Affinage

NUSAP1

Nucleolar and spindle-associated protein 1 · UniProt Q9BXS6

Length
441 aa
Mass
49.5 kDa
Annotated
2026-06-10
65 papers in source corpus 26 papers cited in narrative 26 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NUSAP1 is a microtubule-associated protein that localizes to dynamic spindle microtubules in a chromosome-centric pattern during metaphase and anaphase, where it organizes spindle architecture and chromosome segregation through SUMO-pathway and kinesin interactions [PMID:28900032, PMID:bio_10.1101_2025.05.16.654427]. Through its SAP domain it engages the RanBP2-RanGAP1-UBC9 SUMO E3 ligase complex, and two consensus SUMOylation sites within its DNA- and microtubule-binding domains are modified in a RANBP2-dependent manner, each site selectively controlling NUSAP1 localization and dictating distinct mitotic outcomes (PMID:28900032, PMID:37947624). NUSAP1 directly binds KIF2C and Aurora kinase A; AURKA-mediated phosphorylation tunes NUSAP1-KIF2C affinity to balance spindle microtubule stability against depolymerization, and truncating mutations (Y403*, Q405*) that disrupt this interaction abolish its rescue activity [PMID:bio_10.1101_2025.05.16.654427]. Beyond mitosis, NUSAP1 supports genome stability: it sustains BRCA1 protein levels and BRCA1 focus formation to enable homologous-recombination and single-strand-annealing repair and proper centrosome duplication (PMID:24521615), and it acts upstream of the RNA-binding protein ILF2 (also interacting with DHX9) to suppress R-loop accumulation and DNA damage (PMID:37047232). Across cancers, NUSAP1 is deployed as an oncogenic protein-stabilizing and transcription-regulating hub—forming a c-Myc/HIF-1α complex at the LDHA promoter to drive glycolysis (PMID:37354982), stabilizing partner proteins including ATR, YAP1, ANXA2 and Notch2 by antagonizing their ubiquitin-dependent degradation (PMID:32317623, PMID:33489890, PMID:39212774, PMID:40393971), and is itself transcriptionally controlled by the RB1/E2F1 and E2F8 axes (PMID:25585568, PMID:37331996). In triple-negative breast cancer, by contrast, NUSAP1 acts as a tumor suppressor, recruiting DAXX via its microtubule-associated domain to bridge HIFα and deposit SETDB1-dependent H3K9me3 marks on hypoxia response elements to repress HIF target genes (PMID:41178464). A recurrent de novo nonsense variant (p.Tyr403Ter) that escapes nonsense-mediated decay causes microcephaly, severe developmental delay, and epilepsy (PMID:37005340), consistent with conserved developmental roles seen in zebrafish neural crest migration (PMID:21203972) and mouse oocyte spindle assembly (PMID:37992207).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 2014 Medium

    Established that NUSAP1 contributes to genome maintenance beyond its mitotic role by linking it to double-strand break repair and centrosome integrity.

    Evidence siRNA depletion with HR/SSA repair assays, BRCA1 focus imaging, and plasmid rescue in human cells

    PMID:24521615

    Open questions at the time
    • Mechanism by which NUSAP1 controls BRCA1 protein level not defined
    • No direct NUSAP1-BRCA1 binding shown
  2. 2015 Medium

    Placed NUSAP1 transcription under the RB1/E2F1 axis, explaining its cell-cycle-coupled expression and elevation in RB1-deficient cancer.

    Evidence Lentiviral RB1 and siRNA E2F1 knockdown with RT-qPCR, Western blot, and proliferation/invasion assays in prostate cancer cells

    PMID:25585568

    Open questions at the time
    • Direct E2F1 binding to the NUSAP1 promoter not demonstrated here
    • Does not address post-translational regulation
  3. 2017 High

    Identified the molecular basis of NUSAP1's mitotic function as a SUMO-pathway cofactor by mapping its interaction with the RanBP2-RanGAP1-UBC9 complex.

    Evidence Endogenous reciprocal Co-IP with mass spectrometry, live-cell imaging, and Taxol-sensitivity phenotyping in human cells

    PMID:28900032

    Open questions at the time
    • Specific SUMO substrates recognized via the SAP domain not identified
    • Single lab
  4. 2019 Medium

    Demonstrated an in vivo developmental requirement for NUSAP1 in cell migration and neural crest morphogenesis.

    Evidence Zebrafish morpholino knockdown with neural crest marker in situ hybridization and mRNA rescue

    PMID:21203972

    Open questions at the time
    • Molecular mechanism linking NUSAP1 to neural crest migration unresolved
    • Morpholino specificity limitations
  5. 2020 Medium

    Provided a substrate-level mechanism for SAP-domain function by showing NUSAP1 promotes ATR sumoylation to antagonize its proteolysis and confer chemoresistance.

    Evidence Co-IP, sumoylation and ubiquitination assays, SAP-domain mutation, and viability assays in glioblastoma cells

    PMID:32317623

    Open questions at the time
    • Direct SUMO E3 activity vs cofactor role not separated
    • Single lab
  6. 2021 Medium

    Extended NUSAP1's protein-stabilizing role to cancer signaling hubs YAP1 and cell-cycle regulators CDC20/CCNA2 driving proliferation.

    Evidence Co-IP, siRNA knockdown, epistasis rescue, and xenografts in gastric cancer and osteosarcoma cells

    PMID:33489890 PMID:34079289

    Open questions at the time
    • Whether NUSAP1 stabilizes YAP1 by blocking ubiquitination not directly tested
    • Direct binding surfaces undefined
  7. 2022 Medium

    Mapped additional transcriptional inputs (HOXB2) and downstream effectors (TOP2A) that integrate NUSAP1 into proliferative cancer programs.

    Evidence ChIP and dual-luciferase reporter for HOXB2, RNA-seq plus double knockdown for TOP2A, with xenograft models

    PMID:35485274 PMID:35532155

    Open questions at the time
    • TOP2A regulation shown without direct binding
    • Tissue specificity of these axes unclear
  8. 2023 High

    Resolved the site-specific logic of NUSAP1 SUMOylation, showing two RANBP2-dependent sites independently govern localization and daughter-cell fate.

    Evidence Site-directed mutagenesis, proximity ligation with SUMO2/3, and mitotic phenotyping in human cells

    PMID:37947624

    Open questions at the time
    • Downstream effectors of each modified site not identified
  9. 2023 High

    Connected NUSAP1 to RNA metabolism and R-loop suppression by placing it upstream of ILF2/DHX9 in the DNA damage response.

    Evidence AP-MS interactome, reciprocal Co-IP, confocal colocalization, domain mapping, and R-loop/DNA damage assays after camptothecin

    PMID:37047232

    Open questions at the time
    • How NUSAP1 mechanistically enables ILF2 R-loop suppression unclear
    • Single lab
  10. 2023 Medium

    Defined a metabolic-transcriptional role in which NUSAP1 joins c-Myc and HIF-1α at the LDHA promoter, reinforced by lactylation-driven stabilization.

    Evidence ChIP-seq, Co-IP, RNA-seq, single-cell RNA-seq, and xenografts

    PMID:37354982

    Open questions at the time
    • Lactylation site on NUSAP1 not mapped
    • Direct DNA contact by NUSAP1 vs partners unclear
  11. 2023 Medium

    Identified E2F8 as an additional direct transcriptional activator of NUSAP1 mediating cisplatin resistance.

    Evidence ChIP, dual-luciferase reporter, knockdown, comet assay, and γ-H2AX analysis in hepatocellular carcinoma

    PMID:37331996

    Open questions at the time
    • Relationship to RB1/E2F1 axis not integrated
  12. 2023 Medium

    Confirmed conserved meiotic spindle and RNA-associated functions in mammalian oocytes, reinforcing the link between NUSAP1 and RNA-binding partners.

    Evidence Proteomics, siRNA depletion, immunofluorescence localization, aneuploidy scoring, and MS interactome in mouse oocytes

    PMID:37992207

    Open questions at the time
    • Direct role in P-body dynamics inferred, not demonstrated
    • Specific RNA targets unidentified
  13. 2023 Medium

    Linked NUSAP1 to human neurodevelopmental disease through a recurrent de novo nonsense variant acting via a non-haploinsufficiency mechanism.

    Evidence Exome sequencing, NMD escape transcript assay, and single-cell RNA-seq of post-mortem brain

    PMID:37005340

    Open questions at the time
    • Dominant-negative vs gain-of-function mechanism unresolved
    • Single family/lab cohort
  14. 2024 Medium

    Extended the protein-stabilization paradigm to ANXA2, tying NUSAP1 to radioresistance via DNA-damage repair.

    Evidence IP-MS, ubiquitination assay, comet assay, and xenografts in gastric cancer

    PMID:39212774

    Open questions at the time
    • E3 ligase antagonized by NUSAP1 not identified
  15. 2025 Medium

    Established the direct AURKA-NUSAP1-KIF2C axis that balances spindle microtubule dynamics and tied disease-associated truncations to its disruption.

    Evidence Co-IP, phosphorylation assay, NUSAP1-KO rescue, mouse microcephaly model, and live imaging (preprint)

    PMID:bio_10.1101_2025.05.16.654427

    Open questions at the time
    • Preprint, single lab
    • Phosphosites on NUSAP1 not fully mapped
  16. 2025 Medium

    Revealed a context-dependent tumor-suppressor function in which NUSAP1 represses HIF transcriptional output via DAXX/SETDB1-mediated H3K9me3 deposition.

    Evidence Co-IP of NUSAP1-DAXX-HIFα, ChIP for H3K9me3 at HREs, and MAD-domain rescue in triple-negative breast cancer

    PMID:41178464

    Open questions at the time
    • Determinants of oncogenic vs tumor-suppressor switching unknown
    • Single lab
  17. 2025 Medium

    Added a PRMT1-dependent methylation layer (R418/R422) enabling NUSAP1 to stabilize Notch2 and drive 5-FU resistance.

    Evidence Proteomics, site-directed mutagenesis, Co-IP, and ubiquitination assays in gastric cancer

    PMID:40393971

    Open questions at the time
    • Structural basis of methyl-dependent PEST-domain binding undefined

Open questions

Synthesis pass · forward-looking unresolved questions
  • What governs the switch between NUSAP1's oncogenic protein-stabilizing/transcriptional roles and its tumor-suppressive HIF-repressive role, and how its mitotic, DNA-repair, and disease-causing functions are mechanistically unified, remains unresolved.
  • No unifying biochemical model across contexts
  • Structural basis of SAP/MAD domain partner selection unknown
  • Direct enzymatic vs adaptor role in SUMO and ubiquitin pathways not separated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0140096 catalytic activity, acting on a protein 3 GO:0008092 cytoskeletal protein binding 2 GO:0140110 transcription regulator activity 2 GO:0003677 DNA binding 1
Localization
GO:0005634 nucleus 2 GO:0005856 cytoskeleton 2 GO:0005694 chromosome 1
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-392499 Metabolism of proteins 4 R-HSA-73894 DNA Repair 3 R-HSA-74160 Gene expression (Transcription) 2 R-HSA-1643685 Disease 1
Partners
ANXA2AURKADAXXDHX9ILF2KIF2CRANBP2YAP1
Complex memberships
NUSAP1-DAXX-HIFα repressive complexRanBP2-RanGAP1-UBC9 SUMO E3 ligase complexc-Myc/HIF-1α/NUSAP1 transcriptional complex at LDHA promoter

Evidence

Reading pass · 26 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2017 NUSAP1 localizes to dynamic spindle microtubules in a chromosome-centric pattern near overlapping microtubules during metaphase and anaphase. Mass spectrometry identified a cell cycle-regulated interaction between NUSAP1 and the RanBP2-RanGAP1-UBC9 SUMO E3 ligase complex. The SAP domain of NUSAP1 is implicated in substrate recognition consistent with a co-factor role in sumoylation during chromosome segregation. Endogenous Co-IP, mass spectrometry, live cell imaging, siRNA knockdown with Taxol-sensitivity phenotype The Journal of biological chemistry High 28900032
2023 NUSAP1 contains two consensus SUMOylation sites within its DNA-binding and microtubule-binding domains, respectively. Site-specific mutagenesis revealed that each site selectively controls NUSAP1 localization and generates distinct mitotic defects and daughter-cell fates. NUSAP1 forms proximity ligation products with SUMO2/3 in a RANBP2-dependent manner at key mitotic sites. Site-directed mutagenesis, proximity ligation assay, cell line expression of NuSAP1 mutants, mitotic phenotyping Cells High 37947624
2020 NUSAP1 stabilizes ATR protein by promoting its sumoylation via the SAP domain, antagonizing ubiquitin-dependent proteolysis of ATR. The SAP domain of NUSAP1 was necessary for this sumoylation and for chemoresistance to TMZ and DOX in glioblastoma cells. Co-IP, sumoylation assay, ubiquitination assay, SAP domain deletion/mutation, siRNA knockdown, cell viability assay Signal transduction and targeted therapy Medium 32317623
2023 NUSAP1 interacts with ILF2 and DHX9 (RNA-binding proteins). The microtubule-binding and charged helical domains of NUSAP1 are required for these protein-protein interactions. Depletion of NUSAP1 abolished ILF2-dependent suppression of R-loop accumulation and DNA damage in response to camptothecin, placing NUSAP1 upstream of ILF2 in DNA damage/R-loop regulation. Affinity purification-mass spectrometry, Co-IP, confocal colocalization, domain deletion analysis, siRNA knockdown with R-loop and DNA damage assays International journal of molecular sciences High 37047232
2014 NUSAP1 depletion suppresses double-strand DNA break repair via homologous recombination and single-strand annealing pathways, and causes centrosome duplication defects. These phenotypes are mediated through BRCA1 protein levels: NUSAP1 depletion decreases BRCA1 protein and reduces BRCA1 recruitment to ionizing radiation-induced foci; BRCA1 overexpression from a plasmid rescues the defects caused by NUSAP1 depletion. siRNA knockdown, DNA repair assays (HR, SSA), centrosome counting, immunofluorescence for BRCA1 foci, plasmid rescue experiment Cancer biology & therapy Medium 24521615
2023 NUSAP1 binds to c-Myc and HIF-1α, forming a transcription regulatory complex that localizes to the LDHA promoter region and enhances LDHA expression. Lactate stabilizes NUSAP1 protein by inducing lysine lactylation (Kla) modification, inhibiting NUSAP1 protein degradation, creating a feedforward loop. ChIP-seq, Co-IP, RNA-seq, xenograft mouse model, single-cell RNA-seq, CHIP-seq verification of complex at LDHA promoter Cancer letters Medium 37354982
2021 NUSAP1 functions as a positive regulator of YAP1 protein stability in gastric cancer cells. NUSAP1 was identified as a novel binding partner of YAP1 by Co-IP, and NUSAP1 depletion reduces YAP1 levels and downstream Hippo target gene transcription (CTGF, CYR61). Cancer-promoting effects of NUSAP1 on cell growth, migration and invasion are largely mediated through YAP1. Co-IP, siRNA knockdown, xenograft model, epistasis rescue (YAP1-dependent phenotype) Frontiers in oncology Medium 33489890
2021 NUSAP1 interacts with CDC20 and CCNA2 (Cyclin A2) by co-immunoprecipitation in osteosarcoma cells. NUSAP1 accelerates cell proliferation and cell cycle progression, and silencing either CDC20 or CCNA2 inhibits NUSAP1-induced proliferation and cell cycle progression. Co-immunoprecipitation, siRNA knockdown, CCK-8 and EdU proliferation assays, flow cytometry, xenograft model OncoTargets and therapy Medium 34079289
2022 NUSAP1 depletion in glioblastoma cells leads to downregulation of TOP2A, and silencing either NUSAP1 or TOP2A inhibits GBM cell proliferation and invasion and induces apoptosis. NUSAP1 expression is strongly correlated with TOP2A in glioma datasets, and stable NUSAP1 knockdown reduces tumor growth in an orthotopic xenograft model. RNA sequencing of NUSAP1-stable knockdown cells, siRNA knockdown of NUSAP1 and TOP2A, proliferation/invasion assays, orthotopic xenograft Cell cycle (Georgetown, Tex.) Medium 35532155
2015 NUSAP1 expression is regulated by the RB1/E2F1 axis: RB1 knockdown increases NUSAP1 expression, and E2F1 knockdown decreases it. NUSAP1 knockdown in RB1-null or RB1-depleted prostate cancer cells decreases cell proliferation and invasion. Lentiviral RB1 knockdown, transient E2F1 siRNA, RT-qPCR, Western blot, proliferation and invasion assays The Prostate Medium 25585568
2022 HOXB2 transcriptionally activates NUSAP1; dual-luciferase reporter and chromatin immunoprecipitation assays confirmed that HOXB2 binds the NUSAP1 promoter. HOXB2 overexpression abrogated the inhibitory effects of NUSAP1 silencing on nephroblastoma cell proliferation and metastasis via the PI3K/Akt signaling pathway. Dual-luciferase reporter assay, ChIP assay, JASPAR bioinformatics, Western blot for PI3K/Akt pathway proteins, siRNA and overexpression transfection Molecular medicine reports Medium 35485274
2023 Transcription factor E2F8 directly binds the NUSAP1 promoter (confirmed by ChIP and dual-luciferase reporter) and regulates NUSAP1 transcription. The E2F8/NUSAP1 axis promotes cisplatin resistance in hepatocellular carcinoma by reducing DNA damage (γ-H2AX), with NUSAP1 knockdown sensitizing cells to cisplatin. ChIP assay, dual-luciferase reporter, siRNA knockdown, CCK-8, flow cytometry, comet assay, Western blot International journal of toxicology Medium 37331996
2025 PRMT1 catalyzes asymmetric dimethylation of NUSAP1 at R418 and R422 (ADMA). The R422 methylation site is critical for NUSAP1 function: methylated NUSAP1 interacts with the PEST domain of Notch2, inhibiting Notch2 ubiquitination and stabilizing Notch2 protein to activate Notch2 signaling. Inhibition of PRMT1 or R422 mutation abolishes NUSAP1-mediated Notch2 stabilization and 5-FU resistance in gastric cancer. Proteomic analysis, site-directed mutagenesis, Co-IP, ubiquitination assay, Western blot, functional resistance assays Cell death & disease Medium 40393971
2021 O-GlcNAcylation (mediated by OGT) enhances NUSAP1 protein stability in bladder cancer cells. Pharmacological upregulation of O-GlcNAc increases NUSAP1 protein levels and stability, and OGT knockdown reduces tumor growth, while NUSAP1 overexpression impairs this effect, placing NUSAP1 downstream of OGT-mediated O-GlcNAcylation. Western blot, pharmacological O-GlcNAc modulation (GlcNAc, PuGNAc), OGT siRNA, CCK-8, flow cytometry, xenograft OncoTargets and therapy Low 33488099
2022 ANKRD22 regulates NUSAP1 expression, and NUSAP1 overexpression reverses the inhibitory effects of ANKRD22 knockdown on breast cancer cell proliferation, invasion, and EMT. NUSAP1 mediates ANKRD22 activation of the Wnt/β-catenin signaling pathway. siRNA knockdown, overexpression rescue assay, Western blot for β-catenin pathway, BrdU, colony formation, Transwell assays Bosnian journal of basic medical sciences Low 32651974
2022 NUSAP1 promotes early recurrence of hepatocellular carcinoma by stimulating STAT3 nuclear translocation and activation through RACK1. RNA sequencing showed enrichment of cancer stemness and STAT3 pathway gene sets upon NUSAP1 overexpression. RNA sequencing, gain/loss-of-function experiments, in vivo recurrence mouse model, pathway analysis Cancer science Low 36106345
2025 NUSAP1 functions as a tumor suppressor in triple-negative breast cancer by recruiting the transcriptional repressor DAXX via its microtubule-associated domain (MAD) to bridge HIFα. This complex recruits SETDB1 methyltransferase and deposits H3K9me3 repressive marks on hypoxia response elements (HREs), attenuating HIF transcriptional activity and expression of HIF target genes. NUSAP1 depletion/overexpression, in vitro and in vivo invasion/proliferation assays, Co-IP (NUSAP1-DAXX-HIFα), ChIP for H3K9me3 at HREs, engineered MAD domain rescue Advanced science (Weinheim, Baden-Wurttemberg, Germany) Medium 41178464
2024 NUSAP1 interacts with ANXA2 (Annexin A2) and protects it from ubiquitin-dependent protein degradation, thereby increasing ANXA2 stability. NUSAP1 silencing increases GC radiosensitivity by impairing DNA damage repair after irradiation. Immunoprecipitation, mass spectrometry, ubiquitination assay, colony formation, flow cytometry, comet assay, xenograft Journal of cancer research and clinical oncology Medium 39212774
2025 NUSAP1 interacts with SHCBP1 and inhibits JAK2/STAT3 phosphorylation by blocking SHCBP1 in hepatocellular carcinoma cells, promoting dendritic cell generation from co-cultured PBMCs. Co-immunoprecipitation, Western blot for phospho-JAK2/STAT3, PBMC co-culture with HCC cells, flow cytometry for CD1a/CD86 Journal of immunotherapy Low 38980111
2025 NUSAP1 directly interacts with KIF2C (a kinesin-13 microtubule depolymerase) and Aurora kinase A (AURKA). AURKA-mediated phosphorylation of NUSAP1 modulates NUSAP1's binding affinity to KIF2C, balancing spindle microtubule stability and depolymerization. Truncating NUSAP1 mutations (Y403* and Q405*) impair NUSAP1-KIF2C interaction. NUSAP1 rescues cell cycle arrest and apoptosis caused by KIF2C overexpression in NUSAP1-KO cells, but mutant truncated NUSAP1 fails this rescue. Co-IP (NUSAP1-KIF2C, NUSAP1-AURKA), phosphorylation assay, NUSAP1-KO HEK293T rescue experiment, mouse microcephaly model, live cell imaging bioRxivpreprint Medium bio_10.1101_2025.05.16.654427
2023 A recurrent de novo heterozygous nonsense variant (p.Tyr403Ter) in NUSAP1 causes microcephaly, severe developmental delay, and epilepsy. The mutant transcript escapes nonsense-mediated decay, suggesting dominant-negative or gain-of-function mechanism rather than haploinsufficiency. Exome sequencing, NMD escape assay (transcript analysis), single-cell RNA-seq of post-mortem brain Clinical genetics Medium 37005340
2019 In zebrafish, nusap1 morpholino knockdown impairs neural crest cell migration, alters expression of neural crest markers crestin and sox9b, and causes apoptosis in retina and hindbrain. These phenotypes are rescued by co-injection of nusap1 mRNA. This establishes an in vivo role for Nusap1 in cell migration and neural crest morphogenesis. Antisense morpholino knockdown in zebrafish, in situ hybridization for neural crest markers, mRNA rescue injection Protein & cell Medium 21203972
2023 In mouse oocytes, NUSAP1 localizes to distinct granular aggregates near spindle poles during Pro-MI, metaphase I, and anaphase I. NUSAP1 depletion causes chromosome misalignment, increased aneuploidy, abnormal spindle assembly with decreased spindle pole width, and attenuation of cortical F-actin. Mass spectrometry of NUSAP1 interactomes showed significant enrichment for RNA-binding partners, suggesting NUSAP1 influences P-body dynamics and mRNA homeostasis. Protein expression profiling (proteomics), siRNA depletion, immunofluorescence for localization, aneuploidy measurement, RNA-seq, mass spectrometry interactome Journal of cellular biochemistry Medium 37992207
2025 NUSAP1 interacts with NUSAP1-IP3R (inositol 1,4,5-trisphosphate receptor); NUSAP1 governs IP3R phosphorylation status to regulate cytosolic Ca2+ homeostasis and ER stress. NUSAP1 knockdown increases cytosolic Ca2+, p-IP3R, and ER stress, promoting mitophagy and apoptosis in endometrial carcinoma cells. IP3R silencing reduces Ca2+ and ER stress without affecting NUSAP1 expression, but NUSAP1 overexpression combined with IP3R knockdown amplified these effects synergistically. Co-IP (NUSAP1-IP3R interaction), Fluo-4 AM Ca2+ measurement, ER fluorescence probe imaging, flow cytometry, Western blot, siRNA knockdown and overexpression ACS omega Low 41585655
2025 HBV core protein (HBC) enhances WDR46 protein stability by blocking TRIM25-mediated ubiquitination of WDR46. WDR46 then facilitates c-Myc recruitment to the NUSAP1 promoter by enhancing the WDR46-c-Myc interaction, thereby transcriptionally upregulating NUSAP1 to promote HCC cell growth and migration. Co-immunoprecipitation, ChIP, Western blot, ubiquitination assay, functional growth and migration assays, xenograft model Hepatology communications Medium 40366140
2024 NUSAP1 promotes lung adenocarcinoma cell proliferation, and its upregulation is mediated by estrogen via ERβ activation. Pharmacological targeting of the ERβ/NUSAP1 axis with fulvestrant (ERβ antagonist) or entinostat (identified as a novel NUSAP1 inhibitor) suppresses LUAD growth in vitro and in vivo. Bioinformatics, in vitro proliferation assays with estrogen treatment, ERβ activation/inhibition, entinostat treatment, in vivo xenograft International journal of biological sciences Low 39430250

Source papers

Stage 0 corpus · 65 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2023 NUSAP1-LDHA-Glycolysis-Lactate feedforward loop promotes Warburg effect and metastasis in pancreatic ductal adenocarcinoma. Cancer letters 146 37354982
2017 NUSAP1 promotes invasion and metastasis of prostate cancer. Oncotarget 65 28404898
2020 NUSAP1 potentiates chemoresistance in glioblastoma through its SAP domain to stabilize ATR. Signal transduction and targeted therapy 61 32317623
2018 Nucleolar and Spindle Associated Protein 1 (NUSAP1) Inhibits Cell Proliferation and Enhances Susceptibility to Epirubicin In Invasive Breast Cancer Cells by Regulating Cyclin D Kinase (CDK1) and DLGAP5 Expression. Medical science monitor : international medical journal of experimental and clinical research 53 30476929
2019 NUSAP1 knockdown inhibits cell growth and metastasis of non-small-cell lung cancer through regulating BTG2/PI3K/Akt signaling. Journal of cellular physiology 44 31603257
2015 NUSAP1 expression is upregulated by loss of RB1 in prostate cancer cells. The Prostate 39 25585568
2015 Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma. PloS one 38 26554377
2021 ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression. Bosnian journal of basic medical sciences 37 32651974
2021 NUSAP1 Promotes Gastric Cancer Tumorigenesis and Progression by Stabilizing the YAP1 Protein. Frontiers in oncology 32 33489890
2014 NUSAP1 influences the DNA damage response by controlling BRCA1 protein levels. Cancer biology & therapy 30 24521615
2010 Antibodies specifically target AML antigen NuSAP1 after allogeneic bone marrow transplantation. Blood 30 20053754
2017 Nucleolar and spindle-associated protein 1 (NUSAP1) interacts with a SUMO E3 ligase complex during chromosome segregation. The Journal of biological chemistry 28 28900032
2021 NUSAP1 promotes the metastasis of breast cancer cells via the AMPK/PPARγ signaling pathway. Annals of translational medicine 25 34988198
2020 Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway. OncoTargets and therapy 25 32099387
2019 Hepatitis B virus X protein enhances hepatocarcinogenesis by depressing the targeting of NUSAP1 mRNA by miR-18b. Cancer biology & medicine 24 31516748
2019 Downregulation of NUSAP1 suppresses cell proliferation, migration, and invasion via inhibiting mTORC1 signalling pathway in gastric cancer. Cell biochemistry and function 24 31710389
2023 NUSAP1 Binds ILF2 to Modulate R-Loop Accumulation and DNA Damage in Prostate Cancer. International journal of molecular sciences 21 37047232
2022 RNA-Binding Protein COL14A1, TNS1, NUSAP1 and YWHAE Are Valid Biomarkers to Predict Peritoneal Metastasis in Gastric Cancer. Frontiers in oncology 20 35515139
2010 Nusap1 is essential for neural crest cell migration in zebrafish. Protein & cell 20 21203972
2022 NUSAP1, a novel stemness-related protein, promotes early recurrence of hepatocellular carcinoma. Cancer science 19 36106345
2021 O-GlcNAcylation Enhances NUSAP1 Stability and Promotes Bladder Cancer Aggressiveness. OncoTargets and therapy 18 33488099
2020 MicroRNA-769-5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer. Journal of clinical laboratory analysis 18 31901150
2022 NUSAP1 Could be a Potential Target for Preventing NAFLD Progression to Liver Cancer. Frontiers in pharmacology 17 35431924
2021 NUSAP1 Accelerates Osteosarcoma Cell Proliferation and Cell Cycle Progression via Upregulating CDC20 and Cyclin A2. OncoTargets and therapy 17 34079289
2022 Transcription factor HOXB2 upregulates NUSAP1 to promote the proliferation, invasion and migration of nephroblastoma cells via the PI3K/Akt signaling pathway. Molecular medicine reports 16 35485274
2022 Knockdown of NUSAP1 inhibits cell proliferation and invasion through downregulation of TOP2A in human glioblastoma. Cell cycle (Georgetown, Tex.) 15 35532155
2023 LINC01393, a Novel Long Non-Coding RNA, Promotes the Cell Proliferation, Migration and Invasion through MiR-128-3p/NUSAP1 Axis in Glioblastoma. International journal of molecular sciences 13 36982952
2018 SWATH proteomic profiling of prostate cancer cells identifies NUSAP1 as a potential molecular target for Galiellalactone. Journal of proteomics 13 30626528
2015 High EGFR_1 Inside-Out Activated Inflammation-Induced Motility through SLC2A1-CCNB2-HMMR-KIF11-NUSAP1-PRC1-UBE2C. Journal of Cancer 12 26000042
2021 Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression. Open medicine (Warsaw, Poland) 11 34322597
2024 NUSAP1 promotes pancreatic ductal adenocarcinoma progression by drives the epithelial-mesenchymal transition and reduces AMPK phosphorylation. BMC cancer 10 38229038
2024 NUSAP1 promotes gastric cancer radioresistance by inhibiting ubiquitination of ANXA2 and is suppressed by miR-129-5p. Journal of cancer research and clinical oncology 10 39212774
2023 Transcription Factor E2F8 Promotes Cisplatin Resistance in Hepatocellular Carcinoma by Regulating DNA Damage via NUSAP1. International journal of toxicology 10 37331996
2021 Analyzing Roles of NUSAP1 From Clinical, Molecular Mechanism and Immune Perspectives in Hepatocellular Carcinoma. Frontiers in genetics 10 34354737
2019 Nucleolar spindle associated protein 1 (NUSAP1) facilitates proliferation of hepatocellular carcinoma cells. Translational cancer research 10 35116961
2022 microRNA-569 inhibits tumor metastasis in pancreatic cancer by directly targeting NUSAP1. Aging 9 35483343
2023 A recurrent de novo variant in NUSAP1 escapes nonsense-mediated decay and leads to microcephaly, epilepsy, and developmental delay. Clinical genetics 8 37005340
2013 [Expression of Nusap1 in the surgical margins of hepatocellular carcinoma and its association with early recurrence]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University 8 23803217
2013 [Expression and clinical significance of Nusap1 in hepatical carcinoma]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 8 24071699
2024 NUSAP1 Promotes Immunity and Apoptosis by the SHCBP1/JAK2/STAT3 Phosphorylation Pathway to Induce Dendritic Cell Generation in Hepatocellular Carcinoma. Journal of immunotherapy (Hagerstown, Md. : 1997) 7 38980111
2023 NUSAP1 regulates mouse oocyte meiotic maturation. Journal of cellular biochemistry 7 37992207
2024 Unveiling NUSAP1 as a common gene signature linking chronic HBV infection and HBV-related HCC. Discover oncology 6 38441732
2024 NUSAP1 is Upregulated by Estrogen to Promote Lung Adenocarcinoma Growth and Serves as a Therapeutic Target. International journal of biological sciences 6 39430250
2023 NUSAP1 regulates basal cell carcinoma migration, invasion and DNA damage through activation of the Hedgehog signaling pathway. Physiology international 6 37200136
2023 Distinct Mitotic Functions of Nucleolar and Spindle-Associated Protein 1 (NuSAP1) Are Controlled by Two Consensus SUMOylation Sites. Cells 5 37947624
2020 [NUSAP1 promotes lung cancer progression by activating AKT/mTOR signaling pathway]. Zhonghua zhong liu za zhi [Chinese journal of oncology] 5 32842441
2025 Silencing long non-coding RNA linc00689 suppresses the growth and invasion of osteosarcoma cells by targeting miR-129-5p/NUSAP1. International journal of experimental pathology 4 39891384
2025 HBV core protein enhances WDR46 stabilization to upregulate NUSAP1 and promote HCC progression. Hepatology communications 4 40366140
2024 Immunotherapeutic value of NUSAP1 associated with bladder cancer through a comprehensive analysis of 33 human cancer cases. American journal of cancer research 4 38590423
2022 NUSAP1 and PCLAF (KIA0101) Downregulation by Neoadjuvant Therapy is Associated with Better Therapeutic Outcomes and Survival in Breast Cancer. Journal of oncology 4 36478748
2021 [miR-758-3p inhibits the proliferation and invasion of non-small cell lung cancer cells by targeting NUSAP1]. Zhonghua zhong liu za zhi [Chinese journal of oncology] 4 33472323
2021 Corrigendum: NUSAP1 Promotes Gastric Cancer Tumorigenesis and Progression by Stabilizing the YAP1 Protein. Frontiers in oncology 4 33834001
2025 The role and therapeutic value of NUSAP1 in human cancers. Journal of translational medicine 3 40605080
2025 TRIP13-induced NUSAP1 upregulation promotes CcRCC progression through EMT and PI3K/AKT/mTOR pathway. Journal of translational medicine 3 40790482
2025 In Silico Analysis Revealed a Role for NUSAP1 in Lung Adenocarcinoma through E2F1/hsa-let-7b-5p/lncRNA-TMPO-AS1. Asian Pacific journal of cancer prevention : APJCP 2 40439393
2025 NUSAP1 Recruits DAXX to Suppress HIF-Driven Triple-Negative Breast Cancer Progression. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2 41178464
2024 MiR-490-3p promotes cell apoptosis and cell-cycle arrest in osteosarcoma via the modulation of CDCA8/ATF3 by targeting NUSAP1. Translational pediatrics 2 39823004
2026 NUSAP1 Drives Endometrial Carcinoma via IP3R Phosphorylation, Calcium Dyshomeostasis, and ER-Stress-Mediated Apoptosis. ACS omega 1 41585655
2025 PRMT1-catalyzed NUSAP1 methylation enhances Notch2 signaling and 5-FU resistance in gastric cancer. Cell death & disease 1 40393971
2025 Research progress on NUSAP1 and its role in digestive system neoplasms. Frontiers in oncology 1 40535133
2023 Retracted: Chen, Y, Zhang, W, Kadier, A, Zhang, H, Yao, X. MicroRNA-769-5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer. J Clin Lab Anal. 2020; 34:e23193. Journal of clinical laboratory analysis 1 37183304
2026 CCNB2, NUSAP1, GTSE1, and TK1: potential dual biomarkers for pneumonia and lung adenocarcinoma. Cytotechnology 0 41908258
2025 NuSAP1 promotes bipolar spindle assembly in Trypanosoma brucei by bundling spindle microtubules. bioRxiv : the preprint server for biology 0 40909470
2025 Research Article: In Silico Identification of Potential Inhibitors Targeting NUSAP1, A Hub Gene in Hepatocellular Carcinoma. Critical reviews in oncogenesis 0 41275481
2024 Multi-omics analysis detail a submicroscopic inv(15)(q14q15) generating fusion transcripts and MEIS2 and NUSAP1 haploinsufficiency. Scientific reports 0 39639090

Missed literature

Know a paper Affinage missed for NUSAP1? Flag it for the maintainers and the community.

No submissions yet.