Affinage

DDX5

Probable ATP-dependent RNA helicase DDX5 · UniProt P17844

Length
614 aa
Mass
69.1 kDa
Annotated
2026-06-09
100 papers in source corpus 51 papers cited in narrative 51 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

DDX5 is a multifunctional DEAD-box RNA helicase that couples RNA/DNA structure remodeling to transcription, RNA processing, and genome stability (PMID:31267554, PMID:31548374). It resolves R-loops (RNA:DNA hybrids) in an ATP-dependent manner at transcription termination sites and DNA double-strand breaks, associating with XRN2 to promote RNA polymerase II release; this activity depends on PRMT5-catalyzed arginine methylation of its RGG/RG motif, which licenses interaction with XRN2 and the scaffold Thrap3 but is dispensable for intrinsic helicase activity (PMID:31267554, PMID:34697388, PMID:32747416). At double-strand breaks DDX5 clears R-loops to enable homologous recombination, with its hybrid-unwinding activity stimulated by BRCA2 and its retention supported by partners including TOP3B and MCM8 (PMID:33634895, PMID:33015627, PMID:35830799, PMID:38858601). Beyond R-loops, DDX5 is a potent resolvase of DNA and RNA G-quadruplexes—unfolding the MYC promoter G4 to activate transcription and the STAT1 5'UTR rG4 to license translation and interferon signaling (PMID:31548374, PMID:34021034). DDX5 functions broadly as a transcriptional co-regulator, co-activating nuclear receptors and transcription factors including RORγt (requiring helicase activity and the lncRNA Rmrp), β-catenin, androgen receptor, Runx2, c-Myc, RBP-J/Notch, and Fra-1, frequently by recruiting RNA polymerase II to target promoters (PMID:26675721, PMID:23396200, PMID:26212035, PMID:24469041, PMID:23349811, PMID:31015574). It governs alternative pre-mRNA splicing—cooperating with hnRNP H/F to set differentiation-specific splicing programs and controlling splicing of substrates such as H-Ras, GSK3β, CaMKIIδ, and IL-36R in tissue-specific physiology and disease (PMID:24910439, PMID:18698352, PMID:24275493, PMID:39056171, PMID:36271146). DDX5 additionally directs miRNA biogenesis through the Drosha/DGCR8 Microprocessor (PMID:34936874, PMID:28111200) and modulates mRNA fate via the METTL3/METTL14 m6A writer complex, shaping transcript stability and innate immune signaling (PMID:33909701, PMID:38182816). DDX5 abundance and activity are tuned post-translationally by PRMT5 methylation, PAK5-driven phosphorylation and sumoylation, and HSP90-dependent protection from autophagic degradation (PMID:31267554, PMID:34936874, PMID:33764710). Genetically, cardiomyocyte-specific Ddx5 loss causes heart failure through misregulated CaMKIIδ splicing and disrupted Ca2+ homeostasis (PMID:39056171).

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 2007 Medium

    Established that DDX5 contributes to ribosome biogenesis, identifying an early role in pre-rRNA processing through RNA rearrangement rather than canonical unwinding.

    Evidence siRNA co-silencing of DDX5/DDX17 with rRNA processing and nucleolar morphology readouts and helicase mutant analysis

    PMID:17485482

    Open questions at the time
    • Redundancy with DDX17 obscures DDX5-specific contribution
    • Molecular nature of the RNA rearrangement activity undefined
  2. 2013 Medium

    Defined DDX5 as a versatile transcriptional co-activator and splicing/export regulator for diverse factors, broadening its role beyond RNA metabolism into gene-specific transcription.

    Evidence Co-IP, ChIP, reporter assays, and RNA-seq across β-catenin/AR, Notch RBP-J, c-Myc, VDR, ERα/AR, H-Ras splicing, and c-fos export systems

    PMID:18698352 PMID:22476084 PMID:23143267 PMID:23349811 PMID:23396200 PMID:24275493 PMID:24469041

    Open questions at the time
    • Whether helicase activity is required differs by partner and is not uniformly resolved
    • Direct vs. bridged interactions not always distinguished
  3. 2014 High

    Showed DDX5 cooperates with hnRNP H/F to set differentiation-specific alternative splicing subprograms, linking it to coordinated cell-state transitions.

    Evidence RNA-seq after knockdown, Co-IP with hnRNP H/F, and miRNA profiling in myogenesis and EMT models

    PMID:24910439

    Open questions at the time
    • Direct RNA targets vs. indirect effects not fully separated
    • Mechanism coupling splicing to miRNA-mediated DDX5 downregulation incomplete
  4. 2015 High

    Demonstrated that DDX5 co-activation can require both helicase activity and a specific lncRNA cofactor, establishing a paradigm for RNA-guided transcription factor co-activation.

    Evidence Reciprocal Co-IP of DDX5-RORγt, helicase mutants, and a cartilage-hair-hypoplasia Rmrp mutation in mice with TH17 phenotypes

    PMID:26675721

    Open questions at the time
    • How Rmrp binding mechanistically enables co-activation unknown
    • Generalizability of lncRNA-dependence to other DDX5 partners untested
  5. 2019 High

    Resolved the dual structural-substrate identity of DDX5, showing it both resolves R-loops at termination sites (methylation- and XRN2-dependent) and unfolds G-quadruplexes to control transcription.

    Evidence In vitro R-loop and G4 unfolding with recombinant protein, RGG/RG and ATP-decoupling mutagenesis, DRIP-qPCR, Co-IP, and ChIP-seq

    PMID:31267554 PMID:31548374

    Open questions at the time
    • Whether the same DDX5 molecules switch between R-loop and G4 substrates in vivo unknown
    • Determinants of substrate selection not defined
  6. 2020 High

    Placed DDX5 in the DNA double-strand break response, showing R-loop clearance near breaks is needed for homologous recombination and genome stability.

    Evidence CLIP, ChIP, DRIP-seq, NHEJ reporter, laser-induced foci, and EXO1/RPA recruitment in DDX5-deficient cells; genome-wide DRIP-seq comparison with XRN2/PRMT5

    PMID:32747416 PMID:33015627

    Open questions at the time
    • DDX5's distinct role at transcription start sites mechanistically unexplained
    • How DDX5 is excluded from breaks in an ATM-dependent manner unresolved
  7. 2021 High

    Identified upstream regulators and partners that stimulate or scaffold DDX5's R-loop activity, including BRCA2, Thrap3, TOP3B, and MCM8, embedding DDX5 in a genome-protective network.

    Evidence In vitro helicase stimulation, Co-IP, R-loop/DRIP assays, disease-variant analysis (BRCA2-T207A, MCM8 POI mutants)

    PMID:33634895 PMID:34697388 PMID:35830799 PMID:38858601

    Open questions at the time
    • Hierarchy and temporal order among these partners at a given R-loop unclear
    • Whether interactions are direct in all cases not fully established
  8. 2021 Medium

    Connected DDX5 to m6A-dependent mRNA fate, showing it shapes the METTL3/METTL14 writer complex to control transcript methylation, export, and decay in innate immunity.

    Evidence Co-IP of DDX5-METTL3/14, m6A-seq, mRNA stability and export assays, YTHDF2 decay readouts, and in vivo infection KO models

    PMID:29522752 PMID:33909701 PMID:38182816

    Open questions at the time
    • Whether DDX5 directly modulates METTL3 catalytic activity or substrate access unresolved
    • Context-dependent promotion vs. inhibition of m6A across studies not reconciled
  9. 2021 Medium

    Established post-translational control of DDX5 abundance through phosphorylation/sumoylation and chaperone-mediated stabilization, linking signaling to DDX5-dependent miRNA processing and oncogenesis.

    Evidence PAK5 kinase assay (T69), sumoylation and DDX5/Drosha/DGCR8 Co-IP, HSP90 Co-IP and autophagy pathway analysis, in vivo tumor models

    PMID:33764710 PMID:34936874

    Open questions at the time
    • Quantitative contribution of each modification to steady-state DDX5 unknown
    • Interplay between stabilization and helicase activity untested
  10. 2024 High

    Provided in vivo causal evidence that DDX5-controlled alternative splicing maintains tissue homeostasis, with cardiomyocyte loss causing heart failure via CaMKIIδ missplicing and Ca2+ dysregulation.

    Evidence Cardiomyocyte-specific KO, AAV9 rescue of CaMKIIδA, IP-MS, RNA-seq, RIP-seq, and Ca2+ transient measurements; parallel cartilage KO defining splicing and G4 mechanisms

    PMID:38760576 PMID:39056171

    Open questions at the time
    • Full repertoire of physiologically critical DDX5 splicing substrates per tissue undefined
    • Whether helicase/G4 vs. splicing functions dominate phenotypes is tissue-specific and not generalized

Open questions

Synthesis pass · forward-looking unresolved questions
  • How DDX5 selects among its many activities—R-loop resolution, G4 unwinding, splicing, transcriptional co-regulation, and m6A modulation—at a given locus and how its post-translational modifications and RNA cofactors integrate to dictate this choice remain unresolved.
  • No unified model linking modification state to functional output
  • Structural basis for substrate discrimination unknown
  • Partner-specific requirement for helicase activity not systematically mapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 6 GO:0003723 RNA binding 5 GO:0140098 catalytic activity, acting on RNA 5 GO:0098772 molecular function regulator activity 3 GO:0140657 ATP-dependent activity 3
Localization
GO:0005634 nucleus 4 GO:0005829 cytosol 4 GO:0000228 nuclear chromosome 2 GO:0005730 nucleolus 2
Pathway
R-HSA-168256 Immune System 5 R-HSA-74160 Gene expression (Transcription) 5 R-HSA-8953854 Metabolism of RNA 5 R-HSA-73894 DNA Repair 3 R-HSA-1852241 Organelle biogenesis and maintenance 2
Partners
BRCA2CTNNB1MCM8METTL3MYCTHRAP3TOP3BXRN2
Complex memberships
Drosha/DGCR8 MicroprocessorMETTL3-METTL14 m6A writer complex

Evidence

Reading pass · 51 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2019 DDX5 resolves R-loops (RNA:DNA hybrids) in an ATP-dependent manner in vitro; its RGG/RG motif is methylated by PRMT5, and this methylation is required for DDX5 interaction with XRN2 and repression of cellular R-loops (but not for helicase enzymatic activity itself). DDX5 associates with XRN2 at transcriptional termination regions to facilitate RNA polymerase II release. In vitro R-loop resolution assay with recombinant DDX5, co-immunoprecipitation of DDX5-XRN2, DRIP-qPCR in DDX5-deficient cells, RGG/RG motif mutagenesis, identification of PRMT5 as the writer by binding/methylation assays The EMBO journal High 31267554
2021 BRCA2 physically interacts with DDX5 and stimulates its DNA-RNA hybrid-unwinding helicase activity at DNA double-strand breaks (DSBs). The BRCA2-T207A breast cancer variant shows impaired interaction with DDX5, reducing DDX5 association with DNA-RNA hybrids near DSBs and altering RPA and RAD51 recruitment kinetics. Co-immunoprecipitation identifying DDX5 as BRCA2-interacting protein, in vitro helicase stimulation assay, DRIP at DSBs, analysis of BRCA2-T207A variant cells, RPA/RAD51 foci quantification The EMBO journal High 33634895
2015 DDX5 functions as a transcriptional co-activator of the nuclear receptor RORγt in TH17 cells; this co-activation requires DDX5's intrinsic RNA helicase activity and binding of the lncRNA Rmrp. A Rmrp mutation corresponding to cartilage-hair hypoplasia reduced DDX5-RORγt interaction and RORγt target gene transcription. Co-immunoprecipitation of DDX5-RORγt complex, RNA helicase activity mutants, targeted Rmrp gene mutation in mice, chromatin occupancy studies, TH17 differentiation and inflammatory disease models Nature High 26675721
2019 DDX5 is a highly active G-quadruplex (G4) resolvase that unfolds MycG4-DNA without requiring a single-stranded overhang; ATP hydrolysis is not directly coupled to G4-unfolding. DDX5 is enriched at G-rich chromatin sites including the MYC promoter and activates MYC transcription; G4-interactive small molecules inhibit DDX5's interaction with the MYC promoter. In vitro G4-unfolding assays, ATP hydrolysis decoupling experiments, ChIP-seq for DDX5 chromatin binding, MYC transcription assays, G4-stabilizing small molecule experiments Proceedings of the National Academy of Sciences of the United States of America High 31548374
2020 DDX5 clears R-loops at or near DSBs to enable proper DNA repair. DDX5 binds RNA transcripts near DSBs (shown by CLIP), requires its helicase domain, is excluded from DSBs in a transcription- and ATM activation-dependent manner, and its deficiency leads to asymmetric chromosomal deletions and impaired homologous recombination (delayed EXO1 and RPA recruitment). CLIP (crosslinking immunoprecipitation), ChIP, DRIP-seq in DDX5-deficient cells, NHEJ reporter (EJ5-GFP), laser irradiation-induced damage foci, EXO1/RPA recruitment assays NAR cancer High 33015627
2022 TOP3B resolves R-loops in coordination with DDX5 independently of TDRD3. IP-mass spectrometry and IP-western show TOP3B physically interacts with DDX5. DDX5 and TOP3B are epistatic in resolving R-loops in a pathway parallel with senataxin; TOP3B cleaves the single-stranded DNA displaced by R-loop RNA-DNA duplexes. IP-mass spectrometry, IP-western blotting, biochemical assays with recombinant TOP3B and oligonucleotide R-loop mimics, RNA/DNA hybrid IP-western, genetic epistasis Cell reports High 35830799
2021 DDX5 interacts with the m6A writer METTL3 to regulate methylation of mRNAs including DHX58, p65, and IKKγ by affecting the METTL3-METTL14 heterodimer complex; DDX5 promotes m6A modification and nuclear export of these transcripts, leading to YTHDF2-dependent mRNA decay of antiviral transcripts and suppression of IFN-β signaling. Co-immunoprecipitation of DDX5-METTL3 complex, m6A sequencing, mRNA stability assays, nuclear export assays, viral infection models in vitro and in vivo PLoS pathogens Medium 33909701
2022 DDX5 interacts with METTL3 and METTL14 to form an m6A writing complex that adds m6A to TLR2 and TLR4 transcripts, promoting their YTHDF2-mediated decay; upon bacterial infection, DDX5 is recruited to Hrd1 at the ER in a MyD88-dependent manner and degraded via the ubiquitin-proteasome pathway, disrupting the complex and allowing TLR2/4 upregulation and NF-κB activation. Co-immunoprecipitation of DDX5-METTL3/14 complex, m6A modification assays, YTHDF2 mRNA decay assays, bacterial infection models, MyD88-dependent recruitment assay, in vivo DDX5-KO and METTL3-KO mice EMBO reports High 38182816
2021 DDX5 resolves a G-quadruplex structure (rG4) in the 5'UTR of STAT1 mRNA to enable STAT1 translation and interferon signaling. Direct and selective binding of helicase-active DDX5 to the WT STAT1-rG4 sequence was demonstrated by ribonucleoprotein and EMSA assays; CRISPR editing of the STAT1-rG4 sequence conferred resistance to rG4-stabilizing compounds. Luciferase reporter assays with WT vs. mutant rG4 sequence, rG4-stabilizing compounds, CRISPR/Cas9 editing of STAT1-rG4, circular dichroism, RNP/EMSA binding assays, IFN-α response assays Gut High 34021034
2014 DDX5 and DDX17 cooperate with hnRNP H/F splicing factors to define epithelial- and myoblast-specific splicing subprograms during differentiation. DDX5/DDX17 downregulation during myogenesis and EMT contributes to splicing program switching; this downregulation is itself mediated by DDX5/DDX17-dependent miRNA production. RNA-seq of splicing events after knockdown, co-immunoprecipitation with hnRNP H/F, miRNA profiling, cell differentiation models Cell reports High 24910439
2007 DDX5 (p68) and its paralog DDX17 (p72/p82) are required for 32S pre-rRNA cleavage and ribosome biogenesis; their apparently redundant role corresponds to RNA rearrangement (not unwinding) activity required for structural rearrangement within the pre-60S ribosomal subunit preceding 32S processing. Co-silencing of both genes causes nucleolar structure perturbation and cell death. siRNA knockdown of DDX5 and/or DDX17, RNA helicase mutant studies, nucleolar morphology analysis, pre-rRNA processing assays Nucleic acids research Medium 17485482
2011 ARF limits the nucleolar localization of DDX5 by inhibiting the interaction between DDX5 and nucleophosmin (NPM), preventing DDX5 association with the rDNA promoter and nuclear pre-ribosomes. DDX5 promotes rRNA synthesis/maturation and ribosome output. Mass spectrometry of nucleolar proteins in Arf-deficient cells, Co-immunoprecipitation of DDX5-NPM, ChIP at rDNA promoter, DDX5 knockdown effects on ribosome biogenesis Cancer research Medium 21937682
2012 DDX5 promotes DNA replication by directly regulating E2F-dependent gene promoters (recruiting RNA polymerase II to E2F-regulated gene promoters) to control DNA replication factor expression during G1-S phase progression. Episomal plasmid stability assay, ChIP for RNA Pol II at E2F promoters, DDX5 knockdown effects on replication factor expression, cell cycle analysis Cancer discovery Medium 22750847
2008 DDX5 (p68) functions as a transcriptional co-activator of Runx2 in osteoblasts; p68 co-localizes with Runx2 in nuclear punctate foci. Helicase activity was not essential for Runx2 co-activation. Paradoxically, p68 suppression accelerated osteoblast differentiation, and Runx2 suppressed p68 expression in calvarial progenitors, revealing reciprocal regulation. Affinity purification/proteomics identifying p68-Runx2 interaction, co-localization by immunofluorescence, transcription reporter assays with helicase mutants, siRNA knockdown, osteoblast differentiation assays Journal of cellular biochemistry Medium 17960593
2013 DDX5 (p68) functions as a co-activator in the Notch signaling pathway by directly interacting with RBP-J. DDX5 localizes to RBP-J binding sites at Notch target gene promoters (preTCRα, Hes1, CD25) in a Notch-dependent manner. The RNA co-activator SRA acts as a DDX5 cofactor in this context; DDX5/SRA co-activation is accompanied by p300 occupancy and histone acetylation. Biotinylation-tagging followed by mass spectrometry identifying DDX5 in RBP-J/NICD complex, biochemical interaction assays, ChIP at Notch target gene loci, siRNA knockdown, SRA knockdown/overexpression Biochimica et biophysica acta Medium 23396200
2013 DDX5 (p68) unwinds the stem-loop IDX-rasISS1 structure in H-Ras pre-mRNA and prevents binding of hnRNP H to this structure, thereby regulating alternative splicing of H-Ras to modulate the ratio of p21/p19 H-Ras isoforms. p68 also alters dynamic localization of SC35 splicing factor. In vitro RNA unwinding assays, RNA-protein binding competition assays, SC35 localization by immunofluorescence, RNAi knockdown PloS one Medium 18698352
2012 DDX5 binds the lncRNA mrhl RNA in mouse spermatogonial cells (identified by Northwestern blot and RNA pulldown); mrhl RNA downregulation causes cytoplasmic translocation of tyrosine-phosphorylated DDX5 (p68). Concomitant knockdown of both mrhl RNA and p68 prevented nuclear β-catenin translocation, placing DDX5 downstream of mrhl RNA in negative regulation of Wnt signaling. Northwestern blot, RNA pulldown identifying DDX5 as mrhl RNA binding protein, co-knockdown epistasis, β-catenin localization assays, TOP/FOP luciferase assay Molecular and cellular biology Medium 22665494
2015 DDX5 directly interacts with β-catenin, promotes its nuclear translocation, and co-activates expression of cyclin D1 and c-Myc in NSCLC cells. β-catenin silencing abrogates DDX5-induced expression of these targets. Co-immunoprecipitation of DDX5-β-catenin, β-catenin nuclear localization assay, siRNA epistasis for cyclin D1/c-Myc expression, luciferase reporter assays Cancer science Medium 26212035
2013 DDX5 (Ddx5) and DDX17 (ddx17) act as dual transcriptional coactivators of NFAT5 and simultaneously promote inclusion of NFAT5 exon 5, which introduces a premature termination codon triggering NMD of NFAT5 mRNA, thereby reducing NFAT5 protein level while enhancing NFAT5 transcriptional activity. Transcription reporter assays, alternative splicing analysis (RT-PCR), NMD pathway validation, siRNA knockdown, cell migration assays Oncogene Medium 22266867
2013 DDX5 interacts with DDX3 (identified by yeast two-hybrid and confirmed by Co-IP); the interaction is enhanced in the G2/M phase when DDX5 accumulates in the cytoplasm. Dephosphorylation of serine/threonine residues in both DDX3 and DDX5 enhances their interaction (demonstrated by PP2A treatment). DDX3 knockdown blocks nuclear shuttling of DDX5, and both proteins are involved in mRNP export (UV cross-linking). Yeast two-hybrid, Co-immunoprecipitation, cell-cycle phase fractionation, PP2A/PTP1B phosphatase treatment, GST pulldown, UV cross-linking, siRNA knockdown with nuclear localization readout Journal of cellular biochemistry Medium 22034099
2013 DDX5 binds hUpf3 (a component of the exon junction complex/NMD machinery) and activates NMD of Ddx17/p72 and Smg5 mRNAs. ATP-binding activity of DDX5 and the 3'UTR of target mRNAs are required for NMD triggering. DDX5 binding to Upf3 interferes with EJC binding. Co-immunoprecipitation of DDX5-Upf3, NMD reporter assays, ATP-binding mutant analysis, 3'UTR requirement assays Nucleic acids research Medium 23788676
2014 DDX5 interacts with c-Myc and is required for c-Myc-mediated transcription and transforming activity. ARF blocks the physical interaction between DDX5 and c-Myc and displaces DDX5 from c-Myc target gene promoters. Forced c-Myc expression accelerates DDX5 protein synthesis, suggesting an oncogenic positive feedback loop. Tandem affinity purification identifying DDX5 as Arf-interacting protein, Co-immunoprecipitation of DDX5-c-Myc, ChIP at c-Myc target promoters, transcription assays, protein synthesis analysis Oncogene Medium 24469041
2009 DDX5 acts as a repressor of fibrogenic genes in hepatic stellate cells by interacting with transcriptional complexes at fibrogenic gene promoters. The S480A SNP reduces DDX5 homodimer recruitment to fibrogenic promoters, increasing fibrogenic gene expression and Smad3/AP-1-responsive reporter activities without altering HDAC1 recruitment. Transient overexpression/siRNA knockdown with SNP replacement, stable expression of WT vs. SNP DDX5, promoter reporter assays, GAL4 one-hybrid system, ChIP for DDX5 homodimers at promoters The Journal of biological chemistry Medium 20022962
2021 Thrap3 interacts with arginine-methylated DDX5 and co-localizes to R-loops. The Thrap3-DDX5 axis induces recruitment of XRN2 to R-loops; loss of Thrap3 increases R-loop accumulation and DNA damage. Arginine methylation of DDX5 is required for the DDX5-Thrap3 interaction. Co-immunoprecipitation of Thrap3-DDX5, R-loop localization assays, XRN2 recruitment assays, R-loop accumulation measurement in Thrap3-depleted cells, methylation requirement analysis Experimental & molecular medicine Medium 34697388
2021 PAK5 phosphorylates DDX5 at threonine 69; this phosphorylation promotes sumoylation of DDX5 (phosphorylation-dependent sumoylation), which stabilizes DDX5. Both phosphorylation and sumoylation enhance formation of a DDX5/Drosha/DGCR8 complex, promoting microRNA-10b processing and breast cancer proliferation/metastasis. Kinase assay identifying DDX5-T69 as PAK5 substrate, Co-immunoprecipitation of DDX5/Drosha/DGCR8, sumoylation assays, PAK5-/-/MMTV-PyVT transgenic mice, miR-10b processing assays Cell reports Medium 34936874
2017 DDX5 inhibits somatic cell reprogramming by repressing RYBP expression via processing of miR-125b. DDX5 disruption impedes miR-125b processing, leading to Rybp upregulation, H2AK119 ubiquitination by RYBP-dependent PRC1, and suppression of lineage genes. RYBP also mediates PRC1-independent OCT4 recruitment to the Kdm2b promoter. iPSC reprogramming efficiency assays in Ddx5 KO cells, miR-125b processing assays, RYBP overexpression/knockdown epistasis, H2AK119ub ChIP, OCT4 ChIP at Kdm2b promoter Cell stem cell Medium 28111200
2013 DDX5 functions as a cellular co-factor of HIV-1 Rev, facilitating export of unspliced viral mRNAs. DDX5 binds Rev in a largely RNA-dependent manner; mutation of the DEAD-box motif abolishes DDX5-Rev interaction, indicating the DEAD-box motif is required for this interaction. Co-immunoprecipitation and confocal microscopy of DDX5-Rev, DEAD-box motif mutagenesis, Rev-RRE functional assays, HIV replication assays PloS one Medium 23741449
2020 DDX5 potentiates HIV-1 transcription elongation as a co-factor of Tat; DDX5 binds both Tat and HEXIM1 and may facilitate dissociation of HEXIM1 from the 7SK-snRNP complex, enhancing Tat/P-TEFb availability. N-terminal RNA binding motifs, Walker B, and glycine doublet motifs of DDX5 are essential for this function. siRNA knockdown with DDX5 mutant rescue, Co-IP of DDX5-Tat and DDX5-HEXIM1, Tat/LTR reporter assays, HIV infectivity assays Retrovirology Medium 32228614
2020 DDX5 suppresses type I IFN production by interacting with PP2A-Cβ; viral infection enhances the DDX5-PP2A-Cβ interaction. PP2A-Cβ interacts with IRF3 and deactivates it (dephosphorylation); DDX5 knockdown promotes IRF3 phosphorylation and IFN-I production and renders mice more resistant to viral infection. Co-immunoprecipitation of DDX5-PP2A-Cβ and PP2A-Cβ-IRF3, IRF3 phosphorylation assays, IFN-β production measurement, siRNA knockdown, viral infection in vivo mouse model Experimental cell research Medium 33065113
2012 DDX5 is required for c-fos expression at multiple steps: transcriptional activation (recruited to c-fos gene upon estrogen), co-transcriptional RNA splicing, and mRNA export via efficient recruitment of the TAP mRNA export receptor. When splicing occurs post-transcriptionally in the absence of DDX5, c-fos mRNA is poorly exported. ChIP for DDX5 at c-fos gene locus, co-transcriptional splicing assays, TAP recruitment assays, mRNA export assays, DDX5 knockdown Nucleic acids research Medium 23143267
2013 DDX5 and DDX17 are master regulators of estrogen and androgen receptor signaling pathways, controlling transcription and splicing of downstream target genes and of upstream regulators including GSK3β; DDX5/DDX17 control alternative splicing of GSK3β kinase, impacting ER and AR protein stability. RNA-seq of splicing events after DDX5/DDX17 knockdown, transcription reporter assays, ChIP, GSK3β splicing and ER/AR stability analysis Nucleic acids research Medium 24275493
2013 DDX5 co-activates androgen receptor (AR)-dependent transcription in prostate cancer cells by forming a complex with nuclear β-catenin and recruiting AR and β-catenin to androgen-responsive promoters. DDX5 also co-immunoprecipitates with both processive and non-processive forms of RNA polymerase II and is found at elongating regions of the AR-mediated PSA gene. Co-immunoprecipitation of DDX5-β-catenin (nuclear fraction), ChIP at AR-responsive promoters, ChIP for elongating RNA Pol II, luciferase reporter assays, siRNA knockdown PloS one Medium 23349811
2019 DDX5 is identified as the most enriched endogenous chromatin-bound Fra-1 interacting protein in TNBC cells, showing extensive overlap with Fra-1 cistrome; DDX5 enhances Fra-1 transcriptional activity and potentiates Fra-1-driven cell proliferation. Endogenous interaction profiling (IP of chromatin-bound Fra-1 + mass spectrometry), ChIP-seq overlap analysis, DDX5 overexpression reporter assays, cell proliferation assays Oncogene Medium 31015574
2019 DDX5 acts as a transcriptional co-activator of PLZF (a transcription factor required for germline maintenance) in spermatogonia, regulating select target genes. DDX5 also regulates splicing of key spermatogenesis genes and controls cell cycle gene expression post-transcriptionally in undifferentiated spermatogonia. Inducible knockout mouse model, RNA-seq for splicing changes, Co-immunoprecipitation of DDX5-PLZF, target gene expression analysis Nature communications Medium 31123254
2022 DDX5 inhibits IL-17D-induced skin inflammation by regulating pre-mRNA splicing in keratinocytes; DDX5 loss shifts IL-36R splicing toward membrane-bound intact IL-36R at the expense of soluble IL-36R (sIL-36R). IL-17D downregulates DDX5 expression via the CD93-p38 MAPK-AKT-SMAD2/3 signaling pathway. Keratinocyte-specific Ddx5 knockout mice, alternative splicing analysis by RT-PCR, IL-17D signaling pathway inhibitors, sIL-36R restoration experiments, skin inflammation disease models Nature immunology Medium 36271146
2024 DDX5 regulates CaMKIIδ alternative splicing in cardiomyocytes to prevent production of CaMKIIδA isoform; DDX5 loss leads to CaMKIIδA accumulation, which phosphorylates L-type calcium channel (Cacna1c serine residues), impairing Ca2+ homeostasis and causing heart failure. AAV9-mediated CaMKIIδA knockdown partially rescues cardiac dysfunction in DDX5 KO mice. Cardiomyocyte-specific Ddx5 KO mice, AAV9 DDX5 overexpression, IP-mass spectrometry, RNA-seq, alternative splicing analysis, RNA immunoprecipitation sequencing, Ca2+ transient measurements, transverse aortic constriction HF model Circulation High 39056171
2024 DDX5 inhibits cartilage fibrosis in osteoarthritis by regulating alternative splicing of Fn1 and Plod2 pre-mRNAs and by unfolding G-quadruplex structures in the Col2 promoter to promote COL2 expression; loss of DDX5 increases fibrosis-related (Col1, Acta2) and cartilage-degrading enzyme genes (Mmp13, Nos2). Chondrocyte-specific Ddx5 KO mice in OA model, alternative splicing analysis, G-quadruplex unfolding assays, gene expression analysis Nature aging Medium 38760576
2018 DDX5 participates in oxLDL-induced macrophage MSR1 expression by stabilizing MSR1 mRNA; DDX5 interacts with METTL3 and inhibits METTL3-mediated m6A methylation of MSR1 mRNA, maintaining MSR1 mRNA stability and promoting lipid uptake. Co-immunoprecipitation of DDX5-METTL3, mass spectrometry identification, RNA-IP, dual luciferase reporter, mRNA stability assays (actinomycin D chase), siRNA knockdown Experimental cell research Medium 29522752
2020 DDX5 deficiency in intestinal epithelial cells protected mice from intestinal tumorigenesis and DSS-induced colitis; DDX5 binds C3 and Fabp1 mRNA transcripts and augments their expression post-transcriptionally in a tissue-specific manner to promote oncogenesis. Intestinal epithelial-specific DDX5 KO mice, RNA-binding protein immunoprecipitation, DSS colitis model, tumorigenesis models Life science alliance Medium 32817263
2023 DDX5 acts as a transcriptional co-repressor in RORγt+ Tregs, restricting expression of HIF1α and its downstream target IL-10; T cell-specific DDX5 knockout augments RORγt+ Treg suppressor activity and protects mice from intestinal inflammation. T cell-specific Ddx5 KO mice, IL-10 reporter assays, HIF1α inhibitor epistasis, intestinal inflammation model, transcriptomic analysis Science advances Medium 36724232
2012 DDX5 (p68) directly interacts with VDR (vitamin D receptor) via the VDR ligand-binding domain in a manner that does not require an LXXLL motif; DDX5 co-localizes with VDR in keratinocyte nuclei and acts as a co-activator for calcitriol-dependent transcription. This interaction parallels known DDX5 interactions with ERα and AR. Genome-wide protein-protein interaction screen using VDR as bait, domain analysis of VDR-DDX5 interaction, VDR helix 12 mutant analysis, co-localization in HaCaT cells, transcription reporter assays, shRNA knockdown Molecular and cellular endocrinology Medium 22476084
2018 DDX5 regulates microRNA biogenesis; LMTK3 binds via DDX5 to pri-miRNAs of miR-34a, miR-196-a2, and miR-182, sequestering them from processing. DDX5 is thus involved in the Microprocessor complex activity for miRNA processing. Co-immunoprecipitation of LMTK3-DDX5, pri-miRNA binding assay, miRNA expression profiling, functional proliferation/invasion assays Cancer letters Low 26739063
2013 DDX5 (p68) binds JEV core protein, NS3, and NS5 (MTase and RdRp domains) as shown by GST pulldown and Co-IP; DDX5 is recruited to the cytoplasm and co-localizes with viral proteins and RNA. DDX5 binds specifically to the JEV 3'UTR by RNA pulldown. Helicase activity is required for DDX5's pro-viral role; DDX5 knockdown reduces JEV replication but not virus assembly/release. GST pulldown, Co-immunoprecipitation, confocal co-localization, RNA pulldown, JEV-replicon system, siRNA knockdown with helicase mutants Antiviral research Medium 24035833
2024 MCM8 interacts with DDX5 and DHX9; loss of MCM8 reduces retention of DDX5 and DHX9 at R-loops, leading to R-loop accumulation and genome instability. MCM8 premature ovarian insufficiency-causative mutants with decreased DDX5 interaction display increased R-loop levels. Co-immunoprecipitation of MCM8-DDX5/DHX9, R-loop quantification in MCM8-deficient cells, MCM8 mutant interaction analysis, primordial germ cell proliferation assays in MCM8 KO mice The EMBO journal Medium 38858601
2018 DDX5 knockdown in basal breast cancer cells causes actin cytoskeleton reorganization via a DDX5→miR-182→actin cytoskeleton pathway; DDX5 regulates miR-182 (and miR-21) levels, and loss of miR-182 upregulates cofilin and profilin, key actin polymerization proteins. Treatment with miR-182 inhibitors phenocopies DDX5 knockdown. Quantitative proteomics, global miRNA profiling, DDX5 knockdown, miR-182 inhibitor treatment, actin cytoskeleton morphology analysis, PDCD4 (miR-21 target) upregulation assay Molecular & cellular proteomics Medium 22086602
2021 AURKA forms a transcriptional coactivator complex with DDX5 to induce transcription of lncRNA TMEM147-AS1 in epithelial ovarian cancer; this occurs via direct binding of AURKA to DDX5. The feedback loop AURKA/DDX5/TMEM147-AS1/let-7 maintains cisplatin resistance via lipophagy activation. Co-immunoprecipitation of AURKA-DDX5, transcription assays at TMEM147-AS1 promoter, functional cisplatin resistance assays, mathematical modeling Cancer letters Low 37217070
2018 DDX5 is O-GlcNAcylated by OGT; DDX5 directly interacts with OGT in SW480 cells, and O-GlcNAcylation promotes DDX5 protein stability. The OGT-DDX5 axis activates AKT/mTOR signaling to promote colorectal cancer progression. Co-immunoprecipitation of DDX5-OGT, O-GlcNAcylation assays, protein stability analysis (cycloheximide chase), AKT/mTOR pathway analysis, siRNA knockdown Journal of cellular and molecular medicine Low 30484950
2021 HSP90 interacts directly with DDX5 and inhibits DDX5 protein degradation through the AMPK/ULK1-regulated autophagy pathway; HSP90 inhibition reduces DDX5 levels and blocks HCC tumor growth. DDX5 accumulation activates β-catenin signaling. Molecular docking, co-immunoprecipitation of DDX5-HSP90, autophagy pathway analysis (AMPK/ULK1), HSP90 inhibitor experiments, xenograft tumor models Cancer biology & medicine Medium 33764710
2018 In oligodendrocytes, DDX5 localizes to heterogeneous cytoplasmic RNP complexes associated with Mbp mRNA in cell body and processes; DDX5 level inversely affects MBP protein level post-transcriptionally, and DDX5 knockdown increases MBP isoforms containing exon 2 (via alternative splicing regulation), indicating a dual role in translational repression and alternative splicing of Mbp. RNP complex immunoprecipitation with Mbp mRNA, DDX5 knockdown with MBP protein level measurement, alternative splicing analysis by RT-PCR, subcellular localization by immunofluorescence Journal of cell science Medium 29622601
2022 The m6A reader YTHDC1 interacts with DDX5 in rhabdomyosarcoma cells; DDX5 and YTHDC1 co-operatively promote production of a common subset of circRNAs by mediating back-splicing. YTHDC1/DDX5 depletion reduces RMS cell proliferation. Co-immunoprecipitation of YTHDC1-DDX5, circRNA sequencing, back-splicing assays, siRNA knockdown of YTHDC1/DDX5 with proliferation readout Nature communications Medium 37019933
2020 Genome-wide DRIP-seq mapping revealed that DDX5-, XRN2-, and PRMT5-deficient cells share many R-loop gain loci at transcription termination sites (consistent with coordinated RNA Pol II termination), but DDX5-depleted cells uniquely accumulate R-loops near transcription start sites, suggesting an independent role for DDX5 in transcription initiation. R-loop accumulation at certain loci in DDX5-deficient cells induces antisense intergenic transcription. DRIP-seq (genome-wide R-loop mapping) in DDX5-, XRN2-, and PRMT5-deficient cells, bioinformatic analysis of R-loop gain/loss loci Life science alliance Medium 32747416

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2018 The lncRNA NEAT1 activates Wnt/β-catenin signaling and promotes colorectal cancer progression via interacting with DDX5. Journal of hematology & oncology 325 30185232
2014 RNA helicases DDX5 and DDX17 dynamically orchestrate transcription, miRNA, and splicing programs in cell differentiation. Cell reports 168 24910439
2019 Arginine methylation of the DDX5 helicase RGG/RG motif by PRMT5 regulates resolution of RNA:DNA hybrids. The EMBO journal 151 31267554
2015 DDX5 and its associated lncRNA Rmrp modulate TH17 cell effector functions. Nature 138 26675721
2007 Redundant role of DEAD box proteins p68 (Ddx5) and p72/p82 (Ddx17) in ribosome biogenesis and cell proliferation. Nucleic acids research 131 17485482
2013 The DEAD box proteins DDX5 (p68) and DDX17 (p72): multi-tasking transcriptional regulators. Biochimica et biophysica acta 127 23523990
2019 DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia. Nature communications 110 31123254
2019 DDX5 helicase resolves G-quadruplex and is involved in MYC gene transcriptional activation. Proceedings of the National Academy of Sciences of the United States of America 106 31548374
2012 mrhl RNA, a long noncoding RNA, negatively regulates Wnt signaling through its protein partner Ddx5/p68 in mouse spermatogonial cells. Molecular and cellular biology 106 22665494
2012 DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells. Cancer discovery 106 22750847
2018 The DDX5/Dbp2 subfamily of DEAD-box RNA helicases. Wiley interdisciplinary reviews. RNA 96 30506978
2010 Multi-talented DEAD-box proteins and potential tumor promoters: p68 RNA helicase (DDX5) and its paralog, p72 RNA helicase (DDX17). American journal of translational research 95 20589163
2021 BRCA2 promotes DNA-RNA hybrid resolution by DDX5 helicase at DNA breaks to facilitate their repair‡. The EMBO journal 94 33634895
2008 The DEAD box RNA helicases p68 (Ddx5) and p72 (Ddx17): novel transcriptional co-regulators. Biochemical Society transactions 93 18631126
2015 DDX5 promotes proliferation and tumorigenesis of non-small-cell lung cancer cells by activating β-catenin signaling pathway. Cancer science 85 26212035
2019 LncRNA CCAT1 Promotes Prostate Cancer Cell Proliferation by Interacting with DDX5 and MIR-28-5P. Molecular cancer therapeutics 84 31387890
2018 Long non-coding RNA MIAT promotes gastric cancer growth and metastasis through regulation of miR-141/DDX5 pathway. Journal of experimental & clinical cancer research : CR 78 29540201
2018 Roles of DDX5 in the tumorigenesis, proliferation, differentiation, metastasis and pathway regulation of human malignancies. Biochimica et biophysica acta. Reviews on cancer 76 30419318
2022 IL-17D-induced inhibition of DDX5 expression in keratinocytes amplifies IL-36R-mediated skin inflammation. Nature immunology 73 36271146
2011 RNA helicase DDX5 regulates microRNA expression and contributes to cytoskeletal reorganization in basal breast cancer cells. Molecular & cellular proteomics : MCP 73 22086602
2020 LncRNA SLC26A4-AS1 suppresses the MRN complex-mediated DNA repair signaling and thyroid cancer metastasis by destabilizing DDX5. Oncogene 72 32939012
2020 DDX5 resolves R-loops at DNA double-strand breaks to promote DNA repair and avoid chromosomal deletions. NAR cancer 72 33015627
2008 p68 (Ddx5) interacts with Runx2 and regulates osteoblast differentiation. Journal of cellular biochemistry 66 17960593
2022 DDX5 and DDX17-multifaceted proteins in the regulation of tumorigenesis and tumor progression. Frontiers in oncology 64 35992805
2017 RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions. Cell stem cell 62 28111200
2022 Resolution of R-loops by topoisomerase III-β (TOP3B) in coordination with the DEAD-box helicase DDX5. Cell reports 61 35830799
2012 Dual role of the ddx5/ddx17 RNA helicases in the control of the pro-migratory NFAT5 transcription factor. Oncogene 61 22266867
2011 RNA helicase DDX5 is a p53-independent target of ARF that participates in ribosome biogenesis. Cancer research 61 21937682
2018 DDX5 RNA Helicases: Emerging Roles in Viral Infection. International journal of molecular sciences 59 29642538
2013 The DEAD-box RNA helicase DDX5 acts as a positive regulator of Japanese encephalitis virus replication by binding to viral 3' UTR. Antiviral research 59 24035833
2020 LINC01116 promotes tumor proliferation and neutrophil recruitment via DDX5-mediated regulation of IL-1β in glioma cell. Cell death & disease 58 32358484
2018 Coordinate regulation of alternative pre-mRNA splicing events by the human RNA chaperone proteins hnRNPA1 and DDX5. Genes & development 58 30042133
2008 P68 RNA helicase (DDX5) alters activity of cis- and trans-acting factors of the alternative splicing of H-Ras. PloS one 58 18698352
2020 Genome-wide R-loop analysis defines unique roles for DDX5, XRN2, and PRMT5 in DNA/RNA hybrid resolution. Life science alliance 57 32747416
2021 The RNA helicase DDX5 promotes viral infection via regulating N6-methyladenosine levels on the DHX58 and NFκB transcripts to dampen antiviral innate immunity. PLoS pathogens 56 33909701
2017 DDX5 promotes gastric cancer cell proliferation in vitro and in vivo through mTOR signaling pathway. Scientific reports 55 28216662
2018 The role of DEAD-box RNA helicase p68 (DDX5) in the development and treatment of breast cancer. Journal of cellular physiology 54 30417346
2021 LncRNA PRADX-mediated recruitment of PRC2/DDX5 complex suppresses UBXN1 expression and activates NF-κB activity, promoting tumorigenesis. Theranostics 53 33754075
2013 RNA helicase Ddx5 and the noncoding RNA SRA act as coactivators in the Notch signaling pathway. Biochimica et biophysica acta 52 23396200
2021 RNA helicase DDX5 enables STAT1 mRNA translation and interferon signalling in hepatitis B virus replicating hepatocytes. Gut 51 34021034
2018 Functions of DEAD box RNA helicases DDX5 and DDX17 in chromatin organization and transcriptional regulation. BMB reports 50 30293550
2013 DDX5 facilitates HIV-1 replication as a cellular co-factor of Rev. PloS one 49 23741449
2013 The Ddx5 and Ddx17 RNA helicases are cornerstones in the complex regulatory array of steroid hormone-signaling pathways. Nucleic acids research 48 24275493
2018 O-GlcNAcylation promotes colorectal cancer progression by regulating protein stability and potential catcinogenic function of DDX5. Journal of cellular and molecular medicine 45 30484950
2023 The m6A reader YTHDC1 and the RNA helicase DDX5 control the production of rhabdomyosarcoma-enriched circRNAs. Nature communications 44 37019933
2020 Restoration of RNA helicase DDX5 suppresses hepatitis B virus (HBV) biosynthesis and Wnt signaling in HBV-related hepatocellular carcinoma. Theranostics 44 33042264
2014 The expanding functions of cellular helicases: the tombusvirus RNA replication enhancer co-opts the plant eIF4AIII-like AtRH2 and the DDX5-like AtRH5 DEAD-box RNA helicases to promote viral asymmetric RNA replication. PLoS pathogens 43 24743583
2012 The DEAD-box RNA helicase DDX3 interacts with DDX5, co-localizes with it in the cytoplasm during the G2/M phase of the cycle, and affects its shuttling during mRNP export. Journal of cellular biochemistry 43 22034099
2013 p68/DdX5 supports β-catenin & RNAP II during androgen receptor mediated transcription in prostate cancer. PloS one 40 23349811
2024 DDX5 inhibits hyaline cartilage fibrosis and degradation in osteoarthritis via alternative splicing and G-quadruplex unwinding. Nature aging 39 38760576
2022 FL118, acting as a 'molecular glue degrader', binds to dephosphorylates and degrades the oncoprotein DDX5 (p68) to control c-Myc, survivin and mutant Kras against colorectal and pancreatic cancer with high efficacy. Clinical and translational medicine 39 35604033
2014 Arf tumor suppressor disrupts the oncogenic positive feedback loop including c-Myc and DDX5. Oncogene 36 24469041
2023 Role of the DEAD-box RNA helicase DDX5 (p68) in cancer DNA repair, immune suppression, cancer metabolic control, virus infection promotion, and human microbiome (microbiota) negative influence. Journal of experimental & clinical cancer research : CR 35 37596619
2018 miR-5590-3p inhibited tumor growth in gastric cancer by targeting DDX5/AKT/m-TOR pathway. Biochemical and biophysical research communications 34 30029874
2019 Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis. Artificial cells, nanomedicine, and biotechnology 33 31379208
2014 Acquired dependence of acute myeloid leukemia on the DEAD-box RNA helicase DDX5. Cell reports 33 24910429
2021 LncPSCA in the 8q24.3 risk locus drives gastric cancer through destabilizing DDX5. EMBO reports 31 34472665
2020 Long Noncoding RNA NHEG1 Drives β-Catenin Transactivation and Neuroblastoma Progression through Interacting with DDX5. Molecular therapy : the journal of the American Society of Gene Therapy 31 31982037
2013 The RNA helicase Ddx5/p68 binds to hUpf3 and enhances NMD of Ddx17/p72 and Smg5 mRNA. Nucleic acids research 31 23788676
2012 The RNA helicase DDX5/p68 is a key factor promoting c-fos expression at different levels from transcription to mRNA export. Nucleic acids research 31 23143267
2022 DDX5/METTL3-METTL14/YTHDF2 Axis Regulates Replication of Influenza A Virus. Microbiology spectrum 30 35583334
2019 Hepatoma-Derived Growth Factor and DDX5 Promote Carcinogenesis and Progression of Endometrial Cancer by Activating β-Catenin. Frontiers in oncology 30 31032220
2023 RNA helicase DDX5 modulates sorafenib sensitivity in hepatocellular carcinoma via the Wnt/β-catenin-ferroptosis axis. Cell death & disease 29 38036507
2021 Multiple functions of the DEAD-box RNA helicase, DDX5 (p68), make DDX5 a superior oncogenic biomarker and target for targeted cancer therapy. American journal of cancer research 29 34765320
2018 DDX5 promotes hepatocellular carcinoma tumorigenesis via Akt signaling pathway. Biochemical and biophysical research communications 29 30119889
2015 LMTK3 escapes tumour suppressor miRNAs via sequestration of DDX5. Cancer letters 29 26739063
2014 The RNA helicase/transcriptional co-regulator, p68 (DDX5), stimulates expression of oncogenic protein kinase, Polo-like kinase-1 (PLK1), and is associated with elevated PLK1 levels in human breast cancers. Cell cycle (Georgetown, Tex.) 29 24626184
2009 A DDX5 S480A polymorphism is associated with increased transcription of fibrogenic genes in hepatic stellate cells. The Journal of biological chemistry 29 20022962
2021 Thrap3 promotes R-loop resolution via interaction with methylated DDX5. Experimental & molecular medicine 28 34697388
2016 Knockdown of DDX5 Inhibits the Proliferation and Tumorigenesis in Esophageal Cancer. Oncology research 27 28244855
2021 PAK5 promotes RNA helicase DDX5 sumoylation and miRNA-10b processing in a kinase-dependent manner in breast cancer. Cell reports 25 34936874
2018 RNA helicase DDX5 participates in oxLDL-induced macrophage scavenger receptor 1 expression by suppressing mRNA degradation. Experimental cell research 25 29522752
2020 DDX5 promotes oncogene C3 and FABP1 expressions and drives intestinal inflammation and tumorigenesis. Life science alliance 24 32817263
2020 Circ-XPR1 promotes osteosarcoma proliferation through regulating the miR-214-5p/DDX5 axis. Human cell 24 32920730
2023 AURKAIP1 actuates tumor progression through stabilizing DDX5 in triple negative breast cancer. Cell death & disease 22 38040691
2022 DEAD-Box RNA Helicases DDX3X and DDX5 as Oncogenes or Oncosuppressors: A Network Perspective. Cancers 22 35954483
2022 DDX5 mRNA-targeting antisense oligonucleotide as a new promising therapeutic in combating castration-resistant prostate cancer. Molecular therapy : the journal of the American Society of Gene Therapy 22 35965411
2021 LncRNA SNHG14 promotes cell proliferation and invasion in colorectal cancer through modulating miR-519b-3p/DDX5 axis. Journal of Cancer 22 34234865
2020 RNA helicase DDX5 suppresses IFN-I antiviral innate immune response by interacting with PP2A-Cβ to deactivate IRF3. Experimental cell research 22 33065113
2023 The feedback loop of AURKA/DDX5/TMEM147-AS1/let-7 drives lipophagy to induce cisplatin resistance in epithelial ovarian cancer. Cancer letters 21 37217070
2020 DDX5 potentiates HIV-1 transcription as a co-factor of Tat. Retrovirology 21 32228614
2024 MCM8 interacts with DDX5 to promote R-loop resolution. The EMBO journal 20 38858601
2019 Endogenous interaction profiling identifies DDX5 as an oncogenic coactivator of transcription factor Fra-1. Oncogene 20 31015574
2012 DDX5 is a multifunctional co-activator of steroid hormone receptors. Molecular and cellular endocrinology 20 22476084
2010 Role of multiple HLR1 sequences in the regulation of the dual promoters of the psaAB genes in Synechocystis sp. PCC 6803. Journal of bacteriology 20 20511509
2023 RNA binding protein DDX5 restricts RORγt+ Treg suppressor function to promote intestine inflammation. Science advances 19 36724232
2021 RNA binding protein DDX5 directs tuft cell specification and function to regulate microbial repertoire and disease susceptibility in the intestine. Gut 19 34853057
2025 Acetyltransferase NAT10 inhibits T-cell immunity and promotes nasopharyngeal carcinoma progression through DDX5/HMGB1 axis. Journal for immunotherapy of cancer 18 39939141
2022 seRNA PAM controls skeletal muscle satellite cell proliferation and aging through trans regulation of Timp2 expression synergistically with Ddx5. Aging cell 18 35851988
2021 RNA helicase DDX5 acts as a critical regulator for survival of neonatal mouse gonocytes. Cell proliferation 17 33666296
2021 Heat shock protein 90 promotes RNA helicase DDX5 accumulation and exacerbates hepatocellular carcinoma by inhibiting autophagy. Cancer biology & medicine 17 33764710
2018 Dual role of the RNA helicase DDX5 in post-transcriptional regulation of myelin basic protein in oligodendrocytes. Journal of cell science 17 29622601
2022 DDX5: an expectable treater for viral infection- a literature review. Annals of translational medicine 16 35845539
2021 Silencing of Long Non-Coding RNA FGD5-AS1 Inhibits the Progression of Non-Small Cell Lung Cancer by Regulating the miR-493-5p/DDX5 Axis. Technology in cancer research & treatment 16 33550957
2021 MSC-AS1 induced cell growth and inflammatory mediators secretion through sponging miR-142-5p/DDX5 in gastric carcinoma. Aging 16 33819916
2020 The RNA helicase DDX5 supports mitochondrial function in small cell lung cancer. The Journal of biological chemistry 16 32376686
2018 DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV. Journal of molecular biology 16 30131116
2024 DDX5 inhibits inflammation by modulating m6A levels of TLR2/4 transcripts during bacterial infection. EMBO reports 15 38182816
2024 RNA Helicase DDX5 Maintains Cardiac Function by Regulating CamkIIδ Alternative Splicing. Circulation 15 39056171
2024 Circular RNA IGF1R Promotes Cardiac Repair via Activating β-Catenin Signaling by Interacting with DDX5 in Mice after Ischemic Insults. Research (Washington, D.C.) 15 39193132

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