Affinage

SELP

P-selectin · UniProt P16109

Length
830 aa
Mass
90.8 kDa
Annotated
2026-06-10
100 papers in source corpus 34 papers cited in narrative 30 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

P-selectin (SELP/GMP-140/CD62P/PADGEM) is a Ca2+-dependent lectin that mediates the earliest adhesive interactions between activated platelets or endothelium and circulating leukocytes, initiating inflammatory and thrombotic cell recruitment (PMID:2478294, PMID:1688717, PMID:1689464). It is a multi-domain transmembrane glycoprotein with an N-terminal C-type lectin domain, an EGF-like domain, nine complement-binding consensus repeats, a transmembrane segment, and a short cytoplasmic tail; alternative splicing additionally yields a soluble form lacking the transmembrane domain (PMID:2466574, PMID:2463989). In resting cells P-selectin is sequestered in platelet alpha-granules and endothelial Weibel-Palade bodies, and upon stimulation by agonists such as thrombin, histamine, or complement C5b-9 it is rapidly mobilized to the plasma membrane (PMID:2411738, PMID:2472431, PMID:2467701, PMID:2470750); this regulated storage is governed by its cytoplasmic domain, which is both necessary and sufficient to sort the protein into secretory granules (PMID:1378326). ADP-driven mobilization additionally requires combined P2Y1- and P2Y12-receptor signaling through PLC/IP3 and PI3K (PMID:33255391). The lectin domain binds sialylated, fucosylated carbohydrate ligands—principally sialyl Lewis x—and sulfatides, with key contact residues Lys113, Tyr48, and Tyr94 lining a shallow binding groove (PMID:1712483, PMID:1717488, PMID:1717159, PMID:7681324, PMID:7508745); it recognizes a sialylated glycoprotein counter-receptor on myeloid cells with nanomolar affinity, and L-selectin presents sialyl Lewis x as one such ligand (PMID:1704009, PMID:1378449, PMID:1716182). Through these interactions P-selectin tethers neutrophils, monocytes, memory T cells, and NK cells, cooperating with PAF to license CD18 integrin-dependent firm adhesion, triggering leukocyte oxidative burst, and driving tissue factor expression during venous thrombus resolution (PMID:1717478, PMID:7690799, PMID:1378721, PMID:38385292). P-selectin also complexes with TLR4 to bind bacterial LPS and promote platelet activation (PMID:16514062). The soluble monomeric plasma form binds the same neutrophil receptor yet exerts anti-inflammatory effects, inhibiting CD18-dependent neutrophil adhesion and superoxide production (PMID:1696029, PMID:1706523, PMID:1372646).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 1985 High

    Established that P-selectin is a stored, mobilizable adhesion molecule rather than a constitutive surface protein, defining the regulated-secretion logic central to its function.

    Evidence Immunogold electron microscopy of resting and thrombin-stimulated platelets

    PMID:2411738

    Open questions at the time
    • Did not define the molecular sorting signal
    • Did not identify ligands or adhesive function
  2. 1989 High

    Defined the multi-domain primary structure (lectin-EGF-CR-TM-cytoplasmic) and the existence of a soluble splice variant, providing the structural framework for all downstream mechanism.

    Evidence cDNA cloning and sequence analysis; amino acid composition and deglycosylation biochemistry

    PMID:2463989 PMID:2466574

    Open questions at the time
    • Domain functions not assigned experimentally
    • No ligand identified at this stage
  3. 1989 High

    Extended P-selectin storage and inducible surface display to endothelium, establishing it as a shared platelet/endothelial adhesion receptor mobilized by distinct agonists including complement.

    Evidence Immunoperoxidase/immunogold EM with vWF co-localization, metabolic labeling, histamine and C5b-9 stimulation

    PMID:2467701 PMID:2470750 PMID:2472431

    Open questions at the time
    • Signaling pathways for translocation only partially defined
    • Leukocyte counter-receptors unknown
  4. 1989 High

    Demonstrated that P-selectin mediates Ca2+-dependent adhesion of activated platelets to leukocytes, defining its core adhesive activity and distinguishing it from other platelet adhesion molecules.

    Evidence Cell adhesion and lipid-vesicle binding assays with antibody and EDTA blocking

    PMID:1688717 PMID:2478294

    Open questions at the time
    • Molecular nature of the leukocyte ligand not yet known
    • No structural basis for binding
  5. 1990 High

    Showed via transfection that endothelial P-selectin alone is sufficient to mediate neutrophil adhesion, proving cell-autonomous adhesive function independent of leukocyte metabolism.

    Evidence COS cell transfection, purified-protein coating, antibody blocking

    PMID:1689464

    Open questions at the time
    • Ligand identity still pending
    • Did not address subsequent firm adhesion
  6. 1990 High

    Identified carbohydrate (Lewis x / CD15) as part of the ligand, opening the carbohydrate-recognition mechanism of P-selectin.

    Evidence Antibody and free-carbohydrate inhibition of adhesion, transfectant assays

    PMID:1699666

    Open questions at the time
    • Did not define the full glycan determinant
    • Protein scaffold of the ligand unidentified
  7. 1991 High

    Resolved the glycan determinant as sialyl Lewis x with required sialic acid and quantified high-affinity, Ca2+-dependent binding to a sialylated neutrophil glycoprotein, defining the biochemical ligand requirements.

    Evidence Purified-protein and radioligand binding, neuraminidase treatment, glycosyltransferase transfectants

    PMID:1704009 PMID:1712483 PMID:1717488

    Open questions at the time
    • Protein backbone of the neutrophil receptor not yet isolated
  8. 1991 High

    Biochemically identified the myeloid counter-receptor as a distinct ~250 kDa sialylated glycoprotein (not L-selectin or lamps), and separately showed L-selectin can present sialyl Lewis x to P-selectin.

    Evidence Radioligand blotting, affinity chromatography, immunodepletion; anti-LECAM-1 blocking and removal

    PMID:1378449 PMID:1716182

    Open questions at the time
    • Did not name the principal high-affinity ligand gene
    • Relative contribution of multiple ligands unresolved
  9. 1991 High

    Defined P-selectin's role in the leukocyte adhesion cascade: tethering leukocytes integrin-independently while cooperating with PAF to enable CD18-dependent firm adhesion.

    Evidence Adhesion assays with anti-P-selectin and anti-CD11/CD18 antibodies on stimulated endothelium

    PMID:1717478

    Open questions at the time
    • Precise signaling link between tethering and PAF response unresolved
  10. 1991 High

    Revealed an additional, non-sialyl-Lewis-x ligand class (sulfatides) and that oxidant stress and sulfated glycans modulate P-selectin engagement, broadening its ligand and regulatory repertoire.

    Evidence Chimera/soluble-protein binding to sulfatides, sulfated-glycan competition, oxidant-induced surface expression assays

    PMID:1704376 PMID:1706335 PMID:1717159

    Open questions at the time
    • Physiological relevance of sulfatide and sulfated-glycan binding in vivo not established
    • Site-proximity claim is pharmacological inference
  11. 1991 High

    Distinguished functional roles of membrane vs. soluble P-selectin: immobilized protein supports CD18-independent adhesion whereas soluble protein is anti-inflammatory, inhibiting CD18 adhesion and superoxide production.

    Evidence Adhesion and superoxide assays with immobilized vs. soluble protein, Fab blockade

    PMID:1696029 PMID:1706523

    Open questions at the time
    • Mechanism of soluble-form inhibition not defined
    • In vivo significance of soluble pool unaddressed here
  12. 1992 High

    Mapped the granule-sorting determinant to the cytoplasmic tail, showing it is necessary and sufficient to route cargo into the regulated secretory pathway.

    Evidence Transfection, tail-deletion mutants, and tissue-factor chimeras with immunogold EM in AtT20/COS/CHO cells

    PMID:1378326

    Open questions at the time
    • Cytosolic machinery recognizing the tail not identified
  13. 1992 High

    Identified the lectin-domain residues (Lys113, Tyr48, Tyr94) forming the carbohydrate-binding groove and showed their exact stereochemistry is essential, providing the structural basis of ligand recognition.

    Evidence Homology modeling and site-directed mutagenesis with cell-binding readout; conservative substitutions

    PMID:7508745 PMID:7681324

    Open questions at the time
    • No experimental crystal structure of P-selectin in this corpus
    • Overlap of myeloid and sulfatide sites inferred from mutants
  14. 1992 Medium

    Established P-selectin's effector and broader cellular reach: it triggers monocyte/neutrophil superoxide release on contact, binds memory T-cell and NK subsets, modulates T-cell cytokines, recognizes a distinct carcinoma-cell ligand, and circulates as a functional plasma monomer.

    Evidence Superoxide assays, flow cytometry with purified/fusion protein, neuraminidase and sulfated-glycan dependence, plasma purification and gel filtration

    PMID:1372439 PMID:1372646 PMID:1378017 PMID:1378721 PMID:7690799

    Open questions at the time
    • Tumor-cell ligand identity unresolved
    • Lymphocyte counter-receptors not biochemically defined
  15. 2006 Medium

    Linked P-selectin to innate immune sensing by showing it complexes with TLR4 to bind bacterial LPS and promote platelet activation.

    Evidence Co-localization, TLR4-knockout mice, LPS binding and platelet activation readouts

    PMID:16514062

    Open questions at the time
    • Stoichiometry and structural basis of the P-selectin–TLR4 complex unknown
    • Single lab
  16. 2020 Medium

    Dissected the platelet signaling required for surface P-selectin display, showing dual P2Y1/P2Y12 input converging on PLC/IP3-driven Ca2+ and PI3K activation.

    Evidence Selective receptor antagonists and pathway inhibitors with flow cytometry and Akt phosphorylation in platelets

    PMID:33255391

    Open questions at the time
    • Pharmacological inference; downstream granule-fusion machinery not defined
    • Single lab
  17. 2024 Medium

    Defined an in vivo role for platelet P-selectin in venous thrombus resolution, where it activates infiltrating neutrophils and monocytes, drives oxidative burst and tissue factor, and restrains fibrinolysis.

    Evidence Mouse IVC stenosis model with P-selectin blocking antibody, intravital imaging, leukocyte-aggregate and uPA/tissue-factor analyses

    PMID:38385292

    Open questions at the time
    • Counter-receptor on infiltrating leukocytes not specified
    • Single lab; antibody-based loss-of-function

Open questions

Synthesis pass · forward-looking unresolved questions
  • The identity of the principal high-affinity physiological glycoprotein counter-receptor(s) and the cytosolic sorting machinery recognizing the P-selectin cytoplasmic tail remain unresolved in this corpus.
  • Named myeloid counter-receptor gene not identified in the timeline
  • Tail-binding adaptors unknown
  • No experimental high-resolution structure of P-selectin captured here

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098631 cell adhesion mediator activity 3 GO:0008289 lipid binding 1
Localization
GO:0005886 plasma membrane 3 GO:0031410 cytoplasmic vesicle 3 GO:0005576 extracellular region 1
Pathway
R-HSA-168256 Immune System 3 R-HSA-109582 Hemostasis 2 R-HSA-1500931 Cell-Cell communication 2
Partners
P2Y1P2Y12SELLTLR4

Evidence

Reading pass · 30 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1985 GMP-140 (SELP) is stored in alpha-granule membranes of resting platelets and is rapidly redistributed to the plasma membrane upon thrombin stimulation, as shown by immunocytochemical localization with immunogold probes on frozen thin sections and permeabilized platelets. Immunocytochemistry (immunogold electron microscopy, saponin permeabilization, polyclonal antibody labeling) The Journal of cell biology High 2411738
1989 GMP-140 (SELP) cDNA-derived primary structure predicts a multi-domain protein with an N-terminal lectin domain, an EGF-like domain, nine tandem complement-binding consensus repeats, a transmembrane domain, and a short cytoplasmic tail; some cDNAs also predict a soluble alternatively spliced form lacking the transmembrane domain. cDNA cloning and sequence analysis Cell High 2466574
1989 GMP-140 (SELP) is synthesized by vascular endothelial cells and localizes to Weibel-Palade bodies (co-localizing with von Willebrand factor), from which it redistributes to the plasma membrane upon histamine stimulation. Immunoperoxidase tissue staining, [35S]cysteine metabolic labeling, double-label immunogold electron microscopy on frozen thin sections, cell stimulation assay The Journal of clinical investigation High 2467701 2472431
1989 PADGEM/GMP-140 (SELP) on activated platelets mediates Ca2+-dependent adhesion to neutrophils, monocytes, HL60 cells, and U937 cells; this interaction is blocked by anti-PADGEM antibodies and EDTA but not by antibodies to GPIIb-IIIa, thrombospondin, or GPIV. Cell adhesion assay, antibody blocking, lipid vesicle binding assay, EDTA inhibition Cell High 1688717 2478294
1989 GMP-140 (SELP) is a cysteine-rich, heavily glycosylated protein (28.8% carbohydrate by weight, predominantly N-linked); N-linked oligosaccharides account for more than 50 kDa of apparent molecular weight, and the bulk of the protein mass is extracytoplasmic. Amino acid composition analysis, enzymatic deglycosylation (PNGase/Endo H), pulse-chase biosynthetic labeling in HEL cells, protease surface accessibility assay The Journal of biological chemistry High 2463989
1990 GMP-140 (SELP) expressed on the surface of activated endothelial cells mediates rapid adhesion of neutrophils; this adhesion requires extracellular Ca2+ but not active neutrophil metabolism, and is inhibited by anti-GMP-140 antibodies. COS cell transfection with GMP-140 cDNA, cell adhesion assay, antibody blocking, purified protein coating on microtiter plates Nature High 1689464
1990 PADGEM/GMP-140 (SELP) recognizes the carbohydrate antigen CD15 (lacto-N-fucopentaose III, Lewis x) on neutrophils and monocytes as part of its ligand; anti-CD15 antibodies and the free carbohydrate inhibit PADGEM-mediated cell adhesion. Antibody blocking assay, carbohydrate inhibition of cell adhesion, COS cell transfection adhesion assay Cell High 1699666
1991 GMP-140 (SELP) co-expressed with platelet-activating factor (PAF) on histamine- or thrombin-stimulated endothelial cells acts cooperatively: GMP-140 tethers PMNs to the endothelium without requiring CD11/CD18 integrin activation, while PAF activates CD11/CD18-dependent firm adhesion. GMP-140 tethering potentiates PMN response to PAF by a mechanism distal to the PAF receptor. Cell adhesion assay, antibody blocking (anti-GMP-140, anti-CD11/CD18), soluble GMP-140 competition, histamine/thrombin EC stimulation The Journal of cell biology High 1717478
1991 Neutrophil L-selectin (LECAM-1) is modified with sialyl Lewis x and presents this oligosaccharide ligand to GMP-140 (SELP) on activated endothelium; LECAM-1 is concentrated on PMN microvilli. Anti-LECAM-1 antibodies or removal of LECAM-1 inhibits PMN binding to GMP-140 transfectants by up to 70%. Flow cytometry, anti-LECAM-1 antibody blocking, selective cell-surface removal of LECAM-1, adhesion assay on GMP-140-transfected cells Cell High 1716182
1991 GMP-140 (SELP; CD62) and ELAM-1 both recognize sialyl Lewis x (Neu5Ac-α2-3-Gal-β1-4-(Fuc-α1-3)-GlcNAc) as a carbohydrate ligand on myeloid and non-myeloid cells; sialic acid is required for recognition. Binding assay with purified GMP-140, cells expressing cloned glycosyltransferases, neuraminidase treatment, sialyl Lewis x-expressing cell lines Proceedings of the National Academy of Sciences of the United States of America High 1712483 1717488
1991 GMP-140 (SELP) binds to a protease-sensitive, sialic acid-containing glycoprotein receptor on neutrophils in a Ca2+-dependent, reversible, and saturable manner (Kd ~1.5 nM, ~3–6 nM for saturation). Treatment with neuraminidase (cleaving α2-3 and α2-8 sialic acids) diminishes binding, implicating sialylated glycoprotein as receptor. Radioligand binding assay ([125I]-GMP-140), protease treatment, neuraminidase treatment, Ca2+ chelation, PMA stimulation The Journal of cell biology High 1704009
1991 Oxygen radicals (H2O2, t-butylhydroperoxide, menadione) induce prolonged surface expression of GMP-140 (SELP) on endothelial cells, which mediates PMN adhesion; this occurs without new protein synthesis and requires intracellular iron (inhibited by iron chelators). Anti-GMP-140 antibody or soluble GMP-140 completely blocks PMN adhesion to oxidant-treated endothelium. PMN adhesion assay, antibody blocking, protein synthesis inhibition, antioxidant and iron chelator treatment, flow cytometry for GMP-140 surface expression The Journal of cell biology High 1704376
1991 CD62/GMP-140 (SELP) binds sulfatides (3-sulfated galactosyl ceramides) on granulocytes and tumor cell plasma membranes; this represents a distinct ligand from sialyl Lewis x. Granulocytes excrete sulfatides, potentially facilitating disengagement from CD62 upon exiting the bloodstream. Binding assay with soluble CD62-IgG fusion protein, sulfatide-coated plastic, tumor cell and granulocyte adhesion assay Cell High 1717159
1991 GMP-140 (SELP) binding to neutrophils is inhibited by sulfated glycans (heparin, fucoidan, dextran sulfate-500,000) but not by chondroitin sulfates. Fluid-phase GMP-140 exists predominantly as a tetramer. This suggests that the sulfated glycan binding site and the neutrophil receptor binding site on GMP-140 are identical or proximal. Radioligand binding assay ([125I]-GMP-140), rosetting assay, sulfated glycan competition The Journal of biological chemistry Medium 1706335
1991 GMP-140 (SELP) is also present on platelet dense granule membranes (in addition to alpha-granules), as shown by immunoelectron microscopy double-labeling with granulophysin; after thrombin stimulation, GMP-140 redistributes from dense granule membranes to the plasma membrane. Immunoelectron microscopy (immunogold), double-labeling with anti-GMP-140 and anti-granulophysin antibodies, serotonin release assay Blood Medium 1377048
1991 Soluble (fluid-phase) GMP-140 (SELP) inhibits CD18-dependent adhesion of TNF-α-activated neutrophils to resting endothelium, while immobilized GMP-140 supports neutrophil adhesion independently of CD18; soluble GMP-140 also inhibits superoxide anion production by neutrophils stimulated with TNF-α or fMLP. Neutrophil adhesion assay on coated plastic or endothelium, superoxide anion measurement, soluble protein competition, anti-GMP-140 Fab blockade Science; Proceedings of the National Academy of Sciences High 1696029 1706523
1991 GMP-140 (SELP) binds to a glycoprotein ligand on myeloid cells (apparent Mr ~250,000 non-reduced, ~120,000 reduced) identified by [125I]-P-selectin blotting and affinity chromatography; binding is Ca2+-dependent, blocked by anti-P-selectin mAbs, sialidase-sensitive (prolonged digestion abolishes binding), and the ligand is not L-selectin, lamp-1, lamp-2, or leukosialin. Radioligand blotting ([125I]-P-selectin), affinity chromatography, neuraminidase treatment, immunodepletion, Western blot The Journal of cell biology High 1378449
1992 The cytoplasmic domain of P-selectin (SELP) is both necessary and sufficient for sorting into the regulated secretory pathway. The last 23 amino acids of the 35-residue cytoplasmic tail are required; deletion of this region re-routes P-selectin to the plasma membrane in AtT20 cells. Replacement of the cytoplasmic tail of tissue factor with the P-selectin cytoplasmic domain redirects tissue factor to secretory granules. cDNA transfection into AtT20, COS-7, and CHO cells; immunogold electron microscopy; cytoplasmic tail deletion mutants; chimeric protein construction; agonist (8-Br-cAMP) stimulation Molecular biology of the cell High 1378326
1992 Key residues in the lectin domain of P-selectin (SELP) required for myeloid cell binding are Lys113, Tyr48, and Tyr94, located in a shallow groove formed by the β2, β3, and β5 strands. Single alanine or phenylalanine substitutions at these positions abolish binding to myeloid cells. 3D homology modeling based on rat mannose-binding protein crystal structure, site-directed mutagenesis, cell binding assay Biochemistry High 7681324
1992 Soluble GMP-140 (SELP) circulates as a monomer in plasma (not tetramer as in platelet membrane form), is detectable at ~0.2–0.25 µg/mL in normal plasma, and binds the same neutrophil receptor as membrane-bound GMP-140 with equivalent functional activity. Its plasma origin is consistent with alternative mRNA splicing to produce a form lacking the transmembrane domain. Ultracentrifugation (100,000g), ELISA, protein purification from plasma, analytical gel filtration, neutrophil binding assay The Journal of experimental medicine High 1372646
1992 P-selectin (SELP) on activated platelets induces superoxide anion release by monocytes and neutrophils; this effect is inhibited by anti-P-selectin antibody, anti-sialyl-Lewis X antibody, or soluble recombinant P-selectin, and requires membrane contact (not soluble platelet secretion products). Superoxide anion measurement, thrombin-activated platelet membranes, antibody blocking, soluble recombinant P-selectin competition Journal of immunology Medium 7690799
1992 P-selectin (SELP) binds to a glycoprotein ligand on carcinoma cells (colon, lung, breast) that is distinct from the myeloid cell ligand: neuraminidase treatment of a breast carcinoma cell line does not abolish GMP-140 binding (unlike HL-60 cells where sialic acid is essential), indicating tumor cells present a different P-selectin ligand. Immunohistology with GMP-140-IgG chimera on tissue sections, neuraminidase treatment, cell-line binding assay Proceedings of the National Academy of Sciences of the United States of America Medium 1372439
1992 P-selectin (SELP; CD62) binds to subpopulations of memory T lymphocytes (CD45RO+ CD4+ and CD8+) and NK cells (CD16+) in a Ca2+-dependent, sialidase-sensitive manner, and can mediate adhesion of activated platelets to these lymphocyte subsets. Multi-color flow cytometry with purified P-selectin, Ca2+ dependence, neuraminidase treatment, platelet-lymphocyte rosetting assay Biochemical and biophysical research communications Medium 1378721
1992 P-selectin (SELP) binds to chronically antigen-stimulated CD4+ T cells (but not freshly isolated CD4+ T cells); binding requires sialic acid (abolished by neuraminidase) and is Ca2+-dependent, and is blocked by dextran sulfate, fucoidan, and heparin. P-selectin modulates cytokine production (augments GM-CSF, inhibits IL-8) in primed T cells. P-selectin-IgG fusion protein binding assay by flow cytometry, neuraminidase treatment, sulfated glycan inhibition, cytokine measurement (ELISA) European journal of immunology Medium 1378017
1994 The myeloid cell binding site and the sulfatide binding site of P-selectin (SELP) lectin domain are overlapping; conservative substitutions Tyr48Ser or Lys113Arg produce correctly folded P-selectin mutants that fail to bind HL-60 cells, establishing that the exact stereochemistry of these residues is critical. Site-directed mutagenesis, cell binding assay (HL-60 cells), sulfatide binding assay Biochemistry High 7508745
1989 Complement C5b-9 triggers translocation of GMP-140 (SELP) to the endothelial cell surface and secretion of vWF; this requires C9 deposition, causes Ca2+ influx from external medium, and is partially inhibited by the protein kinase inhibitor sphingosine. C5b-9 complement deposition assay, immunofluorescence for GMP-140 surface expression, cytosolic Ca2+ measurement, C8-deficient serum controls, protein kinase inhibition The Journal of biological chemistry Medium 2470750
2016 MicroRNAs miR-26b and miR-140 directly regulate SELP mRNA levels in megakaryocytes; experimental overexpression or inhibition of these miRNAs in MEG-01 cells correspondingly changes SELP mRNA levels. Hyperglycemia suppresses these miRNAs, leading to elevated SELP mRNA and increased platelet P-selectin surface expression. miRNA mimic/anti-miRNA transfection in MEG-01 megakaryocytic cells, RT-qPCR for SELP mRNA, flow cytometry for surface P-selectin, calpain inhibition to restore miRNA Thrombosis and haemostasis Medium 27975100
2020 ADP-activated expression of CD62P (P-selectin/SELP) from platelet granules requires co-activation of both P2Y1 and P2Y12 receptors. The P2Y12 receptor activates PI3K (demonstrated by Akt1 phosphorylation assay), while P2Y1 activation creates high cytosolic Ca2+ required for optimal PI3K activity. Inhibition of PLC, IP3 receptor, or PI3K (wortmannin) almost completely blocks ADP-induced CD62P surface expression. Selective P2Y1/P2Y12 receptor antagonists (MRS2500, PSB0739), pharmacological inhibitors (U73122, 2-APB, calmidazolium, wortmannin), flow cytometry for CD62P, Akt1 phosphorylation assay in platelet lysates Pharmaceuticals Medium 33255391
2024 P-selectin (SELP/CD62P) on platelets activates infiltrating neutrophils and Ly6Chigh monocytes during venous thrombus resolution; P-selectin blocking antibody reduces platelet-neutrophil and platelet-monocyte aggregates, decreases oxidative burst in the thrombus, lowers tissue factor expression in the vessel wall, reduces thrombus density, and increases urokinase-type plasminogen activator levels, accelerating fibrin degradation and thrombus volume reduction. Mouse inferior vena cava stenosis model, P-selectin blocking antibody, flow cytometry for platelet-leukocyte aggregates, scanning electron microscopy, ROS measurement, tissue factor expression analysis, uPA quantification, intravital imaging Arteriosclerosis, thrombosis, and vascular biology Medium 38385292
2006 Platelet CD62P (P-selectin/SELP) forms a complex with TLR4 on the platelet membrane and cooperates with TLR4 in binding EHEC LPS to platelets, contributing to platelet activation; TLR4-deficient mice fail to bind O157LPS and show preserved platelet counts after LPS injection. Co-localization of TLR4 and CD62P by immunofluorescence, TLR4 knockout mice (C57BL/10ScN), in vitro and in vivo LPS binding, flow cytometry for CD40L and GPIIb/IIIa activation, perfusion assay on endothelial cells with TLR4/CD62 blocking antibodies Blood Medium 16514062

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1990 Rapid neutrophil adhesion to activated endothelium mediated by GMP-140. Nature 930 1689464
1989 GMP-140, a platelet alpha-granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodies. The Journal of clinical investigation 899 2472431
1989 PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes. Cell 814 2478294
1985 A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation. The Journal of cell biology 813 2411738
1989 Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation. Cell 756 2466574
1991 Oxygen radicals induce human endothelial cells to express GMP-140 and bind neutrophils. The Journal of cell biology 589 1704376
1991 Coexpression of GMP-140 and PAF by endothelium stimulated by histamine or thrombin: a juxtacrine system for adhesion and activation of neutrophils. The Journal of cell biology 580 1717478
1991 The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140. Cell 566 1716182
1990 GMP-140 mediates adhesion of stimulated platelets to neutrophils. Blood 515 1688717
1991 CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x. Proceedings of the National Academy of Sciences of the United States of America 511 1712483
1989 PADGEM (GMP140) is a component of Weibel-Palade bodies of human endothelial cells. Blood 495 2467701
1992 Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells. The Journal of cell biology 458 1378449
1992 Neutrophil-dependent acute lung injury. Requirement for P-selectin (GMP-140). The Journal of clinical investigation 376 1383277
1989 Complement proteins C5b-9 induce secretion of high molecular weight multimers of endothelial von Willebrand factor and translocation of granule membrane protein GMP-140 to the cell surface. The Journal of biological chemistry 366 2470750
1990 PADGEM-dependent adhesion of platelets to monocytes and neutrophils is mediated by a lineage-specific carbohydrate, LNF III (CD15). Cell 341 1699666
1991 CD62/P-selectin recognition of myeloid and tumor cell sulfatides. Cell 287 1717159
1990 Prevention of activated neutrophil adhesion to endothelium by soluble adhesion protein GMP140. Science (New York, N.Y.) 261 1696029
1992 Characterization of GMP-140 (P-selectin) as a circulating plasma protein. The Journal of experimental medicine 258 1372646
1991 GMP-140 binds to a glycoprotein receptor on human neutrophils: evidence for a lectin-like interaction. The Journal of cell biology 248 1704009
1993 Activated platelets induce superoxide anion release by monocytes and neutrophils through P-selectin (CD62). Journal of immunology (Baltimore, Md. : 1950) 201 7690799
1991 GMP-140: a receptor for neutrophils and monocytes on activated platelets and endothelium. Journal of cellular biochemistry 198 1711528
1993 Expression patterns of leukocyte adhesion ligand molecules on human liver endothelia. Lack of ELAM-1 and CD62 inducibility on sinusoidal endothelia and distinct distribution of VCAM-1, ICAM-1, ICAM-2, and LFA-3. The American journal of pathology 194 8434643
1991 The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells. The Journal of cell biology 181 1717488
1991 GMP-140 binding to neutrophils is inhibited by sulfated glycans. The Journal of biological chemistry 178 1706335
1990 Properties of GMP-140, an inducible granule membrane protein of platelets and endothelium. Blood cells 159 1693535
2004 CD4+ CD25+ CD62+ T-regulatory cell subset has optimal suppressive and proliferative potential. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 154 14678036
2006 Lipopolysaccharide from enterohemorrhagic Escherichia coli binds to platelets through TLR4 and CD62 and is detected on circulating platelets in patients with hemolytic uremic syndrome. Blood 150 16514062
1992 Cytoplasmic domain of P-selectin (CD62) contains the signal for sorting into the regulated secretory pathway. Molecular biology of the cell 144 1378326
1991 Adhesion protein GMP140 inhibits superoxide anion release by human neutrophils. Proceedings of the National Academy of Sciences of the United States of America 117 1706523
1992 GMP-140 (P-selectin/CD62) binds to chronically stimulated but not resting CD4+ T lymphocytes and regulates their production of proinflammatory cytokines. European journal of immunology 102 1378017
1992 Granule membrane protein 140 (GMP140) binds to carcinomas and carcinoma-derived cell lines. Proceedings of the National Academy of Sciences of the United States of America 100 1372439
1990 Requirement for sialic acid on neutrophils in a GMP-140 (PADGEM) mediated adhesive interaction with activated platelets. Biochemical and biophysical research communications 94 1700907
1992 P-selectin (CD62) binds to subpopulations of human memory T lymphocytes and natural killer cells. Biochemical and biophysical research communications 92 1378721
2007 Platelet surface CD62P and CD63, mean platelet volume, and soluble/platelet P-selectin as indexes of platelet function in atrial fibrillation: a comparison of "healthy control subjects" and "disease control subjects" in sinus rhythm. Journal of the American College of Cardiology 89 17498581
1989 Structural and biosynthetic studies of the granule membrane protein, GMP-140, from human platelets and endothelial cells. The Journal of biological chemistry 88 2463989
1995 Primitive human hematopoietic progenitors adhere to P-selectin (CD62P). Blood 86 7540063
1992 Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140). Blood 84 1377048
1989 Characterization of human platelet GMP-140 as a heparin-binding protein. Biochemical and biophysical research communications 77 2480118
2016 Hyperglycaemia suppresses microRNA expression in platelets to increase P2RY12 and SELP levels in type 2 diabetes mellitus. Thrombosis and haemostasis 72 27975100
1993 Interaction of P-selectin (CD62) and its cellular ligand: analysis of critical residues. Biochemistry 72 7681324
1994 CD62/P-selectin binding sites for myeloid cells and sulfatides are overlapping. Biochemistry 68 7508745
2005 Memory T lymphocytes generated by Mycobacterium bovis BCG vaccination reside within a CD4 CD44lo CD62 ligand(hi) population. Infection and immunity 63 16239580
2008 CD40L+ CD4+ memory T cells migrate in a CD62P-dependent fashion into reactive lymph nodes and license dendritic cells for T cell priming. The Journal of experimental medicine 62 18838544
1995 Oxidized low-density lipoprotein induces the expression of P-selectin (GMP140/PADGEM/CD62) on human endothelial cells. The Biochemical journal 59 7532399
1992 Adhesion molecule expression in Graves' thyroid glands; potential relevance of granule membrane protein (GMP-140) and intercellular adhesion molecule-1 (ICAM-1) in the homing and antigen presentation processes. Clinical and experimental immunology 59 1378365
1996 Plasma P selectin (GMP-140) and glycocalicin are elevated in preeclampsia and eclampsia: their significances. American journal of obstetrics and gynecology 57 8572020
1996 Flow cytometric analysis of platelet activation markers CD62P and CD63 in patients with coronary artery disease. European journal of clinical investigation 56 8957206
1991 Inhibition of platelet functions by a monoclonal antibody (LYP20) directed against a granule membrane glycoprotein (GMP-140/PADGEM). Blood 53 2015399
2002 P-selectin glycoprotein ligand-1 (rPSGL-Ig)-mediated blockade of CD62 selectin molecules protects rat steatotic liver grafts from ischemia/reperfusion injury. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 51 12201360
1993 PADGEM/GMP-140 expression on platelet membranes from homozygous beta thalassaemic patients. British journal of haematology 49 7687856
2002 Close relationship between the platelet activation marker CD62 and the granular release of platelet-derived growth factor. The Journal of pharmacology and experimental therapeutics 48 11861803
2000 Flow cytometric parameters for characterizing platelet activation by measuring P-selectin (CD62) expression: theoretical consideration and evaluation in thrombin-treated platelet populations. Biochemical and biophysical research communications 47 10694482
1995 Quantitation of CD62, soluble CD62, and lysosome-associated membrane proteins 1 and 2 for evaluation of the quality of stored platelet concentrates. Transfusion 47 7535481
1999 A flow cytometric assay of platelet activation marker P-selectin (CD62P) distinguishes heparin-induced thrombocytopenia (HIT) from HIT with thrombosis (HITT). Thrombosis and haemostasis 45 10544909
1995 A major role for CD62P/CD15s interaction in leukocyte margination during hemodialysis. Kidney international 45 7564097
1993 Fixation with formaldehyde induces expression of activation dependent platelet membrane glycoproteins, P selectin (CD62) and GP53 (CD63). British journal of haematology 45 7692932
1991 Enhancement by IL-1 beta and IFN-gamma of platelet activation: adhesion to leukocytes via GMP-140/PADGEM protein (CD62). Biochemical and biophysical research communications 45 1716887
1991 Downregulation of GMP-140 (CD62 or PADGEM) expression on platelets by N,N-dimethyl and N,N,N-trimethyl derivatives of sphingosine. Biochemistry 41 1721534
2008 Flow cytometric measurement of CD62P (P-selectin) expression on platelets: a multicenter optimization and standardization effort. Transfusion 40 18482194
2014 AKT1 and SELP polymorphisms predict the risk of developing cachexia in pancreatic cancer patients. PloS one 38 25238546
1986 Topographic distribution of a granule membrane protein (GMP-140) that is expressed on the platelet surface after activation: an immunogold-surface replica study. Blood cells 38 2431732
1993 Detection of plasma alpha-granule membrane protein GMP-140 using radiolabeled monoclonal antibodies in thrombotic diseases. Haemostasis 37 7690003
1991 Decreased content and surface expression of alpha-granule membrane protein GMP-140 in one of two types of platelet alpha delta storage pool deficiency. The Journal of clinical investigation 36 1705568
2004 Polymorphisms in the P-selectin (CD62P) and P-selectin glycoprotein ligand-1 (PSGL-1) genes and coronary heart disease. Clinical chemistry and laboratory medicine 34 15497463
2001 High-resolution CryoFESEM of individual cell adhesion molecules (CAMs) in the glycocalyx of human platelets: detection of P-selectin (CD62P), GPI-IX complex (CD42A/CD42B alpha,B beta), and integrin GPIIbIIIa (CD41/CD61) by immunogold labeling and stereo imaging. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 34 11410606
1991 Biochemical and immunohistochemical characteristics of CD62 and CD63 monoclonal antibodies. Expression of GMP-140 and LIMP-CD63 (CD63 antigen) in human lymphoid tissues. Virchows Archiv. B, Cell pathology including molecular pathology 33 1723556
2000 Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P). Tissue antigens 32 11019911
2005 CD62 blockade with P-Selectin glycoprotein ligand-immunoglobulin fusion protein reduces ischemia-reperfusion injury after rat intestinal transplantation. Transplantation 30 15714168
2000 Effect of glycoprotein IIb/IIIa inhibitors on CD62p expression, platelet aggregates, and microparticles in vitro. The Journal of laboratory and clinical medicine 30 10711863
2024 Reduced Monocyte and Neutrophil Infiltration and Activation by P-Selectin/CD62P Inhibition Enhances Thrombus Resolution in Mice. Arteriosclerosis, thrombosis, and vascular biology 27 38385292
1993 Removal by white cell-reduction filters of activated platelets expressing CD62. Transfusion 27 7505067
1995 Detection in human blood platelets of sialyl Lewis X gangliosides, potential ligands for CD62 and other selectins. Glycobiology 26 8563144
2018 Fabrication and Characterization of Recombinant Silk-Elastin-Like-Protein (SELP) Fiber. Macromolecular bioscience 25 30417967
2002 Adoptive immunotherapy of advanced tumors with CD62 L-selectin(low) tumor-sensitized T lymphocytes following ex vivo hyperexpansion. Journal of immunology (Baltimore, Md. : 1950) 24 12218152
1996 Expression of selectins (CD62 E,L,P) and cellular adhesion molecules in primary Sjögren's syndrome: questions to immunoregulation. Clinical immunology and immunopathology 24 8674240
2009 Regulation of CD40L (CD154) and CD62P (p-selectin) surface expression upon GPIIb-IIIa blockade of platelets from stable coronary artery disease patients. Thrombosis research 23 19487018
1994 Effect of cardiopulmonary bypass on the circulating level of soluble GMP-140. The Annals of thoracic surgery 23 7520684
2020 P-selectin (CD62P) and soluble TREM-like transcript-1 (sTLT-1) are associated with coronary artery disease: a case control study. BMC cardiovascular disorders 22 32831023
2003 Effect of tumor stage and nephrectomy on CD62P expression and sP-selectin concentration in renal cancer. Neoplasma 22 12937838
2016 The Synergistic Effect of Serine with Selenocompounds on the Expression of SelP and GPx in HepG2 Cells. Biological trace element research 21 26944060
2009 Variation in the upstream region of P-Selectin (SELP) is a risk factor for SLE. Genes and immunity 20 19404301
2009 Effects of banding or burdizzo castration of bulls on neutrophil phagocytosis and respiratory burst, CD62-L expression, and serum interleukin-8 concentration. Journal of animal science 20 19617515
2008 The effect of gestational age on platelet surface expression of CD62P in preterm newborns. Platelets 20 18432525
2020 ADP-Mediated Upregulation of Expression of CD62P on Human Platelets Is Critically Dependent on Co-Activation of P2Y1 and P2Y12 Receptors. Pharmaceuticals (Basel, Switzerland) 19 33255391
2014 Cellular adhesion gene SELP is associated with rheumatoid arthritis and displays differential allelic expression. PloS one 19 25147926
2000 Selectins (CD62L, CD62P) and megakaryocytic glycoproteins (CD41a, CD42b) mediate megakaryocyte-fibroblast interactions in human bone marrow. Leukemia research 19 11077115
2003 Equine platelet CD62P (P-selectin) expression: a phenotypic and morphologic study. Veterinary immunology and immunopathology 18 12543548
1996 A monoclonal antibody directed against a granule membrane glycoprotein (GMP-140/PADGEM, P-selectin, CD62P) inhibits ristocetin-induced platelet aggregation. British journal of haematology 18 8603015
2015 Changes of soluble CD40 ligand in the progression of acute myocardial infarction associate to endothelial nitric oxide synthase polymorphisms and vascular endothelial growth factor but not to platelet CD62P expression. Translational research : the journal of laboratory and clinical medicine 17 26279254
2009 SELP and SELPLG genetic variation is associated with cell surface measures of SELP and SELPLG: the Atherosclerosis Risk in Communities Carotid MRI Study. Clinical chemistry 17 19395438
2011 Platelet function alterations and their relation to P-selectin (CD62P) expression in children with iron deficiency anemia. Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis 16 21245745
2009 Capture of platelets to the endothelium of the femoral vein is mediated by CD62P and CD162. Platelets 16 19852690
2003 Platelet CD62p expression and microparticle in murine acquired immune deficiency syndrome and chronic ethanol consumption. Alcohol and alcoholism (Oxford, Oxfordshire) 16 12554603
2003 Characterization of platelet and soluble-porcine P-selectin (CD62P). Veterinary immunology and immunopathology 15 14592730
2001 Von Willebrand factor, a key protein in the exposure of CD62P on platelets. Biomaterials 15 11511037
1993 T cells bind to the endothelial adhesion molecule GMP-140 (P-selectin). Transplantation 15 7504345
1989 PADGEM protein in human erythroleukemia cells. Blood 15 2465041
2018 The Effects of Curcumae Longae Radix, Curcuma phaeocaulis Radix and Their Processed Products on Epo/EpoR Pathway and CD62p. Frontiers in pharmacology 14 30038572
2018 Efficacy of different hemodialysis methods on dendritic cell marker CD40 and CD80 and platelet activation marker CD62P and P10 in patients with chronic renal failure. Journal of clinical laboratory analysis 14 30499177
2005 SELPLG and SELP single-nucleotide polymorphisms in multiple sclerosis. Neuroscience letters 14 16257118

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