Affinage

MCM6

DNA replication licensing factor MCM6 · UniProt Q14566

Length
821 aa
Mass
92.9 kDa
Annotated
2026-04-28
56 papers in source corpus 22 papers cited in narrative 22 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MCM6 is an essential subunit of the MCM2-7 replicative helicase that couples DNA replication licensing, fork progression, and checkpoint signaling. Its ATPase activity within the MCM4-MCM6-MCM7 sub-complex is required for DNA unwinding, with Walker B and arginine finger motifs at the Mcm6/2 interface contributing to ring gating (PMID:10567526, PMID:20484375); its C-terminal winged-helix Cdt1-binding domain directly recruits Cdt1 to load the hexamer onto replication origins during G1, a step blocked by geminin (PMID:12192004, PMID:20623209). MCM6 also serves as a signaling platform: its C-terminus binds the checkpoint mediator Mrc1 to activate a DNA-damage-specific replication checkpoint, and its N- and C-terminal domains interact with BLM helicase in a cell-cycle-dependent manner to negatively regulate replication speed (PMID:19620285, PMID:34370039). MCM6 transcription is induced by E2F, YAP-TEAD, and c-Myc, and is repressed by LINC00472-directed promoter methylation; the protein is regulated post-translationally by CDK-mediated phosphorylation, SIRT7-dependent crotonylation at K599, and ubiquitination by RNF125 (PMID:10327050, PMID:36185598, PMID:39805445, PMID:17005684, PMID:39477811, PMID:38298426).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1997 Medium

    Establishing that MCM6 exists in cell-cycle-regulated chromatin-bound and soluble pools as part of an MCM heteromeric complex resolved how MCM proteins partition during the cell cycle.

    Evidence Co-immunoprecipitation and subcellular fractionation of synchronized human cells

    PMID:9286856

    Open questions at the time
    • Single lab; mechanism of chromatin release not addressed
    • Identity of all complex partners not resolved
  2. 1999 High

    Demonstrating that MCM6's ATPase motifs are specifically required for helicase activity—while MCM4 contributes ssDNA binding—separated enzymatic contributions within the MCM4/6/7 sub-complex and established MCM6 as the catalytically essential ATPase for DNA unwinding.

    Evidence In vitro helicase assays with ATPase motif mutagenesis of recombinant mouse MCM4/6/7

    PMID:10567526

    Open questions at the time
    • Assayed sub-complex, not full MCM2-7 hexamer
    • Contribution of other MCM subunit ATPase sites not tested
  3. 1999 High

    Showing that E2F transcription factor sites in the MCM6 promoter are required for serum-stimulated induction linked MCM6 expression to mitogen-driven cell cycle entry.

    Evidence Promoter-reporter mutagenesis and forced E2F1 expression in REF52 cells

    PMID:10327050

    Open questions at the time
    • Endogenous E2F ChIP not performed
    • Regulation in non-fibroblast contexts not tested
  4. 1999 High

    ChIP localization of fission yeast Mcm6 to replication origins specifically during G1/S established that MCM proteins are loaded at defined origin sequences prior to replication firing.

    Evidence ChIP and immunostaining of spread nuclei in cell-cycle-fractionated S. pombe

    PMID:10490657

    Open questions at the time
    • Mechanism of origin-specific loading not addressed
    • Fission yeast ortholog; human origin loading not directly shown
  5. 2002 High

    Mapping the direct Cdt1–MCM6 interaction to the C-terminal region of Cdt1, and showing geminin blocks this binding, established the molecular mechanism by which the MCM2-7 complex is recruited to origins and how licensing is inhibited.

    Evidence Yeast two-hybrid, recombinant protein binding, and domain-deletion mapping with geminin inhibition

    PMID:12192004

    Open questions at the time
    • Structural basis of the Cdt1-MCM6 interface not resolved at atomic level
    • Whether geminin competes or allosterically inhibits not fully distinguished
  6. 2006 High

    Showing that CDK2/cyclin A phosphorylation of MCM4 and EBV-PK phosphorylation of MCM6 itself both inactivate MCM4/6/7 helicase activity revealed how kinase signaling shuts down the replication machinery.

    Evidence In vitro helicase assays with site-directed MCM4 mutagenesis and kinase assays

    PMID:17005684

    Open questions at the time
    • Specific MCM6 phosphorylation sites not mapped
    • In vivo relevance of EBV-PK phosphorylation of MCM6 not tested
  7. 2009 High

    Identifying that MCM6's C-terminal 168-amino-acid region directly binds checkpoint mediator Mrc1, and that disruption specifically abolishes MMS- but not HU-induced checkpoint activation, established MCM6 as a damage-type-specific checkpoint sensor.

    Evidence Separation-of-function mutants, genetic suppression by Mcm6-Mrc1 fusion in S. cerevisiae

    PMID:19620285

    Open questions at the time
    • Mammalian Claspin–MCM6 interaction not directly validated
    • Mechanism discriminating MMS from HU signal unclear
  8. 2010 High

    NMR characterization of the MCM6 Cdt1-binding domain as a winged-helix fold, and Walker B/arginine finger mutagenesis at the Mcm6/2 interface affecting ring gating, provided structural understanding of both the licensing and helicase functions of MCM6.

    Evidence Triple-resonance NMR (human MCM6 CBD); systematic ATPase mutagenesis in S. cerevisiae Mcm2-7

    PMID:20484375 PMID:20623209

    Open questions at the time
    • No high-resolution co-crystal of MCM6 CBD–Cdt1 complex
    • Contribution of Mcm6/2 site to in vivo replication dynamics not measured
  9. 2010 Medium

    Quantitative flow cytometry showing two discrete chromatin-bound MCM6 states in G1—with high loading marking restriction-point passage—linked MCM loading stoichiometry to cell cycle commitment decisions.

    Evidence Multivariate flow cytometry with serum starvation/release in multiple cell lines

    PMID:20398392

    Open questions at the time
    • Causal relationship between high MCM6 loading and commitment not demonstrated by perturbation
    • Mechanism generating the switch from low to high loading unknown
  10. 2021 High

    Demonstrating that BLM helicase physically binds MCM6 in a cell-cycle-dependent manner—N-terminal domain in G1, C-terminal CBD in S-phase—and that disruption increases replication speed and delays DSB repair, established MCM6 as a platform coupling fork speed control to genome maintenance.

    Evidence Reciprocal co-IP, domain mapping, replication speed and DNA damage assays

    PMID:34370039

    Open questions at the time
    • Whether BLM competes with Cdt1 for the CBD in S-phase not tested
    • Structural basis of BLM–MCM6 interaction unknown
  11. 2021 Medium

    Showing that LINC00472 recruits DNA methyltransferases to the MCM6 promoter to silence its expression, and that MCM6 restoration reverses tumor suppression, revealed an epigenetic axis controlling MCM6 levels in breast cancer.

    Evidence LINC00472 overexpression, methylation assay at MCM6 promoter, MCM6 rescue in TNBC models

    PMID:33668040

    Open questions at the time
    • Specific DNMT identity recruited not fully resolved
    • Applicability beyond TNBC not tested
  12. 2022 High

    Identifying YAP-TEAD as a direct transcriptional activator of MCM6, and showing MCM6 feeds back via PI3K/Akt/GSK3β signaling while its loss impairs ATR/Chk1 checkpoint activation, positioned MCM6 at the intersection of Hippo signaling and replication stress response in gastric cancer.

    Evidence ChIP-PCR for TEAD at MCM6 promoter, epistasis with PI3K/Akt, xenograft and organoid models

    PMID:36185598

    Open questions at the time
    • Direct mechanism linking MCM6 to PI3K/Akt activation not identified
    • Generality of YAP-MCM6 axis across tissue types unclear
  13. 2023 Medium

    Identification of a de novo MCM6 p.(Cys158Tyr) variant disrupting the zinc-finger domain in two patients with ciliogenesis and proliferation defects in fibroblasts linked MCM6 dysfunction to a developmental phenotype, suggesting MCM6 as a candidate disease gene.

    Evidence Trio exome/genome sequencing with patient fibroblast functional assays

    PMID:37198333

    Open questions at the time
    • Only two patients identified; no animal model phenocopy
    • How MCM6 zinc-finger disruption specifically impairs ciliogenesis is unknown
  14. 2024 Medium

    Discovery that SIRT7-mediated crotonylation at MCM6-K599 increases upon replication stress due to MCM2-7 complex disassembly, with regulation by RNF8-dependent ubiquitination, revealed a novel post-translational modification axis on MCM6 responsive to fork stalling.

    Evidence Mass spectrometry PTM identification, SIRT7/RNF8 manipulation, ubiquitination assays

    PMID:39477811

    Open questions at the time
    • Functional consequence of K599 crotonylation on helicase activity or checkpoint not determined
    • Single lab; awaits independent confirmation
  15. 2024 Medium

    Showing that RNF125 ubiquitinates MCM6 to regulate its stability, and that RNF125's pro-proliferative effect in HCC is largely MCM6-dependent, identified a specific E3 ligase governing MCM6 protein homeostasis.

    Evidence Co-IP, ubiquitination assay, rescue experiments in HCC cell lines

    PMID:38298426

    Open questions at the time
    • Whether RNF125-mediated ubiquitination leads to degradation or non-degradative signaling not fully resolved
    • Specific ubiquitination sites on MCM6 not mapped
  16. 2025 Medium

    Demonstrating a c-Myc–MCM6 positive feedback loop—where c-Myc transcribes MCM6 and MCM6 stabilizes nuclear c-Myc by blocking its ubiquitin-mediated degradation—explained a mechanism of cisplatin resistance through enhanced DNA damage repair.

    Evidence c-Myc ChIP at MCM6 promoter, MCM6 knockdown affecting c-Myc ubiquitination, cisplatin sensitivity assays in vitro and in vivo

    PMID:39805445

    Open questions at the time
    • Mechanism by which MCM6 prevents c-Myc ubiquitination is unknown
    • Single lab; generality beyond the cancer type tested unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How MCM6's multiple interaction surfaces (Cdt1-binding CBD, Mrc1/Claspin-binding C-terminus, BLM-binding domains) are coordinately regulated during the cell cycle, and whether crotonylation and ubiquitination at specific sites directly modulate helicase or checkpoint activity, remain unresolved.
  • No high-resolution structure of full-length human MCM6 in complex with its regulatory partners
  • Interplay between distinct post-translational modifications on MCM6 function not systematically tested
  • Claspin (mammalian Mrc1 ortholog)–MCM6 direct binding not validated in human cells

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 3 GO:0140657 ATP-dependent activity 2
Localization
GO:0005694 chromosome 3 GO:0005634 nucleus 2
Pathway
R-HSA-69306 DNA Replication 6 R-HSA-73894 DNA Repair 3
Partners
BLMCDT1MCM2MCM4MCM7MRC1RNF125UBE3A
Complex memberships
MCM2-7 hexamerMCM4-MCM6-MCM7 sub-complex

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1999 The ATP binding activity of MCM6 within the MCM4-MCM6-MCM7 complex is critical for DNA helicase activity; mutating conserved ATPase motifs of MCM6 abolishes helicase activity while MCM4 plays a role in single-stranded DNA binding. The helicase and ssDNA binding activities of the complex can be separated. In vitro helicase assay with ATPase motif mutagenesis of recombinant mouse MCM4/6/7 expressed in insect cells Molecular and cellular biology High 10567526
1999 MCM5 and MCM6 gene expression is regulated by the transcription factor E2F through multiple E2F binding sites in their promoters; E2F sites in the MCM6 promoter (including a novel E2F-like sequence) are required for serum-stimulated transcriptional induction. Promoter-reporter assays with E2F site mutations, serum stimulation, and forced E2F1 expression in REF52 cells Oncogene High 10327050
2002 Mouse Cdt1 directly interacts with MCM6 via the C-terminal region of Cdt1 (amino acids 407–477); geminin associates with the central region of Cdt1 (amino acids 177–380) and inhibits both Cdt1-MCM6 interaction and Cdt1 DNA binding activity. Yeast two-hybrid analysis, bacterial expression and purification, domain mapping The Journal of biological chemistry High 12192004
1999 Fission yeast Mcm6 (SpMcm6p/Mis5) associates with chromosomal replication origins specifically during G1 and S phases, colocalizing with ORC at a ~1 kb region of ars2004 and ars3002, indicating it is loaded onto origins to initiate replication. Chromatin immunoprecipitation, immunostaining of spread nuclei, cell-cycle fractionation Molecular and cellular biology High 10490657
1997 Human MCM6 protein undergoes cell-cycle-dependent subcellular redistribution; it exists in two forms (NP-40 extractable and nucleus-bound) and associates in vivo with MCM2 and MCM7 as a heteromeric complex. Co-immunoprecipitation, subcellular fractionation, cell cycle synchronization experiments Genes to cells : devoted to molecular & cellular mechanisms Medium 9286856
1998 Human MCM6 protein associates with chromatin in a multiprotein complex containing other Mcm proteins; chromatin-bound MCM6 is released by micrococcal nuclease digestion, and some MCM6 also occurs as a monomer on chromatin. Micrococcal nuclease digestion of chromatin, fractionation, antibody-based detection The Journal of biological chemistry Medium 9516426
2006 Phosphorylation of MCM4 at Thr-19 and Thr-110 by CDK2/cyclin A or EBV protein kinase inactivates MCM4-MCM6-MCM7 complex-associated DNA helicase activity; EBV-PK can also phosphorylate MCM6 itself to further inhibit helicase activity. In vitro helicase assay, site-directed mutagenesis of MCM4 N-terminal residues, kinase assay with CDK2/cyclin A and EBV-PK Journal of virology High 17005684
2009 Saccharomyces cerevisiae Mcm6 directly binds the checkpoint mediator Mrc1 through its 168-amino-acid C-terminal region; this interaction is required for replication checkpoint activation in response to methyl methanesulfonate (MMS) but not hydroxyurea. Physical fusion of Mcm6 mutant with Mrc1 suppresses the checkpoint defect. Protein interaction mapping, two amino acid substitution mutants abolishing Mrc1 binding, in vivo checkpoint assays, genetic suppression by fusion protein Molecular and cellular biology High 19620285
2010 The Mcm6/2 and Mcm5/3 ATPase active sites (Walker B box and arginine finger motifs) contribute to the function of the putative Mcm2/5 'gate' in the Mcm2-7 ring; mutational analysis shows these sites modulate overall helicase activity differentially. Walker B and arginine finger mutagenesis in S. cerevisiae Mcm2-7, ATPase and helicase functional assays Nucleic acids research High 20484375
2010 Chromatin-bound MCM6 exists in two distinct states in G1 phase (low and high levels); cells with high chromatin-bound MCM6 are in late G1 past the restriction point and cycle with committed phases (S, G2, M), linking MCM6 loading level to cell cycle commitment. Multivariate flow cytometry measuring chromatin-bound Mcm6, PCNA, DNA content, and mitotic marker; serum starvation/release and stathmokinetic experiments BMC cell biology Medium 20398392
2021 BLM helicase directly and physically interacts with MCM6: BLM binds the N-terminal domain of Mcm6 in G1 and switches to the C-terminal Cdt1-binding domain of Mcm6 in S-phase. Disruption of BLM/Mcm6 interaction leads to supra-normal DNA replication speed and delays repair of replication-dependent DSBs, establishing BLM as a negative regulator of replication speed via Mcm6. Co-IP of BLM complex components, domain-specific binding site mapping, replication speed assays, DNA damage sensitivity assays upon BLM-Mcm6 interaction disruption Nucleic acids research High 34370039
2022 YAP-TEAD complex binds the MCM6 promoter to induce MCM6 transcription in gastric cancer; MCM6 in turn activates PI3K/Akt/GSK3β signaling to support YAP-potentiated tumorigenicity. MCM6 deficiency blocks ATR/Chk1-mediated DNA damage response. ChIP-PCR, luciferase reporter assays, RNA-seq, knockdown/overexpression in vitro and xenograft mouse models, patient-derived organoids Theranostics High 36185598
2010 NMR chemical shift assignments define the Cdt1-binding domain (CBD) of human MCM6 as having a winged-helix fold; the CBD directly interacts with Cdt1 and likely mediates chromatin loading of the MCM2-7 complex. Triple-resonance NMR spectroscopy for backbone chemical shift assignments of human MCM6 CBD Biomolecular NMR assignments Medium 20623209
2024 Avermectin B1a occupies the CDT1-binding domain (CBD) of MCM6 (hydrogen bond contacts at Glu763, Ile760, Arg771, Glu774) and blocks the MCM6-CDT1 interaction, thereby inhibiting DNA replication licensing, causing G0/G1 arrest and apoptosis; upregulation of MCM6 or CDT1 reverses these effects. Molecular docking, ELISA-based binding assay, cell cycle analysis, rescue experiments with MCM6/CDT1 overexpression Environmental pollution Medium 39579921
2024 SIRT7-mediated lysine crotonylation of MCM6 at K599 (MCM6-K599cr) is upregulated in response to DNA replication stress, primarily due to disassembly of the MCM2-7 complex; this modification is regulated by RNF8-mediated ubiquitination. Mass spectrometry-based PTM identification, western blot for Kcr, RNF8 knockdown/overexpression, SIRT7 manipulation, ubiquitination assay Cell proliferation Medium 39477811
2024 RNF125 interacts with MCM6 and mediates its ubiquitination; RNF125 promotes HCC cell proliferation mainly through MCM6, establishing RNF125 as an E3 ubiquitin ligase regulating MCM6 protein homeostasis. Pull-down, co-immunoprecipitation, ubiquitination assay, co-transfection rescue experiments Oncology letters Medium 38298426
2023 MCM6 promotes intrahepatic cholangiocarcinoma progression by upregulating E2F1, which in turn mediates epithelial-mesenchymal transition (EMT); E2F1 knockdown partially blocks the pro-malignant effects of MCM6 overexpression. GSEA, MCM6 knockdown/overexpression, E2F1 knockdown epistasis experiments, in vitro and in vivo models Carcinogenesis Medium 37185675
2023 De novo MCM6 missense variant p.(Cys158Tyr) affecting a zinc-binding cysteine in the MCM6 zinc finger domain (essential for MCM complex dimerization and helicase activity induction) causes defects in ciliogenesis and cell proliferation in patient-derived fibroblasts. Trio exome/genome sequencing, patient fibroblast functional assays for ciliogenesis and cell proliferation Human genetics Medium 37198333
2021 LINC00472 recruits DNA methyltransferases to the MCM6 promoter to induce site-specific DNA methylation and reduce MCM6 expression; restoration of MCM6 weakens the tumor-suppressive effect of LINC00472, establishing epigenetic silencing of MCM6 by LINC00472 as a mechanism suppressing TNBC metastasis via MEK/ERK pathway. LINC00472 overexpression, MCM6 rescue experiments, DNA methylation assay at MCM6 promoter, in vitro and in vivo tumor models Aging Medium 33668040
2023 MCM6 expression in keratinocytes is governed by the IL-22/STAT3 pathway; benvitimod inhibits p-JAK1/p-JAK2 to restrain STAT3 activation and thereby reduces MCM6 expression, suppressing keratinocyte proliferation in psoriasis. MCM6 knockdown, IL-22/STAT3 pathway inhibition, JAK inhibitor experiments, in vitro HaCaT cells and in vivo IMQ-induced mouse model Journal of dermatological science Medium 36774328
2023 UBE3A (E6AP ubiquitin ligase) interacts with MCM6 (and MCM2/4), identified by yeast two-hybrid; E6AP knockout enhances ubiquitination of MCM2/4/6 but E6AP is not their E3 ligase. MCM6 and E6AP synergistically regulate proliferation and migration of lung adenocarcinoma cells. Yeast two-hybrid, co-immunoprecipitation, ubiquitination assay, combined knockdown proliferation/migration assays, xenograft mouse model FEBS open bio Medium 37454373
2025 MCM6 knockdown reduces nuclear c-Myc levels and promotes its ubiquitin-mediated degradation; c-Myc as a transcription factor binds the MCM6 promoter and promotes MCM6 transcription, forming a positive feedback loop that drives cisplatin resistance via enhanced DNA damage repair. MCM6 knockdown, western blot for c-Myc, ubiquitination assay, c-Myc ChIP at MCM6 promoter, cisplatin sensitivity assays in vitro and in vivo International journal of biological macromolecules Medium 39805445

Source papers

Stage 0 corpus · 56 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1999 Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity. Molecular and cellular biology 180 10567526
1999 Cell growth-regulated expression of mammalian MCM5 and MCM6 genes mediated by the transcription factor E2F. Oncogene 127 10327050
2002 Mouse geminin inhibits not only Cdt1-MCM6 interactions but also a novel intrinsic Cdt1 DNA binding activity. The Journal of biological chemistry 116 12192004
2018 MCM family in HCC: MCM6 indicates adverse tumor features and poor outcomes and promotes S/G2 cell cycle progression. BMC cancer 105 29463213
1999 Association of fission yeast Orp1 and Mcm6 proteins with chromosomal replication origins. Molecular and cellular biology 85 10490657
2008 Expression patterns and cell cycle profiles of PCNA, MCM6, cyclin D1, cyclin A2, cyclin B1, and phosphorylated histone H3 in the developing mouse retina. Developmental dynamics : an official publication of the American Association of Anatomists 67 18265020
2018 MCM6 promotes metastasis of hepatocellular carcinoma via MEK/ERK pathway and serves as a novel serum biomarker for early recurrence. Journal of experimental & clinical cancer research : CR 58 29357919
2009 The direct binding of Mrc1, a checkpoint mediator, to Mcm6, a replication helicase, is essential for the replication checkpoint against methyl methanesulfonate-induced stress. Molecular and cellular biology 55 19620285
2021 The DNA replication regulator MCM6: An emerging cancer biomarker and target. Clinica chimica acta; international journal of clinical chemistry 54 33609557
2006 Phosphorylation of MCM4 at sites inactivating DNA helicase activity of the MCM4-MCM6-MCM7 complex during Epstein-Barr virus productive replication. Journal of virology 54 17005684
2011 A single subunit MCM6 from pea promotes salinity stress tolerance without affecting yield. Plant molecular biology 52 21365356
2012 Wolbachia-induced aae-miR-12 miRNA negatively regulates the expression of MCT1 and MCM6 genes in Wolbachia-infected mosquito cell line. PloS one 49 23166816
2010 The Saccharomyces cerevisiae Mcm6/2 and Mcm5/3 ATPase active sites contribute to the function of the putative Mcm2-7 'gate'. Nucleic acids research 47 20484375
2022 MCM6 is a critical transcriptional target of YAP to promote gastric tumorigenesis and serves as a therapeutic target. Theranostics 38 36185598
2012 Expression of minichromosome maintenance MCM6 protein in meningiomas is strongly correlated with histologic grade and clinical outcome. The American journal of surgical pathology 33 22020044
1997 HsMCM6: a new member of the human MCM/P1 family encodes a protein homologous to fission yeast Mis5. Genes to cells : devoted to molecular & cellular mechanisms 31 9286856
2017 Minichromosome maintenance complex component 6 (MCM6) expression correlates with histological grade and survival in endometrioid endometrial adenocarcinoma. Virchows Archiv : an international journal of pathology 29 29243125
2006 The fertilization-induced DNA replication factor MCM6 of maize shuttles between cytoplasm and nucleus, and is essential for plant growth and development. Plant physiology 28 16407440
2004 Minichromosome maintenance protein (MCM6) in low-grade chondrosarcoma: distinction from enchondroma and identification of progressive tumors. American journal of clinical pathology 26 15539383
1996 Characterisation of a human homologue of a yeast cell division cycle gene, MCM6, located adjacent to the 5' end of the lactase gene on chromosome 2q21. FEBS letters 26 8977093
2021 MCM6 indicates adverse tumor features and poor outcomes and promotes G1/S cell cycle progression in neuroblastoma. BMC cancer 25 34233647
1998 Human protein MCM6 on HeLa cell chromatin. The Journal of biological chemistry 24 9516426
2021 Methylation-dependent MCM6 repression induced by LINC00472 inhibits triple-negative breast cancer metastasis by disturbing the MEK/ERK signaling pathway. Aging 22 33668040
2010 A single subunit MCM6 from pea forms homohexamer and functions as DNA helicase. Plant molecular biology 21 20730596
1996 A novel human Mcm protein: homology to the yeast replication protein Mis5 and chromosomal location. Genomics 19 8921380
2024 In silico functional, structural and pathogenicity analysis of missense single nucleotide polymorphisms in human MCM6 gene. Scientific reports 14 38773180
2020 The impact of MCM6 on hepatocellular carcinoma in a Southern Chinese Zhuang population. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 14 32403044
2021 A novel cell-cycle-regulated interaction of the Bloom syndrome helicase BLM with Mcm6 controls replication-linked processes. Nucleic acids research 12 34370039
2010 Cytometry of chromatin bound Mcm6 and PCNA identifies two states in G1 that are separated functionally by the G1 restriction point. BMC cell biology 12 20398392
1998 cDNA cloning and expression during development of Drosophila melanogaster MCM3, MCM6 and MCM7. Gene 12 9795205
2011 Promoter of a salinity and cold stress-induced MCM6 DNA helicase from pea. Plant signaling & behavior 11 21691155
2023 Benvitimod inhibits MCM6-meditated proliferation of keratinocytes by regulating the JAK/STAT3 pathway. Journal of dermatological science 10 36774328
2022 MCM6 versus Ki-67 in diagnosis of luminal molecular subtypes of breast cancers. Diagnostic pathology 10 35125121
2024 Crotonylation of MCM6 enhances chemotherapeutics sensitivity of breast cancer via inducing DNA replication stress. Cell proliferation 9 39477811
2023 Hypoxia Induces Tumor-Derived Exosome SNHG16 to Mediate Nasopharyngeal Carcinoma Progression through the miR-23b-5p/MCM6 Pathway. Applied biochemistry and biotechnology 9 37119503
2007 Expression of human MCM6 and DNA Topo II alpha in craniopharyngiomas and its correlation with recurrence of the tumor. Journal of neuro-oncology 9 17410335
2023 MCM6 promotes intrahepatic cholangiocarcinoma progression by upregulating E2F1 and enhancing epithelial-mesenchymal transition. Carcinogenesis 8 37185675
2022 MCM6 Promotes Hepatocellular Carcinoma Progression via the Notch Pathway: Clinical, Functional, and Genomic Insights. Computational and mathematical methods in medicine 8 35720029
2012 Impact of salinity-tolerant MCM6 transgenic tobacco on soil enzymatic activities and the functional diversity of rhizosphere microbial communities. Research in microbiology 8 22989673
2023 UBE3A and MCM6 synergistically regulate the proliferation and migration of lung adenocarcinoma cells. FEBS open bio 6 37454373
2024 RNF125‑mediated ubiquitination of MCM6 regulates the proliferation of human liver hepatocellular carcinoma cells. Oncology letters 5 38298426
2015 Development of stapled helical peptides to perturb the Cdt1-Mcm6 interaction. Journal of peptide science : an official publication of the European Peptide Society 5 25921752
2014 Are there differences between patients with and without the homozygous--13910CC genetic variant in the MCM-6 gene upstream from the lactase gene?--A non-randomised, two armed intervention study without control group. Clinical laboratory 4 25651706
2024 Pesticide avermectin B1a exerts cytotoxicity by blocking the interaction between mini-chromosome maintenance 6 protein (MCM6) and chromatin licensing and DNA replication factor 1 (CDT1). Environmental pollution (Barking, Essex : 1987) 3 39579921
2023 De novo MCM6 variants in neurodevelopmental disorders: a recognizable phenotype related to zinc binding residues. Human genetics 3 37198333
2025 Roles of MARCKSL1, MCM6, RFC4, and PLAU genes in esophageal cancer and their association with radiotherapy response. Scientific reports 2 40603489
2023 Genome-wide binding sites of Plasmodium falciparum mini chromosome maintenance protein MCM6 show new insights into parasite DNA replication. Biochimica et biophysica acta. Molecular cell research 2 37482133
2011 Inhibition of unwinding and ATPase activities of pea MCM6 DNA helicase by actinomycin and nogalamycin. Plant signaling & behavior 2 21336027
2025 The MCM6-c-Myc positive feedback loop mediates bladder cancer progression and cisplatin resistance. International journal of biological macromolecules 1 39805445
2026 Frequency and Diagnostic Utility of the -13910C>T MCM6 Gene Polymorphism for Lactose Intolerance in a Brazilian Northeast Population. In vivo (Athens, Greece) 0 41482367
2025 Antiparasitic agent emodepside exerts cytotoxicity in human corneal stromal cells by blocking the interaction between mini-chromosome maintenance 6 protein (MCM6) and chromatin licensing and DNA replication factor 1 (CDT1). Toxicology 0 41203057
2025 Targeting MCM6 Enhances Melphalan Chemosensitivity in Retinoblastoma by Modulating DNA Damage Response. Investigative ophthalmology & visual science 0 41334961
2025 TCF19 promotes gastric cancer progression through MCM6. BMC gastroenterology 0 41398215
2024 MCM6 Inhibits Decidualization via Cross-Talking with ERK Pathway in Human Endometrial Stromal Cells. Reproductive sciences (Thousand Oaks, Calif.) 0 38347378
2023 Retracted: MCM6 Promotes Hepatocellular Carcinoma Progression via the Notch Pathway: Clinical, Functional, and Genomic Insights. Computational and mathematical methods in medicine 0 37475947
2010 1H, 15N and 13C chemical shift assignments of the Cdt1 binding domain of human Mcm6. Biomolecular NMR assignments 0 20623209