Affinage

PABPC1

Polyadenylate-binding protein 1 · UniProt P11940

Length
636 aa
Mass
70.7 kDa
Annotated
2026-04-29
100 papers in source corpus 30 papers cited in narrative 30 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PABPC1 is the principal cytoplasmic poly(A)-binding protein that coordinates mRNA translation, stability, and decay by serving as a platform for competing interactions at the mRNA 3' poly(A) tail. It stimulates translation initiation by bridging the poly(A) tail to the eIF4G–eIF4F cap complex, and its proximity to a stop codon suppresses nonsense-mediated mRNA decay (NMD), establishing a positional checkpoint for premature termination codon recognition (PMID:17318186, PMID:21989405). PABPC1-dependent deadenylation is activated when BTG1/BTG2 APRO domains engage its RRM1 to present the poly(A) 3' end to the CAF1/CCR4-NOT catalytic center, while GW182/TNRC6 proteins compete with eIF4G for PABPC1 binding to promote miRNA-mediated silencing (PMID:26912148, PMID:35640718, PMID:19797087). Post-translational modifications—including CARM1-mediated arginine methylation, MKRN3-catalyzed non-proteolytic K27/K29-linked ubiquitination (reversed by USP10), and stress-induced SUMOylation that recruits TIA1 and U-rich mRNAs into stress granules—tune its translational and mRNA-triage functions, and de novo PABPC1 missense variants that disrupt PAIP2 binding impair cortical neurogenesis (PMID:11850402, PMID:34143182, PMID:37757903, PMID:40774970, PMID:35511136).

Mechanistic history

Synthesis pass · year-by-year structured walk · 21 steps
  1. 2002 High

    Identifying PABPC1 as a CARM1 substrate established that post-translational arginine methylation modifies this core translation factor, opening questions about how methylation tunes its RNA-binding or protein-interaction properties.

    Evidence High-density protein array methylation screen with in vivo confirmation

    PMID:11850402

    Open questions at the time
    • Functional consequence of CARM1-mediated methylation on PABPC1 translation activity or partner binding not determined
    • Specific arginine residues methylated in vivo not mapped at single-residue resolution
  2. 2003 High

    Demonstrating that influenza NS1 binds PABPC1 in an RNA-independent manner without displacing eIF4G revealed that viruses can co-opt the PABPC1–eIF4G translation axis by forming a trimeric complex to redirect translation toward viral mRNAs.

    Evidence Co-IP in infected cells and GST pulldown with domain mapping

    PMID:14645908

    Open questions at the time
    • Whether the NS1–PABPC1–eIF4G complex directly enhances polysome loading on viral mRNA was not tested
    • Structural basis of the trimeric interaction unknown
  3. 2007 High

    Tethering PABPC1 downstream of a premature termination codon abolished NMD, establishing that PABPC1 position—not just poly(A) tail presence—is the critical determinant distinguishing normal from premature stop codons.

    Evidence Tethering assay and RNAi depletion with NMD reporter in Drosophila

    PMID:17318186

    Open questions at the time
    • Mechanism by which PABPC1 proximity antagonizes UPF1 surveillance complex not defined
    • Whether mammalian NMD uses the identical positional rule was inferred but not directly shown here
  4. 2009 High

    Showing that GW182 competes with eIF4G for PABPC1 binding and recruits CCR4-NOT revealed the molecular switch by which miRNA silencing displaces the translation-promoting eIF4G–PABPC1 interaction and redirects PABPC1-bound mRNAs toward deadenylation.

    Evidence Competition binding, co-IP, tethering, and overexpression/knockdown reporter assays in Drosophila S2 cells

    PMID:19797087

    Open questions at the time
    • Stoichiometry and kinetics of GW182 vs. eIF4G competition on endogenous mRNAs not measured
  5. 2010 High

    Mapping two distinct GW182 contact sites on PABPC1—a PAM2 motif binding the MLLE domain and an M2 region contacting N-terminal RRMs—defined the bipartite code through which PAM2-containing partners access PABPC1, with a single PAM2 point mutation abolishing silencing.

    Evidence Point mutagenesis of PAM2, co-IP, silencing reporters in Drosophila and human cells

    PMID:21063388

    Open questions at the time
    • Whether both binding sites must be simultaneously occupied for silencing was not resolved
  6. 2011 High

    Co-precipitation of PABPC1 with eIF4G, eIF3, and eRF3 at AUG-proximal PTCs linked translation initiation to termination and explained why AUG-proximal nonsense codons resist NMD—the closed-loop via PABPC1 makes the stop codon 'appear normal.'

    Evidence Co-IP of multiple initiation/termination factors and NMD reporter assays in human cells

    PMID:21989405

    Open questions at the time
    • Direct structural evidence for a simultaneous eIF3–eRF3–PABPC1 ternary complex lacking
  7. 2011 High

    UV-induced nuclear relocalization of PABPC1 was traced to a block in mRNA export rather than apoptotic signals, establishing that PABPC1 normally piggybacks on mRNA for nuclear exit and defaults to nuclear residence when export is inhibited.

    Evidence Live-cell imaging, subcellular fractionation, mRNA export inhibitors in mammalian cells

    PMID:21940797

    Open questions at the time
    • Nuclear function of accumulated PABPC1 under stress not characterized
    • Whether nuclear PABPC1 affects transcription or splicing not tested
  8. 2013 High

    Phosphorylation within intrinsically disordered regions flanking PAM2 motifs of Tob2, Pan3, and Tnrc6c regulates their binding to PABPC1, establishing a phosphorylation-dependent rheostat controlling which partners engage PABPC1 and thereby directing mRNA fate decisions.

    Evidence Phosphomimetic/phosphoblocking mutants, co-IP, mRNA half-life and silencing assays

    PMID:23340509

    Open questions at the time
    • Kinases responsible for these phosphorylation events not identified
    • Whether phosphorylation of PABPC1 itself synergizes with partner phosphorylation unknown
  9. 2016 High

    Reconstitution of BTG2-stimulated CAF1 deadenylase activity with purified PABPC1 RRM1 and BTG2 APRO domain demonstrated that PABPC1 is not merely a passive poly(A) anchor but an active co-stimulator of deadenylation through a specific protein–protein interface.

    Evidence In vitro reconstituted deadenylase assay, domain mapping, cell proliferation assays

    PMID:26912148

    Open questions at the time
    • How BTG2-PABPC1 complex hands off poly(A) to CAF1 active site was structurally unresolved at this stage
  10. 2017 High

    Poly(A) tail length on PABPC1's own mRNA controls its protein output in cardiomyocytes, revealing an autoregulatory feedback loop that titrates cellular translation capacity during hypertrophic growth.

    Evidence Poly(A) tail length sequencing, ribosome profiling, cardiac gain/loss-of-function mouse models

    PMID:28653618

    Open questions at the time
    • Factors that shorten or lengthen PABPC1 mRNA poly(A) tail in this circuit not identified
    • Generalizability beyond cardiomyocytes not tested
  11. 2020 High

    Targeting PABPC1 to mitochondria demonstrated that poly(A)-binding alone is sufficient to block mitochondrial translation, clarifying that PABPC1 is excluded from mitochondria for functional reasons and that ectopic poly(A) coating can dominantly inhibit organellar protein synthesis.

    Evidence Mitochondrial targeting construct, metabolic labeling of mitochondrial translation

    PMID:20144953

    Open questions at the time
    • Mechanism of translational block (ribosome loading vs. elongation) not distinguished
  12. 2020 High

    LARP1 requires its PAM2 motif–PABPC1 interaction for mRNA stabilization and poly(A) length protection, positioning PABPC1 as a recruitment platform through which LARP1 accesses target mRNAs for mTOR-dependent translational regulation.

    Evidence PAM2 point mutagenesis, co-IP, poly(A) length and mRNA stability assays

    PMID:33292040 PMID:33332560

    Open questions at the time
    • Whether mTOR directly phosphorylates the LARP1–PABPC1 interface not shown
    • Structural basis of LARP1–PABPC1 ternary complex with mRNA unknown
  13. 2021 High

    MKRN3-mediated K27/K29-linked ubiquitination of PABPC1 was non-proteolytic and suppressed global translation, establishing ubiquitin chain editing of PABPC1 as a regulatory mechanism linking an E3 ligase with tumor-suppressor function to translation control.

    Evidence MS-based substrate discovery, in vitro/in vivo ubiquitination assays, E3-dead mutant, mouse lung cancer models

    PMID:34143182

    Open questions at the time
    • Specific lysine residues on PABPC1 modified by MKRN3 not mapped
    • Mechanism by which K27/K29-Ub chains reduce translation not elucidated
  14. 2021 High

    NMR-guided mutagenesis of the BTG1/BTG2 boxC motif and PABPC1 RRM1 defined the molecular interface at atomic resolution and confirmed it is necessary for deadenylation, closing a structural gap from the 2016 reconstitution.

    Evidence NMR chemical shift perturbation, mutagenesis, in vitro and in cellulo deadenylation assays

    PMID:34060423

    Open questions at the time
    • Full quaternary complex structure at high resolution not yet available
  15. 2022 High

    NMR-guided docking produced a quaternary poly(A)–PABPC1–BTG2–CAF1 model showing that BTG2 positions the poly(A) 3' end toward the CAF1 catalytic center, providing the first structural explanation for how PABPC1–BTG2 coupling stimulates deadenylation.

    Evidence NMR-guided molecular docking, mutagenesis/pulldown validation

    PMID:35640718

    Open questions at the time
    • Model is docking-based; high-resolution cryo-EM or crystallographic validation lacking
  16. 2022 High

    Biophysical dissection showed Paip2A displaces PABPC1 from poly(A) by sequentially engaging RRM2 then RRM3 at the same surfaces used for poly(A) recognition, establishing the competitive inhibition mechanism that limits PABPC1-dependent translation.

    Evidence ITC, NMR of interaction interfaces, in vitro competition assays

    PMID:35307347

    Open questions at the time
    • In vivo dynamics of Paip2A–PABPC1 competition on actively translating polysomes not measured
  17. 2022 High

    De novo PABPC1 missense variants that weaken PAIP2 binding and impair neural progenitor proliferation in utero linked PABPC1 to a neurodevelopmental disorder, demonstrating that dosage or activity perturbation of PABPC1 is not tolerated during cortical neurogenesis.

    Evidence Exome sequencing, co-IP of mutant PABPC1 with PAIP2, in utero electroporation rescue in mouse

    PMID:35511136

    Open questions at the time
    • Whether the disease mechanism is translational or involves altered mRNA decay not distinguished
    • Number of affected families small
  18. 2023 High

    USP10 was identified as the deubiquitinase that reverses MKRN3-type K27/K29-linked ubiquitination on PABPC1 RRM2, restoring efficient eIF4G binding and mRNA translation—completing the ubiquitin editing cycle that tunes PABPC1 translation activity.

    Evidence In vitro deubiquitination assay, mRNA-RIP, polysome profiling, PDAC xenograft model

    PMID:37757903

    Open questions at the time
    • Whether MKRN3 and USP10 act on the same pool of PABPC1 in the same cell type not tested
    • Ubiquitination site identity on RRM2 not resolved to specific lysines
  19. 2024 High

    Nuclear PABPC1 cooperates with TPR to retain adenosine-rich circRNAs in the nucleus of stem cells; its decrease during neuronal differentiation licenses circRNA export required for neurite outgrowth, revealing a non-canonical nuclear RNA-sorting function for PABPC1.

    Evidence Subcellular fractionation, RIP, circRNA mutagenesis, PABPC1 knockdown during neuronal differentiation

    PMID:38838666

    Open questions at the time
    • How PABPC1 nuclear levels are regulated during differentiation not defined
    • Whether this circRNA retention applies broadly across cell types unknown
  20. 2025 High

    PABPC1 forms phase-separated condensates that selectively enhance translation of mRNAs with long structured 5' UTRs, including leukaemogenic transcripts in CML blast crisis, establishing PABPC1 condensation as a disease-relevant translation amplification mechanism.

    Evidence CRISPR screen, phase separation assays, ribosome profiling, murine/human CML xenograft models

    PMID:40102686

    Open questions at the time
    • Molecular determinants within PABPC1 that drive phase separation not identified
    • Whether condensate formation is constitutive or signal-induced not resolved
  21. 2025 High

    Stress-induced SUMOylation of PABPC1 creates a PABPC1–SUMO–TIA1 complex that selectively recruits U-rich mRNAs (including mitophagy genes) into stress granules, revealing a post-translational switch that reprograms PABPC1 from general translation factor to stress-specific mRNA triage coordinator.

    Evidence SUMOylation assay, transcriptome-wide RNA binding, co-IP of PABPC1–SUMO–TIA1, SG and mitophagy assays

    PMID:40774970

    Open questions at the time
    • SUMO E3 ligase and specific SUMO sites on PABPC1 not identified
    • Whether SUMOylation and ubiquitination compete or coexist on the same PABPC1 molecule unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: (1) how ubiquitination, SUMOylation, methylation, and phosphorylation of PABPC1 are coordinated on the same molecule; (2) whether phase-separated PABPC1 condensates represent a general translational mechanism or are restricted to specific disease contexts; and (3) the structural basis of the full-length PABPC1 in complex with eIF4G and poly(A) RNA.
  • No integrative PTM crosstalk study on PABPC1
  • No high-resolution full-length PABPC1–eIF4G–poly(A) structure
  • In vivo relevance of PABPC1 condensates outside cancer not tested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 8 GO:0060090 molecular adaptor activity 5
Localization
GO:0005829 cytosol 4 GO:0005634 nucleus 3 GO:0031410 cytoplasmic vesicle 2
Pathway
R-HSA-392499 Metabolism of proteins 6 R-HSA-8953854 Metabolism of RNA 5 R-HSA-1643685 Disease 2 R-HSA-74160 Gene expression (Transcription) 2
Partners
BTG2DDX3XEIF4G1LARP1MKRN3PAIP2TIA1USP10
Complex memberships
GW182/TNRC6–PABPC1 miRNA silencing complexPABPC1–BTG2–CAF1/CCR4-NOT deadenylation complexeIF4F–PABPC1 closed-loop complex

Evidence

Reading pass · 30 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 PABPC1 (PABP1) is a substrate of the arginine methyltransferase CARM1; the methylated region was mapped in vitro and methylation was confirmed in vivo using high-density protein array screens. High-density protein array solid-phase enzyme assay, in vitro methylation mapping, in vivo methylation confirmation EMBO reports High 11850402
2007 PABPC1 position relative to the poly(A) tail is a critical determinant for PTC definition in NMD: tethering PABPC1 downstream of a PTC abolishes NMD, and depletion of PABPC1 suppresses NMD independently of translation efficiency changes. Tethering assay (PABPC1 downstream of PTC), RNAi knockdown, reporter mRNA assays in Drosophila The EMBO journal High 17318186
2009 The GW182 silencing domain competes with eIF4G for binding to PABPC1; GW182-PABPC1 interaction promotes deadenylation of miRNA targets by recruiting the CCR4-NOT deadenylase complex, and PABPC1 overexpression suppresses miRNA-target silencing. Co-immunoprecipitation, competition binding assay, tethering assay, overexpression/knockdown with reporter silencing readout in Drosophila S2 cells Molecular and cellular biology High 19797087
2010 Two distinct regions of GW182 proteins interact with PABPC1: a PAM2 motif (binding PABPC1 C-terminal MLLE domain) and an M2/C-terminal region (indirect binding to PABPC1 N-terminal domain); a single amino acid substitution in the PAM2 motif abolishes both PABPC1 binding and GW182 silencing activity. Co-immunoprecipitation, point mutagenesis of PAM2 motif, functional silencing reporter assays in Drosophila and human cells The EMBO journal High 21063388
2011 PABPC1 interacts with eIF4G, eIF3, and eRF3 at AUG-proximal PTCs, linking translation initiation to efficient termination; this 3'-5' linkage underlies resistance to NMD at AUG-proximal nonsense mutations. Co-immunoprecipitation of PABPC1 with eIF4G, eIF3, eRF3; ribosome stalling assay; reporter NMD assays in human cells Nucleic acids research High 21989405
2011 UV irradiation causes nuclear relocalization of cytoplasmic PABPC1 (and PABPC4); this relocalization is mechanistically linked to a block in mRNA export rather than apoptosis, and perturbations blocking mRNA export recapitulate PABPC1 nuclear accumulation, indicating that nuclear export of PABPC1 is dependent on ongoing mRNA export. Live-cell imaging, subcellular fractionation, PABP knockdown, inhibitors of mRNA export, UV irradiation in mammalian cells Journal of cell science High 21940797
2016 BTG2's APRO domain directly binds the first RRM domain of PABPC1, and together PABPC1 RRM1 and BTG2 APRO are sufficient to stimulate CAF1 deadenylase activity in vitro; this interaction is required for BTG2 to control cell proliferation. In vitro deadenylase activity assay with purified components, co-immunoprecipitation, domain mapping, cell proliferation assay with BTG2/PABPC1 mutants Nature communications High 26912148
2003 Influenza NS1 protein interacts with PABPC1 in an RNA-independent manner in vivo and in vitro; the interaction maps to residues 365–535 of PABPC1 and residues 1–81 of NS1; NS1 does not compete with PABPC1 for eIF4GI binding, suggesting a trimeric NS1–eIF4GI–PABPC1 complex that promotes viral mRNA translation. Co-immunoprecipitation in infected cells, GST pulldown (in vitro), deletion mapping of interaction domains The Journal of general virology High 14645908
2013 Phosphorylation at serine/threonine residues within intrinsically disordered regions flanking PAM2 motifs of PABPC1-interacting proteins (Tob2, Pan3, Tnrc6c) regulates their ability to bind PABPC1 and thereby affects mRNA turnover and gene silencing. Phospho-blocking and phosphomimetic mutant analysis, co-immunoprecipitation, mRNA half-life and silencing reporter assays in mammalian cells RNA (New York, N.Y.) High 23340509
2021 MKRN3 is an E3 ubiquitin ligase that ubiquitinates PABPC1 (non-proteolytic K27/K29-linked ubiquitination); MKRN3 tumor suppressor function is dependent on its E3 ligase activity and MKRN3-mediated PABPC1 ubiquitination, which modulates global protein synthesis. MS-based proteomics screen for MKRN3 substrates, in vitro and in vivo ubiquitination assays, E3 ligase dead mutant analysis, cell proliferation assays, in vivo mouse lung cancer models The Journal of experimental medicine High 34143182
2023 USP10 deubiquitinates PABPC1 by cleaving K27/K29-linked ubiquitin chains on the RRM2 domain; deubiquitinated PABPC1 binds more efficiently to CLK2 mRNA and eIF4G1, increasing translation efficiency and promoting PDAC tumor growth. Co-immunoprecipitation, in vitro deubiquitination assay, mRNA-RIP, polysome profiling reporter, xenograft mouse model Cancer letters High 37757903
2022 Paip2A inhibits translation by binding competitively to the RRM2-RRM3 regions of PABPC1 at the same interfaces used for poly(A) binding; Paip2A initially anchors to RRM2 and then displaces RRM3 from poly(A), causing full PABPC1 dissociation from the poly(A) tail. Isothermal titration calorimetry, NMR analysis of interaction interfaces, in vitro binding competition assays The Journal of biological chemistry High 35307347
2020 Crystal structure of the PAM2w motif of LARP4B bound to the MLLE domain of PABPC1 was determined, revealing a variant PAM2-MLLE interaction mode; mutational studies in vitro and in vivo confirmed the structural interface. X-ray crystallography, mutational analysis in vitro (pulldown) and in vivo Biomolecules High 32517187
2020 Targeting PABPC1 to mitochondria (mtPABP1) coats the mitochondrial poly(A) tail without causing transcript decay but causes marked inhibition of mitochondrial translation, demonstrating that poly(A) tail binding by PABPC1 is sufficient to block mitochondrial protein synthesis. Mitochondrial targeting construct (functional gain-of-function in organello), mt-mRNA steady-state analysis, metabolic labeling of mitochondrial translation in human cells Nucleic acids research High 20144953
2017 Poly(A) tail length on the PABPC1 mRNA itself dynamically controls PABPC1 protein synthesis in cardiomyocytes, constituting a feedback regulatory mechanism that titrates global translation capacity in response to hypertrophic stimuli. Poly(A) tail length sequencing, ribosome profiling, cardiac-specific gain- and loss-of-function mouse models, hypertrophic stimulation assays eLife High 28653618
2021 PABPC1 is required for the association of LARP1 with its specific mRNA targets; PABPC1 and LARP1 share a highly overlapping mRNA interactome, and mRNAs co-bound by both proteins are translationally repressed following mTOR inhibition. RNA-binding protein mRNA interactome capture, LARP1/PABPC1 co-immunoprecipitation, mRNA translation state analysis after mTOR inhibition, PABPC1 knockdown Nucleic acids research High 33332560
2022 De novo missense variants in the PABP domain of PABPC1 weaken its interaction with PAIP2 (confirmed by co-immunoprecipitation) and impair neural progenitor cell proliferation in utero (confirmed by electroporation rescue), linking PABPC1 to cortical neurogenesis. Exome sequencing, molecular modeling, co-immunoprecipitation of mutant vs. wild-type PABPC1 with PAIP2, in utero electroporation mouse brain rescue assay Genetics in medicine High 35511136
2012 DDX3 directly interacts with PABPC1 (stress granule marker) and this interaction contributes to stress granule assembly; DDX3 knockdown causes nuclear accumulation of PABPC1 and prevents SG formation, while shRNA-resistant DDX3 restores PABPC1 cytoplasmic localization and SG assembly. Co-immunoprecipitation, siRNA knockdown, shRNA rescue, immunofluorescence localization in mammalian cells under stress The Biochemical journal Medium 21883093
2020 Seneca Valley Virus 3Cpro cleaves PABPC1 at residue 437 via its protease activity (point mutants H48A and C160A of 3Cpro abolish cleavage); PABPC1 cleavage inhibits host protein synthesis and promotes viral replication, while intact PABPC1 restricts SVV replication. Viral infection cleavage assay, overexpression of 3Cpro protease mutants, site of cleavage mapping, protein synthesis assay, viral replication assay Pathogens (Basel, Switzerland) Medium 32512928
2022 PABPC1 binds the adenosine-rich 5' UTR of influenza A viral mRNAs and enables eIF4E-independent recruitment of eIF4G, thereby making viral mRNA translation more resistant to canonical cap-dependent translation inhibition. RNA-binding assay (EMSA/filter binding), in vitro translation assay, eIF4G recruitment assay in the absence of eIF4E Journal of molecular biology Medium 35074482
2017 PABPC1 interacts with hnRNPLL in T cells and plasma cells; PABPC1 promotes hnRNPLL binding to immunoglobulin mRNA and regulates the switch from membrane IgH to secreted IgH through alternative polyadenylation. Co-immunoprecipitation of PABPC1 and hnRNPLL, RIP-seq, alternative polyadenylation analysis, knockdown with immunoglobulin isoform assay The Journal of biological chemistry Medium 28611064
2021 BTG1 and BTG2 contain a conserved boxC motif within their APRO domain that is necessary and sufficient for interaction with PABPC1 RRM1, and this interaction is required for BTG1/2-stimulated deadenylation in cellulo and in vitro; NMR and mutagenesis defined the molecular interface. NMR chemical shift perturbation, mutagenesis, pulldown assays, in vitro and in cellulo deadenylation assays RNA biology High 34060423
2022 Structural model of the BTG2-PABPC1 complex (BTG2 APRO domain binding PABPC1 RRM1) was generated by NMR chemical shift perturbation-guided docking; a quaternary poly(A)-PABPC1-BTG2-Caf1/CNOT7 model shows the 3' end of poly(A) directed toward the Caf1 catalytic center, explaining BTG2-stimulated deadenylation. NMR chemical shift perturbation, mutagenesis/pulldown validation, molecular docking, model of quaternary complex Journal of molecular biology High 35640718
2025 PABPC1 forms biomolecular condensates (phase separation) that preferentially enhance translation of leukaemogenic mRNAs with long, structured 5' UTRs in CML blast crisis; genetic or pharmacological inhibition of PABPC1 suppresses disease progression and overcomes TKI resistance. CRISPR-Cas9 high-throughput screen, phase separation assays, ribosome profiling/translation efficiency analysis, murine and human CML xenograft models Nature cell biology High 40102686
2025 Under stress, PABPC1 is SUMOylated; SUMOylated PABPC1 interacts with TIA1 to form a PABPC1-SUMO-TIA1 complex that recruits U-rich element-containing mRNAs into stress granules, stabilizing them and promoting expression of mitophagy-related genes (FUNDC1, BNIP3L) to enhance cell survival. SUMO modification assay, transcriptome-wide RNA binding analysis, co-immunoprecipitation of PABPC1-SUMO-TIA1, stress granule formation assays, mitophagy assay Nature communications High 40774970
2015 PABPC1 interacts with AGO2 in the cytoplasm of hepatocellular carcinoma cells; this interaction increases recruitment of mRNA to the RISC complex and enhances miRNA-mediated silencing efficiency. Anti-AGO2 co-immunoprecipitation, mass spectrometry identification, co-localization by immunofluorescence, functional miRNA reporter assays Cancer letters Medium 26188282
2024 PABPC1 inhibits NUMB mRNA expression by interacting with AGO2 and promoting miR-31-5p and miR-146a-5p-mediated NUMB mRNA degradation, thereby activating the NOTCH1 signaling pathway. RIP, RNA pulldown, AGO2 co-immunoprecipitation, miRNA functional assays Molecular cancer Medium 38532427
2024 In H9 stem cells, PABPC1 in the nucleus binds adenosine-rich circRNAs and cooperates with nuclear basket protein TPR to trap them and prevent their export; decreased nuclear PABPC1 during neuronal differentiation enables cytoplasmic export of (A)-rich circRNAs including circRTN4(2,3), which is required for neurite outgrowth. Subcellular fractionation, RNA immunoprecipitation, circRNA mutational analysis, loss-of-function (PABPC1 knockdown), live-cell imaging during neuronal differentiation Molecular cell High 38838666
2022 CircPTK2 binds PABPC1 and enhances PABPC1's ability to stabilize SETDB1 mRNA, promoting SETDB1 expression and SETDB1-mediated EMT in bladder cancer. circRNA pulldown assay, RNA-binding protein immunoprecipitation, mRNA stability assay, gain/loss-of-function in vitro and in vivo Cancer letters Medium 36436682
2020 The isolated La-module of LARP1 mediates poly(A) length protection and mRNA stabilization through a PAM2 motif that binds PABPC1; a single point mutation in the PAM2 motif impairs both mRNA stabilization and PABPC1 binding in vivo without affecting oligo(A) RNA binding of the isolated recombinant La-module in vitro. PAM2 point mutagenesis, co-immunoprecipitation, poly(A) length analysis, mRNA stability assay in HEK293 cells, in vitro RNA binding RNA biology High 33292040

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays. EMBO reports 204 11850402
2007 A conserved role for cytoplasmic poly(A)-binding protein 1 (PABPC1) in nonsense-mediated mRNA decay. The EMBO journal 184 17318186
2012 Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response. The Biochemical journal 177 21883093
2003 PABP1 and eIF4GI associate with influenza virus NS1 protein in viral mRNA translation initiation complexes. The Journal of general virology 140 14645908
2009 The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of microRNA targets and is required for target release. Molecular and cellular biology 137 19797087
1997 Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro. Molecular and cellular biology 118 9199303
2010 Localization to, and effects of Pbp1, Pbp4, Lsm12, Dhh1, and Pab1 on stress granules in Saccharomyces cerevisiae. PloS one 108 20368989
2005 Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export. RNA (New York, N.Y.) 108 15769879
2022 CircPTK2/PABPC1/SETDB1 axis promotes EMT-mediated tumor metastasis and gemcitabine resistance in bladder cancer. Cancer letters 99 36436682
2018 Long non-coding RNA SNHG14 induces trastuzumab resistance of breast cancer via regulating PABPC1 expression through H3K27 acetylation. Journal of cellular and molecular medicine 94 30063126
2005 Yeast poly(A)-binding protein, Pab1, and PAN, a poly(A) nuclease complex recruited by Pab1, connect mRNA biogenesis to export. Genes & development 92 15630021
2011 Interaction of PABPC1 with the translation initiation complex is critical to the NMD resistance of AUG-proximal nonsense mutations. Nucleic acids research 84 21989405
2010 Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated gene silencing. The EMBO journal 83 21063388
2022 PABPC1-induced stabilization of IFI27 mRNA promotes angiogenesis and malignant progression in esophageal squamous cell carcinoma through exosomal miRNA-21-5p. Journal of experimental & clinical cancer research : CR 79 35346324
2012 ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice. PLoS genetics 75 22956915
2010 Role of GW182 proteins and PABPC1 in the miRNA pathway: a sense of déjà vu. Nature reviews. Molecular cell biology 73 20379206
2017 Poly(A) tail length regulates PABPC1 expression to tune translation in the heart. eLife 72 28653618
2016 BTG2 bridges PABPC1 RNA-binding domains and CAF1 deadenylase to control cell proliferation. Nature communications 68 26912148
2011 Nuclear relocalisation of cytoplasmic poly(A)-binding proteins PABP1 and PABP4 in response to UV irradiation reveals mRNA-dependent export of metazoan PABPs. Journal of cell science 67 21940797
2011 Stability of a long noncoding viral RNA depends on a 9-nt core element at the RNA 5' end to interact with viral ORF57 and cellular PABPC1. International journal of biological sciences 63 22043172
2018 circRNA of AR-suppressed PABPC1 91 bp enhances the cytotoxicity of natural killer cells against hepatocellular carcinoma via upregulating UL16 binding protein 1. Oncology letters 62 30655779
2001 The yeast hsp70 homologue Ssa is required for translation and interacts with Sis1 and Pab1 on translating ribosomes. The Journal of biological chemistry 61 11279042
2020 PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay. Cell death & disease 57 32015336
2015 PABPC1 interacts with AGO2 and is responsible for the microRNA mediated gene silencing in high grade hepatocellular carcinoma. Cancer letters 57 26188282
2008 Nonsense-mediated mRNA decay in yeast does not require PAB1 or a poly(A) tail. Molecular cell 54 18206975
2022 PABPC1--mRNA stability, protein translation and tumorigenesis. Frontiers in oncology 50 36531055
2000 Deletion of the PAT1 gene affects translation initiation and suppresses a PAB1 gene deletion in yeast. Molecular and cellular biology 49 10779343
2020 LncRNA SNHG14 promotes hepatocellular carcinoma progression via H3K27 acetylation activated PABPC1 by PTEN signaling. Cell death & disease 47 32811821
2024 Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway. Molecular cancer 46 38532427
2021 E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer. The Journal of experimental medicine 44 34143182
2012 The Caenorhabditis elegans GW182 protein AIN-1 interacts with PAB-1 and subunits of the PAN2-PAN3 and CCR4-NOT deadenylase complexes. Nucleic acids research 44 22402495
2007 PAB1 self-association precludes its binding to poly(A), thereby accelerating CCR4 deadenylation in vivo. Molecular and cellular biology 41 17620415
2013 Phosphorylation at intrinsically disordered regions of PAM2 motif-containing proteins modulates their interactions with PABPC1 and influences mRNA fate. RNA (New York, N.Y.) 40 23340509
2010 Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes mitochondrial translation inhibition. Nucleic acids research 40 20144953
2010 PUF3 acceleration of deadenylation in vivo can operate independently of CCR4 activity, possibly involving effects on the PAB1-mRNP structure. Journal of molecular biology 40 20435044
2014 Interaction between the poly(A)-binding protein Pab1 and the eukaryotic release factor eRF3 regulates translation termination but not mRNA decay in Saccharomyces cerevisiae. RNA (New York, N.Y.) 36 25411355
2015 PABPC1 exerts carcinogenesis in gastric carcinoma by targeting miR-34c. International journal of clinical and experimental pathology 33 26097561
2012 Epab and Pabpc1 are differentially expressed during male germ cell development. Reproductive sciences (Thousand Oaks, Calif.) 33 22814100
2021 The mTOR regulated RNA-binding protein LARP1 requires PABPC1 for guided mRNA interaction. Nucleic acids research 32 33332560
2018 Extensive Structural Differences of Closely Related 3' mRNA Isoforms: Links to Pab1 Binding and mRNA Stability. Molecular cell 30 30318446
1999 Decapping of stabilized, polyadenylated mRNA in yeast pab1 mutants. Yeast (Chichester, England) 30 10392446
2020 The isolated La-module of LARP1 mediates 3' poly(A) protection and mRNA stabilization, dependent on its intrinsic PAM2 binding to PABPC1. RNA biology 29 33292040
2018 The Saccharomyces cerevisiae poly (A) binding protein (Pab1): Master regulator of mRNA metabolism and cell physiology. Yeast (Chichester, England) 27 30006991
2017 Cytoplasmic poly(A)-binding protein 1 (PABPC1) interacts with the RNA-binding protein hnRNPLL and thereby regulates immunoglobulin secretion in plasma cells. The Journal of biological chemistry 27 28611064
2015 Arabidopsis pab1, a mutant with reduced anthocyanins in immature seeds from banyuls, harbors a mutation in the MATE transporter FFT. Plant molecular biology 26 26608698
2023 Deubiquitinating PABPC1 by USP10 upregulates CLK2 translation to promote tumor progression in pancreatic ductal adenocarcinoma. Cancer letters 25 37757903
2019 Transthyretin Upregulates Long Non-Coding RNA MEG3 by Affecting PABPC1 in Diabetic Retinopathy. International journal of molecular sciences 25 31847264
2010 Antagonistic roles of PP2A-Pab1 and Etd1 in the control of cytokinesis in fission yeast. Genetics 25 20876564
1997 The wheat poly(A)-binding protein functionally complements pab1 in yeast. European journal of biochemistry 23 9030759
2020 Seneca Valley Virus 3Cpro Cleaves PABPC1 to Promote Viral Replication. Pathogens (Basel, Switzerland) 21 32512928
2017 The helicase, DDX3X, interacts with poly(A)-binding protein 1 (PABP1) and caprin-1 at the leading edge of migrating fibroblasts and is required for efficient cell spreading. The Biochemical journal 21 28733330
2023 The HOXD9-mediated PAXIP1-AS1 regulates gastric cancer progression through PABPC1/PAK1 modulation. Cell death & disease 20 37225681
2021 Functional implications of PABPC1 in the development of ovarian cancer. Open medicine (Warsaw, Poland) 19 34027108
2016 The poly(A)-binding protein genes, EPAB, PABPC1, and PABPC3 are differentially expressed in infertile men with non-obstructive azoospermia. Journal of assisted reproduction and genetics 19 26843391
2002 The pab1 gene of Coprinus cinereus encodes a bifunctional protein for para-aminobenzoic acid (PABA) synthesis: implications for the evolution of fused PABA synthases. Journal of basic microbiology 19 11981873
2001 The chromosomal region containing pab-1, mip, and the A mating type locus of the secondarily homothallic homobasidiomycete Coprinus bilanatus. Current genetics 19 11318102
2024 Helicobacter Pylori-Enhanced hnRNPA2B1 Coordinates with PABPC1 to Promote Non-m6A Translation and Gastric Cancer Progression. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 18 38887155
2023 LncRNA WDR11-AS1 Promotes Extracellular Matrix Synthesis in Osteoarthritis by Directly Interacting with RNA-Binding Protein PABPC1 to Stabilize SOX9 Expression. International journal of molecular sciences 18 36614257
2014 Epab and Pabpc1 are differentially expressed in the postnatal mouse ovaries. Journal of assisted reproduction and genetics 18 25370180
2023 PEDV N protein capture protein translation element PABPC1 and eIF4F to promote viral replication. Veterinary microbiology 17 37572396
2024 Altered nucleocytoplasmic export of adenosine-rich circRNAs by PABPC1 contributes to neuronal function. Molecular cell 16 38838666
2015 The Saccharomyces cerevisiae poly(A) binding protein Pab1 as a target for eliciting stress tolerant phenotypes. Scientific reports 16 26658950
2018 Phosphorylation and interactions associated with the control of the Leishmania Poly-A Binding Protein 1 (PABP1) function during translation initiation. RNA biology 15 29569995
2017 Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner. RNA (New York, N.Y.) 15 28986506
2014 Only a subset of the PAB1-mRNP proteome is present in mRNA translation complexes. Protein science : a publication of the Protein Society 15 24838188
2024 LncRNA PRBC induces autophagy to promote breast cancer progression through modulating PABPC1-mediated mRNA stabilization. Oncogene 14 38366145
2023 PABPC1 promotes cell proliferation and metastasis in pancreatic adenocarcinoma by regulating COL12A1 expression. Immunity, inflammation and disease 14 37506150
2022 PABP1 Drives the Selective Translation of Influenza A Virus mRNA. Journal of molecular biology 14 35074482
2016 Functional compensation for the loss of testis-specific poly(A)-binding protein, PABPC2, during mouse spermatogenesis. The Journal of reproduction and development 14 26971890
2014 The involvement of mRNA processing factors TIA-1, TIAR, and PABP-1 during mammalian hibernation. Cell stress & chaperones 13 24590458
2008 Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNA. Retrovirology 13 18957126
2013 PAB-1, a Caenorhabditis elegans poly(A)-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1. PloS one 12 24367695
2021 A conserved motif in human BTG1 and BTG2 proteins mediates interaction with the poly(A) binding protein PABPC1 to stimulate mRNA deadenylation. RNA biology 11 34060423
2021 Identification of novel proteins and mRNAs differentially bound to the Leishmania Poly(A) Binding Proteins reveals a direct association between PABP1, the RNA-binding protein RBP23 and mRNAs encoding ribosomal proteins. PLoS neglected tropical diseases 11 34705820
2020 Crystal Structure of a Variant PAM2 Motif of LARP4B Bound to the MLLE Domain of PABPC1. Biomolecules 10 32517187
2019 The Leishmania PABP1-eIF4E4 interface: a novel 5'-3' interaction architecture for trans-spliced mRNAs. Nucleic acids research 10 30476241
2018 Domain-functional analyses of PIWIL1 and PABPC1 indicate their synergistic roles in protein translation via 3'-UTRs of meiotic mRNAs. Biology of reproduction 10 29701755
2017 Express yourself: how PP2A-B55Pab1 helps TORC1 talk to TORC2. Current genetics 10 28643116
2011 The stress granule protein Vgl1 and poly(A)-binding protein Pab1 are required for doxorubicin resistance in the fission yeast Schizosaccharomyces pombe. Biochemical and biophysical research communications 10 22172946
2000 The yeast polyadenylate-binding protein (PAB1) gene acts as a disease lesion mimic gene when expressed in plants. Plant molecular biology 9 10794533
1999 The promoter of the Poly(A) binding protein 2 (Pabp2) retroposon is derived from the 5'-untranslated region of the Pabp1 progenitor gene. Genomics 9 10534404
2022 Paip2A inhibits translation by competitively binding to the RNA recognition motifs of PABPC1 and promoting its dissociation from the poly(A) tail. The Journal of biological chemistry 8 35307347
2022 Pab1 acetylation at K131 decreases stress granule formation in Saccharomyces cerevisiae. The Journal of biological chemistry 8 36572187
2021 Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 8 33470388
2020 Chromosome-level de novo assembly of Coprinopsis cinerea A43mut B43mut pab1-1 #326 and genetic variant identification of mutants using Nanopore MinION sequencing. Fungal genetics and biology : FG & B 8 33253902
2018 RNA Modulates the Interaction between Influenza A Virus NS1 and Human PABP1. Biochemistry 8 29782795
2010 Two mutations in pab-1 encoding poly(A)-binding protein show similar defects in germline stem cell proliferation but different longevity in C. elegans. Molecules and cells 8 20680489
2025 PABPC1 SUMOylation enhances cell survival by promoting mitophagy through stabilizing U-rich mRNAs within stress granules. Nature communications 7 40774970
2024 Atherosclerosis-related biomarker PABPC1 predicts pan-cancer events. Stroke and vascular neurology 7 37311641
2022 Structural Model of the Human BTG2-PABPC1 Complex by Combining Mutagenesis, NMR Chemical Shift Perturbation Data and Molecular Docking. Journal of molecular biology 7 35640718
2019 Messenger RNAs of Yeast Virus-Like Elements Contain Non-templated 5' Poly(A) Leaders, and Their Expression Is Independent of eIF4E and Pab1. Frontiers in microbiology 7 31736885
2025 Selective translational control by PABPC1 phase separation regulates blast crisis and therapy resistance in chronic myeloid leukaemia. Nature cell biology 6 40102686
2025 EV-D68 cleaves LARP1 and PABPC1 by 3Cpro to redirect host mRNA translation machinery toward its genomic RNA. PLoS pathogens 6 40294010
2024 Stable structures or PABP1 loading protects cellular and viral RNAs against ISG20-mediated decay. Life science alliance 6 38418089
2024 Circ_PABPC1 promotes the malignancy of gastric cancer through interacting with ILK to activate NF-κB pathway. Experimental cell research 6 38688434
2024 LINC01197 inhibits influenza A virus replication by serving as a PABPC1 decoy. Veterinary research 6 39334466
2022 De novo variants in the PABP domain of PABPC1 lead to developmental delay. Genetics in medicine : official journal of the American College of Medical Genetics 6 35511136
2020 Influenza A Virus NS1 Protein Binds as a Dimer to RNA-Free PABP1 but Not to the PABP1·Poly(A) RNA Complex. Biochemistry 6 33172261
2019 Amorphous silica nanoparticles induce tumorigenesis via regulating ATP5H/SOD1-related oxidative stress, oxidative phosphorylation and EIF4G2/PABPC1-associated translational initiation. PeerJ 6 30863671
1993 Localization of the human poly(A)-binding protein gene (PAB1) to chromosomal regions 3q22-q25, 12q13-q14, and 13q12-q13 by in situ hybridization. Genomics 6 8432538