Affinage

PUM2

Pumilio homolog 2 · UniProt Q8TB72

Length
1066 aa
Mass
114.2 kDa
Annotated
2026-06-10
52 papers in source corpus 36 papers cited in narrative 36 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PUM2 is a conserved PUF-family sequence-specific RNA-binding protein that uses its C-terminal PUM homology domain to recognize a defined Pumilio-binding element (PBE, consensus UGUANAUA...) in target mRNA 3'UTRs with nanomolar affinity, thereby controlling translation and stability of broad regulatory programs (PMID:11780640). Cooperative occupancy of multi-PBE targets is shaped by RNA secondary structure, which redistributes conformational states to favor or impede additional PUM2 binding (PMID:30914482). Mechanistically, PUM2-mediated repression requires the CCR4-NOT deadenylase and depends on poly(A)-binding proteins PABPC1/PABPC4: PUM2 associates with PABPCs and high PABPC levels protect poly(A) tails to limit PUM2 activity [PMID:bio_10.1101_2025.10.02.680050]. Through this 3'UTR-directed repression PUM2 governs mitochondrial fission and mitophagy by repressing Mff mRNA (PMID:30642763), cell-cycle progression by repressing Cdkn1b/p27 (PMID:30811992), neuronal cytoarchitecture by translationally controlling Sox5, Bcl11b, and Rorβ in developing neocortex (PMID:35262486), somatodendritic exclusion of PBE-containing mRNAs from axons (PMID:31606248), and stem-cell fate via repression of RUNX2, JAK2, and DLX5 (PMID:31595981, PMID:37088847). Beyond repression, PUM2 can stabilize transcripts by competing with miRNAs for shared 3'UTR sites (PMID:30084199, PMID:30909144), and during mitosis it relocalizes from the cytoplasm to centrosomes where it binds the Aurora-A D-box, protecting Aurora-A from APC/C(Cdh1)-mediated degradation while enhancing its kinase activity (PMID:21589936). PUM2 protein levels and activity are themselves controlled by SUMOylation through UBE2I (reversed by SENP1) (PMID:32997416, PMID:39794619) and by sequestration through lncRNAs including Norad, which titrates PUM2 away from Mff mRNA (PMID:39832611); PUM2 transcript abundance is further set by m6A-dependent alternative polyadenylation driven by IGF2BP2/CPSF6 (PMID:40629911). PUM2 forms paralog-distinct and cofactor-specific RNP networks together with PUM1, DAZL, and BOULE, with protein partners helping determine target specificity (PMID:15607425, PMID:15806553, PMID:32316190).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2001 High

    Establishing that human PUM2 is a bona fide sequence-specific RNA binder defined the molecular basis for all downstream target recognition.

    Evidence SELEX and recombinant protein binding assays defining the PBE consensus and nanomolar affinity

    PMID:11780640

    Open questions at the time
    • Did not identify endogenous mRNA targets
    • No structural model of the PUM-HD bound to RNA in this study
  2. 2005 Medium

    Identifying PUM2 protein partners and the first mammalian mRNA target showed that PUM2 acts within combinatorial RNP complexes whose composition tunes target specificity.

    Evidence Reciprocal Co-IP and RNA-binding assays for DAZL and BOULE, plus pull-down/RT-PCR confirming the P2P-R mRNA target

    PMID:15607425 PMID:15617101 PMID:15806553

    Open questions at the time
    • Functional consequence of PUM2-DAZL/BOULE complexes on translation not quantified
    • Single-lab Co-IP evidence for partners
  3. 2011 High

    Discovery of a centrosomal, kinase-stabilizing role revealed that PUM2 has a non-canonical, RNA-independent moonlighting function in mitosis.

    Evidence Co-IP, ubiquitination and kinase activity assays, and immunofluorescence showing PUM2 binds the Aurora-A D-box and blocks APC/C(Cdh1) degradation

    PMID:21589936

    Open questions at the time
    • Structural basis of D-box recognition not resolved
    • Relationship between RNA-binding and centrosomal functions unknown
  4. 2019 High

    Genetic loss-of-function across species defined physiological repression programs, establishing PUM2 control of mitochondrial fission (Mff), proliferation (Cdkn1b), and axonal mRNA exclusion.

    Evidence CRISPR/Cas9 knockouts in mouse and C. elegans, gene-dosage and Cdkn1b epistasis in mice, and reporter mRNA localization with Pum2 knockdown in neurons

    PMID:30642763 PMID:30811992 PMID:31606248

    Open questions at the time
    • Direct deadenylation/repression mechanism not yet biochemically dissected in these systems
    • Tissue-specific target hierarchies incompletely mapped
  5. 2019 High

    Quantitative binding studies showed that PUM2 occupancy of multi-site targets is not independent but governed by RNA structural cooperativity.

    Evidence Equilibrium binding across a 68-RNA panel with statistical mechanical modeling

    PMID:30914482

    Open questions at the time
    • Whether RNA structural cooperativity operates on endogenous targets in cells not shown
  6. 2019 Medium

    PUM2 was shown to also act as a stabilizer by competing with miRNAs for shared 3'UTR sites, broadening its regulatory repertoire beyond repression.

    Evidence RIP/RIP-seq and luciferase assays showing PUM2 displaces miR-590-3p/miR-9 (STARD13) and miR-376a (NRP-1)

    PMID:30084199 PMID:30909144

    Open questions at the time
    • Determinants of repressive versus stabilizing outcome not defined
    • Competition shown in cancer cell lines only
  7. 2020 Medium

    Mapping paralog-specific RNP networks distinguished PUM2 from PUM1 in target pools and protein cofactors.

    Evidence RIP-Seq, RNA-Seq, and mass-spectrometry proteomics in human germ cells

    PMID:30269240 PMID:32316190

    Open questions at the time
    • Mechanistic basis of paralog-specific cofactor recruitment unresolved
  8. 2022 High

    PUM2 was established as a post-mitotic translational regulator that shapes neocortical area identity and connectivity.

    Evidence Pum2 KO mice, in utero electroporation, retrograde tracing, and translational reporter assays for Sox5/Bcl11b/Rorβ

    PMID:35262486

    Open questions at the time
    • Upstream control of PUM2 activity timing in cortical neurons unknown
  9. 2024 Medium

    Defining SUMOylation/deSUMOylation control showed PUM2 abundance and activity are post-translationally gated by the SUMO machinery.

    Evidence Co-IP, Ni2+-NTA pull-down, and RIP showing UBE2I-mediated SUMOylation destabilizes PUM2 while SENP1 deSUMOylation stabilizes it

    PMID:32997416 PMID:39794619

    Open questions at the time
    • SUMO acceptor residues not mapped
    • How SUMO state alters target selection unclear
  10. 2025 Medium

    Reconstitution of the repression machinery defined the effector mechanism: PUM2 represses via CCR4-NOT in a PABPC-dependent manner.

    Evidence mRNA stability assays, PUM2-PABPC association, PABPC depletion/overexpression, and deadenylase mutant analysis (preprint)

    PMID:bio_10.1101_2025.10.02.680050

    Open questions at the time
    • Preprint, not yet peer-reviewed
    • Structural picture of the PUM2-CCR4-NOT-PABPC assembly absent
  11. 2025 Medium

    lncRNA sequestration and m6A-dependent alternative polyadenylation were shown to regulate PUM2 availability and transcript stability, adding upstream layers of control.

    Evidence RIP and in vivo models for Norad sequestration from Mff mRNA, and PAS-seq/eCLIP/RIP-seq showing IGF2BP2-CPSF6 m6A-driven proximal polyadenylation of PUM2 pre-mRNA

    PMID:39832611 PMID:40629911

    Open questions at the time
    • Quantitative contribution of lncRNA titration to physiological PUM2 output not established

Open questions

Synthesis pass · forward-looking unresolved questions
  • How PUM2's RNA-dependent repressor function and its RNA-independent centrosomal/kinase-activating role are coordinated within a single protein remains unresolved.
  • No structural model linking PUM-HD RNA binding to Aurora-A interaction
  • Cell-cycle switch governing PUM2 cytoplasmic-to-centrosomal relocalization unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 4 GO:0045182 translation regulator activity 4 GO:0098772 molecular function regulator activity 1
Localization
GO:0005815 microtubule organizing center 1 GO:0005829 cytosol 1
Pathway
R-HSA-1266738 Developmental Biology 3 R-HSA-1640170 Cell Cycle 2 R-HSA-8953854 Metabolism of RNA 2
Partners
AURKABOLLDAZLPABPC1PABPC4PUM1SENP1UBE2I
Complex memberships
CCR4-NOT

Evidence

Reading pass · 36 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 PUM2 contains a C-terminal PUM homology domain (PUM-HD) that binds RNA with high affinity (Kd ~6.5 nM); SELEX identified a consensus PUM2-binding element (PBE) [UGUANAUARNNNNBBBBSCCS] related to but distinct from the Drosophila Nanos response element. SELEX (iterative amplification-selection), recombinant protein binding assay RNA (New York, N.Y.) High 11780640
2005 Human PUM2 specifically recognizes the Drosophila Pumilio NRE RNA target; single-nucleotide changes in the NRE abolish PUM2 binding. PUM2 co-immunoprecipitates with DAZL and both proteins can bind the same RNA target (SDAD1 mRNA 3'UTR), forming a complex. Co-immunoprecipitation, RNA binding assay, mutagenesis of NRE Genomics Medium 15607425
2005 Human BOULE (BOL) forms homodimers and interacts with PUM2; the dimerization domain of BOL is required for interaction with PUM2. BOL and PUM2 form a complex on a subset of PUM2 RNA targets distinct from the PUM2-DAZL complex, indicating that protein interactions determine RNA target specificity. Co-immunoprecipitation, domain mapping, RNA binding assay Molecular reproduction and development Medium 15806553
2005 PUM2 binds P2P-R mRNA via PUM2-binding elements in its 3'UTR; PUM2 pull-down confirmed physical association with P2P-R mRNA, representing the first confirmed mammalian mRNA target for a PUF protein. PUM2 pull-down combined with RT-PCR, 3'UTR sequence analysis Journal of cellular physiology Medium 15617101
2011 PUM2 physically binds the D-box of Aurora-A kinase, protecting it from APC/C(Cdh1)-mediated ubiquitination and degradation, while also enhancing Aurora-A kinase activity. In interphase, PUM2 is cytoplasmic and acts as a translational repressor; in mitosis it localizes to centrosomes and associates with Aurora-A. Co-immunoprecipitation, ubiquitination assay, kinase activity assay, overexpression/depletion, immunofluorescence localization PloS one High 21589936
2018 PUM2 represses translation of RUNX2 and JAK2 mRNAs by directly binding their 3'UTRs; depletion of PUM2 in mesenchymal stem cells blocks adipogenesis and enhances osteogenesis, and CRISPR/Cas9 Pum2 silencing in zebrafish inhibits lipid accumulation and induces excessive bone formation. 3'UTR luciferase reporter assay, RNA immunoprecipitation, CRISPR/Cas9 gene silencing, in vivo zebrafish model Journal of cellular physiology High 31595981
2018 PUM1 and PUM2 show mechanistic differences in regulation of SIAH1 3'UTR: PUM1 represses SIAH1 in a PBE-independent manner whereas PUM2 requires PBEs. Their PUF domains form distinct EMSA complex patterns with SIAH1 3'UTRs. NANOS3, but not NANOS2, directly binds SIAH1 3'UTR independently of PBEs or PUF domain, representing first evidence of direct RNA binding by a NANOS protein. Luciferase reporter assay, EMSA, mutagenesis, NANOS mutant constructs Cellular and molecular life sciences : CMLS Medium 30269240
2019 PUM2 inhibits translation of Mff (mitochondrial fission factor) mRNA, thereby impairing mitochondrial fission and mitophagy. PUM2 levels increase with aging. CRISPR/Cas9-mediated Pum2 knockout in mouse muscles of elderly animals enhances mitochondrial fission and mitophagy and improves mitochondrial quality control. Multi-omics, translational profiling, CRISPR/Cas9 knockout, C. elegans genetic model (puf-8), mitochondrial morphology assays Molecular cell High 30642763
2019 PUM2 represses translation of Cdkn1b (p27) by binding PBEs in the Cdkn1b 3'UTR, promoting G1-S transition and cell proliferation. Pum1/Pum2 double mutant mice show gene dosage-dependent reductions in body and organ size; Cdkn1b deficiency partially rescues postnatal growth defects. PUM1 and PUM2 engage in auto-regulatory and reciprocal post-transcriptional repression. Genetic mouse knockout (null mutations), 3'UTR binding assays, genetic epistasis (Cdkn1b rescue) Cell reports High 30811992
2019 Pum2 is restricted to the soma of developing neurons and prevents PBE-containing mRNAs from entering axons. Introduction of PBEs into mRNAs with a β-actin zipcode prevents axonal localization and translation. Pum2 knockdown causes PBE-containing mRNAs to appear and be translated in axons, and results in axonal growth/branching defects in vivo and impaired axon regeneration in vitro. Reporter mRNA localization assay, Pum2 knockdown, in vivo neuronal imaging, axon regeneration assay Neuron High 31606248
2019 PUM2 directly binds STARD13 3'UTR and competitively outcompetes miR-590-3p and miR-9 for binding, thereby protecting STARD13 mRNA and inhibiting osteosarcoma cell proliferation, migration, and stemness. RNA immunoprecipitation (RIP) with RNA-seq, luciferase reporter assay Cell proliferation Medium 30084199
2019 PUM2 binds NRP-1 mRNA 3'UTR and competitively displaces miR-376a, increasing NRP-1 mRNA stability and expression, thereby promoting breast cancer cell stemness. RNA immunoprecipitation (RIP), luciferase reporter assay, knockdown/rescue experiments Biomedicine & pharmacotherapy Medium 30909144
2019 PUM2 directly binds BTG1 3'UTR as shown by RNA pull-down and RIP assays, repressing BTG1 expression and promoting glioblastoma cell proliferation and migration; BTG1 knockdown rescues the effect of PUM2 knockdown. RNA pull-down assay, RNA immunoprecipitation, shRNA knockdown, rescue experiment Cell structure and function Medium 30787206
2019 PUM2 is subject to SUMO2/3 modification; the SUMO E2 ligase UBE2I promotes PUM2 SUMOylation, which decreases PUM2 protein stability and its inhibitory effect on CEBPD mRNA, thereby inducing glioma vasculogenic mimicry. Co-immunoprecipitation, immunofluorescence, RIP assay, ChIP assay, luciferase assay Clinical and translational medicine Medium 32997416
2019 PUM2 cooperativity in RNA binding is mediated by RNA secondary structure: binding of one PUM2 molecule redistributes RNA conformational states to enhance or inhibit access of additional PUM2 molecules. Equilibrium binding measurements across 68 RNAs with two to five PBEs demonstrated this RNA structural cooperativity (RSC) mechanism. Equilibrium binding measurements, statistical mechanical modeling, 68-RNA panel with varying structure stabilities RNA (New York, N.Y.) High 30914482
2020 Pum2 mediates Sirt1 mRNA decay in cardiomyocytes via two PUM2 binding elements in the Sirt1 3'UTR; Pum2 overexpression promotes acetylation of LKB1 (by reducing Sirt1 mRNA), repressing AMPK pathway activity and promoting H/R-induced apoptosis. RIP, mRNA stability assay, overexpression/knockdown, western blot for AMPK/LKB1 pathway, rescue experiments Experimental cell research Medium 32437714
2020 The Pum2-Mff axis regulates mitochondrial quality control in acute ischemic kidney injury: Pum2 levels are inversely correlated with Mff protein, and Pum2 overexpression reduces ischemia-induced Mff upregulation, preserving mitochondrial homeostasis and protecting renal tubules. Western blot, overexpression in mouse model, mitochondrial respiration assay Cell biology and toxicology Medium 31993882
2020 PUM2 interacts with SCAMP1-TV2 lncRNA; when SCAMP1-TV2 is downregulated, PUM2 binds INSM1 mRNA, promoting its degradation. RNA immunoprecipitation (RIP), RNA pull-down, co-immunoprecipitation Frontiers in oncology Low 32670859
2020 PUM1 and PUM2 form partially distinct RNP networks in human male germ cells (TCam-2): RIP-Seq identified different target mRNA pools and mass spectrometry identified distinct protein cofactors for PUM1 and PUM2, indicating paralog-specific regulation. RIP-Seq, RNA-Seq, mass spectrometry proteomics Cells Medium 32316190
2021 PUM2 mediates packaging of miRNA-130a into exosomes in cancer-associated fibroblasts; PUM2 overexpression promotes and knockdown inhibits tumor growth in xenograft mice. Knockdown/overexpression, xenograft assay, exosome isolation and characterization International journal of nanomedicine Low 33542625
2021 PUM2 binds PRDX6 promoter (via ChIP) and represses PRDX6 expression; lncRNA Mir9-3hg binds PUM2 and downregulates it, relieving PRDX6 repression and inhibiting ferroptosis in cardiomyocytes. RNA binding protein immunoprecipitation, chromatin immunoprecipitation (ChIP) Nutrition, metabolism, and cardiovascular diseases Medium 34953631
2022 Pum2 directly binds and represses translation of Sox5, Bcl11b/Ctip2, and Rorβ mRNAs in developing mouse neocortex post-mitotically, shaping area-specific cytoarchitecture. Loss of Pum2 leads to expansion of subcerebral projection neurons (Sox5+Bcl11b+) at expense of layer IV neurons (Rorβ+) in somatosensory cortex, with corresponding changes in subcerebral connectivity. In utero electroporation, retrograde labeling, Pum2 KO mice (Pum2;Emx1-Cre), translational reporter assays, qRT-PCR, smFISH (negative for mRNA level change) eLife High 35262486
2022 PUM2 aggravates I/R-induced neuroinflammation and brain damage by suppressing SIRT1 expression, which leads to reduced SLC7A11 levels and thereby promotes ferroptosis in neurons. MCAO/R mouse model, OGD/R cell model, knockdown/rescue, western blot, RT-qPCR Molecular and cellular biochemistry Low 35997855
2022 PUM2 represses BTG3 expression by directly binding to the BTG3 3'UTR; PUM2 silencing inhibits proliferation and promotes apoptosis of HCC cells, effects reversed by BTG3 knockdown. Luciferase reporter assay, RNA binding protein immunoprecipitation, knockdown/rescue Oncology letters Medium 36072004
2023 PUM2 directly binds DLX5 mRNA (confirmed by RNA-IP) and represses its translation; PUM2 knockdown upregulates DLX5 and enhances osteogenic differentiation of mesenchymal stem cells. RNA-immunoprecipitation, 3'UTR reporter assay, osteogenic differentiation assays, in vivo calvarial defect model Journal of biomedical science Medium 37088847
2023 PUM2 promotes NEDD4 mRNA degradation by binding to the NEDD4 mRNA 3'UTR, leading to reduced NEDD4-mediated PTEN ubiquitination/degradation, PTEN accumulation, and ferroptosis in chondrocytes, exacerbating osteoarthritis progression. RNA immunoprecipitation, RNA pulldown, western blot, in vivo intra-articular injection model Environmental toxicology Medium 38733337
2023 PUM2 binds EFEMP1 mRNA (confirmed by immunoprecipitation) to promote EFEMP1 expression; PUM2 overexpression inhibits VSMC proliferation/migration and prevents aortic dissection in an angiotensin II mouse model. Immunoprecipitation/RNA-IP, western blot, mouse aortic dissection model Experimental cell research Low 37062520
2023 PUM2 represses SOX2 mRNA stability via interaction with SOX2 mRNA; PUM2 is upregulated in hyperglycemia-induced endothelial exosomes and delivered to trophoblasts, impairing their proliferation and invasion. High-throughput RNA-seq, exosome isolation, PUM2-mRNA interaction assay, SOX2 rescue experiment Human & experimental toxicology Low 36607285
2024 SENP1 deSUMOylates PUM2 (confirmed by Ni2+-NTA pull-down and Co-IP), enhancing PUM2 stability; PUM2 then binds NRF2 mRNA 3'UTR and reduces NRF2 levels, diminishing transcription of glycolytic enzymes HK1 and GLUT1 in astrocytes under Alzheimer's disease conditions. Ni2+-NTA agarose bead pull-down, co-immunoprecipitation, RIP, luciferase reporter, APP/PS1 mouse model Cell biology and toxicology Medium 39794619
2024 PUM2 binds FOXO3 mRNA 3'UTR (confirmed by RIP, RNA pull-down, and luciferase assay) and represses FOXO3 translation, promoting IL-1β-induced chondrocyte apoptosis and ROS generation. RNA immunoprecipitation, RNA pull-down, luciferase reporter assay, overexpression/knockdown Heliyon Medium 38356524
2024 PUM2 destabilizes HDAC9 mRNA via direct binding (confirmed by RIP and RNA pull-down); PUM2 overexpression suppresses oxidative stress and promotes autophagy in DM-CIAKI by reducing HDAC9 expression. RNA immunoprecipitation, RNA pull-down, in vivo DM-CIAKI mouse model, western blot Diabetes & metabolism journal Medium 40930160
2025 PUM2 directly binds SARS-CoV-2 RNA at conserved PREs; however, altering PUM2 levels does not affect progeny virion production. This is a negative mechanistic finding for antiviral function. RNA binding assay, PUM2 depletion/overexpression, viral titer measurement The Journal of general virology Medium 40956600
2025 PUM2-mediated mRNA repression requires CCR4-NOT deadenylase and depends on PABPC1 and PABPC4: PUM2 associates with PABPCs, and in their absence mRNA targets become unstable independently of PUM. Increasing PABPC levels inhibits PUM2 activity in a concentration-dependent manner by protecting poly(A) tails from deadenylation. mRNA stability assay, PUM2 association assay, PABPC depletion/overexpression, deadenylase mutant analysis bioRxiv (preprint)preprint Medium bio_10.1101_2025.10.02.680050
2025 CircRERE promotes UHRF1 mRNA decay by interacting with PUM2 (validated by RNA-IP and RNA pull-down); this reduces UHRF1-mediated methylation of the Drp1 promoter, increasing Drp1 expression and mitochondrial fission/dysfunction in myocardial I/R injury. RNA immunoprecipitation, RNA pull-down, ChIP assay, I/R mouse model Chinese medical journal Medium 40887836
2025 The Norad lncRNA sequesters Pum2 via Pum2-recognition sequences, reducing Pum2 binding to Mff mRNA; in neurons after OGD/R, Norad levels increase and Pum2 binding to Mff mRNA decreases, elevating Mff protein and causing mitochondrial fragmentation. Pum2 overexpression reduces Mff levels, restores mitochondrial morphology, and improves outcomes in MCAO/R mice. RIP, overexpression in OGD/R and MCAO/R models, mitochondrial morphology assay, neurobehavioral assessment Brain research Medium 39832611
2025 IGF2BP2 KH1-4 domains directly bind the CPSF6-RS domain (confirmed by GST pull-down and IP/MS), recruiting CPSF6 to m6A-modified PUM2 pre-mRNA and promoting use of a proximal polyadenylation signal, generating a shorter, more stable PUM2 transcript that promotes malignant progression of ovarian cancer. RNA pull-down with biotin-labelled m6A, immunoprecipitation, mass spectrometry, GST pull-down, PAS-seq, eCLIP-seq, RIP-seq, meRIP-seq, 3'RACE, RNA stability assay, dual luciferase reporter Clinical and translational medicine High 40629911

Source papers

Stage 0 corpus · 52 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging. Molecular cell 122 30642763
2001 PUM2, a novel murine puf protein, and its consensus RNA-binding site. RNA (New York, N.Y.) 94 11780640
2002 Cloning and comparative sequence analysis of PUM1 and PUM2 genes, human members of the Pumilio family of RNA-binding proteins. Gene 78 12459267
2021 The BMSC-derived exosomal lncRNA Mir9-3hg suppresses cardiomyocyte ferroptosis in ischemia-reperfusion mice via the Pum2/PRDX6 axis. Nutrition, metabolism, and cardiovascular diseases : NMCD 77 34953631
2020 Pum2-Mff axis fine-tunes mitochondrial quality control in acute ischemic kidney injury. Cell biology and toxicology 70 31993882
2005 Identification and characterization of RNA sequences to which human PUMILIO-2 (PUM2) and deleted in Azoospermia-like (DAZL) bind. Genomics 68 15607425
2021 CAFs-Derived Exosomal miRNA-130a Confers Cisplatin Resistance of NSCLC Cells Through PUM2-Dependent Packaging. International journal of nanomedicine 64 33542625
2019 Circ_0075932 in adipocyte-derived exosomes induces inflammation and apoptosis in human dermal keratinocytes by directly binding with PUM2 and promoting PUM2-mediated activation of AuroraA/NF-κB pathway. Biochemical and biophysical research communications 55 30824182
2003 Mouse Pum1 and Pum2 genes, members of the Pumilio family of RNA-binding proteins, show differential expression in fetal and adult hematopoietic stem cells and progenitors. Blood cells, molecules & diseases 54 12667987
2019 Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell reports 48 30811992
2019 Pum2 Shapes the Transcriptome in Developing Axons through Retention of Target mRNAs in the Cell Body. Neuron 46 31606248
2018 RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3'UTR with miRNAs. Cell proliferation 41 30084199
2020 SUMOylation of PUM2 promotes the vasculogenic mimicry of glioma cells via regulating CEBPD. Clinical and translational medicine 34 32997416
2005 Interaction of the conserved meiotic regulators, BOULE (BOL) and PUMILIO-2 (PUM2). Molecular reproduction and development 33 15806553
2022 PUM2 aggravates the neuroinflammation and brain damage induced by ischemia-reperfusion through the SLC7A11-dependent inhibition of ferroptosis via suppressing the SIRT1. Molecular and cellular biochemistry 31 35997855
2019 RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 30 30909144
2019 LncRNA TUG1 aggravates the progression of cervical cancer by binding PUM2. European review for medical and pharmacological sciences 28 31646551
2019 RNA-binding protein PUM2 regulates mesenchymal stem cell fate via repression of JAK2 and RUNX2 mRNAs. Journal of cellular physiology 23 31595981
2021 Circular RNA RBM33 contributes to cervical cancer progression via modulation of the miR-758-3p/PUM2 axis. Journal of molecular histology 22 33398465
2020 Silencing SCAMP1-TV2 Inhibited the Malignant Biological Behaviors of Breast Cancer Cells by Interaction With PUM2 to Facilitate INSM1 mRNA Degradation. Frontiers in oncology 21 32670859
2011 A translational regulator, PUM2, promotes both protein stability and kinase activity of Aurora-A. PloS one 19 21589936
2019 PUM2 Promotes Glioblastoma Cell Proliferation and Migration via Repressing BTG1 Expression. Cell structure and function 18 30787206
2018 PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cellular and molecular life sciences : CMLS 16 30269240
2023 Downregulation of the RNA-binding protein PUM2 facilitates MSC-driven bone regeneration and prevents OVX-induced bone loss. Journal of biomedical science 15 37088847
2020 Pum2 mediates Sirt1 mRNA decay and exacerbates hypoxia/reoxygenation-induced cardiomyocyte apoptosis. Experimental cell research 15 32437714
2019 Demonstration of protein cooperativity mediated by RNA structure using the human protein PUM2. RNA (New York, N.Y.) 14 30914482
2020 Characterization of RNP Networks of PUM1 and PUM2 Post-Transcriptional Regulators in TCam-2 Cells, a Human Male Germ Cell Model. Cells 12 32316190
2005 P2P-R expression is genetically coregulated with components of the translation machinery and with PUM2, a translational repressor that associates with the P2P-R mRNA. Journal of cellular physiology 12 15617101
2024 Melatonin alleviates hyperoxia-induced lung injury through elevating MSC exosomal miR-18a-5p expression to repress PUM2 signaling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 11 39183539
2024 PUM2 promoted osteoarthritis progression through PTEN-mediated chondrocyte ferroptosis by facilitating NEDD4 mRNA degradation. Environmental toxicology 10 38733337
2022 Pum2 and TDP-43 refine area-specific cytoarchitecture post-mitotically and modulate translation of Sox5, Bcl11b, and Rorb mRNAs in developing mouse neocortex. eLife 9 35262486
2023 Hyperglycemia-induced endothelial exosomes trigger trophoblast dysregulation and abnormal placentation through PUM2-mediated repression of SOX2. Human & experimental toxicology 7 36607285
2023 PUM2 regulates the formation of thoracic aortic dissection through EFEMP1. Experimental cell research 6 37062520
2022 RNA binding protein PUM2 promotes hepatocellular carcinoma proliferation and apoptosis via binding to the 3'UTR of BTG3. Oncology letters 6 36072004
2025 Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2. BMC oral health 5 39979903
2023 PUF-8, a C. elegans ortholog of the RNA-binding proteins PUM1 and PUM2, is required for robustness of the cell death fate. Development (Cambridge, England) 5 37747106
2011 Spatial-temporal expression of pum1 and pum2 in medaka Oryzias latipes. Journal of fish biology 5 22220892
2024 RNA binding protein PUM2 promotes IL-1β-induced apoptosis of chondrocytes via regulating FOXO3 expression. Heliyon 4 38356524
2025 IGF2BP2 binding to CPSF6 facilitates m6A-mediated alternative polyadenylation of PUM2 and promotes malignant progression in ovarian cancer. Clinical and translational medicine 3 40629911
2024 LncRNA 51325 Alleviates Bone Cancer Induced Hyperalgesia Through Inhibition of Pum2. Journal of pain research 3 38249568
2024 Wnt/β-catenin Pathway Aggravates Renal Fibrosis by Activating PUM2 Transcription to Repress YME1L-mediated Mitochondrial Homeostasis. Biochemical genetics 3 38564095
2025 Disruption of the Pum2 axis Aggravates neuronal damage following cerebral Ischemia-Reperfusion in mice. Brain research 2 39832611
2025 CircRERE promotes myocardial ischemia/reperfusion injury by increasing UHRF1 mRNA decay through targeting PUM2 to reduce Drp1 promoter methylation. Chinese medical journal 2 40887836
2025 PUM2 Lowers HDAC9 mRNA Stability to Improve Contrast-Induced Acute Kidney Injury through Attenuating Oxidative Stress and Promoting Autophagy. Diabetes & metabolism journal 2 40930160
2025 SENP1 inhibits aerobic glycolysis in Aβ1-42-incubated astrocytes by promoting PUM2 deSUMOylation. Cell biology and toxicology 1 39794619
2025 PUM2 binds SARS-CoV-2 RNA and PUM1 mildly reduces viral RNA levels, but neither protein affects progeny virus production. The Journal of general virology 1 40956600
2023 RNA-binding protein PUM2 promotes T-cell acute lymphoblastic leukemia via competitively binding to RBM5 3'UTR with miR-28-5p. European journal of haematology 1 36536516
2026 PUM2 knockdown regulates the expression and alternative splicing of genes associated with myocardial fibrosis in H9C2 cells. Experimental and therapeutic medicine 0 41694110
2026 Loss of Pum2 exacerbates colitis by disrupting macrophage-epithelial crosstalk and promoting epithelial necroptosis. Cell death discovery 0 41856997
2025 PUM2 binds SARS-CoV-2 RNA and PUM1 mildly reduces viral RNA levels, but neither protein affects progeny virus production. bioRxiv : the preprint server for biology 0 40666984
2024 MiR-495-3p promotes cardiac hypertrophy by targeting Pum2. Cellular and molecular biology (Noisy-le-Grand, France) 0 39262254
2023 Norad Competently Binds with Pum2 to Regulate Neuronal Apoptosis and Play a Neuroprotective Role After SAH in Mice. Neuroscience 0 37913857

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