Affinage

SELPLG

P-selectin glycoprotein ligand 1 · UniProt Q14242

Length
412 aa
Mass
43.2 kDa
Annotated
2026-04-28
100 papers in source corpus 45 papers cited in narrative 45 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PSGL-1 (P-selectin glycoprotein ligand-1) is a dimeric sialomucin on leukocytes and platelets that serves as the primary counter-receptor for P-, E-, and L-selectins, mediating leukocyte tethering, rolling, and recruitment to activated endothelium, and additionally functions as a signaling receptor that regulates integrin activation, T cell exhaustion, chemokine responsiveness, and antiviral restriction. Selectin recognition requires cooperative engagement of tyrosine-sulfated residues (Tyr-48 for P-selectin, Tyr-51 for L-selectin) and core-2 sialyl-Lewis X O-glycans on Thr-57, with dimerization through Cys-320 essential for P-selectin binding, and fucosyltransferase VII-dependent glycosylation conferring E-selectin specificity (PMID:7585950, PMID:9660879, PMID:12403782, PMID:9353122). The cytoplasmic tail anchors PSGL-1 to the actin cytoskeleton via ERM proteins (moesin/radixin), and upon selectin engagement signals through Src family kinases (Fgr, Hck, Lyn), ITAM adaptors (DAP12, FcRγ), and Syk to activate β2-integrins for slow rolling and neutrophil arrest, while also modulating T cell exhaustion by attenuating TCR-Zap70 signaling and upregulating PD-1 via interaction with VISTA at acidic pH (PMID:18794338, PMID:12036880, PMID:27192578, PMID:31645726, PMID:37115668). PSGL-1 also acts as an interferon-induced antiviral restriction factor that blocks HIV-1 virion attachment through its ectodomain, sequesters gp41 to inhibit Env incorporation, and is counteracted by Vpu-mediated ubiquitination and degradation via SCFβ-TrCP2 (PMID:30833724, PMID:32802403, PMID:32273392).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1995 High

    Identifying that N-terminal tyrosine sulfation is required for P-selectin binding established the first post-translational modification critical for PSGL-1 adhesive function, moving beyond simple glycoprotein recognition.

    Evidence Site-directed mutagenesis of N-terminal tyrosines and sulfation inhibitor treatment with rolling adhesion assays

    PMID:7585950

    Open questions at the time
    • Relative contributions of individual tyrosine residues not yet resolved
    • Structural basis of sulfated tyrosine–P-selectin interaction unknown at this point
  2. 1997 High

    Demonstrating that CLA is an inducible fucosyltransferase VII-dependent carbohydrate modification of PSGL-1 that selectively confers E-selectin binding revealed that the same protein backbone can be differentially glycosylated to regulate distinct selectin interactions.

    Evidence T cell differentiation assays, monoclonal antibody characterization, and selectin-binding functional assays

    PMID:9353122

    Open questions at the time
    • Glycosyltransferase regulation during T cell differentiation not mechanistically defined
    • Whether other fucosyltransferases contribute was unresolved
  3. 1998 High

    Showing that Cys-320-mediated disulfide-bonded dimerization is essential for P-selectin rolling established PSGL-1's quaternary structure as a functional requirement, not merely a structural feature.

    Evidence C320A mutagenesis, Western blotting under native conditions, and flow-based rolling assays

    PMID:9660879

    Open questions at the time
    • Whether dimerization is equally required for E- and L-selectin binding was untested
  4. 1999 High

    Demonstrating that P-selectin engagement of PSGL-1 triggers tyrosine kinase signaling leading to β2-integrin activation transformed PSGL-1 from a passive adhesion molecule into a signaling receptor that couples rolling to firm adhesion.

    Evidence Mixed cell aggregation under shear, tyrosine kinase inhibitors, and P-selectin-IgG stimulation of PMN

    PMID:9920836

    Open questions at the time
    • Identity of the specific kinases downstream of PSGL-1 not yet mapped
    • Role of the cytoplasmic domain in signaling not defined
  5. 2002 High

    Defining that the PSGL-1 cytoplasmic tail links to actin via moesin, and that this moesin–Syk axis transduces ITAM-dependent signaling, provided the first molecular bridge between PSGL-1 adhesion and intracellular kinase activation.

    Evidence Co-immunoprecipitation with ERM proteins, cytoplasmic truncation mutants, Syk inhibitors, ITAM-mutated moesin, and SRE reporter assays

    PMID:12036880 PMID:12387735

    Open questions at the time
    • How moesin ITAM-like motifs recruit Syk in the absence of classical ITAM adaptors was unclear
    • Structural details of the PSGL-1–moesin interface not yet resolved
  6. 2002 High

    Systematic mutagenesis of Thr-57, Tyr-48, and Tyr-51 defined the differential post-translational requirements for L-selectin versus P-selectin binding, establishing that the same N-terminal region uses distinct residues for each selectin.

    Evidence Site-directed mutagenesis with L-selectin binding assays and leukocyte rolling on CHO transfectants co-expressing glycosyltransferases

    PMID:12403782

    Open questions at the time
    • Whether these requirements hold in vivo under inflammatory conditions was not tested
  7. 2004 High

    Demonstrating that PSGL-1 binds chemokine CCL27 via sulfated tyrosines and attenuates CCL27-mediated chemotaxis uncovered a selectin-independent function as a chemokine-interacting molecule.

    Evidence Tyrosine-to-phenylalanine mutagenesis, arylsulfatase and glycosidase treatment, and chemotaxis assays

    PMID:15466853

    Open questions at the time
    • Physiological significance of CCL27 sequestration in skin homing not confirmed in vivo
  8. 2005 High

    PSGL-1 on lymphoid progenitors was shown to mediate thymic homing via P-selectin on thymic endothelium, extending its role from inflammatory recruitment to developmental lymphocyte trafficking.

    Evidence Parabiosis, competitive thymus reconstitution, short-term homing assays in PSGL-1 knockout mice

    PMID:15880112

    Open questions at the time
    • Whether PSGL-1 glycosylation is regulated during thymic progenitor development was not explored
  9. 2007 High

    Multiple discoveries in 2007 consolidated a picture of PSGL-1 as both a dominant capture receptor for E-selectin on neutrophils and a facilitator of homeostatic T cell homing through CCL19/CCL21 binding, independent of selectins, while also signaling through Akt/mTOR to regulate ROCK-1 translation in macrophages.

    Evidence Knockout and siRNA epistasis with intravital microscopy for E-selectin ligand hierarchy; PSGL-1-null T cell chemotaxis assays; polysome profiling and rapamycin inhibition in PSGL-1 KO macrophages

    PMID:17245434 PMID:17401367 PMID:17442598

    Open questions at the time
    • Structural basis of PSGL-1–CCL19/CCL21 interaction unknown
    • How PSGL-1 selectively regulates ROCK-1 but not ROCK-2 mRNA translation not mechanistically explained
  10. 2007 High

    Crystal structure of the radixin FERM domain with the PSGL-1 juxtamembrane peptide revealed β-strand-mediated binding in the subdomain C groove, providing the first atomic-level view of how PSGL-1 connects to the ERM–actin cytoskeleton.

    Evidence X-ray crystallography of radixin FERM domain–PSGL-1 peptide complex

    PMID:18076570

    Open questions at the time
    • Whether the radixin and moesin FERM domains engage PSGL-1 identically was not compared structurally
  11. 2008 High

    Defining the Fgr/Hck/Lyn → DAP12/FcRγ → Syk signaling cascade downstream of E-selectin–PSGL-1 engagement, and showing the cytoplasmic domain is essential for integrin activation but dispensable for rolling, separated the adhesive and signaling functions of PSGL-1 at the genetic level.

    Evidence Multiple compound-knockout mice (Fgr, Hck/Lyn/Fgr, DAP12/FcRγ), ΔCD knock-in mice, intravital microscopy, and phosphorylation assays

    PMID:18550846 PMID:18794338

    Open questions at the time
    • How PSGL-1 cytoplasmic domain recruits SFKs to lipid rafts was mechanistically undefined
    • Whether DAP12/FcRγ directly bind PSGL-1 or are recruited indirectly was unresolved
  12. 2010 High

    Identifying that EV71 binds PSGL-1 via sulfated tyrosines (not O-glycans) established PSGL-1 as a viral entry receptor exploiting the same N-terminal motif used by selectins, revealing pathogen hijacking of an adhesion molecule.

    Evidence Systematic mutagenesis (T57A, sulfation inhibitors) with flow cytometry binding and viral replication assays in 293T and Jurkat cells

    PMID:21079683

    Open questions at the time
    • Whether PSGL-1-mediated EV71 entry occurs in vivo not demonstrated
    • Structural basis of EV71–PSGL-1 interaction not yet solved
  13. 2012 High

    Fine-mapping of the ERM-binding sequence (Arg-337/Lys-338) showed it is required for tethering/rolling and ERK activation but dispensable for Syk phosphorylation and slow rolling, demonstrating bifurcation of PSGL-1 cytoplasmic signaling into ERM-dependent and ERM-independent arms.

    Evidence Alanine substitution mutagenesis at R337/K338, rolling assays on all three selectins, and parallel measurement of ERK and Syk phosphorylation

    PMID:22311979

    Open questions at the time
    • How PSGL-1 activates Syk independently of ERM linkage was not explained
  14. 2013 High

    Discovery of a constitutive PSGL-1–L-selectin signaling complex on neutrophils that signals through SFKs and ITAM adaptors to activate LFA-1 revealed a cis-acting receptor complex, distinct from trans selectin–ligand interactions during rolling.

    Evidence Co-immunoprecipitation, L-selectin KO mice, flow chamber and intravital microscopy

    PMID:24127491

    Open questions at the time
    • Stoichiometry and structural basis of the cis PSGL-1–L-selectin complex unresolved
    • Whether this complex forms in non-neutrophil leukocytes was not tested
  15. 2016 High

    Showing that PSGL-1 ligation promotes CD8+ T cell exhaustion by upregulating PD-1 and inhibiting TCR/IL-2 signaling—with PSGL-1-deficient mice clearing chronic LCMV—recast PSGL-1 as an immune checkpoint molecule beyond its adhesion function.

    Evidence PSGL-1-deficient mice in chronic LCMV infection model, T cell function and receptor expression analysis

    PMID:27192578

    Open questions at the time
    • Ligand identity for PSGL-1 in the T cell exhaustion context was not established in this study
    • Mechanism linking PSGL-1 to PD-1 transcription not defined
  16. 2019 High

    Two major advances established PSGL-1 as both a pH-sensitive receptor for VISTA in the tumor microenvironment and an IFN-γ-induced antiviral restriction factor degraded by HIV-1 Vpu via SCFβ-TrCP2-mediated ubiquitination.

    Evidence Histidine mutagenesis and pH-selective binding/blocking antibodies with in vivo tumor models for VISTA; quantitative mass spectrometry, Co-IP, ubiquitination, and infectivity assays in primary CD4+ T cells for HIV-1

    PMID:30833724 PMID:31645726

    Open questions at the time
    • Structural basis of the pH-dependent VISTA–PSGL-1 interaction not solved
    • Whether PSGL-1 antiviral activity extends to other retroviruses was unexplored
  17. 2020 High

    Detailed mechanistic dissection showed PSGL-1 restricts HIV-1 through dual mechanisms—blocking virion attachment via its ectodomain and sequestering gp41 to prevent Env incorporation—while also binding F-actin directly to restrict actin dynamics needed for HIV DNA synthesis.

    Evidence Domain mapping, pseudovirus attachment assays, cryo-EM, super-resolution imaging, F-actin binding assays, Env incorporation assays

    PMID:32193343 PMID:32273392 PMID:32802403

    Open questions at the time
    • Whether F-actin binding by PSGL-1 is relevant to its canonical leukocyte functions was not tested
    • In vivo relevance of PSGL-1 antiviral restriction not demonstrated in animal models
  18. 2023 High

    PSGL-1 was positioned upstream of PD-1 in the exhaustion hierarchy: TCR co-ligation with PSGL-1 attenuates Zap70 phosphorylation and maintains the Zap70 inhibitor Sts-1, driving terminal exhaustion, which provided a mechanistic basis for PSGL-1's checkpoint function.

    Evidence PSGL-1-deficient mice in chronic infection and tumor models, Zap70 phosphorylation and Sts-1 expression assays, metabolic gene profiling

    PMID:37115668

    Open questions at the time
    • Direct physical interaction between PSGL-1 and TCR signaling components not demonstrated
    • Whether PSGL-1-mediated exhaustion involves VISTA as the ligand in these models is not established

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: (1) the structural basis of pH-dependent VISTA–PSGL-1 binding; (2) how PSGL-1 cytoplasmic domain recruits ITAM adaptors and Syk independently of ERM linkage; (3) in vivo validation of PSGL-1 antiviral restriction in animal models; (4) therapeutic targeting of PSGL-1 as an immune checkpoint distinct from PD-1.
  • No full-length PSGL-1 structure available
  • Therapeutic antibody or small molecule targeting PSGL-1 checkpoint function not yet tested clinically
  • Integration of PSGL-1 adhesion, signaling, and checkpoint functions into a unified model remains incomplete

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060089 molecular transducer activity 6 GO:0098631 cell adhesion mediator activity 6 GO:0001618 virus receptor activity 4 GO:0008092 cytoskeletal protein binding 3
Localization
GO:0005886 plasma membrane 6 GO:0005856 cytoskeleton 4 GO:0005768 endosome 1
Pathway
R-HSA-168256 Immune System 8 R-HSA-162582 Signal Transduction 6 R-HSA-1500931 Cell-Cell communication 5 R-HSA-1643685 Disease 5 R-HSA-109582 Hemostasis 3
Partners
DAP12MSNSELESELLSELPSNX20SYKVISTA
Complex memberships
PSGL-1 homodimerPSGL-1–L-selectin cis signaling complex

Evidence

Reading pass · 45 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 A short segment near the amino terminus of PSGL-1 containing a tyrosine sulfation consensus is essential for P-selectin adhesion; mutation of N-terminal tyrosines to phenylalanine abolishes P-selectin binding, and sulfation inhibitors greatly reduce binding. Site-directed mutagenesis, sulfation inhibitor treatment, rolling adhesion assay on P-selectin-coated coverslips Cell High 7585950
1997 CLA (cutaneous lymphocyte antigen), the skin-homing receptor on T cells, is an inducible carbohydrate modification (mediated by fucosyltransferase VII) of PSGL-1 that confers E-selectin binding; PSGL-1 without CLA binds P-selectin but not E-selectin, demonstrating independent regulation of selectin-binding phenotypes. Monoclonal antibody characterization, T cell differentiation assays, selectin-binding functional assays Nature High 9353122
1998 Dimerization of PSGL-1 through a single conserved extracellular cysteine (C320) is essential for functional recognition of P-selectin; a C320A monomeric mutant fails to support rolling or P-selectin chimera binding. Site-directed mutagenesis, Western blotting under native/denaturing conditions, rolling adhesion assay under physiologic flow, P-selectin chimera binding The Journal of cell biology High 9660879
1996 PSGL-1 serves as a ligand for L-selectin on neutrophils, mediating neutrophil aggregation; blocking PSGL-1 with Fab fragments of PL1 antibody reduces neutrophil aggregate number and size, consistent with L-selectin–PSGL-1 counter-receptor interaction. Flow cytometry aggregation assay, blocking antibody (Fab fragments) experiments Blood Medium 8839831
1999 P-selectin binding to PSGL-1 triggers tyrosine kinase-dependent (Syk-like) signaling that activates CD11b/CD18 (beta2-integrin) on PMN; PSGL-1 engagement with a non-adhesion-blocking antibody was sufficient to trigger beta2-integrin-dependent aggregation and tyrosine phosphorylation. Mixed cell aggregation assays under shear, blocking antibodies, tyrosine kinase inhibitors (genistein), CHO-P transfectants, P-selectin-IgG fusion protein stimulation Blood High 9920836
2000 PSGL-1 is expressed on platelets (at 25–100-fold lower levels than leukocytes) and mediates platelet rolling in mesenteric venules in vivo; anti-PSGL-1 antibody significantly reduced platelet rolling as shown by intravital microscopy. P-selectin-IgG affinity purification, immunoelectron microscopy, Western blotting, flow cytometry, intravital microscopy The Journal of experimental medicine High 10770806
2000 The human granulocytic ehrlichiosis (HGE) bacterium infects neutrophils by specifically binding to fucosylated PSGL-1; blocking the P-selectin binding domain of PSGL-1 with monoclonal antibodies or enzymatic digestion prevents HGE cell binding and infection, and neoexpression of PSGL-1 with Fuc-TVII in non-susceptible cells confers susceptibility. Antibody blocking, enzymatic digestion, cDNA co-transfection of PSGL-1 + Fuc-TVII in non-susceptible cells Science High 10834846
2002 PSGL-1 associates with Syk via the actin-linking ERM proteins moesin and ezrin, which directly interact with Syk in an ITAM-dependent manner; PSGL-1 engagement induces Syk tyrosine phosphorylation and SRE-dependent transcriptional activity, establishing PSGL-1 as a signaling receptor. Co-immunoprecipitation, Syk inhibitor (piceatannol), dominant-negative Syk and ITAM-mutated moesin overexpression, transcriptional activity reporter assay Immunity High 12387735
2002 Attachment of the PSGL-1 cytoplasmic domain to the actin cytoskeleton via selective interaction with moesin (but not other ERM proteins) is essential for leukocyte rolling on P-selectin; truncation of the cytoplasmic domain abrogates rolling despite intact surface expression and P-selectin binding. Stable transfectants with truncated PSGL-1, rolling adhesion assays, actin cytoskeletal toxins, co-immunoprecipitation with ERM proteins Blood High 12036880
2002 Core-2 O-glycans on Thr-57 of PSGL-1 and tyrosine sulfation at Tyr-51 (predominant for L-selectin; Tyr-48 predominant for P-selectin) are critical structural determinants for L-selectin binding and leukocyte rolling on PSGL-1. Site-directed mutagenesis of Thr/Tyr residues, soluble L-selectin binding, leukocyte rolling assays on transfected CHO cells, co-expression of glycosyltransferases The Journal of biological chemistry High 12403782
2003 P-selectin interaction with PSGL-1 induces formation of procoagulant microparticles from human blood; PSGL-1-deficient mice produce fewer microparticles after P-selectin chimera infusion and fail to increase microparticle count with aging. P-selectin-Ig chimera infusion, PSGL-1 knockout mice, microparticle quantification, tail-bleeding time in hemophilia A mice Nature medicine High 12858167
2004 Human FucT-IV and FucT-VII both contribute to PSGL-1 selectin-binding activity, with FucT-VII playing the predominant role; core-2 O-glycans on Thr-57 are critical for L- and P-selectin rolling but additional binding sites support >75% of E-selectin-mediated rolling. CHO cell transfection with FucT-IV and/or FucT-VII, rolling adhesion assays, Thr-57 mutagenesis The Journal of biological chemistry High 15579466
2005 P-selectin expressed on thymic endothelium binds PSGL-1 on lymphoid progenitors to mediate thymic homing; PSGL-1-deficient mice have fewer early thymic progenitors and increased empty niches, and the number of resident thymic progenitors controls thymic P-selectin expression. Parabiosis, competitive thymus reconstitution, short-term homing assays, PSGL-1 knockout mice Nature immunology High 15880112
2007 PSGL-1, ESL-1, and CD44 together constitute all E-selectin ligand activity on neutrophils: PSGL-1 dominates initial capture, ESL-1 is critical for converting tethers to slow rolling, and CD44 controls rolling velocity and mediates E-selectin-dependent redistribution of PSGL-1 and L-selectin to the uropod pole via p38 signaling. Gene knockout and RNA interference loss-of-function in mice and cells, intravital microscopy, flow chamber assays Immunity High 17442598
2007 PSGL-1 mediates an enhanced chemotactic T cell response to homeostatic chemokines CCL21 and CCL19 (but not inflammatory chemokines), facilitating T cell homing to secondary lymphoid organs independently of selectin binding. PSGL-1-null mice, homing assays, T cell chemotaxis assays Nature immunology High 17401367
2007 EphB4 activation upregulates PSGL-1 expression on endothelial progenitor cells (EPCs) and enhances EPC adhesion to E- and P-selectin; PSGL-1 siRNA reverses the proangiogenic and adhesive effects of EphB4 activation, placing PSGL-1 downstream of EphB4 in EPC angiogenic signaling. siRNA knockdown of EphB4 and PSGL-1, EPC adhesion assays, neutralizing antibodies, mouse hind limb ischemia model The Journal of clinical investigation Medium 17510705
2007 PSGL-1 engagement in macrophages signals through Akt/mTOR to selectively upregulate translation of preformed ROCK-1 mRNA (not ROCK-2), enabling ROCK-1-dependent chemotaxis and phagocytosis; PSGL-1-deficient macrophages recapitulate effects of mTOR inhibition by rapamycin. PSGL-1 knockout mouse macrophages, rapamycin inhibition, polysome profiling, ROCK-1 protein detection, chemotaxis and phagocytosis assays The EMBO journal High 17245434
2007 Crystal structure of the radixin FERM domain in complex with the PSGL-1 juxtamembrane peptide reveals that the peptide binds the FERM subdomain C groove via a β-strand interaction; PSGL-1 lacks the canonical Motif-1 but compensates with non-conserved large residues (Met9, His8) that stabilize groove binding. X-ray crystallography of radixin FERM domain–PSGL-1 peptide complex Genes to cells : devoted to molecular & cellular mechanisms High 18076570
2008 E-selectin engagement of PSGL-1 signals through the Src family kinase Fgr and ITAM-containing adaptor proteins DAP12 and FcRγ to phosphorylate Syk, which is required for slow leukocyte rolling and neutrophil recruitment in vivo. Gene-deficient mice (fgr-/-, hck-/-lyn-/-fgr-/-, Tyrobp-/-Fcrg-/-), flow chamber assays, intravital microscopy, peritonitis model, Syk and p38 phosphorylation The Journal of experimental medicine High 18794338
2008 The PSGL-1 cytoplasmic domain is dispensable for neutrophil rolling on P-selectin but is essential for activating beta2-integrins (LFA-1) via Syk to induce slow rolling on ICAM-1; DeltaCD mice (cytoplasmic domain-deleted) roll normally on P-selectin but fail to trigger Syk-dependent LFA-1 activation. DeltaCD knock-in mice, flow chamber assays, OSGE digestion to match PSGL-1 density, Syk phosphorylation assay, intravital microscopy Blood High 18550846
2009 PSGL-1 interacts with homeostatic chemokines CCL19 and CCL21 to facilitate T-cell chemotaxis; O-glycan modifications that support selectin-mediated rolling interfere with PSGL-1 binding to these homeostatic chemokines, demonstrating that glycosylation reciprocally regulates inflammatory versus homeostatic trafficking functions. PSGL-1-null mice, chemotaxis assays, glycosyltransferase manipulation (review integrating primary data) Immunological reviews Medium 19594630
2010 E-selectin engagement of PSGL-1 or CD44 triggers slow leukocyte rolling through a common lipid raft-dependent pathway using Src kinases Hck, Lyn, and Fgr, Syk, and Bruton tyrosine kinase (Btk) as intermediates; the PSGL-1 cytoplasmic domain is required to activate SFKs and slow rolling but PI3K is not required. KO mice (Hck/Lyn/Fgr), Btk-deficient mice, lipid raft disruption, flow chamber and intravital microscopy, phosphorylation assays Blood High 20299514
2010 Tyrosine sulfation at the N-terminus of PSGL-1 (not O-glycosylation at T57) is critical for EV71 binding and infection of leukocytes; sodium chlorate (sulfation inhibitor) blocks PSGL-1–EV71 interaction and viral replication, while T57A mutation does not affect EV71 binding. PSGL-1 mutant expression in 293T cells, flow cytometry binding assay, sodium chlorate inhibition, viral replication assay in Jurkat cells PLoS pathogens High 21079683
2011 Tyrosine sulfation of mouse Psgl-1 is required for optimal P-selectin binding and leukocyte rolling in vivo; hematopoietic reconstitution with Tpst1;Tpst2 double-KO cells (lacking tyrosylprotein sulfotransferase activity) shows reduced P-selectin binding and increased rolling velocity despite equivalent PSGL-1 surface expression. Bone marrow transplantation with TPST double-KO donors, intravital microscopy, in vitro rolling assay on P-selectin, flow cytometry PloS one High 21633705
2012 The PSGL-1 ERM-binding sequence (EBS), specifically Arg-337 and Lys-338 in the cytoplasmic tail, is critical for leukocyte tethering and rolling on L-, P-, and E-selectin and for activating ERK; however, EBS is dispensable for Syk phosphorylation and E-selectin-induced slow rolling via LFA-1. Mutagenesis (EBS deletion, Arg/Lys alanine substitution), leukocyte rolling assays on selectin substrates, ERK and Syk phosphorylation assays The Journal of biological chemistry High 22311979
2013 A subset of PSGL-1 molecules is constitutively associated with L-selectin on neutrophils; this PSGL-1–L-selectin signaling complex signals through Src family kinases and ITAM adaptor proteins to activate LFA-1, with signaling output dependent on the L-selectin cytoplasmic tail. Co-immunoprecipitation, flow chamber assays, KO mice (L-selectin), neutrophil slow rolling and recruitment by intravital microscopy The Journal of experimental medicine High 24127491
2013 EV71 binds to the N-terminal region of PSGL-1 (sulfated tyrosines) via conserved lysine residues VP1-242 and VP1-244 on the capsid; VP1-145 acts as a molecular switch controlling PSGL-1 binding by modulating the orientation of VP1-244 lysine side chain. Site-directed mutagenesis (VP1-145, VP1-244, VP1-242), PSGL-1-expressing CHO cell binding assays, Jurkat T-cell infection, crystal structure comparison PLoS pathogens High 23935488
2013 PSGL-1-mediated EV71 entry proceeds via caveolar (caveolin-1-dependent) endocytosis requiring intact membrane cholesterol and acidification; this is distinct from hSCARB2-mediated entry which uses clathrin-dependent endocytosis. siRNA knockdown of caveolin-1, specific endocytosis inhibitors, confocal colocalization, pH inhibitors, PSGL-1-expressing L929 cells and Jurkat T cells Journal of virology High 23760234
2015 The N-termini of PSGL-1 and CCR7 have overlapping and competitive binding sites for CCL19, as determined by NMR chemical shift mapping; the solution structure of CCL19 reveals a canonical chemokine fold, and PSGL-1 competes with CCR7 for CCL19 binding. NMR solution structure of CCL19, chemical shift mapping, competitive binding assays Biochemistry High 26115234
2016 PSGL-1 ligation on exhausted CD8+ T cells inhibits TCR and IL-2 signaling and upregulates PD-1, leading to diminished survival upon TCR stimulation; PSGL-1-deficient mice clear chronic virus due to increased survival and multifunctionality of effector T cells with downregulated PD-1. PSGL-1-deficient mice, chronic viral infection model (LCMV), T cell function assays, receptor expression analysis Immunity High 27192578
2018 Cooperative PSGL-1 and CXCR2 signaling in rolling neutrophils promotes β2-integrin-dependent arrest and NETosis contributing to deep vein thrombosis; PSGL-1 signaling in DVT uses tyrosine 145 of SLP-76 rather than Y112/Y128, and does not require L-selectin in this context. Genetically engineered mice, ultrasonography, spinning-disk intravital microscopy, flow restriction DVT model, pharmacological blocking Blood High 30068506
2019 VISTA binds PSGL-1 selectively at acidic pH (as found in tumor microenvironments) via multiple histidine residues along the rim of the VISTA extracellular domain; this interaction suppresses T cells and antibodies that selectively block this interaction at acidic pH reverse VISTA-mediated immune suppression in vivo. Binding assays, pH titration experiments, histidine mutagenesis, pH-selective blocking antibodies, in vivo tumor models Nature High 31645726
2019 HIV-1 Vpu binds PSGL-1 and induces its ubiquitination and proteasomal degradation through the ubiquitin ligase SCFβ-TrCP2; PSGL-1 (induced by IFN-γ) inhibits HIV-1 reverse transcription and blocks virion infectivity by incorporating into progeny virions. Quantitative mass spectrometry proteomics, co-immunoprecipitation, ubiquitination assays, siRNA knockdown, viral infectivity assays in primary CD4+ T cells Nature microbiology High 30833724
2020 PSGL-1 incorporated into HIV-1 virions blocks virus particle attachment to target cells through its extracellular N-terminal domain; this inhibitory activity is glycoprotein-independent, and Vpu (primarily) and Nef downregulate PSGL-1 from the cell surface to partially escape restriction. PSGL-1 domain mapping, pseudovirus attachment assays, virus infectivity assays, flow cytometry, siRNA knockdown Proceedings of the National Academy of Sciences of the United States of America High 32273392
2020 PSGL-1 directly binds cellular F-actin to restrict actin dynamics, inhibiting HIV DNA synthesis; PSGL-1 also binds gp41 and sequesters it at the plasma membrane, blocking Env incorporation into nascent virions, causing loss of envelope spikes and profound defects in viral entry. F-actin binding assay, gp41 co-immunoprecipitation, cryo-electron microscopy, super-resolution imaging, HIV DNA synthesis assay, Env incorporation assay Cell discovery High 32802403
2020 Virion-incorporated PSGL-1 (and CD43) inhibits HIV-1 cell-free infection and transinfection by preventing virus-cell attachment; PSGL-1's full-length ectodomain is required for this inhibitory effect, and coclustering with HIV-1 Gag at uropod membranes depends on polybasic sequences in both the PSGL-1 cytoplasmic tail and Gag matrix domain. Virion incorporation assays, CD4+ and CD4- cell attachment assays, ectodomain truncation mutants, Gag colocalization studies Proceedings of the National Academy of Sciences of the United States of America High 32193343
2020 PSGL-1 impairs SARS-CoV and SARS-CoV-2 spike glycoprotein incorporation into pseudovirions and blocks pseudovirus attachment and infectivity of target cells. Pseudovirus infectivity assays, Western blotting for spike incorporation, cell attachment assays Viruses Medium 33396594
2021 GALNT4-mediated O-glycosylation of PSGL-1 promotes P-selectin-induced monocyte adhesion and transmigration by activating the Akt/mTOR and IκBα/NFκB pathways; GALNT4 knockdown reduces PSGL-1 O-glycosylation, attenuates Akt/mTOR and NFκB activation, and decreases monocyte adhesion to P-selectin. VVL pull-down, PSGL-1 immunoprecipitation, GALNT4 shRNA knockdown, GALNT4 overexpression, monocyte flow adhesion assay, mTOR inhibitor (rapamycin), phosphorylation assays Journal of molecular and cellular cardiology Medium 34974060
2023 PSGL-1 acts upstream of PD-1 and requires co-ligation with the TCR to attenuate early TCR signaling via Zap70 and maintain expression of the Zap70 inhibitor Sts-1, driving terminal CD8+ T cell exhaustion; PSGL-1 deficiency enables responses to low-affinity TCR ligands and sustains an elevated metabolic/glycolytic gene signature. PSGL-1-deficient mice, chronic infection/tumor models, TCR signaling assays (Zap70 phosphorylation), Sts-1 expression analysis, metabolic gene profiling Cell reports High 37115668
2008 Sorting nexin SLIC-1 (SNX20) directly and specifically interacts with the PSGL-1 cytoplasmic domain and contains a Phox homology domain that targets the PSGL-1/SLIC-1 complex to endosomes, cycling PSGL-1 into the endosomal compartment. Yeast two-hybrid screen, co-immunoprecipitation, colocalization experiments, motif mapping, SNX20-deficient mice European journal of immunology Medium 18196517
2004 Human PSGL-1 interacts with skin-associated chemokine CCL27 via sulfated tyrosines at its amino terminus (not glycans); PSGL-1 expression on CCR10-expressing cells reduces chemotaxis to CCL27, suggesting PSGL-1 regulates chemokine-mediated responses by sequestering CCL27. rPSGL-Ig binding assays, arylsulfatase and glycosidase treatments, sulfation inhibitor, tyrosine-to-phenylalanine mutagenesis, chemotaxis assays The Journal of biological chemistry High 15466853
2023 P-selectin (CD62P) binding to PSGL-1 on tumor-associated macrophages activates the JNK/STAT1 pathway to induce C5 transcription and C5a release, shifting TAMs toward a pro-tumor phenotype and promoting CRC growth via the C5a/C5aR1 axis. Western blotting, siRNA knockdown of PSGL-1, JNK/STAT1 pathway inhibitors, dual-luciferase reporter, ChIP assay, in vivo AOM/DSS CRC model Theranostics Medium 37064877
2005 PSGL-1 cross-linking in neutrophils triggers tyrosine-phosphorylation-dependent and c-Abl-involved alteration of F-actin cytoskeleton and PSGL-1 polarization; c-Abl redistributes to F-actin-concentrated regions upon PSGL-1 engagement. Anti-PSGL-1 antibody cross-linking, actin cytoskeleton staining, genistein (tyrosine kinase inhibitor), STI571 (c-Abl inhibitor), cytochalasin B treatment Journal of cellular biochemistry Medium 15526280
2009 Flotillin-1 and flotillin-2 associate with PSGL-1 in resting and stimulated neutrophils (shown by co-immunoprecipitation and colocalization) and reorganize into uropod domains; flotillin cap formation is PSGL-1-independent as shown by PSGL-1-deficient neutrophils, but PSGL-1 and flotillins redistribute together more rapidly than other uropod proteins. Co-immunoprecipitation, immunofluorescence, PSGL-1-deficient mice neutrophils, HL-60 differentiated cells PloS one Medium 19404397
2005 PSGL-1 engagement upregulates CSF-1 transcription in a Syk-dependent manner; overexpression of wild-type but not kinase-dead Syk promotes CSF-1 promoter activation downstream of PSGL-1, and Syk inhibitor piceatannol suppresses PSGL-1-induced CSF-1 mRNA upregulation. Antibody engagement of PSGL-1, CSF-1 promoter reporter assay, Syk dominant-negative overexpression, piceatannol inhibition, RT-PCR Cellular immunology Medium 16289055

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells. Nature 415 9353122
1995 PSGL-1 recognition of P-selectin is controlled by a tyrosine sulfation consensus at the PSGL-1 amino terminus. Cell 344 7585950
2000 P-Selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions in vivo. The Journal of experimental medicine 335 10770806
2019 VISTA is an acidic pH-selective ligand for PSGL-1. Nature 312 31645726
1999 Platelet/polymorphonuclear leukocyte interaction: P-selectin triggers protein-tyrosine phosphorylation-dependent CD11b/CD18 adhesion: role of PSGL-1 as a signaling molecule. Blood 280 9920836
2003 Interaction of P-selectin and PSGL-1 generates microparticles that correct hemostasis in a mouse model of hemophilia A. Nature medicine 248 12858167
2007 Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44. Immunity 237 17442598
2004 Role of P-selectin and PSGL-1 in coagulation and thrombosis. Thrombosis and haemostasis 186 15351841
2005 Recruitment of adult thymic progenitors is regulated by P-selectin and its ligand PSGL-1. Nature immunology 182 15880112
2002 6-Sulfo LacNAc, a novel carbohydrate modification of PSGL-1, defines an inflammatory type of human dendritic cells. Immunity 174 12354382
2002 ITAM-based interaction of ERM proteins with Syk mediates signaling by the leukocyte adhesion receptor PSGL-1. Immunity 173 12387735
2010 E-selectin engages PSGL-1 and CD44 through a common signaling pathway to induce integrin alphaLbeta2-mediated slow leukocyte rolling. Blood 169 20299514
2008 PSGL-1 engagement by E-selectin signals through Src kinase Fgr and ITAM adapters DAP12 and FcR gamma to induce slow leukocyte rolling. The Journal of experimental medicine 168 18794338
2007 Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling. Blood 142 17132726
2003 PSGL-1 participates in E-selectin-mediated progenitor homing to bone marrow: evidence for cooperation between E-selectin ligands and alpha4 integrin. Blood 140 12763924
2009 PSGL-1 function in immunity and steady state homeostasis. Immunological reviews 139 19594630
1996 P-selectin glycoprotein ligand-1 (PSGL-1) is a ligand for L-selectin in neutrophil aggregation. Blood 139 8839831
2016 PSGL-1 Is an Immune Checkpoint Regulator that Promotes T Cell Exhaustion. Immunity 131 27192578
2012 PSGL-1/selectin and ICAM-1/CD18 interactions are involved in macrophage-induced drug resistance in myeloma. Leukemia 131 22996336
2014 VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells. The Journal of experimental medicine 127 25135296
2017 PSGL-1: A New Player in the Immune Checkpoint Landscape. Trends in immunology 118 28262471
2002 Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters. Biophysical journal 111 11916843
2007 PSGL-1-mediated activation of EphB4 increases the proangiogenic potential of endothelial progenitor cells. The Journal of clinical investigation 106 17510705
2000 Intracellular parasitism by the human granulocytic ehrlichiosis bacterium through the P-selectin ligand, PSGL-1. Science (New York, N.Y.) 105 10834846
2000 Activation of human leukocytes reduces surface P-selectin glycoprotein ligand-1 (PSGL-1, CD162) and adhesion to P-selectin in vitro. Journal of immunology (Baltimore, Md. : 1950) 105 10946308
2007 Interaction of the selectin ligand PSGL-1 with chemokines CCL21 and CCL19 facilitates efficient homing of T cells to secondary lymphoid organs. Nature immunology 102 17401367
2013 Enterovirus 71 binding to PSGL-1 on leukocytes: VP1-145 acts as a molecular switch to control receptor interaction. PLoS pathogens 101 23935488
2000 Interleukin-13 induces PSGL-1/P-selectin-dependent adhesion of eosinophils, but not neutrophils, to human umbilical vein endothelial cells under flow. Blood 97 10807781
2013 The PSGL-1-L-selectin signaling complex regulates neutrophil adhesion under flow. The Journal of experimental medicine 92 24127491
2018 Cooperative PSGL-1 and CXCR2 signaling in neutrophils promotes deep vein thrombosis in mice. Blood 90 30068506
2008 Separable requirements for cytoplasmic domain of PSGL-1 in leukocyte rolling and signaling under flow. Blood 89 18550846
2017 The Interaction of Selectins and PSGL-1 as a Key Component in Thrombus Formation and Cancer Progression. BioMed research international 83 28680883
2000 Sialyl Lewis(x)-mediated, PSGL-1-independent rolling adhesion on P-selectin. Biophysical journal 77 10920004
2002 Attachment of the PSGL-1 cytoplasmic domain to the actin cytoskeleton is essential for leukocyte rolling on P-selectin. Blood 75 12036880
2009 PSGL-1-dependent myeloid leukocyte activation. Journal of leukocyte biology 73 19703898
2007 PSGL-1 regulates platelet P-selectin-mediated endothelial activation and shedding of P-selectin from activated platelets. Thrombosis and haemostasis 67 17938805
2004 Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII. The Journal of biological chemistry 64 15579466
2019 Proteomic profiling of HIV-1 infection of human CD4+ T cells identifies PSGL-1 as an HIV restriction factor. Nature microbiology 62 30833724
1999 Structure and function of the selectin ligand PSGL-1. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 62 10412562
2010 Tyrosine sulfation of the amino terminus of PSGL-1 is critical for enterovirus 71 infection. PLoS pathogens 58 21079683
2007 PSGL-1 and mTOR regulate translation of ROCK-1 and physiological functions of macrophages. The EMBO journal 58 17245434
2002 Molecular basis of leukocyte rolling on PSGL-1. Predominant role of core-2 O-glycans and of tyrosine sulfate residue 51. The Journal of biological chemistry 58 12403782
2002 Identification of CD16-2, a novel mouse receptor homologous to CD16/Fc gamma RIII. Immunogenetics 56 12389094
2020 PSGL-1 restricts HIV-1 infectivity by blocking virus particle attachment to target cells. Proceedings of the National Academy of Sciences of the United States of America 54 32273392
2010 Primary effusion lymphoma: genomic profiling revealed amplification of SELPLG and CORO1C encoding for proteins important for cell migration. The Journal of pathology 52 20690162
2009 Flotillins interact with PSGL-1 in neutrophils and, upon stimulation, rapidly organize into membrane domains subsequently accumulating in the uropod. PloS one 50 19404397
2001 differential L-selectin binding activities of human hematopoietic cell L-selectin ligands, HCELL and PSGL-1. The Journal of biological chemistry 49 11591704
2013 Caveolar endocytosis is required for human PSGL-1-mediated enterovirus 71 infection. Journal of virology 48 23760234
1998 Dimerization of P-selectin glycoprotein ligand-1 (PSGL-1) required for optimal recognition of P-selectin. The Journal of cell biology 48 9660879
2004 Immunoblockade of PSGL-1 attenuates established experimental murine colitis by reduction of leukocyte rolling. American journal of physiology. Gastrointestinal and liver physiology 46 15001428
2021 PSGL-1 Immune Checkpoint Inhibition for CD4+ T Cell Cancer Immunotherapy. Frontiers in immunology 45 33708224
2019 The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of dendritic cells by the TLR4 signaling pathway. Cell death & disease 45 31263109
2008 Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status. Arteriosclerosis, thrombosis, and vascular biology 44 18497306
2003 Murine neutrophils require alpha1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum. Blood 44 12869507
1996 A human colon carcinoma cell line exhibits adhesive interactions with P-selectin under fluid flow via a PSGL-1-independent mechanism. The American journal of pathology 43 8909255
2014 PSGL-1 and E/P-selectins are essential for T-cell rolling in inflamed CNS microvessels but dispensable for initiation of EAE. European journal of immunology 41 24740164
2001 Tyrosine sulfation enhances but is not required for PSGL-1 rolling adhesion on P-selectin. Biophysical journal 41 11566773
2014 Distribution of EV71 receptors SCARB2 and PSGL-1 in human tissues. Virus research 40 24997419
2023 Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages. Theranostics 39 37064877
2021 A PSGL-1 glycomimetic reduces thrombus burden without affecting hemostasis. Blood 39 33945603
2003 SELPLG gene polymorphisms in relation to plasma SELPLG levels and coronary artery disease. Annals of human genetics 39 14641238
2001 Neutrophils, monocytes, and dendritic cells express the same specialized form of PSGL-1 as do skin-homing memory T cells: cutaneous lymphocyte antigen. Biochemical and biophysical research communications 39 11453631
2020 Acute Myeloid Leukemia Chemo-Resistance Is Mediated by E-selectin Receptor CD162 in Bone Marrow Niches. Frontiers in cell and developmental biology 38 32793603
2015 The Suramin Derivative NF449 Interacts with the 5-fold Vertex of the Enterovirus A71 Capsid to Prevent Virus Attachment to PSGL-1 and Heparan Sulfate. PLoS pathogens 37 26430888
2005 Signaling function of PSGL-1 in neutrophil: tyrosine-phosphorylation-dependent and c-Abl-involved alteration in the F-actin-based cytoskeleton. Journal of cellular biochemistry 36 15526280
2003 Peritoneal macrophages express both P-selectin and PSGL-1. The Journal of cell biology 36 14662752
2007 Structural basis of PSGL-1 binding to ERM proteins. Genes to cells : devoted to molecular & cellular mechanisms 34 18076570
2015 Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites. Biochemistry 33 26115234
2012 Ezrin-radixin-moesin-binding sequence of PSGL-1 glycoprotein regulates leukocyte rolling on selectins and activation of extracellular signal-regulated kinases. The Journal of biological chemistry 33 22311979
2016 Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity. Scientific reports 32 27892504
2020 Virion-incorporated PSGL-1 and CD43 inhibit both cell-free infection and transinfection of HIV-1 by preventing virus-cell binding. Proceedings of the National Academy of Sciences of the United States of America 31 32193343
2015 Inhibition of P-Selectin and PSGL-1 Using Humanized Monoclonal Antibodies Increases the Sensitivity of Multiple Myeloma Cells to Bortezomib. BioMed research international 31 26539491
2003 Exogenous eosinophil activation converts PSGL-1-dependent binding to CD18-dependent stable adhesion to platelets in shear flow. American journal of physiology. Cell physiology 31 12529243
2023 PSGL-1 attenuates early TCR signaling to suppress CD8+ T cell progenitor differentiation and elicit terminal CD8+ T cell exhaustion. Cell reports 30 37115668
2013 Metastatic growth progression caused by PSGL-1-mediated recruitment of monocytes to metastatic sites. Cancer research 30 24322980
2004 Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. The Journal of biological chemistry 30 15466853
2014 Basic motifs target PSGL-1, CD43, and CD44 to plasma membrane sites where HIV-1 assembles. Journal of virology 29 25320329
2010 NF kappaB activation in embryonic endothelial progenitor cells enhances neovascularization via PSGL-1 mediated recruitment: novel role for LL37. Stem cells (Dayton, Ohio) 28 20014279
2022 Targeting the PSGL-1 Immune Checkpoint Promotes Immunity to PD-1-Resistant Melanoma. Cancer immunology research 26 35303066
2019 Acute Myeloid and Lymphoblastic Leukemia Cell Interactions with Endothelial Selectins: Critical Role of PSGL-1, CD44 and CD43. Cancers 26 31461905
2008 Surprising up-regulation of P-selectin glycoprotein ligand-1 (PSGL-1) in endotoxin-induced uveitis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26 19050046
2005 P-selectin glycoprotein ligand-1 (PSGL-1) is up-regulated on leucocytes from patients with chronic obstructive pulmonary disease. Clinical and experimental immunology 26 16232226
2004 Endotoxin down-modulates P-selectin glycoprotein ligand-1 (PSGL-1, CD162) on neutrophils in humans. Journal of clinical immunology 25 14997035
2012 PSGL-1 regulates the migration and proliferation of CD8(+) T cells under homeostatic conditions. Journal of immunology (Baltimore, Md. : 1950) 23 22250093
2006 Multi-enzyme one-pot strategy for the synthesis of sialyl Lewis X-containing PSGL-1 glycopeptide. Carbohydrate research 23 16762328
1998 Characterization of the O-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1). Glycoconjugate journal 23 10211703
2020 PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity. Viruses 22 33396594
2008 SLIC-1/sorting nexin 20: a novel sorting nexin that directs subcellular distribution of PSGL-1. European journal of immunology 22 18196517
2005 Biomechanics of P-selectin PSGL-1 bonds: shear threshold and integrin-independent cell adhesion. Biophysical journal 22 16387772
2021 High expression of neutrophil and monocyte CD64 with simultaneous lack of upregulation of adhesion receptors CD11b, CD162, CD15, CD65 on neutrophils in severe COVID-19. Therapeutic advances in infectious disease 21 34377464
2021 GALNT4 primes monocytes adhesion and transmigration by regulating O-Glycosylation of PSGL-1 in atherosclerosis. Journal of molecular and cellular cardiology 20 34974060
2020 PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteins. Cell discovery 20 32802403
2011 Tyrosine sulfation of native mouse Psgl-1 is required for optimal leukocyte rolling on P-selectin in vivo. PloS one 20 21633705
2004 A down-regulatable E-selectin ligand is functionally important for PSGL-1-independent leukocyte-endothelial cell interactions. Blood 20 15304396
2017 Soluble P-selectin rescues mice from anthrax lethal toxin-induced mortality through PSGL-1 pathway-mediated correction of hemostasis. Virulence 19 28102766
2008 Anaplasma phagocytophilum PSGL-1-independent infection does not require Syk and leads to less efficient AnkA delivery. Cellular microbiology 19 18485118
2002 Increased PSGL-1 expression on granulocytes from allergic-asthmatic subjects results in enhanced leukocyte recruitment under flow conditions. Journal of leukocyte biology 19 12377939
2007 Redistribution of P-selectin glycoprotein ligand-1 (PSGL-1) in chemokine-treated neutrophils: a role of lipid microdomains. Journal of leukocyte biology 18 17372146
1998 Cloning and sequencing of a cyclodextrin glycosyltransferase gene from Brevibacillus brevis CD162 and its expression in Escherichia coli. FEMS microbiology letters 18 9682490
2005 Engagement of PSGL-1 upregulates CSF-1 transcription via a mechanism that may involve Syk. Cellular immunology 17 16289055