Affinage

LAMC1

Laminin subunit gamma-1 · UniProt P11047

Round 2 corrected
Length
1609 aa
Mass
177.6 kDa
Annotated
2026-04-28
105 papers in source corpus 28 papers cited in narrative 28 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

LAMC1 encodes laminin subunit gamma-1, the most widely expressed gamma chain of the heterotrimeric laminin family, and serves as the essential nucleating scaffold for basement membrane assembly; genetic knockout in mice abolishes laminin heterotrimer formation, prevents incorporation of collagen IV, nidogen, and perlecan into the extracellular matrix, and causes embryonic lethality at E5.5 due to failure of primitive endoderm differentiation (PMID:9885251, PMID:21421915). LAMC1 transcription is driven by Sp1 and SMAD4 via GC-rich promoter elements, while its protein output is post-transcriptionally tuned by multiple miRNAs (miR-29a/b/c, miR-22, miR-124, miR-506, miR-543) and by NUDT21-controlled alternative polyadenylation that modulates 3′UTR length and miRNA accessibility (PMID:9403717, PMID:24820027, PMID:37389756, PMID:34218518). Functionally, LAMC1 promotes cell migration, invasion, survival, and epithelial–mesenchymal transition across multiple tissue contexts through activation of FAK/PI3K/AKT, Akt–NF-κB–MMP9/14, and Wnt/β-catenin signaling, and preserves blood–brain barrier integrity by stabilizing tight-junction proteins ZO-1 and occludin (PMID:38609046, PMID:33782812, PMID:35315295, PMID:41534552). A LAMC1 mutation disrupting the nidogen-1 interaction surface has been identified in autosomal dominant Dandy–Walker malformation (PMID:23674478).

Mechanistic history

Synthesis pass · year-by-year structured walk · 9 steps
  1. 1994 High

    Establishing gene identity: consensus nomenclature formalized LAMC1 as the gene encoding the laminin gamma-1 chain (formerly B2), a constitutive subunit of most heterotrimeric laminin isoforms, resolving prior naming ambiguities.

    Evidence Community nomenclature based on prior biochemical characterization of laminin chain composition

    PMID:7921537

    Open questions at the time
    • Chain-specific functional contributions versus other gamma chains not yet delineated
  2. 1999 High

    Answering whether LAMC1 is required for basement membrane formation: knockout demonstrated that without gamma-1, no laminin heterotrimers assemble, no basement membranes form, and embryos die at E5.5 — establishing LAMC1 as the indispensable nucleation scaffold for BM biogenesis.

    Evidence Homologous recombination knockout in mouse ES cells; embryoid body culture; immunofluorescence for ECM components; in vivo embryo analysis

    PMID:9885251 PMID:9917858

    Open questions at the time
    • Whether any compensatory gamma chain can partially substitute in specific tissues
    • Mechanism by which trophectoderm forms without BM remains unresolved
  3. 1997 High

    Defining transcriptional control: Sp1 was identified as a direct transactivator of the LAMC1 promoter via GC-rich elements, explaining upregulation at the invasion front of hepatocellular carcinomas and linking transcriptional regulation to cancer biology.

    Evidence EMSA; Sp1 transfection in Drosophila SL2 cells; LAMC1 promoter deletion constructs; Northern blot; in situ hybridization in human HCC tissue

    PMID:8611150 PMID:9403717

    Open questions at the time
    • Identity of the ~60 kDa CTC-binding factor remains unconfirmed
    • Chromatin-level regulation (enhancers, epigenetic marks) largely unexplored at this stage
  4. 2012 Medium

    Demonstrating tissue-specific developmental roles: mutation of a critical binding site in Lamc1 disrupted pial basement membrane integrity and cortical lamination, establishing that specific LAMC1 interaction surfaces are required for radial glial attachment and neuronal migration in cortical development.

    Evidence Mouse genetic model with Lamc1 binding-site mutation; histological analysis; immunofluorescence for dystroglycan and radial glia markers

    PMID:22961762

    Open questions at the time
    • Precise receptor(s) mediating radial glial attachment to LAMC1-containing laminins not identified
    • Whether other laminin isoforms contribute redundantly to pial BM not tested
  5. 2013 Medium

    Linking LAMC1 to human brain malformation: structural modeling showed a LAMC1 mutation in a Dandy–Walker malformation family disrupts the nidogen-1 binding interface, connecting a specific protein–protein interaction to cerebellar development.

    Evidence Whole-exome sequencing; structural modeling of NID1-LAMC1 complex; protein interaction network analysis

    PMID:23674478

    Open questions at the time
    • No biochemical reconstitution demonstrating disrupted NID1 binding
    • No animal model recapitulating the human mutation phenotype
  6. 2014 Medium

    Establishing post-transcriptional regulation by miRNAs: miR-29a/b/c were shown to directly target the LAMC1 3′UTR, and silencing LAMC1 phenocopied miR-29 restoration in suppressing prostate cancer migration and invasion — opening an axis of miRNA-based LAMC1 regulation that was subsequently extended to miR-22, miR-124, miR-506, and miR-543.

    Evidence Luciferase reporter assays; siRNA knockdown; migration/invasion assays in prostate cancer cells; extended by miR-22 ChIP-seq and luciferase validation (2015), miR-124 ceRNA studies (2017), miR-543 dual-luciferase assays (2021), and NUDT21-APA studies (2023)

    PMID:24820027 PMID:26052614 PMID:28524360 PMID:33782812 PMID:37389756

    Open questions at the time
    • Quantitative contribution of each miRNA to LAMC1 protein levels in vivo is unknown
    • Whether miRNA regulation of LAMC1 is relevant in non-cancer contexts remains largely untested
  7. 2019 Medium

    Mapping downstream signaling: LAMC1 was placed upstream of PI3K/AKT in hepatocellular carcinoma, where it drives the Warburg effect through PTEN suppression and PKM2 upregulation — the first detailed pathway placement connecting LAMC1 to cancer cell metabolism.

    Evidence siRNA knockdown and overexpression; PI3K inhibitor LY294002; glucose/lactate measurements; Western blotting for PTEN, p-AKT, PKM2

    PMID:30755064

    Open questions at the time
    • How an extracellular matrix subunit activates intracellular PTEN/AKT not mechanistically resolved
    • No receptor identified mediating the signal
  8. 2021 Medium

    Revealing LAMC1 as a paracrine signaling hub: TGFβ1-induced LAMC1 in esophageal SCC not only activates Akt–NF-κB–MMP9/14 in tumor cells but also induces CXCL1 secretion that reprograms fibroblasts into inflammatory CAFs via CXCR2-pSTAT3, linking LAMC1 to tumor microenvironment remodeling.

    Evidence RNA-seq; ChIP; luciferase reporter; co-culture model; Western blotting; CXCL1 ELISA

    PMID:34218518

    Open questions at the time
    • Whether LAMC1's paracrine effects require its incorporation into a laminin trimer or act as a monomer is unresolved
    • Mechanism connecting LAMC1 to CXCL1 transcription not delineated
  9. 2024 Medium

    Establishing a neuroprotective role: recombinant LAMC1 attenuates neuronal apoptosis after subarachnoid hemorrhage through FAK/PI3K/AKT, while astrocyte-derived LAMC1 preserves blood–brain barrier integrity by stabilizing ZO-1 and occludin — extending LAMC1 function from structural ECM roles to active signaling in neurological injury.

    Evidence Rat SAH model with intranasal rLAMC1; FAK/PI3K inhibitors; AAV overexpression in mouse ICH model; Evans Blue extravasation; Western blotting for tight junction proteins

    PMID:38609046 PMID:41534552

    Open questions at the time
    • Receptor through which LAMC1 activates FAK in neurons not identified
    • Whether LAMC1 acts as an assembled laminin trimer or free subunit in these injury models is unclear
    • Therapeutic window and translational relevance in human stroke unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Major unresolved question: the cell-surface receptor(s) mediating LAMC1-dependent intracellular signaling (FAK, PI3K/AKT, Wnt/β-catenin) have not been definitively identified, and whether signaling-competent LAMC1 must be assembled into a laminin heterotrimer or can function as a free chain remains mechanistically open.
  • No receptor identification for LAMC1-driven intracellular signaling
  • No structural data on LAMC1 alone versus in assembled trimer context
  • In vivo tissue-specific contribution of LAMC1 to individual laminin isoform functions not systematically resolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0098631 cell adhesion mediator activity 3
Localization
GO:0031012 extracellular matrix 4 GO:0005576 extracellular region 3
Pathway
R-HSA-1474244 Extracellular matrix organization 3 R-HSA-162582 Signal Transduction 3 R-HSA-1266738 Developmental Biology 2
Partners
COL4A1HNRNPCNID1NUDT21SMAD4SP1
Complex memberships
Laminin heterotrimer (alpha-beta-gamma)

Evidence

Reading pass · 28 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1999 Homologous recombination knockout of LAMC1 (laminin gamma1) in mouse embryonic stem cells demonstrated that the gamma1 subunit is necessary for laminin heterotrimer assembly; without LAMC1, no basement membranes form, and collagen IV, perlecan, and nidogen are not incorporated into extracellular matrix. Embryos die at E5.5 due to failure of primitive endoderm differentiation, while the first epithelium (trophectoderm) can form independently of basement membrane. Homologous recombination knockout in mouse ES cells; embryoid body culture; immunofluorescence; ECM protein localization assays The Journal of cell biology High 9885251 9917858
1997 The transcription factor Sp1 directly transactivates the LAMC1 promoter via GC-rich elements; Sp1 overexpression in normal hepatocytes increases laminin gamma1 mRNA, and co-expression of Sp1 and laminin gamma1 is observed in human hepatocellular carcinomas at the invasion front. DNase I hypersensitive site mapping; gel-shift (EMSA) assays; Sp1 expression vector transfection; co-transfection of LAMC1 promoter deletion constructs in Drosophila SL2 cells; Northern blot; in situ hybridization The American journal of pathology High 9403717
1996 Regulatory elements controlling LAMC1 expression in hepatocytes are located between -594 bp and -94 bp of the promoter and contain GC- and CTC-containing motifs; a ~60 kDa protein binds the CTC motif and is implicated in transcriptional activation of LAMC1 in hepatoma cells and cultured hepatocytes. Transfection of LAMC1 promoter deletion mutants; actinomycin D/cycloheximide treatment; gel-shift assay; affinity chromatography; Southwestern analysis The Biochemical journal Medium 8611150
2013 Structural modeling of the NID1-LAMC1 complex showed that a LAMC1 mutation identified in a family with autosomal dominant Dandy-Walker malformation disrupts the NID1-LAMC1 protein-protein interaction, implicating this ECM interaction in cerebellar development. Whole-exome sequencing; protein interaction network analysis; structural modeling of NID1-LAMC1 complex Human mutation Medium 23674478
2014 miR-29s (miR-29a/b/c) directly target the 3'UTR of LAMC1, as confirmed by luciferase reporter assays; restoration of miR-29s in prostate cancer cells inhibits cell migration and invasion, and silencing of LAMC1 phenocopies this effect, placing LAMC1 downstream of miR-29s in the focal adhesion pathway governing prostate cancer metastasis. Luciferase reporter assays; siRNA knockdown of LAMC1; migration/invasion assays; gene expression profiling; in silico target prediction International journal of oncology Medium 24820027
2015 miR-22 and miR-29a are directly regulated by the androgen receptor (AR) via AR binding sites identified by ChIP-seq; miR-22 directly targets LAMC1 (validated by luciferase reporter), and transfection of miR-22 mimics in prostate cancer cells reduces LAMC1 expression, induces apoptosis, and diminishes cell migration. ChIP-seq; microarray expression profiling; luciferase reporter assay; miRNA mimic transfection; apoptosis and migration assays Molecular endocrinology (Baltimore, Md.) Medium 26052614
2019 LAMC1 promotes the Warburg effect in hepatocellular carcinoma cells by upregulating PKM2 expression through the PTEN/AKT signaling pathway; LAMC1 knockdown reduces glucose consumption and lactate production, decreases GLUT1 and LDHA expression, increases PTEN, and reduces AKT phosphorylation, while PI3K inhibition blocks LAMC1-induced PKM2 upregulation. siRNA knockdown and overexpression of LAMC1; PI3K inhibitor (LY294002); glucose/lactate measurement; Western blotting for GLUT1, LDHA, PTEN, p-AKT, PKM2 Cancer biology & therapy Medium 30755064
2021 TGFβ1 upregulates LAMC1 in esophageal squamous cell carcinoma cells through synergistic activation by SMAD4 and SP1; LAMC1 in turn promotes tumor cell proliferation and migration via Akt-NFκB-MMP9/14 signaling, and induces CXCL1 secretion that stimulates formation of inflammatory cancer-associated fibroblasts (iCAF) through CXCR2-pSTAT3. RNA sequencing; ChIP; luciferase reporter assay; siRNA knockdown; co-culture model; Western blotting; ELISA for CXCL1 Molecular oncology Medium 34218518
2022 LAMC1 secreted by gastric cancer cells in an autocrine manner promotes preadipocyte maturation and rupture to release free fatty acids (FFAs) in peritoneal microenvironment; palmitic acid (a FFA) activates p-STAT3, which binds the miR-193a-3p promoter to inhibit miR-193a-3p, thereby de-repressing LAMC1 in a post-translational regulatory loop; single-molecular magnetic tweezers confirmed the STAT3-promoter binding is stable, reversible, and ATP-dependent. Co-culture model; luciferase reporter; RNA immunoprecipitation (RIP); ChIP; single-molecular magnetic tweezers; immunohistochemical staining; single-cell sequencing International journal of biological sciences Medium 35541892
2012 Mutation of a critical binding site in Lamc1 (laminin gamma1) disrupts cortical lamination in mice, implicating LAMC1-containing laminins as ECM ligands required for pial basement membrane integrity, radial glial cell attachment, and cortical neuron migration. Mouse genetic model (Lamc1 binding-site mutation); histological analysis of cortical layers; immunofluorescence for dystroglycan and radial glia markers Developmental neurobiology Medium 22961762
2006 A sequence variant (rs10911193) in the LAMC1 promoter disrupts the binding site for transcription factor NFIL3; the rare T allele segregates with early-onset pelvic organ prolapse in affected families, and NFIL3 was verified to be co-expressed with LAMC1 in vaginal tissue, suggesting NFIL3-driven LAMC1 regulation contributes to connective tissue integrity. Genome-wide linkage scan; RT-PCR; SNP genotyping; promoter sequence analysis identifying NFIL3 binding site Human genetics Low 17021862
2017 LAMC1 mRNA acts as a competing endogenous RNA (ceRNA) for miR-124 in hepatocellular carcinoma; miR-124 negatively regulates LAMC1 through two binding sites in its 3'UTR, and the wild-type LAMC1 miRNA response elements (MREs) competitively sponge miR-124 to facilitate CD151 expression, thereby promoting HCC cell migration and invasion. Luciferase reporter assay with 3'UTR constructs; miR-124 overexpression; siRNA knockdown; qRT-PCR; correlation analysis in clinical tissues IUBMB life Medium 28524360
2021 LncRNA SNHG6 acts as a sponge for miR-543 to disinhibit LAMC1; miR-543 directly targets LAMC1 (validated by dual-luciferase assay), and LAMC1 promotes breast cancer cell proliferation, migration, invasion, and EMT via activation of the PI3K/AKT pathway; overexpression of LAMC1 reverses the tumor-suppressive effects of miR-543. Dual-luciferase reporter assay; siRNA knockdown; colony formation; flow cytometry; wound healing; transwell assay; xenograft model; Western blotting for PI3K/AKT pathway Breast cancer research and treatment Medium 33782812
2022 Exosomal miR-424-5p from ER-stressed head and neck squamous cell carcinoma cells directly targets LAMC1 (validated by luciferase reporter and Western blot), inhibiting angiogenesis and migration of HUVECs through repression of the LAMC1-mediated Wnt/β-catenin signaling pathway. Exosome isolation/characterization; RNA-seq; matrigel tube formation assay; transwell assay; luciferase reporter assay; Western blotting Cell transplantation Medium 35315295
2024 LAMC1 attenuates neuronal apoptosis after subarachnoid hemorrhage via the FAK/PI3K/AKT signaling pathway; recombinant LAMC1 increases p-FAK, p-PI3K, p-AKT, Bcl-XL, and Bcl-2 and decreases Bax and cleaved caspase-3; knockdown of LAMC1 worsens neurological deficits; pharmacological inhibition of FAK (Y15) or PI3K (LY294002) abolishes rLAMC1's protective effects. Rat endovascular perforation SAH model; intranasal rLAMC1 administration; siRNA knockdown; FAK inhibitor Y15; PI3K inhibitor LY294002; Western blotting; TUNEL staining; immunofluorescence; neurobehavioral testing; in vitro oxyhemoglobin model Experimental neurology Medium 38609046
2023 NUDT21-mediated alternative polyadenylation (APA) regulates LAMC1 expression in glioma; NUDT21 knockdown causes preferential use of the proximal polyA signal of LAMC1, shortening the 3'UTR and removing miR-124/506 binding sites, thereby increasing LAMC1 protein via relief of miRNA-based repression; co-depletion of LAMC1 abolishes the pro-migratory effect of NUDT21 knockdown. PAC-seq; PolyAMiner bioinformatic pipeline; qRT-PCR; Western blotting; glioma cell migration assays; NUDT21 and LAMC1 knockdown Journal of neuro-oncology Medium 37389756
2020 miR-183 inhibits embryo implantation in mice by binding directly to Lamc1 (laminin gamma1) and Hbegf mRNAs; in vivo uterine delivery of miR-183 agomir reduces implantation sites, and loss/gain of miR-183 function in endometrial cells shows inhibition of cell migration, invasion, and apoptosis, placing Lamc1 as a key target of miR-183 in uterine receptivity. In vivo miRNA agomir treatment; in vitro gain/loss of function; identification of miR-183 binding to Lamc1; mouse implantation model Theriogenology Low 32980684
2022 LAMC1 intronic variant rs3768617 associated with Fuchs endothelial corneal dystrophy shows allele-specific binding of transcription factor GFI1B to the risk allele 'G' (but not protective allele 'A'), as validated by EMSA and ChIP assay; luciferase assay confirmed the locus has regulatory activity; GFI1B expression is reduced in FECD-affected corneal endothelium. Genotyping; luciferase assay; EMSA; ChIP assay; immunofluorescence; bioinformatics Gene Medium 35031421
2024 In hyperglycemic endothelial cells, protein disulfide isomerase A1 (PDI) regulates secretion of LAMC1; siRNA knockdown of LAMC1 decreased platelet-endothelium interaction and did not potentiate PDI inhibitor effects, establishing LAMC1 as a downstream effector of PDI-dependent pathways controlling endothelial membrane stiffness and platelet adhesion. Secretomics (proteomics of conditioned medium); siRNA knockdown; immunofluorescence; atomic force microscopy; transendothelial resistance assay; PDI inhibitors Journal of thrombosis and haemostasis Medium 39128656
2025 Astrocyte-derived LAMC1 preserves blood-brain barrier integrity after intracerebral hemorrhage by inhibiting degradation of tight junction proteins ZO-1 and occludin; AAV-mediated LAMC1 overexpression in mice reduced brain edema, tissue necrosis, and improved neuromotor function post-ICH; single-cell eQTL analysis pinpointed astrocytes as the key cell type driving LAMC1's protective effects. Multi-omics integration (TWAS, PWAS); single-cell eQTL; AAV gain-of-function in vivo; Evans Blue extravasation; brain edema measurement; Western blotting for ZO-1, occludin; neurobehavioral testing Journal of advanced research Medium 41534552
2025 LAMC1 knockdown in lung adenocarcinoma cell lines decreases radioresistance, affecting proliferation and apoptosis following irradiation; LAMC1 protein levels in exosomes decrease following radiotherapy, identifying it as a functional determinant of radiation resistance. DIA-mass spectrometry proteomics; siRNA knockdown; surviving fraction at 2 Gy assay; nano-flow cytometry for exosomal LAMC1 Current pharmaceutical design Low 40326033
2025 LAMC1 promotes epithelial-mesenchymal transition (EMT) and retinal pigment epithelial cell migration/invasion in diabetic retinopathy via activation of the PI3K/AKT signaling pathway; LAMC1 is upregulated in high-glucose-treated ARPE-19 cells and diabetic mouse retinas, and LAMC1 knockdown alleviates retinal damage in diabetic mice. GEO microarray dataset analysis; Western blotting; in vitro high-glucose model; siRNA knockdown; in vivo diabetic mouse model; EMT marker analysis The journal of physiological sciences Low 41092639
2024 Lamc1 promotes osteogenic differentiation and inhibits adipogenic differentiation of bone marrow-derived mesenchymal stem cells; knockdown of Lamc1 inhibits both adipogenic and osteogenic differentiation, and cell communication analysis identified two receptors interacting with LAMC1 in this context. Transcriptomic analysis of public datasets; single-cell RNA-seq validation; q-PCR; siRNA knockdown of Lamc1 Scientific reports Low 39179716
2025 Live imaging of endogenous zebrafish Lamc1 tagged at the C-terminus by in-fusion genome editing showed that Lamc1 is stable in fin fold matrix but more dynamic (as measured by FRAP) in myoseptal matrix, revealing tissue-specific turnover dynamics of LAMC1 in vivo. C-terminus in-fusion genome editing (knock-in); confocal live-cell imaging; FRAP analysis in developing zebrafish bioRxivpreprint Medium
1994 LAMC1 was established as the gene encoding the laminin gamma1 chain (previously called B2 chain), which together with alpha and beta chains forms the heterotrimeric laminin molecule; the new nomenclature formalized the gene symbol LAMC1 for this subunit. Nomenclature consensus based on biochemical and molecular characterization of laminin chain composition Matrix biology High 7921537
1989 S-laminin (encoded by LAMB2, but establishing the paradigm for laminin chain-specific synaptic localization) was identified as a glycoprotein selectively concentrated in synaptic basal lamina that is recognized by motoneurons and promotes synaptic specificity — establishing the principle that individual laminin chains confer isoform-specific tissue localization. Biochemical isolation; immunohistochemistry; molecular cloning Nature High 2922051
2011 Basement membranes are self-assembled on cell surfaces through binding interactions among laminins (including gamma1-containing isoforms), type IV collagens, nidogens, and proteoglycans; laminins serve as the nucleating scaffold for this assembly, with gamma1 being present in most laminin isoforms and essential for this organization. Review synthesizing biochemical reconstitution and genetic studies of basement membrane assembly Cold Spring Harbor perspectives in biology High 21421915
2024 LncRNA TM4SF19-AS1 upregulates LAMC1 expression in head and neck squamous cell carcinoma by two mechanisms: (1) sequestering miR-153-3p (which targets LAMC1) as a ceRNA sponge, and (2) recruiting HNRNPC protein to enhance LAMC1 expression; knockdown of TM4SF19-AS1 reduces LAMC1 and inhibits HNSCC proliferation, migration, invasion, and EMT. RT-qPCR; Western blotting; luciferase reporter assay; RNA immunoprecipitation for HNRNPC; siRNA knockdown; in vivo tumor assays Cancer biology & therapy Medium 36411963

Source papers

Stage 0 corpus · 105 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2019 Blood-Brain Barrier: From Physiology to Disease and Back. Physiological reviews 1645 30280653
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2003 Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. Nature biotechnology 1176 12754519
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2011 Basement membranes: cell scaffoldings and signaling platforms. Cold Spring Harbor perspectives in biology 720 21421915
1994 A new nomenclature for the laminins. Matrix biology : journal of the International Society for Matrix Biology 716 7921537
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2008 Large-scale proteomics and phosphoproteomics of urinary exosomes. Journal of the American Society of Nephrology : JASN 607 19056867
1989 A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction. Nature 550 2922051
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
1999 Absence of basement membranes after targeting the LAMC1 gene results in embryonic lethality due to failure of endoderm differentiation. The Journal of cell biology 432 9885251
1987 Laminin-nidogen complex. Extraction with chelating agents and structural characterization. European journal of biochemistry 423 3109910
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
1996 Normalization and subtraction: two approaches to facilitate gene discovery. Genome research 401 8889548
2005 Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of proteome research 350 16335952
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2001 Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling. Journal of cell science 333 11683410
2007 Nephrotic syndrome in the first year of life: two thirds of cases are caused by mutations in 4 genes (NPHS1, NPHS2, WT1, and LAMB2). Pediatrics 313 17371932
2012 Functional diversity of laminins. Annual review of cell and developmental biology 298 23057746
2020 The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis. Nature 292 32322062
2012 Identification of Genetic Susceptibility Loci for Colorectal Tumors in a Genome-Wide Meta-analysis. Gastroenterology 283 23266556
2000 Integrins as receptors for laminins. Microscopy research and technique 278 11054877
2004 Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. Nature biotechnology 266 15146197
2011 A directed protein interaction network for investigating intracellular signal transduction. Science signaling 258 21900206
2004 Functional proteomics mapping of a human signaling pathway. Genome research 247 15231748
2009 Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Journal of proteome research 237 19199708
2010 Mutations in the human laminin beta2 (LAMB2) gene and the associated phenotypic spectrum. Human mutation 155 20556798
2006 Recessive missense mutations in LAMB2 expand the clinical spectrum of LAMB2-associated disorders. Kidney international 136 16912710
2009 Mutations in LAMB2 causing a severe form of synaptic congenital myasthenic syndrome. Journal of medical genetics 97 19251977
2014 Tumor-suppressive microRNA-29s inhibit cancer cell migration and invasion via targeting LAMC1 in prostate cancer. International journal of oncology 94 24820027
2015 miR-22 and miR-29a Are Members of the Androgen Receptor Cistrome Modulating LAMC1 and Mcl-1 in Prostate Cancer. Molecular endocrinology (Baltimore, Md.) 69 26052614
2012 β2 and γ3 laminins are critical cortical basement membrane components: ablation of Lamb2 and Lamc3 genes disrupts cortical lamination and produces dysplasia. Developmental neurobiology 68 22961762
2006 Sequence variant in the laminin gamma1 (LAMC1) gene associated with familial pelvic organ prolapse. Human genetics 62 17021862
2017 A functional polymorphism located at transcription factor binding sites, rs6695837 near LAMC1 gene, confers risk of colorectal cancer in Chinese populations. Carcinogenesis 61 28039327
2019 Lamc1 promotes the Warburg effect in hepatocellular carcinoma cells by regulating PKM2 expression through AKT pathway. Cancer biology & therapy 57 30755064
2021 LAMC1 upregulation via TGFβ induces inflammatory cancer-associated fibroblasts in esophageal squamous cell carcinoma via NF-κB-CXCL1-STAT3. Molecular oncology 51 34218518
2011 A missense LAMB2 mutation causes congenital nephrotic syndrome by impairing laminin secretion. Journal of the American Society of Nephrology : JASN 46 21511833
2006 A syndrome comprising childhood-onset glomerular kidney disease and ocular abnormalities with progressive loss of vision is caused by mutated LAMB2. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 45 16921188
1998 The targeted deletion of the LAMC1 gene. Annals of the New York Academy of Sciences 44 9917858
1995 The human laminin beta 2 chain (S-laminin): structure, expression in fetal tissues and chromosomal assignment of the LAMB2 gene. Matrix biology : journal of the International Society for Matrix Biology 42 7795887
1997 Sp1-mediated transactivation of LamC1 promoter and coordinated expression of laminin-gamma1 and Sp1 in human hepatocellular carcinomas. The American journal of pathology 41 9403717
2013 Mutations in extracellular matrix genes NID1 and LAMC1 cause autosomal dominant Dandy-Walker malformation and occipital cephaloceles. Human mutation 39 23674478
1996 Isolation of a novel gene from the DiGeorge syndrome critical region with homology to Drosophila gdl and to human LAMC1 genes. Human molecular genetics 39 8733130
2022 LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis. International journal of biological sciences 36 35541892
2011 A novel mutation of LAMB2 in a multigenerational mennonite family reveals a new phenotypic variant of Pierson syndrome. Ophthalmology 29 21236492
2021 LncRNA SNHG6 promotes breast cancer progression and epithelial-mesenchymal transition via miR-543/LAMC1 axis. Breast cancer research and treatment 27 33782812
1988 Isolation of a human laminin B2 (LAMB2) cDNA clone and assignment of the gene to chromosome region 1q25----q31. Cytogenetics and cell genetics 25 3234037
2022 PSMA3-AS1 induced by transcription factor PAX5 promotes cholangiocarcinoma proliferation, migration and invasion by sponging miR-376a-3p to up-regulate LAMC1. Aging 24 35022330
2012 LAMC1 gene is associated with premature ovarian failure. Maturitas 24 22321639
2016 MicroRNA-506 Inhibits Malignancy of Colorectal Carcinoma Cells by Targeting LAMC1. Annals of clinical and laboratory science 23 27993882
2022 The Endoplasmic Reticulum-Stressed Head and Neck Squamous Cell Carcinoma Cells Induced Exosomal miR-424-5p Inhibits Angiogenesis and Migration of Humanumbilical Vein Endothelial Cells Through LAMC1-Mediated Wnt/β-Catenin Signaling Pathway. Cell transplantation 21 35315295
2012 Comprehensive analysis of LAMC1 genetic variants in advanced pelvic organ prolapse. American journal of obstetrics and gynecology 18 22342894
2020 Long noncoding RNA Airn protects podocytes from diabetic nephropathy lesions via binding to Igf2bp2 and facilitating translation of Igf2 and Lamb2. Cell biology international 17 32437062
2017 LAMC1 mRNA promotes malignancy of hepatocellular carcinoma cells by competing for MicroRNA-124 binding with CD151. IUBMB life 17 28524360
2009 The first Chinese Pierson syndrome with novel mutations in LAMB2. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 16 19861315
2024 LAMC1 attenuates neuronal apoptosis via FAK/PI3K/AKT signaling pathway after subarachnoid hemorrhage. Experimental neurology 13 38609046
2022 Astrocytes promote the proliferation of oligodendrocyte precursor cells through connexin 47-mediated LAMB2 secretion in exosomes. Molecular biology reports 13 35596050
2019 miR-506 contributes to malignancy of cutaneous squamous cell carcinoma via targeting of P65 and LAMC1. Cell cycle (Georgetown, Tex.) 12 30646812
2014 Retrospective mutational analysis of NPHS1, NPHS2, WT1 and LAMB2 in children with steroid-resistant focal segmental glomerulosclerosis - a single-centre experience. Bosnian journal of basic medical sciences 12 24856380
2016 A novel LAMB2 gene mutation associated with a severe phenotype in a neonate with Pierson syndrome. European journal of medical research 11 27130041
2018 Screening of the LAMB2, WT1, NPHS1, and NPHS2 Genes in Pediatric Nephrotic Syndrome. Frontiers in genetics 9 30013592
2017 A novel mutation of laminin β2 (LAMB2) in two siblings with renal failure. European journal of pediatrics 9 28188379
1996 Expression of laminin gamma 1 cultured hepatocytes involves repeated CTC and GC elements in the LAMC1 promoter. The Biochemical journal 9 8611150
2024 Hypoxia-induced LAMB2-enriched extracellular vesicles promote peritoneal metastasis in gastric cancer via the ROCK1-CAV1-Rab11 axis. Oncogene 8 39138263
2020 Mutations in LAMB2 Are Associated With Albuminuria and Optic Nerve Hypoplasia With Hypopituitarism. The Journal of clinical endocrinology and metabolism 8 31769495
2020 MiR-183 impeded embryo implantation by regulating Hbegf and Lamc1 in mouse uterus. Theriogenology 8 32980684
2016 Simultaneous mutations of LAMB2 and NPHP1genes in a Chinese girl with isolated congenital nephrotic syndrome: a case report. BMC pediatrics 8 27004562
1991 The gene for retinal rod 33-kDa protein is on mouse chromosome 1, near Lamb2. Cytogenetics and cell genetics 8 1675982
2023 NUDT21 alters glioma migration through differential alternative polyadenylation of LAMC1. Journal of neuro-oncology 7 37389756
2022 LncRNA TM4SF19-AS1 exacerbates cell proliferation, migration, invasion, and EMT in head and neck squamous cell carcinoma via enhancing LAMC1 expression. Cancer biology & therapy 7 36411963
2020 Common variants in LAMC1 confer risk for pelvic organ prolapse in Chinese population. Hereditas 7 32635941
2017 Nephron development and extrarenal features in a child with congenital nephrotic syndrome caused by null LAMB2 mutations. BMC nephrology 7 28683731
2022 The long non-coding RNA ASMTL-AS1 promotes hepatocellular carcinoma progression by sponging miR-1343-3p that suppresses LAMC1 (laminin subunit gamma 1). Bioengineered 6 34859735
2024 Lamc1 promotes osteogenic differentiation and inhibits adipogenic differentiation of bone marrow-derived mesenchymal stem cells. Scientific reports 5 39179716
2024 Ginsenoside Rg1 Regulates the Activation of Astrocytes Through lncRNA-Malat1/miR-124-3p/Lamc1 Axis Driving PI3K/AKT Signaling Pathway, Promoting the Repair of Spinal Cord Injury. CNS neuroscience & therapeutics 5 39491316
2022 Association of miRNA targetome variants in LAMC1 and GNB3 genes with colorectal cancer and obesity. Cancer medicine 5 35373932
2017 LAMB2 mutation with different phenotypes in China
. Clinical nephrology 5 27925579
2025 Serum Exosomal miR-216a Contributes to Acute Pancreatitis-Associated Acute Lung Injury by Enhancing Endothelial Cell Vascular Permeability Through Downregulating LAMC1. Pancreas 4 39937877
2019 A Novel Homozygous Truncating Mutation in LAMB2 Gene in a Chinese Uyghur Patient With Severe Phenotype Pierson Syndrome. Frontiers in medicine 4 30778388
2014 Next-generation sequencing to dissect hereditary nephrotic syndrome in mice identifies a hypomorphic mutation in Lamb2 and models Pierson's syndrome. The Journal of pathology 4 24293254
1999 Characterization of the human laminin beta2 chain locus (LAMB2): linkage to a gene containing a nonprocessed, transcribed LAMB2-like pseudogene (LAMB2L) and to the gene encoding glutaminyl tRNA synthetase (QARS). Cytogenetics and cell genetics 4 10393422
2024 Endothelial protein disulfide isomerase A1 enhances membrane stiffness and platelet-endothelium interaction in hyperglycemia via SLC3A2 and LAMC1. Journal of thrombosis and haemostasis : JTH 3 39128656
2023 Circ_0124208 Promotes the Progression of Hepatocellular Carcinoma by Regulating the miR-338-3p/LAMC1 Axis. Molecular biotechnology 3 36780058
2023 Hsa_circ_0005085 may suppress cutaneous squamous cell carcinoma growth and metastasis through targeting the miR-186-5p/LAMC1 axis. Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 3 37357644
2023 Early-Onset Myopia and Retinal Detachment without Typical Microcoria or Severe Proteinuria due to a Novel LAMB2 Variant. Ophthalmology. Retina 3 37678612
2022 Fuchs Endothelial Corneal Dystrophy associated risk variant, rs3768617 in LAMC1 shows allele specific binding of GFI1B. Gene 3 35031421
2021 Mutational landscape of TRPC6, WT1, LMX1B, APOL1, PTPRO, PMM2, LAMB2 and WT1 genes associated with Steroid resistant nephrotic syndrome. Molecular biology reports 3 34546508
2006 [LAMB2 gene mutation as a cause of congenital nephrotic syndrome with distinct eye abnormalities and hypotonia]. Przeglad lekarski 3 16898484
2023 LAMB2 gene: broad clinical spectrum in Pierson syndrome. CEN case reports 2 38038886
2022 Whole exome sequencing identifies KIF26B, LIFR and LAMC1 mutations in familial vesicoureteral reflux. PloS one 2 36417404
2025 How does LAMB2 contribute to kidney disease? Insights from a pediatric case. BMC nephrology 1 40826341
2024 Expanding the spectrum of LAMB2: Pierson syndrome associated with neuromuscular junction disorder in two patients. Neuromuscular disorders : NMD 1 38723581
2024 The clinical characteristics and genotype analysis of LAMB2 gene mutation. Frontiers in medicine 1 39416865
2023 A novel missense variant in LAMC1 identified in a POI family by whole exome sequencing. Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology 1 37839437
2021 An extremely mild clinical course in a case with LAMB2-associated nephritis diagnosed with next-generation sequencing. CEN case reports 1 33476040
2026 Astrocyte-derived LAMC1 protects against intracerebral hemorrhage: A novel genetic mechanism maintaining neurovascular integrity. Journal of advanced research 0 41534552
2025 Data-independent Acquisition Mass Spectrometry Reveals Exosomal LAMC1 as a Key Determinant of Lung Adenocarcinoma Radiosensitivity, Independent of EGFR Mutation. Current pharmaceutical design 0 40326033
2025 LAMC1 aggravates diabetic retinopathy through PI3K/AKT signaling-regulated epithelial-mesenchymal transition in retinal pigment epithelial cells. The journal of physiological sciences : JPS 0 41092639
2021 LAMB2 novel variant c.2885-9 C>A affects RNA splicing in a minigene assay. Molecular genetics & genomic medicine 0 33982833