Affinage

MCM10

Protein MCM10 homolog · UniProt Q7L590

Length
875 aa
Mass
98.2 kDa
Annotated
2026-06-10
100 papers in source corpus 58 papers cited in narrative 59 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 10/10 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MCM10 is a conserved, essential nuclear scaffold protein that acts at the transition from replication licensing to origin firing, functioning after pre-replicative complex assembly but before origin DNA unwinding (PMID:1514326, PMID:11864598, PMID:12972571). It is recruited to origins through direct contacts between its C-terminus and the inactive MCM2-7 double hexamer (engaging Mcm2 and Mcm6), with low-affinity binding before and high-affinity binding after CMG assembly (PMID:26719337, PMID:26686640, PMID:11095689). Once bound, MCM10 facilitates Cdc45 recruitment and CMG formation, stabilizes Cdc45-GINS association with MCM2-7, and stimulates DDK-mediated phosphorylation of Mcm2 (PMID:15201046, PMID:26582917, PMID:19805216, PMID:28270517). Acute-depletion studies established that its defining role is to drive the origin-unwinding step after a stable CMG has assembled: MCM10 loss permits CMG assembly but blocks DNA melting, CMG translocation, and RPA loading (PMID:22433841, PMID:22433840, PMID:22285032). Structurally, MCM10 is a self-associating protein whose internal (ID) and C-terminal (CTD) domains use OB-folds and zinc motifs to bind ssDNA, dsDNA, and the p180 catalytic subunit of DNA polymerase-alpha, supporting a handoff that loads pol-alpha at origins, and its ssDNA-binding activity is independently required for origin melting (PMID:19081065, PMID:18065420, PMID:19608746, PMID:27751725, PMID:12844493, PMID:23894664). MCM10 acts as a chaperone that stabilizes pol-alpha (p180/Cdc17) against degradation across yeast, Xenopus, and human cells, and it bridges the replisome to And-1/Ctf4 and RECQ4 to complete helicase activation (PMID:16675460, PMID:17699597, PMID:17761813, PMID:19696745, PMID:40799587). During elongation MCM10 binds the N-face of CMG via multiple subunit contacts, stimulates helicase activity and replisome processivity, isomerizes CMG from duplex-encircling to strand-exclusion mode, and through strand-annealing activity inhibits SMARCAL1-mediated fork regression (PMID:28869037, PMID:30598452, PMID:38967018, PMID:28270517). It also engages PCNA via a PIP box (only the diubiquitinated form binds) and partners with BRCA2 to suppress PRIMPOL-mediated repriming, linking it to lagging-strand synthesis and replication-stress tolerance (PMID:16782870, PMID:34645815). MCM10 levels and activity are tightly controlled by cell-cycle-dependent proteolysis, phosphorylation, SIRT1-mediated deacetylation, SUMOylation, and CRL4-DDB1-VprBP-mediated ubiquitination, including UV-induced and HIV-1 Vpr-driven degradation (PMID:11602595, PMID:23449222, PMID:34185429, PMID:22570418, PMID:26032416). MCM10 deficiency causes chronic replication stress, genomic instability, and telomere erosion through accumulation of aberrant fork structures (PMID:33712616).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1992 High

    Established MCM10 as an essential gene whose loss blocks S-phase progression, defining it as a core DNA replication factor before any molecular function was known.

    Evidence Temperature-sensitive mutant screen and complementation cloning in S. cerevisiae

    PMID:1514326

    Open questions at the time
    • No molecular function assigned
    • No interaction partners identified
  2. 1997 High

    Connected MCM10 physically and functionally to the MCM2-7 family, showing it is required both for initiation at origins and for fork progression through them.

    Evidence 2D DNA gel analysis, physical interaction and genetic assays in S. cerevisiae

    PMID:9154825

    Open questions at the time
    • Specific MCM subunit contacts not mapped
    • Mechanism of fork pausing unknown
  3. 2000 High

    Localized MCM10 function to origin loading of MCM2-7 via direct Mcm7 interaction, and identified conserved human interactions with Orc2, Mcm2, and Mcm6.

    Evidence Genetic epistasis, physical interaction and chromatin assays in S. cerevisiae; co-IP and two-hybrid in human cells

    PMID:10783164 PMID:11095689

    Open questions at the time
    • Whether MCM10 acts before or after pre-RC formation unresolved at this stage
    • Human interaction data from single lab
  4. 2003 High

    Placed MCM10 epistatically between pre-RC formation and Cdc45 loading and revealed a role in DDK-dependent MCM phosphorylation, refining its temporal position in initiation.

    Evidence Degron inactivation and chromatin assays in S. pombe; in vitro kinase assay with Dfp1-Hsk1 and truncation mapping; two-hybrid/RNAi in Drosophila

    PMID:12604790 PMID:12808023 PMID:12972571

    Open questions at the time
    • Direct demonstration that MCM10 stimulates unwinding not yet available
    • Kinase-stimulation mechanism unresolved
  5. 2004 Medium

    Defined two distinct activities: facilitating Cdc45 recruitment to origins and stabilizing the pol-alpha catalytic subunit against degradation.

    Evidence ChIP, co-IP, overexpression suppression and conditional degron in S. cerevisiae

    PMID:15201046 PMID:15494305

    Open questions at the time
    • Molecular basis of pol-alpha stabilization not yet defined
    • Cdc45 interaction surface unmapped
  6. 2006 High

    Assigned the pol-alpha-stabilizing activity to a conserved Hsp10-like chaperone domain and linked MCM10 to PCNA via a PIP box dependent on diubiquitination.

    Evidence Domain mutagenesis with rescue and PIP-box mutational analysis with genetic rescue in S. cerevisiae

    PMID:16675460 PMID:16782870

    Open questions at the time
    • Identity of the ubiquitin ligase for MCM10 diubiquitination unknown
    • Functional consequence of PCNA binding for Okazaki processing not directly tested
  7. 2007 High

    Resolved MCM10 domain architecture and DNA-/pol-alpha-binding surfaces, and integrated MCM10 into the And-1/Ctf4 axis for pol-alpha chromatin loading.

    Evidence Crystallography, NMR and biochemistry of Xenopus Mcm10 domains; EM of human hexamer; co-IP and Xenopus extract depletion for And-1; siRNA in HeLa for human p180 stabilization

    PMID:17699597 PMID:17761813 PMID:17823614 PMID:18065420 PMID:19081065

    Open questions at the time
    • Hexameric EM structure lacks mutagenesis validation
    • Stoichiometry on DNA in vivo unclear
  8. 2009 High

    Established the structural handoff logic (ssDNA and pol-alpha compete for the ID OB-fold) and embedded MCM10 in CMG/RECQ4 helicase complex assembly in human cells.

    Evidence Crystallography of ID-ssDNA complex with competition assays; purified complex co-IP and in vitro unwinding for RECQ4; BiFC and siRNA for CMG formation; EMSA/SPR for cooperative DNA binding

    PMID:19605346 PMID:19608746 PMID:19696745 PMID:19805216

    Open questions at the time
    • Timing of pol-alpha handoff relative to unwinding unresolved
    • RECQ4-MCM10 functional hierarchy not yet ordered
  9. 2012 High

    Three independent acute-depletion studies converged on a discrete post-CMG-assembly function: MCM10 is required for origin DNA unwinding, CMG translocation, and RPA loading, not for CMG assembly itself.

    Evidence Auxin-inducible and off-AID degron systems with ChIP and 2D gels in S. cerevisiae and S. pombe

    PMID:22285032 PMID:22433840 PMID:22433841

    Open questions at the time
    • Biochemical mechanism of the unwinding step not yet reconstituted
    • Role of the zinc finger in RPA loading mechanistically unexplained
  10. 2015 High

    Mapped the MCM10-MCM2-7 interface to the C-terminus contacting Mcm2/Mcm6 (overlapping the Cdt1 site), distinguished low- versus high-affinity recruitment modes, and tied MCM10 to DDK stimulation and Cdc45/GINS stabilization.

    Evidence Pulldowns with purified MCM double/single hexamers, replication reconstitution, endogenous DH purification with fusion rescue, and in vitro DDK assays in S. cerevisiae; ChIP and siRNA for RecQL4-dependent recruitment in human cells

    PMID:25602958 PMID:26582917 PMID:26686640 PMID:26719337

    Open questions at the time
    • How MCM10 promotes double-hexamer remodeling structurally undefined
    • Order of RecQL4 and MCM10 origin loading still debated
  11. 2017 High

    Reconstitution demonstrated that MCM10 stimulates CMG helicase activity, supports elongation and isomerizes CMG to a strand-exclusion mode, with separation-of-function alleles uncoupling initial unwinding from elongation/CMG binding.

    Evidence In vitro replication and helicase/roadblock-bypass assays with purified CMG and Mcm10, mutagenesis and AID depletion in S. cerevisiae

    PMID:28270517 PMID:28869037

    Open questions at the time
    • Structural basis of the duplex-to-strand-exclusion isomerization unresolved
    • Contribution to lagging-strand engagement in vivo not fully defined
  12. 2018 High

    Defined MCM10's strand-annealing activity and its physical footprint on six CMG subunits at the fork junction, linking it to suppression of SMARCAL1-driven fork regression.

    Evidence In vitro strand-annealing and fork-regression assays with purified SMARCAL1, plus cross-linking mass spectrometry of CMG-Mcm10

    PMID:30598452

    Open questions at the time
    • High-resolution structure of CMG-Mcm10 lacking
    • In vivo relevance of fork-regression inhibition not directly tested
  13. 2021 High

    Connected MCM10 to genome- and telomere-protective functions, including BRCA2-dependent suppression of PRIMPOL repriming and prevention of telomere erosion under replication stress.

    Evidence Co-IP, siRNA and ssDNA-gap/fiber assays for BRCA2-PRIMPOL; patient fibroblasts, CRISPR/iPSC modeling, telomere FISH and MUS81 epistasis for telomere phenotypes

    PMID:33712616 PMID:34645815

    Open questions at the time
    • Direct demonstration that MCM10 disease mutations drive telomere defects in patients limited
    • Mechanism linking strand-annealing to PRIMPOL suppression not resolved
  14. 2024 Medium

    Extended MCM10 function to replisome processivity and repeat-tract stability, showing it stabilizes stalled replisomes and prevents fork stalling and instability at structured repeats.

    Evidence Single-molecule SV40 replisome reconstitution with human Mcm10; repeat-instability assays and live-cell fork-stalling imaging in S. cerevisiae

    PMID:38967018 PMID:39627228

    Open questions at the time
    • SV40 is a heterologous system
    • Repeat-instability mechanism dissected in single lab

Open questions

Synthesis pass · forward-looking unresolved questions
  • How MCM10's multiple in vitro activities (origin melting, CMG isomerization, strand annealing, pol-alpha handoff, RECQ4 cooperation) are temporally coordinated and structurally integrated at the firing origin remains unresolved.
  • No high-resolution structure of the MCM10-CMG-origin intermediate
  • Order of RECQ4 versus MCM10 loading not definitively settled
  • Mechanism coupling unwinding to CMG translocation undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 6 GO:0060090 molecular adaptor activity 4 GO:0098772 molecular function regulator activity 4 GO:0003723 RNA binding 2 GO:0044183 protein folding chaperone 2
Localization
GO:0000228 nuclear chromosome 4 GO:0005634 nucleus 3 GO:0005730 nucleolus 1
Pathway
R-HSA-69306 DNA Replication 5 R-HSA-1640170 Cell Cycle 3 R-HSA-73894 DNA Repair 3
Partners
BRCA2CDC45MCM2MCM6PCNAPOLA1RECQ4WDHD1
Complex memberships
CMG helicaseMCM2-7 double hexamer

Evidence

Reading pass · 59 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 Mcm10 (DNA43) is a nuclear protein that physically interacts with several members of the MCM2-7 family; loss of Mcm10 causes a dramatic reduction in DNA replication initiation at chromosomal origins and causes replication forks to pause during elongation through these same loci. Genetic analysis, two-dimensional DNA gel analysis, physical interaction assays in S. cerevisiae Molecular and cellular biology High 9154825
1992 DNA43 (MCM10) is an essential gene required for entry into or completion of S phase in S. cerevisiae, identified as encoding a 59.6 kDa protein required for DNA synthesis. Temperature-sensitive mutant screen, DNA synthesis monitoring in synchronous populations, complementation cloning Yeast High 1514326
2000 Mcm10 interacts physically with Mcm7 (a subunit of MCM2-7); diminished Mcm10–Mcm7 interaction inhibits replication initiation. Mcm10 mediates association of the MCM2-7 complex with replication origins, and interaction with Mcm7 is required for proper replication initiation and prompt release of origin-bound factors. Physical interaction assays, genetic epistasis (double-mutant rescue of mcm10-1/mcm7-1), chromatin association assays in S. cerevisiae Genes & development High 10783164
2002 Xenopus Mcm10 chromatin binding requires chromatin-bound Mcm2-7 and is independent of Cdk2 and Cdc7. In the absence of Mcm10, XCdc45 binding, XRPA binding, and initiation-dependent plasmid supercoiling are blocked, placing Mcm10 function after pre-RC assembly and before origin unwinding. Xenopus egg extract depletion/add-back, chromatin binding assays, plasmid supercoiling assay Molecular cell High 11864598
2000 Human Mcm10 (HsMcm10) associates with nuclease-resistant nuclear structures throughout S phase and dissociates in G2 phase. It interacts with human Orc2, Mcm2, and Mcm6 proteins. Nuclease-resistance fractionation, co-immunoprecipitation in COS-1 cells, yeast two-hybrid Nucleic acids research Medium 11095689
2003 Fission yeast Cdc23/Mcm10 functions after pre-RC formation: its inactivation does not affect Mcm2 chromatin association (pre-RC formation) but blocks Cdc45 chromatin binding, placing Mcm10 function between pre-RC formation and Cdc45 loading. Degron allele inactivation, chromatin binding assays by cytological approach in S. pombe Molecular biology of the cell High 12972571
2003 Fission yeast Cdc23p (Mcm10) interacts with both the Mcm complex (via selective binding to Mcm467 subunits) and Dfp1p (the regulatory subunit of Dfp1-Hsk1/Dbf4-Cdc7 kinase). Cdc23p is required for efficient phosphorylation of Mcm2p and Mcm4p within the six-subunit Mcm complex by Dfp1-Hsk1 kinase in vitro; this activity requires the N-terminus of Cdc23p. In vitro kinase assay with purified components, co-immunoprecipitation, truncation analysis Proceedings of the National Academy of Sciences of the United States of America High 12604790
2004 Mcm10 binding to replication origins in budding yeast is cell-cycle regulated and dependent on Mcm2-7. Mcm10 is required to maintain steady-state levels of the catalytic subunit of DNA polymerase-alpha (pol-alpha); depletion of Mcm10 during S phase causes degradation of pol-alpha without affecting Cdc45. ChIP, temperature-sensitive degron (mcm10-td) mutants, immunoblotting in S. cerevisiae Molecular cell High 15494305
2004 Mcm10 physically interacts with Cdc45 and facilitates recruitment of Cdc45 to the ARS1 origin. Overexpression of either Mcm10 or Cdc45 suppresses the growth defect of mcm10-1, and the physical Cdc45-Mcm10 interaction is disrupted in the mcm10-1 mutant. ChIP at ARS1, co-immunoprecipitation, overexpression suppression analysis in S. cerevisiae Journal of molecular biology Medium 15201046
2006 Mcm10 contains a PCNA-interacting (PIP) box and directly interacts with PCNA; only the diubiquitinated form of Mcm10 binds PCNA. Diubiquitination of Mcm10 is cell-cycle regulated (appears in late G1, persists through S phase) and associated with chromatin. A PIP-box mutation (Y245A) abolishing PCNA interaction is lethal, rescued by a compensatory PCNA mutation. PIP-box mutational analysis, in vitro pulldown, co-IP, cell-cycle fractionation, genetic rescue in S. cerevisiae Molecular and cellular biology High 16782870
2006 A conserved Hsp10-like domain in Mcm10 is required to stabilize the catalytic subunit of DNA polymerase-alpha (Cdc17/Pol1); single residue substitution in this domain dramatically reduces Cdc17 steady-state levels. Mcm10 co-overexpression stabilizes Cdc17 subject to rapid degradation, consistent with Mcm10 acting as a nuclear chaperone for Cdc17. Temperature-sensitive degron mutants, overexpression rescue, site-directed mutagenesis, immunoblotting in S. cerevisiae The Journal of biological chemistry High 16675460
2007 And-1/Ctf4 interacts with Mcm10 (which associates with MCM2-7) and with the p180 subunit of DNA pol alpha. In Xenopus egg extracts, And-1 chromatin loading requires Mcm10; antibody disrupting the Mcm10-And-1 interaction prevents loading of And-1 and pol alpha, inhibiting DNA synthesis. And-1 is essential for DNA synthesis and stability of p180. Co-immunoprecipitation, Xenopus egg extract depletion/antibody inhibition, chromatin binding assays Genes & development High 17761813
2007 Human Mcm10 interacts with and stabilizes the catalytic subunit of pol-alpha (p180) in human HeLa cells; siRNA-mediated depletion of Mcm10 causes degradation of p180 with similar kinetics, while the regulatory p68 subunit is unaffected. Simultaneous loss of Mcm10 and p180 causes S phase entry inhibition and DNA damage. siRNA knockdown, immunoblotting, cell-cycle analysis in HeLa cells Molecular biology of the cell High 17699597
2007 Human MCM10 forms a ring-shaped hexameric structure with large central and smaller lateral channels and a system of inner chambers, as determined by electron microscopy. Electron microscopy and single-particle analysis of purified human MCM10 EMBO reports Medium 17823614
2007 The highly conserved internal domain (Mcm10-ID) from Xenopus binds ssDNA via an OB-fold followed by a variant zinc finger. NMR chemical shift perturbation and mutagenesis of DNA-binding residues confirm the ssDNA-binding surface; corresponding mutations in S. cerevisiae increase sensitivity to replication stress. X-ray crystallography of Mcm10-ID, NMR chemical shift perturbation, site-directed mutagenesis, replication stress assays Structure High 19081065
2007 Xenopus Mcm10 has three structural domains: N-terminal (homodimerization), internal (ID), and C-terminal (CTD). The ID and CTD both bind ssDNA and dsDNA with low micromolar affinity and independently bind the N-terminal 323 residues of the pol alpha p180 subunit. Structural integrity of ID and CTD depends on bound zinc. Limited proteolysis, analytical ultracentrifugation, DNA binding assays, atomic absorption spectroscopy, co-IP with pol alpha fragments The Journal of biological chemistry High 18065420
2009 Human RECQ4 forms a complex with MCM10, MCM2-7 helicase, CDC45, and GINS on chromatin in a cell-cycle-regulated manner. MCM10 is essential for the integrity of the RECQ4-MCM replicative helicase complex; MCM10 interacts directly with RECQ4 and regulates its DNA unwinding activity. This interaction may be modulated by CDK phosphorylation. Purification of chromatin-bound complexes, co-immunoprecipitation, in vitro DNA unwinding assay The EMBO journal High 19696745
2009 CMG complex formation in human cells requires MCM10, RecQL4, and Ctf4/And-1 in addition to the CMG components. CMG assembly occurs only after G1/S transition and requires CDK and Cdc7 kinase activities; depletion of MCM10 by siRNA abolishes CMG complex formation. Bimolecular fluorescence complementation (BiFC) in HeLa cells, siRNA knockdown Proceedings of the National Academy of Sciences of the United States of America Medium 19805216
2009 Mcm10 ID and CTD both bind the OB-fold cleft of Mcm10-ID for ssDNA; ssDNA and pol-alpha p180 compete for binding to the OB-fold of Mcm10-ID. The minimal Mcm10-binding site on p180 maps to residues 286-310 within the p180 N-terminal domain, suggesting a handoff mechanism for pol alpha loading. X-ray crystallography of Mcm10-ID·ssDNA complex, NMR chemical shift perturbation, fluorescence spectroscopy The Journal of biological chemistry High 19608746
2009 Saccharomyces cerevisiae Mcm10 binds both dsDNA and ssDNA stably; on longer dsDNA, multiple copies cooperate to assemble a large nucleoprotein complex with ~21-24 bp spacing. On ssDNA, approximately 3 copies of Mcm10 assemble per short ssDNA oligomer, suggesting multisubunit complex formation on ssDNA. EMSA, surface plasmon resonance with purified ScMcm10 The Journal of biological chemistry Medium 19605346
2001 Human Mcm10 protein levels are regulated by cell-cycle-dependent proteolysis (proteasome-mediated degradation in late M/G1 phase) and by hyperphosphorylation in G2/M phase; Mcm10 binds chromatin at the onset of S phase and dissociates in G2/M. Cell synchronization, immunoblotting, proteasome inhibitor treatment, lambda phosphatase treatment, chromatin fractionation The Journal of biological chemistry High 11602595
2004 Human Mcm10 localizes to discrete nuclear foci during S phase; GFP-Mcm10 foci patterns (early S: throughout nucleus; mid S: nuclear periphery/nucleolus; late S: nucleoli) precede changes in replication foci by 30-60 min, suggesting Mcm10 is temporarily recruited to replication sites before they replicate and dissociates after pre-RC activation. GFP live-cell imaging in stable HeLa cell lines, pulse-labeling with BrdU to mark active replication foci The Journal of biological chemistry Medium 15136575
2010 Solution NMR structure of the Xenopus Mcm10 C-terminal domain (CTD) reveals two zinc binding motifs; only the N-terminal CCCH-type zinc motif binds ssDNA, while the CCCC-type motif is structurally similar to the zinc ribbon in MCM helicase N-terminal oligomerization domain but does not bind DNA. The ID and CTD are structurally independent in solution. Solution NMR, NMR chemical shift perturbation, mutagenesis The Journal of biological chemistry High 20489205
2012 Yeast Mcm10 interacts preferentially with the inactive Mcm2-7 double hexamer loaded at origins (not with the replisome). When Mcm10 is acutely degraded, CMG (Cdc45 and GINS) still assembles on Mcm2-7 at origins, but origin DNA unwinding is blocked — establishing a novel step after CMG assembly requiring Mcm10. Mcm10 also chaperones pol alpha in both yeast and human cells. Novel auxin-inducible degron (AID) for acute depletion, ChIP, biochemical fractionation in S. cerevisiae and human cells The EMBO journal High 22433841
2012 In fission yeast, depletion of Mcm10 to <0.5% does not affect origin loading of Mcm2-7, Cdc45, or GINS, but impairs RPA and DNA polymerase recruitment, demonstrating that Mcm10 is required for origin DNA unwinding after CMG loading. A conserved zinc finger in Mcm10 is required for RPA loading. Promoter shut-off combined with auxin-inducible degron (off-AID), ChIP, zinc finger mutagenesis in S. pombe The EMBO journal High 22433840
2012 In budding yeast, depletion of Mcm10 using auxin-inducible degron assembles a stable CMG complex at origins but prevents CMG translocation, RPA loading, and intra-S checkpoint activation. Mcm10 associates with origins during initiation in an S-cyclin-dependent kinase- and Cdc45-dependent manner. Auxin-inducible degron, ChIP, two-dimensional gel electrophoresis in S. cerevisiae Current biology High 22285032
2003 Mcm10 self-assembles into large homocomplexes (~800 kDa) mediated by a conserved 210 aa domain containing a novel zinc finger (CX10CX11CX2H) essential for homocomplex formation; cysteine/histidine mutations that abolish zinc finger cause defects in homocomplex assembly, DNA replication, and cell growth. Co-immunoprecipitation, gel filtration, zinc finger mutagenesis, cell growth assays in S. cerevisiae The Journal of biological chemistry High 12844493
2013 Mcm10 self-association is mediated by a conserved coiled-coil (CC) motif within the N-terminal domain; crystal structure at 2.4 Å reveals a three-helix bundle consistent with dimeric and trimeric assemblies. Mutations at the subunit interface disrupt in vitro dimerization and in vivo self-interaction. X-ray crystallography, analytical ultracentrifugation, yeast two-hybrid, site-directed mutagenesis PloS one High 23894664
2013 Human SIRT1 deacetylase binds and deacetylates Mcm10 both in vivo and in vitro, modulating Mcm10 stability and DNA binding ability. The two DNA-binding domains (ID and CTD) are regulated differently by acetylation/deacetylation; Mcm10 and SIRT1 act synergistically for replication fork initiation. Co-immunoprecipitation, in vitro deacetylation assay, DNA binding assays, domain-specific analysis Nucleic acids research Medium 23449222
2014 RBBP6 ubiquitin ligase ubiquitinates and destabilizes the transcriptional repressor ZBTB38, which negatively regulates MCM10 levels on chromatin. Cells lacking RBBP6 accumulate ZBTB38, reduce MCM10 chromatin levels, experience reduced replication fork progression and increased damage at common fragile sites. siRNA knockdown, ubiquitination assays, replication fork progression analysis, CFS analysis Cell reports Medium 24726359
2015 HIV-1 Vpr indirectly binds MCM10 in a VprBP-dependent manner and enhances ubiquitination and proteasomal degradation of MCM10 via the Cul4-DDB1[VprBP] E3 ubiquitin ligase. G2/M-defective Vpr mutants cannot deplete MCM10, linking MCM10 degradation to Vpr-induced G2/M arrest. Co-immunoprecipitation, ubiquitination assay, proteasome inhibitor treatment, Vpr mutant analysis The Journal of biological chemistry Medium 26032416
2012 CRL4-DDB1-VprBP ubiquitin ligase mediates UV-stress-induced proteolysis of Mcm10. Depletion of DDB1, Roc1, or Cul4 abrogates UV-triggered Mcm10 proteolysis. Purified Cul4-Roc1-DDB1 complex ubiquitinates Mcm10 in vitro; VprBP is the substrate recognition subunit targeting Mcm10. siRNA knockdown, in vitro ubiquitination assay with purified components, immunoblotting Nucleic acids research High 22570418
2015 Mcm10 is recruited to replication initiation sites via direct binding to MCM through its C-terminus. The Mcm10 binding site on MCM includes Mcm2 and Mcm6 subunits and overlaps with the Cdt1-binding site. Mcm10 exhibits low-affinity recruitment in absence of CMG assembly and high-affinity recruitment when CMG assembles; Mcm10 unable to bind MCM directly cannot support DNA replication. Pulldown with purified proteins, biochemical fractionation, replication reconstitution assay in budding yeast The Journal of biological chemistry High 26719337
2015 Mcm10 co-purifies exclusively with endogenous MCM2-7 double hexamers (DHs) on chromatin in G1 phase. Deletion of the Mcm10 C-terminus (main interaction domain with MCM) causes growth and S-phase defects; Mcm10-MCM fusion restores function. Mcm10 interaction-deficient mutants show delayed DH dissolution during S phase, suggesting Mcm10 promotes Mcm2-7 remodeling. Cellular fractionation, endogenous DH purification, chromatin immunoprecipitation, Mcm10-MCM fusion genetic rescue in S. cerevisiae Cell reports High 26686640
2015 Mcm10 directly interacts with the Mcm2-7 complex and Cdc45, recruits Cdc45 to Mcm2-7 in vitro, stimulates Mcm2 phosphorylation by DDK in vivo and in vitro, and promotes timely recruitment of Cdc45 and GINS to Mcm2-7 during early S phase. In vitro pulldown with purified proteins, auxin-inducible degron, in vitro DDK phosphorylation assay, ChIP Nucleic acids research High 26582917
2014 The N-terminus of Mcm10 interacts with the Mec3 subunit of the 9-1-1 clamp in response to replication stress (UV or HU). Truncation of the Mcm10 N-terminus causes UV sensitivity; this is not enhanced by MEC3 deletion, placing Mcm10 and Mec3 in the same pathway for resistance to replication stress. Co-immunoprecipitation, truncation analysis, genetic epistasis (double-mutant analysis), UV sensitivity assays in S. cerevisiae Nucleic acids research Medium 24972833
2017 Mcm10 binds a conserved motif between the OB-fold and A subdomain of Mcm2; mutations predicted to expose this motif restore growth to conditional-lethal MCM10 mutants. Mcm10 stabilizes Cdc45 and GINS association with Mcm2-7, stimulates replication elongation in vivo and in vitro, and a lethal allele that stimulates initial unwinding but is defective in elongation and CMG binding was identified. In vitro replication assay, CMG binding assay with purified proteins, mutagenesis, AID-based depletion in S. cerevisiae Genes & development High 28270517
2017 Mcm10 binds CMG and greatly stimulates CMG helicase activity in vitro. Mcm10 enables CMG and the replisome to bypass blocks on the non-tracking (lagging) strand without displacing the blocks, indicating Mcm10 isomerizes the CMG-DNA complex from a duplex-encircling to a strand-exclusion mode. In vitro helicase/translocation assays with purified CMG and Mcm10, roadblock bypass assays eLife High 28869037
2018 Mcm10 has potent strand-annealing activity both alone and in complex with CMG; CMG-Mcm10 unwinds and reanneals single strands in vitro. Mcm10 inhibits fork regression by the SMARCAL1 fork reversal enzyme. Cross-linking mass spectrometry shows Mcm10 contacts six CMG subunits, with the Mcm10 DNA-binding region on the N-face of CMG, placing it at the fork junction. In vitro strand annealing assay, fork regression assay with purified SMARCAL1, cross-linking mass spectrometry of CMG-Mcm10 complex Proceedings of the National Academy of Sciences of the United States of America High 30598452
2016 In Xenopus egg extracts, depletion of >99% of Mcm10 does not prevent the bulk of DNA replication but reduces fork elongation rate; absence of Mcm10 or its CDK phosphorylation results in instability of replisome proteins on DNA, particularly under replication stress. Mcm10 is a CDK substrate but does not require phosphorylation for chromatin association. Xenopus egg extract depletion (>99%), DNA fiber analysis, replisome stability assay, phospho-mutant analysis Cell cycle Medium 27327991
2013 Human Mcm10 and Cdc45 interact directly: the Mcm10 CTD binds Cdc45 by co-immunoprecipitation and surface plasmon resonance; the ID interacts with Cdc45 only in presence of DNA. Both ID and CTD bind bubble and fork DNA structures preferentially and enhance Cdc45 DNA-binding affinity. Co-immunoprecipitation from cell-free extracts, surface plasmon resonance, domain truncation analysis, DNA binding assays with human proteins The Biochemical journal Medium 23750504
2015 RecQL4 is required for the origin binding of Mcm10 and Ctf4 in human cells; their association with replication origins requires both CDK and DDK activities and the presence of the pre-replicative complex. Physical interactions and origin association are targeted by DNA damage checkpoint pathways. ChIP, siRNA knockdown, cell-cycle synchronization, checkpoint inhibition in human cells Cell cycle Medium 25602958
2017 Human MCM10 interaction with Mcm2-7 requires the domain containing amino acids 530-655, which overlaps with the domain required for stable chromatin retention. The conserved domain (aa 200-482) is essential for DNA replication; Mcm10 depletion reduces replication initiation frequency and impairs RPA, pol alpha, and PCNA chromatin loading without affecting Cdc45 and pol epsilon loading. siRNA + complementation with truncation constructs, chromatin fractionation, DNA fiber analysis in human HeLa cells The Journal of biological chemistry Medium 28646110
2021 BRCA2 associates with MCM10 and this association suppresses PRIMPOL-mediated repriming and ssDNA gap formation after DNA damage, while having no impact on stability of stalled replication forks. Co-immunoprecipitation, siRNA knockdown, ssDNA gap detection assays, fiber assays Nature communications Medium 34645815
2021 MCM10 deficiency causes chronic replication stress, genomic instability, and telomere erosion. Loss of MCM10 function constrains telomerase activity by accumulating abnormal replication fork structures enriched with ssDNA. Terminally-arrested forks in MCM10-deficient cells require endonucleolytic processing by MUS81; MCM10:MUS81 double mutants display decreased viability and accelerated telomere shortening. Patient fibroblast analysis, CRISPR modeling, MCM10 knockdown in NK cell lines, iPSC-derived NK cells, telomere FISH, DNA fiber assays Nature communications High 33712616
2023 During DNA replication termination under topological stress in vertebrates, RTEL1 and MCM10 are highly enriched on chromatin during fork convergence and cooperate to promote fork convergence; they do not impact topoisomerase activity but promote fork progression through replication barriers. Proteomics in Xenopus egg extracts, chromatin enrichment, functional depletion assays Cell reports Medium 36807139
2016 An Mcm10 mutant (Mcm10-m2,3,4) defective in ssDNA binding in vitro also fails to stimulate DDK phosphorylation of Mcm2 in vitro. Expression in yeast causes severe growth and replication defects, reduced DDK-phosphorylated Mcm2 and GINS association with Mcm2-7 in vivo. Defects in origin melting persist in the mcm5-bob1 bypass background, indicating ssDNA binding by Mcm10 is independently required for origin melting. In vitro DNA binding and DDK phosphorylation assays, genetic analysis with mcm5-bob1, ChIP in S. cerevisiae Journal of molecular biology High 27751725
2017 An intact Mcm10 coiled-coil (NTD) interaction surface is required for stimulating DDK phosphorylation of Mcm2, binding long ssDNA, and recruiting pol alpha to Mcm2-7 in vitro. Mcm10-4A NTD mutant causes severe replication defects and diminished origin melting and GINS recruitment even in the mcm5-bob1 bypass background. In vitro phosphorylation and pol alpha recruitment assays, mcm5-bob1 genetic epistasis, ChIP, growth assays in S. cerevisiae Nucleic acids research High 28510759
2019 Suramin and several analogues directly inhibit Mcm10 DNA binding in vitro (competition fluorescence polarization, surface plasmon resonance) and reduce replication products in an in vitro replication assay. Binding is confirmed by SPR and selectivity for Mcm10 over human RPA demonstrated for some analogues. Fluorescence polarization HTS, surface plasmon resonance, in vitro replication assay Open biology Medium 31409229
2024 Deficiency of Mcm10 dramatically elevates (GAA)n repeat instability in yeast; live-cell microscopy shows increased replication fork stalling at the repeat in mcm10-1 cells. Viability of strains with (GAA)100 repeat at an essential chromosomal location strongly depends on Mcm10 function. Rad9 checkpoint activation promotes viability but initiates repeat expansions via pol delta; when RPA is depleted, breakage of under-replicated repetitive DNA occurs in G2/M. Repeat instability assays, live-cell microscopy of replication fork stalling, genetic analysis, RPA depletion in S. cerevisiae Nature communications Medium 39627228
2016 S. cerevisiae Mcm10 ubiquitination maps primarily to lysine 372; mutation of K372 to arginine ablates ubiquitination of overexpressed protein and causes hydroxyurea sensitivity in S-phase checkpoint-compromised cells, linking Mcm10 ubiquitination to suppression of replication stress. Mass spectrometry mapping, site-directed mutagenesis, hydroxyurea sensitivity assay in S. cerevisiae Biochemistry and biophysics reports Medium 28497125
2021 A germline variant rs2274110 in MCM10 exon 15 increases SUMOylation levels at K669 of MCM10 protein mediated by SUMO2/3 enzymes, leading to aberrant MCM10 overexpression and increased genomic instability (DNA over-replication). Exome-wide association study followed by functional SUMOylation assays, site-directed mutagenesis of K669, cell proliferation and genome instability assays Clinical and translational medicine Medium 34185429
2025 Using single-molecule imaging and ensemble biochemistry, Mcm10 and RecQL4 act in a concerted manner to activate the replicative CMG helicase: Mcm10 binds first to inactive helicases and recruits RecQL4, which synergizes with Mcm10 to promote helicase activation. Mcm10 is not incorporated into replisomes and dissociates from origins during replication initiation. In the absence of Mcm10, RecQL4 is recruited to origins via interaction with the Mcm7 subunit. Single-molecule imaging, ensemble biochemistry with purified proteins, genetic interaction analysis bioRxivpreprint Medium 40799587
2024 In an in vitro SV40 replisome reconstitution, Mcm10 increases processivity of the replisome by stabilizing stalled replisomes and increasing their chances of restarting synthesis without altering the rate. Single-molecule in vitro reconstitution of SV40 replisome with purified human Mcm10 Nucleic acids research Medium 38967018
2003 Drosophila Mcm10 interacts with key members of the prereplication complex: Mcm2, Dup (Cdt1), and Orc2, as well as with Cdc45 and HP1. RNAi depletion of Mcm10 in KC cells results in loss of DNA content and aberrant chromosome condensation. Yeast two-hybrid, RNAi depletion in Drosophila KC cells, DNA content analysis Molecular biology of the cell Medium 12808023
2003 Fission yeast Cdc23/Mcm10 is bound to chromatin throughout the cell cycle in growing cells and only displaced during quiescence; re-establishment of chromatin binding upon return to growth is independent of pre-RC formation. Chromatin fractionation assays throughout cell cycle in S. pombe Molecular biology of the cell Medium 12972571
2008 Mcm10 C-terminal domain mediates interaction with Sir2; mutations in the C-terminal 108 aa of Mcm10 destroy two-hybrid interactions with Sir2 and Sir3. Mcm10 mediates the interaction between replication proteins (Mcm3, Mcm7) and the silencing factor Sir2 via its C-terminal domain, contributing to heterochromatic silencing independently of DNA replication. Yeast two-hybrid, silencing reporter assays, genetic analysis in S. cerevisiae Genetics Medium 19064704
2019 Fission yeast Cdc23/Mcm10 mutations in the pol-alpha-recruitment and putative primase homology domain abrogate ribonucleotide imprint formation at the mat1 locus, suggesting Mcm10 plays a direct role in installing the ribonucleotide imprint in cooperation with pol alpha and Swi1. Genetic analysis of mcm10/cdc23 domain mutants, alkaline-labile imprint assay in S. pombe Nucleic acids research Medium 30759238
2003 Two bipartite NLSs in Mcm10 mediate its constitutive nuclear localization; either NLS alone is sufficient to direct Mcm10 (and GFP fused to it) into the nucleus. NLS mutation analysis, GFP fusion localization in S. cerevisiae Current genetics Medium 13680157

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication. Genes & development 180 17761813
2004 Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha. Molecular cell 172 15494305
2009 Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proceedings of the National Academy of Sciences of the United States of America 162 19805216
2002 Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45. Molecular cell 158 11864598
2000 Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins. Genes & development 142 10783164
1997 A lesion in the DNA replication initiation factor Mcm10 induces pausing of elongation forks through chromosomal replication origins in Saccharomyces cerevisiae. Molecular and cellular biology 124 9154825
2009 MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication. The EMBO journal 119 19696745
2012 Mcm10 associates with the loaded DNA helicase at replication origins and defines a novel step in its activation. The EMBO journal 112 22433841
2017 Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase. Genes & development 104 28270517
2012 Mcm10 plays a role in functioning of the eukaryotic replicative DNA helicase, Cdc45-Mcm-GINS. Current biology : CB 93 22285032
2012 Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components. The EMBO journal 92 22433840
2003 Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding. Molecular biology of the cell 89 12972571
2017 Mcm10 promotes rapid isomerization of CMG-DNA for replisome bypass of lagging strand DNA blocks. eLife 81 28869037
2000 The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase. Nucleic acids research 74 11095689
2003 The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase. Proceedings of the National Academy of Sciences of the United States of America 73 12604790
2007 Human Mcm10 regulates the catalytic subunit of DNA polymerase-alpha and prevents DNA damage during replication. Molecular biology of the cell 72 17699597
2021 BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage. Nature communications 71 34645815
2006 Interaction between PCNA and diubiquitinated Mcm10 is essential for cell growth in budding yeast. Molecular and cellular biology 70 16782870
2003 Drosophila MCM10 interacts with members of the prereplication complex and is required for proper chromosome condensation. Molecular biology of the cell 68 12808023
2004 Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae. Journal of molecular biology 66 15201046
2017 Mcm10: A Dynamic Scaffold at Eukaryotic Replication Forks. Genes 65 28218679
2013 Enigmatic roles of Mcm10 in DNA replication. Trends in biochemical sciences 64 23332289
1992 Genetic and molecular analysis of DNA43 and DNA52: two new cell-cycle genes in Saccharomyces cerevisiae. Yeast (Chichester, England) 62 1514326
2020 Human NK cell deficiency as a result of biallelic mutations in MCM10. The Journal of clinical investigation 61 32865517
2014 The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell reports 61 24726359
2001 Cell cycle-dependent proteolysis and phosphorylation of human Mcm10. The Journal of biological chemistry 56 11602595
2015 Cell-Cycle-Regulated Interaction between Mcm10 and Double Hexameric Mcm2-7 Is Required for Helicase Splitting and Activation during S Phase. Cell reports 53 26686640
2008 Structural basis for DNA binding by replication initiator Mcm10. Structure (London, England : 1993) 52 19081065
2015 Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex. The Journal of biological chemistry 49 26719337
2015 HIV-1 Vpr Protein Enhances Proteasomal Degradation of MCM10 DNA Replication Factor through the Cul4-DDB1[VprBP] E3 Ubiquitin Ligase to Induce G2/M Cell Cycle Arrest. The Journal of biological chemistry 48 26032416
2014 MCM10: one tool for all-Integrity, maintenance and damage control. Seminars in cell & developmental biology 48 24662891
2009 Physical interactions between Mcm10, DNA, and DNA polymerase alpha. The Journal of biological chemistry 48 19608746
2007 Domain architecture and biochemical characterization of vertebrate Mcm10. The Journal of biological chemistry 48 18065420
2006 A conserved Hsp10-like domain in Mcm10 is required to stabilize the catalytic subunit of DNA polymerase-alpha in budding yeast. The Journal of biological chemistry 47 16675460
2004 Expression of MCM10 and TopBP1 is regulated by cell proliferation and UV irradiation via the E2F transcription factor. Oncogene 45 15195143
2001 Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints. Genetics 42 11606526
2012 CRL4-DDB1-VPRBP ubiquitin ligase mediates the stress triggered proteolysis of Mcm10. Nucleic acids research 41 22570418
2018 Mcm10 has potent strand-annealing activity and limits translocase-mediated fork regression. Proceedings of the National Academy of Sciences of the United States of America 39 30598452
2010 Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM. Cell cycle (Georgetown, Tex.) 39 20237432
2003 Budding yeast mcm10/dna43 mutant requires a novel repair pathway for viability. Genes to cells : devoted to molecular & cellular mechanisms 37 12694535
2005 Nuclear distribution and chromatin association of DNA polymerase alpha-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast. BMC molecular biology 36 15941470
2003 A novel zinc finger is required for Mcm10 homocomplex assembly. The Journal of biological chemistry 35 12844493
2007 Hexameric ring structure of human MCM10 DNA replication factor. EMBO reports 33 17823614
2015 RecQL4 is required for the association of Mcm10 and Ctf4 with replication origins in human cells. Cell cycle (Georgetown, Tex.) 32 25602958
1998 The essential schizosaccharomyces pombe cdc23 DNA replication gene shares structural and functional homology with the Saccharomyces cerevisiae DNA43 (MCM10) gene. Current genetics 32 9745018
2021 Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shortening. Nature communications 31 33712616
2021 MCM10 compensates for Myc-induced DNA replication stress in breast cancer stem-like cells. Cancer science 30 33340428
2015 Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells. Oncotarget 29 26588054
2009 Novel DNA binding properties of the Mcm10 protein from Saccharomyces cerevisiae. The Journal of biological chemistry 29 19605346
2013 Human SIRT1 regulates DNA binding and stability of the Mcm10 DNA replication factor via deacetylation. Nucleic acids research 27 23449222
2015 Mcm10 coordinates the timely assembly and activation of the replication fork helicase. Nucleic acids research 26 26582917
2014 The N-terminus of Mcm10 is important for interaction with the 9-1-1 clamp and in resistance to DNA damage. Nucleic acids research 26 24972833
2016 Xenopus Mcm10 is a CDK-substrate required for replication fork stability. Cell cycle (Georgetown, Tex.) 25 27327991
2014 Mcm10 deficiency causes defective-replisome-induced mutagenesis and a dependency on error-free postreplicative repair. Cell cycle (Georgetown, Tex.) 25 24674891
2004 Localization of human Mcm10 is spatially and temporally regulated during the S phase. The Journal of biological chemistry 25 15136575
2010 Multiple functions for Drosophila Mcm10 suggested through analysis of two Mcm10 mutant alleles. Genetics 24 20498296
2008 Mcm10 mediates the interaction between DNA replication and silencing machineries. Genetics 23 19064704
2023 RTEL1 and MCM10 overcome topological stress during vertebrate replication termination. Cell reports 22 36807139
2011 Targeted disruption of Mcm10 causes defective embryonic cell proliferation and early embryo lethality. Biochimica et biophysica acta 22 21693137
2010 Ultraviolet radiation stress triggers the down-regulation of essential replication factor Mcm10. The Journal of biological chemistry 22 20064936
2007 Knockdown of human MCM10 exhibits delayed and incomplete chromosome replication. Biochemical and biophysical research communications 22 17997977
2005 Mcm10 is required for the maintenance of transcriptional silencing in Saccharomyces cerevisiae. Genetics 21 16085704
2011 Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1). DNA repair 20 21945095
2007 Knockdown of human MCM10 activates G2 checkpoint pathway. Biochemical and biophysical research communications 20 17997981
2021 Aberrant MCM10 SUMOylation induces genomic instability mediated by a genetic variant associated with survival of esophageal squamous cell carcinoma. Clinical and translational medicine 19 34185429
2012 Structural biology of replication initiation factor Mcm10. Sub-cellular biochemistry 19 22918587
2010 Solution NMR structure of the C-terminal DNA binding domain of Mcm10 reveals a conserved MCM motif. The Journal of biological chemistry 19 20489205
2017 The Mcm2-7-interacting domain of human mini-chromosome maintenance 10 (Mcm10) protein is important for stable chromatin association and origin firing. The Journal of biological chemistry 18 28646110
2013 The physical interaction of Mcm10 with Cdc45 modulates their DNA-binding properties. The Biochemical journal 18 23750504
2003 Two bipartite NLSs mediate constitutive nuclear localization of Mcm10. Current genetics 17 13680157
2013 Mcm10 self-association is mediated by an N-terminal coiled-coil domain. PloS one 16 23894664
2005 Dual roles for Mcm10 in DNA replication initiation and silencing at the mating-type loci. Molecular biology reports 14 16328881
2019 The anti-parasitic agent suramin and several of its analogues are inhibitors of the DNA binding protein Mcm10. Open biology 13 31409229
2016 An Mcm10 Mutant Defective in ssDNA Binding Shows Defects in DNA Replication Initiation. Journal of molecular biology 13 27751725
2006 The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast. Cell division 13 17112379
2024 Multifaceted role of the DNA replication protein MCM10 in maintaining genome stability and its implication in human diseases. Cancer metastasis reviews 10 39240414
2022 Comprehensive analysis of DNA replication timing across 184 cell lines suggests a role for MCM10 in replication timing regulation. Human molecular genetics 10 35394024
2020 Knockdown of MCM10 Gene Impairs Glioblastoma Cell Proliferation, Migration and Invasion and the Implications for the Regulation of Tumorigenesis. Journal of molecular neuroscience : MN 10 32030558
2019 Fine-tuning of the replisome: Mcm10 regulates fork progression and regression. Cell cycle (Georgetown, Tex.) 10 31014174
2025 The m6A reader IGF2BP3 promotes HCC progression by enhancing MCM10 stability. Scientific reports 9 40065065
2024 Stabilization of expandable DNA repeats by the replication factor Mcm10 promotes cell viability. Nature communications 9 39627228
2017 An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-α to Mcm2-7. Nucleic acids research 9 28510759
2024 A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells. Open biology 8 38262603
2025 O-GlcNAcylation of METTL3 drives hepatocellular carcinoma progression by upregulating MCM10 expression in an m6A-IGF2BP3-dependent manner. Cell death & disease 7 40651967
2019 Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast. Nucleic acids research 7 30759238
2023 Activation of Glycolysis by MCM10 Increases Stemness and Paclitaxel Resistance in Gastric Cancer Cells. The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 6 37860833
2021 High expression of MCM10 is predictive of poor outcomes in lung adenocarcinoma. PeerJ 6 33604163
2020 Distinct MCM10 Proteasomal Degradation Profiles by Primate Lentiviruses Vpr Proteins. Viruses 6 31952107
2017 Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic acids research 6 28180289
2015 Mcm10 is required for oogenesis and early embryogenesis in Drosophila. Mechanisms of development 6 26369283
2023 Minichromosome maintenance protein 10 (mcm10) regulates hematopoietic stem cell emergence in the zebrafish embryo. Stem cell reports 5 37437546
2023 MCM10 expression is linked to cervical cancer aggressiveness. Frontiers in molecular medicine 5 39086679
2014 Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye. PloS one 5 24686397
2010 Mcm10 proteolysis initiates before the onset of M-phase. BMC cell biology 5 20979666
2024 Investigation into in silico and in vitro approaches for inhibitors targeting MCM10 in Leishmania donovani: a comprehensive study. Molecular diversity 4 38722455
2025 Targeting MCM10 disrupts cancer stemness and counteracts sorafenib resistance in hepatocellular carcinoma. Cancer gene therapy 3 40750706
2018 Ablation of MCM10 using CRISPR/Cas9 restrains the growth and migration of esophageal squamous cell carcinoma cells through inhibition of Akt signaling. OncoTargets and therapy 3 29922071
2016 Mapping ubiquitination sites of S. cerevisiae Mcm10. Biochemistry and biophysics reports 3 28497125
2025 Mcm10 and RecQL4 Synergize to Activate the Eukaryotic Replicative DNA Helicase. bioRxiv : the preprint server for biology 2 40799587
2024 Single-molecule characterization of SV40 replisome and novel factors: human FPC and Mcm10. Nucleic acids research 2 38967018

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