Affinage

PUM2

Pumilio homolog 2 · UniProt Q8TB72

Length
1066 aa
Mass
114.2 kDa
Annotated
2026-04-28
52 papers in source corpus 29 papers cited in narrative 29 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PUM2 is a PUF-family sequence-specific RNA-binding protein that recognizes Pumilio-binding elements (PBEs/PREs; core motif UGUANAUA) in 3′UTRs of target mRNAs and represses their expression through translational inhibition and mRNA destabilization, thereby governing diverse processes including mitochondrial dynamics, cell cycle progression, body size control, stem cell differentiation, and neuronal circuit wiring. PUM2 represses targets such as Mff, Cdkn1b/p27, Sirt1, JAK2, RUNX2, DLX5, FOXO3, and NRF2 by recruiting the CCR4-NOT deadenylase complex in a PABPC1/PABPC4-dependent manner, while its target selectivity is modulated by cofactors including DAZL and BOULE and by the lncRNA NORAD, which sequesters PUM2 away from its mRNA targets [PMID:11780640, PMID:15607425, PMID:30642763, PMID:30811992, PMID:39832611, PMID:bio_10.1101_2025.10.02.680050]. PUM2 protein stability is regulated by SUMOylation (which promotes its degradation) and SENP1-mediated deSUMOylation (which stabilizes it), and PUM2 has a non-canonical mitotic role at centrosomes where it binds the D-box of Aurora-A kinase, protecting it from APC/C(Cdh1)-dependent ubiquitination and enhancing its kinase activity (PMID:32997416, PMID:39794619, PMID:21589936). In neurons, PUM2 localizes to the soma and retains PBE-containing mRNAs in the cell body, controlling axonal transcriptome composition and thereby regulating axon growth, branching, and cortical cytoarchitecture (PMID:31606248, PMID:35262486).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2001 High

    The fundamental RNA-recognition specificity of PUM2 was established: PUM2's C-terminal PUM-HD binds a defined PBE consensus (UGUANAUA) with nanomolar affinity, defining it as a sequence-specific post-transcriptional regulator.

    Evidence SELEX with recombinant PUM2 PUM-HD and quantitative binding (Kd ~6.5 nM)

    PMID:11780640

    Open questions at the time
    • No endogenous mRNA targets were identified at this stage
    • No mechanism of repression (translational vs. decay) was determined
  2. 2005 High

    PUM2 was shown to bind biologically relevant RNA targets (NRE, SDAD1 3′UTR) and to operate with distinct protein cofactors (DAZL, BOULE) that determine target selectivity, establishing PUM2 as a combinatorial RNP regulator rather than a standalone factor.

    Evidence Co-immunoprecipitation, RNA binding assays with mutagenesis, and domain mapping in human cell extracts

    PMID:15607425 PMID:15806553

    Open questions at the time
    • How cofactors alter RNA target selection was not mechanistically resolved
    • In vivo significance of PUM2–DAZL and PUM2–BOULE complexes was not tested
  3. 2011 High

    A non-canonical, RNA-independent role was discovered: PUM2 physically associates with Aurora-A kinase at centrosomes during mitosis, protects it from APC/C(Cdh1)-mediated degradation, and enhances its kinase activity, revealing a moonlighting function beyond translational repression.

    Evidence Co-IP, ubiquitination assays, kinase activity assays, and immunofluorescence localization in human cells

    PMID:21589936

    Open questions at the time
    • Whether PUM2's RNA-binding and Aurora-A stabilization functions are coordinated or independent was not resolved
    • Structural basis of PUM2–D-box interaction was not determined
  4. 2019 High

    PUM2 was established as a key post-transcriptional repressor controlling mitochondrial dynamics (via Mff), cell cycle/body size (via Cdkn1b), mesenchymal stem cell fate (via JAK2/RUNX2), and neuronal architecture (via somatic mRNA retention), demonstrating broad physiological relevance across tissues and organisms.

    Evidence CRISPR KO mice (Mff target), genetic null mouse models with epistasis (Cdkn1b), 3′UTR reporters and zebrafish knockdown (JAK2/RUNX2), shRNA knockdown with mRNA localization imaging and in vivo axon analysis (neuronal soma retention)

    PMID:30642763 PMID:30811992 PMID:31595981 PMID:31606248

    Open questions at the time
    • The deadenylase/decay machinery recruited by PUM2 was not yet identified
    • How PUM2 physically retains mRNAs in the soma vs. repressing translation was not mechanistically distinguished
  5. 2019 High

    Cooperativity in PUM2 binding to multi-PBE targets was shown to be RNA-structure-mediated rather than protein–protein-mediated, revealing that conformational redistribution of RNA upon PUM2 binding modulates access to additional sites.

    Evidence Quantitative equilibrium binding across 68 synthetic RNA constructs with statistical mechanical modeling

    PMID:30914482

    Open questions at the time
    • Whether RNA-structure-mediated cooperativity operates on endogenous mRNAs in vivo was not tested
    • Effect on translational repression efficiency of cooperative vs. non-cooperative binding was not measured
  6. 2020 Medium

    Post-translational regulation of PUM2 by SUMOylation was identified: SUMO2/3 conjugation reduces PUM2 stability, diminishing its repressive capacity on targets such as CEBPD, linking SUMO signaling to PUM2-dependent gene regulation.

    Evidence Co-IP for SUMOylation, RIP for RNA binding, luciferase reporters in glioma cells

    PMID:32997416

    Open questions at the time
    • The E3 SUMO ligase responsible was not identified
    • Whether SUMOylation directly alters RNA binding or only protein stability was not distinguished
  7. 2020 Medium

    PUM1 and PUM2 were shown to form distinct RNP complexes with partially non-overlapping interactomes and target mRNAs in human male germ cells, establishing functional divergence between the two paralogs.

    Evidence RIP-Seq and mass spectrometry interactome profiling in TCam-2 cells

    PMID:32316190

    Open questions at the time
    • Functional consequences of paralog-specific targets were not tested
    • Whether divergence is conserved across tissues was not assessed
  8. 2022 High

    PUM2's role in cortical development was refined: it directly binds and translationally represses Sox5, Bcl11b/Ctip2, and Rorβ mRNAs in neocortical neurons, shaping area-specific cytoarchitecture and subcerebral projection neuron identity in vivo.

    Evidence Conditional Pum2 KO (Emx1-Cre), single-molecule FISH, retrograde tracing, in utero electroporation in mouse cortex

    PMID:35262486

    Open questions at the time
    • Whether PUM2 controls these targets via deadenylation or translational initiation block was not determined
    • Interaction with other RNA-binding proteins in cortical progenitors was not mapped
  9. 2025 Medium

    SENP1-mediated deSUMOylation was identified as the counterbalancing mechanism that stabilizes PUM2, and the downstream functional consequence was mapped: stabilized PUM2 represses NRF2 mRNA, reducing glycolytic gene expression in astrocytes.

    Evidence Ni²⁺-NTA pull-down and co-IP for deSUMOylation, 3′UTR binding assay, APP/PS1 transgenic mouse model

    PMID:39794619

    Open questions at the time
    • Whether SENP1-PUM2 axis operates globally or only in astrocytes is unknown
    • Quantitative relationship between SUMOylation state and PUM2 activity is not established
  10. 2025 Medium

    The effector mechanism of PUM2-mediated repression was identified: PUM2 recruits the CCR4-NOT deadenylase complex, and this repression requires and is antagonized by PABPC1/PABPC4 concentration, establishing PUM2 as a deadenylation-dependent repressor.

    Evidence (preprint) mRNA stability assays, co-IP of PUM2 with PABPCs, deadenylase requirement assays, PABPC titration

    PMID:bio_10.1101_2025.10.02.680050

    Open questions at the time
    • Peer review pending
    • Whether all PUM2 targets are repressed via deadenylation or some use a deadenylation-independent mechanism was not resolved
    • Direct PUM2–CNOT subunit interaction was not mapped
  11. 2025 Medium

    The lncRNA NORAD was shown to act as a molecular sponge for PUM2, and ischemia-reperfusion shifts the NORAD–PUM2 equilibrium to de-repress Mff, causing mitochondrial fragmentation and neuronal injury — establishing NORAD as a physiological rheostat of PUM2 activity.

    Evidence RNA-protein binding assays, OGD/R cell model, MCAO/R mouse model with PUM2 viral overexpression

    PMID:39832611

    Open questions at the time
    • Whether NORAD regulation of PUM2 is a general mechanism across tissues or restricted to ischemic neurons is not resolved
    • Stoichiometry of NORAD–PUM2 interaction in physiological conditions is unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Major unresolved questions include the structural basis of PUM2-mediated CCR4-NOT recruitment, the mechanism by which PUM2 retains mRNAs in the neuronal soma versus canonical translational repression, and whether SUMOylation/deSUMOylation regulates PUM2 activity genome-wide or in a target-specific manner.
  • No cryo-EM or structural model of PUM2 in complex with CCR4-NOT
  • Mechanism of somatic mRNA retention versus translational silencing not distinguished
  • Genome-wide impact of SUMO cycling on PUM2 target repertoire unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 8 GO:0140110 transcription regulator activity 6 GO:0140098 catalytic activity, acting on RNA 4 GO:0098772 molecular function regulator activity 2
Localization
GO:0005829 cytosol 2 GO:0005815 microtubule organizing center 1
Pathway
R-HSA-8953854 Metabolism of RNA 7 R-HSA-392499 Metabolism of proteins 4 R-HSA-112316 Neuronal System 3 R-HSA-1266738 Developmental Biology 2 R-HSA-1640170 Cell Cycle 2 R-HSA-5357801 Programmed Cell Death 2
Partners
AURKABOLLDAZLNANOS3PABPC1PABPC4SENP1

Evidence

Reading pass · 29 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 PUM2 contains a C-terminal PUM-HD RNA-binding domain and binds a consensus RNA sequence (PBE: UGUANAUARNNNNBBBBSCCS) with high affinity (Kd ~6.5 nM), as determined by SELEX with recombinant PUM2 PUM-HD protein. SELEX (iterative amplification-selection), recombinant protein binding assay RNA High 11780640
2005 Human PUM2 specifically binds the Drosophila Nanos Response Element (NRE); single nucleotide changes abolish binding. PUM2 and DAZL co-immunoprecipitate and can bind the same RNA targets, including the 3'UTR of human SDAD1 mRNA. Co-immunoprecipitation, RNA binding assays, mutational analysis of NRE Genomics High 15607425
2005 Human BOULE (BOL) forms homodimers and interacts with PUM2. BOL and PUM2 form a complex on a subset of PUM2 RNA targets distinct from targets bound by PUM2/DAZL, indicating that protein cofactors determine which RNA targets are bound by PUM2. Co-immunoprecipitation, domain mapping, RNA binding assays Molecular reproduction and development Medium 15806553
2005 PUM2 binds the 3'UTR of P2P-R mRNA via PUM2-binding elements (one perfect and two near-perfect PBEs), as confirmed by PUM2 pull-down combined with RT-PCR, suggesting PUM2-mediated translational regulation of P2P-R. RNA pull-down, RT-PCR Journal of cellular physiology Medium 15617101
2011 PUM2 physically binds the D-box of Aurora-A kinase, protects Aurora-A from APC/C(Cdh1)-mediated ubiquitination and proteasomal degradation, and enhances Aurora-A kinase activity. In interphase, PUM2 is cytoplasmic and acts as a translational repressor; in mitosis, PUM2 translocates to centrosomes to stabilize and activate Aurora-A. Co-immunoprecipitation, ubiquitination assay, kinase activity assay, overexpression/depletion, immunofluorescence localization PloS one High 21589936
2019 PUM2 inhibits translation of Mff (mitochondrial fission factor) mRNA, impairing mitochondrial fission and mitophagy. PUM2 levels increase with aging; CRISPR/Cas9-mediated Pum2 knockout in elderly mouse muscles enhances mitochondrial fission and mitophagy, improving mitochondrial quality control. Multi-omics, translational repression assays, CRISPR/Cas9 knockout in mice, cross-species (C. elegans puf-8 knockdown) Molecular cell High 30642763
2019 PUM1 and PUM2 repress translation of Cdkn1b (p27) by binding PBEs in its 3'UTR, promoting G1-S transition and cell proliferation, thereby controlling mouse body and organ size. Auto-regulatory and reciprocal post-transcriptional repression between Pum1 and Pum2 contributes to tissue-specific size control. Reporter assays (3'UTR luciferase), genetic null mouse models, epistasis (Cdkn1b deficiency rescue), cell proliferation assays Cell reports High 30811992
2019 Pum2 is restricted to the soma of developing neurons and retains PBE-containing mRNAs in the cell body, preventing their transport into axons. Pum2 knockdown causes PBE-containing mRNAs to appear and be translated in axons, and Pum2-deficient neurons exhibit axon growth, branching, and regeneration defects. Knockdown (shRNA), live-cell imaging, mRNA localization assays, in vivo axon branching analysis, axon regeneration assay Neuron High 31606248
2019 PUM2 directly binds the 3'UTR of JAK2 and RUNX2 mRNAs and represses their translation. PUM2 depletion blocks MSC adipogenesis and enhances osteogenesis; Pum2 CRISPR knockdown in zebrafish inhibits lipid accumulation and induces bone formation. 3'UTR reporter assays, RNA immunoprecipitation, CRISPR/Cas9 knockdown in zebrafish, differentiation assays Journal of cellular physiology High 31595981
2019 PUM2 cooperativity in binding multiple PBE sites is mediated by RNA structure rather than direct protein-protein interactions; binding of one PUM2 molecule redistributes RNA conformational states to modulate access to additional PBE sites (positive or negative cooperativity depending on structural stability). Equilibrium binding measurements on 68 synthetic RNAs, statistical mechanical modeling, two-temperature analysis RNA High 30914482
2018 PUM2 (but not PUM1) requires PBEs for repression of SIAH1 3'UTR-dependent reporter expression, whereas PUM1 exhibits PBE-independent repression. NANOS3 directly binds SIAH1 3'UTR independently of PBEs or the PUF domain, cooperating with PUM proteins in mRNA regulation. Luciferase reporter assays, EMSA, mutational analysis of PBEs Cellular and molecular life sciences Medium 30269240
2019 PUM2 competitively binds to STARD13 3'UTR with miR-590-3p and miR-9, as shown by RIP-seq and luciferase reporter assays. PUM2 overexpression inhibits osteosarcoma cell proliferation, migration, and stemness via this mechanism. RNA immunoprecipitation combined with RNA sequencing, luciferase reporter assay, cell functional assays Cell proliferation Medium 30084199
2020 SUMOylation of PUM2 by UBE2I/SUMO2/3 decreases PUM2 protein stability and reduces PUM2's inhibitory effect on CEBPD mRNA, leading to upregulation of CEBPD and downstream DSG2, which promotes glioma vasculogenic mimicry. Co-immunoprecipitation, immunofluorescence, RIP assay, ChIP assay, luciferase assay, cell invasion/migration assays Clinical and translational medicine Medium 32997416
2020 Pum2 mediates decay of Sirt1 mRNA via binding to two PBEs in the Sirt1 3'UTR. Pum2-mediated Sirt1 mRNA decay promotes LKB1 acetylation and represses AMPK pathway activity, contributing to cardiomyocyte apoptosis in hypoxia/reoxygenation injury. 3'UTR binding assay, overexpression/knockdown, Western blot for AMPK pathway, apoptosis assays Experimental cell research Medium 32437714
2020 Pum2 overexpression reduces Mff protein levels (without affecting mRNA) and preserves mitochondrial homeostasis in acute ischemic kidney injury, confirming the Pum2-Mff post-transcriptional axis in renal tubular cells. Western blot, overexpression in murine ischemia model, mitochondrial functional assays Cell biology and toxicology Medium 31993882
2022 Pum2 directly interacts with and affects the translation (but not mRNA levels or splicing) of Sox5, Bcl11b/Ctip2, and Rorβ mRNAs in mouse neocortical neurons, regulating area-specific cytoarchitecture and subcerebral connectivity. Transfection of primary neurons, in utero electroporation, retrograde labeling, single-molecule FISH, qRT-PCR, Pum2-Emx1-Cre conditional KO mice eLife High 35262486
2023 PUM2 directly binds DLX5 mRNA and represses its translation; PUM2 knockdown upregulates DLX5 expression and enhances osteogenic differentiation of mesenchymal stem cells. RNA immunoprecipitation, 3'UTR reporter assay, osteogenic differentiation assay, rat calvarial defect model Journal of biomedical science Medium 37088847
2020 PUM2 and PUM1 form distinct RNP regulatory networks in human male germ cells (TCam-2), associating with different protein cofactors (identified by mass spectrometry) and partially non-overlapping mRNA targets (identified by RIP-Seq), indicating functional divergence between paralogs. RIP-Seq, RNA-Seq, mass spectrometry-based interactome profiling Cells Medium 32316190
2019 LncRNA circ_0075932 binds directly to PUM2 protein; PUM2 positively regulates AuroraA kinase, which activates the NF-κB pathway to promote inflammation and apoptosis in dermal keratinocytes downstream of adipocyte-derived exosomes. RNA-protein direct binding assay, siRNA knockdown, NF-κB pathway assays, cell apoptosis/inflammation assays Biochemical and biophysical research communications Low 30824182
2021 PUM2 mediates packaging of miR-130a into exosomes in cancer-associated fibroblasts; overexpression/knockdown of PUM2 correspondingly promotes/inhibits exosomal miR-130a loading and xenograft tumor growth. Overexpression, knockdown, exosome isolation, miRNA quantification, xenograft mouse model International journal of nanomedicine Low 33542625
2022 PUM2 suppresses SIRT1 expression, which in turn inhibits SLC7A11 expression, thereby aggravating ferroptosis and neuroinflammation in cerebral ischemia-reperfusion injury. RT-qPCR, Western blot, knockdown in MCAO mouse model and OGD/R cell model, rescue assays Molecular and cellular biochemistry Low 35997855
2023 PUM2 binds EFEMP1 mRNA via immunoprecipitation and promotes EFEMP1 expression (stabilization), inhibiting vascular smooth muscle cell phenotypic switching (proliferation/migration) and preventing aortic dissection in an Ang-II mouse model. Immunoprecipitation of RNA, Western blot, overexpression vectors, Ang-II mouse model, immunofluorescence Experimental cell research Medium 37062520
2024 PUM2 facilitates degradation of NEDD4 mRNA by binding its 3'UTR, which prevents NEDD4-mediated ubiquitination and degradation of PTEN, thereby increasing PTEN levels and promoting chondrocyte ferroptosis through the Nrf2/HO-1 pathway. RNA immunoprecipitation, 3'UTR binding assay, Western blot, knockdown/overexpression, DMM mouse OA model Environmental toxicology Medium 38733337
2025 SENP1 directly deSUMOylates PUM2 (confirmed by Ni2+-NTA pull-down and co-IP), thereby enhancing PUM2 stability and expression. Stabilized PUM2 binds the 3'UTR of NRF2 mRNA, reducing NRF2 levels and diminishing transcriptional activation of HK1 and GLUT1, reducing glycolytic function in astrocytes. Ni2+-NTA agarose pull-down, co-immunoprecipitation, 3'UTR binding assay, APP/PS1 transgenic mouse model, knockdown rescue Cell biology and toxicology Medium 39794619
2025 PUM2-mediated repression of target mRNAs requires the CCR4-NOT deadenylase and is dependent on poly(A)-binding proteins PABPC1 and PABPC4; PUM2 associates with PABPCs, and increasing PABPC concentration inhibits PUM activity by stabilizing poly(A) tails. mRNA stability assays, co-immunoprecipitation of PUM with PABPCs, deadenylase requirement assays, PABPC titration experiments bioRxivpreprint Medium bio_10.1101_2025.10.02.680050
2025 PUM2 directly binds conserved Pumilio response elements (PREs) in SARS-CoV-2 transcripts, as shown by interactome data and PRE analysis, but neither PUM1 nor PUM2 affects progeny virion production. High-throughput interactome analysis, PRE mapping, PUM depletion and viral replication assays The Journal of general virology Medium 40956600
2024 PUM2 directly binds the 3'UTR of FOXO3 mRNA and inhibits its expression, promoting chondrocyte apoptosis in osteoarthritis. RNA immunoprecipitation, RNA pull-down, luciferase reporter, overexpression/knockdown, flow cytometry Heliyon Medium 38356524
2025 PUM2 suppresses stability of HDAC9 mRNA via direct binding (RIP and pull-down), attenuating HDAC9 expression and thereby reducing oxidative stress and promoting autophagy in diabetic contrast-induced acute kidney injury. RNA immunoprecipitation, RNA pull-down, mRNA stability assay, overexpression/knockdown, mouse DM-CIAKI model Diabetes & metabolism journal Medium 40930160
2025 The lncRNA Norad sequesters Pum2 protein, reducing its ability to bind and repress Mff mRNA translation. After ischemia-reperfusion, decreased Pum2 levels and binding to Mff mRNA (alongside increased Norad-Pum2 binding) lead to increased Mff protein, mitochondrial fragmentation, and neuronal injury; Pum2 overexpression restores mitochondrial morphology and reduces infarct volume. RNA-protein binding assays, OGD/R cell model, MCAO/R mouse model, Pum2 overexpression via viral vector Brain research Medium 39832611

Source papers

Stage 0 corpus · 52 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging. Molecular cell 121 30642763
2001 PUM2, a novel murine puf protein, and its consensus RNA-binding site. RNA (New York, N.Y.) 94 11780640
2002 Cloning and comparative sequence analysis of PUM1 and PUM2 genes, human members of the Pumilio family of RNA-binding proteins. Gene 77 12459267
2021 The BMSC-derived exosomal lncRNA Mir9-3hg suppresses cardiomyocyte ferroptosis in ischemia-reperfusion mice via the Pum2/PRDX6 axis. Nutrition, metabolism, and cardiovascular diseases : NMCD 75 34953631
2020 Pum2-Mff axis fine-tunes mitochondrial quality control in acute ischemic kidney injury. Cell biology and toxicology 69 31993882
2005 Identification and characterization of RNA sequences to which human PUMILIO-2 (PUM2) and deleted in Azoospermia-like (DAZL) bind. Genomics 68 15607425
2021 CAFs-Derived Exosomal miRNA-130a Confers Cisplatin Resistance of NSCLC Cells Through PUM2-Dependent Packaging. International journal of nanomedicine 64 33542625
2019 Circ_0075932 in adipocyte-derived exosomes induces inflammation and apoptosis in human dermal keratinocytes by directly binding with PUM2 and promoting PUM2-mediated activation of AuroraA/NF-κB pathway. Biochemical and biophysical research communications 54 30824182
2003 Mouse Pum1 and Pum2 genes, members of the Pumilio family of RNA-binding proteins, show differential expression in fetal and adult hematopoietic stem cells and progenitors. Blood cells, molecules & diseases 54 12667987
2019 Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell reports 46 30811992
2019 Pum2 Shapes the Transcriptome in Developing Axons through Retention of Target mRNAs in the Cell Body. Neuron 45 31606248
2018 RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3'UTR with miRNAs. Cell proliferation 41 30084199
2020 SUMOylation of PUM2 promotes the vasculogenic mimicry of glioma cells via regulating CEBPD. Clinical and translational medicine 33 32997416
2005 Interaction of the conserved meiotic regulators, BOULE (BOL) and PUMILIO-2 (PUM2). Molecular reproduction and development 33 15806553
2022 PUM2 aggravates the neuroinflammation and brain damage induced by ischemia-reperfusion through the SLC7A11-dependent inhibition of ferroptosis via suppressing the SIRT1. Molecular and cellular biochemistry 31 35997855
2019 RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 30 30909144
2019 LncRNA TUG1 aggravates the progression of cervical cancer by binding PUM2. European review for medical and pharmacological sciences 27 31646551
2019 RNA-binding protein PUM2 regulates mesenchymal stem cell fate via repression of JAK2 and RUNX2 mRNAs. Journal of cellular physiology 23 31595981
2021 Circular RNA RBM33 contributes to cervical cancer progression via modulation of the miR-758-3p/PUM2 axis. Journal of molecular histology 22 33398465
2020 Silencing SCAMP1-TV2 Inhibited the Malignant Biological Behaviors of Breast Cancer Cells by Interaction With PUM2 to Facilitate INSM1 mRNA Degradation. Frontiers in oncology 21 32670859
2011 A translational regulator, PUM2, promotes both protein stability and kinase activity of Aurora-A. PloS one 18 21589936
2019 PUM2 Promotes Glioblastoma Cell Proliferation and Migration via Repressing BTG1 Expression. Cell structure and function 17 30787206
2023 Downregulation of the RNA-binding protein PUM2 facilitates MSC-driven bone regeneration and prevents OVX-induced bone loss. Journal of biomedical science 15 37088847
2018 PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cellular and molecular life sciences : CMLS 15 30269240
2020 Pum2 mediates Sirt1 mRNA decay and exacerbates hypoxia/reoxygenation-induced cardiomyocyte apoptosis. Experimental cell research 14 32437714
2019 Demonstration of protein cooperativity mediated by RNA structure using the human protein PUM2. RNA (New York, N.Y.) 14 30914482
2005 P2P-R expression is genetically coregulated with components of the translation machinery and with PUM2, a translational repressor that associates with the P2P-R mRNA. Journal of cellular physiology 12 15617101
2020 Characterization of RNP Networks of PUM1 and PUM2 Post-Transcriptional Regulators in TCam-2 Cells, a Human Male Germ Cell Model. Cells 11 32316190
2024 Melatonin alleviates hyperoxia-induced lung injury through elevating MSC exosomal miR-18a-5p expression to repress PUM2 signaling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 10 39183539
2024 PUM2 promoted osteoarthritis progression through PTEN-mediated chondrocyte ferroptosis by facilitating NEDD4 mRNA degradation. Environmental toxicology 9 38733337
2022 Pum2 and TDP-43 refine area-specific cytoarchitecture post-mitotically and modulate translation of Sox5, Bcl11b, and Rorb mRNAs in developing mouse neocortex. eLife 8 35262486
2023 Hyperglycemia-induced endothelial exosomes trigger trophoblast dysregulation and abnormal placentation through PUM2-mediated repression of SOX2. Human & experimental toxicology 7 36607285
2023 PUM2 regulates the formation of thoracic aortic dissection through EFEMP1. Experimental cell research 6 37062520
2022 RNA binding protein PUM2 promotes hepatocellular carcinoma proliferation and apoptosis via binding to the 3'UTR of BTG3. Oncology letters 6 36072004
2025 Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2. BMC oral health 5 39979903
2023 PUF-8, a C. elegans ortholog of the RNA-binding proteins PUM1 and PUM2, is required for robustness of the cell death fate. Development (Cambridge, England) 5 37747106
2011 Spatial-temporal expression of pum1 and pum2 in medaka Oryzias latipes. Journal of fish biology 5 22220892
2024 LncRNA 51325 Alleviates Bone Cancer Induced Hyperalgesia Through Inhibition of Pum2. Journal of pain research 3 38249568
2025 Disruption of the Pum2 axis Aggravates neuronal damage following cerebral Ischemia-Reperfusion in mice. Brain research 2 39832611
2025 IGF2BP2 binding to CPSF6 facilitates m6A-mediated alternative polyadenylation of PUM2 and promotes malignant progression in ovarian cancer. Clinical and translational medicine 2 40629911
2025 PUM2 Lowers HDAC9 mRNA Stability to Improve Contrast-Induced Acute Kidney Injury through Attenuating Oxidative Stress and Promoting Autophagy. Diabetes & metabolism journal 2 40930160
2024 RNA binding protein PUM2 promotes IL-1β-induced apoptosis of chondrocytes via regulating FOXO3 expression. Heliyon 2 38356524
2024 Wnt/β-catenin Pathway Aggravates Renal Fibrosis by Activating PUM2 Transcription to Repress YME1L-mediated Mitochondrial Homeostasis. Biochemical genetics 2 38564095
2025 SENP1 inhibits aerobic glycolysis in Aβ1-42-incubated astrocytes by promoting PUM2 deSUMOylation. Cell biology and toxicology 1 39794619
2025 CircRERE promotes myocardial ischemia/reperfusion injury by increasing UHRF1 mRNA decay through targeting PUM2 to reduce Drp1 promoter methylation. Chinese medical journal 1 40887836
2025 PUM2 binds SARS-CoV-2 RNA and PUM1 mildly reduces viral RNA levels, but neither protein affects progeny virus production. The Journal of general virology 1 40956600
2023 RNA-binding protein PUM2 promotes T-cell acute lymphoblastic leukemia via competitively binding to RBM5 3'UTR with miR-28-5p. European journal of haematology 1 36536516
2026 PUM2 knockdown regulates the expression and alternative splicing of genes associated with myocardial fibrosis in H9C2 cells. Experimental and therapeutic medicine 0 41694110
2026 Loss of Pum2 exacerbates colitis by disrupting macrophage-epithelial crosstalk and promoting epithelial necroptosis. Cell death discovery 0 41856997
2025 PUM2 binds SARS-CoV-2 RNA and PUM1 mildly reduces viral RNA levels, but neither protein affects progeny virus production. bioRxiv : the preprint server for biology 0 40666984
2024 MiR-495-3p promotes cardiac hypertrophy by targeting Pum2. Cellular and molecular biology (Noisy-le-Grand, France) 0 39262254
2023 Norad Competently Binds with Pum2 to Regulate Neuronal Apoptosis and Play a Neuroprotective Role After SAH in Mice. Neuroscience 0 37913857