Affinage

USP11

Ubiquitin carboxyl-terminal hydrolase 11 · UniProt P51784

Length
963 aa
Mass
109.8 kDa
Annotated
2026-04-28
96 papers in source corpus 54 papers cited in narrative 54 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

USP11 is a ubiquitin-specific protease that functions as a broad-specificity deubiquitinase integrating DNA damage repair, cell-cycle control, TGF-β signaling, NF-κB signaling, autophagy, ferroptosis, and chromatin regulation. Its non-catalytic UBL2 domain confers selectivity toward K48-linked polyubiquitin chains, and USP11 stabilizes diverse substrates—including BRCA2, γH2AX, XPC, SPRTN, and senataxin in DNA repair; p21, p53, PTEN, and PML in tumor suppression; ALK5/TβRI and TβRII in TGF-β signaling; IκBα in NF-κB signaling; and Beclin 1 and mTOR in autophagy regulation—by catalyzing their deubiquitination and preventing proteasomal degradation (PMID:15314155, PMID:26507658, PMID:29666278, PMID:30733438, PMID:22773947, PMID:19874889, PMID:34600886, PMID:41207628). USP11 also acts as a histone deubiquitinase for H2AK119ub and H2BK120ub in association with the NuRD complex, coordinating chromatin remodeling with DNA repair termination and transcriptional regulation of AR and c-Myc target genes (PMID:31504778, PMID:39052835). USP11 is itself regulated by ERK1/2 phosphorylation at Ser905 (promoting cytoplasmic relocalization), non-canonical ubiquitination at Cys318 by the MYCBP2-SPRYD3 E3 ligase (modulating mitotic function), and transcriptional control through Notch/Hey1 repression and PTEN-PI3K-FOXO or E2F1 feedforward loops (PMID:35414784, PMID:41052634, PMID:24487962, PMID:30733438, PMID:34044068).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2002 High

    Establishing USP11 as a nuclear deubiquitinase: the initial identification of USP11 as a DUB that interacts with and stabilizes RanBPM via deubiquitination demonstrated that USP11 possesses genuine catalytic deubiquitinating activity and localizes primarily to the nucleus.

    Evidence Yeast two-hybrid, co-IP, in vitro DUB assay with recombinant USP11, immunofluorescence in mammalian cells

    PMID:12084015

    Open questions at the time
    • Physiological role of RanBPM stabilization unclear
    • No structural information on USP11 catalytic domain
    • Substrate scope unknown beyond RanBPM
  2. 2004 High

    Linking USP11 to DNA damage repair: the discovery that USP11 forms complexes with BRCA2, deubiquitinates it in a catalytic-activity-dependent manner, and that USP11 loss sensitizes cells to mitomycin C established USP11 as a participant in the BRCA2-dependent homologous recombination pathway.

    Evidence Immunopurification-MS, co-IP, catalytic-dead mutant, RNAi, MMC survival assay

    PMID:15314155

    Open questions at the time
    • Direct in vitro reconstitution of BRCA2 deubiquitination not shown
    • Mechanism connecting USP11-BRCA2 to RAD51 loading not defined
    • Whether USP11 acts on other HR factors unknown at this point
  3. 2010 High

    Expanding USP11 into NF-κB signaling and confirming HR requirement: two independent studies showed USP11 deubiquitinates IκBα to restrain NF-κB (with both catalytic and non-catalytic functions demonstrated) and that an unbiased RNAi screen confirmed USP11 is required for HR repair with effects on RAD51/53BP1 foci and PARP inhibitor sensitivity.

    Evidence In vitro DUB assay with recombinant USP11 on IκBα; RNAi synthetic-lethality screen with PARP inhibitor; HR reporter assay; immunofluorescence

    PMID:19874889 PMID:20233726

    Open questions at the time
    • IκBα ubiquitin chain-type specificity not determined
    • Precise HR step regulated by USP11 not identified
    • Relationship between NF-κB and DNA repair functions of USP11 unclear
  4. 2012 High

    Connecting USP11 to TGF-β signaling: the demonstration that USP11 deubiquitinates ALK5/TβRI and later TβRII, promoting SMAD2/3 phosphorylation, transcriptional responses, and EMT, established USP11 as a positive regulator of TGF-β receptor stability and downstream signaling.

    Evidence Co-IP, ubiquitination assays, RNAi, phospho-SMAD2/3 readouts, EMT assays; independently confirmed for TβRII

    PMID:22773947 PMID:27853171 PMID:29724812

    Open questions at the time
    • Whether USP11 acts on both receptors simultaneously or in different contexts not resolved
    • Structural basis of USP11-receptor interaction unknown
    • Upstream signals controlling USP11 activity in TGF-β context not defined
  5. 2014 High

    Revealing transcriptional regulation of USP11 and tumor-suppressor substrate scope: the finding that Notch/Hey1 represses USP11 transcription (destabilizing PML) and that USP11 deubiquitinates p53 expanded the substrate repertoire to major tumor suppressors and showed USP11 itself is transcriptionally regulated in cancer contexts.

    Evidence RNAi screen for PML regulators, co-IP, ubiquitination assays, orthotopic glioma model; separate co-IP/ubiquitination study for p53

    PMID:24487962 PMID:25471832

    Open questions at the time
    • Direct transcriptional mechanism (Hey1 binding site on USP11 promoter) not mapped
    • p53 deubiquitination lacks in vitro reconstitution
    • Relative contribution of PML vs. p53 stabilization to tumor suppression unknown
  6. 2015 High

    Defining USP11 as a γH2AX deubiquitinase and SUMO-ubiquitin chain editor: USP11 was shown to specifically deubiquitinate RNF8/RNF168-dependent γH2AX ubiquitination (but not H2A-K119ub or H2B-K120ub in vitro at that time), regulating 53BP1 retention at DSBs, and separately to cleave hybrid SUMO-ubiquitin chains counteracting RNF4, stabilizing SUMO-enriched nuclear bodies during DNA damage.

    Evidence In vitro and in vivo DUB assays on γH2AX and SUMO-Ub chains, siRNA, irradiation sensitivity, MMS treatment

    PMID:25969536 PMID:26507658

    Open questions at the time
    • Ubiquitin chain type on γH2AX not specified
    • Whether SUMO-Ub cleavage and γH2AX deubiquitination are coordinated is unknown
    • Temporal regulation of USP11 at DSBs not determined
  7. 2018 High

    Establishing USP11 as a cell-cycle regulator through p21 stabilization and revealing structural features: USP11 was shown to stabilize p21 by counteracting three distinct E3 ligases (SCF-SKP2, CRL4-CDT2, APC/C-CDC20), controlling G1/S transition and the G2 checkpoint; concurrently, crystal structure of the USP11 UBL domain revealed a paralog-unique peptide-binding site required for HR function.

    Evidence Co-IP, ubiquitination with multiple E3s, cell cycle analysis, in vivo tumor assay; X-ray crystallography, ITC, mutagenesis, HR reporter

    PMID:29666278 PMID:30373771

    Open questions at the time
    • How USP11 is recruited to p21 in specific cell-cycle phases unclear
    • Full-length USP11 structure not solved
    • Whether the UBL-binding site recruits specific cofactors during HR not known
  8. 2019 High

    Discovering USP11 as a histone deubiquitinase and a PTEN stabilizer in a feedforward loop: USP11 was found to catalyze H2AK119 and H2BK120 deubiquitination in association with the NuRD complex for DNA repair termination, and separately, PTEN transcriptionally induces USP11 via PI3K/FOXO while USP11 deubiquitinates PTEN, creating a tumor-suppressive feedforward loop validated in Usp11 KO mice.

    Evidence In vitro histone DUB assay with NuRD co-IP, chromatin fractionation; transcriptional reporter, ubiquitination assay, Usp11 KO mice with tumor phenotype

    PMID:30733438 PMID:31504778

    Open questions at the time
    • Which NuRD subunit directly recruits USP11 not identified
    • Whether histone deubiquitination and PTEN stabilization are linked in the same cells unclear
    • PTEN-FOXO-USP11 loop not validated in human tumors in vivo
  9. 2021 High

    Expanding USP11 into autophagy, ferroptosis, R-loop resolution, DPC repair, and cortical neurogenesis: a cluster of discoveries showed USP11 stabilizes Beclin 1 to promote autophagy-dependent ferroptosis, stabilizes NRF2 to oppose ferroptosis, deubiquitinates SPRTN for DNA-protein crosslink repair, stabilizes senataxin/SETX to resolve R-loops, interacts with PI3KC3-C1/mTOR to restrain autophagy, and stabilizes Sox11 for proper cortical layer 6 neurogenesis in KO mice.

    Evidence Multiple KO mouse models, in vitro DUB assays, co-IP, autophagy flux, ferroptosis markers, DPC repair assays, R-loop assays, behavioral assays

    PMID:33531626 PMID:33567341 PMID:33579706 PMID:34526504 PMID:34600886 PMID:34839355

    Open questions at the time
    • Context-dependent pro- vs. anti-ferroptotic roles (Beclin 1 vs. NRF2) not reconciled
    • Whether PI3KC3-C1 interaction is direct or indirect not fully resolved
    • Sox11 stabilization relevance to human neurodevelopmental disease not established
  10. 2024 High

    Demonstrating dual substrate-and-chromatin roles and revealing USP11 regulation by phosphorylation and non-canonical ubiquitination: USP11 deubiquitinates both AR/c-Myc proteins and H2A-K119Ub at their gene promoters (ChIP-seq validated); ERK1/2 phosphorylation at Ser905 drives USP11 cytoplasmic localization to stabilize cytoplasmic p21; and the MYCBP2-SPRYD3 E3 ligase ubiquitinates USP11 at Cys318 to regulate bipolar spindle formation.

    Evidence ChIP-seq, RNA-seq, catalytic-dead replacement, phosphosite mapping, subcellular fractionation, spindle assays, mitotic slippage assays

    PMID:35414784 PMID:39052835 PMID:41052634

    Open questions at the time
    • How ERK-mediated relocalization and Cys318 ubiquitination are coordinated during cell cycle unknown
    • Genome-wide H2A-K119Ub targets of USP11 beyond AR/c-Myc not mapped
    • Functional consequences of Cys318 modification on catalytic activity not tested
  11. 2025 High

    Defining chain-type selectivity and expanding therapeutic relevance: the UBL2 domain was shown to confer K48-linked polyubiquitin chain preference distinguishing USP11 from paralogs USP4/USP15; USP11 was validated as a druggable target in pulmonary fibrosis (SFTPC-I73T stabilization) and in radioresistance (Rac1-GTP feedback loop).

    Evidence In vitro Ub-tetramer cleavage assays with domain deletions, CRISPR DUB screen, hiPSC-derived organoid model, bleomycin mouse model, site-specific Rac1 ubiquitination mapping, xenograft

    PMID:40225577 PMID:41207628 PMID:41298816

    Open questions at the time
    • Whether K48 selectivity applies to all substrates or is context-dependent not established
    • No selective, potent USP11 inhibitor with characterized specificity profile exists
    • Whether K63 deubiquitination (e.g. PRDX2) uses a UBL2-independent mechanism not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include how USP11 achieves substrate selectivity among its dozens of reported targets, whether its seemingly opposing roles in ferroptosis (pro- via Beclin 1 vs. anti- via NRF2/Sirt3) reflect tissue- or context-specific activity, and how its multiple regulatory inputs (ERK phosphorylation, MYCBP2 ubiquitination, transcriptional control) are integrated to direct USP11 toward specific substrates in specific cellular compartments.
  • No full-length USP11 structure available
  • No selective pharmacological inhibitor with validated specificity
  • Substrate prioritization mechanism entirely unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 15 GO:0016787 hydrolase activity 6 GO:0042393 histone binding 3
Localization
GO:0005634 nucleus 5 GO:0005694 chromosome 2 GO:0005829 cytosol 1
Pathway
R-HSA-73894 DNA Repair 9 R-HSA-162582 Signal Transduction 7 R-HSA-1640170 Cell Cycle 4 R-HSA-5357801 Programmed Cell Death 4 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-4839726 Chromatin organization 2 R-HSA-9612973 Autophagy 2
Partners
BECN1BRCA2CDKN1APTENSETXSMAD7SPRTNTGFBR2
Complex memberships
NuRD complexPI3KC3-C1 complex

Evidence

Reading pass · 54 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 USP11 was identified as a RanBPM-binding protein via yeast two-hybrid; USP11 interacts with RanBPM in vivo (co-IP), deubiquitinates RanBPM in vitro (recombinant USP11 inhibited ubiquitin conjugation to RanBPM in a dose-dependent manner), and stabilizes RanBPM against proteasomal degradation. USP11 localizes primarily to the nucleus in non-dividing cells. Yeast two-hybrid, co-immunoprecipitation, in vitro deubiquitination assay, pulse-chase analysis with proteasome inhibitors, immunofluorescence The Biochemical journal High 12084015
2004 USP11 forms specific complexes with BRCA2 (immunopurification-MS and Co-IP) and can deubiquitinate BRCA2 in transient overexpression assays; a catalytically inactive USP11 mutant had no effect on BRCA2 ubiquitination or protein levels. USP11 participates in DNA damage repair functions within the BRCA2 pathway, and antagonism of USP11 (dominant-negative mutant or RNAi) increased cellular sensitivity to mitomycin C in a BRCA2-dependent manner. Immunopurification-mass spectrometry, Co-IP, transient overexpression deubiquitination assay, catalytic-dead mutant, RNAi, cell survival assay Molecular and cellular biology High 15314155
2007 USP11 specifically regulates IKKα expression at the transcriptional level. Knockdown of USP11 dramatically enhances NF-κB activity in response to TNFα, and depletion of USP11 or IKKα abrogates p53 induction upon TNFα exposure. Ectopic IKKα expression in USP11-silenced cells restores p53 expression, placing USP11 upstream of an IKKα-p53 signaling pathway. RNAi knockdown, ectopic expression rescue, reporter assays, western blotting The Journal of biological chemistry Medium 17897950
2008 USP11 interacts with HPV-16 E7 (yeast two-hybrid and co-IP), deubiquitinates E7, and stabilizes E7 protein by reducing ubiquitination and attenuating degradation. A catalytically inactive USP11 mutant abolished deubiquitinating ability and returned E7 to normal degradation rates. USP11 also modulates E7 biological function and its contribution to cell transformation. Yeast two-hybrid, Co-IP, ubiquitination assay, catalytic-dead mutant, steady-state protein level analysis The Journal of biological chemistry High 18408009
2010 USP11 was identified as an IκBα-associated deubiquitinase (proteomic approach). Recombinant USP11 catalyzes deubiquitination of IκBα in vitro; USP11 overexpression inhibits IκBα ubiquitination; USP11 knockdown enhances TNFα-induced IκBα ubiquitination and NF-κB activation. A catalytically inactive USP11 mutant partially inhibits TNFα- and IKKβ-induced NF-κB activation, indicating a non-catalytic function as well. Proteomic/Co-IP identification, in vitro deubiquitination assay with recombinant protein, RNAi knockdown, catalytic-dead mutant, NF-κB reporter assay Cellular signalling High 19874889
2010 RNAi screen identified USP11 as required for homologous recombination (HR) repair of DNA double-strand breaks. Silencing USP11 causes spontaneous DDR activation, hypersensitivity to PARP inhibition and ionizing radiation, defective HR, and misregulation of RAD51 and 53BP1 recruitment to repair foci in a manner dependent on USP11 catalytic activity. RNAi screen (synthetic lethality with PARP inhibitor AZD2281), siRNA knockdown, HR repair assay, immunofluorescence foci analysis The Journal of biological chemistry High 20233726
2012 USP11 interacts with SMAD7 and with the type I TGFβ receptor ALK5. USP11 deubiquitylates ALK5, resulting in enhanced TGFβ-induced gene transcription. RNAi depletion of USP11 inhibits TGFβ-induced SMAD2/3 phosphorylation, TGFβ-mediated transcriptional responses, and TGFβ-induced epithelial-to-mesenchymal transition. The deubiquitylase activity of USP11 is required for these effects. Co-IP, ubiquitination assay, RNAi knockdown, phospho-SMAD2/3 western blot, transcription reporter assay, EMT assay Open biology High 22773947
2013 High-throughput biochemical screen of 2,000 FDA-approved compounds identified mitoxantrone as an inhibitor of USP11 enzymatic activity. Mitoxantrone impacts pancreatic cancer cell survival with IC50 <10 nM and shows 40- to 20,000-fold greater potency than gemcitabine across PDA cell lines; USP11 silencing also enhanced gemcitabine sensitivity. High-throughput enzymatic inhibition screen, in vitro drug sensitivity assay, RNAi knockdown Molecular cancer research : MCR Medium 23696131
2014 USP11 deubiquitinates and stabilizes PML, counteracting PML ubiquitin ligases RNF4 and KLHL20-Cul3-Roc1. USP11 was identified by RNAi screening; its expression is transcriptionally repressed by Notch/Hey1, leading to PML destabilization. Notch/Hey1-induced downregulation of USP11/PML confers malignant characteristics including proliferation, invasiveness, and therapeutic resistance in glioma. RNAi screen, Co-IP, ubiquitination assay, loss-of-function with cellular phenotype readouts (proliferation, invasion, tumor growth in orthotopic mouse model) Nature communications High 24487962
2014 USP11 forms specific complexes with p53 (co-IP) and stabilizes p53 by deubiquitinating it. Downregulation of USP11 dramatically attenuates p53 induction in response to DNA damage stress. Co-immunoprecipitation, ubiquitination assay, RNAi knockdown Journal of Zhejiang University. Science. B Medium 25471832
2015 USP11 specifically and selectively deubiquitylates cIAP2 to stabilize it, leading to resistance to Smac mimetics. USP11 overexpression is found in colorectal cancer and melanoma; the TNFα/JNK pathway induces USP11 expression, which maintains cIAP2 stability as an alternative TNFα-dependent cell survival pathway. Co-IP, ubiquitination assay, RNAi knockdown, apoptosis assay, xenograft model Cell death and differentiation High 25613375
2015 USP11 is a negative regulator of γH2AX ubiquitylation by RNF8/RNF168. USP11 deubiquitylates γH2AX both in vivo and in vitro but not canonical H2A-K119ub or H2B-K120ub in vitro. USP11 ablation enhances γH2AX ubiquitylation and causes prolonged retention of 53BP1 and ubiquitin-conjugated proteins at DSB sites, and USP11-depleted cells are hypersensitive to γ-irradiation. In vivo and in vitro deubiquitylation assays, Co-IP, siRNA knockdown, immunofluorescence foci, irradiation sensitivity assay The Journal of biological chemistry High 26507658
2015 USP11 was identified as a functional interactor of RNF4 (proteomic approach) and can deubiquitinate hybrid SUMO-ubiquitin chains to counteract RNF4. USP11 stabilizes SUMO-enriched nuclear bodies and counteracts RNF4-mediated dissolution of nuclear bodies in response to DNA damage (methyl methanesulfonate). Proteomic identification, in vitro deubiquitination of hybrid SUMO-ubiquitin chains, cell-based nuclear body analysis, DNA damage response assay The Journal of biological chemistry Medium 25969536
2016 USP11 was identified as a mammary tumor-promoting DUB by an unbiased transformation screen. XIAP was identified as a USP11 substrate by protein complex purification/MS; USP11 deubiquitylates XIAP to stabilize it, inhibiting cell anoikis and apoptosis and promoting tumorigenesis. Molecular modeling and mutagenesis revealed Leu207 on the BIR2 domain of XIAP facilitates its interaction with USP11. DUB transformation screen, protein complex purification/MS, Co-IP, ubiquitination assay, molecular modeling + mutagenesis, cell transformation assay, apoptosis assay, IHC EBioMedicine High 28040451
2016 USP11 deubiquitinates and stabilizes VGLL4 protein; the USP domain of USP11 and the N-terminal region of VGLL4 are required for mutual binding. USP11 knockdown promotes cell growth, migration, and invasion in a YAP-dependent manner. Co-IP, in vitro/in vivo ubiquitination assay, RNAi knockdown, domain-mapping experiments, cell functional assays American journal of cancer research Medium 28042509
2016 USP11 promotes TGFβ-1 signaling through deubiquitination and stabilization of TβRII (TGFβ receptor type II). Inhibition or downregulation of USP11 increases TβRII ubiquitination and reduces its stability, attenuating SMAD2/3 phosphorylation and downstream targets fibronectin and smooth muscle actin. Co-IP, ubiquitination assay, RNAi knockdown, phospho-SMAD2/3 western blot, USP11 overexpression, in vivo mouse model Cell death & disease High 27853171
2017 USP11 positively regulates nucleotide excision repair (NER) by deubiquitinating XPC and promoting its retention at UV-induced DNA damage sites. UV irradiation induces USP11 recruitment to chromatin and USP11 interaction with XPC in an XPC-ubiquitination-dependent manner. Co-IP, ubiquitination assay, chromatin fractionation, UV irradiation NER assay, RNAi knockdown Oncotarget Medium 29228550
2018 USP11 was identified as a deubiquitylase that binds p21 in the nucleus, catalyzes removal of polyubiquitin chains conjugated onto p21, and stabilizes p21 protein. USP11 reverses p21 polyubiquitylation mediated by SCFSKP2, CRL4CDT2, and APC/CCDC20 in a cell-cycle-independent manner. Loss of USP11 destabilizes p21, induces G1/S transition, abolishes p21 accumulation after DNA damage, abrogates the G2 checkpoint, and induces apoptosis. USP11-mediated p21 stabilization inhibits cell proliferation and tumorigenesis in vivo. Co-IP, ubiquitination assay, protein half-life analysis, cell cycle analysis, DNA damage response assay, RNAi + rescue, in vivo tumor assay Proceedings of the National Academy of Sciences of the United States of America High 29666278
2018 USP11 forms a complex with eIF4B and deubiquitinates it, stabilizing eIF4B and enhancing its activity. FASN-induced PI3K-S6Kinase signaling phosphorylates USP11, enhancing its interaction with eIF4B and promoting oncogenic translation in DLBCL. Co-IP, mass spectrometry, ubiquitination assay, pharmacological and genetic approaches (kinase inhibitors, RNAi), protein translation assay Nature communications High 29483509
2018 USP11 enhances TGFβ-induced EMT and self-renewal in breast cancer by deubiquitinating and stabilizing TGFβ receptor type II (TGFBR2). USP11 modulation altered TGFβ downstream signaling and metastasis in vivo. USP11 overexpression/knockdown, TGFBR2 ubiquitination assay, Co-IP, TGFβ signaling assays, in vivo metastasis model Molecular cancer research : MCR Medium 29724812
2018 USP11 deubiquitinates RAE1 at the mitotic spindle and functionally modulates RAE1's interaction with NuMA. USP11 knockdown drastically reduces cell proliferation and increases multipolar spindle formation; USP11 is associated with the mitotic spindle and does not regulate spindle assembly checkpoint inactivation. Lentiviral knockdown, ubiquitination assay, co-IP, immunofluorescence (spindle localization), cell proliferation assay, spindle morphology analysis PloS one Medium 29293652
2018 A phage display/next-generation sequencing strategy discovered unique peptide-binding sites in USP11's noncatalytic UBL domain. Crystal structure of a USP11-peptide complex revealed a previously unknown binding site absent in paralogs USP4 and USP15. A binding-pocket-deficient double mutant showed this site modulates USP11's function in homologous recombination-mediated DNA repair. Phage display, next-generation sequencing, isothermal titration calorimetry, X-ray crystallography, HR reporter assay, mutagenesis The Journal of biological chemistry High 30373771
2019 USP11 stabilizes PPP1CA by deubiquitinating it and protecting it from proteasome-mediated degradation. The USP11/PPP1CA complex promotes colorectal cancer progression by activating the ERK/MAPK signaling pathway. LC-MS/MS (protein interaction identification), Co-IP, ubiquitination assay, in vitro and in vivo tumor growth assays, ERK/MAPK signaling readout EBioMedicine Medium 31521612
2019 USP11 acts as a histone deubiquitinase that catalyzes H2AK119 and H2BK120 deubiquitination. USP11 is physically associated with the NuRD chromatin remodeling complex. USP11-mediated histone deubiquitination and NuRD-associated histone deacetylation coordinate to allow timely termination of DNA repair and chromatin reorganization, involving chromatin condensation and genomic stability. Co-IP, in vitro histone deubiquitination assay, chromatin fractionation, DNA repair assay, cell survival assay Nucleic acids research High 31504778
2019 PTEN transcriptionally upregulates USP11 via the PI3K/FOXO pathway. USP11 in turn deubiquitinates and stabilizes PTEN, forming a feedforward regulatory loop. Mice lacking Usp11 display increased susceptibility to PTEN-dependent tumor initiation, growth, and metastasis. Transcriptional reporter assays, Co-IP, ubiquitination assay, Usp11 knockout mice, tumor initiation/growth/metastasis assays Nature communications High 30733438
2020 TRIM32 (E3 ligase) and USP11 (deubiquitinase) antagonistically control ARID1A protein stability. USP11 depletion promotes ARID1A degradation and SCC development; TRIM32 depletion stabilizes ARID1A and inhibits SCC. SDC2 was identified as a downstream target of the ARID1A/USP11 axis. Co-IP, ubiquitination assay, RNAi knockdown/overexpression, cell proliferation/metastasis/chemoresistance assays, epistasis by rescue experiments Cell reports Medium 31914402
2020 RNAi screen of all DUBs in breast cancer cells identified USP11 as a regulator of ERα transcriptional activity. Mass spectrometry revealed changes in proteome and ubiquitinome upon USP11 knockdown in the presence of estradiol. USP11 expression is induced by estradiol (blocked by tamoxifen) in ERα-positive cells. RNAi functional screen, mass spectrometry (proteome/ubiquitinome), RNA sequencing, ERα target gene reporter assays Cancer research Medium 33004351
2021 USP11 promotes autophagy activation by stabilizing Beclin 1 via deubiquitination, which leads to autophagy-dependent ferroptosis after spinal cord ischemia-reperfusion injury. USP11 knockdown in vitro and knockout in vivo decreased neuronal ferroptosis; autophagy inhibition weakened USP11-mediated ferroptosis. Co-IP, ubiquitination assay, siRNA knockdown, USP11 KO mouse model, autophagic flux assay, ferroptosis assays (lipid peroxidation markers), functional recovery assay Cell death and differentiation High 34839355
2021 USP11 deubiquitinates NRF2, stabilizing it and protecting it from proteasome-mediated degradation. USP11 depletion leads to suppression of cell proliferation and induction of ferroptotic cell death via ROS-mediated stress, which is largely abrogated by NRF2 overexpression. Co-IP, ubiquitination assay, RNAi knockdown, NRF2 overexpression rescue, ferroptosis assay (ROS measurement), cell proliferation assay Oncogene High 33531626
2021 USP11 interacts with and deubiquitinates E2F1 to maintain E2F1 protein stability. E2F1 in turn regulates USP11 expression at the transcriptional level, forming a positive E2F1/USP11 feedback loop that promotes HCC cell proliferation, migration, and inhibits autophagy via ERK/mTOR pathway. Co-IP, ubiquitination assay, transcription assay, RNAi + rescue, in vitro and in vivo tumor assays, ERK/mTOR signaling readout Cancer letters Medium 34044068
2021 USP11 deubiquitinates SPRTN metalloprotease, cleaving monoubiquitinated SPRTN in cells and in vitro. USP11 interacts with SPRTN; USP11 depletion impairs SPRTN deubiquitination, promotes SPRTN auto-proteolysis in response to formaldehyde-induced DNA-protein crosslinks (DPCs), and causes accumulation of unrepaired DPCs and hypersensitivity to DPC-inducing agents. Co-IP, in vitro deubiquitination assay, DPC repair assay, DPC accumulation measurement, cell sensitivity assay The Journal of biological chemistry High 33567341
2021 USP11 deubiquitinates senataxin (SETX) — USP11 depletion increases SETX K48-ubiquitination and protein turnover, decreasing steady-state SETX levels and reducing R-loop dissolution. Loss of USP11 reduces SETX enrichment at the KEAP1 promoter, leading to R-loop accumulation and formation of double-strand breaks. KEAP1 opposes USP11 by promoting SETX K48-ubiquitination and degradation. Co-IP, ubiquitination assay (K48-specific), USP11 KO cells, R-loop immunofluorescence/slot blot, SETX ChIP, DSB assay, KEAP1 overexpression Nature communications High 34526504
2021 USP11 coprecipitates with the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PI3KC3-C1) and limits its interaction with nuclear receptor-binding factor 2 (NRBF2), decreasing lipid kinase activity and reducing recruitment of WIPI proteins to the autophagosomal membrane. USP11 also stabilizes mTOR, further restraining autophagy induction. USP11 KO elevates autophagic flux. Co-IP, lipid kinase activity assay, WIPI2 puncta analysis, autophagic flux assay (USP11 KO mammalian cells), C. elegans depletion + amyloid-β protection assay, mTOR stability assay The Journal of biological chemistry High 34600886
2021 Usp11 stabilizes Sox11 protein via deubiquitination in embryonic cerebral cortex. Usp11 deficiency impairs cortical layer 6 neuron production and delays late-born neuronal migration; the disease-associated Usp11 mutant fails to stabilize Sox11. These functions are required for proper cortical neurogenesis and cognition. Usp11 KO mouse, Co-IP, ubiquitination assay, cortical layer analysis, behavioral assays, disease-associated mutant characterization Science advances High 33579706
2021 USP11 deubiquitinates NF90 (nuclear factor 90), protecting it from proteasomal degradation by removing polyubiquitin chains. USP11 and NONO colocalize and interact in the nucleus of melanoma cells; USP11 knockdown decreases NONO levels and inhibits melanoma cell proliferation, which is rescued by NONO overexpression. Co-localization (immunofluorescence), Co-IP, ubiquitination assay, RNAi knockdown + rescue, proliferation assay Journal of cellular and molecular medicine Medium 33369124
2022 Non-mitochondrial HK2 directly interacts with CD133 and promotes recruitment of USP11 to CD133, thereby inhibiting CD133 polyubiquitylation and degradation. USP11 stabilizes CD133 by removing ubiquitin chains, enhancing cancer cell stemness and tumor growth in SCLC. Co-IP, mass spectrometry, in vitro and in vivo ubiquitination assay, flow cytometry, xenograft model, immunofluorescence Cancer communications Medium 35975322
2022 ERK1/2 phosphorylates USP11 at Ser905, promoting cytoplasmic localization of USP11. In the cytoplasm, USP11 colocalizes with and deubiquitinates cytoplasmic p21, stabilizing it and promoting breast cancer cell proliferation in vitro and in vivo. Co-IP, ubiquitination assay, phosphosite mapping (Ser905), ERK1/2 kinase assay, subcellular fractionation, in vitro and in vivo proliferation assays International journal of biological sciences Medium 35414784
2023 USP11 interacts with and deubiquitinates Sirt3 (IP/MS and Co-IP), stabilizing it to reduce oxidative stress-induced ferroptosis. USP11 KO mice exhibit exacerbated intervertebral disc degeneration that is reversed by Sirt3 overexpression, establishing USP11 upstream of Sirt3 in ferroptosis regulation. IP/MS, Co-IP, ubiquitination assay, USP11 KO and Sirt3 KO mice, ferroptosis assay (GPX4, GSH), in vivo rescue experiment, pain behavioral scores Redox biology High 37099926
2023 USP11 interacts with and deubiquitinates eEF1A1 at Lys439, inhibiting its ubiquitin-mediated degradation. Elevated eEF1A1 binds SP1, driving SP1 binding to the HGF gene promoter to increase HGF transcription, activating downstream PI3K/AKT signaling and promoting EMT and metastasis in HCC. Co-IP, ubiquitination assay (site-specific K439 mapping), ChIP assay (SP1 on HGF promoter), PI3K/AKT signaling readout, in vitro and in vivo metastasis assays Oncogene Medium 37973952
2023 USP11 interacts with and stabilizes LSH (lymphoid-specific helicase) by deubiquitination; this interaction is disrupted by erastin treatment. LSH in turn activates transcription of CYP24A1 by binding its promoter and reducing H3K27me3, inhibiting excessive Ca2+ influx and lipid peroxidation to confer ferroptosis resistance in colorectal cancer. Co-IP, ubiquitination assay, ChIP (LSH on CYP24A1 promoter), H3K27me3 analysis, Ca2+ influx assay, lipid peroxidation assay, ferroptosis assay Cell death & disease Medium 37414755
2023 USP11 promotes SMYD5 protein stabilization in HIV-infected T cells. USP11 is required to elevate SMYD5 protein levels when Tat is expressed; loss of USP11 prevents Tat-induced SMYD5 upregulation. SMYD5 methylates Tat in vitro and activates the HIV-1 promoter. Knockdown of USP11, western blotting for SMYD5, in vitro methylation assay (SMYD5 on Tat), HIV-1 transcription assay Cell reports Medium 36897778
2024 USP11 deubiquitinates AR and c-Myc proteins to increase their stability, and also deubiquitinates the repressive histone mark H2A-K119Ub on promoters of AR and c-Myc genes to increase their transcription. Catalytic-inactive USP11 mutant significantly decreased chromatin binding by AR and c-Myc (ChIP-seq). USP11 KD significantly inhibited PCa cell growth. RNAi knockdown, catalytic-dead mutant replacement, RNA-seq, ChIP-seq, ubiquitination assay, Co-IP, rescue with AR/c-Myc re-expression Proceedings of the National Academy of Sciences of the United States of America High 39052835
2024 USP11 deubiquitinates E-cadherin at K738 and maintains luminal differentiation and DNA damage repair in mammary epithelial cells. Usp11 KO mice show reduced E-cadherin expression and increased DNA damage in MECs; WT but not deubiquitinase-inactive USP11 promotes luminal differentiation, DNA damage repair, and suppresses tumorigenesis. Usp11 KO mice, Co-IP, ubiquitination assay (site K738 identified), wild-type vs. catalytic-dead mutant overexpression, luminal differentiation marker analysis, tumor assay The Journal of biological chemistry High 39270819
2024 USP11 deubiquitinates TPIT (a key transcription factor for POMC), enhancing its protein stability, which promotes POMC transcription and ACTH secretion in functioning ACTH pituitary tumors. USP11 knockdown substantially reduces POMC transcription and ACTH secretion in vitro and in vivo. Co-IP, ubiquitination assay, siRNA knockdown, POMC reporter assay, in vivo mouse model, ACTH ELISA Acta neuropathologica communications Medium 39910602
2024 USP11 deubiquitinates OTUD5 and implicates the OTUD5-STING signaling pathway in the inflammatory response of endothelial cells after radiation-induced pneumonitis. Comprehensive proteome and ubiquitinome analyses identified USP11 overexpression as upregulating OTUD5. Comprehensive proteome and ubiquitinome analysis, Co-IP, western blot, Usp11 KO mouse, USP11 inhibitor (mitoxantrone), cytokine measurement International journal of radiation oncology, biology, physics Medium 38364946
2024 DNMT3A stability is maintained by USP11 counteracting CUL4-DCAF8 E3 ligase-mediated degradation. USP11 also enhances DNMT3A SUMOylation by promoting DNMT3A interaction with SUMO E3 ligases and enhances DNMT3A binding to the polycomb complex, maintaining DNMT3A DNA methyltransferase activity. Stable USP11 expression, E1 enzyme inhibition, Co-IP, SUMOylation assay, DNA methyltransferase activity assay, subcellular localization studies bioRxivpreprint Medium 40161590
2024 USP11 deubiquitinates GSK3β, modulates its Ser9 phosphorylation level, and consequently alters its enzymatic activity. USP11 deficiency protects against stress-induced synaptic injury and depressive-like behaviors by restoring synaptic protein expression in the prefrontal cortex via GSK3β/mTOR signaling. IP-MS, Co-IP, ubiquitination assay, phospho-GSK3β analysis, transgenic mice, primary neuronal cultures, behavioral assays (CUMS model) Neurobiology of stress Medium 41889422
2025 The UBL2 domain of USP11 confers selectivity toward K48-linked polyubiquitin chains. In vitro studies with Ub-tetramers and ubiquitinated proteins demonstrate that UBL2 domain endows USP11 with K48 chain preference, an activity not observed in paralogs USP4 and USP15. In vitro ubiquitin chain cleavage assay (Ub-tetramers, ubiquitinated substrates), domain-deletion constructs, cell-based analysis, AI-based virtual screening for inhibitors The Journal of biological chemistry High 41207628
2025 MYCBP2-SPRYD3 E3 ligase complex ubiquitinates USP11 on cysteine 318 (non-canonical ubiquitination), promoting bipolar spindle formation and mitotic slippage in the presence of microtubule-targeting drugs. This demonstrates a DUB-E3 ligase cooperation where the E3 modifies the DUB itself. Co-IP, ubiquitination assay (site-specific Cys318), spindle formation analysis, mitotic slippage assay, anti-microtubule drug treatment The Journal of biological chemistry Medium 41052634
2025 USP11 stabilizes HINT3 via deubiquitination (reversing polyubiquitination), and HINT3 interacts with BCL2. USP11 overexpression increases BCL2 levels, contributing to endothelial apoptosis resistance in pulmonary arterial hypertension. Co-IP, ubiquitination assay, overexpression/knockdown, BCL2 protein level analysis, PAH patient tissue analysis Journal of respiratory biology and translational medicine Medium 40376595
2025 USP11 deubiquitinates Rac1-GTP at residues K123/K147/K183, stabilizing active Rac1. Ionizing radiation (IR) activates Rac1 and induces its nuclear translocation; Rac1-GTP transcriptionally upregulates USP11, forming a self-reinforcing Rac1-USP11 feedback loop driving radioresistance in HCC. Co-IP, ubiquitination assay (site-specific K123/K147/K183), dual luciferase (USP11 promoter), GST pulldown, Rac1-GTP measurement, site-directed mutagenesis, colony formation, xenograft model British journal of cancer High 41298816
2025 USP11 binds to PRDX2 and removes its K63-linked polyubiquitination, promoting PRDX2 translocation into the nucleus. Nuclear PRDX2 interacts with c-MYC, facilitating co-nuclear translocation and enhancing KDR (VEGFR2) transcription, activating VEGFR2 signaling and promoting angiogenesis. Co-IP, ubiquitination assay (K63-specific), USP11 KO in vitro and in vivo (retinal model), subcellular fractionation, ChIP (c-MYC on KDR promoter), angiogenesis assay Angiogenesis Medium 40199774
2025 USP11 interacts with, deubiquitinates, and extends the half-life of SFTPC (surfactant protein C), more potently stabilizing the pathogenic I73T mutant than wild-type. USP11 depletion in hiPSC-derived alveolar organoids mitigates SFTPC-I73T-induced fibrosis; pharmacological inhibition of USP11 prevents fibrosis in TGF-β-treated organoids and bleomycin mouse model. CRISPR/Cas9 genome-wide DUB screen, Co-IP, TUBEs ubiquitination assay, protein half-life assay, hiPSC-derived alveolar organoid model, bleomycin mouse model, mitoxantrone (USP11 inhibitor) treatment Theranostics High 40225577
2025 Usp11 sustains the survival of marginal zone B cells under ionizing radiation by deubiquitinating Notch ligands DLL1 and JAG2, regulating their ubiquitination and thereby promoting immune cell remodeling in the spleen. Co-IP, ubiquitination assay, Usp11 KO mouse, single-cell sequencing, flow cytometry, immunofluorescence, TBI model Cell death & disease Medium 39904982

Source papers

Stage 0 corpus · 96 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2021 USP11 regulates autophagy-dependent ferroptosis after spinal cord ischemia-reperfusion injury by deubiquitinating Beclin 1. Cell death and differentiation 186 34839355
2004 BRCA2 is ubiquitinated in vivo and interacts with USP11, a deubiquitinating enzyme that exhibits prosurvival function in the cellular response to DNA damage. Molecular and cellular biology 139 15314155
2018 Deubiquitylation and stabilization of p21 by USP11 is critical for cell-cycle progression and DNA damage responses. Proceedings of the National Academy of Sciences of the United States of America 136 29666278
2023 The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3. Redox biology 133 37099926
2010 Sensitivity to poly(ADP-ribose) polymerase (PARP) inhibition identifies ubiquitin-specific peptidase 11 (USP11) as a regulator of DNA double-strand break repair. The Journal of biological chemistry 114 20233726
2019 USP11 promotes growth and metastasis of colorectal cancer via PPP1CA-mediated activation of ERK/MAPK signaling pathway. EBioMedicine 109 31521612
2013 Mitoxantrone targets human ubiquitin-specific peptidase 11 (USP11) and is a potent inhibitor of pancreatic cancer cell survival. Molecular cancer research : MCR 104 23696131
2012 USP11 augments TGFβ signalling by deubiquitylating ALK5. Open biology 99 22773947
2014 USP11 regulates PML stability to control Notch-induced malignancy in brain tumours. Nature communications 92 24487962
2010 USP11 negatively regulates TNFalpha-induced NF-kappaB activation by targeting on IkappaBalpha. Cellular signalling 85 19874889
2021 The deubiquitinase USP11 regulates cell proliferation and ferroptotic cell death via stabilization of NRF2 USP11 deubiquitinates and stabilizes NRF2. Oncogene 83 33531626
2002 Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM. The Biochemical journal 77 12084015
2018 Fatty Acid Synthase induced S6Kinase facilitates USP11-eIF4B complex formation for sustained oncogenic translation in DLBCL. Nature communications 75 29483509
2016 Regulation of XIAP Turnover Reveals a Role for USP11 in Promotion of Tumorigenesis. EBioMedicine 67 28040451
2019 USP11 acts as a histone deubiquitinase functioning in chromatin reorganization during DNA repair. Nucleic acids research 65 31504778
2019 PTEN self-regulates through USP11 via the PI3K-FOXO pathway to stabilize tumor suppression. Nature communications 64 30733438
2015 USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics. Cell death and differentiation 63 25613375
2022 A non-metabolic function of hexokinase 2 in small cell lung cancer: promotes cancer cell stemness by increasing USP11-mediated CD133 stability. Cancer communications (London, England) 60 35975322
2007 The deubiquitinating enzyme USP11 controls an IkappaB kinase alpha (IKKalpha)-p53 signaling pathway in response to tumor necrosis factor alpha (TNFalpha). The Journal of biological chemistry 59 17897950
2015 USP11 Is a Negative Regulator to γH2AX Ubiquitylation by RNF8/RNF168. The Journal of biological chemistry 57 26507658
2016 De-ubiquitinating enzyme, USP11, promotes transforming growth factor β-1 signaling through stabilization of transforming growth factor β receptor II. Cell death & disease 55 27853171
2015 Evolution of the highly networked deubiquitinating enzymes USP4, USP15, and USP11. BMC evolutionary biology 54 26503449
2008 USP11 stabilizes HPV-16E7 and further modulates the E7 biological activity. The Journal of biological chemistry 52 18408009
2014 USP11 regulates p53 stability by deubiquitinating p53. Journal of Zhejiang University. Science. B 51 25471832
2018 USP11 Enhances TGFβ-Induced Epithelial-Mesenchymal Plasticity and Human Breast Cancer Metastasis. Molecular cancer research : MCR 50 29724812
2020 TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma. Cell reports 47 31914402
2021 The E2F1/USP11 positive feedback loop promotes hepatocellular carcinoma metastasis and inhibits autophagy by activating ERK/mTOR pathway. Cancer letters 43 34044068
2015 Ubiquitin-specific Protease 11 (USP11) Deubiquitinates Hybrid Small Ubiquitin-like Modifier (SUMO)-Ubiquitin Chains to Counteract RING Finger Protein 4 (RNF4). The Journal of biological chemistry 36 25969536
2018 Upregulation of USP11 promotes epithelial‑to‑mesenchymal transition by deubiquitinating Snail in ovarian cancer. Oncology reports 35 30569152
2021 Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization. Science advances 34 33579706
2016 Ubiquitin-specific protease 11 (USP11) functions as a tumor suppressor through deubiquitinating and stabilizing VGLL4 protein. American journal of cancer research 32 28042509
2023 USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1. Cell death & disease 31 37414755
2018 Discovery of peptide ligands targeting a specific ubiquitin-like domain-binding site in the deubiquitinase USP11. The Journal of biological chemistry 30 30373771
2021 USP11 degrades KLF4 via its deubiquitinase activity in liver diseases. Journal of cellular and molecular medicine 27 34114341
2021 A pro-inflammatory mediator USP11 enhances the stability of p53 and inhibits KLF2 in intracerebral hemorrhage. Molecular therapy. Methods & clinical development 26 34141823
2021 USP11 controls R-loops by regulating senataxin proteostasis. Nature communications 26 34526504
2022 Deubiquitinating enzyme USP11 promotes renal tubular cell senescence and fibrosis via inhibiting the ubiquitin degradation of TGF-β receptor II. Acta pharmacologica Sinica 25 36045219
2021 USP11 facilitates colorectal cancer proliferation and metastasis by regulating IGF2BP3 stability. American journal of translational research 25 33594305
2021 Circ_DOCK1 regulates USP11 through miR-132-3p to control colorectal cancer progression. World journal of surgical oncology 25 33685455
2020 Aberrant USP11 expression regulates NF90 to promote proliferation and metastasis in hepatocellular carcinoma. American journal of cancer research 25 32509388
2020 A Functional Genomic Screen Identifies the Deubiquitinase USP11 as a Novel Transcriptional Regulator of ERα in Breast Cancer. Cancer research 25 33004351
2023 USP11 potentiates HGF/AKT signaling and drives metastasis in hepatocellular carcinoma. Oncogene 22 37973952
2017 Regulation of XPC deubiquitination by USP11 in repair of UV-induced DNA damage. Oncotarget 22 29228550
2021 USP11 mediates repair of DNA-protein cross-links by deubiquitinating SPRTN metalloprotease. The Journal of biological chemistry 21 33567341
2021 USP11 induce resistance to 5-Fluorouracil in Colorectal Cancer through activating autophagy by stabilizing VCP. Journal of Cancer 20 33758608
2018 USP11 deubiquitinates RAE1 and plays a key role in bipolar spindle formation. PloS one 20 29293652
2024 DLGAP5 triggers proliferation and metastasis of bladder cancer by stabilizing E2F1 via USP11. Oncogene 19 38182895
2024 USP11 promotes prostate cancer progression by up-regulating AR and c-Myc activity. Proceedings of the National Academy of Sciences of the United States of America 19 39052835
2015 USP11, Deubiquitinating Enzyme, Associated with Neuronal Apoptosis Following Intracerebral Hemorrhage. Journal of molecular neuroscience : MN 18 26334325
2022 The Dual Role of USP11 in Cancer. Journal of oncology 16 35359344
2020 The regulation of NONO by USP11 via deubiquitination is linked to the proliferation of melanoma cells. Journal of cellular and molecular medicine 16 33369124
2024 USP11 promotes glycolysis by regulating HIF-1α stability in hepatocellular carcinoma. Journal of cellular and molecular medicine 15 38229475
2024 USP11 Exacerbates Radiation-Induced Pneumonitis by Activating Endothelial Cell Inflammatory Response via OTUD5-STING Signaling. International journal of radiation oncology, biology, physics 14 38364946
2023 The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11. Cell reports 14 36897778
2022 The RSL3 Induction of KLK Lung Adenocarcinoma Cell Ferroptosis by Inhibition of USP11 Activity and the NRF2-GSH Axis. Cancers 13 36358651
2020 CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs). Biochemical and biophysical research communications 13 32703400
2021 Inhibition of USP11 sensitizes gastric cancer to chemotherapy via suppressing RhoA and Ras-mediated signaling pathways. Clinics and research in hepatology and gastroenterology 12 34332125
2024 USP11 regulates proliferation and apoptosis of human spermatogonial stem cells via HOXC5-mediated canonical WNT/β-catenin signaling pathway. Cellular and molecular life sciences : CMLS 11 38722330
2024 USP11 promotes lipogenesis and tumorigenesis by regulating SREBF1 stability in hepatocellular carcinoma. Cell communication and signaling : CCS 10 39558331
2024 The USP11/Nrf2 positive feedback loop promotes colorectal cancer progression by inhibiting mitochondrial apoptosis. Cell death & disease 10 39617751
2021 The deubiquitinase USP11 is a versatile and conserved regulator of autophagy. The Journal of biological chemistry 10 34600886
2021 Targeting USP11 may alleviate radiation-induced pulmonary fibrosis by regulating endothelium tight junction. International journal of radiation biology 10 34705600
2024 USP11 promotes renal tubular cell pyroptosis and fibrosis in UUO mice via inhibiting KLF4 ubiquitin degradation. Acta pharmacologica Sinica 9 39147900
2023 USP11 exacerbates neuronal apoptosis after traumatic brain injury via PKM2-mediated PI3K/AKT signaling pathway. Brain research 9 36898475
2024 Targeting USP11 regulation by a novel lithium-organic coordination compound improves neuropathologies and cognitive functions in Alzheimer transgenic mice. EMBO molecular medicine 8 39394468
2024 The deubiquitinating enzyme USP11 regulates breast cancer progression by stabilizing PGAM5. Breast cancer research : BCR 7 39300548
2022 ERK-mediated Cytoplasmic Retention of USP11 Contributes to Breast Cancer Cell Proliferation by Stabilizing Cytoplasmic p21. International journal of biological sciences 7 35414784
2015 USP11: A key regulator of cIAP2 stability and sensitivity to SMAC mimetics. Molecular & cellular oncology 7 27314066
2023 USP11 plays a critical role in the onset and progression of acute graft-versus-host disease：Novel target for precision therapeutics. Pharmacological research 6 36822452
1998 UHX1 and PCTK1: precise characterisation and localisation within a gene-rich region in Xp11.23 and evaluation as candidate genes for retinal diseases mapped to Xp21.1-p11.2. European journal of human genetics : EJHG 6 9801870
2024 RRM1 promotes homologous recombination and radio/chemo-sensitivity via enhancing USP11 and E2F1-mediated RAD51AP1 transcription. Cell death discovery 5 39695160
2025 Endothelial USP11 drives VEGFR2 signaling and angiogenesis via PRDX2/c-MYC axis. Angiogenesis 4 40199774
2024 USP11 deubiquitinates E-cadherin and maintains the luminal fate of mammary tumor cells to suppress breast cancer. The Journal of biological chemistry 4 39270819
2024 USP11 modulates mitotic progression and senescence by regulating the p53-p21 axis through MDM2 deubiquitination. Biochemical and biophysical research communications 3 38901057
2025 X-linked ubiquitin-specific peptidase 11 (USP11) increases susceptibility to Cushing's disease in women. Acta neuropathologica communications 2 39910602
2025 Targeting USP11 counteracts SFTPC -associated interstitial lung disease in hiPSCs-derived alveolar organoids and in vivo models. Theranostics 2 40225577
2025 'Nelfinavir sensitizes a clinically relevant chemo-radioresistant cervical cancer in-vitro model by targeting the AKT-USP15/USP11-HPV16 E6/E7 axis. Biochemistry and biophysics reports 2 40242402
2025 USP11-PGAM5 Axis Promotes Neurotoxic Astrocyte Reactivity by Aggravating the mtDNA-cGAS-STING Pathway After Intracerebral Hemorrhage. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2 41201111
2025 Usp11 maintained the survival of marginal zone B cells under ionizing radiation by deubiquitinating DLL1 and JAG2. Cell death & disease 1 39904982
2025 USP11 promotes autophagy to attenuate LPS-induced oxidative stress in lung epithelial cells by stabilizing FOXO1 levels. Biochemical and biophysical research communications 1 39908907
2025 Stability and DNA Methyltransferase Activity of DNMT3A are Maintained by Ubiquitin-Specific Peptidase 11 (USP11) and Sumoylation Countering Degradation. bioRxiv : the preprint server for biology 1 40161590
2025 Identification of chemical scaffolds for targeting ubiquitin-specific protease 11 (USP11) through high-throughput virtual screening. Journal of enzyme inhibition and medicinal chemistry 1 40588719
2025 Inhibition of USP11 attenuates sepsis-associated acute kidney injury by downregulating TGFBR2/Smad3 signaling. Frontiers in molecular biosciences 1 40656894
2025 Sevoflurane suppresses LPS-induced microglia injury by regulating USP11-mediated UHRF1 deubiquitination. Regenerative therapy 1 41159065
2025 USP11 promotes colorectal cancer progression by stabilizing EGFR and TRAF6: a potential therapeutic target in EGFR- and TLR-driven tumorigenesis. Cell death & disease 1 41419456
2026 Golgi fragmentation driven by the USP11-ITCH axis triggers autolysosomal failure in neurodegeneration. Autophagy 0 41655130
2026 USP11 drives stress-induced synaptic structural deficits and depression-like behaviors through GSK3β/mTOR signaling. Neurobiology of stress 0 41889422
2025 USP11 Promotes Endothelial Apoptosis-Resistance in Pulmonary Arterial Hypertension by Deubiquitinating HINT3. Journal of respiratory biology and translational medicine 0 40376595
2025 Mechanism of USP11 regulated SIRT3/ROS in drug resistance of acute myeloid leukemia. Clinical and experimental medicine 0 40736712
2025 USP11 stabilizes RALY to promote FXYD5-mediated aerobic glycolysis and aggravate pancreatic cancer progression. Experimental cell research 0 40972883
2025 USP11 is involved in the sensitivity of liver cancer cells to ferroptosis and taxanes through the regulation of NRF2 ubiquitin-mediated degradation. Translational oncology 0 41033073
2025 The E3 ubiquitin ligase SPRYD3-MYCBP2(PAM) regulates mitotic cell fate and ubiquitination of USP11 to control spindle assembly. The Journal of biological chemistry 0 41052634
2025 Non-catalytic UBL2 domain directs deubiquitinase USP11 toward K48-linked polyubiquitin chains. The Journal of biological chemistry 0 41207628
2025 The Rac1-USP11 feedback amplification loop: a radiation-activated engine driving radioresistance in hepatocellular carcinoma. British journal of cancer 0 41298816
2025 Forsythiaside A ameliorates acetaminophen-induced liver injury by suppressing ferroptosis through activating USP11/SIRT3-mediated mitophagy. Journal of ethnopharmacology 0 41338315
2025 Discovery, development, and characterization of potent and selective USP11 inhibitors. Pharmacological research 0 41461277