-loop dissolution, and causes accumulation of R-loops and DNA double-strand breaks. Compensatory transcriptional downregulation of the E3 ubiquitin ligase KEAP1 partially restores SETX levels in USP11 KO cells. Co-immunoprecipitation, in vivo ubiquitination analysis, USP11 KO cells, R-loop immunofluorescence, DSB assay, KEAP1 overexpression Nature communications High 34526504
2021 USP11 interacts with E2F1 and maintains E2F1 protein stability by removing its ubiquitin. E2F1 regulates USP11 expression at the transcriptional level, forming a positive feedback loop. This E2F1/USP11 axis promotes HCC proliferation, migration, and inhibits autophagy via ERK/mTOR pathway. Co-immunoprecipitation, in vivo ubiquitination assay, ChIP/promoter analysis, siRNA knockdown, functional cell assays Cancer letters Medium 34044068
2021 USP11 deubiquitinates KLF4 by removing K63-dependent polyubiquitination from it, thereby destabilizing KLF4 expression. USP11 depletion inhibits HCC cell growth and chemoresistance by enhancing KLF4 stability. Proteomic approach (IP/MS), co-immunoprecipitation, in vivo ubiquitination assay, siRNA knockdown, cell growth assays Journal of cellular and molecular medicine Medium 34114341
2021 USP11 restricts autophagy: USP11 KO in mammalian cells results in elevated autophagic flux. USP11 coprecipitates with autophagy-specific class III PI3K complex I and limits its interaction with NRBF2, decreasing lipid kinase activity and recruitment of WIPI proteins. USP11 also interacts with and stabilizes mTOR kinase. USP11 homolog depletion in C. elegans triggers hyperactivation of autophagy and protects against amyloid-β aggregation-induced paralysis. Co-immunoprecipitation, KO cell analysis, autophagic flux assay, PI3K activity assay, C. elegans genetic model The Journal of biological chemistry High 34600886
2021 USP11 deubiquitinates RAE1, and USP11 is associated with the mitotic spindle. USP11 knockdown leads to multipolar spindle formation and drastically reduces cell proliferation; USP11 controls RAE1 ubiquitination at the mitotic spindle, modulating its interaction with NuMA. Co-immunoprecipitation, in vivo ubiquitination assay, lentiviral knockdown, immunofluorescence (spindle localization), flow cytometry PloS one Medium 29293652
2021 Mouse Usp11 stabilizes the transcription factor Sox11 via deubiquitination in the developing cerebral cortex. Usp11 deficiency impairs layer 6 neuron production, delays late-born neuronal migration, and disturbs cognition and anxiety behaviors. A disease-associated Usp11 mutant fails to stabilize Sox11 and is unable to support cortical neurogenesis and neuronal migration. Usp11 knockout mouse, in vivo ubiquitination assay, immunoblotting, behavioral testing, cortical layer analysis, mutant rescue experiments Science advances High 33579706
2021 USP11 stabilizes p53 by deubiquitination and p53 transcriptionally represses KLF2, thereby activating the NF-κB pathway and promoting neuroinflammation after intracerebral hemorrhage. USP11 silencing blocks pro-inflammatory cytokine release by downregulating p53, protecting against neurological impairment. siRNA knockdown, co-immunoprecipitation, in vivo deubiquitination assay, ChIP (p53 binding to KLF2 promoter), in vivo rat ICH model Molecular therapy. Methods & clinical development Medium 34141823
2022 USP11 interacts with and deubiquitinates Sirt3, stabilizing it and thereby reducing oxidative stress-induced ferroptosis. USP11 knockout exacerbates intervertebral disc degeneration and is reversed by Sirt3 overexpression. IP/MS, co-immunoprecipitation, in vivo deubiquitination assay, Sirt3 KO mouse, USP11 KO mouse, rescue experiments Redox biology High 37099926
2022 ERK1/2 phosphorylates USP11 at Ser905, which promotes cytoplasmic localization of USP11. In the cytoplasm, USP11 colocalizes and interacts with p21, catalyzing removal of polyubiquitin chains from cytoplasmic p21 and stabilizing it. USP11-mediated stabilization of cytoplasmic p21 induces breast cancer cell proliferation. Phosphorylation mapping, co-immunoprecipitation, subcellular fractionation, in vitro/in vivo deubiquitination assay, knockdown/overexpression, cell proliferation assay International journal of biological sciences Medium 35414784
2022 Non-mitochondrial HK2 directly interacts with CD133 and promotes binding of USP11 to CD133, thereby inhibiting CD133 polyubiquitylation and degradation. USP11-mediated deubiquitination of CD133 enhances cancer stem cell stemness and tumor growth. Co-immunoprecipitation, mass spectrometry, in vitro/in vivo ubiquitination assay, immunofluorescence, flow cytometry Cancer communications Medium 35975322
2023 USP11 interacts with LSH chromatin-remodeling protein and stabilizes it via deubiquitination; erastin treatment disrupts USP11-LSH interaction, increasing LSH ubiquitination and degradation. LSH binds the CYP24A1 promoter to promote its transcription, which inhibits ferroptosis in colorectal cancer cells. Co-immunoprecipitation, in vivo deubiquitination assay, ChIP analysis, siRNA knockdown, ferroptosis assay Cell death & disease Medium 37414755
2023 USP11 interacts with eEF1A1 and deubiquitinates it on Lys439, preventing ubiquitin-mediated degradation. Elevated eEF1A1 binds SP1, driving SP1-dependent HGF gene transcription and PI3K/AKT activation, promoting EMT and metastasis in HCC. Co-immunoprecipitation, in vivo deubiquitination assay with site-specific K439 mutant, ChIP, knockdown/overexpression, functional EMT/metastasis assays Oncogene Medium 37973952
2023 USP11 stabilizes SMYD5 (a lysine methyltransferase) post-transcriptionally in the context of HIV-1 transcription; the Tat cofactor function of USP11 is required for SMYD5 protein level increases in cells expressing Tat. SMYD5 methylates Tat in vitro. siRNA knockdown, immunoblotting, in vitro methylation assay, co-immunoprecipitation Cell reports Low 36897778
2024 USP11 deubiquitinates both AR (androgen receptor) and c-Myc to increase their protein stability, and also deubiquitinates H2A-K119Ub (a repressive histone mark) on promoters of AR and c-Myc genes to increase their transcription. ChIP-seq with catalytic-inactive USP11 mutant confirmed both mechanisms. RNA-seq, ChIP-seq, catalytic-inactive mutant, co-immunoprecipitation, in vivo deubiquitination assay, knockdown/rescue, cell growth assay Proceedings of the National Academy of Sciences of the United States of America High 39052835
2024 USP11 deubiquitinates and stabilizes DNMT3A, opposing degradation by CUL4-DCAF8 E3 ligase. USP11 also enhances DNMT3A SUMOylation by promoting DNMT3A interaction with SUMO E3 ligases, and enhances DNMT3A binding to the polycomb complex and maintains its DNA methyltransferase activity. Co-immunoprecipitation, in vivo ubiquitination/SUMOylation assays, USP11 stable expression rescue, E1 enzyme inhibition, immunoprecipitation with SUMO E3 ligases, MTase activity assay bioRxiv (preprint)preprint Medium 40161590
2024 USP11 deubiquitinates E-cadherin at K738, stabilizing it. USP11 binds E-cadherin through its C-terminal region. This deubiquitinase activity of USP11 maintains luminal differentiation of mammary epithelial cells and suppresses luminal breast cancer; deubiquitinase-inactive USP11 mutant fails to support these functions. USP11 knockout mice, catalytic-inactive mutant, in vivo deubiquitination assay, site-specific K738 mutagenesis, co-immunoprecipitation, domain mapping The Journal of biological chemistry High 39270819
2024 USP11 depletion leads to increased MDM2 polyubiquitination and reduced MDM2 half-life, elevating p53 protein levels and p21 mRNA in a p53-dependent manner. USP11 acts as a deubiquitinase for MDM2, thereby regulating the MDM2-p53-p21 axis and cell cycle progression. USP11 depletion also promotes cellular senescence in a deubiquitinase-activity-dependent manner. siRNA knockdown, polyubiquitination assay, protein half-life analysis, immunoblotting, cell cycle analysis, senescence assay Biochemical and biophysical research communications Medium 38901057
2024 USP11 deubiquitinates TPIT transcription factor and stabilizes it in functioning ACTH pituitary tumors, thereby enhancing POMC transcription and ACTH secretion. USP11 knockdown reduces both POMC transcription and ACTH secretion in vitro and in vivo. Co-immunoprecipitation, in vivo deubiquitination assay, siRNA knockdown, in vivo model, POMC/ACTH secretion assay Acta neuropathologica communications Medium 39910602
2024 USP11 promotes angiogenesis by binding PRDX2 and removing its K63-linked polyubiquitination, facilitating PRDX2 nuclear translocation. Nuclear PRDX2 promotes concurrent nuclear translocation of c-MYC, which enhances transcription of KDR (VEGFR2) and activates VEGFR2 signaling. USP11 KO markedly inhibits angiogenesis in vivo and in vitro. Co-immunoprecipitation, in vivo deubiquitination assay (K63-linkage specificity), USP11 KO mouse model, retinal angiogenesis model, nuclear fractionation, reporter assay Angiogenesis Medium 40199774
2025 The UBL2 domain of USP11 directs its selectivity toward K48-linked polyubiquitin chains; removal of UBL2-IDR reduces ability to cleave K48 chains most significantly. This K48 chain selectivity function of UBL2 is not shared by paralogs USP4 and USP15. FDA-approved drugs Fenoldopam and Olanzapine were identified as selective USP11 inhibitors that act through a unique scaffold. In vitro Ub-tetramer cleavage assay, cell-based K48-ubiquitin analysis, UBL2 domain deletion/mutation, paralog comparison, AI-based virtual screening, in vitro and cell-based inhibitor assays The Journal of biological chemistry High 41207628
2025 RRM1 interacts with USP11 in the cytoplasm; ionizing radiation-induced recruitment of RRM1 to LaminB1 facilitates USP11 binding to the nuclear pore complex and nuclear translocation of USP11. In the nucleus, USP11 binds E2F1 and prevents ubiquitin-mediated E2F1 degradation, enhancing transcription of RAD51AP1 to promote homologous recombination. Co-immunoprecipitation, nuclear fractionation, NPC binding assay, in vivo ubiquitination assay, ChIP, dominant-negative RRM1 mutant, HR repair assay Cell death discovery Medium 39695160
2025 SPRYD3-MYCBP2 E3 ligase complex promotes ubiquitination of USP11 specifically at cysteine 318 (non-canonical ubiquitination), which promotes bipolar spindle formation and mitotic slippage in the presence of microtubule targeting drugs. Co-immunoprecipitation, ubiquitination site mapping (C318), site-directed mutagenesis, mitotic spindle assay, mitotic slippage assay The Journal of biological chemistry Medium 41052634
2025 USP11 deubiquitinates and stabilizes HINT3, and HINT3 interacts with BCL2. USP11 overexpression increases BCL2 levels, and HINT3 knockdown depletes BCL2, suggesting USP11 promotes endothelial apoptosis resistance in pulmonary arterial hypertension through HINT3-BCL2. Co-immunoprecipitation, in vivo ubiquitination/degradation assay, knockdown/overexpression, immunoblotting Journal of respiratory biology and translational medicine Low 40376595
2025 USP11 interacts with and deubiquitinates GSK3β, modulating its Ser9 phosphorylation level and enzymatic activity, thereby impairing synaptic integrity in the prefrontal cortex under chronic stress. USP11 deficiency protects against stress-induced synaptic injury and depression-like behaviors via GSK3β/mTOR signaling. IP-MS, co-immunoprecipitation, in vivo deubiquitination assay, phosphorylation analysis, USP11 KO mouse, behavioral testing Neurobiology of stress Medium 41889422
2025 USP11 interacts with and deubiquitinates PGAM5, stabilizing it in neurotoxic astrocytes after intracerebral hemorrhage. PGAM5 promotes mPTP opening and Drp1-mediated mitochondrial fission, amplifying mtDNA leakage and cGAS-STING pathway activation. Astrocyte-specific Usp11 KO suppresses neurotoxic astrocyte reactivity and improves neurological recovery. Co-immunoprecipitation, in vivo ubiquitination assay, astrocyte-specific KO mouse, mitochondrial function assay, mtDNA quantification, cGAS-STING pathway analysis Advanced science Medium 41201111
2025 Usp11 maintains survival of marginal zone B cells by regulating ubiquitination of Notch ligands DLL1 and JAG2; Usp11-/- mice show higher MZ B cell survival rates after total body irradiation. Co-IP, ubiquitination assay, Usp11-/- mouse model, flow cytometry, single-cell sequencing, histological analysis Cell death & disease Medium 39904982
2025 USP11 deubiquitinates OTUD5, and this mechanism implicates the OTUD5-STING signaling pathway in the inflammatory response of endothelial cells after radiation. USP11 KO mice show decreased radiation-induced pneumonitis with reduced proinflammatory cytokines. Comprehensive proteome and ubiquitinome analysis, co-immunoprecipitation, Western blot, Usp11-/- mouse model, cytokine measurement International journal of radiation oncology, biology, physics Medium 38364946
2025 USP11 stabilizes SFTPC (surfactant protein C) by interacting with, deubiquitinating it, and extending its half-life; USP11 stabilizes mutant SFTPC-I73T significantly more than wild-type. USP11 depletion in hiPSC-derived alveolar organoids mitigates SFTPC-I73T-induced fibrosis. CRISPR/Cas9 screen, immunoprecipitation, TUBEs assay for ubiquitination, hiPSC-derived alveolar organoids, bleomycin mouse model Theranostics Medium 40225577
2025 USP11 stabilizes EGFR and TRAF6 by removing K48-linked ubiquitin chains, preventing their proteasomal degradation; this potentiates both EGFR and Toll-like receptor signaling in colorectal cancer cells. Co-immunoprecipitation, in vivo K48-ubiquitination assay, USP11 KO and overexpression cell lines, xenograft model, mitoxantrone inhibition Cell death & disease Medium 41419456
2025 USP11 deubiquitinates CK2α, stabilizing its protein level and promoting mitochondrial dysfunction. USP11 KO in mouse prefrontal cortex protects against stress-induced synaptic injury and depressive-like behaviors, effects reversed by CK2α stabilization. IP-MS, co-immunoprecipitation, in vivo deubiquitination assay, USP11 KO mouse, CK2α inhibitor (CX4945), primary neuronal cultures CNS neuroscience & therapeutics Medium 42231570

Source papers

Stage 0 corpus · 97 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2021 USP11 regulates autophagy-dependent ferroptosis after spinal cord ischemia-reperfusion injury by deubiquitinating Beclin 1. Cell death and differentiation 193 34839355
2023 The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3. Redox biology 145 37099926
2004 BRCA2 is ubiquitinated in vivo and interacts with USP11, a deubiquitinating enzyme that exhibits prosurvival function in the cellular response to DNA damage. Molecular and cellular biology 139 15314155
2018 Deubiquitylation and stabilization of p21 by USP11 is critical for cell-cycle progression and DNA damage responses. Proceedings of the National Academy of Sciences of the United States of America 138 29666278
2010 Sensitivity to poly(ADP-ribose) polymerase (PARP) inhibition identifies ubiquitin-specific peptidase 11 (USP11) as a regulator of DNA double-strand break repair. The Journal of biological chemistry 115 20233726
2019 USP11 promotes growth and metastasis of colorectal cancer via PPP1CA-mediated activation of ERK/MAPK signaling pathway. EBioMedicine 110 31521612
2013 Mitoxantrone targets human ubiquitin-specific peptidase 11 (USP11) and is a potent inhibitor of pancreatic cancer cell survival. Molecular cancer research : MCR 104 23696131
2012 USP11 augments TGFβ signalling by deubiquitylating ALK5. Open biology 100 22773947
2014 USP11 regulates PML stability to control Notch-induced malignancy in brain tumours. Nature communications 92 24487962
2021 The deubiquitinase USP11 regulates cell proliferation and ferroptotic cell death via stabilization of NRF2 USP11 deubiquitinates and stabilizes NRF2. Oncogene 85 33531626
2010 USP11 negatively regulates TNFalpha-induced NF-kappaB activation by targeting on IkappaBalpha. Cellular signalling 85 19874889
2018 Fatty Acid Synthase induced S6Kinase facilitates USP11-eIF4B complex formation for sustained oncogenic translation in DLBCL. Nature communications 77 29483509
2002 Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM. The Biochemical journal 77 12084015
2019 USP11 acts as a histone deubiquitinase functioning in chromatin reorganization during DNA repair. Nucleic acids research 70 31504778
2016 Regulation of XIAP Turnover Reveals a Role for USP11 in Promotion of Tumorigenesis. EBioMedicine 67 28040451
2019 PTEN self-regulates through USP11 via the PI3K-FOXO pathway to stabilize tumor suppression. Nature communications 64 30733438
2022 A non-metabolic function of hexokinase 2 in small cell lung cancer: promotes cancer cell stemness by increasing USP11-mediated CD133 stability. Cancer communications (London, England) 63 35975322
2015 USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics. Cell death and differentiation 63 25613375
2007 The deubiquitinating enzyme USP11 controls an IkappaB kinase alpha (IKKalpha)-p53 signaling pathway in response to tumor necrosis factor alpha (TNFalpha). The Journal of biological chemistry 60 17897950
2015 USP11 Is a Negative Regulator to γH2AX Ubiquitylation by RNF8/RNF168. The Journal of biological chemistry 57 26507658
2016 De-ubiquitinating enzyme, USP11, promotes transforming growth factor β-1 signaling through stabilization of transforming growth factor β receptor II. Cell death & disease 56 27853171
2015 Evolution of the highly networked deubiquitinating enzymes USP4, USP15, and USP11. BMC evolutionary biology 54 26503449
2008 USP11 stabilizes HPV-16E7 and further modulates the E7 biological activity. The Journal of biological chemistry 52 18408009
2018 USP11 Enhances TGFβ-Induced Epithelial-Mesenchymal Plasticity and Human Breast Cancer Metastasis. Molecular cancer research : MCR 51 29724812
2014 USP11 regulates p53 stability by deubiquitinating p53. Journal of Zhejiang University. Science. B 51 25471832
2020 TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma. Cell reports 47 31914402
2021 The E2F1/USP11 positive feedback loop promotes hepatocellular carcinoma metastasis and inhibits autophagy by activating ERK/mTOR pathway. Cancer letters 43 34044068
2023 USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1. Cell death & disease 36 37414755
2015 Ubiquitin-specific Protease 11 (USP11) Deubiquitinates Hybrid Small Ubiquitin-like Modifier (SUMO)-Ubiquitin Chains to Counteract RING Finger Protein 4 (RNF4). The Journal of biological chemistry 36 25969536
2021 Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization. Science advances 35 33579706
2018 Upregulation of USP11 promotes epithelial‑to‑mesenchymal transition by deubiquitinating Snail in ovarian cancer. Oncology reports 35 30569152
2016 Ubiquitin-specific protease 11 (USP11) functions as a tumor suppressor through deubiquitinating and stabilizing VGLL4 protein. American journal of cancer research 31 28042509
2018 Discovery of peptide ligands targeting a specific ubiquitin-like domain-binding site in the deubiquitinase USP11. The Journal of biological chemistry 30 30373771
2022 Deubiquitinating enzyme USP11 promotes renal tubular cell senescence and fibrosis via inhibiting the ubiquitin degradation of TGF-β receptor II. Acta pharmacologica Sinica 29 36045219
2021 USP11 degrades KLF4 via its deubiquitinase activity in liver diseases. Journal of cellular and molecular medicine 28 34114341
2021 USP11 controls R-loops by regulating senataxin proteostasis. Nature communications 28 34526504
2021 USP11 facilitates colorectal cancer proliferation and metastasis by regulating IGF2BP3 stability. American journal of translational research 26 33594305
2021 Circ_DOCK1 regulates USP11 through miR-132-3p to control colorectal cancer progression. World journal of surgical oncology 26 33685455
2021 A pro-inflammatory mediator USP11 enhances the stability of p53 and inhibits KLF2 in intracerebral hemorrhage. Molecular therapy. Methods & clinical development 26 34141823
2020 Aberrant USP11 expression regulates NF90 to promote proliferation and metastasis in hepatocellular carcinoma. American journal of cancer research 25 32509388
2020 A Functional Genomic Screen Identifies the Deubiquitinase USP11 as a Novel Transcriptional Regulator of ERα in Breast Cancer. Cancer research 25 33004351
2024 USP11 promotes prostate cancer progression by up-regulating AR and c-Myc activity. Proceedings of the National Academy of Sciences of the United States of America 23 39052835
2023 USP11 potentiates HGF/AKT signaling and drives metastasis in hepatocellular carcinoma. Oncogene 23 37973952
2021 USP11 mediates repair of DNA-protein cross-links by deubiquitinating SPRTN metalloprotease. The Journal of biological chemistry 22 33567341
2017 Regulation of XPC deubiquitination by USP11 in repair of UV-induced DNA damage. Oncotarget 22 29228550
2024 DLGAP5 triggers proliferation and metastasis of bladder cancer by stabilizing E2F1 via USP11. Oncogene 20 38182895
2021 USP11 induce resistance to 5-Fluorouracil in Colorectal Cancer through activating autophagy by stabilizing VCP. Journal of Cancer 20 33758608
2018 USP11 deubiquitinates RAE1 and plays a key role in bipolar spindle formation. PloS one 20 29293652
2024 USP11 promotes glycolysis by regulating HIF-1α stability in hepatocellular carcinoma. Journal of cellular and molecular medicine 18 38229475
2015 USP11, Deubiquitinating Enzyme, Associated with Neuronal Apoptosis Following Intracerebral Hemorrhage. Journal of molecular neuroscience : MN 18 26334325
2024 USP11 promotes renal tubular cell pyroptosis and fibrosis in UUO mice via inhibiting KLF4 ubiquitin degradation. Acta pharmacologica Sinica 17 39147900
2022 The Dual Role of USP11 in Cancer. Journal of oncology 17 35359344
2024 USP11 Exacerbates Radiation-Induced Pneumonitis by Activating Endothelial Cell Inflammatory Response via OTUD5-STING Signaling. International journal of radiation oncology, biology, physics 16 38364946
2020 The regulation of NONO by USP11 via deubiquitination is linked to the proliferation of melanoma cells. Journal of cellular and molecular medicine 16 33369124
2023 The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11. Cell reports 15 36897778
2022 The RSL3 Induction of KLK Lung Adenocarcinoma Cell Ferroptosis by Inhibition of USP11 Activity and the NRF2-GSH Axis. Cancers 14 36358651
2024 The USP11/Nrf2 positive feedback loop promotes colorectal cancer progression by inhibiting mitochondrial apoptosis. Cell death & disease 13 39617751
2020 CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs). Biochemical and biophysical research communications 13 32703400
2024 USP11 regulates proliferation and apoptosis of human spermatogonial stem cells via HOXC5-mediated canonical WNT/β-catenin signaling pathway. Cellular and molecular life sciences : CMLS 12 38722330
2024 USP11 promotes lipogenesis and tumorigenesis by regulating SREBF1 stability in hepatocellular carcinoma. Cell communication and signaling : CCS 12 39558331
2023 USP11 exacerbates neuronal apoptosis after traumatic brain injury via PKM2-mediated PI3K/AKT signaling pathway. Brain research 12 36898475
2021 Inhibition of USP11 sensitizes gastric cancer to chemotherapy via suppressing RhoA and Ras-mediated signaling pathways. Clinics and research in hepatology and gastroenterology 12 34332125
2021 The deubiquitinase USP11 is a versatile and conserved regulator of autophagy. The Journal of biological chemistry 10 34600886
2021 Targeting USP11 may alleviate radiation-induced pulmonary fibrosis by regulating endothelium tight junction. International journal of radiation biology 10 34705600
2024 The deubiquitinating enzyme USP11 regulates breast cancer progression by stabilizing PGAM5. Breast cancer research : BCR 8 39300548
2024 Targeting USP11 regulation by a novel lithium-organic coordination compound improves neuropathologies and cognitive functions in Alzheimer transgenic mice. EMBO molecular medicine 8 39394468
2022 ERK-mediated Cytoplasmic Retention of USP11 Contributes to Breast Cancer Cell Proliferation by Stabilizing Cytoplasmic p21. International journal of biological sciences 8 35414784
2015 USP11: A key regulator of cIAP2 stability and sensitivity to SMAC mimetics. Molecular & cellular oncology 7 27314066
2025 Endothelial USP11 drives VEGFR2 signaling and angiogenesis via PRDX2/c-MYC axis. Angiogenesis 6 40199774
2024 RRM1 promotes homologous recombination and radio/chemo-sensitivity via enhancing USP11 and E2F1-mediated RAD51AP1 transcription. Cell death discovery 6 39695160
2023 USP11 plays a critical role in the onset and progression of acute graft-versus-host disease：Novel target for precision therapeutics. Pharmacological research 6 36822452
1998 UHX1 and PCTK1: precise characterisation and localisation within a gene-rich region in Xp11.23 and evaluation as candidate genes for retinal diseases mapped to Xp21.1-p11.2. European journal of human genetics : EJHG 6 9801870
2024 USP11 deubiquitinates E-cadherin and maintains the luminal fate of mammary tumor cells to suppress breast cancer. The Journal of biological chemistry 4 39270819
2025 Targeting USP11 counteracts SFTPC -associated interstitial lung disease in hiPSCs-derived alveolar organoids and in vivo models. Theranostics 3 40225577
2024 USP11 modulates mitotic progression and senescence by regulating the p53-p21 axis through MDM2 deubiquitination. Biochemical and biophysical research communications 3 38901057
2025 Usp11 maintained the survival of marginal zone B cells under ionizing radiation by deubiquitinating DLL1 and JAG2. Cell death & disease 2 39904982
2025 X-linked ubiquitin-specific peptidase 11 (USP11) increases susceptibility to Cushing's disease in women. Acta neuropathologica communications 2 39910602
2025 'Nelfinavir sensitizes a clinically relevant chemo-radioresistant cervical cancer in-vitro model by targeting the AKT-USP15/USP11-HPV16 E6/E7 axis. Biochemistry and biophysics reports 2 40242402
2025 USP11-PGAM5 Axis Promotes Neurotoxic Astrocyte Reactivity by Aggravating the mtDNA-cGAS-STING Pathway After Intracerebral Hemorrhage. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2 41201111
2026 Golgi fragmentation driven by the USP11-ITCH axis triggers autolysosomal failure in neurodegeneration. Autophagy 1 41655130
2025 USP11 promotes autophagy to attenuate LPS-induced oxidative stress in lung epithelial cells by stabilizing FOXO1 levels. Biochemical and biophysical research communications 1 39908907
2025 Stability and DNA Methyltransferase Activity of DNMT3A are Maintained by Ubiquitin-Specific Peptidase 11 (USP11) and Sumoylation Countering Degradation. bioRxiv : the preprint server for biology 1 40161590
2025 Identification of chemical scaffolds for targeting ubiquitin-specific protease 11 (USP11) through high-throughput virtual screening. Journal of enzyme inhibition and medicinal chemistry 1 40588719
2025 Inhibition of USP11 attenuates sepsis-associated acute kidney injury by downregulating TGFBR2/Smad3 signaling. Frontiers in molecular biosciences 1 40656894
2025 USP11 stabilizes RALY to promote FXYD5-mediated aerobic glycolysis and aggravate pancreatic cancer progression. Experimental cell research 1 40972883
2025 Sevoflurane suppresses LPS-induced microglia injury by regulating USP11-mediated UHRF1 deubiquitination. Regenerative therapy 1 41159065
2025 Forsythiaside A ameliorates acetaminophen-induced liver injury by suppressing ferroptosis through activating USP11/SIRT3-mediated mitophagy. Journal of ethnopharmacology 1 41338315
2025 USP11 promotes colorectal cancer progression by stabilizing EGFR and TRAF6: a potential therapeutic target in EGFR- and TLR-driven tumorigenesis. Cell death & disease 1 41419456
2026 USP11 drives stress-induced synaptic structural deficits and depression-like behaviors through GSK3β/mTOR signaling. Neurobiology of stress 0 41889422
2026 X-Linked USP11 Drives Depression-Like Behaviors by Stabilizing CK2α and Disrupting Mitochondrial Function. CNS neuroscience & therapeutics 0 42231570
2025 USP11 Promotes Endothelial Apoptosis-Resistance in Pulmonary Arterial Hypertension by Deubiquitinating HINT3. Journal of respiratory biology and translational medicine 0 40376595
2025 Mechanism of USP11 regulated SIRT3/ROS in drug resistance of acute myeloid leukemia. Clinical and experimental medicine 0 40736712
2025 USP11 is involved in the sensitivity of liver cancer cells to ferroptosis and taxanes through the regulation of NRF2 ubiquitin-mediated degradation. Translational oncology 0 41033073
2025 The E3 ubiquitin ligase SPRYD3-MYCBP2(PAM) regulates mitotic cell fate and ubiquitination of USP11 to control spindle assembly. The Journal of biological chemistry 0 41052634
2025 Non-catalytic UBL2 domain directs deubiquitinase USP11 toward K48-linked polyubiquitin chains. The Journal of biological chemistry 0 41207628
2025 The Rac1-USP11 feedback amplification loop: a radiation-activated engine driving radioresistance in hepatocellular carcinoma. British journal of cancer 0 41298816
2025 Discovery, development, and characterization of potent and selective USP11 inhibitors. Pharmacological research 0 41461277

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