Affinage

NID1

Nidogen-1 · UniProt P14543

Length
1247 aa
Mass
136.4 kDa
Annotated
2026-04-29
94 papers in source corpus 37 papers cited in narrative 37 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NID1 (nidogen-1/entactin) is a secreted basement membrane glycoprotein that bridges laminin to type IV collagen and fibronectin, thereby organizing and stabilizing basement membrane architecture (PMID:2119632, PMID:8433553). Its multidomain structure harbors two distinct cell-adhesion sites: an RGD sequence in the EGF-like E domain that engages αvβ3 integrin and the leukocyte response integrin to mediate cell attachment and neutrophil chemotaxis, and a cysteine-rich repeat in the G2 domain that binds α3β1 integrin to promote adhesion and enhance phagocytosis (PMID:7797588, PMID:8940031, PMID:1469085). In vivo, NID1 loss selectively disrupts brain capillary and lens capsule basement membranes causing neurological deficits and altered glomerular permselectivity, and mutations in NID1 and its partner LAMC1 cause autosomal dominant Dandy-Walker malformation (PMID:12480912, PMID:14566019, PMID:23674478). In cancer contexts, NID1 is transcriptionally activated by SNAIL, HIF-1α, and the RUNX2/BRD4 axis, and functions as a secreted paracrine signal that drives epithelial-mesenchymal transition and metastasis through ERK/MAPK and PI3K/AKT pathways (PMID:38001576, PMID:32157097, PMID:35689951, PMID:28416770).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1981 High

    Identification of entactin as a distinct sulfated basement membrane glycoprotein resolved the question of whether basement membranes contained components beyond laminin and collagen IV.

    Evidence Biochemical isolation and immunoelectron microscopy of rodent kidney

    PMID:6262321

    Open questions at the time
    • Protein sequence unknown
    • Binding partners not identified
    • Function in BM assembly not tested
  2. 1988 High

    cDNA sequencing revealed the multidomain architecture (G1-rod-G2 with EGF repeats, thyroglobulin repeat, and an RGD sequence), establishing that entactin possesses intrinsic cell-adhesion capacity through a defined integrin-recognition motif.

    Evidence cDNA cloning, sequence analysis, and RGD peptide cell attachment assay in mammary tumor cells

    PMID:3264556

    Open questions at the time
    • Integrin receptor identity unknown
    • Domains mediating BM protein interactions not mapped
  3. 1990 High

    Demonstration that entactin forms a stable intracellular complex with laminin and that its transfection into cells lacking entactin stimulates incorporation of laminin and collagen IV into matrix established entactin as a critical bridging molecule in basement membrane assembly.

    Evidence Transfection of entactin cDNA into JAR cells lacking entactin, with extracellular matrix fractionation and immunofluorescence

    PMID:2119632 PMID:2191952

    Open questions at the time
    • Binding domains on laminin and collagen IV not mapped
    • In vivo requirement not tested
  4. 1991 High

    Discovery of direct entactin binding to fibrinogen and fibronectin expanded its interaction repertoire beyond classical BM components, suggesting roles in wound healing and provisional matrix.

    Evidence Solid-phase binding assays, affinity chromatography, and transglutaminase cross-linking

    PMID:1680863 PMID:1872841

    Open questions at the time
    • In vivo relevance of fibrinogen/fibronectin interactions not shown
    • Fibronectin-binding domain not mapped
  5. 1992 High

    Identification of α3β1 as an RGD-independent entactin receptor and of the leukocyte response integrin as the RGD-dependent receptor for neutrophil chemotaxis established that entactin engages at least two distinct integrin pathways with different biological outcomes.

    Evidence Affinity chromatography on entactin-Sepharose, anti-integrin antibody blocking, RGD→RGE site-directed mutagenesis with neutrophil chemotaxis and adhesion assays

    PMID:1469085 PMID:1527019

    Open questions at the time
    • Signaling cascades downstream of each integrin not defined
    • In vivo relevance of neutrophil chemotaxis to entactin not demonstrated
  6. 1993 High

    Kinetic characterization of MMP-7 (matrilysin) as the most efficient protease for entactin degradation, with cleavage at Leu/Ile-preceding sites, provided a mechanism for regulated BM disassembly.

    Evidence In vitro protease digestion kinetics and Edman degradation for cleavage-site identification

    PMID:8380588

    Open questions at the time
    • In vivo regulation of entactin by MMP-7 not shown at this time
    • Functional consequences of individual fragments not fully characterized
  7. 1995 High

    Domain dissection mapped two distinct cell-adhesion sites — the RGD in the E domain engaging αvβ3 and a cysteine-rich EGF repeat in G2 engaging β1 integrin — revealing that entactin is a bifunctional adhesion molecule with domain-specific integrin selectivity.

    Evidence GST-domain fusion protein cell attachment assays, baculovirus-expressed RGD deletion mutant, and anti-integrin antibody blocking

    PMID:7797588

    Open questions at the time
    • Precise β1 integrin heterodimer at G2 site debated (α3β1 vs others)
    • Structural basis of G2–integrin interaction unknown
  8. 1996 High

    Functional separation showed the E domain/RGD drives neutrophil chemotaxis via LRI while the G2 domain/α3β1 enhances phagocytosis, and in vivo MMP-3-mediated entactin cleavage in mammary gland was directly linked to epithelial apoptosis, providing the first in vivo consequence of entactin proteolysis.

    Evidence Domain-specific neutrophil activation assays; transgenic mouse cross of stromelysin-1 and TIMP-1 with apoptosis readout

    PMID:8940031 PMID:8978831

    Open questions at the time
    • Whether entactin fragments act as direct apoptotic signals or loss of BM support is uncertain
    • Receptor for apoptotic signaling not identified
  9. 2002 High

    Nid1-knockout mice revealed selective vulnerability of brain capillary and lens capsule BMs, with neurological deficits and altered glomerular permselectivity, establishing that NID1 is non-redundant in specific tissues despite compensation elsewhere.

    Evidence Gene-targeted knockout mouse with behavioral, immunohistochemical, and electron microscopy phenotyping; glomerular filtration analysis

    PMID:12480912 PMID:14566019

    Open questions at the time
    • Compensatory role of nidogen-2 not fully delineated
    • Molecular basis of tissue-selective BM sensitivity unknown
  10. 2013 Medium

    Whole-exome sequencing linked NID1 mutations to autosomal dominant Dandy-Walker malformation, with structural modeling showing disruption of the NID1-LAMC1 interaction, connecting NID1 to a human developmental brain disorder.

    Evidence Whole-exome sequencing of families with Dandy-Walker malformation, structural modeling of NID1-LAMC1 complex

    PMID:23674478

    Open questions at the time
    • Small number of families; independent replication in larger cohorts not reported
    • No functional rescue experiment confirming causality of specific mutations
  11. 2017 Medium

    NID1 was shown to activate ERK/MAPK signaling to promote EMT in cancer cells, opening a new functional dimension as a secreted pro-metastatic factor beyond its classical BM structural role.

    Evidence Ectopic overexpression and siRNA knockdown in ovarian cancer cells with EMT marker, migration/invasion, and pathway analysis

    PMID:28416770

    Open questions at the time
    • Receptor mediating ERK activation not identified
    • In vivo metastasis not demonstrated in this study
  12. 2019 High

    The p53/miR-192/215 axis was identified as a negative regulator of NID1 expression, and NID1-containing conditioned medium was shown to induce EMT in epithelial CRC cells in a paracrine manner, establishing NID1 as a secreted intercellular EMT signal regulated by tumor suppressors.

    Evidence Conditioned medium transfer, miRNA target validation, p53 activation experiments in CRC cell lines

    PMID:30831320

    Open questions at the time
    • Receptor on recipient cells not identified in this study
    • Contribution of other secreted factors in conditioned medium not fully excluded
  13. 2020 High

    ChIP demonstrated direct RUNX2 binding at the NID1 promoter, placing NID1 downstream of the BRD4/RUNX2 transcriptional axis and explaining how BET inhibitors suppress metastasis in gastric cancer.

    Evidence ChIP, luciferase reporter, ATAC-seq/RNA-seq, and in vivo tumor models in gastric cancer

    PMID:32157097

    Open questions at the time
    • Whether RUNX2 regulation of NID1 operates in non-cancer contexts unknown
    • Other transcriptional regulators of NID1 not surveyed systematically
  14. 2022 Medium

    HIF-1α was shown to directly bind the NID1 promoter and drive transcription under hypoxia, linking NID1 upregulation to the hypoxic tumor microenvironment and PI3K/AKT-mediated metastasis.

    Evidence ChIP and dual-luciferase reporter in salivary gland adenoid cystic carcinoma cells; in vivo lung metastasis model

    PMID:35689951

    Open questions at the time
    • Whether HIF-1α regulation is tissue-general or cancer-specific not determined
    • Direct NID1 receptor activating PI3K/AKT not identified
  15. 2023 High

    SNAIL was identified as a direct transcriptional activator of NID1 through E-box occupancy, and ITGAV (αv integrin) was shown to be the primary NID1 receptor mediating paracrine lung metastasis in CRC, closing a key gap in receptor identity.

    Evidence ChIP for SNAIL at NID1 E-box, conditioned medium xenograft lung metastasis assay, ITGAV knockdown in CRC cells

    PMID:38001576

    Open questions at the time
    • Whether ITGAV partners with β3 or another β subunit in this context not resolved
    • Downstream signaling from ITGAV/NID1 not fully mapped
  16. 2025 Medium

    NID1 was placed in the JAK2/STAT3/IL-6 autocrine signaling axis in hepatic stellate cells, establishing a role for NID1 in NASH-related liver fibrosis beyond its classical BM and cancer contexts.

    Evidence CDAHFD mouse model of NASH, AAV8-mediated Nid1 knockdown, recombinant Nid1 rescue, JAK2/STAT3 pathway analysis

    PMID:41349744

    Open questions at the time
    • Mechanism by which NID1 activates JAK2 not defined
    • Whether NID1 acts through a cell-surface receptor or intracellularly in stellate cells unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: how NID1 selectively activates distinct signaling cascades (ERK, PI3K/AKT, JAK2/STAT3) in different cellular contexts; the structural basis of integrin heterodimer selectivity at the G2 domain; and whether nidogen-2 compensates for NID1 loss in tissues that appear unaffected in knockouts.
  • No structural model of NID1–integrin complexes exists
  • Systematic comparison of NID1 vs NID2 function in vivo is lacking
  • Receptor identity for JAK2/STAT3 activation is unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098631 cell adhesion mediator activity 5 GO:0005198 structural molecule activity 3 GO:0060089 molecular transducer activity 3
Localization
GO:0005576 extracellular region 4 GO:0031012 extracellular matrix 4 GO:0005794 Golgi apparatus 1
Pathway
R-HSA-1474244 Extracellular matrix organization 4 R-HSA-1500931 Cell-Cell communication 4 R-HSA-162582 Signal Transduction 3
Partners
COL4A1FN1HSPG2ITGA3ITGAVITGB1ITGB3LAMC1
Complex memberships
Laminin-nidogen complex

Evidence

Reading pass · 37 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1981 Entactin (NID1) is a sulfated glycoprotein component of basement membranes, distinct from laminin (GP-2), with a molecular weight of ~158 kDa, localized at the surface of epithelial cells adjacent to basement membranes in rodent kidney and other tissues. Biochemical isolation, immunoelectron microscopy, antibody-based characterization The Journal of biological chemistry High 6262321
1988 Mouse entactin is organized into two globular domains (70 kDa N-terminal and 36 kDa C-terminal) separated by a cysteine-rich 28 kDa stalk; the C-terminal domain shows homology to EGF precursor and LDL receptor; the molecule contains six EGF-type cysteine-rich repeats, one thyroglobulin repeat, and an RGD cell-recognition sequence in one EGF-type repeat; synthetic RGD-containing peptide promotes mammary tumor cell attachment. cDNA sequencing, sequence analysis, synthetic peptide cell attachment assay The Journal of cell biology High 3264556
1988 Entactin tyrosine sulfation occurs in the medial Golgi cisternae and is not the last modification before secretion; N-linked glycosylation and terminal glycosylation in the trans-Golgi occur after sulfation. Metabolic labeling with [35S]methionine and H2[35S]O4, tunicamycin and monensin inhibitor treatments, pulse-chase analysis FEBS letters Medium 3042455
1990 Entactin promotes cell attachment through the integrin RGD recognition sequence; additionally, entactin directly binds calcium ions through sites in the N-terminal region, demonstrated with recombinant peptides containing the first 330 amino acids. Cell attachment assay with synthetic peptides and recombinant entactin, calcium binding assay with recombinant N-terminal fragment The Journal of biological chemistry High 2191952
1990 Entactin forms a highly stable noncovalent complex with laminin intracellularly; transfection of entactin into JAR choriocarcinoma cells (which lack entactin but make laminin and collagen IV) stimulates incorporation of all three into extracellular matrix, indicating entactin bridges laminin and type IV collagen in basement membrane assembly. Cell transfection, immunofluorescence, extracellular matrix fractionation American journal of respiratory cell and molecular biology High 2119632
1991 Entactin specifically binds fibrinogen through interactions with the Aα and Bβ chains of fibrinogen in a divalent cation-independent manner; entactin can be cross-linked to fibrinogen by transglutaminase. Solid phase binding assay with radiolabeled entactin, antibody inhibition, transglutaminase cross-linking The Journal of biological chemistry High 1680863
1991 Entactin forms a complex with fibronectin (independent of laminin) and co-localizes in the extracellular matrix of 4CQ embryonal carcinoma cells; direct binding between entactin and fibronectin was demonstrated by affinity chromatography and solid phase assay. Affinity column chromatography, solid phase binding assay, immunofluorescence Biochemical and biophysical research communications Medium 1872841
1992 The cell surface receptor for entactin on PC-3 prostate carcinoma cells is integrin α3β1; binding requires divalent cations (Ca2+, Mg2+, Mn2+) and is not inhibited by RGD-containing peptides; α3β1 purified from cells binds entactin-Sepharose, while α2β1 does not. Affinity chromatography on entactin-Sepharose, anti-integrin antibody identification, purified integrin binding assay, liposome reconstitution, antibody inhibition of cell attachment The Journal of biological chemistry High 1527019
1992 Entactin mediates neutrophil (PMN) adhesion and chemotaxis through its RGD domain and the leukocyte response integrin (LRI); a recombinant RGE-substituted entactin mutant (Asp674→Glu) lost both adhesive and chemotactic activities; anti-β1 and anti-β2 antibodies did not block the response whereas anti-LRI did. Adhesion assay, chemotaxis assay, RGD/RGE synthetic peptides, recombinant site-directed mutant entactin, monoclonal antibody blocking, leukocyte adhesion deficiency patient PMNs The Journal of clinical investigation High 1469085
1992 Entactin is required for long-term maintenance and adhesion of contractile skeletal myotubes; anti-entactin antibodies did not inhibit satellite cell attachment or fusion but caused myotube detachment after spontaneous contractions began, demonstrating a specific role for entactin in myotube maintenance. Anti-entactin antibody perturbation on Matrigel cultures of regenerated satellite cells, myotube adhesion assay Journal of cellular physiology Medium 1734030
1993 Entactin is specifically degraded by matrix metalloproteinases; matrilysin (MMP-7) is ~100-fold more effective than interstitial collagenase and ~600-fold more effective than 92 kDa gelatinase; matrilysin cleaves entactin at sites amino-terminal to leucine or isoleucine residues producing fragments of 29–115 kDa. In vitro protease digestion assay, kinetic analysis (Km, Vmax), Edman degradation for cleavage site identification The Journal of biological chemistry High 8380588
1993 Entactin binds laminin, collagen IV, fibrinogen, and fibronectin; the laminin-entactin complex is formed intracellularly in M1536-B3 cells and transported in membrane-enclosed vesicles to the extracellular compartment. Co-immunoprecipitation, cell fractionation, transfection experiments, indirect immunofluorescence Kidney international High 8433553
1993 Recombinant entactin promotes primary trophoblast cell adhesion and migration through the RGD recognition sequence; a mutated entactin with Glu replacing Asp at the RGD site provided no trophoblast adhesive activity. Blastocyst outgrowth assay, RGD peptide inhibition, recombinant RGD→RGE mutant entactin, anti-entactin antibody The Journal of cell biology High 8491783
1994 The binding of fibronectin to entactin is mediated through the 29 kDa amino-terminal fragment of fibronectin and the G2 domain of entactin (but not G1, E, or G3 domains); half-saturation for binding is ~5 nM. Solid phase binding assay with GST-domain fusion proteins of entactin and radiolabeled fibronectin fragment Biochemical and biophysical research communications Medium 8147897
1994 A novel epitope of entactin at the neuromuscular junction is dependent on glycosylation; N-glycanase treatment reduces entactin molecular mass and eliminates the synaptic-specific 9H6 antibody epitope, indicating that synaptic entactin bears a distinct N-glycosylation pattern not found at extrasynaptic sites. Monoclonal antibody generation, Western blot, N-glycanase treatment, immunostaining of NMJ The Journal of neuroscience Medium 7514212
1995 Entactin has two distinct cell attachment sites: (1) the RGD sequence in the EGF-like stalk (E domain) recognized by αvβ3 integrin, and (2) a second site in a 39-amino acid cysteine-rich EGF repeat of the G2 domain recognized by a β1 integrin family member; deletion/mutation of RGD reveals the second site. GST-domain fusion protein cell attachment assay, baculovirus-expressed full-length RGD deletion mutant, anti-integrin antibody inhibition The Journal of biological chemistry High 7797588
1996 Entactin E domain (containing RGD) ligates the leukocyte response integrin (LRI/β3-like) and signals for neutrophil chemotaxis, while the G2 domain ligates α3β1 and signals for enhancement of Fc receptor-mediated phagocytosis; matrilysin cleavage of entactin liberates fragments retaining both activities. GST-domain fusion protein neutrophil activation assays (chemotaxis, phagocytosis), matrilysin cleavage, anti-integrin antibody blocking The Journal of biological chemistry High 8940031
1996 Stromelysin-1 (MMP-3) cleaves entactin in mammary gland basement membrane in vivo; enhanced cleavage of entactin correlates directly with apoptosis of overlying mammary epithelial cells; TIMP-1 overexpression blocks both entactin cleavage and apoptosis. Transgenic mouse cross (stromelysin-1 × TIMP-1 overexpressing mice), apoptosis assay, in vivo entactin protein quantification The Journal of cell biology High 8978831
1998 Human mesangial cells adhere to native entactin via both αvβ3 (binding to the RGD sequence on the E domain) and a β1 integrin receptor (binding to a non-RGD site); cation dependence was demonstrated and tertiary molecular structure of entactin contributes to binding. Anti-integrin antibody inhibition of adhesion, wild-type and mutant recombinant entactin fragments, immunoprecipitation of integrin receptors Cell adhesion and communication Medium 9686320
2000 Nidogen-1/entactin-1 is exclusively produced and secreted by mesenchymal peritubular cells (not Sertoli cells) in the testis; monoclonal antibody perturbation against entactin-1 caused loss of peritubular cell adhesion (autocrine function) while Sertoli cells remained adherent. DD-RT-PCR, Western blotting of cell fractions and supernatants, monoclonal antibody perturbation of cell adhesion European journal of cell biology Medium 10727019
2001 Entactin inhibits amyloid β-protein (Aβ1-40) fibril formation in vitro in a dose-dependent manner at a molar ratio of 50:1 (Aβ:entactin); entactin induces a random coil structure in Aβ40 as shown by circular dichroism spectroscopy. Thioflavin T fluorometric assay, electron microscopy, circular dichroism spectroscopy Neuroscience letters Medium 11376898
2002 Targeted disruption of entactin-1/nidogen-1 in mice results in neurological deficits (seizure-like symptoms, loss of hind-leg muscle control) and selective structural alterations in basement membranes of brain capillaries and lens capsule, while other basement membranes appear morphologically normal. Gene targeting/knockout mouse, behavioral phenotyping, immunohistochemistry, electron microscopy Laboratory investigation High 12480912
2003 In entactin-1-null mice, the glomerular basement membrane is thickened; distribution of anionic charges is significantly altered; αv-integrin density on glomerular cells is increased; glomerular filtration permselectivity (albumin distribution) is altered; type IV collagen and laminin distributions remain unchanged. Immunocytochemistry in knockout mice, morphometry, glomerular filtration analysis with endogenous albumin The journal of histochemistry and cytochemistry Medium 14566019
2013 Mutations in NID1 and its binding partner LAMC1 cause autosomal dominant Dandy-Walker malformation; structural modeling of the NID1-LAMC1 complex shows each mutation disrupts the protein-protein interaction. Whole-exome sequencing, protein interaction network analysis, structural modeling of NID1-LAMC1 complex Human mutation Medium 23674478
2017 NID1 activates ERK/MAPK signaling to promote epithelial-mesenchymal transition (EMT) in ovarian cancer cells; ectopic NID1 expression induces EMT with enhanced motility, invasiveness, and cisplatin resistance, while NID1 knockdown reverses these effects. Ectopic overexpression and siRNA knockdown, EMT marker analysis, migration/invasion assays, ERK/MAPK pathway analysis Oncotarget Medium 28416770
2019 NID1 is secreted by mesenchymal-like colorectal cancer cells and induces EMT in neighboring epithelial-like CRC cells via paracrine signaling; p53 suppresses NID1 expression by inducing miR-192 and miR-215, which directly target the NID1 mRNA; NID1 is required and sufficient for inducing EMT in recipient cells. Conditioned medium transfer, cytokine array, miRNA target validation, p53 activation experiments, rescue assays Cellular and molecular gastroenterology and hepatology High 30831320
2019 Drosophila Nidogen/entactin (NDG) is not essential for basement membrane assembly but mediates BM stability and ECM-dependent neural plasticity; loss of Laminin strongly affects BM localization of NDG; Ndg-null mutants have ultrastructural BM defects compromising barrier function, impaired larval crawling, and defects in chordotonal organs and neuromuscular junction. Drosophila Ndg-null mutants, TEM ultrastructure, in vivo barrier function assay, behavioral assays, confocal immunostaining Development (Cambridge, England) High 30567930
2020 BRD4 inhibitor JQ1 reduces NID1 expression in gastric cancer cells via RUNX2; RUNX2 directly binds the NID1 promoter region (demonstrated by ChIP), and RUNX2/NID1 axis mediates JQ1-inhibited metastasis; NID1 knockdown inhibits migration and invasion by inducing MET. ATAC-seq, RNA-seq, ChIP, luciferase reporter assay, rescue experiments, in vivo tumor models Oncogenesis High 32157097
2021 Enteric neuron-derived Nidogen-1 (NID1) is secreted by enteric neurons and promotes colorectal cancer cell migration; the NID1-enriched secretome of Ndrg4-/- ENS cells boosts intestinal organoid growth; NID1 is enriched in human CRC secretomes. Ndrg4 knockout mouse models, indirect co-culture, quantitative proteomics, in vitro migration assay, immunostaining EMBO reports Medium 33890711
2022 NID1 overexpression promotes salivary gland adenoid cystic carcinoma (SACC) metastasis via PI3K/AKT pathway activation and subsequent EMT; HIF-1α directly binds the NID1 promoter and drives NID1 transcription under hypoxia, as confirmed by ChIP and dual-luciferase reporter assay. ChIP, dual-luciferase reporter assay, NID1 overexpression, PI3K/AKT pathway analysis, in vivo lung metastasis mouse model Oral oncology Medium 35689951
2022 Skeletal muscle fibro-adipogenic progenitors (FAPs) are the primary source of elevated NID1 during obesity; increased NID1 impairs muscle stem cell proliferation and primes FAPs toward fibrogenic differentiation, causing excessive ECM deposition. High-fat diet mouse model, cell fractionation, in vitro myoblast/stem cell assays with recombinant NID1, immunostaining of endomysium Matrix biology Medium 35963565
2023 NID1 is a direct transcriptional target of the EMT transcription factor SNAIL, which occupies an E-box upstream of the NID1 transcription start site; NID1-containing conditioned medium endows non-metastatic CRC cells with lung metastatic capacity after xenotransplantation; ITGAV (αv integrin) is the primary NID1 receptor mediating these effects in CRC. ChIP for SNAIL occupancy at NID1 E-box, conditioned medium transfer, xenograft lung metastasis assay, ITGAV knockdown Cancers High 38001576
2023 COL4A1 binds to NID1 (confirmed by co-immunoprecipitation) and promotes OSCC cell proliferation, migration, invasion, and EMT; NID1 overexpression reverses the inhibitory effects of COL4A1 knockdown, placing NID1 downstream of COL4A1. Co-immunoprecipitation, siRNA knockdown, NID1 overexpression rescue, cell proliferation and invasion assays, EMT marker analysis Experimental and therapeutic medicine Medium 37006878
2018 In C. elegans, NID-1/Nidogen is required for correct dendrite patterning of PVD somatosensory neurons; UNC-52/Perlecan localizes NID-1 via four conserved immunoglobulin domains; genetic epistasis places nid-1 in the same pathway as unc-52 and the netrin axon guidance signaling cassette for dendrite morphogenesis. C. elegans genetics, null mutant analysis, epistasis assay, confocal imaging of dendrite morphology Development (Cambridge, England) Medium 29678816
2025 In C. elegans, NID-1 expressed by body wall muscles or hypodermis promotes guidance of regenerating cholinergic motor axons alongside PVD dendrites; muscle-derived NID-1 specifically is required for synapse reformation and functional recovery; NID-1 acts in coordination with laminin and integrin for axon guidance. C. elegans nid-1 null mutant, tissue-specific rescue, axon regeneration assay, synapse reformation assay, genetic epistasis with laminin and integrin mutants bioRxivpreprint Medium 41890084
2025 Nid1 mediates NASH-related liver fibrosis by activating the JAK2/STAT3 pathway and IL-6 autocrine signaling in hepatic stellate cells; AAV8-mediated Nid1 knockdown attenuates fibrosis; recombinant Nid1 supplementation rescues the protective effect of CP treatment, confirming Nid1 as the mechanistic target. CDAHFD mouse model, AAV8-mediated knockdown, exogenous recombinant Nid1 rescue, co-culture systems, JAK2/STAT3 pathway analysis, proteomic/metabolomic analysis International journal of biological macromolecules Medium 41349744
2024 HSPG2 upregulates NID1 expression, leading to activation of the AKT pro-survival signaling pathway and promotion of bladder cancer cell proliferation and chemotherapy resistance. HSPG2 overexpression in bladder cancer cell lines, Western blotting and immunostaining for NID1/AKT activation, patient-derived tumor organoid mouse models Cancer cell international Low 39438949

Source papers

Stage 0 corpus · 94 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1981 Entactin, a novel basal lamina-associated sulfated glycoprotein. The Journal of biological chemistry 417 6262321
1996 Rescue of mammary epithelial cell apoptosis and entactin degradation by a tissue inhibitor of metalloproteinases-1 transgene. The Journal of cell biology 195 8978831
1988 Amino acid sequence and domain structure of entactin. Homology with epidermal growth factor precursor and low density lipoprotein receptor. The Journal of cell biology 149 3264556
1992 The receptor for the basement membrane glycoprotein entactin is the integrin alpha 3/beta 1. The Journal of biological chemistry 131 1527019
1981 Immunolocalization of entactin, a sulfated basement membrane component, in rodent tissues, and comparison with GP-2 (laminin). The American journal of pathology 131 6165248
1983 Immunohistochemical localization of entactin and laminin in mouse embryos and fetuses. Developmental biology 126 6653883
1993 Degradation of entactin by matrix metalloproteinases. Susceptibility to matrilysin and identification of cleavage sites. The Journal of biological chemistry 124 8380588
1992 Entactin stimulates neutrophil adhesion and chemotaxis through interactions between its Arg-Gly-Asp (RGD) domain and the leukocyte response integrin. The Journal of clinical investigation 120 1469085
2002 Neurologic defects and selective disruption of basement membranes in mice lacking entactin-1/nidogen-1. Laboratory investigation; a journal of technical methods and pathology 109 12480912
1983 Synthesis of laminin and entactin by F9 cells induced with retinoic acid and dibutyryl cyclic AMP. The Journal of biological chemistry 104 6305950
1990 The basement membrane glycoprotein entactin promotes cell attachment and binds calcium ions. The Journal of biological chemistry 89 2191952
2020 BET protein inhibitor JQ1 downregulates chromatin accessibility and suppresses metastasis of gastric cancer via inactivating RUNX2/NID1 signaling. Oncogenesis 82 32157097
1989 Heterogenous distribution of type IV collagen, entactin, heparan sulfate proteoglycan, and laminin among renal basement membranes as demonstrated by quantitative immunocytochemistry. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 81 2723404
1990 Entactin: structure and function. American journal of respiratory cell and molecular biology 77 2119632
1983 Changes in the rate of laminin and entactin synthesis in F9 embryonal carcinoma cells treated with retinoic acid and cyclic amp. Developmental biology 73 6194034
1993 Recombinant entactin promotes mouse primary trophoblast cell adhesion and migration through the Arg-Gly-Asp (RGD) recognition sequence. The Journal of cell biology 72 8491783
1986 Distribution of fibronectin, laminin and entactin in the environment of migrating neural crest cells in early mouse embryos. Journal of embryology and experimental morphology 68 3519827
1991 The expression of the genes for entactin, laminin A, laminin B1 and laminin B2 in murine lens morphogenesis and eye development. Differentiation; research in biological diversity 67 1725162
1999 Transforming growth factor-beta1 stimulates the synthesis of basement membrane proteins laminin, collagen type IV and entactin in rat liver sinusoidal endothelial cells. Journal of hepatology 66 10551394
1998 Entactin-2: a new member of basement membrane protein with high homology to entactin/nidogen. Experimental cell research 60 9633511
1983 In vitro synthesis of laminin and entactin polypeptides. The Journal of biological chemistry 55 6189826
1995 Two distinct cell attachment sites in entactin are revealed by amino acid substitutions and deletion of the RGD sequence in the cysteine-rich epidermal growth factor repeat 2. The Journal of biological chemistry 54 7797588
2017 NID1, a new regulator of EMT required for metastasis and chemoresistance of ovarian cancer cells. Oncotarget 53 28416770
2011 Genome-wide association study identifies nidogen 1 (NID1) as a susceptibility locus to cutaneous nevi and melanoma risk. Human molecular genetics 45 21478494
1993 Is neuronal intestinal dysplasia (NID) a primary disease or a secondary phenomenon? European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift fur Kinderchirurgie 45 8218074
1994 Binding of the extracellular matrix component entactin to Candida albicans. Infection and immunity 44 7927722
2013 Mutations in extracellular matrix genes NID1 and LAMC1 cause autosomal dominant Dandy-Walker malformation and occipital cephaloceles. Human mutation 39 23674478
1993 Immunohistochemical localization of chondroitin sulfate, chondroitin sulfate proteoglycan, heparan sulfate proteoglycan, entactin, and laminin in basement membranes of postnatal developing and adult rat lungs. American journal of respiratory cell and molecular biology 37 8448015
1982 Localization of fibronectin, laminin-entactin, and entactin in Reichert's membrane by immunoelectron microscopy. The EMBO journal 37 7188248
1994 Interaction of enteropathogenic Yersinia enterocolitica with complex basement membranes and the extracellular matrix proteins collagen type IV, laminin-1 and -2, and nidogen/entactin. The Journal of biological chemistry 36 7961965
1993 Biological functions of entactin. Kidney international 36 8433553
1990 Characterization of the basement membrane glycoprotein entactin synthesized in a baculovirus expression system. The Journal of biological chemistry 35 2180961
1988 Analysis of the assembly of laminin and the laminin-entactin complex with laminin chain specific monoclonal and polyclonal antibodies. Biochemistry 35 3149508
2013 Transcriptome-wide analysis of TDP-43 binding small RNAs identifies miR-NID1 (miR-8485), a novel miRNA that represses NRXN1 expression. Genomics 34 23827811
1996 Domain-specific interactions between entactin and neutrophil integrins. G2 domain ligation of integrin alpha3beta1 and E domain ligation of the leukocyte response integrin signal for different responses. The Journal of biological chemistry 34 8940031
1991 Potential role of entactin in hemostasis. Specific interaction of entactin with fibrinogen A alpha and B beta chains. The Journal of biological chemistry 34 1680863
2021 Loss of enteric neuronal Ndrg4 promotes colorectal cancer via increased release of Nid1 and Fbln2. EMBO reports 31 33890711
2019 Paracrine Induction of Epithelial-Mesenchymal Transition Between Colorectal Cancer Cells and its Suppression by a p53/miR-192/215/NID1 Axis. Cellular and molecular gastroenterology and hepatology 31 30831320
1996 Entactin expression by rat lung and rat alveolar epithelial cells. American journal of respiratory cell and molecular biology 31 8845174
2019 Proteomic Profiling of Paired Interstitial Fluids Reveals Dysregulated Pathways and Salivary NID1 as a Biomarker of Oral Cavity Squamous Cell Carcinoma. Molecular & cellular proteomics : MCP 30 31315917
1992 Entactin promotes adhesion and long-term maintenance of cultured regenerated skeletal myotubes. Journal of cellular physiology 30 1734030
1990 Entactin: a possible auto-antigen in the pathogenesis of non-Goodpasture anti-GBM nephritis. Kidney international 29 2119467
2004 Auditory brainstem evoked responses in insulin-dependent (ID) and non-insulin-dependent (NID) diabetic subjects with normal hearing. International journal of audiology 27 14974625
1987 Carboxyl-terminal sequence of entactin deduced from a cDNA clone. Proceedings of the National Academy of Sciences of the United States of America 27 3470744
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