Affinage

PUM1

Pumilio homolog 1 · UniProt Q14671

Length
1186 aa
Mass
126.5 kDa
Annotated
2026-04-28
54 papers in source corpus 21 papers cited in narrative 23 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PUM1 is a sequence-specific RNA-binding protein that post-transcriptionally represses a broad regulon of mRNAs governing cell proliferation, innate immunity, germ cell development, and erythropoiesis. It recognizes a UGUAHAUA consensus (Pumilio Binding Element) in 3′ UTRs and represses target expression by accelerating mRNA deadenylation through recruitment of the CCR4-NOT complex—a process modulated by phosphorylation of PUM1's intrinsically disordered regions and dependent on PABPC1/PABPC4—and by inhibiting translation, as demonstrated for targets including CDKN1B/p27, TLR4, HBG1 (γ-globin), and KCNK3 (PMID:18411299, PMID:30811992, PMID:35034101, PMID:35667093). PUM1 cooperates with NANOS cofactors to repress specific targets such as FOXM1 and SIAH1 and assembles into RNP regulons distinct from those of PUM2, reflecting paralog-specific cofactor associations (PMID:30269240, PMID:35743036, PMID:32316190). Heterozygous loss-of-function mutations in PUM1 cause dosage-dependent neurodegenerative and neurodevelopmental disease by derepressing targets including ATXN1 (PMID:29474920).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 2008 High

    Establishing PUM1 as a sequence-specific mRNA decay factor: genome-wide target identification revealed that PUM1 binds a UGUAHAUA consensus in 3′ UTRs and promotes turnover of hundreds of mRNAs enriched for cell-cycle and transcription regulators, filling the gap between Drosophila Pumilio biology and the human ortholog's direct RNA targets.

    Evidence RIP-chip in human cells with PUM1 knockdown and mRNA stability measurements

    PMID:18411299

    Open questions at the time
    • Mechanism of mRNA decay acceleration (deadenylase vs. decapping) not resolved
    • Relative contribution of translational repression vs. decay not separated
  2. 2014 Low

    Identification of CDKN1B/p27 as a key proliferative target: PUM1 silencing elevated p27 protein and the p27-CDK2 inhibitory complex, linking PUM1 to cell-cycle control through translational repression of a specific CDK inhibitor.

    Evidence siRNA knockdown, western blot, co-immunoprecipitation of p27-CDK2 in pancreatic cancer cells

    PMID:31128486

    Open questions at the time
    • Single Co-IP without reciprocal validation
    • Direct RNA binding to CDKN1B 3′ UTR not demonstrated in this study
  3. 2015 Medium

    Demonstration of an essential role in preimplantation development: Pum1 gene-trap homozygous embryos failed to survive past the preimplantation stage, establishing that PUM1 is indispensable for early mammalian embryogenesis.

    Evidence Mouse gene trap with embryo genotyping and in vitro fertilization

    PMID:25896760

    Open questions at the time
    • Target mRNAs responsible for preimplantation lethality not identified
    • Whether phenotype reflects maternal or zygotic PUM1 requirement unclear
  4. 2017 High

    Placement of PUM1 as a negative regulator of innate immunity: double-knockdown epistasis showed PUM1 represses LGP2 mRNA, whose derepression triggers a biphasic cascade activating interferon and inflammatory genes, a function not shared by PUM2.

    Evidence siRNA double-knockdown of PUM1 and LGP2, RT-qPCR, HSV-1 functional assay in human cells

    PMID:28760986

    Open questions at the time
    • Direct PUM1 binding to LGP2 3′ UTR not confirmed by RIP in this study
    • Whether PUM1 represses LGP2 via decay, translational inhibition, or both not resolved
  5. 2018 High

    Linking PUM1 dosage to human neurological disease: patient mutations reducing PUM1 protein by ~25–50% caused proportional derepression of targets including ATXN1, establishing PUM1 haploinsufficiency as a cause of neurodegenerative (adult-onset ataxia) and neurodevelopmental (infantile) syndromes.

    Evidence Patient-derived lymphoblastoid cells, western blot, target mRNA/protein quantification across multiple mutation classes

    PMID:29474920

    Open questions at the time
    • Neuron-specific target regulon not fully defined
    • Whether ataxia phenotype can be rescued by ATXN1 reduction not tested
  6. 2018 Medium

    Revealing paralog-specific and NANOS-cooperative mechanisms: PUM1 represses SIAH1 in a PBE-independent manner distinct from PUM2, and NANOS3 cooperates with PUM1/PUM2 in 3′ UTR-mediated repression, demonstrating that cofactor identity determines target specificity.

    Evidence Luciferase reporter assays and EMSA with PUF domain constructs and NANOS paralogues

    PMID:30269240

    Open questions at the time
    • PBE-independent binding mode structurally uncharacterized
    • Whether NANOS-PUM1 complex is direct or bridged by RNA not resolved
  7. 2019 High

    Genetic proof that PUM1/PUM2 control body size through CDKN1B: Pum1/Pum2 double-null mice showed dosage-dependent organ and body size reduction rescued by Cdkn1b co-deletion, confirming p27 as the critical downstream effector of Pumilio-mediated proliferative control.

    Evidence Mouse knockout epistasis (Pum1−/− × Cdkn1b−/−), translational reporter assays with PBE mutations

    PMID:30811992

    Open questions at the time
    • Contribution of individual Pumilio paralogs to tissue-specific size control not separated
    • Translational vs. decay contribution to p27 regulation in vivo not dissected
  8. 2019 High

    Extending PUM1's regulon to non-coding RNA: PUM1 binds and destabilizes the lncRNA HOTAIR, reducing trophoblast invasion, showing that PUM1 post-transcriptional control extends beyond protein-coding mRNAs.

    Evidence RIP, RNA pull-down, mRNA stability assay, trophoblast invasion assays

    PMID:31862314

    Open questions at the time
    • How PUM1 binding to lncRNA differs structurally from mRNA binding unknown
    • Whether HOTAIR destabilization involves CCR4-NOT not tested
  9. 2020 High

    Connecting PUM1 to DNA damage tolerance: DNA-damaging agents reduce PUM1 protein, relieving repression of translesion synthesis factor mRNAs (PCNA, UBE2A), thereby activating TLS—a regulatory circuit integrating genotoxic stress with post-transcriptional control.

    Evidence Transcriptome-wide mRNA stability analysis plus RIP-seq, PUM1 KD/OE, cisplatin treatment

    PMID:32375027

    Open questions at the time
    • Mechanism of PUM1 protein downregulation by DNA damage not identified
    • Whether PUM1 regulation of TLS operates through deadenylation or translation not resolved
  10. 2020 Medium

    Distinguishing PUM1 and PUM2 regulons at the systems level: despite recognizing the same RNA motif, PUM1 and PUM2 associate with distinct protein cofactor sets and regulate partially non-overlapping mRNA pools in human germ cells.

    Evidence RIP-Seq, RNA-Seq, and mass spectrometry-based interactome profiling in TCam-2 cells

    PMID:32316190

    Open questions at the time
    • Identity of PUM1-specific cofactors driving target selectivity not fully validated
    • Whether distinct regulons reflect competition or compartmentalization unknown
  11. 2022 High

    Defining PUM1 as a post-transcriptional regulator of the hemoglobin switch: PUM1 binds γ-globin (HBG1) mRNA and reduces both its stability and translational efficiency, contributing to fetal-to-adult hemoglobin switching, with PUM1 knockdown raising HbF ~22%.

    Evidence RIP, mRNA stability and translational efficiency assays in human erythroid cells, patient PUM1 mutation analysis

    PMID:35667093

    Open questions at the time
    • Whether PUM1 is a tractable therapeutic target for HbF induction not established
    • Interaction with other HbF regulators (BCL11A, LRF) not explored
  12. 2022 High

    Demonstrating PUM1 suppression of TLR4–NF-κB inflammatory signaling: PUM1 binds TLR4 3′ UTR and represses its translation, protecting mesenchymal stem cells from senescence and inflammation.

    Evidence 3′ UTR binding assays, PUM1 OE/KD, NF-κB activity assay, in vivo osteoarthritis mouse model with lentiviral gene therapy

    PMID:35034101

    Open questions at the time
    • Whether TLR4 repression involves deadenylation or purely translational inhibition not determined
  13. 2024 Medium

    Elucidating the biochemical mechanism of repression: PUM1's intrinsically disordered regions make multivalent contacts with the CCR4-NOT deadenylase complex to drive deadenylation, with phosphorylation within these IDRs continuously tuning the rate; PABPC1/PABPC4 are required for PUM1-mediated mRNA decay and modulate repression in a concentration-dependent manner.

    Evidence Structural biology, in vitro reconstitution of deadenylation, phosphorylation mutagenesis (preprint)

    Open questions at the time
    • Preprint not yet peer-reviewed
    • In vivo relevance of graded phosphorylation tuning not demonstrated
    • Structural details of IDR–CCR4-NOT interface at atomic resolution not available
  14. 2025 Medium

    Establishing a hypoxia-driven PUM1–KCNK3 axis in pulmonary hypertension: HIF1α directly activates PUM1 transcription under hypoxia, and PUM1 in turn destabilizes KCNK3 mRNA in pulmonary artery smooth muscle cells, with in vivo PUM1 knockdown attenuating experimental pulmonary hypertension.

    Evidence ChIP for HIF1α on PUM1 promoter, RIP for PUM1–KCNK3 mRNA, AAV9 knockdown in rat PH model

    PMID:41135634

    Open questions at the time
    • Whether KCNK3 is the sole effector of PUM1 in pulmonary hypertension not tested
    • Generalizability beyond rat model unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: the structural basis of PUM1's PBE-independent repression of certain targets; how PUM1-specific cofactors are selected to generate paralog-distinct regulons; and whether the graded phosphorylation-dependent tuning of CCR4-NOT recruitment operates in physiological contexts such as neuronal homeostasis and erythropoiesis.
  • No structural model of full-length PUM1 with IDRs bound to CCR4-NOT
  • Neuron-specific target regulon and its contribution to ataxia pathogenesis incompletely mapped
  • Therapeutic potential of modulating PUM1 activity for HbF induction or neurodegeneration untested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 10 GO:0098772 molecular function regulator activity 6 GO:0140098 catalytic activity, acting on RNA 5
Localization
GO:0005634 nucleus 1 GO:0005829 cytosol 1
Pathway
R-HSA-8953854 Metabolism of RNA 8 R-HSA-1640170 Cell Cycle 4 R-HSA-392499 Metabolism of proteins 4 R-HSA-168256 Immune System 2
Partners
CNOT1NANOS3PABPC1PABPC4PUM2
Complex memberships
PUM1-CCR4-NOT complexPUM1-NANOS3 complex

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2008 Human PUM1 binds a core consensus sequence UGUAHAUA in the 3' UTRs of associated mRNAs; PUM1 knockdown demonstrated it enhances decay of associated mRNAs; PUM1 relocalizes to stress granules, consistent with a role in translational repression. Associated mRNAs are enriched for transcriptional regulators and cell cycle/proliferation genes. Ribonomic analysis (RIP-chip), genome-wide mRNA target identification, PUM1 knockdown, stress granule localization by imaging Molecular and cellular biology High 18411299
2018 Missense mutations in PUM1 reduce PUM1 protein levels (~25% in adult-onset, ~50% in infantile-onset cases), causing proportional upregulation of known PUM1 target mRNAs (including ATXN1), linking PUM1 haploinsufficiency to neurodegenerative and neurodevelopmental phenotypes. PUM1 functions as a post-transcriptional repressor whose dosage directly controls downstream target protein levels. Patient-derived cell studies, western blot, target mRNA/protein quantification in cells from individuals with PUM1 deletions or de novo missense variants Cell High 29474920
2019 PUM1 and PUM2 repress translation of CDKN1B (p27) by binding Pumilio binding elements (PBEs) in the CDKN1B 3' UTR, promoting G1-S transition and cell proliferation. Pum1/Pum2 double-null mice show gene dosage-dependent body/organ size reductions rescued by Cdkn1b deficiency. PUM1 and PUM2 also engage in auto-regulatory and reciprocal post-transcriptional repression of each other. Mouse knockout genetics, translational reporter assays, epistasis (Pum1-/- x Cdkn1b-/- rescue), 3'UTR PBE binding assays Cell reports High 30811992
2022 PUM1 binds to the 3' UTR of TLR4 mRNA and suppresses TLR4 mRNA translation, thereby regulating NF-κB activity in human mesenchymal stem cells. PUM1 overexpression protects MSCs against H2O2-induced senescence and inflammation by suppressing TLR4-mediated NF-κB signaling. 3'UTR binding assays, PUM1 overexpression/knockdown, western blot, NF-κB activity assay, in vivo OA mouse model with lentiviral PUM1 gene therapy Cell death and differentiation High 35034101
2017 PUM1 acts as a biphasic negative regulator of innate immunity genes by repressing LGP2 expression. PUM1 knockdown triggers an initial upregulation of LGP2, CXCL10, IL6, and PKR (phase 1), followed by upregulation of RIG-I, MDA5, IFNβ, and other innate immunity genes (phase 2) downstream of LGP2. Simultaneous depletion of PUM1 and LGP2 abolished phase 1 and 2 gene upregulation. PUM2 depletion did not replicate this effect. siRNA knockdown of PUM1 and LGP2 (single and double), RT-qPCR, IFNβ functional assay (HSV-1 replication suppression) Proceedings of the National Academy of Sciences of the United States of America High 28760986
2019 PUM1 binding to the 3' UTR of HOTAIR lncRNA decreases its half-life and steady-state level, thereby inhibiting trophoblast invasion. RNA-protein pull-down and mRNA stability assays confirmed PUM1 as a direct binding partner of HOTAIR mRNA. PUM1 overexpression impairs trophoblast invasion, while PUM1 knockdown enhances it, through HOTAIR upregulation. RIP, RNA pull-down, mRNA stability assay, trophoblast invasion assays, lncRNA transcriptome sequencing, villous explant culture model Molecular therapy : the journal of the American Society of Gene Therapy High 31862314
2020 PUM1 promotes degradation of 48 specific target mRNAs. DNA-damaging agents (e.g., cisplatin) reduce PUM1 protein abundance, thereby relieving PUM1-mediated decay of PCNA and UBE2A mRNAs encoding translesion synthesis (TLS) factors, which activates TLS. PUM1 overexpression impairs TLS and increases cisplatin sensitivity. Transcriptome-wide mRNA stability analysis combined with PUM1 RIP-seq, PUM1 knockdown/overexpression, RNA-seq, cisplatin treatment Cell reports High 32375027
2022 PUM1 directly binds γ-globin (HBG1) mRNA, reducing its stability and translational efficiency, which contributes to the fetal-to-adult hemoglobin switch. PUM1 expression is regulated by erythroid master transcription factor EKLF/KLF1. PUM1 knockdown in human erythroid cells leads to ~22% HbF without affecting β-globin levels. RIP, mRNA stability assay, translational efficiency measurement, PUM1 knockdown in human erythroid cells, patient HbF measurement (PUM1 RNA-binding domain mutation) Blood advances High 35667093
2018 PUM1 and PUM2 exhibit mechanistically distinct modes of regulation of SIAH1 mRNA: PUM1 (unlike PUM2) exerts PBE-independent repression of SIAH1 3'UTR-dependent expression. The PUF domains of PUM1 and PUM2 show different EMSA complex formation patterns with SIAH1 3'UTRs. NANOS3 (but not NANOS2) directly binds SIAH1 3'UTR independently of PBEs or the PUF domain and cooperates with PUM1/PUM2 in repression. Luciferase reporter assays, EMSA, cooperativity assays with NANOS paralogues, NANOS patient mutation constructs Cellular and molecular life sciences : CMLS Medium 30269240
2021 PUM1 binds the 3'UTR of LRP6 mRNA (verified by RNA pull-down, RIP, luciferase reporter), reducing LRP6 mRNA and protein levels, and thereby inhibiting trophoblast cell proliferation, migration, and invasion. PUM1 depletion-mediated promotion of trophoblast function was reversed by LRP6 knockdown. RNA pull-down, RNA immunoprecipitation, luciferase reporter assay, RT-qPCR, western blot, functional cell assays Biochemistry and cell biology = Biochimie et biologie cellulaire Medium 34734756
2019 PUM1 knockdown in pancreatic cancer cells activates the PERK/eIF2/ATF4 signaling pathway (increased p-PERK, p-EIF2A, ATF4), suppressing proliferation, migration, invasion, and EMT. A PERK inhibitor reversed these effects, establishing a negative regulatory relationship between PUM1 and this stress-response pathway. PUM1 levels negatively correlate with p-PERK levels in PDAC tissues. cDNA microarrays, siRNA knockdown, western blot, in vitro and in vivo functional assays, PERK inhibitor rescue Cell death & disease Medium 31395860
2022 NANOS3 in complex with PUM1 causes 3'UTR-mediated repression of FOXM1 mRNA, a transcription factor critical for G2/M phase transition, thereby controlling G2/M progression in a human primordial germ cell model (TCam-2 cells). FOXM1 potentially acts as a transcriptional activator of NANOS3 and PUM1, suggesting a regulatory feedback loop. RNA-sequencing, 3'UTR luciferase reporter assay, NANOS3 and PUM1 overexpression in TCam-2 cells, cell cycle analysis International journal of molecular sciences Medium 35743036
2020 PUM1 forms distinct RNP regulatory networks (RNA regulons) compared to PUM2, associating with different sets of protein cofactors involved in RNA processing, despite recognizing the same RNA binding motif. PUM1 and PUM2 regulate partially non-overlapping pools of mRNAs in human male germ cells. RIP-Seq, RNA-Seq, global mass spectrometry-based protein interactome profiling (TCam-2 human male germ cell line) Cells Medium 32316190
2021 PUM1 represses CDKN1B (p27) protein expression through translational control (not mRNA level) in prostate cancer cells. PUM1 knockdown consistently elevated CDKN1B protein via increased translation but did not affect CDKN1B mRNA levels; PUM1 overexpression reduced CDKN1B protein. PUM1 knockdown/overexpression, western blot, polysome/translation assays, subcutaneous tumor xenograft Journal of biomedical research Medium 34531333
2023 PUM1 binds directly to the DEPTOR mRNA pumilio response element (PRE), maintaining DEPTOR transcript stability and preventing its degradation. PUM1-mediated DEPTOR upregulation inhibits mTORC1 and relieves inhibitory feedback to PI3K, activating PI3K-Akt signaling and promoting glycolysis in gastric cancer cells. RNA-sequencing, bioinformatics, metabolomics, RNA immunoprecipitation, luciferase reporter assay, PUM1 knockdown/overexpression, in vivo xenograft Advanced science (Weinheim, Baden-Wurttemberg, Germany) Medium 37469018
2014 PUM1 silencing increased p27 expression and the amount of the p27-CDK2 complex in pancreatic cancer cells, consistent with PUM1-mediated translational repression of p27 (CDKN1B). PUM1 levels correlate inversely with p27 activity. PUM1 siRNA knockdown, western blot, immunoprecipitation of p27-CDK2 complex, cell proliferation assays Phytomedicine : international journal of phytotherapy and phytopharmacology Low 31128486
2025 PUM1 binds KCNK3 mRNA and destabilizes it in pulmonary artery smooth muscle cells (PASMCs). HIF1α, activated by hypoxia, directly binds the PUM1 promoter to transcriptionally upregulate PUM1 expression, establishing a HIF1α-PUM1-KCNK3 regulatory axis driving pathological PASMC phenotypes. AAV9-mediated PUM1 knockdown attenuated pulmonary hypertension in vivo. ChIP (HIF1α binding to PUM1 promoter), RNA immunoprecipitation (PUM1-KCNK3 mRNA), mRNA stability assay, AAV9 knockdown in vivo rat PH models, PASMC functional assays Biochimica et biophysica acta. Molecular basis of disease Medium 41135634
2024 PUM1's intrinsically disordered regions (IDRs) interact multivalently with distinct binding sites on the CCR4-NOT deadenylase complex to recruit it to target mRNAs and promote deadenylation. Phosphorylation within these IDRs tunes the deadenylation rate in a continuously graded manner. PUM1-mediated repression requires CCR4-NOT deadenylase activity and PABPC1/PABPC4, which stabilize poly(A) tails and modulate PUM1 repression in a concentration-dependent manner. Structural biology, biochemical reconstitution, in vitro deadenylation assays, phosphorylation mutagenesis, IDR binding mapping bioRxivpreprint Medium
2025 PUM1-mediated mRNA repression requires CCR4-NOT deadenylase and is dependent on PABPC1 and PABPC4; PUM1 associates with PABPCs, and in their absence, targets escape PUM1 control. PABPC abundance inversely tunes PUM repression in a concentration-dependent manner. Co-immunoprecipitation (PUM-PABPC association), mRNA decay assays, genetic depletion of deadenylase components and PABPCs, reporter assays bioRxivpreprint Medium
2025 PUM1 negatively regulates innate immunity gene expression both at steady state and during SARS-CoV-2 infection. PUM1 depletion slightly increases intracellular SARS-CoV-2 RNA levels, suggesting mild antiviral or indirect host-factor regulatory activity, but does not affect progeny virion production. PUM1 depletion (siRNA/shRNA), intracellular viral RNA quantification, innate immunity gene expression analysis, PRE binding analysis The Journal of general virology Medium 40956600
2021 PUM1 interacts with DDX5 mRNA 3' UTR and positively regulates DDX5 mRNA expression in cetuximab-resistant colon cancer cells. PUM1 knockout reduced DDX5 levels and decreased cell proliferation in resistant lines. CRISPR-Cas9 PUM1 and DDX5 knockout, qPCR, immunoblot, cell viability assays Frontiers in cell and developmental biology Low 34447749
2023 PUM1 binds and stabilizes PAK6 mRNA in lung adenocarcinoma cells, as confirmed by RNA immunoprecipitation and luciferase assays. PUM1 silencing reduced PAK6 levels, promoted ferroptosis (elevated Fe2+ and MDA), and reduced tumor growth in vivo, effects reversed by PAK6 restoration. RNA immunoprecipitation, luciferase assay, PUM1 knockdown, ferroptosis markers (Fe2+, MDA), in vivo xenograft Pathology, research and practice Low 40694989
2015 A gene trap mutation in the mouse Pum1 gene (Pum1XE002) results in loss of preimplantation embryos; no homozygous blastocysts are recovered, indicating an essential role for PUM1 in very early embryonic development or fertilization. Mouse gene trap, embryo genotyping, in vitro fertilization, embryo culture Biochemical and biophysical research communications Medium 25896760

Source papers

Stage 0 corpus · 54 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2008 Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets. Molecular and cellular biology 131 18411299
2018 A Mild PUM1 Mutation Is Associated with Adult-Onset Ataxia, whereas Haploinsufficiency Causes Developmental Delay and Seizures. Cell 109 29474920
2002 Cloning and comparative sequence analysis of PUM1 and PUM2 genes, human members of the Pumilio family of RNA-binding proteins. Gene 77 12459267
2022 TLR4 downregulation by the RNA-binding protein PUM1 alleviates cellular aging and osteoarthritis. Cell death and differentiation 70 35034101
2020 Circ_PUM1 promotes the development of endometrial cancer by targeting the miR-136/NOTCH3 pathway. Journal of cellular and molecular medicine 55 32073729
2003 Mouse Pum1 and Pum2 genes, members of the Pumilio family of RNA-binding proteins, show differential expression in fetal and adult hematopoietic stem cells and progenitors. Blood cells, molecules & diseases 54 12667987
2019 Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell reports 46 30811992
2019 PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells. Cell death & disease 41 31395860
2018 PUM1 promotes ovarian cancer proliferation, migration and invasion. Biochemical and biophysical research communications 38 29428722
2019 Upregulation of PUM1 Expression in Preeclampsia Impairs Trophoblast Invasion by Negatively Regulating the Expression of the lncRNA HOTAIR. Molecular therapy : the journal of the American Society of Gene Therapy 37 31862314
2017 PUM1 is a biphasic negative regulator of innate immunity genes by suppressing LGP2. Proceedings of the National Academy of Sciences of the United States of America 33 28760986
2023 PUM1 Promotes Tumor Progression by Activating DEPTOR-Meditated Glycolysis in Gastric Cancer. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 28 37469018
2018 MiR-411-5p acts as a tumor suppressor in non-small cell lung cancer through targeting PUM1. European review for medical and pharmacological sciences 28 30229827
2019 Triptolide enhances TRAIL sensitivity of pancreatic cancer cells by activating autophagy via downregulation of PUM1. Phytomedicine : international journal of phytotherapy and phytopharmacology 25 31128486
2021 Long non-coding RNA NORAD inhibition upregulates microRNA-323a-3p to suppress tumorigenesis and development of breast cancer through the PUM1/eIF2 axis. Cell cycle (Georgetown, Tex.) 23 34125645
2021 RNA binding protein PUM1 promotes colon cancer cell proliferation and migration. International journal of biological macromolecules 22 33508364
2021 Circular RNA PUM1 (CircPUM1) attenuates trophoblast cell dysfunction and inflammation in recurrent spontaneous abortion via the MicroRNA-30a-5p (miR-30a-5p)/JUNB axis. Bioengineered 21 34519628
2020 Systematic Analysis of Targets of Pumilio-Mediated mRNA Decay Reveals that PUM1 Repression by DNA Damage Activates Translesion Synthesis. Cell reports 19 32375027
2020 Novel three-way complex rearrangement of TRPM1-PUM1-LCK in a case of agminated Spitz nevi arising in a giant congenital hyperpigmented macule. Pigment cell & melanoma research 19 32386465
2020 Gene-gene and gene-lifestyle interactions of AKAP11, KCNMA1, PUM1, SPTBN1, and EPDR1 on osteoporosis risk in middle-aged adults. Nutrition (Burbank, Los Angeles County, Calif.) 19 32619791
2010 A structural-based statistical approach suggests a cooperative activity of PUM1 and miR-410 in human 3'-untranslated regions. Silence 19 20860814
2022 Morin inhibits colon cancer stem cells by inhibiting PUM1 expression in vitro. Medical oncology (Northwood, London, England) 16 36224472
2018 PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cellular and molecular life sciences : CMLS 15 30269240
2016 Morphotype-specific effector functions of Cryptococcus neoformans PUM1. Scientific reports 14 27008977
2022 PUM1 mediates the posttranscriptional regulation of human fetal hemoglobin. Blood advances 13 35667093
2021 PUM1 Is Overexpressed in Colon Cancer Cells With Acquired Resistance to Cetuximab. Frontiers in cell and developmental biology 12 34447749
2021 Silencing of circular RNA PUM1 inhibits clear cell renal cell carcinoma progression through the miR-340-5p/FABP7 axis. Journal of receptor and signal transduction research 11 33472512
2020 Characterization of RNP Networks of PUM1 and PUM2 Post-Transcriptional Regulators in TCam-2 Cells, a Human Male Germ Cell Model. Cells 11 32316190
2015 Loss of preimplantation embryo resulting from a Pum1 gene trap mutation. Biochemical and biophysical research communications 11 25896760
2023 Inducing Cytotoxicity in Colon Cancer Cells and Suppressing Cancer Stem Cells by Dolasetron and Ketoprofen through Inhibition of RNA Binding Protein PUM1. Toxics 10 37624174
2022 Circular RNA PUM1 performs as a competing endogenous RNA of microRNA-340-5p to mediate DEAD-box helicase 5 to mitigate cerebral ischemia-reperfusion injury. Bioengineered 10 35510394
2022 Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model. International journal of molecular sciences 10 35743036
2021 PUM1 represses CDKN1B translation and contributes to prostate cancer progression. Journal of biomedical research 10 34531333
2023 Exosome-mediated circGMPS facilitates the development of gastric cancer cells through miR-144-3p/PUM1. Cytotechnology 7 38304630
2019 Investigating PUM1 mutations in a Taiwanese cohort with cerebellar ataxia. Parkinsonism & related disorders 7 31422002
2021 PUM1 and RNase P genes as potential cell-free DNA markers in breast cancer. Journal of clinical laboratory analysis 6 33522650
2023 PUF-8, a C. elegans ortholog of the RNA-binding proteins PUM1 and PUM2, is required for robustness of the cell death fate. Development (Cambridge, England) 5 37747106
2011 Spatial-temporal expression of pum1 and pum2 in medaka Oryzias latipes. Journal of fish biology 5 22220892
2023 Up-regulation of PUM1 by miR-218-5p promotes colorectal tumor-initiating cell properties and tumorigenesis by regulating the PI3K/AKT axis. Journal of gastrointestinal oncology 4 36915463
2021 PUM1 modulates trophoblast cell proliferation and migration through LRP6. Biochemistry and cell biology = Biochimie et biologie cellulaire 4 34734756
2024 PUM1-TRAF3 fusion protein activates non-canonical NF-κB signaling via rescued NIK in biliary tract cancer. NPJ precision oncology 3 39090283
2018 Variation analysis of PUM1 gene in Chinese women with primary ovarian insufficiency. Journal of assisted reproduction and genetics 3 29297114
2024 PUM1 and PGK1 are Favorable Housekeeping Genes over Established Biodosimetry-related Housekeeping Genes such as HPRT1, ITFG1, DPM1, MRPS5, 18S rRNA and Others after Radiation Exposure. Radiation research 2 38471523
2022 A de novo PUM1 Variant in a Girl With a Dravet-Like Syndrome: Case Report and Literature Review. Frontiers in pediatrics 2 35386260
2025 A new developmental and epileptic encephalopathy: PUM1-neurodevelopmental disorder with epilepsy with myoclonic-atonic seizures. Seizure 1 40472467
2025 PUM2 binds SARS-CoV-2 RNA and PUM1 mildly reduces viral RNA levels, but neither protein affects progeny virus production. The Journal of general virology 1 40956600
2025 The HIF1α-PUM1-KCNK3 axis drives pulmonary hypertension by destabilizing KCNK3 mRNA in pulmonary artery smooth muscle cells. Biochimica et biophysica acta. Molecular basis of disease 1 41135634
2023 Exploration of the effect of PUM1/Cripto-1 pathway on ferroptosis by regulating macrophage polarization in allogeneic blood transfused mice. Aging 1 37387538
2026 Evaluation of the circular RNA Pum1_0014, miRNA-146a, and miRNA-141-3p as biomarkers in PCOS. Journal of Taibah University Medical Sciences 0 41908224
2026 Fusobacterium nucleatum drives colorectal cancer progression through the circPTBP3/miR-760/PUM1 axis. Oncogene 0 41912775
2025 PUM2 binds SARS-CoV-2 RNA and PUM1 mildly reduces viral RNA levels, but neither protein affects progeny virus production. bioRxiv : the preprint server for biology 0 40666984
2025 PUM1 enhances PAK6 mRNA stability and contributes to growth and ferroptosis resistance in lung adenocarcinoma cells. Pathology, research and practice 0 40694989
2025 Retraction Note: MiR-411-5p acts as a tumor suppressor in non-small cell lung cancer through targeting PUM1. European review for medical and pharmacological sciences 0 41342067
2020 Repression of PUM1-mediated mRNA decay activates translesion synthesis after DNA damage. Molecular & cellular oncology 0 33241107