Affinage

SELPLG

P-selectin glycoprotein ligand 1 · UniProt Q14242

Length
412 aa
Mass
43.2 kDa
Annotated
2026-06-10
100 papers in source corpus 50 papers cited in narrative 50 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PSGL-1 (SELPLG/CD162) is a dimeric, tyrosine-sulfated, O-glycosylated mucin-like leukocyte receptor that serves as the principal ligand for P-, E-, and L-selectins to mediate the tethering and rolling steps of leukocyte recruitment to inflamed and lymphoid tissue (PMID:11081633, PMID:10601352, PMID:15880112). High-affinity selectin binding requires the cooperative action of N-terminal sulfated tyrosines and core-2 sialyl Lewis X glycans on the PSGL-1 N-terminus: tyrosine sulfation is essential for P-selectin adhesion, Tyr-48 versus Tyr-51 discriminate P- from L-selectin engagement, and FucT-VII-dependent core-2 O-glycans on Thr-57 are critical for L- and P-selectin rolling (PMID:11081633, PMID:7585950, PMID:12403782, PMID:15579466). Dimerization through the extracellular cysteine C320 is required specifically for P-selectin recognition (PMID:9660879). Coupling of the PSGL-1 cytoplasmic tail to the actin cytoskeleton via moesin/ERM proteins—through an ERM-binding sequence centered on Arg-337/Lys-338 and a β-strand interaction with the FERM C-subdomain—stabilizes rolling on selectins (PMID:12036880, PMID:18076570, PMID:22311979). Beyond adhesion, selectin or chemokine engagement of PSGL-1 initiates outside-in signaling: it nucleates a Syk/SFK (Fgr, Hck, Lyn) → DAP12/FcRγ ITAM → BTK → p38 MAPK cascade that activates the integrin LFA-1 to convert fast rolling into slow rolling, a function that requires the PSGL-1 cytoplasmic domain, and a constitutive PSGL-1–L-selectin complex amplifies this ITAM signaling output (PMID:12387735, PMID:18550846, PMID:18794338, PMID:20299514, PMID:24127491). PSGL-1 cooperates with CXCR2 to drive β2-integrin-dependent neutrophil arrest, NET release, and thrombosis (PMID:30068506), and downstream signaling additionally controls macrophage chemotaxis via Akt/mTOR-dependent ROCK-1 translation (PMID:17245434). In adaptive immunity PSGL-1 functions as an inhibitory checkpoint that restrains TCR signaling by limiting Zap70 phosphorylation and maintaining the Zap70 inhibitor Sts-1, upregulating PD-1 and driving CD8+ T cell exhaustion, and it is engaged by VISTA at acidic pH to suppress T cells in the tumor microenvironment (PMID:27192578, PMID:37115668, PMID:31645726). PSGL-1 also acts as a broad-spectrum antiviral restriction factor: incorporated into HIV-1, coronavirus, and other virions, it sterically blocks virion attachment to target cells via its ectodomain, an activity countered by HIV-1 Vpu, which recruits SCFβ-TrCP2 to ubiquitinate and degrade PSGL-1 (PMID:30833724, PMID:32273392, PMID:32193343). Conversely, PSGL-1 serves as an entry receptor for enterovirus 71 via a VP1-145 capsid switch and caveolar endocytosis (PMID:23935488, PMID:23760234).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1995 High

    Established the chemical determinant of selectin binding by showing PSGL-1's function depends on a post-translational modification rather than its peptide backbone alone.

    Evidence Tyr-to-Phe mutagenesis, sulfation inhibitors, and HL-60 rolling assays on P-selectin

    PMID:7585950

    Open questions at the time
    • Did not resolve how sulfate and glycan jointly contact the lectin domain
    • Did not distinguish P- versus L-selectin tyrosine requirements
  2. 1996 Medium

    Extended PSGL-1's ligand repertoire beyond P-selectin by identifying it as an L-selectin ligand mediating neutrophil homotypic aggregation.

    Evidence Anti-PSGL-1 Fab and dual L-selectin/PSGL-1 antibody blockade in flow-cytometry aggregation assays

    PMID:8839831

    Open questions at the time
    • Antibody blockade only, no genetic confirmation
    • Single lab
  3. 1998 High

    Defined dimerization as a structural prerequisite for P-selectin recognition, distinguishing it from mere surface expression.

    Evidence C320A cysteine mutagenesis with native/denaturing Western blot and rolling assays in K562 transfectants

    PMID:9660879

    Open questions at the time
    • Whether dimerization is required for E- and L-selectin binding unresolved
    • Stoichiometry of the dimer-selectin complex not determined
  4. 1999 High

    Demonstrated in vivo that PSGL-1 is essential for P-selectin-dependent early neutrophil rolling but dispensable for late E-selectin rolling, revealing selectin-specific and temporally distinct roles.

    Evidence PSGL-1 knockout mice with intravital microscopy of cremaster venules and peritonitis models

    PMID:10601352

    Open questions at the time
    • Did not identify the alternative E-selectin ligands compensating in late inflammation
  5. 2000 High

    Provided the atomic-resolution basis for how sulfated tyrosines and sialyl Lewis X cooperate at the P-selectin interface.

    Evidence X-ray crystal structure of P-selectin lectin-EGF domains bound to a modified PSGL-1 N-terminal peptide

    PMID:11081633

    Open questions at the time
    • Structure of full-length dimeric PSGL-1 not solved
    • E- and L-selectin complexes not crystallized
  6. 2002 High

    Connected PSGL-1 adhesion to intracellular signaling by showing it recruits Syk through ERM proteins and that cytoskeletal anchorage via moesin stabilizes rolling.

    Evidence Reciprocal Co-IP, dominant-negative ITAM-mutant moesin and dead-kinase Syk, SRE reporter, and rolling assays with truncated PSGL-1

    PMID:12036880 PMID:12387735

    Open questions at the time
    • The ITAM adaptors bridging Syk were not yet identified
    • Moesin selectivity over other ERM proteins mechanistically unexplained
  7. 2008 High

    Defined the ITAM-dependent slow-rolling pathway, identifying Fgr, DAP12, and FcRγ as the adaptors converting selectin engagement into LFA-1 activation independently of chemokine receptors.

    Evidence ΔCD knock-in and fgr/Tyrobp/Fcrg-deficient mice with density-matched rolling assays and Syk/p38 phosphorylation readouts

    PMID:18550846 PMID:18794338

    Open questions at the time
    • The intermediate kinase between Syk and p38 was not yet defined
    • Whether the cytoplasmic-tail requirement reflects ITAM nucleation or cytoskeletal coupling left open
  8. 2010 High

    Placed BTK as the key intermediate between Syk and p38 in the shared E-selectin slow-rolling pathway used by PSGL-1 and CD44.

    Evidence Hck/Lyn/Fgr-deficient mice, lipid-raft disruption, and kinase inhibitors with intravital microscopy

    PMID:20299514

    Open questions at the time
    • How lipid rafts spatially organize the cascade not fully resolved
  9. 2013 High

    Revealed a constitutive PSGL-1–L-selectin signaling complex and CXCR2 cooperativity as amplifiers driving integrin-dependent arrest and thrombosis.

    Evidence Endogenous Co-IP, L-selectin cytoplasmic mutants, SLP-76 phosphosite mapping, and IVC flow-restriction intravital models

    PMID:24127491 PMID:30068506

    Open questions at the time
    • Stoichiometry and assembly trigger of the PSGL-1–L-selectin complex not defined
  10. 2016 High

    Reframed PSGL-1 as an inhibitory immune checkpoint by showing its ligation suppresses TCR/IL-2 signaling and drives CD8+ T cell exhaustion.

    Evidence PSGL-1 knockout mice in chronic LCMV and melanoma models with TCR/IL-2 signaling and survival assays

    PMID:27192578

    Open questions at the time
    • The physiological ligand engaging PSGL-1 on exhausted T cells was not identified
    • Upstream biochemical mechanism of TCR attenuation undefined at this stage
  11. 2019 High

    Identified VISTA as a pH-selective PSGL-1 binding partner suppressing T cells in the acidic tumor microenvironment, and uncovered PSGL-1 as an antiviral restriction factor degraded by HIV-1 Vpu.

    Evidence VISTA histidine mutagenesis with pH-selective antibodies; mass-spectrometry, Co-IP, ubiquitination, and HIV infectivity assays in primary CD4+ T cells

    PMID:30833724 PMID:31645726

    Open questions at the time
    • Whether VISTA is the sole inhibitory ligand for PSGL-1 unknown
    • Mechanistic link between checkpoint and antiviral functions unexplored
  12. 2023 High

    Resolved the proximal biochemical mechanism of PSGL-1 checkpoint activity, showing it restrains Zap70 phosphorylation and maintains Sts-1 upstream of PD-1, requiring TCR co-ligation.

    Evidence PSGL-1 knockout mice, Zap70 phosphorylation and Sts-1 expression assays, co-ligation experiments, and tumor models with PSGL-1 blockade

    PMID:37115668

    Open questions at the time
    • How PSGL-1 physically couples to the proximal TCR machinery not defined
    • The endogenous co-ligand on tumor cells not identified
  13. 2020 Medium

    Established the steric mechanism of PSGL-1 antiviral restriction and its breadth across enveloped viruses.

    Evidence HIV domain-deletion infectivity and attachment assays, transinfection assays, F-actin and gp41 binding assays, and coronavirus pseudovirus assays

    PMID:32193343 PMID:32273392 PMID:32802403 PMID:33396594

    Open questions at the time
    • Relative contribution of ectodomain steric blockade versus actin/gp41 mechanisms not quantified
    • Some mechanisms shown in single labs

Open questions

Synthesis pass · forward-looking unresolved questions
  • How PSGL-1's distinct functions—selectin adhesion, integrin-activating ITAM signaling, T-cell checkpoint inhibition, and antiviral virion incorporation—are coordinated or switched in a single cell remains unresolved.
  • No unifying structural model links adhesion and checkpoint functions
  • The physiological PSGL-1 ligand driving T cell exhaustion is undefined
  • Regulation of PSGL-1 surface density by shedding and trafficking is incompletely mapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098631 cell adhesion mediator activity 4 GO:0098772 molecular function regulator activity 4 GO:0008092 cytoskeletal protein binding 3 GO:0001618 virus receptor activity 2
Localization
GO:0005856 cytoskeleton 3 GO:0005886 plasma membrane 3 GO:0005768 endosome 1
Pathway
R-HSA-162582 Signal Transduction 4 R-HSA-1643685 Disease 4 R-HSA-168256 Immune System 4 R-HSA-109582 Hemostasis 3 R-HSA-1500931 Cell-Cell communication 3
Partners
ADAM8MSNSELESELLSELPSNX20SYKVSIR
Complex memberships
PSGL-1–L-selectin signaling complex

Evidence

Reading pass · 50 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 Crystal structure of P-selectin lectin-EGF domains co-complexed with the N-terminal domain of human PSGL-1 modified by tyrosine sulfation and sialyl Lewis X revealed the molecular basis of high-affinity P-selectin/PSGL-1 interaction, showing how sulfated tyrosines and glycan cooperate for binding. X-ray crystallography (crystal structure of P-selectin LE domains with PSGL-1 N-terminal peptide) Cell High 11081633
1995 Tyrosine sulfation of a consensus motif within the N-terminal ~20 residues of PSGL-1 is essential for P-selectin binding; mutation of N-terminal tyrosines to phenylalanine abolishes P-selectin adhesion, and sulfation inhibitors block HL-60 rolling on P-selectin. Site-directed mutagenesis, sulfation inhibitors, cell rolling assay on P-selectin-coated coverslips Cell High 7585950
1998 Dimerization of PSGL-1 through a single conserved extracellular cysteine (C320) is essential for functional recognition of P-selectin; monomeric C320A mutant fails to support rolling or binding to P-selectin chimeras despite intact surface expression. Cysteine-to-alanine mutagenesis, Western blot under native/denaturing conditions, low-shear adhesion and flow-chamber rolling assays in stable K562 transfectants The Journal of cell biology High 9660879
1999 PSGL-1 is required for P-selectin-mediated neutrophil rolling and leukocyte recruitment in early inflammation (30 min post-trauma); however, it is not required for E-selectin-mediated rolling 2 h after TNF-α stimulation, as shown in PSGL-1 gene-knockout mice. Targeted gene disruption (PSGL-1 knockout mice), intravital microscopy of cremaster venules, chemical peritonitis model The Journal of experimental medicine High 10601352
2002 PSGL-1 associates with Syk kinase through ERM proteins (ezrin/moesin); engagement of PSGL-1 induces Syk tyrosine phosphorylation and SRE-dependent transcriptional activation. ITAM-mutated moesin or Syk dead-kinase mutant abrogates PSGL-1-induced transcription. Co-immunoprecipitation, dominant-negative overexpression, pharmacological Syk inhibition (piceatannol), SRE reporter assay Immunity High 12387735
2002 Attachment of the PSGL-1 cytoplasmic domain to the actin cytoskeleton via moesin (but not other ERM proteins) is essential for leukocyte rolling on P-selectin; cytoplasmic truncation abolishes rolling without affecting P-selectin binding or surface distribution of PSGL-1. Stable transfection with truncated PSGL-1 (ΔCyto), actin cytoskeletal toxin treatment, co-immunoprecipitation with ERM proteins, rolling assay in flow chamber Blood High 12036880
2008 The PSGL-1 cytoplasmic domain is dispensable for leukocyte rolling on P-selectin (when PSGL-1 surface density is matched) but is essential for Syk-dependent activation of LFA-1 (αLβ2) to convert fast rolling to slow rolling on ICAM-1. ΔCD knock-in mice expressing cytoplasmic-domain-deleted PSGL-1, O-sialoglycoprotein endopeptidase titration to match PSGL-1 density, intravital microscopy, Syk phosphorylation assay Blood High 18550846
2008 E-selectin engagement of PSGL-1 activates Syk and p38 MAPK to induce slow neutrophil rolling via an ITAM-dependent pathway requiring Src-family kinase Fgr and ITAM adaptor proteins DAP12 and FcRγ, independently of Gαi-coupled receptors. Gene-deficient mice (fgr−/−, Tyrobp−/− Fcrg−/−), flow chamber rolling assay, intravital microscopy, Syk/p38 phosphorylation assay, mixed bone marrow chimeras, peritonitis model The Journal of experimental medicine High 18794338
2010 E-selectin engagement of either PSGL-1 or CD44 triggers slow rolling through a common lipid-raft-dependent pathway using SFKs Hck, Lyn, and Fgr; Bruton's tyrosine kinase (BTK) acts as a key intermediate between Syk and p38. PSGL-1's cytoplasmic domain is required for SFK activation during this process. Gene-deficient mice, flow chamber and intravital microscopy, lipid raft disruption, kinase inhibitors, phosphorylation assays Blood High 20299514
2007 Among E-selectin ligands on neutrophils, PSGL-1 plays the major role in initial leukocyte capture, ESL-1 is critical for converting initial tethers to slow rolling, and CD44 controls rolling velocity and mediates E-selectin-dependent redistribution of PSGL-1 and L-selectin to the major pole via p38 signaling. Gene-targeted and RNA-targeted loss-of-function in mice, intravital microscopy, flow chamber assay Immunity High 17442598
1996 PSGL-1 on neutrophils serves as a ligand for L-selectin, supporting L-selectin–PSGL-1-mediated neutrophil–neutrophil aggregation as the first step in homotypic aggregation. Anti-PSGL-1 Fab inhibition, simultaneous antibody blocking of L-selectin and PSGL-1 on separate cell populations, flow cytometry aggregation assay Blood Medium 8839831
1997 PSGL-1 is the sole P-selectin-binding glycoprotein on Th1 cells (not Th2 cells) despite similar surface expression levels; PSGL-1 on Th1 supports P-selectin binding and partially mediates their migration into cutaneous delayed-type hypersensitivity lesions. Affinity isolation with P-selectin-Ig fusion protein, flow cytometry binding assay, antibody blocking in cell adhesion assay and in vivo DTH model The Journal of experimental medicine High 9053457
1999 PSGL-1 engagement by P-selectin on activated platelets triggers tyrosine kinase-dependent (genistein-sensitive) phosphorylation of a 110 kDa protein and activates CD11b/CD18 (β2 integrin) on PMNs; a non-adhesion-blocking anti-PSGL-1 antibody alone triggers the same β2-integrin-dependent aggregation. Mixed-cell shear-flow aggregation assay, antibody blocking (anti-P-selectin, anti-CD11b/CD18, anti-PSGL-1), tyrosine kinase inhibitors (genistein), tyrosine phosphorylation assay, CHO-P transfectants Blood High 9920836
2013 A subset of PSGL-1 molecules is constitutively associated with L-selectin on neutrophils; this PSGL-1–L-selectin complex signals through Src family kinases and ITAM adaptors to activate LFA-1, and requires the L-selectin cytoplasmic tail for signaling output. Co-immunoprecipitation of endogenous PSGL-1 and L-selectin, L-selectin cytoplasmic tail mutants, flow chamber rolling assay, intravital microscopy, SFK inhibitors The Journal of experimental medicine High 24127491
2007 PSGL-1 signals through Akt/mTOR upon macrophage adherence to promote translational upregulation of ROCK-1 (but not ROCK-2) mRNA, which is required for macrophage chemotaxis and phagocytosis; PSGL-1-deficient macrophages phenocopy mTOR inhibition. PSGL-1−/− macrophages, rapamycin treatment, ribosome loading assay, ROCK-1/2 Western blot, chemotaxis and phagocytosis assays The EMBO journal High 17245434
2016 PSGL-1 ligation on exhausted CD8+ T cells inhibits TCR and IL-2 signaling and upregulates PD-1, leading to diminished survival upon TCR stimulation. PSGL-1-deficient mice clear chronic virus due to intrinsically improved effector T cell survival with reduced inhibitory receptor expression. PSGL-1−/− mice in chronic LCMV infection model, TCR/IL-2 signaling assays, PD-1 expression measurement, T cell survival assays, melanoma tumor model Immunity High 27192578
2023 PSGL-1 attenuates TCR signaling upstream of PD-1 by directly restraining Zap70 phosphorylation and maintaining expression of the Zap70 inhibitor Sts-1; PSGL-1 requires co-ligation with TCR to suppress CD8+ T cell activation and drive terminal exhaustion. PSGL-1−/− mice, Zap70 phosphorylation assays, Sts-1 expression analysis, co-ligation experiments, tumor models with pharmacologic PSGL-1 blockade Cell reports High 37115668
2019 VISTA binds PSGL-1 selectively at acidic pH through multiple histidine residues along the rim of the VISTA extracellular domain; this interaction suppresses T cells in the tumor microenvironment and can be blocked by pH-selective antibodies. Binding assays, site-directed mutagenesis of VISTA histidines, pH-selective blocking antibodies, in vivo immune suppression assays Nature High 31645726
2019 HIV-1 Vpu binds PSGL-1 and induces its ubiquitination and degradation through the ubiquitin ligase SCFβ-TrCP2; PSGL-1 (induced by IFN-γ) inhibits HIV-1 reverse transcription and blocks virion infectivity by incorporating into progeny virions. Isobaric-tag mass spectrometry proteomics, co-immunoprecipitation, ubiquitination assay, siRNA knockdown, HIV infectivity assays, reverse transcription assay in primary CD4+ T cells Nature microbiology High 30833724
2020 Virion-incorporated PSGL-1 blocks HIV-1 infectivity by preventing attachment of virions to target cells, independently of the viral glycoprotein; the extracellular N-terminal domain of PSGL-1 is necessary, and the cytoplasmic tail contributes to inhibition. Vpu (and Nef) down-regulate PSGL-1 from the cell surface to partially escape this restriction. HIV infectivity assays with PSGL-1 domain deletion mutants, particle attachment assays to CD4+ cells, Vpu/Nef expression, murine leukemia virus and influenza A virus infectivity assays Proceedings of the National Academy of Sciences of the United States of America High 32193343 32273392
2020 Virion incorporation of PSGL-1 and CD43 inhibits HIV-1 attachment to both CD4+ target cells and fibroblastic reticular cells mediating transinfection; the full-length ectodomain of PSGL-1 is required, consistent with steric blockade of virus-cell contact. HIV-1 particle attachment assays, PSGL-1 ectodomain truncation mutants, CD4− cell transinfection assay, Gag copatching analysis Proceedings of the National Academy of Sciences of the United States of America High 32193343
2020 PSGL-1 directly binds F-actin to restrict actin dynamics, inhibiting HIV DNA synthesis; incorporated PSGL-1 also binds gp41 and sequesters it at the plasma membrane, reducing Env incorporation into virions and impairing viral entry. F-actin co-sedimentation/binding assay, cryo-EM and super-resolution imaging of Env on virions, gp41 co-immunoprecipitation, HIV DNA synthesis assay Cell discovery Medium 32802403
2007 Crystal structure of the radixin FERM domain complexed with a PSGL-1 juxtamembrane peptide reveals that PSGL-1 binds FERM subdomain C in a beta-strand mode; non-conserved large residues (Met9, His8) compensate for the absence of the canonical Motif-1 alanine, providing structural basis for PSGL-1–ERM interaction. X-ray crystallography of radixin FERM–PSGL-1 peptide complex Genes to cells High 18076570
2004 Human FucT-VII plays the predominant role in generating selectin-binding carbohydrate ligands on PSGL-1, while FucT-IV also contributes; core-2 O-glycans attached to Thr-57 are critical for L- and P-selectin rolling, whereas E-selectin uses additional PSGL-1 binding sites (>75% of rolling is Thr-57-independent). CHO cell transfections with FucT-IV and/or FucT-VII plus core-2 GlcNAcT, PSGL-1 Thr-57 and Thr-44 alanine mutants, rolling adhesion assays on L-, P-, and E-selectin The Journal of biological chemistry High 15579466
2002 For L-selectin binding, Tyr-51 at the PSGL-1 N-terminus plays a predominant role (contrasting with P-selectin where Tyr-48 is key); core-2 O-glycans on Thr-57 are critical for optimal L-selectin binding and for controlling rolling velocity. Tyr-to-Phe and Thr-to-Ala mutagenesis, CHO cell expression with core-2 GlcNAcT and FucT-VII, L-selectin binding assays, rolling adhesion assays, molecular modeling The Journal of biological chemistry High 12403782
2000 PSGL-1 is expressed on platelets at 25–100-fold lower levels than leukocytes; platelet PSGL-1 is functional and mediates platelet rolling in mesenteric venules, as shown by anti-PSGL-1 antibody blockade in intravital microscopy. P-selectin-IgG affinity purification, Western blot, anti-PSGL-1 immunopurification, flow cytometry, intravital microscopy with antibody blockade The Journal of experimental medicine High 10770806
2005 P-selectin and its ligand PSGL-1 mediate recruitment of adult lymphoid progenitors to the thymus; PSGL-1−/− thymi contain fewer early thymic progenitors, and thymic P-selectin expression is regulated by niche occupancy, suggesting a homeostatic P-selectin/PSGL-1 thymic homing axis. Parabiosis, competitive thymus reconstitution, short-term homing assays, PSGL-1−/− mice Nature immunology High 15880112
1999 PSGL-1 ligation on human CD34+ hematopoietic progenitor cells (the sole P-selectin receptor on these cells) by immobilized or soluble P-selectin, or by anti-PSGL-1 antibody, profoundly suppresses HPC proliferation stimulated by growth factors. P-selectin-IgG affinity purification to identify PSGL-1 as the sole receptor, immobilized/soluble ligand and antibody ligation, colony-forming proliferation assays Immunity High 10514015
2013 Engagement of PSGL-1 and CXCR2 on rolling neutrophils cooperatively propagates signals to induce β2 integrin-dependent arrest in flow-restricted inferior vena cava. PSGL-1 signaling in this context uses Tyr-145 (not Tyr-112/128) of SLP-76 and does not require L-selectin; cooperative PSGL-1/CXCR2 signaling increases thrombus frequency and size partly by stimulating NET release. Genetically engineered mice, spinning-disk intravital microscopy, ultrasonography, IVC flow restriction model, pharmacological PSGL-1/CXCR2 blockade, NET quantification Blood High 30068506
2011 ADAM8 metalloprotease is associated with PSGL-1 through ERM proteins and proteolytically cleaves PSGL-1; ADAM8 knockdown increases PSGL-1 surface expression and enhances leukocyte rolling on P-selectin, identifying ADAM8 as a sheddase regulating PSGL-1 function. Co-immunoprecipitation via ERM proteins, ADAM8 siRNA knockdown, PSGL-1 surface expression assay, leukocyte rolling assay on P-selectin European journal of immunology Medium 22229154
2012 The ERM-binding sequence (EBS) of the PSGL-1 cytoplasmic tail (key residues Arg-337 and Lys-338) is required for leukocyte tethering and rolling on L-, P-, and E-selectin and for ERK activation; however, EBS is dispensable for Syk phosphorylation and E-selectin-induced slow rolling. EBS deletion and alanine-substitution mutagenesis in 32D leukocytes, rolling assays on selectins, ERK and Syk phosphorylation assays The Journal of biological chemistry High 22311979
2012 PSGL-1 does not require dimerization or cytoskeletal anchorage to signal β2 integrin-dependent slow rolling; FRAP documented cytoskeletal restraint of WT PSGL-1, and actin depolymerization or cytoplasmic-tail mutation increasing lateral mobility did not impair slow rolling, whereas chemokine-triggered arrest remained cytoskeleton-dependent. FRAP, latrunculin B treatment, retroviral transduction of WT and mutant PSGL-1 into PSGL-1−/− macrophages, rolling/arrest assays, β2-hybrid domain swing-out assay The Journal of biological chemistry High 22511754
2008 Sorting nexin 20/SLIC-1 interacts with the PSGL-1 cytoplasmic domain and directs PSGL-1 to endosomes via its Phox homology domain; loss of the murine SLIC-1 homologue does not alter PSGL-1 signaling or neutrophil adhesion, indicating SLIC-1 is a sorting but not signaling regulator. Yeast two-hybrid screen, co-immunoprecipitation, colocalization in endosomes, murine SLIC-1 KO functional assays European journal of immunology Medium 18196517
2003 Peritoneal macrophages synthesize and constitutively express P-selectin on their plasma membrane; P-selectin is rapidly internalized to lysosomes. Macrophage-macrophage interactions in flow are mediated by P-selectin on one macrophage binding PSGL-1 on another. Metabolic labeling, flow cytometry, Western blot, immunoelectron microscopy, flow-based macrophage aggregation assay with blocking antibodies The Journal of cell biology Medium 14662752
2009 Flotillin-1 and -2 co-immunoprecipitate and colocalize with PSGL-1 in resting and stimulated neutrophils, associating with PSGL-1 in actin-dependent membrane microdomains that accumulate in the uropod; PSGL-1 is not required for formation of flotillin caps. Co-immunoprecipitation, immunofluorescence colocalization, PSGL-1−/− neutrophils, differentiated HL-60 overexpression PloS one Medium 19404397
2004 Sulfated tyrosines at the PSGL-1 N-terminus (not O-glycans or N-glycans) mediate interaction with the chemokine CCL27 (CTACK); mutation of N-terminal tyrosines to phenylalanine abolishes binding, and PSGL-1 expression on CCR10+ cells reduces chemotactic responses to CCL27. Recombinant PSGL-1-Ig binding assay with multiple chemokines, arylsulfatase/glycosidase treatment, sulfation-inhibitor synthesis, Tyr→Phe mutagenesis, CCR10+ cell chemotaxis assay The Journal of biological chemistry High 15466853
2015 The N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19; chemical-shift mapping shows the interactions are competitive, providing a structural basis for PSGL-1's enhancement of CCL19-mediated T cell recruitment. NMR solution structure of CCL19, chemical shift perturbation mapping with PSGL-1 and CCR7 N-terminal peptides Biochemistry Medium 26115234
2007 EphB4 activation with ephrin-B2-Fc upregulates PSGL-1 expression on endothelial progenitor cells; PSGL-1 siRNA reverses both the enhanced EPC adhesion to E/P-selectin and the proangiogenic effect of EphB4 activation in a mouse hindlimb ischemia model. EphB4 siRNA, PSGL-1 siRNA, EPC adhesion assay, mouse hindlimb ischemia model, neutralizing antibodies to E/P-selectin The Journal of clinical investigation Medium 17510705
2013 A single amino acid at EV71 capsid VP1-145 acts as a molecular switch controlling binding to PSGL-1: G or Q at VP1-145 permits binding to PSGL-1 sulfated tyrosines (mediated by conserved lysines VP1-242/244), while E at VP1-145 turns the VP1-244 lysine inward to prevent PSGL-1 binding. Site-directed mutagenesis of VP1-145, VP1-242, and VP1-244; cell binding assays on PSGL-1-expressing cells; crystal structure comparison of EV71 isolates PLoS pathogens High 23935488
2013 EV71 entry mediated by PSGL-1 requires caveolar endocytosis (caveolin-1-dependent), whereas SCARB2-mediated entry uses clathrin-dependent endocytosis; PSGL-1 and SCARB2 thus dictate distinct endocytic routes for EV71. Specific endocytosis inhibitors, caveolin-1 siRNA, clathrin knockdown, confocal colocalization of EV71 with caveolae in Jurkat T cells and PSGL-1-L929 cells vs. RD cells Journal of virology High 23760234
2016 Soluble Siglec-5 binds PSGL-1 on leukocytes in a sialic-acid-dependent and calcium-dependent manner (sialidase treatment reduces binding by 79%); PSGL-1 and Siglec-5 are in close proximity (<40 nm) on PBMCs. Soluble Siglec-5 variants block PSGL-1-mediated leukocyte rolling on E/P-selectin in vitro and in vivo. Soluble Siglec-5 binding assay, sialidase treatment, Duolink proximity ligation assay, in vitro perfusion rolling assay, in vivo TNF-α inflammation model Scientific reports Medium 27892504
2021 GALNT4-catalyzed O-glycosylation of PSGL-1 is required for P-selectin-induced β2 integrin activation on monocytes and for monocyte adhesion and transmigration; GALNT4 overexpression increases PSGL-1 O-glycosylation and activates Akt/mTOR and IκBα/NFκB downstream of P-selectin/PSGL-1 engagement. VVL lectin pulldown, PSGL-1 immunoprecipitation for O-glycosylation, GALNT4 shRNA knockdown and overexpression, β2-integrin activation assay, monocyte adhesion/transmigration under flow, ApoE−/− mouse atherosclerosis model Journal of molecular and cellular cardiology Medium 34974060
2023 P-selectin binding to PSGL-1 on tumor-associated macrophages activates the JNK/STAT1 pathway, driving transcription of complement C5 and release of C5a, which activates C5aR1 to shift TAMs toward a pro-tumor phenotype; PSGL-1 inhibition reduces CRC growth. Western blot for JNK/STAT1, dual-luciferase reporter and ChIP assay for C5 transcription, ELISA for C5a, siRNA knockdown of PSGL-1, AOM/DSS mouse CRC model, FACS Theranostics Medium 37064877
2023 P-selectin stimulation of neutrophils via PSGL-1 activates the Syk/Ca2+/PAD4 signaling pathway to induce NET formation; pharmacological inhibition of P-selectin (PSI-697) reduces PAD4 expression and NETs in pancreatic tissue and ameliorates acute pancreatitis in mice. Western blot for Syk phosphorylation, intracellular calcium imaging, PAD4 expression assay, flow cytometry and immunofluorescence for NETs, PSI-697 treatment in caerulein-AP mouse model Frontiers in immunology Medium 37841279
2014 PSGL-1 (CD162) but not CD44, when deleted by CRISPR-Cas9 from AML cell line KG1a, abolishes E-selectin-mediated chemo-resistance in vitro; absence of CD162 on AML cells delays leukemia onset, reduces BM retention, and increases chemotherapy sensitivity in vivo. CRISPR-Cas9 knockout of CD162 or CD44, in vitro E-selectin adhesion/chemo-resistance assays, preclinical murine AML model Frontiers in cell and developmental biology High 32793603
2014 Basic residues in HIV-1 Gag matrix domain and a polybasic sequence in the PSGL-1 cytoplasmic tail mediate coclustering of PSGL-1 with assembling Gag at the plasma membrane, promoting virion incorporation of PSGL-1. Quantitative two-color superresolution localization microscopy, Gag matrix mutants, PSGL-1 cytoplasmic tail chimera experiments in T and HeLa cells Journal of virology Medium 25320329
2020 PSGL-1 expression in virus-producing cells impairs incorporation of SARS-CoV and SARS-CoV-2 spike glycoproteins into pseudovirions and blocks pseudovirus attachment and infection of target cells, extending PSGL-1's antiviral restriction to coronaviruses. Pseudovirus infectivity assays, spike glycoprotein incorporation Western blot, PSGL-1 expression in virus-producing cells Viruses Medium 33396594
2005 PSGL-1 cross-linking induces tyrosine-phosphorylation-dependent and c-Abl-involved F-actin polymerization and redistribution in neutrophils; c-Abl relocalizes to F-actin foci, and the Abl inhibitor STI571 blocks the cytoskeletal reorganization. Anti-PSGL-1 antibody cross-linking, cytoskeletal fractionation, genistein and STI571 inhibition, F-actin immunofluorescence and redistribution assay Journal of cellular biochemistry Medium 15526280
1998 Characterization of O-linked oligosaccharides on PSGL-1 isolated from HL-60 cells showed that only ~4.5% of O-linked glycans (core-2 structures with sialic acid and fucose on N-acetyllactosamine) constitute the selectin-binding fraction; most O-glycans lack fucose and are insufficient for selectin binding. 3H-glucosamine metabolic labeling, P-selectin/E-selectin affinity chromatography, ion-exchange/size exclusion/lectin/paper chromatography, exoglycosidase treatments, chemical modifications Glycoconjugate journal Medium 10211703
2014 PI3K is required for PSGL-1-induced β1 integrin clustering (but not for β1 integrin conformation changes or total expression), and PSGL-1-PI3K-β1 integrin signaling promotes Jurkat cell adhesion to fibronectin. PI3K inhibitors, anti-PSGL-1 antibody ligation, β1 integrin clustering assay, conformation-sensitive antibody assay, Jurkat adhesion to fibronectin Molecular and cellular biochemistry Low 24122451

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Insights into the molecular basis of leukocyte tethering and rolling revealed by structures of P- and E-selectin bound to SLe(X) and PSGL-1. Cell 603 11081633
1995 PSGL-1 recognition of P-selectin is controlled by a tyrosine sulfation consensus at the PSGL-1 amino terminus. Cell 346 7585950
2000 P-Selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions in vivo. The Journal of experimental medicine 336 10770806
2019 VISTA is an acidic pH-selective ligand for PSGL-1. Nature 317 31645726
1999 Platelet/polymorphonuclear leukocyte interaction: P-selectin triggers protein-tyrosine phosphorylation-dependent CD11b/CD18 adhesion: role of PSGL-1 as a signaling molecule. Blood 280 9920836
1999 Targeted gene disruption demonstrates that P-selectin glycoprotein ligand 1 (PSGL-1) is required for P-selectin-mediated but not E-selectin-mediated neutrophil rolling and migration. The Journal of experimental medicine 250 10601352
2007 Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44. Immunity 238 17442598
1997 P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migration into inflamed skin. The Journal of experimental medicine 195 9053457
2004 Role of P-selectin and PSGL-1 in coagulation and thrombosis. Thrombosis and haemostasis 187 15351841
2005 Recruitment of adult thymic progenitors is regulated by P-selectin and its ligand PSGL-1. Nature immunology 182 15880112
2002 ITAM-based interaction of ERM proteins with Syk mediates signaling by the leukocyte adhesion receptor PSGL-1. Immunity 173 12387735
2010 E-selectin engages PSGL-1 and CD44 through a common signaling pathway to induce integrin alphaLbeta2-mediated slow leukocyte rolling. Blood 169 20299514
2008 PSGL-1 engagement by E-selectin signals through Src kinase Fgr and ITAM adapters DAP12 and FcR gamma to induce slow leukocyte rolling. The Journal of experimental medicine 168 18794338
2009 PSGL-1 function in immunity and steady state homeostasis. Immunological reviews 143 19594630
2007 Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling. Blood 142 17132726
1998 A novel P-selectin glycoprotein ligand-1 monoclonal antibody recognizes an epitope within the tyrosine sulfate motif of human PSGL-1 and blocks recognition of both P- and L-selectin. Blood 142 9414280
2003 PSGL-1 participates in E-selectin-mediated progenitor homing to bone marrow: evidence for cooperation between E-selectin ligands and alpha4 integrin. Blood 140 12763924
1996 P-selectin glycoprotein ligand-1 (PSGL-1) is a ligand for L-selectin in neutrophil aggregation. Blood 139 8839831
2016 PSGL-1 Is an Immune Checkpoint Regulator that Promotes T Cell Exhaustion. Immunity 132 27192578
2012 PSGL-1/selectin and ICAM-1/CD18 interactions are involved in macrophage-induced drug resistance in myeloma. Leukemia 131 22996336
2014 VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells. The Journal of experimental medicine 128 25135296
2017 PSGL-1: A New Player in the Immune Checkpoint Landscape. Trends in immunology 121 28262471
2002 Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters. Biophysical journal 111 11916843
2007 PSGL-1-mediated activation of EphB4 increases the proangiogenic potential of endothelial progenitor cells. The Journal of clinical investigation 107 17510705
2000 Activation of human leukocytes reduces surface P-selectin glycoprotein ligand-1 (PSGL-1, CD162) and adhesion to P-selectin in vitro. Journal of immunology (Baltimore, Md. : 1950) 105 10946308
2013 Enterovirus 71 binding to PSGL-1 on leukocytes: VP1-145 acts as a molecular switch to control receptor interaction. PLoS pathogens 103 23935488
2013 The PSGL-1-L-selectin signaling complex regulates neutrophil adhesion under flow. The Journal of experimental medicine 94 24127491
1999 PSGL-1-mediated adhesion of human hematopoietic progenitors to P-selectin results in suppression of hematopoiesis. Immunity 92 10514015
2018 Cooperative PSGL-1 and CXCR2 signaling in neutrophils promotes deep vein thrombosis in mice. Blood 91 30068506
2008 Separable requirements for cytoplasmic domain of PSGL-1 in leukocyte rolling and signaling under flow. Blood 89 18550846
2017 The Interaction of Selectins and PSGL-1 as a Key Component in Thrombus Formation and Cancer Progression. BioMed research international 84 28680883
2000 Sialyl Lewis(x)-mediated, PSGL-1-independent rolling adhesion on P-selectin. Biophysical journal 77 10920004
2002 Attachment of the PSGL-1 cytoplasmic domain to the actin cytoskeleton is essential for leukocyte rolling on P-selectin. Blood 75 12036880
2009 PSGL-1-dependent myeloid leukocyte activation. Journal of leukocyte biology 73 19703898
2007 PSGL-1 regulates platelet P-selectin-mediated endothelial activation and shedding of P-selectin from activated platelets. Thrombosis and haemostasis 68 17938805
2004 Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII. The Journal of biological chemistry 64 15579466
2019 Proteomic profiling of HIV-1 infection of human CD4+ T cells identifies PSGL-1 as an HIV restriction factor. Nature microbiology 63 30833724
1999 Structure and function of the selectin ligand PSGL-1. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 62 10412562
2007 PSGL-1 and mTOR regulate translation of ROCK-1 and physiological functions of macrophages. The EMBO journal 58 17245434
2002 Molecular basis of leukocyte rolling on PSGL-1. Predominant role of core-2 O-glycans and of tyrosine sulfate residue 51. The Journal of biological chemistry 58 12403782
2009 Fluorinated per-acetylated GalNAc metabolically alters glycan structures on leukocyte PSGL-1 and reduces cell binding to selectins. Blood 57 19996411
2000 A hematopoietic cell L-selectin ligand that is distinct from PSGL-1 and displays N-glycan-dependent binding activity. Blood 55 11023510
2020 PSGL-1 restricts HIV-1 infectivity by blocking virus particle attachment to target cells. Proceedings of the National Academy of Sciences of the United States of America 54 32273392
2010 Primary effusion lymphoma: genomic profiling revealed amplification of SELPLG and CORO1C encoding for proteins important for cell migration. The Journal of pathology 52 20690162
2009 Flotillins interact with PSGL-1 in neutrophils and, upon stimulation, rapidly organize into membrane domains subsequently accumulating in the uropod. PloS one 51 19404397
2013 Caveolar endocytosis is required for human PSGL-1-mediated enterovirus 71 infection. Journal of virology 50 23760234
2009 Peripheral blood CD4+ T lymphocytes from multiple sclerosis patients are characterized by higher PSGL-1 expression and transmigration capacity across a human blood-brain barrier-derived endothelial cell line. Journal of leukocyte biology 49 19696154
2001 differential L-selectin binding activities of human hematopoietic cell L-selectin ligands, HCELL and PSGL-1. The Journal of biological chemistry 49 11591704
2019 The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of dendritic cells by the TLR4 signaling pathway. Cell death & disease 48 31263109
1998 Dimerization of P-selectin glycoprotein ligand-1 (PSGL-1) required for optimal recognition of P-selectin. The Journal of cell biology 48 9660879
2004 Immunoblockade of PSGL-1 attenuates established experimental murine colitis by reduction of leukocyte rolling. American journal of physiology. Gastrointestinal and liver physiology 46 15001428
2021 PSGL-1 Immune Checkpoint Inhibition for CD4+ T Cell Cancer Immunotherapy. Frontiers in immunology 45 33708224
2008 Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status. Arteriosclerosis, thrombosis, and vascular biology 45 18497306
2002 Human mast cell progenitors use alpha4-integrin, VCAM-1, and PSGL-1 E-selectin for adhesive interactions with human vascular endothelium under flow conditions. Blood 43 11929779
2021 A PSGL-1 glycomimetic reduces thrombus burden without affecting hemostasis. Blood 42 33945603
2014 PSGL-1 and E/P-selectins are essential for T-cell rolling in inflamed CNS microvessels but dispensable for initiation of EAE. European journal of immunology 42 24740164
2014 Distribution of EV71 receptors SCARB2 and PSGL-1 in human tissues. Virus research 40 24997419
2007 Impact of carrier stiffness and microtopology on two-dimensional kinetics of P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) interactions. The Journal of biological chemistry 40 17267403
2001 Neutrophils, monocytes, and dendritic cells express the same specialized form of PSGL-1 as do skin-homing memory T cells: cutaneous lymphocyte antigen. Biochemical and biophysical research communications 40 11453631
2023 Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages. Theranostics 39 37064877
2007 P-selectin cross-links PSGL-1 and enhances neutrophil adhesion to fibrinogen and ICAM-1 in a Src kinase-dependent, but GPCR-independent mechanism. Cell adhesion & migration 39 19262138
2004 Blockade of PSGL-1 attenuates CD14+ monocytic cell recruitment in intestinal mucosa and ameliorates ileitis in SAMP1/Yit mice. Journal of leukocyte biology 39 15569697
2003 SELPLG gene polymorphisms in relation to plasma SELPLG levels and coronary artery disease. Annals of human genetics 39 14641238
2020 Acute Myeloid Leukemia Chemo-Resistance Is Mediated by E-selectin Receptor CD162 in Bone Marrow Niches. Frontiers in cell and developmental biology 38 32793603
2003 Peritoneal macrophages express both P-selectin and PSGL-1. The Journal of cell biology 37 14662752
2005 Signaling function of PSGL-1 in neutrophil: tyrosine-phosphorylation-dependent and c-Abl-involved alteration in the F-actin-based cytoskeleton. Journal of cellular biochemistry 36 15526280
2007 Structural basis of PSGL-1 binding to ERM proteins. Genes to cells : devoted to molecular & cellular mechanisms 34 18076570
2004 Polymorphisms in the P-selectin (CD62P) and P-selectin glycoprotein ligand-1 (PSGL-1) genes and coronary heart disease. Clinical chemistry and laboratory medicine 34 15497463
2015 Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites. Biochemistry 33 26115234
2012 Ezrin-radixin-moesin-binding sequence of PSGL-1 glycoprotein regulates leukocyte rolling on selectins and activation of extracellular signal-regulated kinases. The Journal of biological chemistry 33 22311979
2012 Signal-dependent slow leukocyte rolling does not require cytoskeletal anchorage of P-selectin glycoprotein ligand-1 (PSGL-1) or integrin αLβ2. The Journal of biological chemistry 33 22511754
2023 PSGL-1 attenuates early TCR signaling to suppress CD8+ T cell progenitor differentiation and elicit terminal CD8+ T cell exhaustion. Cell reports 32 37115668
2020 Virion-incorporated PSGL-1 and CD43 inhibit both cell-free infection and transinfection of HIV-1 by preventing virus-cell binding. Proceedings of the National Academy of Sciences of the United States of America 32 32193343
2016 Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity. Scientific reports 32 27892504
2011 The metalloprotease ADAM8 is associated with and regulates the function of the adhesion receptor PSGL-1 through ERM proteins. European journal of immunology 32 22229154
2003 Exogenous eosinophil activation converts PSGL-1-dependent binding to CD18-dependent stable adhesion to platelets in shear flow. American journal of physiology. Cell physiology 32 12529243
2015 Inhibition of P-Selectin and PSGL-1 Using Humanized Monoclonal Antibodies Increases the Sensitivity of Multiple Myeloma Cells to Bortezomib. BioMed research international 31 26539491
2013 Metastatic growth progression caused by PSGL-1-mediated recruitment of monocytes to metastatic sites. Cancer research 30 24322980
2004 Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. The Journal of biological chemistry 30 15466853
2014 Basic motifs target PSGL-1, CD43, and CD44 to plasma membrane sites where HIV-1 assembles. Journal of virology 29 25320329
2016 PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease. PLoS pathogens 28 26975045
2022 Targeting the PSGL-1 Immune Checkpoint Promotes Immunity to PD-1-Resistant Melanoma. Cancer immunology research 27 35303066
2019 Acute Myeloid and Lymphoblastic Leukemia Cell Interactions with Endothelial Selectins: Critical Role of PSGL-1, CD44 and CD43. Cancers 26 31461905
2017 Resveratrol attenuates hydrogen peroxide‑induced apoptosis, reactive oxygen species generation, and PSGL‑1 and VWF activation in human umbilical vein endothelial cells, potentially via MAPK signalling pathways. Molecular medicine reports 26 29207192
2008 Surprising up-regulation of P-selectin glycoprotein ligand-1 (PSGL-1) in endotoxin-induced uveitis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26 19050046
2007 T cell-associated CD18 but not CD62L, ICAM-1, or PSGL-1 is required for the induction of chronic colitis. American journal of physiology. Gastrointestinal and liver physiology 26 17332469
2005 P-selectin glycoprotein ligand-1 (PSGL-1) is up-regulated on leucocytes from patients with chronic obstructive pulmonary disease. Clinical and experimental immunology 26 16232226
2004 Endotoxin down-modulates P-selectin glycoprotein ligand-1 (PSGL-1, CD162) on neutrophils in humans. Journal of clinical immunology 25 14997035
2012 PSGL-1 regulates the migration and proliferation of CD8(+) T cells under homeostatic conditions. Journal of immunology (Baltimore, Md. : 1950) 24 22250093
2020 PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity. Viruses 23 33396594
1998 Characterization of the O-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1). Glycoconjugate journal 23 10211703
2022 PD-1 Immune Checkpoint Blockade and PSGL-1 Inhibition Synergize to Reinvigorate Exhausted T Cells. Frontiers in immunology 22 35774790
2008 SLIC-1/sorting nexin 20: a novel sorting nexin that directs subcellular distribution of PSGL-1. European journal of immunology 22 18196517
2005 Biomechanics of P-selectin PSGL-1 bonds: shear threshold and integrin-independent cell adhesion. Biophysical journal 22 16387772
2021 High expression of neutrophil and monocyte CD64 with simultaneous lack of upregulation of adhesion receptors CD11b, CD162, CD15, CD65 on neutrophils in severe COVID-19. Therapeutic advances in infectious disease 21 34377464
2020 PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteins. Cell discovery 20 32802403
2014 PI3K is involved in β1 integrin clustering by PSGL-1 and promotes β1 integrin-mediated Jurkat cell adhesion to fibronectin. Molecular and cellular biochemistry 20 24122451
2004 A down-regulatable E-selectin ligand is functionally important for PSGL-1-independent leukocyte-endothelial cell interactions. Blood 20 15304396
2023 High expression of P-selectin induces neutrophil extracellular traps via the PSGL-1/Syk/Ca2+/PAD4 pathway to exacerbate acute pancreatitis. Frontiers in immunology 19 37841279
2021 GALNT4 primes monocytes adhesion and transmigration by regulating O-Glycosylation of PSGL-1 in atherosclerosis. Journal of molecular and cellular cardiology 19 34974060

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